Neural gene transfer techniques. Medical search. Frequent questions

These methods today are routinely used for gain-of-function analysis, to overexpress or ectopically express genes of interest (Arber et al. (ox.ac.uk)
The development of transgenic mouse models that express genes of interest in specific cell types has transformed our understanding of basic biology and disease. (nature.com)

assays of chromatin and chromatin structure in neurons, and mRNA isolation from specified neural subtypes by TRAP. (cshl.edu)
Dr. Sieck's team has also developed a novel targeted gene therapy approach to increase TrkB expression in phrenic motor neurons using an adeno-associated virus. (mayo.edu)
Although electroporation and viral vector-mediated gene delivery techniques have been used to introduce exogenous DNA into chicken embryonic cells, transducing neurons efficiently and specifically remains challenging. (biomedcentral.com)
On the other hand, optogenetic control of neuronal activity allows us to selectively activate or inactivate genetically defined populations of neurons in order to examine how the activity of these neurons contributes to the function of neural circuits in the brain. (jnss.org)
In both models, we found unchanged cell proliferation of the neural precursors in the DG subgranular zone, whereas a significant increase in the survival of newborn neurons was noted 1 and 4weeks after BrdU injections. (atpase-receptor.com)
Optogenetic approaches promise to revolutionize neuroscience by using light to manipulate neural activity in genetically or functionally defined neurons with millisecond precision. (nature.com)
These techniques provide a precision in the temporal and spatial activation of neurons that was not achievable in previous experiments. (nature.com)
The study has found that when a suspected autism gene called PTEN is deleted from auditory cortical neurons-the main workhorses of the brain's sound-processing center-the signals that these neurons receive from local as well as long-distance sources are strengthened beyond normal levels. (neurosciencenews.com)
To decipher the role of PTEN on functional connectivity in the auditory cortex, Zador's group selectively disrupted the function of the PTEN gene in adult mice, only in a subset of neurons of the auditory cortex, while leaving the gene intact in neighboring neurons. (neurosciencenews.com)

The expression of the target gene is regulated only in the cells with double transfection by the two vectors. (news-medical.net)
We are investigating how female lymphocytes maintain X-chromosome Inactivation, which is an epigenetic process responsible for equalizing gene expression between sexes. (upenn.edu)
For short-term experiments, electroporation and adenoviral-mediated gene transfer methods provide transient expression, largely because of the short persistence time of the transgene within the cell. (ox.ac.uk)
In the present study, we performed a comparative study of the ubiquitous CMV promoter and three neuron-specific promoters, chicken Ca2+/calmodulin-dependent kinase (cCaMKII), chicken Nestin (cNestin), and human synapsin I. We explored the possibility of manipulating gene expression in chicken embryonic brain cells using in ovo electroporation with the selected promoters. (biomedcentral.com)
Overexpression of the FOXP2 gene driven by the cNestin promoter in brain cells significantly affected expression levels of target genes, CNTNAP2 and ELAVL4 . (biomedcentral.com)
In ovo electroporation offers an easier and more efficient way to manipulate gene expression during embryonic development, and this technique will be useful for neuron-targeted transgene expression. (biomedcentral.com)
This research often requires the tissue-specific expression of transgenes to better mirror human diseases, to visualize pathways being studied or to modulate the expression of a disease-related gene. (nature.com)
Transgene expression using viral vectors provides an alternative, which allows for temporal expression of genes in mice with reduced cost and effort. (nature.com)
LN-T cells derived from donor trained vs. sedentary PLP-immunized mice were analyzed in vitro for proliferation assays and cytokine and chemokine receptor genes expression. (pulsus.com)
Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
The study of human papillomavirus (HPV)-induced carcinogenesis uses multiple in vivo mouse models, one of which relies on the cytokeratin 14 gene promoter to drive the expression of all HPV early oncogenes. (bvsalud.org)
Comparing differential gene expression can help to determine the effect of radiation and estrogens on cell adhesion. (bvsalud.org)
Results indicated that most genes had greater expression in the tumorigenic cell line Tumor2 derived from the athymic animal than the Alpha3, a non-tumorigenic cell line exposed only to radiation, indicating that altered expression levels of adhesion molecules depended on estrogen. (bvsalud.org)

Epithelial to mesenchymal transition during mammalian neural crest cell delamination. (stowers.org)
Human embryonic stem cells have been coaxed into becoming neural crest stem cells, an important cell type in the developing embryo. (hum-molgen.org)
consequently, melanomas, although they usually occur on the skin (see the image below), can arise in other locations where neural crest cells migrate, such as the gastrointestinal tract and brain. (medscape.com)
These cells are derived from the neural crest. (medscape.com)

Single-cell transcriptomic signatures and gene regulatory networks modulated by W1s in mammalian midline facial formation and clefts. (stowers.org)

With the already impressively successful application of light-driven actuator proteins such as microbial opsins to interact with intact neural circuits, optogenetics rose to a key technology over the past few years. (laserglow.com)

Chick in ovo electroporation is a technique which allows genetic manipulation of the avian embryo. (jove.com)
This video demonstrates neural tube electroporation in HH 10 chick embryos. (jove.com)

Neural Networks, 2022, 145: 209-220. (xjtlu.edu.cn)
RamGAN: Region Attentive Morphing GAN for Region-Level Makeup Transfer", European Conference on Computer Vision (ECCV), 2022. (xjtlu.edu.cn)
Krishnagopal S, Lohse KR, Braun, RG* (2022) Stroke recovery phenotyping through network trajectory approaches and graph neural networks . (umaryland.edu)

The chicken in ovo model is an attractive system to explore underlying mechanisms of neural and brain development, and it is important to develop effective genetic modification techniques that permit analyses of gene functions in vivo. (biomedcentral.com)
The enhanced efficacy and exquisite specificity of SELECTIV has broad utility in development of new mouse model systems and expands the use of AAV for gene delivery in vivo. (nature.com)

Optogenetics provides a means of switching neural circuits on and off, at high speed and with little cross-talk. (laserglow.com)
The neural network within the auditory cortex has therefore been a target of studies aimed at understanding how alterations in neural circuits contribute to dysfunction in ASDs. (neurosciencenews.com)
People who drink to forget bad memories may actually be doing the opposite by reinforcing the neural circuits that control negative emotional memory," he continued. (nootropic.com)

Scientists anticipate that in the future stem cell lines will provide a virtually unending supply of pancreatic cells for diabetic patients, neuronal cells for patients with neural disorders such as Parkinson's or Alzheimer's disease, and a host of heart cells that may treat a variety of cardiac problems. (spiked-online.com)
Alternatively, transgenesis and gene targeting techniques can be used to introduce the patient's genes into the stem cell line. (spiked-online.com)
Here, the stem cell line is created using the genetic properties of the prospective recipient via somatic cell nuclear transfer. (spiked-online.com)
Blastocytes obtained through nuclear transfers would be used to generate the embryonic stem cells that could be differentiated to specific tissues or organs for transfer to the nuclear donor. (spiked-online.com)
Prof. Einstein's area of specialization is neuro-immunology and neuro-regeneration in neurodegenerative diseases, specifically on animal models of human Multiple Sclerosis (MS). Her major studies concern on the neurobiology of neural stem cells and cell therapy in neurodegenerative diseases. (pulsus.com)
Her work published in 2003 was the first to show that transplanted neural stem cells have antiinflammatory effects on the rodent brain. (pulsus.com)
A method of reprogramming rhesus macaque adult fibroblasts into embryonic stem cells using somatic cell nuclear transfer is presented. (hum-molgen.org)
While in this program, I collaborated with the Childers and Mack labs at the Institute for Stem Cell and Regenerative Medicine and initiated a new line of research examining the effects exercise on gene therapy uptake for inherited myopathies. (umaryland.edu)

It's exciting to find something you can use to manipulate neural growth that's easy and noninvasive like controlling the oxygen environment," she said. (neurosciencenews.com)

However, in the last decade and a half, a number of new methods for transient transgenesis have been developed that allow efficient alteration of gene function during early embryonic development. (ox.ac.uk)
While attempts to establish germ line transgenesis are ongoing, methods for rapid, transient spatiotemporally targeted gene alterations have thus again re-established the chick embryo as an important experimental niche by making it possible to apply genetics in concert with classical embryological techniques. (ox.ac.uk)
Two common approaches for transferring this behavior are germline transgenesis and somatic gene delivery . (laserglow.com)

This report describes the role of neuroprotection in acute disorders such as stroke and injuries of the nervous system as well as in chronic diseases such as neurodegenerative disorders because many of the underlying mechanisms of damage to neural tissues are similar in all these conditions and several products are used in more than one disorder. (researchandmarkets.com)

Dr. Sieck has developed an extensive array of state-of-the-art physiological, molecular and biomedical engineering techniques to explore cell signaling pathways. (mayo.edu)
The construction of gene regulatory networks (GRNs) is vital for understanding the regulation of metabolic pathways, biological processes, and complex traits during plant growth and responses to environmental cues and stresses. (mtu.edu)
The challenge therefore has been to understand how this diverse set of candidate genes and the pathways they control converge to cause the common signature of ASDs," Zador says. (neurosciencenews.com)

New research from Cold Spring Harbor Laboratory (CSHL) might help explain how a gene mutation found in some autistic individuals leads to difficulties in processing auditory cues and paying spatial attention to sound. (neurosciencenews.com)
StyleGene: Crossover and Mutation of Region-Level Facial Genes for Kinship Face Synthesis. (xjtlu.edu.cn)

The collaborative research team led by Professor Tadashi ISA, Project Assistant Professor Masaharu KINOSHITA from The National Institute for Physiological Sciences, The National Institutes of Natural Sciences and Fukushima Medical University and Kyoto University, developed 'the double viral vector transfection technique' which can deliver genes to a specific neural circuit by combining two new kinds of gene transfer vectors. (news-medical.net)
Professor Tadashi ISA says 'this newly developed double viral vector transfection technique can be applied to the gene therapy of the human central nervous system, as we are the same higher primates. (news-medical.net)
Alternative use of animal oocytes will need to be considered and the relative risk of human to animal viral transfer tackled (3). (spiked-online.com)

These new technologies include 'optogenetics' - a growing suite of techniques that combine optical and molecular genetic methods. (jnss.org)
Fundamental questions that neuroscientists have previously approached with classical biochemical and electrophysiological techniques can now be addressed using optogenetics. (laserglow.com)
The term optogenetics reflects the key program of this emerging field, namely, combining optical and genetic techniques. (laserglow.com)

Because these sensors can be introduced by gene transfer techniques, they may extract neuronal signals from an intact brain more efficiently than conventional organic dyes. (jnss.org)
The scientists then assessed the effect of the loss of PTEN on connectivity within the auditory cortex using techniques that involve stimulation by laser or flashes of blue light to trigger neuronal activity either locally or in other brain areas that send neuronal projections into the auditory cortex. (neurosciencenews.com)
Doctors already used a similar technique in a study of patients with chronic, incomplete spinal cord injury - patients whose spinal cords are injured but in such a way that some communication between the brain and spinal cord exists, allowing limited feeling and movement. (neurosciencenews.com)
During the final year of medical school and first year of residency as Intern in Internal Medicine at Loyola, I published several manuscripts (in Brain and Cognition, Neurorehabilitation and Neural Repair, and Somatosensory and Motor Research), and was awarded the 2010 ERF New Investigator Award by the Foundation for PM&R. (umaryland.edu)
Multi-Classification of Brain Tumors on Magnetic Resonance Images Using an Ensemble of Pre-Trained Convolutional Neural Networks. (cdc.gov)

For instance, the technique can be applied to channelrhodopsin cation channels to initiate neuronal depolarization and eventually activation upon illumination. (wikipedia.org)

This phase is characterized by the development of methods for biological design of plant varieties, including transformation and gene editing techniques directed toward causal loci. (springer.com)

Overall, our results indicate that constitutive 5-HT depletion does not alter the proliferation of neural precursors in the DG but promotes the survival of newborn cells, an effect which involves activation of postsynaptic 5-HT1A receptors. (atpase-receptor.com)
Well known for its role as an anti-cancer gene that powers down cell growth, proliferation and survival, this gene has also been linked to ASDs by a slew of studies in humans and mice. (neurosciencenews.com)

Maternal serum screening for neural tube defects Noninvasive prenatal screening for genetic disorders, unlike invasive testing, has no risk of test-related complications. (msdmanuals.com)

Employing transfer learning techniques, this study has also successfully transferred the known knowledge of gene regulation from one species to another, substantially improving performance and manifesting the viability of cross-species learning using deep learning-based approaches. (mtu.edu)
A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Because these approaches will rely on novel data types such as DNA sequences and high-throughput phenotyping images, Breeding 4 will call for analyses that are complementary to traditional quantitative genetic studies, being based on machine learning techniques which make efficient use of sequence and image data. (springer.com)
Looking to the future, the pairing of integrative taxonomy approaches with multi-locus genomic sequencing and phenomic techniques, such as transcriptomics and proteomics, holds great potential to resolve many unknowns in fungal taxonomic classification. (frontiersin.org)

Proteolipid protein (PLP) -induced transfer EAE was utilized to examine ET effects specifically on the systemic immune system. (pulsus.com)
This model was used to determine genes associated with cell adhesion and communication such as E-cadherin, the desmocollin 3, the gap junction protein alpha 1, the Integrin alpha 6, the Integrin beta 6, the Keratin 14, Keratin 16, Keratin 17, Keratin 6B, and the laminin beta 3. (bvsalud.org)

These techniques have made it possible to study the effects of gene inactivation or overexpression on downstream transcriptional regulation as well as on embryonic derivatives. (ox.ac.uk)

To examine effects of ET on systemic autoimmunity, lymph-node (LN)-T cells from trained- vs. sedentary donor mice were transferred to naive recipients and EAE severity was assessed. (pulsus.com)
Mice that lack a particular gene die suddenly and without overt signs of illness in response to an infection that is usually harmless. (hum-molgen.org)
A gene that allows mice to accept human bone marrow cells more efficiently is presented online. (hum-molgen.org)

Using a variety of cell culture systems, molecular virology techniques, and patient-derived samples, I investigate the pathogenic and evolutionary mechanisms by which viruses interact with the host and cause disease. (uc.edu)
Using cortical connectivity as a paradigm for assessing ASD candidate genes could provide insights into the mechanisms of the disorders and perhaps even give us clues to formulate new therapeutic strategies," he states. (neurosciencenews.com)

Using this technique, they succeeded in the suppression of the propriospinal neuron selectively and reversibly. (news-medical.net)

Bayesian neural networks (BNNs) promise improved generalization under covariate shift by providing principled probabilistic representations of epistemic uncertainty. (icml.cc)
Neural Networks 136 (2021): 233-243. (xjtlu.edu.cn)
These models are often run on neural networks and may learn to detect the data's inherent distinguishing qualities. (washingtonindependent.com)
Neural networks then use these simplified core understandings of real-world data to model data that looks like or is the same as real-world data. (washingtonindependent.com)
Deep Neural Networks and Machine Learning Radiomics Modelling for Prediction of Relapse in Mantle Cell Lymphoma. (cdc.gov)

Well-known scientific advancements such as the use of electrical stimuli and electrodes have succeeded in neural activation but fail to achieve the aforementioned goal because of their imprecision and inability to distinguish between different cell types. (wikipedia.org)
A renewable, tissue culture source of human cells capable of differentiating into a wide variety of cell types would have broad applications in basic research and therapeutic techniques. (spiked-online.com)
Human nuclear cell transfer is legal in the UK, but is liable to become a criminal act in the USA pending an upcoming vote in the Senate. (spiked-online.com)
Her current research focuses mainly on neuro-immunological, neuro-protective and neuro-regenerative effects of exercise training on neurodegenerative diseases, particularly on experimental autoimmune encephalomyelitis (EAE) the animal model of MS. The studies involve animal training, clinical evaluations, histopathological analyses, cell cultures and molecular biology techniques. (pulsus.com)
Each correctly classified pharmaceutical text is further analyzed by recognizing combined entities through the proposed models, as well as by using BioBERT for the detection of BC5CDR 4 and BioNLP13CG 5 tags [36], which include Disease, Chemical, Cell, Organ, Organism, Gene, etc. (researchgate.net)
If the oxygen level is normal, there isn't much H1F-1alpha, but in stress conditions such as a lack of oxygen, there is more of this gene to protect the cell and organism from this stress. (neurosciencenews.com)
Over 500 products have been investigated for neuroprotective effects including those from the categories of free radical scavengers, anti-excitotoxic agents, apoptosis (programmed cell death) inhibitors, anti-inflammatory agents, neurotrophic factors, metal ion chelators, ion channel modulators, and gene therapy. (researchandmarkets.com)
An in vitro experimental breast cancer model was developed by exposure of the immortalized human breast epithelial cell line MCF-10F to low doses of high linear energy transfer α particle radiation and subsequent growth in the presence of 17ß-estradiol. (bvsalud.org)

For long-term analyses, retrovirally mediated gene transfer has particular advantage. (ox.ac.uk)

New basic knowledge may lead to a knowledge transfer to technological fields resulting from interactions of citations, and, in this way, promotes innovations in industry and progress in society ( Hu & Rousseau, 2018 ). (sciendo.com)

These domestication traits likely consisted of a few major genes controlling dramatic changes in crop physiology, as is suggested by genetic studies comparing maize to its ancestor teosinte (Doebley et al. (springer.com)
The prokaryote-derived CRISPR (clustered regularly interspaced short palindromic repeats) is a genetic engineering technique in molecular biology by which the genomes of living organisms, including humans, may be modified ( Ledford, 2015 ). (sciendo.com)

The overall goal of my research program is computational model and neural data driven development of neuromodulation paradigms for improving cognitive control in patients with neurological and psychiatric disorders. (uc.edu)

Individuals with a neurodegenerative disease called spinocerebellar ataxia 11 (SCA11) have mutations in a gene called TTBK2. (hum-molgen.org)

Besides, this incredible technique over our heritable information, CRISPR, also brings unknown risks to human beings. (sciendo.com)
In this article, we present a new perspective on observing and understanding the research topics and their development in CRISPR based on the LDA model and try to illustrate the knowledge transfer trends from research topics to technological fields based on paper-patent citations. (sciendo.com)

Nonetheless, our results demonstrate that hybrid models integrating ML and artificial neural network (ANN) techniques significantly outperformed traditional methods in predicting gene regulatory relationships. (mtu.edu)
This study contributes to the methodology for growing body of knowledge in GRN prediction and construction for plant species, highlighting the value of adopting hybrid models and transfer learning techniques. (mtu.edu)
The proposed methodology is used to produce reliably labeled datasets leveraging cutting-edge transfer learning, which are later used to train models for specific entity labeling tasks. (researchgate.net)
By building models for the well-known text-processing libraries spaCy and AllenNLP, this technique is used to find Pharmaceutical Organizations and Drugs in texts from the pharmaceutical domain. (researchgate.net)

Gene-environment interactions in the pathogenesis of common craniofacial anomalies. (stowers.org)
Most of the platforms are expected to be connected to resources for handling various types of user interactions, conceivably with a back-end database management system and a front-end web based technique to handle user interactions. (lu.se)

And this is the discovery which reverses the general idea that the spinal cord is only a reflex pathway, but also plays a pivotal role in integrating the complex neural signals which enable dexterous movements. (news-medical.net)

Challenges arise due to limited training data for gene regulatory pairs, especially in less-studied species such as poplar and maize. (mtu.edu)
Although ASDs could arise from mutations in any of dozens of candidate genes, a core triad of symptoms defines all cases: impaired language, impaired social interaction, and restricted and repetitive behaviors. (neurosciencenews.com)

Zador's team focused for several reasons on the role of one suspected autism candidate gene, PTEN, on circuit alterations within the auditory cortex. (neurosciencenews.com)

Development and External Validation of a Machine Learning Model for Prediction of Potential Transfer to the PICU. (cdc.gov)

Engineering and evolution of synthetic adeno-associated virus (AAV) gene therapy vectors via DNA family shuffling. (nature.com)

Recently, photoinhibition, the inhibition of neural activity with light, has become feasible with the application of molecules such as the light-activated chloride pump halorhodopsin to neural control. (wikipedia.org)
Motor units are the essential elements of neural control. (mayo.edu)

Glioma Tumors' Classification Using Deep-Neural-Network-Based Features with SVM Classifier. (cdc.gov)

The increasing availability of public databases has facilitated the development of numerous methods for inferring gene regulatory relationships between transcription factors and their targets. (mtu.edu)
A Robust Vehicle Detection Model for LiDAR Sensor Using Simulation Data and Transfer Learning Methods. (swansea.ac.uk)

Using this method, further development of gene therapy targeted to a specific neural circuit can be expected. (news-medical.net)

However, there is limited research on supervised learning techniques that utilize available regulatory relationships of plant species in public databases. (mtu.edu)

A paper reports use of newly available technology and huge numbers of patients to reveal two MHC genes that are implicated in the development of childhood diabetes. (hum-molgen.org)

Mutations in a gene expressed in smooth muscle cells account for about 14% of cases of hereditary thoracic aortic aneurysm. (hum-molgen.org)
Lastly, we evaluated the strategy of knocking out a-syn as a means to protect the cells from transfer of pathology upon grafting. (lu.se)

Anatomy6

Organisms4

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Psychiatry and Psychology1

Phenomena and Processes16

Information Science2

Health Care1

Express genes of interest2

Neurons9

Expression13

Crest4

Mammalian1

Proteins1

Electroporation2

20223

Vivo2

Circuits3

Stem8

Manipulate1

Transgenesis3

Tissues1

Pathways3

Mutation2

Viral3

Optogenetics3

Brain5

Neuronal1

Biological1

Proliferation2

Noninvasive1

Approaches4

Protein2

Regulation1

Mice3

Mechanisms2

Neuron1

Networks5

Cell8

Analyses1

20181

Genetic2

Disorders1

Neurodegenerative1

CRISPR2

Models4

Interactions2

Signals1

Arise2

Alterations1

Prediction1

Vectors1

Control2

Network1

Methods2

Therapy1

Limited research1

Newly1

Cells2