FluorescenceAcidsPeptide nuclFluorescentProbesDetectionProbeAssaySequencesMolecularFISHMiRNAChromosomeSensitivityDiagnosisChain reactionApproachesGENE EXPRESSIONConcentrationTissue sectionsPrecipitateLaboratoriesAnalysisStainDiagnosticTechniquesStructuresIsolationAcademicSpeciesPreparationDiseaseTranscriptionFactors
Fluorescence15
- Data acquisition in in situ hybridization experiments is performed using a fluorescence microscope or with a cell imaging system, such as INCELL Analyzer. (qima-lifesciences.com)
- Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. (wikipedia.org)
- Visualization of the mycelia of wood-rotting fungi by fluorescence in situ hybridization using a peptide nucleic acid probe. (bvsalud.org)
- White rot fungus , Phanerochaete chrysosporium , and brown rot fungus , Postia placenta , grown on agar plates, were visualized by fluorescence in situ hybridization (FISH) using a peptide nucleic acid (PNA) probe. (bvsalud.org)
- Therefore, we analyzed the potential of fluorescence in situ hybridization (FISH) for diagnosing WD, using a Tropheryma (T.) whipplei -specific probe. (frontiersin.org)
- We here present a micrometer-scale implementation of fluorescence in situ hybridization that we term μFISH. (springer.com)
- A key milestone in ISH-based techniques was fluorescence in situ hybridization (FISH) (Bauman et al. (springer.com)
- A short introduction how cytogenetics and molecular cytogenetics were established is followed by technical aspects of fluorescence in situ hybridization (FISH). (frontiersin.org)
- This review is about "molecular cytogenetics" including 1) the historical perspective of its development from cytogenetics, 2) technical aspects, 3) available probe sets, and 4) variants and applications of the basic fluorescence in situ hybridization (FISH) approach. (frontiersin.org)
- 2016). Application of locked nucleic acid-based probes in fluorescence in situ hybridization . (up.pt)
- 2015). Towards fluorescence in vivo hybridization (FIVH) detection of H. pylori in gastric mucosa using advanced LNA probes . (up.pt)
- 2017). Detection and discrimination of biofilm populations using locked nucleic acid/2'-O-methyl-RNA fluorescence in situ hybridization (LNA/2'OMe-FISH) . (up.pt)
- Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of mycobacterium immunogenum and DNA-FISH assay for analysis of pseudomonads in metalworking fluid s and sputum. (cdc.gov)
- Telomere length was determined by fluorescence in situ hybridisation in circulating lymphocytes harvested from 26 never-smokers, 24 smokers with normal lung function and 26 smokers with moderate-to-severe airflow obstruction (forced expiratory flow in one second 48±4% predicted). (ersjournals.com)
- Fluorescence in situ hybridization (FISH) is also an acceptable method, with ROS1positivity defined as the detection of at least 15% of neoplastic nuclei with ROS1 gene rearrangements among a minimum of 50 total neoplastic nuclei. (who.int)
Acids8
- Nucleic Acids Res. (harvard.edu)
- 2015). Erratum to: Mismatch discrimination in fluorescent in situ hybridization using different types of nucleic acids (Applied Microbiology and Biotechnology, 10.1007/s00253-015-6389-4) . (up.pt)
- Because this reagent binds to nucleic acids, it should be treated as a potential mutagen and handled with appropriate care. (aidstar-one.com)
- Flow cytometry in combination with fluorescent in situ hybridization (flow-FISH) is a powerful technique that can be utilized to rapidly detect nucleic acids at single-cell resolution without the need for homogenization or nucleic acid extraction. (nih.gov)
- These enzymes are present in many biological samples and can interfere with the isolation or analysis of nucleic acids. (pharma-industry-review.com)
- It digests protective proteins like histones or ribonucleoproteins and inactivates any remaining DNases or RNases that could degrade nucleic acids during extraction or purification. (pharma-industry-review.com)
- These features of nucleic acids enable us to design novel interaction pathways and functions by providing well-behaved molecular mechanisms. (caltech.edu)
- This thesis will first demonstrate the use of these nucleic acid molecular mechanisms in closed-loop genetic circuits and infectious disease testing using cgRNAs and HCR, respectively, then provide updated tools for the nucleic acid design community to exploit the programmable nature of nucleic acids. (caltech.edu)
Peptide nucl1
- A rapid protocol for the direct identification of Enterococcus faecalis and other Enterococcus species from a positive blood culture using a Peptide Nucleic Acid fluorescent in situ hybridization assay (PNA FISH). (jove.com)
Fluorescent1
- Its principle is based on the use of a fluorescent probe which represents a nucleic acid sequence specific to a target-gene. (qima-lifesciences.com)
Probes10
- The continuous flow of probes and shaping liquid on these selected cells resulted in a 120-fold reduction of the hybridization time compared with the standard protocol (3 min vs. 6 h) and efficient rinsing, thereby shortening the total FISH assay time for centromeric probes. (springer.com)
- It relies on the sequence-specific hybridization of probes to their complementary targets in individual cells, followed by direct or indirect detection of the labelled probe. (springer.com)
- DIG is used to label nucleic acid probes for applications such as in situ hybridization. (vectorlabs.com)
- Here, we describe a microfluidic-based method which enables the detection of microRNAs or miRNAs in single intact cells by flow-FISH using locked nucleic acid (LNA)-containing probes. (nih.gov)
- AMPIVIEW™ EGFR (AS) Dig RNA Probes are digoxigenin-labeled RNA probes targeting EGFR nucleic acid (DNA/RNA or RNA) for in situ hybridization in tissues and cells. (enzolifesciences.com)
- The probes are formulated in a buffered formamide solution with hybridization enhancers. (enzolifesciences.com)
- Detecting gamma-irradiated SARS-CoV-2 virions in an extraction buffer, the current amplified HCR lateral flow assay achieves a limit of detection of 200 copies/µL using nucleic acid probes to target the SARS-CoV-2 RNA genome. (caltech.edu)
- By swapping out nucleic acid probes to target different pathogens, amplified HCR lateral flow assays offer a platform for adaptable and sensitive at-home testing for emergent diseases. (caltech.edu)
- BioGenex has developed proprietary Super Sensitive Nucleic Acid (SSNA) miRNA probes that enhance signals from intrinsically low populated miRNAs. (biogenex.com)
- BioGenex is pleased to launch two Super Sensitive TM Nucleic Acid miRNA in situ hybridization (SSNA miRNA ISH) probes and automated systems for differentiation of wild type and BRCA mutated breast cancer. (biogenex.com)
Detection4
- Nucleic acid labeling is widely used by end-users for the detection of molecular infectious diseases. (technavio.com)
- The advent of the nucleic acid labeling technique, coupled with the development of genomics, has significantly increased the use of molecular biology enzymes, kits, and reagents for the detection of pathogenic events at the genomic level. (technavio.com)
- The lack of adequate training and courses on nucleic acid labeling tests available for the detection and staging of a disease is one of the prime factors limiting the number of students graduating to become laboratory technicians. (technavio.com)
- We then used the hybridization chain reaction mechanism to develop an adaptable and sensitive test for the detection of SARS-CoV-2. (caltech.edu)
Probe5
- These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane. (aidstar-one.com)
- In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. (aidstar-one.com)
- The initial and important step of FISH, CISH and ISH is to prepare your tissue for hybridization, before adding probe. (ahdiagnostics.dk)
- Briefly, a suspension containing a mixture of 2×10 6 lymphocytes and 2×10 6 of 1301 cells was denatured at 82°C for 10 min, with and without the fluorescein-conjugated PNA telomere probe in the hybridisation solution. (ersjournals.com)
- In situ hybridization with a blotin-labeled DNA probe was used to detect wheatrye translocations. (illinois.edu)
Assay3
- By using a novel RT in situ PCR assay, viral nucleic acid was localized to the cytoplasm of large numbers of tissue leukocytes and vascular endothelium in tissues with hemorrhage and to vessels, demonstrating acute intimal and medial necrosis. (usda.gov)
- Here, we enhance sensitivity by developing an amplified lateral flow assay that incorporates isothermal, enzyme-free signal amplification based on the mechanism of hybridization chain reaction (HCR). (caltech.edu)
- Although numerous methods are available for gaining information about miRNA signatures, in situ hybridization (ISH) assay is a powerful tool that allows direct assessment of malignant cells. (biogenex.com)
Sequences4
- A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. (nih.gov)
- Hybridization is a basic property of nucleotide sequences and is taken advantage of in numerous molecular biology techniques. (wikipedia.org)
- In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome). (wikipedia.org)
- Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. (bvsalud.org)
Molecular6
- In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. (wikipedia.org)
- DNA replication and transcription of DNA into RNA both rely upon nucleotide hybridization, as do molecular biology techniques including Southern blots and Northern blots, the polymerase chain reaction (PCR), and most approaches to DNA sequencing. (wikipedia.org)
- While older, PCR continues to be innovated and remains a central tool in molecular diagnostics laboratories of all kinds, and in situ hybridization. (kaloramainformation.com)
- Standard chromosome analysis by G-banding has a limited resolution, but molecular cytogenetic techniques, such as multi-subtelomeric FISH, microdeletion FISH, multicolour FISH and comparative genomic hybridisation (CGH), have played an important role for the diagnosis of MR during the past decade. (bmj.com)
- HCR is a molecular mechanism for in vitro and in situ amplification of signals to spatially identify proteins, RNA, or DNA in a sample. (caltech.edu)
- NUPACK is a growing software suite for the analysis and design of nucleic acid structures, devices, and systems serving the needs of researchers in the fields of nucleic acid nanotechnology, molecular programming, synthetic biology, and across the life sciences. (caltech.edu)
FISH1
- μFISH enables a rapid nucleic acid analysis, and we demonstrate its usefulness for spatially multiplexed FISH, Fig. 1 . (springer.com)
MiRNA1
- Paraffin-embedded glioma specimens were used for MiRNA locked nucleic acid (LNA) in situ hybridization and immunohistochemistry, and frozen glioma specimen were used for Real-time PCR. (biomedcentral.com)
Chromosome1
- Three-color in situ hybridization was applied to the study of numerical chromosome abnormalities as occur in human solid tumors. (nih.gov)
Sensitivity1
- Since those original observations, many refinements have increased the versatility and sensitivity of the procedure to the extent that in situ hybridization is now considered an essential tool in cytogenetics. (wikipedia.org)
Diagnosis1
- In recent years, end-users such as hospitals, diagnostic centers, academic institutes, and research organizations have been increasingly opting for nucleic acid labeling techniques for disease diagnosis. (technavio.com)
Chain reaction2
- A variety of different methods use hybridization to pinpoint the origin of a DNA sample, including the polymerase chain reaction (PCR). (wikipedia.org)
- Two examples of these mechanisms are the conditional guide RNA (cgRNA) and the hybridization chain reaction (HCR). (caltech.edu)
Approaches1
- This nucleic acid labeling market research report provides valuable insights on the post COVID-19 impact on the market, which will help companies evaluate their business approaches. (technavio.com)
GENE EXPRESSION1
- in situ hybridization can also be of interest for the analysis of specific gene expression profiles in sensitive samples, where total RNA extraction by RT-qPCR may not provide satisfactory results. (qima-lifesciences.com)
Concentration1
- Dilutions and concentration optimization can be done with AMPIVIEW™ Hybridization Buffer (1X), included in this set. (enzolifesciences.com)
Tissue sections2
- This facilitates the optimal preparation of tissue sections for in situ hybridization. (pharma-industry-review.com)
- In Situ Hybridization (ISH) is a powerful technique that allows you to identify and localize nucleic acid targets in formalin-fixed tissue sections and cells. (ahdiagnostics.dk)
Precipitate1
- As a result of the formation of a brown precipitate over sites of hybridization in this technique, the rye chromosomes were entirely brown in color, whereas the wheat chromosomes appeared blue. (illinois.edu)
Laboratories1
- Such research studies on genome sequencing and genetics, in turn, increase the adoption of nucleic acid labeling by research laboratories. (technavio.com)
Analysis2
- Bioalternatives can assist you in the implementation and in the analysis of your in situ hybridization experiments. (qima-lifesciences.com)
- Multiplexed analysis of fixed tissue RNA using Ligation in situ Hybridization. (harvard.edu)
Stain1
- Blue stain shows EEEV nucleic acid in the perikaryon and dendrites. (cdc.gov)
Diagnostic1
- Nucleic acid labeling needs skilled professionals such as pathologists and clinicians who can perform nucleic acid labeling tests, genotyping tests, and predisposition diagnostic tests. (technavio.com)
Techniques3
- These institutes make use of nucleic acid labeling techniques to conduct their R&D activities. (technavio.com)
- The increasing investment in the development of new biotechnological techniques is a nucleic acid labeling market trend that is expected to have a positive impact in the coming years. (technavio.com)
- Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques. (aidstar-one.com)
Structures1
- Guanine (G)-quadruplexes are highly stable nucleic acid secondary structures formed from short tracts of G-rich sequence associating together. (nature.com)
Isolation1
- ISH is performed without isolation of the targets from their source, i.e., in situ , and is widely used in research and diagnostics. (springer.com)
Academic2
- The increasing research and development (R&D) activities at academic institutes and medical organizations is notably driving the nucleic acid labeling market growth, although factors such as a lack of skilled workforce may impede the market growth. (technavio.com)
- One of the key factors driving growth in the nucleic acid labeling market is the increasing research and development (R&D) activities at academic institutes and medical organizations. (technavio.com)
Species1
- Overall, genetic relatedness of two species can be determined by hybridizing segments of their DNA (DNA-DNA hybridization). (wikipedia.org)
Preparation1
- The benefits of Proteinase K extend beyond nucleic acid preparation. (pharma-industry-review.com)
Disease1
- Such increasing use of nucleic acid labeling for exploring disease-related genes and management of infectious diseases is expected to positively impact the growth of the global nucleic acid labeling market. (technavio.com)
Transcription1
- We identified EEEV by reverse transcription real-time PCR and in situ hybridization. (cdc.gov)
Factors2
- Such factors are thus expected to propel the growth of the global nucleic acid labeling market. (technavio.com)
- Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. (aidstar-one.com)