Normally cell microdissection. Medical search. Frequent questions

Fluidigm has launched the AccuLift Laser Capture Microdissection System, which enables precise and efficient capture of individual cells or larger tissue regions from frozen and FFPE tissue sections for DNA, RNA, and protein biomarker analysis using sequencing, real-time PCR, or mass spectrometry. (genomeweb.com)

NEW YORK - Researchers at the University of California, Santa Cruz have pioneered a method for repeatedly sampling messenger RNA (mRNA) from single cells without killing the cells in question. (genomeweb.com)
In the proof-of-concept study, Pourmand's group aspirated 22 cells within a breast cancer tissue slice and selectively captured the mRNA using Nvigen's proprietary antibody-coated magnetic nanoparticles. (genomeweb.com)
Hundreds of genes were sequenced from each cell at each aspiration, and the genes sequenced showed high correlations from one sampling to the next, indicating that the small amount of mRNA withdrawn from each cell did significantly affect gene coverage. (genomeweb.com)
Where most scRNA-seq methods involve killing the cell from which messenger RNA is taken, the UCSC method leaves cells alive for further sampling, taking only between 1 and 5 percent of the cell's mRNA per sampling event. (genomeweb.com)
Upon activation, a light-activated biotin tag is used to bind the poly-A tails of mRNA, capturing mRNA within live cells and intact tissues in a relatively noninvasive manner. (genomeweb.com)
Targeted disruption of the gene encoding D4Rs reduces the amplitude of the contrast sensitivity rhythm by reducing daytime sensitivity and abolishes the rhythmic expression of Npas2 and Adcy1 mRNA in the ganglion cell layer (GCL) of the retina. (jneurosci.org)
Neurone, astrocyte, and endothelial cell-enriched mRNA, obtained by immuno-laser capture microdissection of temporal cortex (Brodmann area 21/22) from 6 cases with self-reported T2D in the Cognitive Function and Ageing Study neuropathology cohort, and an equal number of age and sex-matched controls, was assessed by microarray analysis. (biomedcentral.com)

Pan LXDiss TCPeng HZIsaacson PG Clonality analysis of defined B-cell populations in archival tissue section using microdissection and the polymerase chain reaction. (jamanetwork.com)
Analysis of immunophenotypically defined cell populations markedly increased specificity of liquid chromatography-tandem mass spectroscopy (LC-MS/MS). Over 375 differentially expressed proteins were analyzed by Ingenuity Pathway Analysis (Ingenuity Systems). (genengnews.com)
Peking) roots infected by incompatible (I) and compatible (C) populations of soybean cyst nematode [SCN] (Heterodera glycines) were collected using laser capture microdissection. (usda.gov)
Highly enriched cell populations were obtained and subjected to gene expression analysis. (ku.dk)
And next-generation sequencing (NGS) libraries can be used to quantify spatial gene expression patterns that delineate between cell populations. (scienceboard.net)
However, the vast majority of biological knowledge has emerged as a consequence of studying cell populations and not individual cells. (justia.com)
Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
The kit delivers exceptional genome coverage from single cells, populations of cells and low quantities of genomic DNA, including regions with high GC-content. (qiagen.com)

Expression of DCT was absent or barely detected in retina tissues, various human ocular cells, and major organs. (molvis.org)
This involves looking under the microscope and guiding a laser to cut away specific cells from areas of the brain and placental tissues, so scientists could measure the amount of Zika virus genetic materials (RNA) in specific cells. (cdc.gov)
The high diagnostic potential of peptides and proteins from cells, tissues, and biological fluids is unquestionable. (genengnews.com)
Our team was the first to conduct an in-depth molecular comparison of cancer and normal tissues for the presence of cancer stem cells," says David M. Lubman, Ph.D., professor of surgery at the University of Michigan, Ann Arbor. (genengnews.com)
The wide range of manifestations observed in patients with mitochondrial disease results from varying fractions of abnormal mtDNA molecules in different cells and tissues, a phenomenon termed heteroplasmy. (jci.org)
However, the landscape of heteroplasmy across cell types within tissues and its influence on phenotype expression in affected patients remains largely unexplored. (jci.org)
Spatially resolved molecular atlases help scientists understand where different types of cells are located in the body and map their gene expression in specific locations in tissues and organs. (scienceboard.net)
Alternatively, gene expression patterns can be resolved using laser capture microdissection (LCM), which samples tissues on a grid. (scienceboard.net)
Enthesis development has historically been studied with lineage tracing of individual genes selected a priori, which does not allow for the determination of single-cell landscapes yielding mature cell types and tissues. (elifesciences.org)
The MMI CellCut supports precise and contamination-free analysis of cell groups, single cells or subcellular parts from various types of tissues including fresh frozen, paraffin-embedded and archived slides, cytospins, smears and even living cells. (dssimage.com)
Stomach tissues were harvested, followed by histologic and immunofluorescence staining, organoid culture, flow cytometry to isolate gastric corpus isthmus and nonisthmus epithelial cells, and RNA extraction for transcriptomic analysis. (bvsalud.org)

As part of his Ph.D. he analyzed the gene expression of human dopaminergic neurons, which he isolated from post mortem midbrain tissue of Morbus Parkinson patients and control subjects using a laser microdissection system from Leica Microsystems. (leica-microsystems.com)
From Eye to Insight is therefore fitting perfectly to Leica Microsystems and in particular to laser microdissection. (leica-microsystems.com)

DNA mutations lead to abnormal proteins or missing functional proteins, which can cause cells to multiply uncontrollably and become cancerous. (leica-microsystems.com)
Germinal-center B-cells undergo clonal expansion and activate the process of somatic hypermutation that introduces mutations at a high rate into rearranged immunoglobulin variable genes. (medscape.com)
Germinal-center B-cells with unfavorable mutations are functionally crippled and undergo programmed cell death (apoptosis). (medscape.com)
Low background and comprehensive coverage make this kit highly suited for the analysis of aneuploidy, copy number variations and mutations from single cells and low-input samples. (qiagen.com)

Recently however, there is a new hypothesis in which fetal microchimeric cells could play a role in the development of an auto-immune disease. (ugent.be)
During pregnancy, there is a transfer of fetal cells, through the placenta, from the fetus to the blood circulation of women. (ugent.be)
Fetal cell microchimerism is presumed to be associated with some maternal autoimmune diseases such as systemic sclerosis and autoimmune thyroid diseases because of the high incidence in women post-partum and because of the similarities with graft-versus-host disease. (ugent.be)
Detection and further characterization of the male fetal cells in women post-partum with an autoimmune disease may result in a better understanding of the pathogenesis of the disease. (ugent.be)
Male fetal cells can be distinguished from female maternal cells by fluorescence in situ hybridization (FISH) with CEP X SpectrumOrange/ CEP Y SpectrumGreen DNA probes. (ugent.be)
The detected fetal cells will be further characterized using monoclonal antibodies to determine their possible role in auto-immune diseases. (ugent.be)
We speculate that disturbed development of somatic cells in the fetal testis may play a role in allowing undifferentiated cells to survive in the postnatal testes. (ku.dk)
The best evidence suggests that most are due to abnormal differentiation of fetal germ cells that arise from the fetal yolk sac. (medscape.com)

Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
Using the strongest molecular binding partnership in biology to separate different cell types. (the-scientist.com)
1997-2001: Chercheur associé au Department Neuroscience and Cell Biology, Rutgers Univ/UMDNJ, Etats-Unis. (univ-nantes.fr)
We identified several genes that were highly expressed in SCN-resistant compared to susceptible soybean root cells using sophisticated molecular biology techniques. (usda.gov)
QIAseq Single Cell DNA Library Kits UDI are intended for molecular biology applications. (qiagen.com)

The LCM enables a wide range of applications ranging from single-cell isolation to large biopsy extractions. (concordia.ca)
As Pourmand suggests in his group's published study, theirs does appear to be the only technique currently available that enables repeat sampling of the same cell at scale. (genomeweb.com)

Such development pattern shares an overlapping biological behavior with the growth plate, which is a process of mesenchymal stem cells differentiating into chondrogenic cells and then sequentially into fibrocartilage cells ( Killian, 2022 ). (elifesciences.org)

Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
Clonal rearrangements of T-cell receptor genes in mycosis fungoides and dermatopathic lymphadenopathy. (jamanetwork.com)
Langerak AWScczepanski Tvan der Burg MWolvers-Tettero ILvan Dongen JJ Heteroduplex PCR analysis of rearranged T-cell receptor genes for clonality assessment in suspect T-cell proliferations. (jamanetwork.com)
To study epigenetic changes and genes that are expressed differently in different cell lineages-such as cancer cells versus normal cells, or the two types of epidermal cells in Arabidopsi s roots-typically requires laser capture microdissection (LCM) or fluorescence-activated cell sorting (FACS). (the-scientist.com)
We compared gene expression patterns in soybean root cells being fed upon by SCN in both SCN-resistant and susceptible soybean to determine if we could identify genes that are highly expressed in resistant root cells and not highly expressed in susceptible cells. (usda.gov)
These genes may provide clues as to how soybean cells can be resistant to SCN. (usda.gov)
Our analyses identified differentially expressed genes in syncytial cells microdissected from roots undergoing I and C reactions. (usda.gov)
In syncytial cells from I roots at three days post infection (dpi), genes encoding lipoxygenase, heat shock protein 70, and superoxidase dismutase were elevated almost 10-fold or more, while genes encoding several transcription factors and DNA binding proteins were also elevated, albeit at lower levels. (usda.gov)
In syncytial cells formed during the C interaction at three dpi, genes encoding prohibitin, the epsilon chain of ATP synthase, allene oxide cyclase and annexin were highly expressed. (usda.gov)
Unique expression patterns for these developmentally very related cell types revealed that CIS cells were very similar to gonocytes because only five genes distinguished these two cell types. (ku.dk)
For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
With this technology, we are getting closer to the goal of a widely clinically applicable liquid biopsy, where we can sample cancer cells by a simple blood draw and understand the genes that allow them to grow," said Dr. Antoni Ribas, a professor of medicine in the division of hematology-oncology, a Jonsson Cancer Center member and one of Tseng's key collaborators. (sciencedaily.com)
912, 2202, and 1227 genes were significantly differentially expressed between cases with self-reported T2D and controls in neurones, astrocytes, and endothelial cells respectively. (biomedcentral.com)
Mutter suggested that these genes or the cells have only the maternal imprint because many teratomas arise from a parthenogenetically activated egg. (medscape.com)

Another method called Geo-seq combines scRNA-seq with laser capture microdissection. (genomeweb.com)
we applied Single-cell RNA sequencing (scRNA-seq) to delineate the comprehensive postnatal RC enthesis growth and the temporal atlas from as early as postnatal day 1 up to postnatal week 8. (elifesciences.org)

Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)
Fluorescence and isotope tagging are the principal means for measuring antibody binding to cells in flow cytometry. (nature.com)
You will learn retinal anatomy and the techniques of ocular microdissection, cryosectioning, plastic sectioning and fluorescence microscopy. (upenn.edu)
CellCelector Robotic System service: The ALS CellCelector is a freely configurable tool for the automated picking of single cells, cell colonies and cell clusters utilizing morphological and fluorescence features of a target. (weizmann.ac.il)

Identification of crucial cancer biomarkers requires analysis of a pure and homogeneous cell population. (genengnews.com)

Both of these latter techniques risk changes to the metabolism during cell preparation and barcoding prior to cell lysis- since both approaches, generally, rely on the use of live cells 30 . (nature.com)
The kit includes all reagents required for cell lysis, whole genome amplification using REPLI-g DNA polymerase, enzymatic DNA fragmentation and PCR-free NGS library preparation compatible with Illumina NovaSeq and other NGS instruments. (qiagen.com)
After cell lysis, a high-fidelity MDA reaction is used to amplify microgram amounts of gDNA using the high-fidelity REPLI-g DNA Polymerase. (qiagen.com)
The kit contains reagents for the effective cell lysis of isolated single cells or small clusters of cells, for example from laser-capture microdissection platforms. (qiagen.com)

We isolated four categories of cells from mammary epithelium of female calves: bromodeoxyuridine label retaining epithelial cells (LREC) from basal (LRECb) and embedded layers (LRECe), and epithelial control cells from basal and embedded layers. (frontiersin.org)
This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
Transcriptomic analyses of the isolated epithelial cells showed that the cell-cycle-related MYC target gene signature was enriched in the corpus epithelial cells of RUNX3R122C/R122C mice compared with the wild-type corpus. (bvsalud.org)
K17 was not detected in the epithelial cells of benign glandular lesions, but groups of cuboidal cells, residing beneath the epithelial layer of benign glands, were frequently positive for K17, especially in cases of microglandular hyperplasia. (medscape.com)

These approaches result in the loss of all histological information pertaining to the in vivo locale of MaSC and progenitor cells. (frontiersin.org)
Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)
MaSC/progenitor cells were identified based on their ability to retain bromodeoxyuridine for an extended period. (frontiersin.org)
as well as a dramatic increase in the number of rapidly proliferating isthmus stem/progenitor cells in the corpus of RUNX3R122C/R122C mice. (bvsalud.org)
CONCLUSIONS: RUNX3R122C missense mutation is associated with the continuous cycling of isthmus stem/progenitor cells, maturation arrest, and development of a precancerous state. (bvsalud.org)

Testicular germ cell cancers in young adult men derive from a precursor lesion called carcinoma in situ (CIS) of the testis. (ku.dk)

GAMDDL analysis represents a novel and useful molecular method for examining the precise cell lineages of primary and secondary tumours, and was more accurate than HUMARA in determining clonality. (nih.gov)

Prognostic significance of a polymerase chain reaction-detectable dominant T-cell clone in cutaneous lesions of patients with mycosis fungoides. (jamanetwork.com)
Genotypic analysis of cutaneous T-cell lymphoma: a comparative study of southern blot analysis with polymerase chain reaction amplification of the T-cell receptor-gamma gene. (jamanetwork.com)
Diss TCWatts MPan LXBurke MLinch DIsaacson PG The polymerase chain reaction in the demonstration of monoclonality in T-cell lymphomas. (jamanetwork.com)
Detection of clonal T-cell receptor gamma gene rearrangements in early mycosis fungoides/Sézary syndrome by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE). (jamanetwork.com)
Scheller UMuche JMSterry WLukowsky A Detection of clonal T cells in cutaneous T-cell lymphoma by polymerase chain reaction: comparison of mutation detection enhancement-polyacrylamide gel electrophoresis, temperature gradient gel electrophoresis and fragment analysis of sequencing gels. (jamanetwork.com)
Tok JSzaboles MJSilvers DNZhong JMatsushima AY Detection of a clonal T-cell receptor gamma chain gene rearrangements by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE) in archival specimens from patients with early cutaneous T-cell lymphoma: correlation of histologic findings with PCR/DGGE. (jamanetwork.com)
Utility of microdissection and polymerase chain reaction for the detection of immunoglobulin gene rearrangement and translocation in primary intraocular lymphoma. (jamanetwork.com)

HL-RSC carry clonal immunoglobulin gene rearrangements, which establishes their clonality and their B cell origin. (medscape.com)

Global expression profiling of cells of the organ of Corti and spiral ganglion following noise exposure will be used to identify pathways induced or influenced by NIHL. (uhhospitals.org)
Dr. Sieck has developed an extensive array of state-of-the-art physiological, molecular and biomedical engineering techniques to explore cell signaling pathways. (mayo.edu)
Alterations in gene sequence or expression can occur in the cell-signalling and regulatory pathways involved in cell-cycle control, apoptosis, proteosome regulation and angiogenesis. (bmj.com)
Changes in cortical neurones included alterations in insulin and other signalling pathways, cell cycle, cellular senescence, inflammatory mediators, and components of the mitochondrial respiratory electron transport chain. (biomedcentral.com)

Normal migration of these germ cells may cause gonadal tumors, whereas abnormal migration produces extragonadal tumors. (medscape.com)

The main topic during this course was how to apply laser microdissection in neuroscience. (leica-microsystems.com)

Our analysis, for example the RNA quality of the specimens, showed that the quality of the results is not influenced by the staining process and laser microdissection. (leica-microsystems.com)
Bottaro MBerti EBiondi AMigone NCrosti L Heteroduplex analysis of T-cell receptor gamma gene rearrangements for diagnosis and monitoring of cutaneous T-cell lymphomas. (jamanetwork.com)
Chhanabhai MAdomat SAGascoyne RDHorsman DE Clinical utility of heteroduplex analysis of TCR gamma gene rearrangements in the diagnosis of T-cell lymphoproliferative disorders. (jamanetwork.com)
Transcriptome in vivo analysis (TIVA), for example, enters cell membranes via a cell-penetrating peptide. (genomeweb.com)
We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. (nature.com)
This paper represents a valuable single-cell level analysis of tendon enthesis development. (elifesciences.org)
Moreover, the device does not allow isolated cells to be washed from the supernatant prior to treatment or analysis. (justia.com)
Accordingly, a goal of microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. (justia.com)
Microfluidic systems provide numerous advantages for single cell analysis: economies of scale, parallelization and automation, and increased sensitivity and precision that comes from small volume reactions. (justia.com)
Considerable effort over the last decade has been directed towards developing integrated and scalable single cell genetic analysis on chip (7, 8). (justia.com)
Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
Although these systems provide a high-throughput qPCR readout, they do not address the front end sample preparation and require single cell isolation by FACS or micropipette followed by off-chip processing and pre-amplification of starting template prior to analysis. (justia.com)
The critical step of integrating all steps of single cell analysis into a robust system capable of performing measurements on large numbers of cells has yet to be reported. (justia.com)
With the improvements to their device, which uses a Velcro-like nanoscale technology, they can now detect and isolate single cancer cells from patient blood samples for analysis. (sciencedaily.com)
When blood is passed through the chip, extremely small "hairs" -- nanoscale wires or fibers coated with protein antibodies that match proteins on the surface of cancer cells -- act like Velcro, traping CTCs and isolating them for analysis. (sciencedaily.com)
The preservation of single captured CMCs in this proof-of-concept study also allowed researchers to conduct an analysis -- called single-cell genotyping -- to find within the cell a specific target (BRAF V600E) for a drug called vemurafenib. (sciencedaily.com)
Due to the PCR-free workflow, this kit is highly suited for the analysis of aneuploidy, copy number and sequence variation in single cells from rare samples. (qiagen.com)

Different kinds of techniques involve microdissection: Chromosome microdissection - use of fine glass needle under a microscope to remove a portion from a complete chromosome. (wikipedia.org)

3) Use FACS to isolate single retinal cell types and determine the effects of iron transporter knockouts on their transcriptome. (upenn.edu)

The further development of CIS into invasive germ cell tumors may depend on signals from their postpubertal niche of somatic cells, including hormones and growth factors from Leydig and Sertoli cells. (ku.dk)
Teratomas (from Greek teras, meaning "monster," and - oma, a suffix denoting a tumor or neoplasm) and other germ cell tumors are relatively common solid neoplasms in children. (medscape.com)

Previous work in our laboratory identified keratin 17 (K17) as a specific and sensitive biomarker for high-grade squamous intraepithelial lesions and cervical squamous cell carcinoma (SCC). (medscape.com)
Cervical cancer has multiple histologic subtypes, with squamous cell carcinoma (SCC) accounting for approximately 75% of cases and invasive adenocarcinoma accounting for 10% to 15% of cases. (medscape.com)
[ 11-13 ] By contrast, we discovered, using laser capture microdissection and mass spectrometry, that keratin 17 (K17) could predict the overall survival of patients with cervical squamous cell carcinoma more accurately than grade, stage, or any other clinicopathologic features. (medscape.com)

Falk Schlaudraff (Institute of General Physiology of the University of Ulm, Germany) used modern laser microdissection methods to isolate and analyze cells from post mortem tissue specimens taken from M. Parkinson patients in order to gain molecular insight into the disease. (leica-microsystems.com)
The ability to capture and preserve single CMCs allows doctors to analyze melanoma cells' DNA structure, determine the genetic characteristics of the patient's cancer and confirm that the circulating cells remain genetically similar to the tumor they came from. (sciencedaily.com)

The expression profile of CIS cells was compared with microdissected gonocytes, oogonia, and cultured embryonic stem cells with and without genomic aberrations. (ku.dk)
The QIAseq Single Cell DNA Library Kit UDI provides a complete solution for whole genome sequencing from isolated single cells or low amounts of genomic DNA. (qiagen.com)

While this technique allows scientists to explore single-cell transcriptomes in the context of their natural microenvironment and with clear spatial resolution, it is relatively low throughput with regard to the number of cells that can be analyzed. (genomeweb.com)

Quantum barcoding (QBC) avoids isolation of single cells by building cell-specific oligo barcodes dynamically within each cell. (nature.com)

Dr. Sieck's research team has shown that at the level of phrenic motor neurons, functional recovery is enhanced by intrathecal BDNF treatment as well as the use of locally implanted mesenchymal stem cells that are genetically engineered to produce BDNF . (mayo.edu)

The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)
We developed a qPCR-based platform that could be used to isolate individual cells from native tissue and obtain highly comparable gene expression analyses. (leica-microsystems.com)
These observations demonstrate that gene expression is different in syncytial cells as compared to whole roots infected with nematodes. (usda.gov)
Our observations also show that gene expression is different between syncytial cells that were isolated from I and C roots and that gene expression is changing over the course of syncytial cell development as it matures into a functional feeding site. (usda.gov)
Gene expression quantification of the sections is implemented using spatial transcriptomics by reoriented projections and sequencing (STRP-seq), a method that combines the sampling strategy presented above with a customized, low-input RNA-seq protocol based on single-cell tagged reverse transcription sequencing (STRT-seq) chemistry. (scienceboard.net)
Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)

Our results support the use of DNA label retention to identify MaSC and also provide a molecular profile and novel candidate markers for these cells. (frontiersin.org)
Veelken HWood GSSklar J Molecular staging of cutaneous T-cell lymphoma: evidence for systemic involvement in early disease. (jamanetwork.com)
Thus, we provide new evidence that the molecular phenotype of CIS cells is similar to that of gonocytes. (ku.dk)

The Applied Biosystems ArcturusXT is a microdissection system (LCM) that uses UV and IR lasers to capture the isolated cell without changing morphology or integrity of a biological tissue. (concordia.ca)
Cell Observer is a system to document your living cells for long term experiments with reproducible results, Document movement, shape and physiological processes in living cells. (antisel.gr)
HyperSpectral Karyotyping (SKY) of human and mouse cells with ASI Complete Multicolor Karyotyping & FISH System (HiSKY): ASI's HyperSpectral platform is based on Fourier Transform Spectroscopy. (weizmann.ac.il)
Small Scale Cell Based Screens: NC60 panel based small scale screens with high precision EzMate automated pipetting robotic system and Infinite Pro200 plate reader. (weizmann.ac.il)

Of these, single cell RT-qPCR provides combined advantages of sensitivity, specificity, and dynamic range, but is limited by low throughput, high reagent cost, and difficulties in accurately measuring low abundance transcripts (6). (justia.com)

BAL) samples yielded few infl ammatory cells. (cdc.gov)
Most surgically removed tissue samples contain a low percentage of tumor cells embedded in a dense stroma. (genengnews.com)
During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome. (leica-microsystems.com)
However, direct studies on purified samples of CIS cells are lacking. (ku.dk)
It is a contactless and contamination-free process for separating specific single cells or entire areas of tissue from a wide variety of tissue samples. (dssimage.com)
The QIAseq Single Cell DNA Library Kit UDI can support up to 384 samples in a single flow-cell lane, increasing sample throughput and lowering sequencing costs. (qiagen.com)
CellCelector gives a unique ability working with low amount cell samples. (weizmann.ac.il)

Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)
Therefore, we investigated whether pepsinogen C (PGC) transcript-expressing cells represent the cellular origin of metaplasia and cancer using a novel Pgc-specific CreERT2 recombinase mouse model. (bvsalud.org)
We further established Pgc-CreERT2;KrasG12D/+ mice and investigated whether PGC transcript-expressing cells are responsible for the precancerous state in gastric glands. (bvsalud.org)

Microdissection refers to a variety of techniques where a microscope is used to assist in dissection. (wikipedia.org)
Laser microdissection - use of a laser through a microscope to dissect selected cells. (wikipedia.org)
LCM uses a laser and a microscope to literally flip individual cells out of a tissue into a container. (the-scientist.com)
A micro-capillary single-cell based microscope is known as MMI CellEctor it helps in arranging systems for quick recognition, testimony, and taking up of single or rare cells in suspension. (dssimage.com)

BACKGROUND & AIMS: Metaplasia and dysplasia in the corpus are reportedly derived from de-differentiation of chief cells. (bvsalud.org)

Frozen sections of tissue and cytological specimens are also suitable for laser microdissection. (concordia.ca)

We profiled the transcriptome, chromatin accessibility state, and heteroplasmy in cells from the eyes of a patient with mitochondrial encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) and from healthy control donors. (jci.org)

Recent technological advances have accelerated single-cell analyses, but workflows remain expensive and complex. (nature.com)
The coordinates of the detected regions are stored in a data table which can be used for relocation of the male cells for further analyses. (ugent.be)

NCEZID scientists used a technique known as laser microdissection to pinpoint the specific cells where Zika virus might be persisting and replicating . (cdc.gov)

CTCs trapped by the chip also act as a "liquid biopsy" of the tumor, providing convenient access to tumor cells and earlier information about potentially fatal metastases. (sciencedaily.com)

To investigate the mechanisms of retinal iron regulation, the lab uses conditional knockout mouse models, human retinal tissue, and retinal cell tissue culture. (upenn.edu)
2)In retinal cell culture, study the effects of inflammatory mediators on retinal iron transporters. (upenn.edu)
Our study demonstrates a circadian rhythm of contrast sensitivity that peaks during the daytime, and that its regulation involves interactions of D4Rs, the clock gene Npas2 , and the clock-controlled gene adenylyl cyclase 1 ( Adcy1 ) in a subset of retinal ganglion cells (RGCs). (jneurosci.org)

And, we furtherly performed single-cell spatial transcriptomic sequencing on postnatal day 1 mouse enthesis, in order to deconvolute bone-tendon junction (BTJ) chondrocytes onto spatial spots. (elifesciences.org)
In this study, we investigated the transcriptomic changes in cortical neurones, and associated astrocytes and endothelial cells of the neurovascular unit, in the ageing brain. (biomedcentral.com)

Microdissection procedure: the upper part of the dermis (papillary dermis) is removed, along with the very lower part of the epidermis, with a 30-gauge needle under microscopic visual control (objective magnification ×40). (jamanetwork.com)

Circulating tumor cells, or CTCs, play a crucial role in cancer metastasis, spreading from tumors to other parts of the body, where they form new tumors. (sciencedaily.com)

Here, we identify nonrandom distribution of a pathogenic mtDNA variant across a complex tissue using single-cell RNA-Seq, mitochondrial single-cell ATAC sequencing, and multimodal single-cell sequencing. (jci.org)
The QIAseq Single Cell DNA Library Kit UDI includes protocols for unbiased whole genome amplification and for target enrichment of small genomes, such as viral DNA or mitochondrial genomes. (qiagen.com)
Seahorse Metabolic Analyzers service for rapid and quantitative measurement of cellular bioenergetics: Seahorse XF Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate to interrogate key cellular functions such as mitochondrial respiration and glycolysis. (weizmann.ac.il)

If we sample the cells every 15 minutes, it does not change cell either morphology or activity," he said. (genomeweb.com)
If [intervals are] less than 15 minutes then cells start changing morphology. (genomeweb.com)
However, usefulness of microdissection is often limited by the difficulties in identifying desired cell types by morphology alone. (genengnews.com)

It is a unique neoplasm in which the malignant cell, the Reed-Sternberg cell (RSC), represents only a small proportion of cells constituting the bulk of the tumor (see the image below). (medscape.com)
In adenocarcinomas, K17 staining was detected in a mean of 33.9% of malignant cells. (medscape.com)

High-throughput single cell measurement technologies are therefore of interest and have broad applications in clinical and research settings. (justia.com)

The] nanopipette technology that we have developed is capable of analyzing a single cell while [that] cell is alive," Nader Pourmand, professor of biomedical engineering at UCSC and the study's senior author said via email. (genomeweb.com)
Pourmand's lab had established the underlying method of sampling material from single cells while maintaining cell viability in 2014 and the more recent study represents a way to reliably do so at scale. (genomeweb.com)
The utility of longitudinal data in single-cell spatial transcriptomics is well known and numerous methods attempt to address it. (genomeweb.com)
Single-cell omics provide insight into cellular heterogeneity and function. (nature.com)
Single-cell sequencing has made considerable progress relying on the notion that the sequence is the tag. (nature.com)
[ 2 ] The origin of the HL Reed-Sternberg cells (HL-RSC) became clear with the introduction of microdissection and single-PCR methods. (medscape.com)
Microfluidic devices are provided for trapping, isolating, and processing single cells. (justia.com)
Single cells represent a fundamental biological unit. (justia.com)
In particular, microfluidic qPCR devices (Biomark Dynamic Array, Fluidigm) have recently been applied to single cell studies (15, 16). (justia.com)
We now can not only capture cancer cells from blood with high efficiency but also hand-pick single CTCs for in-depth characterization to provide crucial information that helps doctors make better decisions. (sciencedaily.com)
Using the new assay on patients' blood containing circulating melanoma cells (CMCs), Tseng's team was able to isolate and preserve single CMCs. (sciencedaily.com)
In comparison to PCR-based methods of whole genome amplification, the Multiple Displacement Amplification (MDA) technology included in the QIAseq Single Cell DNA Library Kit UDI eliminates PCR duplicates and minimizes GC-bias, ensuring high library diversity and maximum genome coverage. (qiagen.com)
This kit relies on three key technologies to deliver high-diversity whole genome libraries from single cells. (qiagen.com)
The entire process, beginning with an isolated single cell, is PCR-free and requires only 3.5 hours to complete. (qiagen.com)
The QIAseq Single Cell DNA Library Kit UDI is a complete cell-to-library solution that includes all the reagents necessary to generate high-diversity whole genome libraries from single cells or limited quantities of intact gDNA. (qiagen.com)
Large Scale Mammalian Cells and Cell clusters expansion plant: GE Wave bioreactor systems with single-use up to 25L capacity bags for suspension cells culture or microcarrier-based adherent cells culture. (weizmann.ac.il)

Metastasis results from the dissemination of cancer cells from a primary tumour to other anatomical sites and is the most common cause of death for lung cancer patients. (ucl.ac.uk)
Researchers have refined a method they previously developed for capturing and analyzing cancer cells that break away from patients' tumors and circulate in the blood. (sciencedaily.com)
Tseng's team now reports that they have improved the NanoVelcro chip by replacing its original non-transparent silicon nanowire substrate inside with a new type of transparent polymer nanofiber-deposited substrate, allowing the device's nanowires to better "grab" cancer cells as blood passes by them. (sciencedaily.com)

We are using an approach that would target cells within functional domains of the cochlea. (uhhospitals.org)
Laser capture microdissection: Sample here shows capture of cells from functional domains of the mouse cochlea. (uhhospitals.org)

The goals for advances in flow cytometry are clear: measure as many relevant target molecules per cell as quickly as possible. (nature.com)

A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy. (genomeweb.com)
Retention of labeled DNA strands may be attributed to the ability of stem cells to retain the parental DNA strand during asymmetric cell division ( Cairns, 1975 ) or to quiescence of the stem cell population such that the DNA label is not diluted by frequent cell divisions ( Klein and Simons, 2011 ). (frontiersin.org)
During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
This method allowed us to zero in on a small population of cancer stem cells expressing CD24, a specific cell surface biomarker shown to correlate with the development and metastasis of pancreatic cancer," continues Dr. Lubman. (genengnews.com)
We did not find indications that CIS was derived from a meiotic cell, and the similarity to embryonic stem cells was modest compared with gonocytes. (ku.dk)
However, how RUNX3R122C mutation disrupts stem cell homeostasis and promotes gastric carcinogenesis remained unclear. (bvsalud.org)
Mechanistically, RUNX3R122C mutant protein disrupted the regulation of the restriction point where cells decide to enter either a proliferative or quiescent state, thereby driving stem cell expansion and limiting the ability of cells to terminally differentiate. (bvsalud.org)

The soybean cyst nematode (SCN) is the major pest of soybean and causes hundreds of millions of dollars in losses each year in the U.S. The nematode feeds on soybean root cells. (usda.gov)
Syncytial cells in soybean (Glycine max cultivar [cv. (usda.gov)

Anatomy4

Diseases1

Chemicals and Drugs9

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes7

Disciplines and Occupations2

Information Science3

Health Care1

Laser Capture Microdissectio1

MRNA7

Populations8

Tissues11

Leica Microsystems2

Mutations4

Fetal8

Biology5

Enables2

20221

Genes15

ScRNA-seq2

Fluorescence4

Biomarkers1

Lysis4

Epithelial cells5

Progenitor cells5

Situ1

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Pathways4

Abnormal1

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Analysis17

Chromosome1

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Germ cell t2

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Analyze2

Genomic2

Microenvironment1

Isolation of Single Cells1

Mesenchymal stem1

Gene expression6

Molecular3

System4

Abundance1

Samples7

Transcript3

Microscope4

Differentiation1

Suitable1

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Analyses2

Scientists1

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Stem7

Soybean2