• proteins
  • Using multiparameter flow cytometry we have measured the nuclear DNA content of exponentially growing HL-60 cells in conjunction with protein content, nuclear forward light scatter, DNA in situ sensitivity to denaturation, DNA accessibility to 7-aminoactinomycin D (7-AMD), and content of the proliferation-associated proteins: cyclin (PCNA), p105, p34, and Ki-67. (docphin.com)
  • These changes indicate that during S phase chromatin progressively condenses and suggest that the condensation is associated with the efflux of nonhistone proteins from the nucleus. (docphin.com)
  • Activation of these proteins leads to invasion of the basement membrane and cell proliferation associated with further angiogenesis. (wikipedia.org)
  • membrane
  • It shows an unusual subcellular localization pattern, being found at both the plasma membrane and in the nucleus. (wikipedia.org)
  • Because the presence of PTPkappa at the cell membrane was shown to sequester β-catenin to the plasma membrane, these data suggest that one mechanism whereby PTPkappa functions as a tumor suppressor is by regulating the intracellular localization of free-β-catenin. (wikipedia.org)
  • The authors suggest that the presence of PTPkappa at the plasma membrane in association with the cadherin/catenin complex is important for the maintenance of adherens junction in pancreatic acinar cells, much as it was suggested above in melanoma cells. (wikipedia.org)
  • TNFR1 is expressed in most tissues, and can be fully activated by both the membrane-bound and soluble trimeric forms of TNF, whereas TNFR2 is found typically in cells of the immune system, and respond to the membrane-bound form of the TNF homotrimer. (wikipedia.org)
  • This pathway begins at the cell membrane surface, where a ligand binds to its specific receptor. (wikipedia.org)
  • Ang binds to actin of both smooth muscle and endothelial cells to form complexes that activate proteolytic cascades which upregulate the production of proteases and plasmin that degrade the laminin and fibronectin layers of the basement membrane. (wikipedia.org)
  • Degradation of the basement membrane and extracellular matrix allows the endothelial cells to penetrate and migrate into the perivascular tissue. (wikipedia.org)
  • protein
  • In the present study, we aimed to investigate the role of syndecan-1 in tumor cell adhesion and migration, with special focus on the importance of its distinct protein domains, to better understand the structure-function relationship of syndecan-1 in tumor progression. (jove.com)
  • A decrease of the protein/DNA ratio, lowered DNA accessibility to 7-AMD, increased sensitivity of DNA to denaturation, and increased ability of isolated nuclei to scatter light all paralleled cell progression through S phase. (docphin.com)
  • Use of short interfering RNA (siRNA) of PTPkappa to reduce PTPkappa protein expression in the mammary epithelial cell line, MCF10A, resulted in increased cell proliferation. (wikipedia.org)
  • During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. (wikipedia.org)
  • Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). (wikipedia.org)
  • The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. (wikipedia.org)
  • Protein Ki-67 in human MCF-7 cells. (wikipedia.org)
  • Ki-67 protein (red), tubulin (green) and DNA (blue) in HeLa cells. (wikipedia.org)
  • Gene knockdown is achieved by preventing cells from making a targeted protein. (wikipedia.org)
  • inhibits
  • Belimumab (trade name Benlysta, previously known as LymphoStat-B) is a human monoclonal antibody that inhibits B-cell activating factor (BAFF), also known as B-lymphocyte stimulator (BLyS). (wikipedia.org)
  • bone marrow-derived
  • By expressing a dominant negative form of PTPkappa or by using short-hairpin RNA for PTPkappa in bone-marrow derived stem cells, Erdenbayer and colleagues demonstrated that CD4(+) T cells development was inhibited. (wikipedia.org)
  • vascular smooth muscl
  • The vasculature is predominantly comprised of vascular smooth muscle cells (VSMCs), and these cells are involved in the maintenance of vessel tone and blood pressure ( 1 , 2 ). (spandidos-publications.com)
  • In addition, reactive oxygen species (ROS)-dependent STAT3 activation has been established to be a key downstream mediator of PDGFR signalling in vascular smooth muscle cells. (wikipedia.org)
  • progression
  • On these bases, we investigated the mechanistic connection of H(2)O(2) yield, mitochondrial activation of Akt1 and cell cycle progression in NIH/3T3 cell line with confocal microscopy, in vivo imaging, and directed mutagenesis. (jove.com)
  • epithelial
  • BAFF is secreted by a variety of cells: monocytes and macrophages, bone marrow stromal cells, astrocytes, synoviocytes during rheumatoid arthritis, salivary epithelial cells during Sjögren's syndrome, astrocytes in certain glioblastomas. (wikipedia.org)
  • receptors
  • Although paracrine signaling elicits a diverse array of responses in the induced cells, most paracrine factors utilize a relatively streamlined set of receptors and pathways. (wikipedia.org)
  • synthesis
  • Here, we have reported the ability of the sphingomyelin present in the nuclear microdomain to bind DNA and regulate its synthesis, and to highlight its role in cell proliferation induced by partial hepatectomy. (mdpi.com)
  • During the S-phase of the cell cycle, the stimulation of sphingomyelinase and inhibition of sphingomyelin-synthase are accompanied by the DNA synthesis start. (mdpi.com)
  • fetal and neonatal cardiac myocytes are capable of undergoing DNA synthesis and cell division. (physiology.org)
  • Behavior of Cajal bodies and nucleoli after interference with inhibitors of RNA synthesis and its association with cell lines in culture. (usp.br)
  • stem
  • Brain tumors have been suggested to possess a small population of stem cells that are the root cause of tumorigenesis. (jove.com)
  • Neurosphere assays have been generally adopted to study the nature of neural stem cells, including those derived from normal and tumorous tissues. (jove.com)
  • Potential therapies could include stimulating the myocytes to divide and adding new cells via pharmacological and genetic manipulations or delivering stem cells to multiply and subsequently differentiate into cardiomyocytes ( 7 ). (physiology.org)
  • However, its role in proliferation of cardiomyocyte derived from NPY-pretreated mesenchymal stem cells (MSC) and its therapeutic potential in stem cell-mediated therapy for MI has not been explored to date. (physiology.org)
  • STK3 /MST2 and STK4 / MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. (rcsb.org)
  • Ligands that bind to RTKs include fibroblast growth factors, epidermal growth factors, platelet-derived growth factors, and stem cell factor. (wikipedia.org)
  • lymphocytes
  • It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. (wikipedia.org)
  • mitotic
  • Histologically, uveal melanomas with epithelioid morphology fare worse than those with spindle cells ( 7 ), as do those with higher mitotic activity, extrascleral invasion, or the presence of microvascular networks ( 3 , 8 , 9 ). (aacrjournals.org)
  • adult
  • Neuropeptide Y (NPY) induced reentry of differentiated rat neonatal and adult cardiomyocytes into the cell cycle. (physiology.org)
  • NPY directly induced neonatal and adult cardiomyocyte cell-cycle reentry and enhanced the number of differentiated cardiomyocytes from MSC in the infarcted myocardium, which corresponded to improved cardiac function, reduced fibrosis, ventricular remodeling, and increased angiomyogenesis. (physiology.org)
  • Adult mammalian cardiomyocytes are considered terminally differentiated, incapable of proliferation and irreversibly withdrawn from the cell cycle soon after birth ( 28 , 29 ). (physiology.org)
  • Although the adult heart has a limited capacity for myocyte proliferation, increasing the number of remaining cardiomyocytes by activating their proliferative potential via extrinsic factors could result in the repair of damaged myocardium. (physiology.org)
  • Here, we have investigated whether NPY can trigger cell-cycle reentry in neonatal or adult cardiomyocytes and increase the proliferation of transplanted MSC that have already differentiated into cardiomyocytes within the infarcted myocardium. (physiology.org)
  • Vivo-Morpholinos, in which the oligo is covalently linked to a delivery dendrimer, enter cells when administered systemically in adult animals. (wikipedia.org)
  • Signaling
  • The integrin-mediated and syndecan-4-mediated components of fibronectin-dependent signaling can be separated by stimulating cells with recombinant fibronectin fragments. (jove.com)
  • Paracrine signaling of growth factors between nearby cells has been shown to exacerbate carcinogenesis. (wikipedia.org)
  • Mice
  • Mice generated from these ES cells were viable, suggesting that PTPkappa phosphatase activity is not necessary for embryonic development. (wikipedia.org)
  • Therefore
  • If the transgene was incorporated into a gene with a signal sequence, β-gal activity would remain in the cytosol of the cell and therefore be active. (wikipedia.org)
  • The proliferating cells are in the ventricular zone in the neural tube and therefore colored red. (wikipedia.org)
  • fate
  • These results indicate that Akt1 intramitochondrial-cycling is central for redox modulation of cell fate. (jove.com)