• Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
  • During embryonic myogenesis, migration of skeletal myogenic progenitors is essential to generate the anlagen of limbs, diaphragm and tongue, whereas in post-natal skeletal muscles, migration of muscle satellite (stem) cells towards regions of injury is necessary for repair and regeneration of muscle fibres. (crick.ac.uk)
  • This occurs during myogenesis with the fusion of myoblasts each contributing a nucleus to the newly formed muscle cell or myotube. (wikipedia.org)
  • Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
  • Myogenesis is a multi-step process through which uncommitted mesodermal cells commit, differentiate and undergo profound phenotypic changes. (biologists.com)
  • Hence, in order to unravel the functions of circRNAs in myogenesis, the present study explored circRNA profiling in skeletal muscle between Mashen and Large White pigs. (bvsalud.org)
  • Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
  • The regulation of SC activity is governed by myogenic regulatory factors (MRF), including MyoD, Myf5, myogenin, and MRF4. (mcmaster.ca)
  • NEW & NOTEWORTHY The myogenic program is controlled via transcription factors referred to as myogenic regulatory factors (MRF). (mcmaster.ca)
  • The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)
  • Studies previously conducted show that HS negatively affects the skeletal muscle growth and development by changing its effects on myogenic regulatory factors, insulin growth factor-1, and heat-shock proteins. (frontiersin.org)
  • A number of different local transcription factors called myogenic regulatory factors (MRFs), including MyoD, myf-5, myogenin, and myf-6, stimulate proliferation and differentiation of satellite cells [ 9 ]. (hindawi.com)
  • Head and trunk muscles have discrete embryological origins and are governed by distinct regulatory programmes. (ncbs.res.in)
  • They also showed that cross-regulation between col and eya in muscle progenitor cells is required for specification of muscle identity, revealing a new parallel between the myogenic regulatory networks operating in Drosophila and vertebrates. (sdbonline.org)
  • Of particular importance has been the identification of two families of transcription factors: the myogenic regulatory factors (MRFs) and the myocyte enhancer factors (MEFs). (biologists.com)
  • Directional cell migration is a critical process underlying morphogenesis and post-natal tissue regeneration. (crick.ac.uk)
  • Here, we review mechanisms of myogenic stem/progenitor cell migration during skeletal muscle development and post-natal regeneration. (crick.ac.uk)
  • The main interest of Dr. Darabi's lab is to use pluripotent stem cells for skeletal muscle regeneration in muscular dystrophies and muscle injuries. (uh.edu)
  • 2012) Age dependent increase in the levels of osteopontin inhibits skeletal muscle regeneration. (ca.gov)
  • Injection of FGF-6 recombinant protein into damaged mouse muscle can induce cyclin D1 mRNA and differentiation markers, including Cdkl1, MHC I and Tn1, which results in the acceleration of muscle regeneration. (biolegend.com)
  • During muscle regeneration, it has been shown that FGF-6 can be released from necrotic myofibers and be sequestered by basal laminae. (biolegend.com)
  • In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
  • Our current running hypothesis is that the positive growth factors produced by hESCs trigger injured muscle to initiate and maintain regeneration, the TGF-beta inhibitors produced by hESCs reduce the TGF-beta signaling, and the combination assures the robust regeneration of muscle. (ca.gov)
  • Preliminary data indicates the effects on regeneration of old muscle look very promising. (ca.gov)
  • Specifically, our data suggest that several other ligands of MAPK pathway secreted by hESCs are likely to enhance and rejuvenate the regeneration of old muscle tissue. (ca.gov)
  • We have submitted the several manuscripts on topics of enhanced tissue regeneration and we are preparing the manuscript that identifies hESC-based novel strategies for restoring high regenerative capacity to old muscle. (ca.gov)
  • Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
  • However, HIF1α/2α dKO in postnatal satellite cells impairs injury-induced muscle repair, accompanied by a reduced number of myoblasts during regeneration. (purdue.edu)
  • Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
  • More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
  • Cellular and molecular regulation of muscle regeneration. (scielo.br)
  • Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
  • Regeneration of muscle after injury normally occurs rapidly with satellite stem cells that reside in the space between the basal lamina and the sarcolemma, playing a major role [ 11 ]. (hindawi.com)
  • In this study, we investigated the effect of catechins, polyphenol compounds in green tea, on muscle regeneration. (korea.ac.kr)
  • Finally, EGCG administration to mice significantly increased muscle fiber size for regeneration. (korea.ac.kr)
  • Taken together, the results suggest that catechins stimulate muscle stem cell activation and differentiation for muscle regeneration. (korea.ac.kr)
  • Wu J, Matthias N, Lo J, Ortiz-Vitali JL, Shieh AW, Wang SH, Darabi R . A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. (uh.edu)
  • Human ES and iPS-Derived Myogenic Progenitors Restore Dystrophin and Improve Contractility upon Transplantation in Dystrophic Mice. (uh.edu)
  • Assessment of the myogenic stem cell compartment following transplantation of pax3/pax7-induced embryonic stem cell-derived progenitors. (uh.edu)
  • Next, surface marker screen allowed identification of CD10 and CD24 for purification of myogenic progenitors. (nih.gov)
  • CD10 expression was also confirmed on human satellite cells and muscle progenitors. (nih.gov)
  • Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)
  • Remarkably, the progenitors derived from embryonic stem cells by dual inhibition efficiently differentiate into cardiac and skeletal muscle cells. (ncbs.res.in)
  • Significantly, this is the first report of directed differentiation of pluripotent stem cells, without transgenes, into progenitors with muscle/heart dual potential. (ncbs.res.in)
  • describe a protocol for the direct reprogramming of mouse fibroblasts into expandable functional skeletal muscle progenitors. (tocris.com)
  • 2018) Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors. (tocris.com)
  • In the lymph gland , an hematopoietic organ in Drosophila larva, a group of cells called the Posterior Signaling Centre (PSC), whose specification depends on the EBF transcription factor Collier (Col) and the HOX factor Antennapedia (Antp), has been proposed to form a niche required to maintain the pool of hematopoietic progenitors (prohemocytes). (sdbonline.org)
  • In contrast with this model, this study shows that genetic ablation of the PSC does not cause an increase in blood cell differentiation or a loss of blood cell progenitors. (sdbonline.org)
  • Collier is expressed in a core population of lymph gland progenitors and cell autonomously maintains this population. (sdbonline.org)
  • The PSC contributes to lymph gland homeostasis by regulating blood cell differentiation, rather than by maintaining core progenitors. (sdbonline.org)
  • Here, we show that the muscle-specific determination genes myf5. (uni-luebeck.de)
  • In situ hybridization demonstrates transcripts for myogenin and myoD accumulating in mono- and multinucleated myogenic cells, while myf5 mRNA is already found in mononucleated myoblasts. (uni-luebeck.de)
  • Wu J, Hunt SD, Xue H, Liu Y, Darabi R . Generation and Characterization of a MYF5 Reporter Human iPS Cell Line Using CRISPR/Cas9 Mediated Homologous Recombination. (uh.edu)
  • hESCs were analyzed at 5 differentiation time-points (Day0, Day4, Day10-sorted for MYF5 EGFP positive or negative- and day20 myotubes after myogenic differentiation of MYF5 EGFP positive fraction). (nih.gov)
  • To compare transcriptome profile of PAX7/MYF5 double reporter hESC derived myogenic cells to human skeletal myoblasts, we enlisted ENCODE polyA RNA-Seq data of the human skeletal muscle myoblasts from the Wold lab (GEO: GSM958744). (nih.gov)
  • Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)
  • We found that (−)-epicatechin gallate (ECG) and (−)-epigallocatechin-3-gallate (EGCG) activate satellite cells by induction of Myf5 transcription factors. (korea.ac.kr)
  • On day 0, fibroblast culture medium was exchanged for induced myogenic progenitor cell (iMPCs) medium, which resulted in iMPCs expressing key skeletal muscle stem and progenitor cell markers, by day 19. (tocris.com)
  • The maintenance of stem or progenitor cell fate relies on intrinsic factors as well as local cues from the cellular microenvironment and systemic signaling. (sdbonline.org)
  • The mouse blastocyst-derived embryonic stem cell (ES cell) line BLC6 efficiently differentiates into myosin heavy chain-, desmin- and myogenin- positive skeletal muscle cells when cultivated in embryo-like aggregates (embryoid bodies). (uni-luebeck.de)
  • Myogenin also identified as myogenic factor 4 is a muscle-specific transcription factorassociated with muscle differentiation and cell cycle. (sigmaaldrich.com)
  • Anti-myogenin reactivity isseen in the nuclei of myoblasts in developing muscle tissue. (sigmaaldrich.com)
  • ECG also promotes myogenic differentiation through the induction of myogenic markers, including Myogenin and Muscle creatine kinase (MCK), in satellite and C2C12 myoblast cells. (korea.ac.kr)
  • At higher concentrations, FGF-6 can downregulate the expression of myogenic markers and some myogenic transcription factors, and can delay C2 myogenic cell differentiation. (biolegend.com)
  • With GemCode, cells are captured in droplets, and the reverse transcription that occurs within each droplet is used to generate barcoded cDNA. (genengnews.com)
  • The EBF transcription factor Collier directly promotes Drosophila blood cell progenitor maintenance independently of the niche. (sdbonline.org)
  • Together, this study shows that the PSC is dispensable for blood cell progenitor maintenance and reveals the key role of the conserved transcription factor Col as an intrinsic regulator of hematopoietic progenitor fate. (sdbonline.org)
  • Collier, the single Drosophila COE (Collier/EBF/Olf-1) transcription factor, is required in several developmental processes, including head patterning and specification of muscle and neuron identity during embryogenesis. (sdbonline.org)
  • this requires coordination of myogenic transcription factors. (bvsalud.org)
  • Myogenic transcription is tightly regulated by various intracellular signaling pathways, which include members of the protein kinase D (PKD) family. (bvsalud.org)
  • Muscle cells (including myocytes and muscle fibers) develop from embryonic precursor cells called myoblasts. (wikipedia.org)
  • Component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells. (nih.gov)
  • It has been shown that the expression of FGF-6 can transiently be upregulated by muscle injuries in both fast and slow myofibers. (biolegend.com)
  • Analysis of satellite cell dynamics on myofibers confirms that HIF1α/2α dKO myoblasts exhibit reduced self-renewal but more pronounced myogenic differentiation under hypoxia conditions. (purdue.edu)
  • When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
  • Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
  • The mRNA expression of these MRF in humans following muscle damage has been predominately measured in whole muscle homogenates. (mcmaster.ca)
  • In conclusion, our results suggest that the directional changes in mRNA expression of the MRF in a whole muscle homogenate in response to acute eccentric exercise reflects that observed in isolated muscle SC. (mcmaster.ca)
  • Previous studies have derived MRF expression from whole muscle homogenates, but little work has examined whether the mRNA expression of these transcripts reflects the pattern of expression in the actual population of satellite cells (SC). (mcmaster.ca)
  • FGF-6 mRNA can also be detected in testis, heart, and skeletal muscle in adults. (biolegend.com)
  • In a recent study, Dr. Hindson and colleagues described 10x Genomics' GemCode technology, a droplet-based platform that combines microfluidics with molecular barcoding and custom bioinformatics software to enable 3′ mRNA counting from thousands of single cells. (genengnews.com)
  • embryologically, this multinucleate condition results from multiple myoblasts fusing to produce each muscle fiber, where each myoblast contributes one nucleus. (wikipedia.org)
  • Cells called myoblasts elongate and fuse together at their tips to make the muscle cells. (elifesciences.org)
  • The experiments show that the filaments progressively become more ordered as the myoblasts develop into muscle cells. (elifesciences.org)
  • identified a new member of the MAP family that is produced in myoblasts as soon as they start to form muscle fibres, and named it oMAP4. (elifesciences.org)
  • Upon differentiation, mono-nucleated myoblasts withdraw from the cell cycle and fuse to form syncytial myotubes ( Wakelam, 1985 ). (elifesciences.org)
  • Ensure high yields when passaging and culturing your primary human skeletal muscle progenitor cells (myoblasts) by using MyoCult™-SF Attachment Substrate, which is optimized to support myoblast attachment. (stemcell.com)
  • human pluripotent stem cell (hPSC)-derived myoblasts may be cultured on cultureware coated with Corning® Matrigel® hESC-Qualified Matrix. (stemcell.com)
  • However, the in vivo roles of HIFs in quiescent satellite cells and activated satellite cells (myoblasts) are poorly understood. (purdue.edu)
  • Interestingly, double knockout of HIF1α and HIF2α (HIF1α/2α dKO) in embryonic myoblasts results in apparently normal muscle development and growth. (purdue.edu)
  • Skeletal muscle satellite cells (SC) play an important role in muscle repair following injury. (mcmaster.ca)
  • In December 2014, he successfully defended his Ph.D. thesis entitled " Satellite cells in skeletal muscle atrophy and hypertrophy " (Maastricht University). (maastrichtuniversity.nl)
  • Muscle satellite cells are myogenic stem cells whose quiescence, activation, self-renewal and differentiation are influenced by microenvironment oxygen levels. (purdue.edu)
  • To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
  • Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)
  • When activated by injury, satellite cells reenter the cell cycle and proliferate in response to extracellular growth factors ( Relaix & Zammit, 2012 Relaix F, Zammit PS. (scielo.br)
  • MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
  • The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
  • Satellite cells, the engines of muscle repair. (scielo.br)
  • Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
  • In addition, insulin, such as growth factor (IGF) 1Ea and/or IGF-1Ec (MGF), also stimulates proliferation and differentiation of satellite cells. (hindawi.com)
  • Functional assays showed that circIGF1R promoted myoblast differentiation of porcine skeletal muscle satellite cells (SMSCs), while it had no effect on cell proliferation. (bvsalud.org)
  • During the last several years, his lab has developed novel methods for multi-lineage differentiation of pluripotent stem cells for cell therapy in mice models of muscular dystrophies and injuries. (uh.edu)
  • Derivation of Skeletal Myogenic Precursors from Human Pluripotent Stem Cells Using Conditional Expression of PAX7. (uh.edu)
  • One recently-completed CIRM-funded project involved directed evolution and engineering of new viral gene delivery vehicles capable of highly efficient delivery to human embryonic and induced pluripotent stem cells. (ca.gov)
  • 2012) "Directed Evolution of Adeno-Associated Virus for Enhanced Gene Delivery and Gene Targeting in Human Pluripotent Stem Cells. (ca.gov)
  • 2012) "Soft Microenvironments Promote the Early Neurogenic Differentiation but not Self-renewal of Human Pluripotent Stem Cells. (ca.gov)
  • This study elucidated effects of glutamine (Gln) supplementation on the cellular muscle development of LBW and normal birth weight (NBW) piglets. (nature.com)
  • We observed a temporarily increased intramuscular availability of free Gln that could have influenced cellular processes in skeletal muscle. (nature.com)
  • Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
  • Hypoxia inducible factors (HIFs) are central mediators of cellular responses to fluctuations of oxygen, an environmental regulator of stem cell activity. (purdue.edu)
  • Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
  • In a proof-of-concept analysis that used 68,000 fresh peripheral blood mononuclear cells obtained from a healthy donor, Dr. Hindson and colleagues showed that GemCode can be used to interrogate cellular heterogeneity and cluster large immune cell populations. (genengnews.com)
  • The same proportions of cellular subpopulations were also captured from frozen cells, indicating that this method can be used on frozen samples. (genengnews.com)
  • Removal of PrPSc replication in SMB-S15 cells reversed the upregulation of cellular Gal-3, TREM2, and the relevant proteins. (bvsalud.org)
  • Initial analysis of Collier/Early B Cell Factor function in the lymph gland revealed the role of the Posterior Signaling Center (PSC) in mounting a dedicated cellular immune response to wasp parasitism. (sdbonline.org)
  • Control of contractile protein synthesis in the extraocular muscles: Although oculomotor disorders such as strabismus or nystagmus can have marked, deleterious effects on the development of vision, little is known about the final common pathway of oculomotor control: the extraocular muscles (EOMs). (upenn.edu)
  • Promotes differentiation of myogenic cells. (nih.gov)
  • Here we show that, concurrent with a decrease in chondrogenesis, inhibition of p38 in limb bud cultures dramatically promotes muscle development. (biologists.com)
  • Furthermore, strategies utilised to improve migratory capacity of myogenic cells are examined in order to identify potential treatments that may be applied to future transplantation protocols. (crick.ac.uk)
  • Clare's lab recently identified a population of progenitor/stem cells in mice that, on transplantation, is sufficient to generate a fully functional thymus. (eurostemcell.org)
  • There are three types of myofilaments: thin, thick, and elastic that work together to produce a muscle contraction. (wikipedia.org)
  • The thin myofilaments are filaments of mostly actin and the thick filaments are of mostly myosin and they slide over each other to shorten the fiber length in a muscle contraction. (wikipedia.org)
  • in order for the heart to function properly as a whole, the contraction of all its constituent cells must be coordinated. (todayifoundout.com)
  • This entire contraction phase is known as systole, and is followed by a second phase known as diastole in which the cardiac muscle relaxes, allowing blood to flow into the atria. (todayifoundout.com)
  • Unlike skeletal muscle , which contracts in response to nerve stimulation, and like single unit smooth muscle, cardiac muscle is myogenic , meaning that it is self-excitable stimulating contraction without a requisite electrical impulse coming from the central nervous system. (wikidoc.org)
  • If synchronization of cardiac muscle contraction is disrupted for some reason (for example, in a heart attack ), uncoordinated contraction known as fibrillation can result. (wikidoc.org)
  • Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)
  • The mesenchyme that fills the pharyngeal arches is derived from the following 3 origins: the paraxial mesoderm, the lateral plate mesoderm, and the neural crest cells. (medscape.com)
  • Although paraxial mesoderm and lateral plate mesoderm contribute to the musculature that develops in each particular arch, neural crest cells contribute to the skeletal portion of each arch. (medscape.com)
  • The microtubule cytoskeleton is critical for muscle cell differentiation and undergoes reorganisation into an array of paraxial microtubules, which serves as template for contractile sarcomere formation. (elifesciences.org)
  • Most notably, oMAP4 is required for paraxial microtubule organisation in muscle cells and prevents dynein- and kinesin-driven microtubule-microtubule sliding. (elifesciences.org)
  • Here, we show that, unlike the myogenic trunk paraxial mesoderm, head mesoderm development is independent of the T/Tbx6 network in mouse. (ncbs.res.in)
  • These cells originate from the paraxial mesoderm and migrate into the facial primordia. (medscape.com)
  • More proliferation was observed in muscle tissue of LBW-GLN than LBW-ALA piglets at 5 dpn, but there was no clear effect of supplementation on related gene expression. (nature.com)
  • The Human Cell Atlas relies, to a great extent, on the use of transcriptome sequencing to profile the gene expression of individual cells. (genengnews.com)
  • We developed a fast way of profiling tens of thousands of cells, where for each cell, we capture gene expression in an unbiased way," says Benjamin J. Hindson, Ph.D., CSO, president, and cofounder of 10x Genomics. (genengnews.com)
  • Cell Ranger is a set of analysis pipelines that processes Chromium single-cell 3′ RNA-Seq output to align reads, generate gene-cell matrices, and perform clustering and gene-expression analysis. (genengnews.com)
  • PKD is a family of serine-threonine kinases that regulate gene expression, protein secretion, cell proliferation, differentiation and inflammation. (bvsalud.org)
  • As class I HDACs and PKD signaling are requisite for myocyte differentiation, these data suggest that HDAC8 functions as a potential feedback regulator of PKD phosphorylation to control myogenic gene expression. (bvsalud.org)
  • A striated muscle fiber contains myofibrils consisting of long protein chains of myofilaments. (wikipedia.org)
  • His studies were focused on the identification of different exercise and nutritional intervention strategies to further augment skeletal muscle protein synthesis and muscle satellite cell function in older adults. (maastrichtuniversity.nl)
  • The microtubules in cells that make smaller amounts of this protein were more disorganised, and these cells were unable to fuse with each other to form muscle cells. (elifesciences.org)
  • 2013) "A Modular Optogenetic Platform for Inducible Protein Clustering and Signaling Activation in Mammalian Cells. (ca.gov)
  • Therefore, in collaboration with W. Wright, we have developed an approach for the immortalisation of human muscle precursors that allows them to retain their ability to differentiate: this has enabled the generation of immortal cell lines derived from patient cells. (institut-myologie.org)
  • As an assistant Professor at Maastricht University, he is now involved in both research and education focused on the skeletal muscle fiber perfusion in muscle adaptive response to exercise training in young, old and more compromised clinical populations. (maastrichtuniversity.nl)
  • However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
  • The individual facial primordia are populated by neural crest cell populations that arise in different regions of the head neural folds. (medscape.com)
  • Glutamine is regarded as the most abundant amino acid in the body, is mainly synthesized in skeletal muscle 10 , and muscle tissue is the most important site for Gln storage 11 . (nature.com)
  • Skeletal muscle cells and cardiac muscle cells both contain myofibrils and sarcomeres and form a striated muscle tissue. (wikipedia.org)
  • The neural crest cells give rise to the connective tissue components, including cartilage, bone, and ligaments in the facial and oral regions. (medscape.com)
  • Skeletal muscle is the largest tissue in the body. (frontiersin.org)
  • 43 Therefore, cell sheets with enhanced thickness and mechanical properties obtained through appropriate cyclic mechanical stimulation possess great potential for bone tissue engineering applications. (researchgate.net)
  • Cell sheet-based scaffold-free technology holds promise for tissue engineering applications and has been extensively explored during the past decades. (researchgate.net)
  • Findings from this study reveal that thermo-responsive elastomer, together with mechanical conditioning, can potentially be applied to prepare high-quality cell sheets for bone tissue engineering. (researchgate.net)
  • Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
  • Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
  • Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
  • This transmission of impulses makes cardiac muscle tissue similar to nerve tissue, although cardiac muscle cells are notably connected to each other by intercalated discs . (wikidoc.org)
  • SFTs of the pleura are localized mesenchymal neoplasms composed of fibroblastlike cells believed to arise from the subpleural connective tissue. (medscape.com)
  • Light microscopy and transmission electron microscopy of left biceps branchii muscle biopsy tissue from a 45-year-old man with microsporidiosis caused by Anncaliia algerae , China. (cdc.gov)
  • Additionally, our data in progress suggest that muscle and brain age by similar molecular mechanisms and thus, therapeutic strategies for rejuvenating muscle repair might be applicable to the restoration of neurogenesis in aged brain. (ca.gov)
  • He has been leader of a research group at the Max-Delbrück-Center for Molecular Medicine in Berlin until 2011 focusing his research on signal transduction mechanisms in human and murine embryonic stem cells. (eurostemcell.org)
  • These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)
  • In this study, we investigated the effect of vindoline, an indole alkaloid of Catharanthus roseus on insulin resistance (IR), oxidative stress and inflammatory responses in dexamethasone (IR inducer)-induced dysfunctional 3T3-L1 adipocytes and high-glucose-induced insulin-resistant L6-myoblast cells. (bvsalud.org)
  • Thus, in vitro studies of vindoline in insulin resistant skeleton muscle and dysfunctional adipocytes confirmed that vindoline treatment significantly mitigated insulin resistance in myotubes and improved functional status of adipocytes. (bvsalud.org)
  • These results demonstrated that vindoline has the potential to be used as a therapeutic agent to ameliorate obesity-induced T2DM-associated insulin resistance profile in adipocytes and skeletal muscles. (bvsalud.org)
  • In vivo studies using transgene/reporter-free hESC/iPSCs further validated myogenic potential of the cells by dystrophin restoration and satellite cell engraftment in NSG-mdx4cv mice. (nih.gov)
  • Our team has demonstrated some important differences between man and mouse, in the regulation of the proliferation of myogenic precursors. (institut-myologie.org)
  • Although administration of various myogenic cell types has been done intramuscularly or intravascularly, functional restoration has not been achieved yet in patients with degenerative diseases affecting multiple large muscle. (crick.ac.uk)
  • The myocytes also express functional nicotinic cholinoceptors and exhibit T-type Ca 2+ currents and later L-type Ca 2+ currents, demonstrating physiological properties of skeletal muscle cells. (uni-luebeck.de)
  • He co-ordinates the EU Horizon2020 funded stem cell research consortium - INTENS which aims to aims to make a functional reconstructed bowel for people with Short Bowel Syndrome. (eurostemcell.org)
  • Aging is related to changes in muscle quantity and quality [ 1 ], both of which have important implications for functional performance [ 2 ]. (hindawi.com)
  • These adverse changes in muscle quantity, muscle quality, and the resulting functional deficits are referred to under a common name of "sarcopenia. (hindawi.com)
  • For the purposes of this paper, we define sarcopenia as originally defined by Rosenberg [ 3 ], an age-related loss of muscle, and view the functional aspects of the most recent definitions as consequences of sarcopenia. (hindawi.com)
  • These changes include elongation, polarization, aggregation and fusion, which ultimately give rise to functional muscle. (biologists.com)
  • At lower concentration, FGF-6 can increase the expression of many muscle cell differentiation markers. (biolegend.com)
  • Specifically,treatment of primary limb bud cultures with p38 inhibitors increases the expression of myogenic markers and causes a striking increase in formation of myotubes, which were detected using antibodies specific for myosin heavy chain. (biologists.com)
  • Skoglund G, Lainé J, Darabi R , Fournier E, Perlingeiro R, Tabti N. Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. (uh.edu)
  • Project Title: The Berkeley Human Embryonic Stem Cell Shared Research Laboratory a. (ca.gov)
  • FGF-6 is involved in both proliferation and differentiation of the myogenic lineages. (biolegend.com)
  • Col regulation of eya , both in specific muscle and neuronal lineages, may illustrate one mechanism behind the evolutionary diversification of Col biological roles. (sdbonline.org)
  • Paolo De Coppi is a Consultant Paediatric Surgeon at Great Ormond Street Hospital (GOSH), and Reader and Head of Stem Cells and Regenerative Medicine at the UCL Institute of Child Health in London. (eurostemcell.org)
  • Independent regulation of tumor cell migration by matrix stiffness and confinement. (ca.gov)
  • Expression of muscle growth related genes was quantified with qPCR. (nature.com)
  • These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
  • abstract = "Muscle weakness is one of the most common symptoms in aged individuals and increases risk of mortality. (korea.ac.kr)
  • Our results suggest that ES cells recapitulate closely the early steps of muscle development in vivo and may serve as an excellent in vitro system to study this process. (uni-luebeck.de)
  • Molecular biology of the cell. (kcl.ac.uk)
  • Nature Reviews Molecular Cell Biology 2012;13(2):127-33. (scielo.br)
  • We publish articles in pharmaceutical sciences and related disciplines (including biotechnology, cell and molecular biology, drug utilization including adverse drug events, medical and other life sciences, and related engineering fields). (ajol.info)
  • There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
  • Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
  • Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
  • Additionally, safe and efficient migration of transplanted cells is critical in cell therapies, both allogeneic and autologous. (crick.ac.uk)
  • One of the key reasons for this negative outcome is the limited migration of donor cells, which hinders the overall cell engraftment potential. (crick.ac.uk)
  • Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
  • PKD is a unique PKC family member that shares distant sequence homology to calcium-regulated kinases and plays an important role in muscle physiology. (bvsalud.org)
  • For more information on protocols for the derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet . (stemcell.com)
  • After implantation into the critical-sized calvarial defects of mice, the mechanically conditioned cell sheets significantly promoted new bone formation. (researchgate.net)
  • Matthias N, Hunt SD, Wu J, Darabi R . Skeletal muscle perfusion and stem cell delivery in muscle disorders using intra-femoral artery canulation in mice. (uh.edu)
  • Mice deficient in Notch3 have defects in arterial vascular smooth muscle cell (VSMC) mechanosensitivity, including impaired myogenic responses and autoregulation, and inappropriate VMSC orientation. (cdc.gov)
  • Published in the journal Proceedings of the National Academy of Sciences , the study, which was carried out in mice, reveals that hypertension disrupts signalling within artery cells in the brain and leads to vascular dementia. (hospitalhealthcare.com)
  • Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)
  • Furthermore, the rescue experiments showed that circIGF1R could counteract the inhibitory effect of miR-16 on cell myoblast differentiation. (bvsalud.org)
  • 6- Differentiation of iPSCs into cardiomyocytes and endothelial cells for disease modeling and screening. (uh.edu)
  • Myogenic differentiation of iPSCs has been done by gene-overexpression or directed differentiation. (nih.gov)
  • This strategy allowed pathway screen to define efficient myogenic induction in hESC/iPSCs. (nih.gov)
  • If age-imposed decline in the regenerative capacity of stem cells was understood, the debilitating lack of organ maintenance in the old could be ameliorated and perhaps, even reversed. (ca.gov)
  • Whether the temporal expression of MRF in a whole muscle homogenate reflects SC-specific expression of MRF remains largely unknown. (mcmaster.ca)
  • We report that MRF expression from an enriched SC population reflects the directional pattern of expression from skeletal muscle biopsy samples following eccentric contractions. (mcmaster.ca)
  • FGF-6 exhibits a very restricted expression profile predominantly in the developing muscle in embryos. (biolegend.com)
  • potent anti-cancer drugs, combretastatin A-4 (CA-4) and docetaxel (DTX), against tumor cells and vasculature. (ajol.info)
  • Medulloblastoma is defined by the World Health Organization (WHO) as "an embryonal neuroepithelial tumor arising in the cerebellum or dorsal brainstem, presenting mainly in childhood and consisting of densely packed small round undifferentiated cells with mild to moderate nuclear pleomorphism and high mitotic count. (medscape.com)
  • In diabetes, more fat and less carbohydrate is used, due to the reduced induction of GLUT4 glucose transporters to the cell surfaces. (wikidoc.org)