• Neural crest stem cells (NCSCs) are multipotent and represent a valuable system to investigate iPSC differentiation and therapeutic potential. (ca.gov)
  • The differentiation of iPSCs and the expansion of derived NCSCs varied in different cell lines, but all NCSC lines were capable of differentiating into mesodermal and ectodermal lineages, including neural cells. (ca.gov)
  • We found that differentiating induced pluripotent stem cells (iPSC) to DA neuron progenitors followed by transplantation into the rat brain may enable differentiation into DA neurons. (suny.edu)
  • In this study, we used iPSC-derived neural progenitor cells (NPCs) from individuals with ASD of known and unknown genetic cause as well as from neurotypical controls to examine the effects of exogenous IL-17a on NPC proliferation, migration and neuronal differentiation, and whether IL-17a and genetic risk factors for ASD interact exacerbating alterations in NPC function. (figshare.com)
  • However, that they do not faithfully mirror all the differentiation capabilities of embryonic cells suggests that there are unknown factors at play that may limit their use in terms of modeling disease in the laboratory, one of the most important potential early applications of stem cell technology. (scienceblog.com)
  • The directed differentiation of induced pluripotent stem cells (iPSC) into specific neuronal subtypes provides an unlimited supply of human neurons that can be used to study disease mechanisms. (researchsquare.com)
  • Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
  • Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
  • Neural stem cells (NSCs) are undifferentiated neural cells with the capacity for long-term self-renewal and for differentiation into all types of neuronal and glial cells. (sanbio.nl)
  • The group also noted that establishing a standardized set of procedures for iPS cell derivation and differentiation would help ensure a robust resource and simplify the process of data sharing. (sfari.org)
  • Etoc noted the emphasis that RUMI Scientific has placed on micropatterning technologies 3,4 , which can standardize stem cell differentiation and the geometry of organoids by providing a lattice often comprised of extracellular matrix components. (sfari.org)
  • The Draviam lab aims to understand the molecular principles that govern cell division and the consequence of its failure when cells transition between states during differentiation. (qmul.ac.uk)
  • Together with MSD, the project aims to focus on developing AI methods to track iPSC (induced Pluripotent Stem Cells) differentiation to neural precursors, astrocytes and neurons. (qmul.ac.uk)
  • During the process of differentiation, quiescence and senescence are two cell dormancy states with distinct cell fates and transcriptomic statuses. (qmul.ac.uk)
  • The ratio of L-type/T-type Cav channel expression is increased in FXS progenitors and correlates with enhanced progenitor differentiation to glutamate-responsive cells. (neurosciencenews.com)
  • They traced this effect to the premature differentiation of neural stem cells inside the microcephalic tissue chunks, depleting the population of progenitor cells that fuels normal brain growth. (cbc-network.org)
  • Variation of DNA methylation on the IRX1/2 genes is responsible for the neural differentiation propensity in human induced pluripotent stem cells. (reprocell.com)
  • Pancreatic Differentiation of Stem Cells Reveals Pathogenesis of a Syndrome of Ketosis-Prone Diabetes. (reprocell.com)
  • We recently showed that some human induced pluripotent stem cell (iPSC) clones were defective in neural differentiation and were marked with the activation of long term repeats (LTRs) of human endogenous retroviruses (HERVs). (nih.gov)
  • The forced expression of KLF4 in human embryonic stem cells led to the activation of LTRs and defects in neural differentiation. (nih.gov)
  • Human induced pluripotent stem cells (hiPSCs) are a powerful model of neural differentiation and maturation. (bvsalud.org)
  • We present a hiPSC transcriptomics resource on corticogenesis from 5 iPSC donor and 13 subclonal lines across 9 time points over 5 broad conditions self -renewal, early neuronal differentiation, neural precursor cells (NPCs), assembled rosettes, and differentiated neuronal cells . (bvsalud.org)
  • Through the use of human embryonic stem cells (hESCs), we can mimic brain development towards different regions of the human brain, and thereby investigate the effect of novel genes on neural differentiation. (lu.se)
  • Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into forebrain neural progenitor cells. (lu.se)
  • I've also worked with differentiation from embryonic stem cells into interneurons. (lu.se)
  • Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
  • Induced pluripotent stem cells (iPSCs) hold great promise for cell therapies and tissue engineering. (ca.gov)
  • Here we derived NCSCs from human iPSCs and embryonic stem cells (ESCs), and investigated the potential of NCSCs for neural tissue engineering. (ca.gov)
  • and (b) leucine-rich repeat kinase 2 (LRRK2) gene identified patient induced pluripotent stem cells (iPSCs), which were used to isolate EMVs and begin to characterize their cargoes. (nih.gov)
  • Because analyzing actual human brain development is practically impossible, she uses human neural progenitor cells derived from induced pluripotent stem cells (iPSCs) as a model system. (the-scientist.com)
  • Partnering with scientists at Florida State University who study Zika virus, Ming's team inoculated the neural progenitors as well as human iPSCs, embryonic stem cells and immature neurons and compared the extent of the resulting infections. (the-scientist.com)
  • While less than 20 percent of the iPSCs, embryonic stem cells and neurons became infected, the infection of the neural progenitors was "really striking," said Ming. (the-scientist.com)
  • To gain a better understanding of potential disease mechanism, Ming's team plans to examine whether the infected neural progenitor cells lead to abnormal development of brain organoids-cultured mini brains derived from human iPSCs. (the-scientist.com)
  • NPCs can be differentiated from embryonic stem cells (ESCs) but also from induced pluripotent stem cells (iPSCs) (PMID: 11731782). (sanbio.nl)
  • My doctoral work aimed to unravel the mechanisms behind different pluripotency states in mouse, dog and human and their evolutionary significance using induced pluripotent stem cells (iPSCs) as models. (ncbs.res.in)
  • Indeed, if every disease known to man were cured by IPSCs or adult stem cells, the scientists would shrug and keep on cloning. (cbc-network.org)
  • METHODS: Here, we have carried out a longitudinal analysis of the transcriptome of human iPSCs undergoing neural induction. (ox.ac.uk)
  • This dynamical analysis of transcriptional changes provides a unique perspective of the widespread remodelling of the cell machinery that occurs during neural induction of human iPSCs. (ox.ac.uk)
  • When the generation of iPSCs was completed, LTRs were re-suppressed to levels similar to those in human ES cells. (nih.gov)
  • In an attempt to answer that, they used induced pluripotent stem cells (iPSCs) to generate neural progenitors from both humans and chimpanzees, which is our closest living relative in evolutionary terms. (lu.se)
  • Zika virus (green) infects human neural progenitors and leads to cell death (red). (the-scientist.com)
  • Ming and colleagues showed that infection of the progenitors either killed the cells or slowed their proliferation significantly. (the-scientist.com)
  • Neural progenitors "give rise to the larger population of neurons and glial cells of the brain," Ming said. (the-scientist.com)
  • What are neural stem cells (NSCs), neural progenitors, and neural precursors (NPCs)? (sanbio.nl)
  • Symmetric division of NSCs maintains the population of undifferentiated NSCs, while asymmetric division gives rise to neural progenitors. (sanbio.nl)
  • Neural progenitors are precursor cells able to further differentiate into various neuronal and glial cells. (sanbio.nl)
  • NSCs and neural progenitors are often regarded as neural precursors (NPCs). (sanbio.nl)
  • In a study recently published in the Stem Cell Reports journal, researchers from the University of Helsinki examined molecular mechanisms leading to disturbed neuronal network function in autistic spectrum disorders by utilising patient-specific neuronal progenitors differentiated from stem cells induced from blood or fibroblasts of skin. (neurosciencenews.com)
  • Open access research for "Increased Calcium Influx through L-type Calcium Channels in Human and Mouse Neural Progenitors Lacking Fragile X Mental Retardation Protein" by Claudia Danesi, Venkat Swaroop Achuta, Padraic Corcoran, Ulla-Kaisa Peteri, Giorgio Turconi, Nobuaki Matsui, Ilyas Albayrak, Veronika Rezov, Anders Isaksson, and Maija L. Castrén in Stem Cell Reports . (neurosciencenews.com)
  • We show that intracellular calcium responses to depolarization are augmented in neural progenitors derived from human induced pluripotent stem cells and mouse brain with FXS. (neurosciencenews.com)
  • Exogenous IL-17a did not induce abnormal proliferation and migration of ASD and control NPCs but, on the other hand, it significantly increased the expression of synaptic (Synaptophysin-1, Synapsin-1) and neuronal polarity (MAP2) proteins in these cells. (figshare.com)
  • Overexpression of ClpP reduces αSyn-induced mitochondrial oxidative stress through enhancing the level of Superoxide Dismutase-2 (SOD2), and suppresses the accumulation of αSyn S129 phosphorylation and promotes neuronal morphology in neurons derived from PD patient iPS cells carrying αSyn A53T mutant. (springer.com)
  • Our findings provide novel insights into the mechanism underlying αSyn-induced neuronal pathology, and they suggest that ClpP might be a useful therapeutic target for PD and other synucleinopathies. (springer.com)
  • We lastly adapt a previously generated RNA deconvolution approach to single- cell expression data to estimate the relative neuronal maturity of iPSC-derived neuronal cultures and human brain tissue . (bvsalud.org)
  • For gene therapy, a combinatorial approach with multiple target genes, as well as more complex optogenetic approaches are studied, while for cell-therapy human induced pluripotent stem cells and human induced neuronal cells are investigated. (lu.se)
  • Intracerebral grafting of neuronal cell suspensions II. (lu.se)
  • Our group previously reported low-Affinity nerve growth factor receptor (LNGFR)-and thymocyte antigen-1 (THY-1)-positive neural crest-like cells (LT-NCLCs), generated from human induced pluripotent stem cells (hiPSCs). (elsevierpure.com)
  • While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
  • Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
  • We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a robotic liquid handling unit that delivers footprint-free hiPSCs within 3 weeks with state-of-the-art efficiencies. (frontiersin.org)
  • Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
  • The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
  • Our objective is to produce a cell-based test for LQTS with induced pluripotent stem (iPS) cell technology, which allows adult cells to be "reprogrammed" to be stem cell-like cells. (ca.gov)
  • There are two types of stem cell manufacturing: embryonic stem cell production, derived from early-stage embryos, and induced pluripotent stem cell (iPSC) production, which reprograms adult cells to an embryonic-like state. (imarcgroup.com)
  • We have been using two complementary model systems, the genetically modified mouse system and patient-derived induced pluripotent stem cell (iPSC) model systems. (upenn.edu)
  • Mackay added that the iPSC-derived neural cells grown in culture "may not yield infection results that accurately reflect disease processes in humans. (the-scientist.com)
  • Comparative analysis of human induced pluripotent stem cell-derived mesenchymal stem cells and umbilical cord mesenchymal stem cells. (reprocell.com)
  • Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)
  • Strategies for therapeutic cell transplantation have been assessed for use in the treatment of massive peripheral nerve defects. (elsevierpure.com)
  • To support safe and efficient cell transplantation, we have focused on the purification of cells using cell surface markers. (elsevierpure.com)
  • In this study, we show that transplanted human fibroblasts and human astrocytes, which are engineered to express inducible forms of neural reprogramming genes, convert into neurons when reprogramming genes are activated after transplantation. (lu.se)
  • We monitor our cells after transplantation using standard in vivo assessments as listed above. (lu.se)
  • The clinical team from Lund headed by Prof. Olle Lindval in collaboration with Prof. Anders Björklund, were the first to show in patients the proof of principle that cell transplantation therapy is feasible approach to treat the patients with Parkinson's disease. (lu.se)
  • Current efforts in the Lund PD transplantation program are focused on the use of dopamine neurons derived from pluripotent stem cells, using a protocol that has been devloped here in Lund ( publications 9 below ). (lu.se)
  • With a $50,000 grant from FRAXA Research Foundation, Dr. Clive Svendsen and his team at the University of Wisconsin grew neural stem cells that expressed the Fragile X mutation to help scientists better understand the gene characteristics. (fraxa.org)
  • Scientists hope to gain valuable insights from the study of stem cells, e.g. in developmental biology. (eppendorf.com)
  • The induced pluripotent stem cell breakthrough continues to be useful in the very ways that "the scientists" once said only human cloning could provide. (cbc-network.org)
  • The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
  • VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)
  • B-D) NPCs maintained in STEMdiff™ Neural Progenitor Medium express the CNS-type NPC markers PAX6 (B, D, red), SOX1 (C, red) and NESTIN (C, green), but not the neural crest marker SOX10 (D, green, single channel shown in inset). (stemcell.com)
  • To this end, this article briefly discusses numerous important processes in head and neck embryology, namely the implications of patterning in hindbrain development, the diverse roles of neural crest cells, migration of the neural crest cells into the branchial arches (particularly the hyoid arch), and the genetic control of these processes. (medscape.com)
  • The development of the hindbrain (rhombencephalon) and the subsequent delamination of the neural crest cells are interrelated processes that need to be understood to appreciate the development of the branchiomotor cranial nerves in general and the facial nerve in particular. (medscape.com)
  • One example of constitutive euchromatin that is 'always turned on' is housekeeping genes, which code for the proteins needed for basic functions of cell survival. (wikipedia.org)
  • Intriguingly, the new study suggests the presence or absence of the genes used to reprogram skin cells to become the blank slate pluripotent cells makes no difference in terms of their capacity to differentiate. (scienceblog.com)
  • Some of the induced stem cell lines tested in the study were made using techniques that bypassed the use of genes that had been used to reprogram skin cells to become pluripotent stem cells. (scienceblog.com)
  • The standard tests for LQTS use animal models or hamster cells that express human heart genes at high levels. (ca.gov)
  • To facilitate this type of research in the autism field, SFARI has supported the generation of numerous iPS cell lines from individuals with genetic variants in high-confidence autism risk genes, who enrolled in the Simons Simplex Collection (SSC) and Simons Searchlight studies. (sfari.org)
  • Cellular reprogramming is a new and rapidly emerging field in which somatic cells can be turned into pluripotent stem cells or other somatic cell types simply by the expression of specific combinations of genes. (lu.se)
  • Using a transgenic mouse model to specifically direct expression of reprogramming genes to parenchymal astrocytes residing in the striatum, we also show that endogenous mouse astrocytes can be directly converted into neural nuclei (NeuN)-expressing neurons in situ. (lu.se)
  • article{4b5db5d0-c1b6-463c-9ca2-f61b01429744, abstract = {{Cellular reprogramming is a new and rapidly emerging field in which somatic cells can be turned into pluripotent stem cells or other somatic cell types simply by the expression of specific combinations of genes. (lu.se)
  • The rostral division of the neural tube into its 3 main sections falls under the control of homeobox (Hox) family of genes. (medscape.com)
  • Induced pluripotent stem cells for neural tissue engineering. (ca.gov)
  • By examining the process of stem cells turning into cells in bones, heart muscle, nerves, and other organs and tissue, researchers can better understand how diseases and conditions develop. (globenewswire.com)
  • New areas of study include the effectiveness of using human stem cells that have been programmed into tissue-specific cells to test new drugs. (globenewswire.com)
  • Cell and tissue banking applications will account for the leading share of the market by 2020. (globenewswire.com)
  • We developed a robust characterization methodology composed of quantitative bright-field absorbance microscopy (QBAM) and deep neural networks (DNNs) to noninvasively predict tissue function and cellular donor identity. (jci.org)
  • DNN predictions were supplemented with traditional machine-learning algorithms that identified shape and texture features of single cells that were used to predict tissue function and iPSC donor identity. (jci.org)
  • Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
  • A subset of NSCs is present in the developed nervous system and acts as a reservoir of cells for cell replacement and nervous tissue regeneration (PMID: 20110496). (sanbio.nl)
  • IPS cells derived from individuals with neurodevelopmental conditions provide a unique tool to examine early developmental changes in molecular and cellular mechanisms within the human genetic context, thus complementing studies that use human postmortem brain tissue and animal models. (sfari.org)
  • Discussion: Activation of the PI3K/AKT pathway decreases the concentration of apoptotic factors, while the HIF pathway protects stem cells against the micro-environment of tissue (hypoxia). (benthamscience.com)
  • With the right mix of nutrients and a little bit of coaxing, human stem cells derived from skin can assemble spontaneously into brain-like chunks of tissue. (cbc-network.org)
  • The researchers found that tissue chunks cultured from stem cells derived from the skin of a single human with microcephaly did not grow as big as clumps grown from stem cells derived from a healthy person. (cbc-network.org)
  • In this project, involving engineers from LTH, we apply advanced microfluidic techniques to culture hESCs under the influence of chemical gradients to mimic the environment around the developing brain in the fetus, thereby generating neural tissue with anatomical characteristics resembling the developing human brain. (lu.se)
  • Tissue-specific stem cells (also known as Somatic Stem Cells) that appear during fetal development and remain in the body throughout life. (bvsalud.org)
  • STEMdiff™ Neural Progenitor Medium is a defined and serum-free medium for the expansion of neural progenitor cells (NPCs) derived from human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium (Catalog #05835). (stemcell.com)
  • NPCs are distinct from differentiated cells of the nervous system at the molecular level. (sanbio.nl)
  • Immunofluorescent staining of PAX6 ( 12323-1-AP , 1:250 dilution) with 4% PFA fixed control human-induced pluripotent stem cell (hiPSC)-derived NPCs. (sanbio.nl)
  • NPCs can additionally be obtained by direct reprogramming of somatic cells from different cell lineage (PMID: 22445518). (sanbio.nl)
  • Alternatively, NPCs can be isolated from fresh nervous tissues using cell sorting with NPC markers (PMID: 11121071). (sanbio.nl)
  • We next demonstrate that co-culturing human NPCs with rodent astrocytes results in mutually synergistic maturation, and that cell type-specific expression data can be extracted using only sequencing read alignments without cell sorting. (bvsalud.org)
  • Here, we show that the protein level of ClpP is selectively decreased in αSyn-expressing cell culture and neurons derived from iPS cells of PD patient carrying αSyn A53T mutant, and in dopaminergic (DA) neurons of αSyn A53T mice and PD patient postmortem brains. (springer.com)
  • That means that at this point there is still some work to be done to generate ideal induced pluripotent stem cells for application. (scienceblog.com)
  • Using Microfluidics to Generate Human Naïve and Primed Pluripotent Stem Cells. (reprocell.com)
  • NSCs are derived from the embryonic stem (ES) cells or fetal and adult nervous system, and can generate different type of neural cells. (lu.se)
  • Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)
  • By viral expression of neural fate determinants, it is possible to directly reprogram mouse and human fibroblasts into functional neurons, also known as induced neurons. (lu.se)
  • Secondly, we have developed an all-in-one vector-based strategy to convert adult human dermal fibroblasts directly from Huntington's disease (HD) patients and control individuals into induced neurons (iNs). (lu.se)
  • One way we did this was by converting adult fibroblasts, or skin cells, into interneurons. (lu.se)
  • This driver is fueled by the promising prospects of stem cell therapies in addressing previously untreatable conditions, such as neurodegenerative diseases and spinal cord injuries. (imarcgroup.com)
  • AD is a severe progressive neurodegenerative disease, which induces decreasing memory capacity and cognition. (bvsalud.org)
  • Andreas' research focuses on generating brain cells, specifically interneurons, in the lab, which has significant implications for the study of neurodegenerative and neuropsychiatric disorders. (lu.se)
  • The main objective of the Stem Cell Center´s Neuroscience Program is to study the properties of neural stem cells (NSCs) and explore the possibilities to use these cells for treatment of neurodegenerative diseases such as Parkinson's disease and stroke. (lu.se)
  • RESULTS: In addition to modules that govern loss of pluripotency and gain of neural ectoderm identity, we discover other modules governing cell cycle and metabolism. (ox.ac.uk)
  • During the first 4 weeks of embryogenesis, the notochord induces axial ectoderm to form the neural plate, which then folds along its long axis to form the neural tube. (medscape.com)
  • Rising stem cell research and increasing demand for stem cell therapies are driving the growth of this segment. (globenewswire.com)
  • Increasing investments in stem cell research, rising chronic disease prevalence, advancements in biotechnology, collaborations between academia and industry, stringent regulations, and expanding awareness of stem cell therapies are stimulating the market growth. (imarcgroup.com)
  • Apart from this, the increasing awareness and acceptance of stem cell-based therapies among patients and healthcare professionals is augmenting the market growth. (imarcgroup.com)
  • Stem cell therapies hold immense promise in addressing these ailments by regenerating damaged tissues and organs. (imarcgroup.com)
  • Increases in the number of cell therapies in the preclinical and clinical phases have prompted the need for reliable and noninvasive assays to validate transplant function in clinical biomanufacturing. (jci.org)
  • Our main focus is to enhance nerve regeneration following spinal cord injury via two strategies: (1) cell therapies within the injured spinal cord and (2) gene therapies within the motor cortex in the brain. (ccf.org)
  • Zhang's group, led by researcher Baoyang Hu, found that the induced cells differentiate into progenitor neural cells and further into the different kinds of functional neurons that make up the brain. (scienceblog.com)
  • Even though functional changes are clearly expressed in similar manner in relation to distinct neurodevelopmental disorders, in human neural progenitor cultures there are a lot of individual variations that presumably regulate the effects of each gene mutation on the individual phenotype," Castrén elaborates. (neurosciencenews.com)
  • Using the temporal relationships between the changing profile of key transcription factors and subsequent changes in their target gene expression profiles, we have identified distinct functional modules operative throughout neural induction. (ox.ac.uk)
  • Strikingly, some of these functional modules are retained throughout neural induction, even though the gene membership of the module changes. (ox.ac.uk)
  • Single transcription factor efficiently leads human induced pluripotent stem cells to functional microglia. (reprocell.com)
  • This includes long-term survival of transplanted cells in vivo to assess cell fate, maturation and integration, as well as functional behavioural assessment for graft function in vivo. (lu.se)
  • To reduce this heterogeneity, we generated engineered cerebral organoids (enCORs), which use a floating scaffold to increase the efficiency of neural induction and reduce heterogeneity. (researchsquare.com)
  • Temporal transcriptional control of neural induction in human induced pluripotent stem cells. (ox.ac.uk)
  • INTRODUCTION: Neural induction of human induced pluripotent stem cells represents a critical switch in cell state during which pluripotency is lost and commitment to a neural lineage is initiated. (ox.ac.uk)
  • Although many of the key transcription factors involved in neural induction are known, we know little of the temporal and causal relationships that are required for this state transition. (ox.ac.uk)
  • We then focussed on OTX2, one of the most precociously activated transcription factors during neural induction. (ox.ac.uk)
  • Further CRISPRi inhibition of OTX2 prior to neural induction promotes an accelerated loss of pluripotency and a precocious and aberrant neural induction disrupting some of the previously identified modules. (ox.ac.uk)
  • DISCUSSION: We infer that OTX2 has a diverse role during neural induction and regulates many of the biological processes that are required for loss of pluripotency and gain of neural identity. (ox.ac.uk)
  • Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
  • Progressing a cell-based therapeutic from pre-clinical stage production through later clinical stages and into commercial production requires scale-up, which is one of the chief drivers for the stem cell manufacturing market growth. (globenewswire.com)
  • The advantages of stem cell manufacturing are manifold, as it offers a renewable source of cells for research, drug testing, and potential therapeutic applications. (imarcgroup.com)
  • Additionally, innovations in genetic editing techniques, like CRISPR-Cas9, offer precise control over stem cell characteristics, further expanding their therapeutic potential. (imarcgroup.com)
  • However, the combined effect of prophylactic zinc administration and therapeutic taurine treatment on intrauterine ischemia- (IUI-) induced cerebral damage remains unknown. (hindawi.com)
  • The therapeutic candidate comprises of human autologous induced pluripotent (iPS) derived neural stem cells (NSC). (pharmaceutical-technology.com)
  • This high throughput drug screen uses neural stem cells from Fragile X knockout mice to identify small molecules which may be therapeutic in Fragile X. (fraxa.org)
  • He received his PhD from the University of California Department of Biology and went on to complete his postdoctoral fellowship at the University of California San Diego (UCSD) in the Center for Neural Repair, under the guise of Professor Mark Tuszynski, MD, PhD. Poplawski has also published in high impact journals such as Nature , Science Translational Medicine , and Neuron . (ccf.org)
  • Development: For advances in developmental biology and stem cells. (lu.se)
  • Combining cell biology and electrophysiology, his work has the potential to create personalized disease models for future research. (lu.se)
  • Lund center for Stem Cell Biology and Cell Therapy is one of six Swedish strategic centers of excellence in life sciences, supported by the Swedish Foundation for Strategic Research. (lu.se)
  • Her research focuses on microglia, the immune cells of the brain, where she has developed protocols to immunophenotype microglia in humans using markers relating to a specific function allowing us to investigate the role of these cells in different neurological conditions. (southampton.ac.uk)
  • Using super-resolution structured illumination microscopy, we have explored the mechanism by which nucleoporins are altered in nuclei isolated from C9orf72 induced pluripotent stem-cell-derived neurons (iPSNs). (nih.gov)
  • By identifying the critical mechanisms for stroke and neural recovery, we are able to develop polymeric technologies for clinical translation in nerve regeneration and stroke recovery. (stanford.edu)
  • Bach, E.A. JAK/STAT signaling in stem cells and regeneration: From drosophila to vertebrates. (benthamscience.com)
  • Regeneration of Corticospinal Axons into Neural Progenitor Cell Grafts After Spinal Cord Injury. (ccf.org)
  • These iPS cells could lead to cures for various disorders and to organ regrowth and regeneration. (answersingenesis.org)
  • They transplanted human Fragile X neural cells differentiated from induced pluripotent stem cells into brains of neonatal mice and then testing for FMR1 reactivation. (fraxa.org)
  • Prof. Lindvall and Dr. Kokaia were the first in the world to show that the brain produces new neurons in response to stroke which opened new possibilities for cell therapy by developing the way to modulate and direct brains this self-repair mechanisms. (lu.se)
  • Drug-induced LQTS is the single most common reason for drugs to be withdrawn from clinical trials, causing major setbacks to drug discovery efforts and exposing people to dangerous drugs. (ca.gov)
  • In most cases, the mechanism of drug-induced LQTS is unknown. (ca.gov)
  • Despite the critical need, current tests for drug-induced LQTS are far from perfect. (ca.gov)
  • These studies will provide the foundation for an expanded panel of iPS cell lines from people with other genetic mutations and from people who have no genetically defined risk factor but still have potentially fatal drug-induced LQTS. (ca.gov)
  • The Simons Foundation Autism Research Initiative (SFARI) hosted a virtual workshop on March 14 and 18, 2022, to discuss the best practices for the development and use of induced pluripotent stem (iPS) cell models for autism research. (sfari.org)
  • Zika virus directly infects peripheral neurons and induces cell death. (upenn.edu)
  • AChE inhibition induces an acetylcholine build-up, disrupting signals between nerves and muscles, which in high doses leads to asphyxiation. (cdc.gov)
  • But, he adds, the study also demonstrates the potential for using human-stem-cell-derived tissues to model other disorders, if cell growth can be controlled more reliably. (cbc-network.org)
  • The key functions of adult stem cells are to maintain and repair the specific tissues where they reside (e.g. skin or blood). (bvsalud.org)
  • We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)
  • The George lab develops biomaterials to improve neural recovery in the peripheral and central nervous systems. (stanford.edu)
  • Major factors driving market revenue growth include increasing use of stem cells to treat various diseases, rising stem cell-based research activities, and growing funding from private and public organizations for stem cell-based research. (globenewswire.com)
  • Stem cell manufacturing involves production of stem cells that can be used in cell-based research and therapeutics development. (globenewswire.com)
  • With a $90,000 grant from FRAXA Research Foundation, Dr. Peng Jin's team from Emory University School of Medicine found that Fragile X causes an increase production of new cells, so they tested large numbers of drugs to find those that can correct this. (fraxa.org)
  • Recent work studies the effect of electrical stimulation on neural stem cells seeded on the conductive scaffold and the pathways by which it enhances stroke recovery Further understanding the combined effect of electrical stimulation and stem cells in augmenting neural repair for clinical translational is a major focus of this research going forward. (stanford.edu)
  • Methods: This article focuses on recent research development that addresses the viability issues of stem cells. (benthamscience.com)
  • This new research simply emphasizes the fact that adult stem cells are worth their weight (pun intended). (answersingenesis.org)
  • Research groups are gathered within five main research clusters: MultiPark, Stem Cell Center and Epilepsy Center. (lu.se)
  • The possibility to isolate and propagate NSCs and their potential applications in cell therapy have attracted a lot of research interest in recent years. (lu.se)
  • The finding that induced cells are less predictable means there are more kinks to work out before they can be used reliably in a clinical setting, says Su-Chun Zhang, the senior author of the new study and a professor in the University of Wisconsin-Madison School of Medicine and Public Health. (scienceblog.com)
  • Such unknowns would also limit their use in clinical settings for such things as cell transplants. (scienceblog.com)
  • Neuron Stem Cells (iNSC) is under clinical development by Allife Medical Science and Technology and currently in Phase II for Parkinson's Disease. (pharmaceutical-technology.com)
  • The methodology was validated using clinical-grade induced pluripotent stem cell-derived retinal pigment epithelial cells (iPSC-RPE). (jci.org)
  • non-embryonic stem cells have had proven laboratory and clinical successes and don't require any loss of human life. (answersingenesis.org)
  • However, a new study published this week (Feb. 15) in the Proceedings of the National Academy of Sciences comparing the ability of induced cells and embryonic cells to morph into the cells of the brain has found that induced cells - even those free of the genetic factors used to program their all-purpose qualities - differentiate less efficiently and faithfully than their embryonic counterparts. (scienceblog.com)
  • The new Wisconsin study compared the ability of five embryonic stem cell lines with 12 induced cell lines coaxed into being using different methods. (scienceblog.com)
  • A case-control and seven-year longitudinal neurocognitive study of adults with sickle cell disease in Ghana. (ucl.ac.uk)
  • T Cell Responses to Dystrophin in a Natural History Study of Duchenne Muscular Dystrophy. (ucl.ac.uk)
  • The study demonstrates that human neuron-like cells can be infected with Zika virus and that infection leads to death and reduced growth of the infected cells," said microbiologist and immunologist Andrew Pekosz of Johns Hopkins University who was not involved in the study. (the-scientist.com)
  • This is important because this may be a way to study the damage induced directly by infection. (the-scientist.com)
  • Now, in what appears to be a very notable breakthrough, they have been used to make patient-specific, tailor made neural cells for study. (cbc-network.org)
  • Dr Evelyn Heck from Argentina will join Prof Francisco Quintana in Boston, USA, at the Brigham and Women's Hospital, to study the molecular mechanisms of the relationship between Epstein-Barr Virus persistent in B cells and the development of MS. (msif.org)
  • This study provided important proof-of-principle that direct neural conversion of endogenous glia can take place in the adult rodent brain. (lu.se)
  • Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
  • Cell Therapy for Parkinson's Disease: What Next? (lu.se)
  • iPS cells can be grown and differentiated into many different brain cell types, as well as 3D brain organoids 1,2 . (sfari.org)
  • Cell Stem Cell 28: 1657-1670, Sep 2021. (upenn.edu)
  • Results: To increase the viability of stem cells, techniques like scaffolds of stem cells with hydrogel or alginate, pre-conditioning, different routes of administration and encapsulation, are implemented. (benthamscience.com)
  • Embryonic stem cells can pretty much be predicted," says Zhang. (scienceblog.com)
  • It was predicted, Zhang explains, that the absence of exotic genetic factors would result in cells essentially identical to embryonic stem cells. (scienceblog.com)
  • Despite their unpredictability, Zhang notes that induced stem cells can still be used to make pure populations of specific types of cells, making them useful for some applications such as testing potential new drugs for efficacy and toxicity. (scienceblog.com)
  • Zhang, Y. Integrin β1 increases stem cell survival and cardiac function after myocardial infarction. (benthamscience.com)