Stable, long-term expression of your Custom Gene for your cell line of interest with custom selection marker (Puromycin, Blasticidin or Neomycin). (virostem.com)
Antibiotic1
Description: Pre-made lentiviral particles expressing GFP with Neomycin antibiotic marker, concentrated particles provided in PBS solution. (gentaurtop.com)
This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. (microbiologystudents.com)
Promoter1
The plasmid (GAL4RE)5-betaGlob-Luc-SVNeo contains a luciferase gene driven by a pentamer of yeast activator GAL4 binding sites in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. (inserm-transfert.com)
Lentivirus1
Unless the cells used stably produce rtTA, it is necessary to cotransduce this lentivirus with a rtTA lentivirus ( rtTA (CMV, neomycin) Lentivirus or rtTA (CMV, puromycin) Lentivirus . (kerafast.com)
Streptomycin1
Within this plasmid approximately four thousand base pairs upstream of the IncN conjugative region, there is a multiresistance region encoding resistance to neomycin ( aph-(3')-I ) followed by genes that confer resistance to puromycin, streptomycin ( aada1 -like), spectinomycin ( aada1 -like), and trimethoprim ( dfr12 ). (confex.com)
Hygromycin1
Full-length canine NCX1 a kind gift from Dr. Kenneth Philipson UCLA (19) was transfected in β-KD cells using the calcium phosphate transfection method and NCX1 expressing β-KD cells were selected with 10 μg/ml puromycin and 100 μg/ml hygromycin post transfection. (researchatlanta.org)
Useful in CRISPR/Cas9 mammalian gene editing by selecting for successful Cas9-induced knock-in with Puromycin resistance gene. (canvaxbiotech.com)
Resistance2
1990 Civilizations had been co-transfected using SuperFect (Qiagen) with vectors having a puromycin level of resistance gene with a TCF luciferase reporter (TopTK) (Brantjes et al . (bioxorio.com)
After selection for puromycin level of resistance cells had been contaminated with retroviruses (presents of A. R and kisper. (bioxorio.com)
Selection1
Commonly used in genetic selection experiments using neomycin as selection agent. (sigmaaldrich.com)
Cells2
Neomycin resistant MEF cells isolated from FVB mouse embryos at day 13. (sigmaaldrich.com)
Acting as an analog of the 3′ terminal end of aminoacyl-tRNA, puromycin incorporates itself into a growing polypeptide chain and causes its premature termination, thereby inhibiting protein synthesis. (canvaxbiotech.com)
It is composed of neomycins A, B, and C, and acts by inhibiting translation during protein synthesis. (nih.gov)