• nucleus
  • Immunofluorescence staining studies showed that upon doxorubicin treatment, the wild-type FOXO3a relocalized to the nucleus, whereas the phosphorylation-defective FOXO3a (Ala-7) mutant remained largely in the cytoplasm. (jove.com)
  • Similar to other nuclear receptors, when activated, FXR translocates to the cell nucleus, forms a dimer (in this case a heterodimer with RXR) and binds to hormone response elements on DNA, which up- or down-regulates the expression of certain genes. (wikipedia.org)
  • Inside those cells, it can be located in the nucleus and mitochondrion since its main function is related with DNA polyadenilation and these cell organells are the only ones were DNA can be found. (wikipedia.org)
  • HIPK2 is found in the nucleus within structures called nuclear speckles. (wikipedia.org)
  • gene
  • In the present study, we show that sodium butyrate treatment of human breast cancer MCF-7 cells transcriptionally activates the GalNAc-T3 gene. (biochemj.org)
  • Transient transfection of plasmids containing a reporter gene under the control of GalNAc-T3 indicated that several transcriptional elements are involved in response to sodium butyrate, with the nuclear respiratory factor-1 (NRF-1)-binding motif located between −88 and −77nt being the most important. (biochemj.org)
  • Here we discuss PTEN ubiquitination, PTEN complex formation with components of the adherens junction, PTEN nuclear localization, and microRNA regulation of PTEN as essential regulatory mechanisms that determine PTEN function independent of gene mutations and epigenetic events. (hindawi.com)
  • Another group identified the same gene in the search for new dual-specific phosphatases and named it TEP-1 (TGF- β regulated and epithelial cell-enriched phosphatase) [ 3 ]. (hindawi.com)
  • MDA-MB-435 and PC3 cells, resistant to DF 203, did not show drug-induced CYP1A1 and CYP1B1 gene expression. (aspetjournals.org)
  • More specifically, ligand binding to a nuclear receptor results in a conformational change in the receptor, which, in turn, activates the receptor, resulting in up- or down-regulation of gene expression. (wikipedia.org)
  • The bile acid receptor (BAR), also known as farnesoid X receptor (FXR) or NR1H4 (nuclear receptor subfamily 1, group H, member 4) is a nuclear receptor that is encoded by the NR1H4 gene in humans. (wikipedia.org)
  • In 2000, the location of the HIPK2 gene was discovered to be on the long arm of Chromosome 7 (human) in the human genome. (wikipedia.org)
  • Drosophila
  • While Drosophila AKT and its upstream regulators, such as PI3K and PDK1, are essential for viability and cell growth, flies lacking TORC2 are viable and display only minor growth impairment under standard growth conditions. (beds.ac.uk)
  • tissues
  • This bioassay that measures estrogen-induced increase of the number of human breast cancer cell, which is biologically equivalent to the increase of mitotic activity in tissues of the genital tract, was originally developed by Soto et al. (wikipedia.org)
  • suppression
  • One of the primary functions of FXR activation is the suppression of cholesterol 7 alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis from cholesterol. (wikipedia.org)
  • kinase
  • Doxorubicin treatment of MCF-7 breast carcinoma cells results in FOXO3a nuclear relocation and the induction of the stress-activated kinase p38 MAPK. (jove.com)
  • AKT kinase activity is principally determined by the level of phosphatidylinositol-3,4,5-triphosphate (PIP3) in the plasma membrane of cells, which is generated by phosphatidylinositol-3-kinase (PI3K) upon stimulation of receptor tyrosine kinases. (beds.ac.uk)
  • For AKT kinase assays, cells were treated with Insulin (Sigma, 10 μg/ml) and wortmannin (Sigma, 50 nM) for 20 min. (beds.ac.uk)
  • In addition to its kinase domain, HIPK2 has two nuclear localization signals, a SUMO interaction motif, an auto-inhibitory domain a transcriptional co-repression domain, and several interaction domains, including one for p53. (wikipedia.org)
  • phosphorylation
  • Phosphorylation of FOXO3a on Ser-7 by P38 Promotes Its Nuclear Localization in Response to Doxorubicin The Journal of Biological Chemistry. (jove.com)
  • HPLC-coupled phosphopeptide mapping and mass spectrometric analyses identified serine 7 as a major site for p38 phosphorylation. (jove.com)
  • Together, our results suggest that p38 phosphorylation of FOXO3a on Ser-7 is essential for its nuclear relocalization in response to doxorubicin. (jove.com)
  • pathway
  • Extract from the American groundnut was shown to not have anti-oxidative activity itself, but to drive the anti-oxidative pathway in cells. (wikipedia.org)
  • survival
  • In recent years, an increasing number of clinical studies have suggested that an optimal vitamin D status has a protective effect against BC development and that high Vitamin D receptor (VDR) expression in breast tumors is associated with a better survival rate [ 5 , 6 , 7 , 8 ]. (mdpi.com)
  • Biology
  • Were not sure if entosis evolved to play a particular role or if its simply an aberration of a normal process, adds Brugge, who is Chair of the Department of Cell Biology. (bio-medicine.org)
  • In collaboration with Guillaume Normand of Associate Professor of Cell Biology Randall Kings lab, he tracked the cells over time and was shocked to see that some of them actively bored into their neighbors. (bio-medicine.org)
  • To receive news and publication updates for International Journal of Cell Biology, enter your email address in the box below. (hindawi.com)
  • Nuclear Medicine and Biology , 27 , 643-646. (springer.com)
  • International Institute of Molecular and Cell Biology in Warsaw, ul. (nih.gov)
  • DNA and Cell Biology. (wikipedia.org)
  • stromal cells
  • Both ERs are widely expressed in different tissue types, however there are some notable differences in their expression patterns: The ERα is found in endometrium, breast cancer cells, ovarian stromal cells, and the hypothalamus. (wikipedia.org)
  • In a study regarding multiple myeloma bone marrow mesenchymal stromal cells shows that the expression of C8orf48 is lower in the disease state cells in comparison to healthy cells. (wikipedia.org)
  • inhibit
  • Recently, as part of a new therapeutic strategy for breast cancers, several groups of compounds (1 , 2 , 3 , 4 , 5 , 6 , 7 , 8) have been designed to inhibit estrone sulfatase. (aacrjournals.org)
  • hormone
  • In each experiment, the cells are exposed to a dilution series of 17β-estradiol (0.1 pM-1000 pM) for providing a positive control (standard dose-response curve), and treated only with hormone-free medium as a negative control. (wikipedia.org)
  • thus
  • The cytoplasm-predominant CS mutation ( PTEN K289E ) has been reported to disrupt PTEN ubiquitination and thus abolish its nuclear import ( 8 ). (pubmedcentralcanada.ca)
  • sodium
  • The nuclear proteins were extracted utilizing a commercially manufactured kit and separated on two dimensional (2-D) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). (edu.pl)
  • Nuclear content of NRF-1 remained constant in MCF-7 cells treated with or without sodium butyrate. (biochemj.org)
  • regulation
  • PTEN contributes to cell cycle regulation by blocking cells entering the S-phase of the cell cycle and by upregulation of p27kip1, which is recruited into the cyclin E/cdk2 complex [ 17 ], and downregulation of cyclin D1 [ 18 ]. (hindawi.com)
  • These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. (wikipedia.org)
  • cancer
  • She informed him that the scientific literature is full of cell-in-cell references in the context of cancer. (bio-medicine.org)
  • For example deregulation of Akt, ras and MYC as well as loss of p53 function have been reported to confer increased glucose metabolic rates in cancer cells. (springer.com)
  • 2003). Positron emission tomography in non-small-cell lung cancer: prediction of response to chemotherapy by quantitative assessment of glucose use. (springer.com)
  • High levels of CARD8 expression were found in tumor cell lines, including breast , prostate , ovarian and colon cancer cells as well as high expression in non-small cell lung cancer (NSCLC) cells and with hardly detectable expression in normal lung, in contrast to a lack of expression in small-cell lung cancer cell lines. (atlasgeneticsoncology.org)
  • In tumor specimens from patients CARD8 expression has been demonstrated in colon cancer and non-small cell lung cancer. (atlasgeneticsoncology.org)
  • For example a number of breast cancer cell lines do not express TUCAN/CARD8-54, although some of them express TUCAN/CARD8-48. (atlasgeneticsoncology.org)
  • Rottlerin suppresses growth of human pancreatic tumors in nude mice, and pancreatic cancer cells isolated from Kras(G12D) mice," Cancer Letters , vol. 353, no. 1, pp. 32-40, 2014. (hindawi.com)
  • In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy Ki-67 is an excellent marker to determine the growth fraction of a given cell population. (wikipedia.org)
  • It is also present, together with ERα in some human breast cancer cell lines. (wikipedia.org)