Normally cells cell proliferation. Medical search. Frequent questions

In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. (mdpi.com)
Dnmt3a regulates both proliferation and differentiation of mouse neural stem cells. (ca.gov)
DNA methylation is known to regulate cell differentiation and neuronal function in vivo. (ca.gov)
Here we examined whether deficiency of a de novo DNA methyltransferase, Dnmt3a, affects in vitro differentiation of mouse embryonic stem cells (mESCs) to neuronal and glial cell lineages. (ca.gov)
Early-passage neural stem cells (NSCs) derived from Dnmt3a-deficient ESCs exhibited a moderate phenotype in precocious glial differentiation compared with wild-type counterparts. (ca.gov)
Thus, our work revealed a novel role for Dnmt3a in regulating both the timing of neural cell differentiation and the cell proliferation in the paradigm of mESC-derived-NSCs. (ca.gov)
The EPCs plays critical role in postnatal vasculogenesis through pivotal bioactivities of mobilization, homing, migration, differentiation, and proliferation in angiovasculogenic tissues [ 6 ]. (hindawi.com)
The differentiation of endometrial stromal cells into decidual cells, termed decidualization, is an integral step in the establishment of pregnancy. (bioone.org)
Gene ontology and upstream regulator pathway analysis showed that WNK1 regulates cell migration, differentiation, and proliferation. (bioone.org)
The SRY-box 4 (SOX4) gene, which is located on chromosome 6p22.3, encodes a 47-kDa protein that is a member of the sex-determining region Y-related high-mobility group-box transcription factor family and has functions in embryonic development and cell differentiation ( 3 , 4 ). (spandidos-publications.com)
miRNAs participate in crucial biological processes, including development, differentiation and proliferation ( 15 , 16 ). (spandidos-publications.com)
Even though its sequence along with its expressional pattern in cancer cell lines are evident, the functional aspects concerning cell proliferation, viability, differentiation and cell cycle regulation of NDRG1 remains vague. (scirp.org)
These results revealed that NDRG1 is functional in prostate cancer cells and able to induce expression of differentiation factors through p53 independent pathway. (scirp.org)
T. Napso, N. Azzam, A. Lerner and F. Fares, " N-myc Downstream Regulated Gene 1 Increases Differentiation Factors Level in Human Prostate Cancer Cells without Affecting Cell Proliferation and Cell Cycle Profiles," Journal of Cancer Therapy , Vol. 4 No. 2, 2013, pp. 568-574. (scirp.org)
A Novel Gene Which Is Up-Regulated during Colon Epithelial Cell Differentiation and Down-Regulated in Colorectal Neoplasms," Laboratory Investigation: A Journal of Technical Methods and Pathology, Vol. 77, No. 1, 1997, pp. 85-92. (scirp.org)
The hallmark of embryonic stem (ES) cells is their ability for self-renewal (capability of unlimited cell division without the loss of pluripotency) as well as for differentiation into all cell types of the adult organism. (uni-muenchen.de)
A high expression of Pes1, Bop1 and WDR12 was observed in ES cells, which strongly decreased during in vitro differentiation. (uni-muenchen.de)
Enhancer of zeste homolog 2 (EZH2) belongs to poly-sulfur protein family and can mediate cell proliferation and differentiation via the modulation of various genes expressions. (medscimonit.com)
Here we analyzed the genome-wide changes in gene transcription occurring during growth and differentiation of porcine primary myoblast cells. (au.dk)
While substantial progress has been made to delineate the developmental pathway of T-cell lineage commitment, still little is known about the relationship between differentiation competence and the remarkable expansion of the earliest (DN1 stage) T-cell progenitors. (lu.se)
To satisfy differentiation kinetics and overall cell yield data, our models require that adult DN1 cells divide multiple times before becoming competent to progress into DN2 stage. (lu.se)
it has been hypothesized that dietary calcium may increase prostate cancer risk by reducing circulating levels of 1,25-dihydroxyvitamin D (1,25[OH] 2 D) (10), which promotes the differentiation and inhibits the proliferation of prostate cells (11). (cdc.gov)

Therefore, this study proves that Rh4 inhibits cancer cell proliferation by activating the ROS/p53 signaling pathway and activating autophagy to induce ferroptosis, which provides necessary scientific evidence of the great anticancer potential of Rh4. (hindawi.com)
The DEmRNAs were mainly enriched in tumorigenesis‑associated GO terms (angiogenesis, positive regulation of cell proliferation, positive regulation of fibroblast apoptotic processes and regulation of neutrophil migration) and pathways (Hippo signaling pathway, cancer pathways, and Wnt signaling pathway). (spandidos-publications.com)
Besides, the results suggested that FBXO17 may inhibit the Wnt/β-catenin signaling pathway and influence the expression of adhesion molecules, such as E-cadherin and N-cadherin in Ishikawa cells. (medsci.org)
si-Prok1 arrests cell cycle, induces apoptotic changes, and inhibits the proliferation of ovarian granulosa cells through a mechanism related to the regulation of Pi3k/Akt signaling pathway. (ajol.info)
On the other hand, in order to identify miR-BARTs that can activate the Wnt signaling pathway, I analyzed the PAR-CLIP data set of C666 cells and discovered nine anti-Wnt signaling targets of miR-BARTs, including seven novel genes and two pro-apoptotic genes identified above. (duke.edu)

We demonstrate that UCN-01, at concentrations lower than those necessary to inhibit cancer cell growth, inhibit proliferation of human endothelial cells in vitro. (karger.com)

Cell proliferation, cell counting, colony formation assay, and Transwell assay were applied to evaluate the proliferation and migration of breast cancer cells. (medscimonit.com)
Functional analysis revealed that knockdown of PTGR1 significantly inhibited proliferation and colony formation by PC cells. (go.jp)
Our results showed that beginning at four months, nFe2O3-exposed Beas2B underwent neoplastic-like transformation, as indicated by increased cell proliferation and attachment-independent colony formation. (cdc.gov)

We conclude that UCN-01, at clinically relevant concentrations, exerts an anti-neovascularization effect by blocking two important steps in vessel formation: (1) the response of cancer cells to hypoxia, and (2) endothelial cell proliferation. (karger.com)

The inhibition of cell proliferation was related to arrest of the cell cycle in the G0/G1 phase and increased apoptosis in response to PTGR1 knockdown as indicated by flow cytometry. (go.jp)

Reduced hippocampal cell proliferation has been previously reported in schizophrenia, which may contribute to hippocampal dysfunction. (brainresearchlab.com)
Conclusion: Our results confirm that reduced hippocampal cell proliferation may be present in schizophrenia. (brainresearchlab.com)

The aim of the current study is to observe the effect of CSE on biological behavior of EPCs and, further, to search for potential candidate agent in protection of proliferation of EPCs against the damage caused by CSE exposure in vitro . (hindawi.com)
The 5-AZA-CdR partly protected the proliferation of EPCs against the damage caused by CSE exposure in vitro , suggesting that DNA methylation may be involved in the dysfunction of EPCs induced by CSE. (hindawi.com)
The method of stimulating isolated cells with CSE in vitro has been explored and frequently applied to determine the direct causes in the relationships between cigarette smoking and cellular functions [ 15 ]. (hindawi.com)
In this study, in attempt to provide fresh information about the impact of CS on proliferation of EPCs to elucidate the pathophysiological mechanisms of the diseases related to CS in cellular level, we assessed the proliferation of EPCs after interfering the cells with a series of concentrations of CSE for various times of exposure in vitro . (hindawi.com)
Primary human endometrial stromal cells (HESCs) were subjected to small interfering RNA knockdown of WNK1 followed by in vitro decidualization. (bioone.org)
In the present study, it was shown that FBXO17, which is a member of the F-box family, is abnormally downregulated in UCEC tissues compared with non-tumor endometrial tissues, and this was significantly associated with the clinical histological grade, as well as the abnormal proliferation of the UCEC cell line, Ishikawa, both in vitro and in vivo . (medsci.org)
In addition, knockdown of miR‑320 in breast cancer cell lines promoted cell proliferation and invasion in vitro, whereas miR‑320 overexpression had the opposite effect. (spandidos-publications.com)
Altogether our results corroborate the hypothesis of an inhibitory effect of metformin on PHEO cellular proliferation in vitro . (endocrine-abstracts.org)

2009). Evidence of similarities in the redox control of cell proliferation in animals and plants are provided, particularly glutathione recruitment into the nucleus early in the cell proliferation, which appears to be important in the control of progression through the cell cycle in both kingdoms. (cam.ac.uk)
Defects in the control of cell proliferation are a hallmark of cancer, and DNA replication is a key process for cell proliferation. (aacrjournals.org)
Mesenchymal cells were isolated from periodontal ligament of impacted third molars (n=2) and cultured on titanium disks (polished and nitrided) and on a plastic surface as a positive control of cell proliferation. (bvsalud.org)

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and cell proliferation assay were used to explore the functional role of the key lncRNAs in GBM. (spandidos-publications.com)
A widely used assay for staining dead cells is Trypan Blue. (dojindo.com)
Among the enzyme-based assays, the MTT assay is the best known for determining mitochondrial dehydrogenase activities in living cells. (dojindo.com)
Although the 3[H]thymidine-uptake assay is regarded as a reliable cell viability assay, the use of a radioisotope causes various concerns. (dojindo.com)
Therefore, CCK-8 is stable for at least 6 months at room temperature and one year at 0-5°C. Since WST-8, WST-8 formazan, and 1-Methoxy PMS have no cytotoxicity in cell culture media, additional experiments may be carried out using the same assay plate. (dojindo.com)
The CCK-8 assay involves most of the dehydrogenase in a cell, while MTT only involves mitochondrial dehydrogenase. (dojindo.com)
Therefore, the MTT assay depends on mitochondrial activity, not the cell itself. (dojindo.com)
Cell Counting Kit-F is another cell viability assay kit based on the esterase activity of viable cells. (dojindo.com)
These esterase substrates, therefore, can serve as viability assay probes for cells. (dojindo.com)
Cell proliferation, cell cycle distribution, and apoptosis were evaluated by the CCK-8 assay and flow cytometry using PI/Annexin V staining, respectively. (medsci.org)
Furthermore, a Dual‑Luciferase reporter assay indicated that SRY‑box 4 (SOX4) is a direct target of miR‑320, and the restoration of SOX4 in miR‑320‑overexpressing cells attenuated the tumor‑suppressive effects of miR‑320. (spandidos-publications.com)
Clonal formation assay analyzed the change of cell proliferation potency. (medscimonit.com)
The effect on tumorigenesis potency of glioma stem cells was determined by mouse transplantation assay. (medscimonit.com)
We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
The fluorochrome-based TUNEL assay applicable for flow cytometry, combining the detection of DNA strand breaks with respect to the cell cycle-phase position, was originally developed by Gorczyca et al. (wikipedia.org)
However, for years there has been a debate about its accuracy, due to problems in the original assay which caused necrotic cells to be inappropriately labeled as apoptotic. (wikipedia.org)

it inhibits cell migration, reduces the ability to form colonies and induces apoptosis or programmed cell death through the modulation of other key genes (p53, caspase-3, c-PARP) and various apoptotic factors," says Maurizio Battino, coordinator of the research. (agenciasinc.es)
High expression of lncRNA H19 promotes cell migration and enhances angiogenesis ( 11 , 12 ). (spandidos-publications.com)
When lncHERG is highly expressed, cell multiplication, invasion and migration are inhibited in GBM ( 16 ). (spandidos-publications.com)
This study aimed to investigate the relationship between miR-506 and proliferation and migration of breast cancer cells. (medscimonit.com)

Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle through inhibiting apoptosis and promoting cell division (4,5). (cellsignal.com)

Each antibody in the SignalStain ® Proliferation/Apoptosis IHC Sampler Kit detects endogenous levels of its target protein and does not cross-react with any related proteins. (cellsignal.com)
Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (3). (cellsignal.com)
The relationship between cell proliferation, protein tyrosine phosphorylation, phosphotyrosine kinase activity and bradykinin receptor activation in rat mesangial cells was investigated. (nih.gov)
Transcriptional coactivator Yes-associated protein (YAP) can promote the proliferation of multiple cancer cells, corneal endothelial cells, and vascular smooth muscle cells, which participate in angiogenesis. (molvis.org)
This abrogated expression of the decidual marker genes, insulin like growth factor binding protein 1 ( IGFBP1 ) and prolactin ( PRL ), and prevented adoption of decidual cell morphology. (bioone.org)
UCN-01, a protein kinase C (PKC) inhibitor, induces growth arrest and apoptosis in cancer cells and was recently introduced in a phase I clinical trial. (karger.com)
Exogenous OGF was observed to have a dose-dependent, serum-independent, reversible, and receptor-mediated inhibitory action on cell proliferation that was dependent on RNA and protein synthesis. (nih.gov)
The proliferation of endothelial cells is detected by MTT method and adhesion of endothelial cells observed by PI fluorescence staining and western-bolt detection of protein of VE-cadherin. (atlantis-press.com)
Results: The proliferation of endothelial cells on the titanium surface is better than the culture plate surfaces, the adhesion cells on titanium surface are more numbers, cell morphology is more extended, titanium surface expression of VE-cadherin protein higher than the culture plate surface. (atlantis-press.com)
Cell cycle ratio and apoptosis in the two groups were determined using flow cytometry, while Pi3k/Akt signal route-linked protein levels were assayed by immunoblot method. (ajol.info)
In EBV latently infected epithelial cells, such as nasopharyngeal carcinoma (NPC) and gastric carcinoma (GaCa) cells, viral protein expression is low. (duke.edu)
p53 is a key protein that is altered in all cancer cells. (who.int)
Mechanistically, we found that U-73122 inhibited DES-induced activation of cAMP- response element binding protein (CREB) in gubernaculum testis cells . (bvsalud.org)
10%. In the remaining 10% of children with lecular weight protein found on the surface nephrotic syndrome, it is largely mediated of all nucleated cells which synthesize it. (who.int)

It also suppresses the receptor of the epidermal growth factor called EGFR/HER2 and its signalling pathways, which could be an attractive target in cancer therapies because of their important role in the processes of cell survival and proliferation, as well as in those of apoptosis and metastasis. (agenciasinc.es)

Conclusion: The SLA Ti surface can promote the proliferation and adhesion of endothelial cell and could promote the early healing process of osseointegration. (atlantis-press.com)

Among them, VEGF-A can promote the division and proliferation of vascular endothelial cells and neovascularization and maintain the survival of new vessels. (molvis.org)
During myogenesis the satellite cells undergo a series of highly ordered events that include division and proliferation of mononucleated myoblasts, irreversible cell cycle withdrawal, elongation of myoblasts and finally cell-cell fusion, leading to the formation of multinucleated myotubes. (au.dk)

Conditional knockout of α1A adrenergic receptors in OPCs suppresses spontaneous and locomotion-induced Ca 2+ increases and reduces OPC proliferation. (nature.com)
For example, lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) is highly expressed in GBM tissues and perturbed expression of NEAT1 suppresses cell proliferation and invasion ( 10 ). (spandidos-publications.com)
Anti-androgen flutamide suppresses hepatocellular carcinoma cell proliferation via the aryl hydrocarbon receptor mediated induction of transforming growth factor-β1. (oregonstate.edu)
however, it also suppresses cancer cell proliferation and recent findings have implicated its role as a tumor suppressor. (oregonstate.edu)

mitotically active cells of intact segments do not significantly contribute to the blastemal cellular sources. (mdpi.com)
The SignalStain ® Proliferation/Apoptosis IHC Sampler Kit from Cell Signaling Technology allows the researcher to examine paraffin-embedded tissues or cells with antibodies that will detect cellular apoptosis or proliferation. (cellsignal.com)
Cellular division for any cell type is dependent on the inherent function, location and the response of cells to repair, apoptosis, or death. (biolegend.com)
Immunofluorescent double staining was used to detect YAP cellular localization with CD31 (an endothelial cell marker) antibody. (molvis.org)
Cellular enzymes such as lactate dehydrogenase (LDH), adenylate kinase, and glucose-6-phosphate dehydrogenase are also used as cell death markers, and there are several such products available on the market. (dojindo.com)
Cellular redox homeostasis is considered to play an important role in the regulation of the plant cell cycle but there is little information on the precise functions of key redox metabolites such as ascorbate, glutathione and pyridine nucleotides in this process. (cam.ac.uk)
Results and Discussion: Metformin at 20 mM induced an inhibition of 60% of cell viability after 48 h treatment, as compared to untreated controls, and increased cellular lipid peroxidation, while decreased O 2 consumption in PC12-Adh PHEO cells. (endocrine-abstracts.org)
This effect is associated with enhanced lipid peroxidation and decreased O 2 consumption by those cells, which are both suggestive of increased cellular stress. (endocrine-abstracts.org)
Because of their surface charge, asbestos fibers can adsorb to cellular macromolecules (proteins, DNA, RNA) and cell surface proteins. (cdc.gov)
Some evidence suggests that dissolution of IONP following endocytosis into cells may disrupt iron homeostasis, resulting in genotoxicity and neoplastic-like cellular transformation. (cdc.gov)

While some assays utilize antibodies to study cell health, proliferation, cell cycle or apoptosis, other types of experiments can rely on non-antibody based methods of assessment, often called non-antibody chemical probes. (biolegend.com)
Methods: The human umbilical cord endothelial cells are cultured on the surface of SLA-Ti disks and culture plates. (atlantis-press.com)
Methods: Cell viability was evaluated by MTT and TRIPAN assays using the PC12-Adh PHEO cell line treated with metformin in increasing concentrations (0 30 mM). (endocrine-abstracts.org)
Since 1992 the TUNEL has become one of the main methods for detecting apoptotic programmed cell death. (wikipedia.org)

Learn about cell viability assays. (abcam.com)
Learn about the assays used to assess cell proliferation and the cell cycle. (abcam.com)
Cell viability and cytotoxicity assays are used for drug screening and cytotoxicity tests of chemicals. (dojindo.com)
only lactate dehydrogenase does not lose its activity during cell death assays. (dojindo.com)
Therefore, cell death assays based on LDH activity are more reliable than other enzyme-based cell death assays. (dojindo.com)
WSTs produce water-soluble formazans and are suitable for assays of cell proliferation and cytotoxicity. (dojindo.com)
Dehydrogenase-based assays reflect cell conditions with more sensitivity than the other assays because they depend on several elements including dehydrogenases, NAD(H), NADP(H), and mitochondrial activity. (dojindo.com)

The Ki-67 proliferation index and the apoptotic index using the terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling method were also evaluated. (aacrjournals.org)
To identify pro-apoptotic genes targeted by the five anti-apoptotic miRNAs, I validated one previously published target and identified nine novel targets by performing photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) in the EBV+ NPC cell line C666. (duke.edu)
There is evidence for C1q-mediated microglial phagocytosis in the clearance of apoptotic cells (36, 37). (nos-nop.org)
TUNEL is a method for detecting apoptotic DNA fragmentation, widely used to identify and quantify apoptotic cells, or to detect excessive DNA breakage in individual cells. (wikipedia.org)

The viability of GBM cells was significantly decreased following RNA interference with DLEU1 and TRAF4 production. (spandidos-publications.com)
In this method, cell viability must be determined by counting the unstained cells with a microscope or other instruments. (dojindo.com)
Fig. 1 indicates various reagents used for detection of cell viability. (dojindo.com)
Our results suggest that metformin has a moderate inhibitory effect on the viability of PC12-Adh PHEO cells. (endocrine-abstracts.org)

The results demonstrate that DLEU1 and TRAF4 is highly expressed in GBM tissues and promotes proliferation of GBM cells. (spandidos-publications.com)
Glioma tumor tissues were collected from 3 patients who received surgery, and the glioma stem cells were then separated, cultured, and identified by flow cytometry. (medscimonit.com)

Thus, suppression of cell proliferation in the hippocampus could constitute one of the mechanisms that underlie depression, and physical activity might be an efficient antidepressant. (biopsychiatry.com)
This study showed that YAP upregulation promoted CNV formation by upregulating the proliferation of endothelial cells, providing evidence for the molecular mechanisms of CNV and suggesting a novel molecular target for nAMD treatment. (molvis.org)
Overall, the results suggest that ES cells use different mechanisms as mature cells to coordinate their proliferation rate with ribosome biogenesis. (uni-muenchen.de)
The underlying molecular mechanisms by which androgen-independent PC cells acquire the capacity to proliferate remain largely unclear. (go.jp)
Other cell-mediated mechanisms (especially inflammation). (cdc.gov)
Other cell-mediated mechanisms (especially inflammation induced by TNF-α). (cdc.gov)
Here we explore potential mechanisms how the proliferation and contractility of gubernaculum testis cells are regulated by diethylstilbestrol . (bvsalud.org)

We isolated stromal cells from endometrial cancer patients to examine local biosynthesis of estrogens and tumor-stromal interactions. (scirp.org)
Since hypoxia activates hypoxia-inducible factor (HIF-1)-dependent transcription in cancer cells that, in a paracrine fashion, drive tumor angiogenesis, we investigated the effects of UCN-01 on HIF-1-responsive promoter constructs. (karger.com)
Treatment of mouse NIH3T3 cells with 5FU, a potent inhibitor of rRNA maturation, confirmed an activation of this checkpoint, leading to weak induction of the tumor suppressor p53, induction of the Cdk-inhibitor p21, an increase in active, hypo-phosphorylated Rb, and to accumulation of cells in the G1- and S-phase with an increase of cells in G1-phase. (uni-muenchen.de)
The present study revealed that E2F1 gene expression correlates with TS and Survivin gene expressions and tumor proliferation. (aacrjournals.org)
In conditions with primary optic atrophy (eg, pituitary tumor, optic nerve tumor, traumatic optic neuropathy, multiple sclerosis), optic nerve fibers degenerate in an orderly manner and are replaced by columns of glial cells without alteration in the architecture of the optic nerve head. (medscape.com)

In colon-derived cell lines where Yap is overabundant, its depletion strongly reduces beta-catenin and Notch signaling and inhibits proliferation and survival. (harvard.edu)
Accordingly, we aimed to evaluate the effects of metformin in the survival and proliferation of PHEO cells. (endocrine-abstracts.org)

We report that, in addition to direct inhibitory effects on endothelial cell growth, UCN-01 abrogates hypoxia-mediated transactivation of HIF-1-responsive promoters in a prostate cancer cell line. (karger.com)
The mechanism of OGF-OGFr action on DNA synthesis was related to the cyclin-dependent kinase inhibitory pathway because knockdown of p16 or p21 in OVCAR-3 cells, and p21 in SKOV-3 cells, eliminated OGF's inhibitory effect on growth. (nih.gov)

Studies have shown that various signaling pathways serve crucial roles in the tumorigenesis of UCEC, amongst which the Wnt/β-catenin pathway is of great interest due to its crucial role in cell proliferation and the huge potential as a therapeutic target. (medsci.org)
This study investigated the effect of EZH2 expression on proliferation and tumorigenesis of brain glioma cells. (medscimonit.com)
Our data demonstrated that in brain glioma cells, the decrease of EZH2 level could suppress cell proliferation and tumorigenesis potency, and meanwhile inhibit the expressions of oncogenes including c-myc and Akt. (medscimonit.com)

They arise from cancerous brain and spinal cord glial cells ( 1 , 2 ). (spandidos-publications.com)
Optic nerve fibers exhibit marked degeneration, with excessive proliferation of glial tissue. (medscape.com)

SOX4 has been revealed to induce EMT, a key process during organ development and the progression of epithelial tumors to metastatic cancers ( 9 ), via its activation of the Wnt and transforming growth factor β signaling pathways in cancer cells ( 10 , 11 ). (spandidos-publications.com)
Metformin has been shown to have antiproliferative properties in several cancer cell lines, possibly related to its ability to inhibit cell proliferation pathways. (endocrine-abstracts.org)

Fig. 8: Selective deletion of Adra1a from OPCs reduces their proliferation. (nature.com)

Human bronchial epithelial cells (Beas2B) were continuously exposed to nFe2O3 or nano-SiO2 coated nFe2O3 (SiO2-nFe2O3) for up to 6.5 months at an occupationally relevant low dose (0.6 microg/cm2 or 2.88 microg/mL) and evaluated over time for indications of neoplastic-like transformation and its underlying mechanism. (cdc.gov)

A common trait of antidepressant drugs, electroconvulsive treatment and physical exercise is that they relieve depression and up-regulate neurotrophic factors as well as cell proliferation and neurogenesis in the hippocampus. (biopsychiatry.com)
In order to identify possible biological underpinnings of depression and the antidepressant effect of running, we analysed cell proliferation, the level of the neurotrophic factor BDNF in hippocampus and dynorphin in striatum/accumbens in 'depressed' Flinders Sensitive Line rats (FSL) and Flinders Resistant Line (FRL) rats with and without access to running-wheels. (biopsychiatry.com)
In the hippocampus, cell proliferation was lower in the 'depressed' rats compared to the control FRL rats but there was no difference in BDNF or dynorphin levels in striatum/accumbens. (biopsychiatry.com)
The only parameter that was associated to basal level of 'depression' and to the antidepressant effect was cell proliferation in the hippocampus. (biopsychiatry.com)
Ki67-labelled cells were counted within the dentate gyrus and hilus on sections taken from the anterior hippocampus. (brainresearchlab.com)

These findings indicate that sesamin inhibits cervical cancer cell proliferation, and its mechanism may be attributed to the induction of p53/PTEN-mediated apoptosis. (medsci.org)
Our findings provide the first evidence that PLC-Ca2+ -CREB signalling pathway mediates the nongenomic effects of diethylstilbestrol on gubernaculum testis cells . (bvsalud.org)

Using OVCAR-3 and SKOV-3 ovarian cancer cell lines, we found that OGF and OGFr were present and functional. (nih.gov)
The repressive effect of OGF on cell proliferation also was observed in SW626, CAOV-3, and HEY ovarian cancer cell lines. (nih.gov)
These data are the first to report that the OGF-OGFr system is a native biological regulator of cell proliferation in human ovarian cancer. (nih.gov)
Ovarian granulosa cells from a rat model of PCOS were assigned to control and si-Prok1 groups, after cell culture. (ajol.info)

Treatment with honey stops the tumour cells cycle by regulating some genes (cyclin D1, CDK2, p27Kip. (agenciasinc.es)
K. Kokame, H. Kato and T. Miyata, "Homocysteine-Respondent Genes in Vascular Endothelial Cells Identified by Differential Display Analysis. (scirp.org)
We investigated the clinical significance of E2F1 gene expression in relation to its target genes, thymidylate synthase ( TS ) and Survivin , in case of non-small-cell lung cancer (NSCLC). (aacrjournals.org)
DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)

Next we examined the effects of steroid sulfatase inhibitor and aromatase inhibitor in monoculture of endometrial cancer cell line (Ishikawa) and in a co-culture system involving an Ishikawa cells and stromal cells. (scirp.org)
We evaluated the effects of STS inhibitor and aromatase inhibitors on the proliferation of estrogen-dependent endometrial cancer cells. (scirp.org)
MiR-506 mimics, inhibitor, and negative control (NC) were transfected into MDA-MB-231 breast cancer cells. (medscimonit.com)
In this study, mouse gubernaculum testis cells were pretreated with phospholipase C (PLC) inhibitor U-73122 and then treated with DES. (bvsalud.org)

We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)
95% of the global population, and can give rise to a number of malignancies in B cells and epithelial cells. (duke.edu)
It is likely that miR-BARTs play a crucial role in EBV-infected epithelial cells, however a comprehensive understanding of miR-BARTs is currently lacking. (duke.edu)

Diethylstilbestrol (DES) is a nonsteroidal synthetic estrogen that disrupts the morphology and proliferation of gubernaculum testis cells , but the underlying mechanism is unclear. (bvsalud.org)

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (6). (cellsignal.com)
Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 7). (cellsignal.com)
After mitosis, the cell will undergo the G 0 /G 1 phase when the cells rest to become ready for the next round of replication. (biolegend.com)
For additional reagents (including antibodies) on cell cycle analysis or DNA replication, check here . (biolegend.com)
Silencing of OGFr using siRNA technology stimulated cell replication, documenting its integral role. (nih.gov)
Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)

Unpredictably, cell cycle progression, cell proliferation and DNA synthesis were unaffected fol lowing NDRG1 expression. (scirp.org)
However, the pathway downstream NDRG1, involving p21 and c8/18, regulating cell cycle progression and DNA synthesis is unfunctional. (scirp.org)
Generally, cells have to closely coordinate growth and cell cycle progression during proliferation to prevent premature division. (uni-muenchen.de)

We demonstrated that bradykinin (BK), through the B2 receptor, induced a dose-dependent inhibition of mesangial cell proliferation stimulated by fetal calf serum. (nih.gov)
Taken together, these data suggest for the first time that, in proliferating rat mesangial cells, B2 receptor stimulation is able to induce, via a pertussis insensitive pathway, the inhibition of tyrosine kinase activity and mesangial cell proliferation. (nih.gov)
It is produced by Idiopathic nephrotic syndrome (INS) a variety of cells, including monocytes and accounts for 90% of nephrosis in child- mesangial cells in the kidney [ 9 ]. (who.int)

Inhibition of p53 by pifithrin-α significantly reduced the levels of PUMA, Bax, and PTEN but restored AKT phosphorylation in SiHa cells exposed to sesamin. (medsci.org)

Sample suitability indicates whether this reagent can be used to label already fixed tissue sections or single cells in suspension or cell culture. (biolegend.com)
There can be symmetrical or asymmetrical division depending on the cell and tissue type. (biolegend.com)
A fat cell is an animal connective tissue cell specialized for the synthesis and storage of fat. (mcw.edu)
Method: We measured the cell proliferation marker, Ki67, in post-mortem hippocampal tissue from patients with schizophrenia ( n = 10) and matched controls ( n = 16). (brainresearchlab.com)
The presence of asbestos fibers in the lungs sets off a variety of responses leading to inflammation, cell, and tissue damage, which can lead to malignant and non-malignant diseases. (cdc.gov)

To observe the proliferation and adhesion of umbilical cord endothelial cells (HUVEC) on the titanium (Ti) surface with large particles blasted-etched (SLA) treatments and further clarify the mechanism of action of each other between the Ti implant surface and the body. (atlantis-press.com)
We investigated the AhR-dependent mechanism of action of flutamide in human hepatocellular carcinoma cells and identified that transforming growth factor-β1 (TGF-β1) is induced by flutamide in an AhR-dependent manner. (oregonstate.edu)
A potential mechanism for the role of calcium in prostate cancer development and progression is that intracellular calcium controls the growth of prostate cancer cells and the process of apoptosis (9). (cdc.gov)

However, its proliferation was significantly decreased by the treatment of steroid sulfatase or aromatase inhibitors. (scirp.org)

Cell Biochem Funct;36(1): 13-17, 2018 Jan. (bvsalud.org)

Proliferation of the cancer cells was significantly increased through the steroid sulfatase pathway, which metabolizes androgens, estrone sulfate, and estradiol sulfate as its substrates. (scirp.org)
Calcein-AM is used for the kit because it is retained inside of cells better than the other fluorescent esterase substrates. (dojindo.com)

The results, published in the Journal of Functional Foods, show that strawberry tree honey is capable of inhibiting the proliferation of tumour cells cultivated in laboratory plates - more specifically, those of a line of human colon adenocarcinoma (HCT-116) and another with metastatic characteristics (LoVo), widely used to investigate this type of tumour. (agenciasinc.es)
This led to other relevant results, such as the reduction of some transcription factors, the inhibition of the activity of certain enzymes and the decrease of mitochondrial respiration and glycolysis of tumour cells. (agenciasinc.es)
Results: We replicated the finding of a significant reduction in Ki67+ cells/mm 2 in schizophrenia cases compared to controls ( t 24 = 2.1, p = 0.023). (brainresearchlab.com)
Our results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. (aacrjournals.org)
Given the high frequency of DDX5 amplification in breast cancer, our results highlight DDX5 as a promising candidate for targeted therapy of breast tumors with DDX5 amplification, and indeed we show that DDX5 inhibition sensitizes a subset of breast cancer cells to trastuzumab. (aacrjournals.org)
The results demonstrated that U-73122 impaired DES-evoked intracellular Ca2+ mobilization in gubernaculum testis cells and inhibited DES-induced proliferation of gubernaculum testis cells . (bvsalud.org)

During the progression of NSCLC, E2F1 overexpression could produce more aggressive tumors with a high proliferation rate and chemoresistance. (aacrjournals.org)

Exposure to radiation can damage the basal cell layer of the skin and result in inflammation, erythema, and dry or moist desquamation. (cdc.gov)

Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. (mdpi.com)
Ablation of the kinases Mst1 and Mst2, orthologs of the Drosophila antiproliferative kinase Hippo, from mouse intestinal epithelium caused marked expansion of an undifferentiated stem cell compartment and loss of secretory cells throughout the small and large intestine. (harvard.edu)
Thus, supraphysiologic Yap polypeptide levels are necessary to drive intestinal stem cell proliferation. (harvard.edu)

We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
Strawberry tree honey is capable of inhibiting the proliferation of tumour cells on a line of human colon adenocarcinoma (HCT-116) and another with metastatic characteristics (LoVo). (agenciasinc.es)
Here, we investigate the effects of the photovoltaic activity of p-i-n nanowire arrays on A549 human lung adenocarcinoma cells. (lu.se)
Human HeLa and SiHa cervical cancer cells and normal Hs68 dermal cells were used as cell models. (medsci.org)
Furthermore, human cDNA of NDRG1 from normal placenta was cloned into a eukaryotic expression vector and transfected into the three cancer cell lines. (scirp.org)
Yap is overexpressed in 68 of 71 human colon cancers and in at least 30 of 36 colon cancer-derived cell lines. (harvard.edu)
To evaluate the proliferative capacity of mesenchymal cells derived from human periodontal ligament on polished and plasma-treated titanium surfaces. (bvsalud.org)
We concluded that plasma nitriding produced surfaces that permitted the proliferation of human periodontal ligament mesenchymal cells. (bvsalud.org)
Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. (wikipedia.org)

Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)

GSH accumulation in the nucleus is triggered in Arabidopsis cells in culture by treatments that synchronize cells at G1/S as identified by flow cytometry and marker transcripts. (cam.ac.uk)

Sesamin (75 and 150 μM) clearly inhibited SiHa and HeLa cell proliferation in a dose-dependent fashion, but did not affect the proliferation of Hs68 cells. (medsci.org)

In this context, complement receptor 4 (CR4), typically a dendritic cell marker, has also been shown to be sharply increased with A plaque load throughout all stages of AD. (nos-nop.org)

Immunohistochemical analysis of paraffin embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Phospho-Histone H3 (Ser10) Antibody, PCNA (PC10) Mouse mAb, Cleaved Caspase-3 (Asp175) Antibody and Survivin (71G4B7E) Rabbit mAb. (cellsignal.com)

The critical challenge in cancer treatment is how to effectively kill cancer cells without affecting healthy cells. (hindawi.com)
Estrogen receptor and steroid sulfatase mRNA levels in cancer cells were significantly higher in the co-cultures compared with the monocultures of endometrial cancer cells. (scirp.org)
Spanish and Italian researchers have proven that when honey from strawberry trees, a product typical of Mediterranean areas, is added to colon cancer cells grown in the laboratory, cell proliferation is stopped. (agenciasinc.es)
Here, we explored whether sesamin activates p53, which is widely inhibited in cervical cancer cells, thereby inducing p53-mediated apoptosis. (medsci.org)
miR-506 over-expression may thus be able to improve the malignant phenotype of breast cancer cells. (medscimonit.com)

Proliferating cell nuclear antigen (PCNA) expression in CNV mice without or with YAP siRNA intravitreal injection and the colocalization of PCNA and CD31 were measured with western blotting and immunofluorescent double staining, respectively. (molvis.org)
Additionally, after laser exposure, YAP siRNA inhibited the increased expression of PCNA, which was colocalized with endothelial cells. (molvis.org)
Then, control lentivirus and Prok1 siRNA lentivirus (50 μL each) were added to the cells to the groups, respectively. (ajol.info)

The proliferation of choroidal endothelial cells (CECs) is one important step in the formation of new vessels. (molvis.org)
The bone-marrow-derived endothelial progenitor cells (EPCs) provide an alternative source of endothelial cells (ECs) that contributes to neovessel formation in endothelium structure [ 4 , 5 ]. (hindawi.com)
Localization of the proteins was predominantly nucleolar and the formation of a stable complex (PeBoW-complex), including all three proteins, was experimentally validated in mature mouse cells as well as in mouse ES cells. (uni-muenchen.de)

Anatomy42

Organisms13

Diseases13

Chemicals and Drugs100

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes56

Disciplines and Occupations1

Information Science3

Differentiation22

Signaling pathway5

Inhibit proliferation1

Colony formation3

Endothelial cell proliferation1

Inhibition of cell proliferation1

Hippocampal cell proliferation2

Vitro8

Control of cell proliferation3

Assay16

Migration4

Apoptosis and prom1

Protein14

Metastasis1

Promote the proliferation1

Division and proliferation2

Suppresses4

Cellular10

Methods4

Assays7

Apoptotic4

Viability4

Tissues2

Mechanisms7

Tumor5

Survival2

Inhibitory2

Tumorigenesis3

Glial2

Pathways2

Reduces1

Neoplastic1

Hippocampus5

Findings2

Ovarian4

Genes4

Inhibitor4

Epithelial3

Morphology1

Replication6

Cycle progression3

Mesangial3

Phosphorylation1

Tissue5

Mechanism3

Significantly decreased1

20181

Substrates2

Results6

Progression1

Inflammation1

Intestinal3

Human9

Regulator1

Flow Cytometry1

Dose-dependent1

Marker1

Antibody1

Cancer cells5

SiRNA3

Formation3