Neural protocols cells cultured. Medical search. Frequent questions

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. (wikipedia.org)
After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907 to 1910, establishing the methodology of tissue culture. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture. (wikipedia.org)
He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
In modern usage, "tissue culture" generally refers to the growth of cells from a tissue from a multicellular organism in vitro. (wikipedia.org)
More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. (nature.com)
Sandström J., Eggermann E., Charvet I., Roux A., Toni N ., Greggio C., Broyer A., Monnet-Tschudi F., Stoppini L. "Development and characterization of a human embryonic stem cell-derived 3D neural tissue model for neurotoxicity testing. (chuv.ch)
However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
CellAdhere™ Dilution Buffer and non-tissue culture-treated cultureware (e.g. (stemcell.com)
Transfer two milliliters of the liquid culture medium into each well of a 24 well tissue culture plate. (jove.com)
Take two colonies with an inoculating needle from the inoculated petri dishes in step, and place them into the first well of the tissue culture plate. (jove.com)
Incubate the tissue culture plate at 30 degrees Celsius for seven days. (jove.com)
Embryoid bodies were then plated on tissue-culture plastic dishes and cultured in serum-free, defined media (ITSFn). (nih.gov)
After 5-7 days selection in ITSFn, adherent cells were trypsinized, triturated to a single cell suspension, and re-plated on tissue culture dishes coated with 15 ug/ml poly-L-ornithine (Sigma) and 1 ug/ml human plasma fibronectin (Gibco). (nih.gov)
The results from a study that used cells derived from a deceased patient's skin tissue has recently been published. (psychiatrictimes.com)
2 It was reported that stem cells have an ability to transform themselves into different cell types, depending on the tissue, essentially at the scientist's whim. (psychiatrictimes.com)
The 4x and 10x objectives can obtain images of cells grown on standard tissue culture well plates. (sheffield.ac.uk)
Disassociates and magnetically sorts primary neural tissue. (universityofrochester.jobs)
Human organoids are small, self-organized, three-dimensional (3D) tissue cultures that have started to revolutionize medical science in terms of understanding disease, testing pharmacologically active compounds, and offering novel ways to treat disease. (mdpi.com)
They include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. (ac.ir)
The neuronal cultures are similar to nervous tissue in physiological aspects. (ac.ir)
However, significant challenges such as accessibility of macaque brain tissue and the lack of a robust neural cell isolation and culture protocol have hampered its progress. (springernature.com)
These properties provide stem cells with powerful capabilities for tissue repair, replacement, and regeneration, so human stem cells are of special interest in medical research. (sigmaaldrich.com)
The ability to expand pluripotent cells in vitro and subject them to direct differentiation to produce specific cell types is crucial to the development of cell-based therapies to replace or restore tissue that has been damaged by disease or injury. (sigmaaldrich.com)
Here, we identify nonrandom distribution of a pathogenic mtDNA variant across a complex tissue using single-cell RNA-Seq, mitochondrial single-cell ATAC sequencing, and multimodal single-cell sequencing. (jci.org)
Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)
In this project, involving engineers from LTH, we apply advanced microfluidic techniques to culture hESCs under the influence of chemical gradients to mimic the environment around the developing brain in the fetus, thereby generating neural tissue with anatomical characteristics resembling the developing human brain. (lu.se)
Countries where vaccines produced in neural tissue have not yet been replaced by cell culture and purified embryonated egg derived rabies vaccines will need to consider appropriate strategies for future rabies control. (who.int)

Neural input into this bioprinted skeletal muscle construct shows the improvement of myofiber formation, long-term survival, and neuromuscular junction formation in vitro. (nature.com)
Therefore, the amount of cholesterol available in vitro may be insufficient, particularly during cell proliferation, repair and maintenance of organ-specific functions (e.g. in cells of the nervous system during neurite extension, synaptogenesis, and myelination). (forschung3r.ch)
Furthermore, we have enriched the hNSCs for the cell surface marker CD133 via magnetic sorting, which has led to an increase in the expansion rate and neuronal fate specification of the hNSCs in vitro. (ca.gov)
It is unequivocal that membrane-presented ligand (delivered to target cells via cell-surface presentation by co-culture with ligand-expressing third-party cells) induces a greater extent of carcinoma cell death in vitro in comparison to non-cross-linked agonists (agonistic antibodies and/or recombinant ligands). (bio-thing.cn)
1394). 'Effects of different culture media on optimization of primary neuronal cell culture for in vitro models assay', , 9(3), pp. 163-170. (ac.ir)
We also utilized adult brain slicing protocols from in vitro patch-clamping, in which brains are sectioned within a neural protective recovery solution to enhance neural survival. (springernature.com)
We used a previously characterized in vitro differentiating neural stem cell (NSC) system to investigate the interplay between Mecp2 isoform-specific expression and DNA methylation at the Mecp2 REs. (biomedcentral.com)
Differentiation is triggered by various factors in vivo , some of which can be replicated in in vitro stem cell cultures. (sigmaaldrich.com)
These protocols, such as organoid cell culture methods, have provided more predictive in vitro cellular "Disease-in-a-Dish" models. (sigmaaldrich.com)
Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
During his PhD, he developed microfluidic devices and 3D printing methods for in vitro engineering of stem cell-based neural circuits. (lu.se)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

This 3D primary cell culture system prepared from embryonic rat brain cells (2-5) contains all types of brain cells (i.e., neurons, astrocytes, oligodendrocytes, and microglia) that are enabled to grow and mature in serum-free medium, and to form highly differentiated structures such as myelinated axons, synapses, and functional neuronal networks. (forschung3r.ch)
demonstrate through studies on induced pluripotent stem cell-derived cortical neurons that miR-33a is a potential therapeutic target for the treatment of SPG4-related hereditary spastic paraplegia. (portlandpress.com)
Induction of long-term potentiation (LTP) of the synaptic connection between two hippocampal glutamatergic neurons in a neural network formed in cell culture resulted in a specific pattern of potentiation at other connections within the network. (jneurosci.org)
In cultured hippocampal slices, LTP induced at one set of synaptic inputs to CA1 pyramidal neurons also spreads to nearby synapses on the same postsynaptic neuron ( Engert and Bonhoeffer, 1997 ), resulting in a "breakdown" of input specificity. (jneurosci.org)
In cultures of dissociated hippocampal neurons, induction of long-term depression (LTD) at glutamatergic synapses is accompanied by a backpropagation of depression to input synapses on the dendrites of the presynaptic neuron. (jneurosci.org)
In the present study, we used cultures of dissociated hippocampal neurons to characterize the spread of LTP in defined neural networks. (jneurosci.org)
Using perforated whole-cell patch clamp, we simultaneously monitored all synaptic connections within the networks of three or four neurons. (jneurosci.org)
The purpose of this study was to investigate PDT using the photosensitiser, meta tetrahydroxyphenyl chlorin (mTHPC), on rat neurons and satellite glia, compared with human adenocarcinoma cell (MCF-7). (open.ac.uk)
Conclusion: Neurons in culture can survive mTHPC-PDT under conditions sufficient to kill tumour cells and other nervous system cells. (open.ac.uk)
Gibco CultureOne Supplement may be added to any conventional neuronal differentiation medium to eliminate more than 75% of contaminating neural progenitor cells-resulting in superior neuronal cell cultures of evenly-distributed, differentiated neurons, enabling improved downstream assays, accelerated neuronal maturation, and seamless maintenance for 5 weeks or more. (thermofisher.com)
For primary neuron cultures, CultureOne Supplement reduces the number of proliferating glial progenitors which can overgrow post-mitotic neurons. (thermofisher.com)
CultureOne Supplement was developed to help support neuronal differentiation of human neural stem cells (NSCs) to neurons for maximum utility in downstream applications. (thermofisher.com)
After 2 weeks of neuronal differentiation with CultureOne Supplement, images show evenly distributed differentiated neurons (MAP2 + ) with greater than 75% reduction in NSCs (SOX1 + ) and cell clumps compared to conventional neuronal differentiation methods (A) . These cells had increased neuronal mRNA expression, reduced NSC mRNA expression (B) , and exhibited higher spike rates as measured by multi-electrode array (MEA). (thermofisher.com)
Neurons differentiated from NSCs with CultureOne Supplement showed an increase in cytosolic calcium when depolarized with KCl, meaning they express significantly higher numbers of voltage-gated calcium ion channels, an important marker for neural maturity and excitability. (thermofisher.com)
Primary rat cortical neurons and (B) mouse cortical neurons were cultured for 3 weeks In B-27 Plus Neuronal Culture System complete medium. (thermofisher.com)
Rat cortical neurons or mouse cortical neurons were plated on poly-d-lysine-coated plates and cultured in the B-27 Plus Neuronal Culture System. (thermofisher.com)
HCS Studio Cell Analysis Software was used to quantify the number of neurons and number of HuC/HuD-positive cells. (thermofisher.com)
Here, we show that the protein level of ClpP is selectively decreased in αSyn-expressing cell culture and neurons derived from iPS cells of PD patient carrying αSyn A53T mutant, and in dopaminergic (DA) neurons of αSyn A53T mice and PD patient postmortem brains. (springer.com)
Overexpression of ClpP reduces αSyn-induced mitochondrial oxidative stress through enhancing the level of Superoxide Dismutase-2 (SOD2), and suppresses the accumulation of αSyn S129 phosphorylation and promotes neuronal morphology in neurons derived from PD patient iPS cells carrying αSyn A53T mutant. (springer.com)
It involves answering some simple questions: What if you took the skin cells from patients who had schizophrenia and turned them into neurons? (psychiatrictimes.com)
The directed differentiation of induced pluripotent stem cells (iPSC) into specific neuronal subtypes provides an unlimited supply of human neurons that can be used to study disease mechanisms. (researchsquare.com)
In the literature, there are different methods for the isolation and culture of rat embryonic cortical neurons. (ac.ir)
Importantly, the major advantage of this protocol is that our approach is able to yield viable and high-density neural progenitor cells (NPCs), neurons, and immature cells (OPCs, immature astrocytes and immature granule cells). (springernature.com)
In addition, our primary cell isolation protocol was adapted for high-yield neurons. (springernature.com)
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture. (sigmaaldrich.com)
ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes. (sigmaaldrich.com)
During vertebrate development, distinct classes of neurons arise at stereotypical locations along the dorsoventral axis of the spinal neural tube. (silverchair.com)
CRT aims to replace neurons that have degenerated in PD, with donor cells that have the potential to functionally re-integrate into the host circuitry. (lu.se)
This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)
In line with this aim, we also perform extensive preclinical validation of the human dopamine neurons derived from our protocol in rat models of Parkinson's disease. (lu.se)
However, with a growing number of novel cell sources (stem cells and reprogrammed cells) it becomes important to develop new methodologies for a more thorough assessment of human neurons generated using these approaches. (lu.se)
We utilise monosynaptic tracing technology, using pseudotyped rabies vectors, in order to visualise the synaptic contacts formed between host neurons and graft human cells. (lu.se)
The cells are called induced neurons (iNs) and offer a shortcut over induced pluripotent stem cells (iPSc) for generating patient and disease-specific neurons. (lu.se)
Current efforts focus on identifying the optimal starting cell population for neural conversion, identification of gene combinations that yield subtype specificity and analysis of function and integration of the reprogrammed neurons. (lu.se)
Ewing sarcomas are thought to derive from cells of the neural crest, possibly mesenchymal stem cells, via a pathway that might include postganglionic cholinergic neurons. (medscape.com)
After joining Parmar lab, he has been working on direct reprogramming of adult human fibroblasts into dopaminergic neurons in injectible 3D cultures as a potential route towards patient-specific cell-replacement therapy for Parkinson's disease. (lu.se)

The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
Epithelial to mesenchymal transition during mammalian neural crest cell delamination. (stowers.org)
Information about mesenchyme, specifically mesenchymal stem cell procotols. (sigmaaldrich.com)
Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation. (sigmaaldrich.com)
Combining our CRISPR, ZFN gene editing , and stem cell expertise, we now offer novel stem cell lines, optimized media, and innovative kits for all areas of stem cell biology, including induced pluripotent stem cells (iPSCs), neural, mesenchymal and hematopoietic stem cell culture. (sigmaaldrich.com)
Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)

Support the growth and differentiation of human pluripotent stem cells by using Vitronectin XF™, a defined and xeno-free cell culture matrix. (stemcell.com)
Use Vitronectin XF™ with mTeSR™1 , mTeSR™ Plus , TeSR™-E8™ , or TeSR™-AOF medium to provide a defined culture system for the maintenance of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells under feeder-free conditions. (stemcell.com)
STEMdiff™ Neural Progenitor Medium is a defined and serum-free medium for the expansion of neural progenitor cells (NPCs) derived from human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium (Catalog #05835). (stemcell.com)
Murine V6.5 ES cells were expanded on Mitomycin-C-inactivated MEF feeder layers in pluripotent media supplemented with LIF. (nih.gov)
I'll start with a brief review of hiPSCs (human inducible pluripotent stem cells), then move to the data. (psychiatrictimes.com)
An article published that month from a group in Kyoto, Japan, described how to take garden-variety mouse skin cells and turn them into pluripotent stem cells. (psychiatrictimes.com)
The lab employs a diverse range of model systems including patient-derived induced pluripotent stem cells (iPSCs), neuronal cultures and mouse models. (purdue.edu)
Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into forebrain neural progenitor cells. (lu.se)
Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing. (sigmaaldrich.com)
This article provides an extensive step-wise overview of CRISPR Cas9 protocol that can be used to perform gene editing in human induced pluripotent stem cells (iPSCs). (sigmaaldrich.com)
Pluripotent stem cells, including embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny representative of all three germ layers (ectoderm, endoderm, and mesoderm). (sigmaaldrich.com)
Directs a variety of small, complex research projects related to the culture of both primary neural cells and pluripotent stem cells. (universityofrochester.jobs)
Independently prepares both primary neural cells and the derivatives of pluripotent stem cell lines for fluorescence-activated single cell sorting (FACS) and magnetic-activated cell separation (MACS) analysis, as well as sorting, to meet the specific needs of lab research projects. (universityofrochester.jobs)
No evidence of tumour formation was observed in the transplanted animals, a major concern when utilising a pluripotent cell source. (lu.se)
Using this type of direct conversion approach rather than making them from induced pluripotent stem cells, where they revert into a naïve state, helps to retain the epigenetic age of the patient in the generated interneurons. (lu.se)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph. (wikipedia.org)

Neural Progenitor Cells Derived from XCL-1 DCXp-GFP are a neuronal lineage reporter cell line that can be used in neuronal differentiation and drug screening. (atcc.org)
To download a certificate of analysis for Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
The certificate of analysis for that lot of Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ) is not currently available online. (atcc.org)

Also, a vascularization strategy using co-culture with endothelial cells (ECs) and fibroblasts improved the survival of the bioengineered skeletal muscle tissues 20 , 21 . (nature.com)
1) One is to replace serum by other biological but as yet inadequately defined mixtures such as extracts from plants, animal tissues or cells. (forschung3r.ch)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Laser scanning confocal microscopy is a significant advance in the field of optical microscopy, primarily because it permits sample visualization deeper within living and fixed cells, tissues and other samples. (sheffield.ac.uk)
Although it is expressed broadly, FKBP8 is required to antagonize SHH signaling primarily in neural tissues, suggesting that hedgehog signal transduction is subject to cell-type specific modulation during mammalian development. (silverchair.com)
The wide range of manifestations observed in patients with mitochondrial disease results from varying fractions of abnormal mtDNA molecules in different cells and tissues, a phenomenon termed heteroplasmy. (jci.org)
However, the landscape of heteroplasmy across cell types within tissues and its influence on phenotype expression in affected patients remains largely unexplored. (jci.org)
Tissues and Cells. (lu.se)
The revised recommendations for biosafety in production and quality control areas for rabies vaccines do not cover those produced in mammalian neural tissues. (who.int)
Moreover, there is evidence for a lack of potency of certain vaccines produced in neural tissues, leading to inadequate protection in humans. (who.int)
In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)

In his present investigation he used this serum-free aggregating brain cell cultures as a reference culture system. (forschung3r.ch)
Another is to adapt useful cell lines individually for survival and growth in serum-free culture media. (forschung3r.ch)
mESCs were maintained in a serum free condition with CHIR99021, PD184352, and LIF on sparse feeder cells. (nih.gov)
In addition to our expansive portfolio of assay-ready stem cells, serum-free cell culture media, and 3D culture solutions, we offer custom engineered stem cell lines through our easy-to-use Cell Design Studio. (sigmaaldrich.com)

Neural progenitors were derived by depleting feeder cells and culturing cells in suspension in neural differentiation basal medium (DFNB) for 2 days followed by 500nM all-trans retinoic acid treatment in DFNB. (nih.gov)

The lab has been applying iPSC technology to neurotoxicity research for 13+ years, and has established protocols to generate iPSC lines and differentiate them down forebrain, striatal and midbrain neural lineages. (purdue.edu)
Furthermore, we have used this protocol to differentiate PSCs from humans and chimpanzees. (lu.se)
We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)
Stem cells have the unique ability to self-renew or to differentiate into various cell types in response to appropriate signals. (sigmaaldrich.com)
Embryonic stem cells have the ability to differentiate into more cell types than adult stem cells. (sigmaaldrich.com)
The identification of this second cell population helps differentiate MNTI from other small, round, blue cell tumors. (medscape.com)

After reverse transcription and template switching, we amplified cDNA with KAPA HotStart HIFI 2× ReadyMix (Kapa Biosystems) for 22 cycles for RNA from single primary cortical cells. (nih.gov)
Primary cortical cultures neurite outgrowth and neuron numbers per field of common mediums were compared for neuronal cells isolation and expansion. (ac.ir)

In our recent work published on Nature Protocols, we describe a simple and reproducible method for the isolation and culture of functional adult and aged macaque brain cells. (springernature.com)
Isolation of primary cells from the hippocampus provides an opportunity to study neurogenesis, particularly capturing the neurogenesis process. (springernature.com)

A) Typical NPC morphology is observed in cultures (shown at day 6 of passage 1). (stemcell.com)
Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)

The ability to characterize and study neural cells isolated directly from the macaque brain would greatly promote the research in human neuroscience. (springernature.com)
Members of the frog Xenopus genus are versatile model organisms used in developmental biology, cell biology, neuroscience, and toxicology research, among others. (cshlpress.com)

The cells are formally termed "iPSCs. (psychiatrictimes.com)
We offer a large collection of cell culture media, supplements, bioactive small molecules, and growth factors used to control the cell fate of human iPSCs. (sigmaaldrich.com)

NPCs cultured in this medium can be expanded 3-5 fold per passage, and cultured for at least 10 passages, with minimal spontaneous neuronal differentiation. (stemcell.com)

It is an important building block of all cell membranes, and an important metabolite in many biosynthetic pathways. (forschung3r.ch)
Such selective propagation suggests the existence of a long-range cytoplasmic signaling within the presynaptic neuron, leading to a specific pattern of coordinated potentiation along excitatory pathways in a neural network. (jneurosci.org)
It enables the scrutinization of intracellular signaling pathways responsible for cell death. (ac.ir)
Identification of Inflammatory, Metabolic, and Cell Survival Pathways Contributing to Cerebral Small Vessel Disease by Postmortem Gene Expression Microarray. (unibas.ch)

This system allows complete control over the culture environment, resulting in more consistent cell populations and reproducible results in downstream applications. (stemcell.com)

Although human HSCs as vehicles to treat metachromatic leukodystrophy (MLD) has been used to treat patients with early onset MLD in a phase I/II trial, the HSCs give rise to all different blood cell lineages, such as the myeloid and lymphoid cell lineages [ 11 ]. (hindawi.com)
their evolution from distinct cell lineages. (lu.se)

Loss of GPR56 does not affect cell proliferation, migration, or neurite outgrowth. (jneurosci.org)
In some cases, neuron survival was confirmed by measuring subsequent neurite growth in monolayer culture. (open.ac.uk)
Neuronal cell cultures differentiated with CultureOne Supplement were compared to anti-mitotic enrichment methods including Ara-C and mitomycin C. CultureOne Supplement-treated cultures exhibited improved neurite outgrowth and reduced cell clumping and death. (thermofisher.com)

We found that potentiation propagated from the site of induction retrogradely to glutamatergic or GABAergic synapses received by the dendrites of the presynaptic neuron and laterally to those made by its axonal collaterals onto other glutamatergic cells. (jneurosci.org)
In contrast, synapses made by the same presynaptic neuron onto GABAergic cells were not affected, and there was no postsynaptic lateral or forward propagation to other synapses received or made by the postsynaptic neuron. (jneurosci.org)
These results imply the existence of a long-range cytoplasmic signaling within the presynaptic neuron and add a new dimension to activity-induced synaptic modification at the network level that bears direct implications to developmental remodeling and learning functions of the neural network. (jneurosci.org)
Importantly, 4 µg ml -1 mTHPC PDT caused no significant neuron death compared with untreated controls but was sufficient to elicit substantial cell death in the other cell types. (open.ac.uk)
The protocol was validated using hypericin (0-3 µg ml -1 ), which caused neuron death equivalent to other cell types. (open.ac.uk)
This protocol resulted in an increased number of NPCs and immature cells from the hippocampus, which revealed the primate-specific physiological properties of the neuron populations and the molecular cascade during adult neurogenesis. (springernature.com)
I also describe the development of a novel behavioural task that is predictive of mesDA neuron cell loss in mice. (lu.se)

Sanchez-Pascual I., O'Laoi R., El M'Ghari I., Kocher-Braissant J., Ellisman MH, Toni N . "Fine processes of Nestin-GFP positive radial glia-like stem cells in the adult dentate gyrus ensheathe local synapses and vasculature. (chuv.ch)
Gebara E., Bonaguidi M., Beckervordersandforth R., Sultan S., Udry F., Gijs P., Lie C., Ming G., Song H., Toni N . "Morpho-functional characterization of adult neural hippocampal stem cells reveals two morphotypes of radial glia-like cells. (chuv.ch)
Braun S., Moss J., Toni N. , Jessberger S. "Programming hippocampal neural stem/progenitor cells into oligodendrocytes enhances remyelination in the adult brain after injury" Cell Reports , 2015 Jun 23;11(11):1679-85. (chuv.ch)
In the April issue of Psychiatric Times , I discussed how adult stem cells were being used to aid in research on a neurological disorder (spinal muscular atrophy). (psychiatrictimes.com)
The ability to interrogate neural cell from adult primates will greatly advance our understanding of the primate brain, especially with evidence to suggest that many phenotypic and functional differences exist between the primate brain and rodent model systems. (springernature.com)
To achieve high-yield and viability neural cells from adult and aged macaques, we have incorporated knowledge accumulated from multiple fields. (springernature.com)
When combined with single-cell profiling techniques, the approach allows an unbiased and comprehensive mapping of cell states in the adult and aged macaque brain, which is needed to advance our understanding of human cognitive and neurological diseases. (springernature.com)
To further confirm the dissociation of NPCs from the hippocampus of adult macaques, we have undertaken studies on culture of NPCs from adult macaque hippocampus. (springernature.com)
Furthermore, our work on primary hippocampal cells from adult macaque has been applied to compare the species discrepancies for NPCs. (springernature.com)
Studies using Xenopus cell-free extracts, oocytes, eggs, embryos, larval stages, and adult frogs have yielded important insights into a multitude of key biological processes-from mechanisms underlying the cell cycle to embryonic development to human disease. (cshlpress.com)
There are protocols for analyses of Xenopus immunology, neurobiology, metamorphosis, and adult physiology. (cshlpress.com)
Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. (lu.se)
This study provided important proof-of-principle that direct neural conversion of endogenous glia can take place in the adult rodent brain. (lu.se)
One way we did this was by converting adult fibroblasts, or skin cells, into interneurons. (lu.se)

Abstract] The search for the origin of the first hematopoietic stem cells (HSCs) in the mouse embryo has been a hot topic in the field of developmental hematopoiesis. (bio-thing.cn)

How to find reproducible culture conditions? (forschung3r.ch)
Written in the highly successful Methods in Molecular Biology™ , chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. (dattanibookagency.in)

Both induction and propagation of LTP required correlated spiking of the postsynaptic cell as well as the activation of the NMDA subtype of glutamate receptors. (jneurosci.org)
Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)

To generate single cell suspensions, hESC-derived cultures were dissociated from plates using Accutase (ThermoFisher) at 37°C. Light trituration using a P1000 pipette was done every 5 min until nearly all clumps had been dissociated (up to 1 h). (nih.gov)
Single-cell suspensions were loaded onto a FACSAria II SORP (Becton Dickinson) and sorted directly into PCR strip tubes or plates held in chilled aluminum blocks. (nih.gov)

In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. (nature.com)
The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)
This includes long-term survival of transplanted cells in vivo to assess cell fate, maturation and integration, as well as functional behavioural assessment for graft function in vivo. (lu.se)

Critically, we have also confirmed neural lineage specificity upon sorted hNSC transplantation into the immunodeficient NOD-scid mouse brain. (ca.gov)
NfsB is a dimeric flavoprotein enzyme that has broad electron acceptor specificity 15 and has been used in conjunction with prodrugs to selectively ablate cells in vertebrates. (cdc.gov)

Cell culture methods often lead to the reduction of animals used for biological research and industrial applications. (forschung3r.ch)
Common methods for the generation of human embryonic-derived neural stem cells (hNSCs) result in cells with potentially compromised safety profiles due to maintenance of cells in conditions containing non-human proteins (e.g. in bovine serum or on mouse fibroblast feeders). (ca.gov)
Methods: Fluorescence microscopy confirmed that mTHPC was incorporated into all three cell types. (open.ac.uk)
Collaborates with investigators in writing protocols for the conduct of research which include: purposes of study, design of experiments, lists of materials and methods to be used and the schedule of activities. (universityofrochester.jobs)
To circumvent these obstacles, we have developed two methods for the investigation of human neural cells in culture. (lu.se)
We also work on more sophisticated 3D culturing methods to model human brain development on an anatomical level with hESCs. (lu.se)

Through cell survival-based cDNA expression screens in neural progenitor cells, we identify a genetic variant of AGS Atp5g1 that confers cell resilience to metabolic stress. (elifesciences.org)
Ectopic expression in mouse cells and CRISPR/Cas9 base editing of endogenous AGS loci revealed causal roles of one AGS-specific amino acid substitution in mediating cytoprotection by AGS ATP5G1. (elifesciences.org)
At the age of defect onset, GPR56 expression is restricted specifically to developing granule cells in the rostral cerebellum, suggesting that GPR56 regulates properties of these cells. (jneurosci.org)
this limitation has been overcome via ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, to produce large quantities of these cells as an attractive source for cellular transplantation [ 16 - 18 ]. (hindawi.com)
A good understanding of cell and molecular biology and hands-on experience in mammalian cell culture and with experience in DNA cloning and expression is required. (universityofrochester.jobs)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)

Prepares written reports and presentations on all on-going projects, consisting of photomicrographs and statistical analyses of cell counts, which are used in the publication of articles. (universityofrochester.jobs)

However, the first B-lymphoid potential in the mouse embryos are reported to be biased to innate-like B-1 cell lineage that can develop from hemogenic endothelial cells (HECs) independently of HSCs. (bio-thing.cn)
Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)

Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
Interference RNA-mediated knockdown of GPR56 recapitulates the loss of adhesion seen in knock-outs, and reexpression of GPR56 rescues the adhesion defect in knock-out granule cells. (jneurosci.org)
Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
These results support a novel hypothesis that the cerebellar defects in Gpr56 knock-outs, and potentially in human patients with GPR56 mutations, arise from the disrupted adhesion of developing granule cells to the pial BM. (jneurosci.org)

These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. (wikipedia.org)
In the organism, liposoluble nutrients such as cholesterol, fatty acids and lipophilic vitamins reach the cells by means of specific apolipoproteins which form complexes with the lipids, the so-called lipoproteins (LPs), and which recognize specific membrane receptors expressed by the cells. (forschung3r.ch)
11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)

Prof. Dr. Paul Honegger and his group at the University of Lausanne developed aggregating cultures of mechanically dissociated primary fetal brain cells which provide an excellent system for neurobiological studies of cellular growth and differentiation. (forschung3r.ch)
Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. (dattanibookagency.in)
Experience on primary neural cell culture is a plus. (universityofrochester.jobs)

Neural cell dissociation protocols have been conducted in rodent, which ensures a quick process and a high yield. (springernature.com)
A cocktail of small-molecule inhibitors is added during perfusion and dissociation to protect neural cells from cytotoxicity by blocking receptors or channels. (springernature.com)

NYSCF is a preferred partner for gold-standard, automated generation of iPSC lines and derived cell types, in academic, non-profit, government and industry collaborations. (nyscf.org)

To reduce this heterogeneity, we generated engineered cerebral organoids (enCORs), which use a floating scaffold to increase the efficiency of neural induction and reduce heterogeneity. (researchsquare.com)
POLR1A variants underlie phenotypic heterogeneity in craniofacial, neural, and cardiac anomalies. (stowers.org)

B-D) NPCs maintained in STEMdiff™ Neural Progenitor Medium express the CNS-type NPC markers PAX6 (B, D, red), SOX1 (C, red) and NESTIN (C, green), but not the neural crest marker SOX10 (D, green, single channel shown in inset). (stemcell.com)

The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)

The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival. (wikipedia.org)

Studies have increasingly focused on the potential therapeutic effects of stem cell transplantation for neurological diseases [ 10 ]. (hindawi.com)
Ensures that cell and animal availability are coordinated for transplantation. (universityofrochester.jobs)
Maintains cells in culture and prepares cells for transplantation. (universityofrochester.jobs)
We still do not know what specific factors contribute to the success in transplantation i.e. what cells are responsible for motor recovery? (lu.se)
We monitor our cells after transplantation using standard in vivo assessments as listed above. (lu.se)

cDNA libraries were made using the NEBNext Small Library Prep Kit according to the manufacturer's protocol, except Superscript III was used for the RT step. (nih.gov)

After initial expansion, ES cells were passaged 2-3 times on 0.1% gelatin to remove contaminating feeder cells. (nih.gov)

Some stem cell lines are immortal and can be cultured indefinitely in the lab, but many are not. (sigmaaldrich.com)

His lab utilizes a combined approach of molecular genetics, pharmacology, biochemistry, cell and developmental biology to understand the role of gene-environment interactions between metal exposure and neurodevelopmental and neurodegenerative diseases including Parkinson's Disease, Alzheimer's Disease and Huntington's Disease. (purdue.edu)
Molecular biology of the cell, 27 (10). (unibas.ch)
Development: For advances in developmental biology and stem cells. (lu.se)
Frontiers in cell and developmental biology. (lu.se)
Combining cell biology and electrophysiology, his work has the potential to create personalized disease models for future research. (lu.se)

Recent experimental efforts suggest that astrocytes, a type of glial cell, are also directly involved in core cognitive processes such as learning and memory. (bvsalud.org)
Astrocytes are the most abundant cells in the nervous system. (bvsalud.org)
Compelling evidence for an involvement of astrocytes in potential mechanisms of DBS derive from studies suggesting that pharmacological lesions or the inhibition of these cells abolishes the antidepressant-like effect of DBS. (bvsalud.org)

V6.5 ES cells were differentiated into neural progenitor cells (NPCs) using established techniques (Meissner et al. (nih.gov)
Processed data file includes information for 2 replicates of ES cells and 2 replicates of ES-derived NPCs (ES_Rep1, ES_Rep2, NPC_Rep1, and NPC_Rep2). (nih.gov)
Our protocol can be achieved at different stages and state NPCs (RGL, IPC and NB). (springernature.com)

Here, we created a robust protocol that can be used later on for disease modeling of developmental or more early-onset brain disorders," explains Andreas Bruzelius. (lu.se)

Previous work showed that this culture system was ideal for the study of hormones, growth factors and other signaling molecules on cellular growth, maturation and homeostasis. (forschung3r.ch)

Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. (wikipedia.org)
Cell suspension was washed and filtered through a 40 μm cell strainer. (nih.gov)

The construction of this cell line is the first promising step in the regulation of GAL secretion from hTERT-immortalized BMSCs, and the potential application of this system may provide a stem cell-based research platform for pain. (hindawi.com)

Anatomy6

Organisms6

Diseases9

Chemicals and Drugs34

Analytical, Diagnostic and Therapeutic Techniques and Equipment124

Psychiatry and Psychology4

Phenomena and Processes32

Disciplines and Occupations7

Anthropology, Education, Sociology and Social Phenomena4

Technology, Industry, Agriculture3

Humanities3

Information Science26

Named Groups1

Health Care72

Geographicals6

Tissue37

Vitro12

Neurons37

Mesenchymal7

Pluripotent15

Embryonic cells1

Progenitor Cells Derived3

Tissues11

Serum-free4

Progenitors1

Differentiate6

Cortical2

Isolation2

Morphology2

Neuroscience2

IPSCs2

Neuronal differentiation1

Pathways4

Downstream1

Lineages2

Neurite3

Neuron7

Adult14

Hematopoietic1

Reproducible2

Subtype2

Single cell suspensions2

Functional3

Specificity2

Methods6

Expression7

Analyses1

Lineage2

Granule cells4

Organism3

Primary3

Dissociation2

IPSC lines1

Heterogeneity2

Crest1

Genetically2

Survival1

Transplantation5

CDNA1

Feeder cells1

Indefinitely1

Biology5

Astrocytes3

NPCs3

Robust1

Growth factors1

Suspension2

Stem cell-based1