Nuclease. Medical search. Frequent questions

S1 nuclease is used in the laboratory as a reagent in nuclease protection assays. (rockland.com)

The model with DNA suggests that the beta-hairpin partly blocks the active site, and in vivo activity assays show that the nuclease domain is not functional in the absence of the ATPase domain. (rcsb.org)

Some nucleases involved in DNA repair exhibit partial sequence-specificity. (wikipedia.org)
BisMapR confers strand specificity to nuclease-based genome-wide R-loop detection. (elifesciences.org)

Nuclease-free Water is ideal for the preparation of reagents and for use in enzymatic reactions. (neb.com)
The first of the targetable cleavage reagents to be developed were the zinc-finger nucleases (ZFNs) (1), which consist of a non-specific cleavage domain from a restriction endonuclease linked to a DNArecognition domain made up of zinc fingers. (ddw-online.com)
These in-house formulated reagents are fluorescence-quenched oligonucleotide probes that emit a fluorescent signal only after nuclease degradation. (idtdna.com)
Qualitative measurements are useful for testing lab reagents, equipment, and supplies for nuclease contamination. (idtdna.com)

Clustered, regularly interspaced, short palindromic repeat (CRISPR) RNA-guided nucleases (RGNs) have rapidly emerged as a facile and efficient platform for genome editing. (nature.com)
Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. (nature.com)
MAD7 is an engineered nuclease of the Class 2 kind V-A CRISPR-Cas (Cas12a/Cpf1) household with a low stage of homology to canonical Cas12a nucleases. (trancheproject.org)
Aldevron CRISPR nucleases are designed for seamless transition to the clinic. (idtdna.com)
The Spy Fi ® Cas9 Nuclease is designed for greater editing efficiency and reduced off-target effects without loss of on-target activity to help accelerate your CRISPR-Cas9 gene editing program. (idtdna.com)
Eureca-V is categorized as a Class 2, Type V CRISPR-Cas nuclease. (idtdna.com)
With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. (vectorbiolabs.com)
Modification of existing genes in living animal and human cells is enabled by engineered nucleases such as meganucleases, zinc finger nucleases, transcription activator-like effector-based nucleases, and the CRISPR-Cas system. (cdc.gov)

For example, EcoRI comes from Escherichia coli RY13 bacteria, while HindII comes from Haemophilus influenzae strain Rd. Numbers following the nuclease names indicate the order in which the enzymes were isolated from single strains of bacteria: EcoRI, EcoRII. (wikipedia.org)
These nucleases provide a one-vendor strategy for preclinical research through GMP gene editing enzymes, engineered to be used in a variety of applications including electroporation, transfection and microinjection. (idtdna.com)
Several of these genes, the TREX1 , RNASEH2A , RNASEH2B , and RNASEH2C genes, provide instructions for making nucleases, which are enzymes that help break down molecules of DNA and its chemical cousin RNA when they are no longer needed. (medlineplus.gov)

Knockout rats via embryo microinjection of zinc-finger nucleases. (mcw.edu)
Efficient targeted mutagenesis in the monarch butterfly using zinc-finger nucleases. (umassmed.edu)
Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. (uky.edu)

The DNA-packaging motor in tailed bacteriophages requires nuclease activity to ensure that the genome is packaged correctly. (rcsb.org)
Regulation of Nucleosome Architecture and Factor Binding Revealed by Nuclease Footprinting of the ESC Genome. (umassmed.edu)

The folding of staphylococcal nuclease (SNase) is known to proceed via a major intermediate in which the central OB subdomain is folded and the C-terminal helical subdomain is disordered. (pasteur.fr)
Mobile Unnatural Amino Acid Side Chains in the Core of Staphylococcal Nuclease. (atomistry.com)
Here we demonstrate that Tudor staphylococcal nuclease (SND1 or TSN) is overexpressed in NSCLC cell lines and tissues, and is important for maintaining NSCLC chemoresistance. (oncotarget.com)
To examine the role of water penetration, we have measured the 17O and 2H magnetic relaxation dis- persions (MRD) for the V66E and V66K mutants of staphylococcal nuclease, where glutamic acid and lysine residues are buried in predominantly apolar environments. (lu.se)

The Worthington product is useful as a stabilizing agent in reactions and dilutions and as a ballast protein in precipitations where contaminating nucleases and proteases are a concern. (worthington-biochem.com)
Herein we report the application of oxidative artificial chemical nucleases as novel agents for protein engineering. (rsc.org)
saCas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. (mbpinc.net)
With great tolerance to various inhibitors of proteases used in protein purification, Dr. Nuclease reduces cell clumping in seconds, therefore improves the protein purification process and yield. (sydlabs.com)
Random mutagenesis of the low-G + C portion encoding PrrC residues 200-313 was performed, followed by selection for loss of anticodon nuclease-dependent lethality and production of full-sized PrrC-like protein. (tau.ac.il)
Techniques for measuring specific nucleic acid interaction with another nucleic acid or with a protein by digestion of the non-interacting nucleic acid by various nucleases. (bvsalud.org)

SpCas9 Nuclease and SpyFi Cas9 Nuclease are available for immediate delivery at cGMP quality. (idtdna.com)
When used in combination with effective guide, target site, and double-stranded DNA cut strategies, Spy Fi Cas9 Nuclease can deliver comparable on-target editing and less off-target activity than wild-type SpCas9 nuclease. (idtdna.com)
The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. (mbpinc.net)
The Cas9 nuclease from the bacteria Staphylococcus aureus , abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. (mbpinc.net)

Linking an array of such modules to the same cleavage domain used in ZFNs led to the development of TALE nucleases, or TALENs. (ddw-online.com)

Nuclease primary structure is by and large poorly conserved and minimally conserved at active sites, the surfaces of which primarily comprise acidic and basic amino acid residues. (wikipedia.org)
Such a nuclease diffuses along DNA until it encounters a target, upon which the residues of its active site interact with the chemical groups of the DNA. (wikipedia.org)
Here, we propose that the nuclease activity is regulated by movement of the beta-hairpin, altering active site access and the orientation of catalytically essential residues. (rcsb.org)

An important development came when H.O. Smith, K.W. Wilcox, and T.J. Kelly, working at Johns Hopkins University in 1968, isolated and characterized the first restriction nuclease whose functioning depended on a specific DNA nucleotide sequence. (wikipedia.org)
These targetable nucleases allow creation of new mutations at specific sites, introduction of designed sequence changes and production of larger alterations, as desired by the experimenter. (ddw-online.com)

This nuclease-free water is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity. (thermofisher.com)
In addition to the application of developmental metallodrugs as inducers of chemical nuclease activity, the effects of cytotoxic trinuclear platinum(II) complexes as high-affinity DNA binders that inhibit-or block-endonuclease enzyme recognition and excision are reported through a wide variety of biophysical and molecular biological methods. (dcu.ie)
S1 nuclease is an endonuclease that is active against single-stranded DNA and RNA molecules. (rockland.com)
Similar to MilliporeSigma Benzonase nuclease, a genetically engineered endonuclease from Serratia marcescens, Syd Labs recombinant Dr. Nuclease degrades all forms of DNA and RNA while having no proteolytic activity. (sydlabs.com)
Dr. Nuclease is a recombinant endonuclease produced in E. coli. (sydlabs.com)
Dr. Nuclease acts as an endonuclease that degrades both DNA and RNA in single-stranded, double-stranded, linear, circular, and super-coiled forms. (sydlabs.com)

This wild-type spCas9 nuclease is available for use in development work as well as standard GMP products for clinical studies. (idtdna.com)
This is an AAV-DJ expressing spCas9 nuclease under a short CMV7 promoter. (vectorbiolabs.com)

A nuclease must associate with a nucleic acid before it can cleave the molecule. (wikipedia.org)
No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems. (biomedcentral.com)
After all non-interacting regions are eliminated by nuclease digestion, the protected nucleic acid that remains is analyzed. (bvsalud.org)

Sharipova M.R, Balaban N.P, Mardanova A.M, Effect of nucleases on bacteria infected with bacteriophages//Biology Bulletin. (kpfu.ru)

Synthesizing oligonucleotides that contain 2'-OMe modifications can prevent degradation by nucleases while also increasing binding affinity for its target, making it particularly useful in antisense oligonucleotides. (biosearchtech.com)

This family belongs to a superfamily of PDDEXK nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI. (nih.gov)

Nucleases are also extensively used in molecular cloning. (wikipedia.org)
Most nucleases are classified by the Enzyme Commission number of the "Nomenclature Committee of the International Union of Biochemistry and Molecular Biology" as hydrolases (EC-number 3). (wikipedia.org)
The chemical nuclease of [Cu(Phen)2]2+ (where Phen = 1,10-phenanthroline), is a well studied reagent which randomly cleaves nucleic acids in the presence of molecular oxygen (or hydrogen peroxide) upon reduction to Cu+. (dcu.ie)

The nuclease assay consisted of incubating the OSS with the respective DNA and running on a polyacrylamide, in the case of linear DNA, and an agarose gel in the case of plasmid DNA. (biomedcentral.com)
Genotyping was performed using a 5'-nuclease polymerase chain reaction assay. (cdc.gov)

We show that SeAgo is a DNA-guided nuclease preferentially acting on single-stranded DNA targets, with non-specific guide-independent activity observed for double-stranded substrates. (nih.gov)
Linear, single- and double-stranded, and plasmid DNA substrates were tested for nuclease contamination in oxygen scavenging systems that are routinely used during single-molecule imaging. (biomedcentral.com)
Two oligonucleotide substrates are offered for nuclease detection, RNaseAlert ® and DNaseAlert™ substrates. (idtdna.com)

The tRNA(Lys)-specific anticodon nuclease exists in latent form in Escheri chia coli strains containing the optional prr locus. (tau.ac.il)

Nucleases variously effect single and double stranded breaks in their target molecules. (wikipedia.org)
Double-stranded circular pUC19 plasmid DNA (2686 bp) was tested for potential nuclease contamination. (biomedcentral.com)

Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. (biomedcentral.com)
This study evaluates the potential for nuclease contamination in these oxygen scavenging systems. (biomedcentral.com)
Using size-exclusion chromatography, this study reported the presence of nuclease contamination in the 40- and 100 kDa elution fractions of routinely used OSS system with protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD), with the 40 kDa fraction exhibiting the highest nuclease activity. (biomedcentral.com)
Analysis for potential nuclease contamination in commonly used single-molecule oxygen scavenging systems. (biomedcentral.com)
Use Nuclease Decontamination Solution on and around your workspace to irreversibly inactivate nucleases and reduce contamination risk. (idtdna.com)

Site-specific gene modification and highly specific in vitro cutting is enabled by nucleases that can be easily programmed with nucleic acids. (neb.com)
The most commonly used modification for nuclease resistance is 2'-OMe, which naturally occurs on small RNAs. (biosearchtech.com)
The latency is a result of a masking interaction between the anticodon nuclease core-polypeptide PrrC and the Type IC DNA restriction-modification enzyme EcoprrI. (tau.ac.il)

Mutations in any of these genes are believed to result the absence or abnormal functioning of the respective nuclease enzyme. (medlineplus.gov)

Prisecaru, Andreea (2015) Metallodrugs as inducers and inhibitors of chemical nuclease activity. (dcu.ie)

Nucleases variously employ both nonspecific and specific associations in their modes of recognition and binding. (wikipedia.org)

You can limit or mitigate this susceptibility, which is particularly important for antisense or RNAi applications, by incorporating nuclease-resistant modifications into oligonucleotides during synthesis. (biosearchtech.com)

Griffiths, AD , Potter, BVL & Eperon, IC 1987, ' Stereospecificity of nucleases towards phosphorothioate-substituted RNA: stereochemistry of transcription by T7 RNA polymerase ', Nucleic Acids Research , vol. 15, no. 10, pp. 4145-4162. (bath.ac.uk)

This nuclease activity is tightly regulated as the enzyme is inactive for the duration of DNA translocation. (rcsb.org)
Any process that modulates the frequency, rate or extent of nuclease activity, the hydrolysis of ester linkages within nucleic acids. (ntu.edu.sg)
Activity similar to MilliporeSigma Benzonase nuclease. (sydlabs.com)

Dr. Nuclease eliminates DNA/RNA containments in various products with ultra-speed and efficacy. (sydlabs.com)

One unit of Dr. Nuclease corresponds approximately to the required amount of enzyme to digest 37 μg of DNA in the reaction buffer, 50 mM Tris-HCl, pH 8.0, 1 mM MgCl2, for 30 minutes at 37°C. (sydlabs.com)

There are several other oligonucleotide modifications you can use to introduce nuclease resistance. (biosearchtech.com)

Taken together, the data suggest that the anticodon of tRNA(Lys) harbours anticodon nuclease identity elements and implicates a conserved region in PrrC in their recognition. (tau.ac.il)

For clients applying gene editing to investigate and treat genetic and inherited diseases, these nucleases provide an accelerated path to the clinic by offering the same product manufactured at research grade and full cGMP, supported by the quality documentation necessary for regulatory filings. (idtdna.com)

Nucleases are widely present in research labs and can interfere with experiments that use nucleic acids. (idtdna.com)

Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. (thermofisher.com)
It has been publicly launched as a royalty-free nuclease for each tutorial and business use. (trancheproject.org)

Anatomy9

Organisms25

Chemicals and Drugs121

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes82

Technology, Industry, Agriculture1

Information Science3

Health Care3

Protection assays1

Assays1

Specificity2

Reagents4

CRISPR8

Enzymes3

Zinc3

Genome2

Staphylococcal4

Protein6

Cas9 Nuclease4

ZFNs1

Residues3

Sequence2

Endonuclease6

SpCas92

Nucleic acid3

Bacteriophages1

Degradation1

Superfamily1

Molecular3

Assay2

Substrates3

Coli1

Double-stranded2

Contamination5

Modification3

Mutations1

Inhibitors1

Nonspecific1

Oligonucleotides1

Transcription1

Activity3

Eliminates1

Reaction1

Resistance1

Recognition1

Grade1

Labs1

Free2