• iFluor™ superior fluorescent labelling dyes, optimised for labelling proteins and nucleic acids. (stratech.co.uk)
  • Electrokinetic techniques are contact-free methods currently used in many applications, where precise handling of biological entities, such as cells, bacteria or nucleic acids, is needed. (ibecbarcelona.eu)
  • At the same time, because it does not precipitate with metals,and with little interference with biochemical processes,tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many other important uses. (vacutaineradditives.com)
  • The Hammond lab has a dual focus on engineering nucleic acids as programmable tools for molecular imaging and gene control, and on understanding the chemistry and biology of cyclic dinucleotides as signaling molecules in bacteria and mammalian cells. (utah.edu)
  • Some of them interact and form coordination complexes with nucleic acids, forming coordination complexes with nucleic acids, causing various DNA lesions (Theophanides and Anastassopoulou 2017 ). (springeropen.com)
  • Here we introduce a platform for the detection of nucleic acids based on a magnetic barcoding strategy. (cdc.gov)
  • Hubei Xindesheng Material Co.,ltd is a high-tech company specializing in the research and development, production and sales of biological buffers, blood collection tube additives, chemiluminescence reagents and luminescent substrates. (vacutaineradditives.com)
  • The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. (patsnap.com)
  • Since the introduction of the first bioluminescent dual-luciferase assay in 1995, this approach has been used in countless studies to advance our scientific understanding of cellular gene regulation. (promegaconnections.com)
  • Particular importance here is the biological applications of Pt NCs such as the bioimaging of various cells as a preferred fluorophore in contrast to traditional fluorescent markers (e.g., organic dye, semiconductor quantum dots, and fluorescent proteins), the usage of Pt NCs-based antitumour drugs as a new class chemotherapeutics for malignant tumour therapy, and the utilization of antibacteria as an alternative of Ag-based antibacterial agent. (hindawi.com)
  • mPEG-SCM (Amine PEGylation of Proteins) - PEGylation of lysine residues improves the proteolytic stability of fibronectin while retaining biological activity, Biotechnol. (creativepegworks.com)
  • AAT Bioquest are well known for their photometric detection fluorescence and luminescent technologies. (stratech.co.uk)
  • FP Fluor™ superior fluorescent labelling dyes, optimised for developing fluorescence polarisation-based assays. (stratech.co.uk)
  • By screening for enzyme activity using an in vivo biosensor assay, we subsequently discovered a G. sulfurreducens GGDEF enzyme that makes cGAMP, which is the founding member of Hypr GGDEF enzymes. (utah.edu)
  • We have analyzed the activity of cyclic di-GMP and cyclic di-AMP signaling enzymes using in vivo biosensor assays. (utah.edu)
  • Effects of circPAPD4 overexpression on tumor progression were investigated using in vivo assays. (biomedcentral.com)
  • A membrane receptor reagent and assay is disclosed in which liposomes are bound to an evanescent wave emitting surface. (google.com)
  • They can be performed easily by adding a reagent that provides components necessary to generate a luminescent signal directly to cells or a cell lysate. (promegaconnections.com)
  • The ROS-ID ® ROS/RNS detection kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. (enzolifesciences.com)
  • however, the study of ultrafine Pt NCs is at the beginning stage and there are still plenty of challenges like synthesis of near-infrared (NIR) fluorescent Pt NCs, the explicit signal pathway of cell apoptosis, and attempt in diverse biological applications that need to be urgently tackled in future. (hindawi.com)
  • Cell proliferation and apoptosis were measured using CCK8, EdU staining, flow cytometry, and TUNEL assays. (biomedcentral.com)
  • COLD SPRING HARBOR, NEW YORK -- In an important, comprehensive, and timely review, an expert team from the University of California Berkeley details the methodologies used in nucleic acid-based tests for detecting the presence of SARS-CoV-2, the virus that causes COVID-19. (cshlpress.com)
  • Most recently, GeneXpert MTB/RIF (Cepheid) advanced nucleic acid-based detection by integrating PCR and a highly sensitive molecular-beacon assay into a single, automated system. (cdc.gov)
  • Carbon Dots (CDs) are luminescent quasi-spherical nanoparticles, possessing water solubility, high biocompatibility, and tunable chemical and physical properties for a wide range of applications, including nanomedicine and theranostics. (researchgate.net)
  • Y. Nakajima and Y. Ohmiya, Bioluminescence assays: multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. (atto.co.jp)
  • M. Yasunaga, Y. Nakajima and Y. Ohmiya, Dual-color bioluminescence imaging assay using green- and red-emitting beetle luciferases at subcellular resolution. (atto.co.jp)
  • bioluminescence is a biological process only found in certain species of organisms. (thenoveldifference.com)
  • Bioluminescence is a biological phenomenon where living organisms generate visible light by an enzymatic reaction inside their bodies. (thenoveldifference.com)
  • Bioluminescence is a biological process. (thenoveldifference.com)
  • Luciferase assays are useful tools for studying a wide range of biological questions. (promegaconnections.com)
  • In the past decades, gold and silver nanoclusters (Au NCs and Ag NCs) have been massively explored and utilized in the field of industrial catalysis, optoelectronic devices, biological imaging, environmental detection, clinical diagnoses, and treatment. (hindawi.com)
  • Limit of detection was 0.009 U/L and limit of quantitation was 0.027 U/L for the combined FAP-PREP assay. (inra.fr)
  • Nucleic acid amplification tests, in particular, have emerged as a preferred approach for TB detection. (cdc.gov)
  • We herein report on the development of a nucleic acid platform designed to provide fast and portable detection, and demonstrated the utility by detecting MTB DNA from mechanically processed TB patient sputum samples. (cdc.gov)
  • Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)
  • A microfluidic system for performing chemical reactions or biochemical, biological, or chemical assays utilizing a microfabricated device or "chip. (patsnap.com)
  • Functionalities can be added to nanomaterials by interfacing them with biological molecules or structures. (wikipedia.org)
  • This class of small molecules, referred to as pyridopyrimidinones (PDPMs), showed a wide range of biological activities. (cdc.gov)
  • The presence of pollutants in soil and water has given rise to diverse analytical and biological approaches to detect and measure contaminants in the environment. (springeropen.com)
  • The specificity of the assay is confirmed by a panel of clinically relevant non-MTB bacteria, and the clinical utility is demonstrated by the measurements in MTB-positive patient specimens. (cdc.gov)
  • Nanomedicine ranges from the medical applications of nanomaterials and biological devices, to nanoelectronic biosensors, and even possible future applications of molecular nanotechnology such as biological machines. (wikipedia.org)
  • This plasmid can be extracted and bound specifically to a molecular target (i.e. protein, polysaccharide, lipid, or nucleic acid) immobilized covalently or non-covalently on a paramagnetic metallic nanoparticle. (conceptualmindworks.com)
  • The breast cancer MDA-MB-231 and ZR-75-1 cell lines were analyzed using MTT assay, flow cytometry, and spectrophotometric methods. (mdpi.com)
  • The continuous flow of probes and shaping liquid on these selected cells resulted in a 120-fold reduction of the hybridization time compared with the standard protocol (3 min vs. 6 h) and efficient rinsing, thereby shortening the total FISH assay time for centromeric probes. (springer.com)
  • Cell Meter™ cellular functional assay kits, a complete set of tools for functional analysis of cellular events. (stratech.co.uk)
  • Orangu ™ is a non-cytotoxic, sensitive, colorimetric assay for the determination of viable cell numbers in cell proliferation and cytotoxicity assays. (biolynx.ca)
  • Treatment of MX-1 and MCF7 cells (Wip1amplified, p53 wild type) with GSK 2830371 (0.001, 0.01, 0.1, 1, and 10 μM) causes concentration-dependent effects in cell growth assays [1] . (medchemexpress.com)
  • Advanced environmental controls, including CO 2 /O 2 control, incubation to 70 °C, and variable shaking support live cell assays. (agilent.com)
  • We recently developed a biosensor-based platereader assay to quantitate cGAMP levels in DNA-stimulated mammalian cell lysates. (utah.edu)
  • Tris is also an important raw material for the synthesis of another biological buffer, TAPS.The advantages of Tris are very obvious. (vacutaineradditives.com)
  • The present invention provides an analytical test device for conducting assays of biological fluids. (patsnap.com)
  • Methods for carrying out the assays with the disclosed analytical test device are also provided. (patsnap.com)
  • Transient transfection is often used to perform reporter assays. (promegaconnections.com)
  • Here are seven ways in which dual-reporter assays help you avoid misinterpreting results. (promegaconnections.com)
  • The variations in transfection efficiency will equally affect both the experimental and control reporters so the ratio of activity in dual-reporter assays will normalize the data. (promegaconnections.com)
  • Often, reporter assays look at the dose response curve of a particular compound with regard to gene expression. (promegaconnections.com)
  • Ideally, a plate should be equilibrated to ambient room temperature before proceeding to the reporter assay. (promegaconnections.com)
  • Promega dual-reporter assays are designed for same-well multiplexing so there is no chance of variations creeping into your data due to unequal splitting of the cellular lysate to measure two separate reporter activities. (promegaconnections.com)
  • i) Cyclic AMP-GMP (Bacterial cGAMP) signaling: Using an in vitro biosensor assay, we discovered a cyclic AMP-GMP (cGAMP)-sensing riboswitch class, which was highlighted by Science Signaling as a Signaling Breakthrough of the Year . (utah.edu)
  • It is often employed in glowing sticks, chemiluminescent tests in research (such as the enzyme-linked immunosorbent assays (ELISAs)), and in certain kinds that use artificial light. (thenoveldifference.com)
  • It is an isotonic buffered saline solution commonly used in biology, such as experiments in immunohistochemistry, situ hybridization, enzyme-linked immunosorbent assay. (vacutaineradditives.com)
  • Its pathophysiological mechanisms involve various biological events such as neuronal apoptosis, oxidative stress, endoplasmic reticulum stress and inflammatory cascade[ 2 - 5 ]. (ijpsonline.com)
  • Current assays inadequately discriminate between FAP and PREP and there is need for an assay that reliably quantitates the FAP/PREP activity ratio in plasma. (inra.fr)
  • Halliwell JA, Gravells P & Bryant HE (2020) DNA fiber assay for the analysis of DNA replication progression in human pluripotent stem cells . (sheffield.ac.uk)
  • It represents the culmination of basic and applied research to solve the problem of generating a biological system that can detect, identify and neutralize target biological agents in a seamless manner. (conceptualmindworks.com)
  • However, despite growing biological and clinical significance, little is known about the structural arrangement of this receptor or any of its family members. (uci.edu)
  • RESULTS: A combined kinetic assay was developed in conditions to optimally measure FAP as well as PREP activity with Z-Gly-Pro-AMC as substrate. (inra.fr)
  • CONCLUSION: With this new assay, on average 67% of the Z-Gly-Pro-AMC converting activity in plasma can be attributed to FAP. (inra.fr)
  • More than 95% of this activity is inhibited in assays where substrate has been added to a preincubated mixture of tissue with the non-neuropathic organophosphorus compound (OP) paraoxon (O,O'-diethyl p-nitrophenyl phosphate): residual activity includes soluble neuropathy target esterase (S-NTE) which, by definition, is considered resistant to long-term progressive (covalent) inhibition by paraoxon. (inrae.fr)
  • Based on the above excellent results, GSH-related biological assays have been further established by taking ATP as a model. (bvsalud.org)
  • μFISH enables a rapid nucleic acid analysis, and we demonstrate its usefulness for spatially multiplexed FISH, Fig. 1 . (springer.com)
  • Additionally, this study will help define the core symptoms of PASC to assist researchers in identifying the biological causes of the phenomenon. (asm.org)
  • Tris, also known as Tris (Hydroxymethyl) Aminomethane, Tris buffer is widely used, especially in nucleic acid and research experiments related to DNA, it has much more advantages than PBS phosphate buffer. (vacutaineradditives.com)
  • The analogue of Au and Ag NCs and platinum nanoclusters (Pt NCs), especially their biological applications, is relatively and rarely discussed. (hindawi.com)
  • Combined with the high catalytic oxidation ability of DNAzyme, this ECL sensor can realize ATP assay as low as 1.4 fmol without other complicated exonuclease amplification strategies. (bvsalud.org)
  • Combined with portable systems, the magnetic barcode assay holds promise to become a sensitive, high-throughput, and low-cost platform for point-of-care diagnostics. (cdc.gov)
  • and freezing the said biological material using any protocol applicable thereto. (patsnap.com)
  • Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence. (patsnap.com)
  • Gen-Probe RNA test, interferon-gamma assay, urine-based antigen test). (cdc.gov)
  • This work outlines a femtomolar-level and highly selective glutathione (GSH) and adenosine triphosphate (ATP) ECL assay strategy using a facile split-type gold nanocluster (AuNC) probe-based ECL platform. (bvsalud.org)
  • AAT strive to innovate and push the boundaries of assay technology. (stratech.co.uk)
  • With a patented combination of monochromator and filter optics, Synergy Neo2 an advanced plate reader delivering flexibility and performance for any microplate assay. (agilent.com)