Myogenic human skeletal muscle satellite cells skeletal muscle. Medical search. Frequent questions

The Wnt signaling pathway is involved in the control of cell proliferation and differentiation during skeletal muscle development. (biomedcentral.com)
Myogenic differentiation of iPSCs has been done by gene-overexpression or directed differentiation. (nih.gov)
This study provides new biological insights for myogenic differentiation using a new cell resource and identifies CD10 as a potential myogenic marker. (nih.gov)
hESCs were analyzed at 5 differentiation time-points (Day0, Day4, Day10-sorted for MYF5 EGFP positive or negative- and day20 myotubes after myogenic differentiation of MYF5 EGFP positive fraction). (nih.gov)
During the last several years, his lab has developed novel methods for multi-lineage differentiation of pluripotent stem cells for cell therapy in mice models of muscular dystrophies and injuries. (uh.edu)
These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
6- Differentiation of iPSCs into cardiomyocytes and endothelial cells for disease modeling and screening. (uh.edu)
The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)
MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)
While acknowledging progress in the other areas mentioned, this work will focus on the current debates concerning sourcing, MSC alterations of angiogenesis, cell differentiation/stimulation, and strategies to improve MSC differentiation. (biomedcentral.com)
Serra M, Brito C and Alves PM. "Bioengineering strategies for stem cell expansion and differentiation. (sciepub.com)
Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K. "Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. (sciepub.com)
The effects of the isolated EVs on human satellite cell proliferation and differentiation and macrophage activation were examined.Results Exosomes from IM patients decreased human satellite cell proliferation (51%, P<0.01) and inhibited their myogenic differentiation as indicated by lower fusion index (24% inhibition, P<0.01) and expression of myosin heavy chain (72% inhibition, P<0.001). (biu.ac.il)
Another significant difference was in the expression of signaling molecules: Thus, exosomes from BMD patients increased the phosphorylation of Erk and p38, whereas a smaller effect was induced by IM exosomes.Conclusion Exosomes from IM patients decrease satellite cell proliferation and myogenic differentiation compared to healthy exosomes. (biu.ac.il)
Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation. (bioseek.eu)
Conversely, ectopic expression of PRDM16 in myoblasts induces their differentiation into brown fat cells. (bioseek.eu)
The manipulation of fat stores is an obvious therapeutic dream, but disruption of the normal differentiation or development of white adipose tissues (WAT) causes ectopic lipid storage and severe pathology (lipodystrophy) in both humans and experimental animals. (bioseek.eu)
Their fate is regulated by the expression of various transcription factors, including paired box 7 (Pax7) and myogenic differentiation (MyoD) [ 6 ]. (biomedcentral.com)
All cells have been tested for myotube differentiation. (zen-bio.com)
Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. (zen-bio.com)
Myogenic differentiation was verified by immunohistochemical staining using antibodies to desmin and myosin. (zen-bio.com)
Specially formulated media are available for maintenance and differentiation of our adult skeletal myoblasts. (zen-bio.com)

Direct isolation of satellite cells for skeletal muscle regeneration. (medecinesciences.org)
Alameddine HS, Dehaupas M, Fardeau M. Regeneration of skeletal muscle fibers from autologous satellite cells multiplied in vitro. (medecinesciences.org)
Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration. (medecinesciences.org)
Several approaches were done to prevent muscle soreness and stimulate regeneration. (hindawi.com)
The main interest of Dr. Darabi's lab is to use pluripotent stem cells for skeletal muscle regeneration in muscular dystrophies and muscle injuries. (uh.edu)
One of the great advantages of tissue engineering of skeletal muscle is that adult skeletal muscle contains many myogenic precursor stem cells, termed "satellite cells", which are involved in the repair and regeneration of skeletal muscle throughout the lifespan. (josam.org)
Additionally, muscles of heterozygous HIV-1 transgenic mice displayed increased inflammation and blunted myofiber growth in an injury-induced muscle regeneration process. (imrpress.com)
Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
Cellular and molecular regulation of muscle regeneration. (scielo.br)
Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
Our current running hypothesis is that the positive growth factors produced by hESCs trigger injured muscle to initiate and maintain regeneration, the TGF-beta inhibitors produced by hESCs reduce the TGF-beta signaling, and the combination assures the robust regeneration of muscle. (ca.gov)
Preliminary data indicates the effects on regeneration of old muscle look very promising. (ca.gov)
Specifically, our data suggest that several other ligands of MAPK pathway secreted by hESCs are likely to enhance and rejuvenate the regeneration of old muscle tissue. (ca.gov)
We have submitted the several manuscripts on topics of enhanced tissue regeneration and we are preparing the manuscript that identifies hESC-based novel strategies for restoring high regenerative capacity to old muscle. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
Directional cell migration is a critical process underlying morphogenesis and post-natal tissue regeneration. (crick.ac.uk)
During embryonic myogenesis, migration of skeletal myogenic progenitors is essential to generate the anlagen of limbs, diaphragm and tongue, whereas in post-natal skeletal muscles, migration of muscle satellite (stem) cells towards regions of injury is necessary for repair and regeneration of muscle fibres. (crick.ac.uk)
Here, we review mechanisms of myogenic stem/progenitor cell migration during skeletal muscle development and post-natal regeneration. (crick.ac.uk)
In particular, her work has been focusing on the molecular mechanisms involved in the modulation of the peripheral synaptic transmission, skeletal muscle adaptation and regeneration in physiological and pathological conditions. (units.it)
The research activity of the group is focused on the identification of new strategies to improve skeletal muscle regeneration. (units.it)
Therefore, medications that can prevent the progression of muscle atrophy or promote muscle regeneration are in high demand. (biomedcentral.com)
Considering that reinnervation of the TA muscle can occur even after UVFP through these anastomoses, it would be ideal if drugs that promote nerve regeneration could be used to treat UVFP. (biomedcentral.com)
Satellite cells (SCs), which are precursors of skeletal muscles, such as the TA muscle, play a key role in muscle homeostasis and regeneration. (biomedcentral.com)
SCs are normally in a quiescent state in adult muscles, and start proliferating during muscle regeneration in response to denervation. (biomedcentral.com)
Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
We investigated the hypothesis that specific energy levels of LIPUS mediates skeletal muscle regeneration by modulating the inflammatory microenvironment. (ijbs.com)
LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)

MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. (biomedcentral.com)
We have developed a comprehensive profile of human myogenesis using single cell sequencing. (ca.gov)
We will perform single cell RNA sequencing (scRNA-seq) of embryonic, fetal, juvenile and adult muscle stem cells (MuSCs) and identify unique gene signatures and cell states across human myogenesis. (ca.gov)
Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
Myogenesis is a multi-step process through which uncommitted mesodermal cells commit, differentiate and undergo profound phenotypic changes. (biologists.com)
The past two decades has seen major advances towards our current understanding of skeletal myogenesis (reviewed in Perry and Rudnicki, 2000 ). (biologists.com)
In more detail, the team is studying the role of electrical activity and local trophic factors in the microenvironment of satellite cell niche and in the post-mitotic myogenesis. (units.it)

In December 2014, he successfully defended his Ph.D. thesis entitled " Satellite cells in skeletal muscle atrophy and hypertrophy " (Maastricht University). (maastrichtuniversity.nl)
On the other hand, despite the atrophy and reduced myogenic potential of the EDL muscles in old rats, when an EDL muscle is cross-age transplanted from an old donor rat into a young host rat, the muscle regenerates as much mass and has as high a capacity for force development as a young muscle transplanted into a young host [4] . (josam.org)
There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
In this study, we identified a selective atrophy of fast-glycolytic myofibers in skeletal muscles of homozygous HIV-1 transgenic mice, whereas the more oxidative fiber types are spared. (imrpress.com)
Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
We previously reported that local administration of bFGF 1 month after recurrent laryngeal nerve (RLN) paralysis compensated for atrophy of the thyroarytenoid muscle. (biomedcentral.com)
As increased neuromuscular junctions are expected to maintain myofiber volume, bFGF administration may prevent thyroarytenoid muscle atrophy in the mid to long term. (biomedcentral.com)
These treatments provide only static adjustment to the larynx, and their effects may be limited by the remaining hoarseness due to progressive atrophy of the paralyzed thyroarytenoid (TA) muscle. (biomedcentral.com)
In lung diseases, muscle mass usually decreases and severe atrophy occurs. (jeoct.com)
This assumption long seemed valid, with many researchers reporting the presence of disintegrating nuclei in muscle tissue during atrophy induced by inactivity, injury or paralysis. (robert-gorter.info)
Muscles get damaged during extreme exercise, and often have to weather changes in food availability and other environmental factors that lead to atrophy. (robert-gorter.info)
Since myostatin and follistatin bind to the same receptor, the follistatin to myostatin (F:M) ratio is associated with muscle atrophy or hypertrophy [ 12 ]. (biomedcentral.com)

Transplanted myoblasts can migrate several millimeters to fuse with damaged myofibers in nonhuman primate skeletal muscle. (medecinesciences.org)
Long-term engraftment (16 years) of myoblasts in a human infarcted heart. (medecinesciences.org)
To compare transcriptome profile of PAX7/MYF5 double reporter hESC derived myogenic cells to human skeletal myoblasts, we enlisted ENCODE polyA RNA-Seq data of the human skeletal muscle myoblasts from the Wold lab (GEO: GSM958744). (nih.gov)
Satellite cells ( myoblasts ) are a type of stem cells that are still largely undifferentiated and are located in the vicinity of muscle fibers. (zxc.wiki)
Because of the decreased proliferation potential [9] and lower absolute number of satellite cells in muscles from the aged rats compared with adult rats [1,10,11] , one may hypothesize that the aged rats would not yield enough myoblasts to produce myooids in culture. (josam.org)
Skeletal muscle progenitors (satellite cells), murine cell line and immortalized human myoblasts are studied in vitro to identify, in a controlled environment, the pathways regulating the regenerative potential of the skeletal muscle. (units.it)
We also demonstrate that the transcriptional regulator PRDM16 (PRD1-BF1-RIZ1 homologous domain containing 16) controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. (bioseek.eu)
We previously reported that local injection of single, high-dose bFGF into the paralyzed vocal fold 1 month after RLN transection compensated for an atrophied TA muscle by inducing activation of SC proliferation and increasing the number of myoblasts in the RLN paralysis model of rats [ 12 ]. (biomedcentral.com)
Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. (zen-bio.com)
ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes. (zen-bio.com)
Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. (zen-bio.com)
Skeletal myoblasts are available from both male and female donors. (zen-bio.com)

Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells. (medecinesciences.org)
Certainly, when Ezh2 was erased in dedicated myogenic precursors (MyoD+), gene misexpression was hardly noticed (Supplemental Fig. H5G). (cancer-ecosystem.com)
Derivation of Skeletal Myogenic Precursors from Human Pluripotent Stem Cells Using Conditional Expression of PAX7. (uh.edu)
Our team has demonstrated some important differences between man and mouse, in the regulation of the proliferation of myogenic precursors. (institut-myologie.org)
We have chosen to work on human precursors, either in vitro or in vivo, in xenograft models that we have developed in successive European networks. (institut-myologie.org)
Therefore, in collaboration with W. Wright, we have developed an approach for the immortalisation of human muscle precursors that allows them to retain their ability to differentiate: this has enabled the generation of immortal cell lines derived from patient cells. (institut-myologie.org)
Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previously thought to be expressed only in the myogenic lineage. (bioseek.eu)

Transplantation of genetically corrected human iPSC-derived progenitors in mice with limb-girdle muscular dystrophy. (medecinesciences.org)
non-committed originate cells and Pax7+/Myf5+ muscle mass progenitors. (cancer-ecosystem.com)
Unlike additional research in which removal was conditionally acquired in dedicated progenitors or differentiated cells (Chen et al. (cancer-ecosystem.com)
Next, surface marker screen allowed identification of CD10 and CD24 for purification of myogenic progenitors. (nih.gov)
CD10 expression was also confirmed on human satellite cells and muscle progenitors. (nih.gov)
Wu J, Matthias N, Lo J, Ortiz-Vitali JL, Shieh AW, Wang SH, Darabi R . A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. (uh.edu)
Human ES and iPS-Derived Myogenic Progenitors Restore Dystrophin and Improve Contractility upon Transplantation in Dystrophic Mice. (uh.edu)
Assessment of the myogenic stem cell compartment following transplantation of pax3/pax7-induced embryonic stem cell-derived progenitors. (uh.edu)
Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)

Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. (medecinesciences.org)
Skoglund G, Lainé J, Darabi R , Fournier E, Perlingeiro R, Tabti N. Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. (uh.edu)
Satellite cells are in general easy to isolate from explanted skeletal muscle, and presumably muscle biopsies from an adult mammal would provide an ample supply of cells committed to the myogenic developmental pathway to facilitate the in vitro engineering of functional skeletal muscle. (josam.org)
To study the capacity of satellite cells to form into functional skeletal muscle tissue de novo in culture, we have developed several in vitro tissue models. (josam.org)
Myooids were activated by transverse electrical fields in a manner similar to that used to activate whole skeletal muscles in vitro during standard physiological measures of muscle function [8] . (josam.org)
10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)
To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
Since only small numbers of cells are obtainable from the patient, it requires expansion of cells in vitro . (sciepub.com)
Nevertheless, these primary cells grow slowly in vitro and lose their general characteristics during prolonged culturing period. (sciepub.com)
Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)

Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle. (medecinesciences.org)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
We determined the developmental and functional status of both skeletal muscle progenitor cells as well as adult human satellite cells. (ca.gov)
A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. (ca.gov)
Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)

Thereby, the increased tension per individual cross bridge and the stretching forces on sarcomeres induce microtrauma in muscle fibers [ 3 ]. (hindawi.com)
Microscopically, observations show ruptures of Z-discs and A-bands and the subsequent dissolution of sarcomere structures in individual muscle fibers [ 5 , 6 ]. (hindawi.com)
When adapting muscles through muscle building, a distinction can be made between muscle hypertrophy , the thickening of existing muscle fibers, and muscle hyperplasia , the formation of new muscle fibers, the existence of which is controversial in humans. (zxc.wiki)
Muscle fibers are the constituent parts of a muscle. (zxc.wiki)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
In agreement with this, muscles enriched in fast-glycolytic myofibers such as the extensor digitorum longus and gastrocnemius, but not those rich in oxidative fibers such as the soleus, exhibited a reduced muscle size in homozygous HIV-1 transgenic mice compared to their littermate control counterparts. (imrpress.com)
3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)
This study aimed to investigate the influence of Sustanon on the morphology of skeletal muscle fibers and the distribution of myogenic stem cells known as Satellite Cells (SCs) during postnatal growth. (colorectal-cancer.net)
Until recently, scientists thought this meant that nuclei - the cell control centers that build and maintain muscle fibers - are also lost to sloth. (robert-gorter.info)
Two independent studies - one in rodents and the other in insects - have demonstrated that nuclei are not lost from atrophying muscle fibers, and even remain after muscle death has been initiated. (robert-gorter.info)

Cell Stem Cell. (wikipedia.org)
Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche. (medecinesciences.org)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
Wu J, Hunt SD, Matthias N, Servián-Morilla E, Lo J, Jafar-Nejad H, Paradas C, Darabi R . Generation of an induced pluripotent stem cell line (CSCRMi001-A) from a patient with a new type of limb-girdle muscular dystrophy (LGMD) due to a missense mutation in POGLUT1 (Rumi). (uh.edu)
Matthias N, Hunt SD, Wu J, Darabi R . Skeletal muscle perfusion and stem cell delivery in muscle disorders using intra-femoral artery canulation in mice. (uh.edu)
Therefore, these results establish the efficacy of diprotin A in stimulating muscle cell engraftment, and highlight the pre-clinical utility of a xenotransplantation model in assessing the relative efficacy of muscle stem cell populations. (biomedcentral.com)
We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
We determined the true SC state needed to repopulate after reinjury- the gold standard requirement for maintaining stem cell function long term. (ca.gov)
The research in this propoal will benefit CA residents tremendously by understanding how to generate a muscle stem cell capable of responding to injury and undergoing long term repopulation after injury to generate new muscle. (ca.gov)
Cell Stem Cell 2013;13(5):590-601. (scielo.br)
From a research, medical, and business standpoint, mesenchymal stem cell (MSC)-based therapies are fascinating. (biomedcentral.com)

miR-133 is expressed in muscle tissue and appears to repress the expression of non-muscle genes. (wikipedia.org)
BMP2 controls bone cell determination by inducing miRNAs that target muscle genes but mainly by down-regulating multiple miRNAs that constitute an osteogenic program, thereby releasing from inhibition pathway components required for cell lineage commitment establish a mechanism for BMP morphogens to selectively induce a tissue-specific phenotype and suppress alternative lineages. (wikipedia.org)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
To achieve this we firstly established a mouse BAT enriched-miRNA profile by comparing miRNAs expressed in mouse BAT, white adipose tissue and skeletal muscle. (biomedcentral.com)
Discovery of active human brown adipose tissue (BAT) has renewed interest in the possibility that it may regulate metabolism and EE in humans [ 3 - 5 ]. (biomedcentral.com)
Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
Thereby, several studies described the occurrence of a local inflammation in muscle tissue. (hindawi.com)
Prior to tissue engineering of functional skeletal muscle from satellite cells harvested from aged mammals, two key questions must be addressed. (josam.org)
The purpose of the current study was to compare the excitability and contractility of myooids engineered from skeletal muscles of 32-month-old rats with that of myooids engineered from adult rat tissue. (josam.org)
Skeletal muscle is a target tissue for anabolic steroids. (frontiersin.org)
However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
Skeletal muscle is the largest tissue in the body. (frontiersin.org)
Skeletal muscle is a complex tissue composed of thousands of individual multinucleated myofibers. (arvojournals.org)
The response of target cells is further regulated by their microenvironment, involving the extracellular matrix, which may be modified by MSC-produced matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs. (biomedcentral.com)
MSCs can be isolated from a range of tissues, but the most commonly cited sources are bone marrow (BM), adipose tissue, muscle, bone, and perinatal tissues (e.g. (biomedcentral.com)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
She has been reviewer for international Journals ( FASEB J , Cell & Tissue Res , Toxicon, Exp Cell Res ), for national grant agencies (MIUR) and international Foundations (AFM, Association Francaise contre les Myopathies). (units.it)
With ageing, skeletal muscle undergoes a severe reduction in tissue mass, leading to a decrease in strength (sarcopenia). (units.it)
Tissue-engineering and cell-based therapies, two prominent branches of Regenerative Medicine, are considered as the most suitable techniques to repair the tissue damage. (sciepub.com)
Exosomes are membrane-bound nanovesicles with diameters of 30-150 nm that contain multiple proteins, nucleic acid, lipids and other molecules in a tissue- and cell-specific manner. (biu.ac.il)
Until quite recently, brown adipose tissue (BAT) was thought to be of metabolic importance only in smaller mammals and infant humans. (bioseek.eu)
It's actually a special type of tissue in your body, where cells are fused together extra close - so close, that they behave a like a giant single cell. (robert-gorter.info)
Additionally, each gene may have other tissue expression, which can result in non-muscle symptoms. (medscape.com)

In vivo studies using transgene/reporter-free hESC/iPSCs further validated myogenic potential of the cells by dystrophin restoration and satellite cell engraftment in NSG-mdx4cv mice. (nih.gov)

Graduates will understand how to meet the nutritional needs of exercise, training, and conditioning to enhance human health, function, and performance as well as the underlying mechanisms at the molecular level to the whole body. (csulb.edu)
The objective of this study was to elucidate the effects of early bFGF administration on the thyroarytenoid muscle after RLN transection and to investigate the underlying mechanisms. (biomedcentral.com)

At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)
We are developing a target product profile for human satellite cells derived from human pluripotent stem cells which will be used to use muscle stem cells in personalized medicine for patients with devastating muscle wasting or degenerative diseases. (ca.gov)

The mechanical metabolic pathway is activated by microtraumas and is of greater importance with regard to hypertrophy, since, in addition to an increase in the protein biosynthesis rate , it leads to a fusion of satellite cells with the muscle cell, whereby the total amount of DNA in the cell increases and thus the hypertrophy potential increases. (zxc.wiki)
This increases the total amount of DNA in the muscle cell and thus - due to new MNDs - the hypertrophy potential. (zxc.wiki)
The exact, extremely complex mechanisms of hypertrophy are not yet fully understood, but thanks to new technologies such as magnetic resonance imaging , which can take three-dimensional images of individual muscles, and dual x-ray absorptiometry , hypertrophy has been researched in more detail in recent years. (zxc.wiki)
High levels are associated with muscle hypertrophy, whereas low levels are epidemiologically associated with metabolic syndrome and diabetes, which negatively impact muscle functions. (frontiersin.org)
There is a general agreement that hypertrophy is accompanied by the addition of new nuclei from stem cells to help the muscles meet the enhanced synthetic demands of a larger cell. (frontiersin.org)
Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)
This study aimed to determine the efficacy of functional training with and without blood flow restriction (BFR) on muscle hypertrophy indices and strength in older men. (biomedcentral.com)
The findings showed that FT was effective in increasing circulating biomarkers involved in hypertrophy in older adults while adding BFR to FT had a slight increase in these biomarkers but had a tremendous increase in muscle strength. (biomedcentral.com)
Myokines are peptides or proteins released from skeletal muscle and are involved in multiple physiological processes, including metabolism and hypertrophy in the autocrine and paracrine manner [ 6 ]. (biomedcentral.com)

The regulation of SC activity is governed by myogenic regulatory factors (MRF), including MyoD, Myf5, myogenin, and MRF4. (mcmaster.ca)
2009). We produced rodents in AZD8330 manufacture which was conditionally ablated in SCs (muscle mass knockout, mouse, where cells that communicate or possess ever indicated Myf5-Cre are YFP+, while cells that possess by no means indicated Myf5-Cre are YFP? (cancer-ecosystem.com)
A even more unsuspecting and plastic material chromatin environment, such as that of Sera cells or non-committed Pax7+/Myf5? (cancer-ecosystem.com)
Wu J, Hunt SD, Xue H, Liu Y, Darabi R . Generation and Characterization of a MYF5 Reporter Human iPS Cell Line Using CRISPR/Cas9 Mediated Homologous Recombination. (uh.edu)
Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)

This work could provide a cell therapy for muscle disorders including muscular dystrophies or sarcopenia. (ca.gov)
A large majority of California residents are affected or will be affected with a muscle disease in their lifetime including severe muscular dystrophies, aging related muscle loss called sarcopenia or muscle weakness. (ca.gov)

miR-133 suppresses Prdm16 expression in skeletal muscle stem cells (satellite cells), which controls myogenic vs. brown adipogenic lineage determination in these cells. (wikipedia.org)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)

Rodents with conditional mutilation of Ezh2 in Pax7-produced muscle mass precursor cells possess decreased muscle mass mass with smaller sized myofibers was selectively ablated in Pax7-produced cells by traversing knock-in rodents articulating Cre recombinase from the Pax7 locus (alleles (Su et al. (cancer-ecosystem.com)
In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)
The addition of BrdU-positive myonuclei increased from 1 per 1000 myofibers in cross sections of control muscles to 2 to 3 per 100 myofibers in the resected muscles. (arvojournals.org)
Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
It is considered postmitotic, in that the myonuclei residing within these myofibers do not divide in adult muscle. (arvojournals.org)
When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)

NEW & NOTEWORTHY The myogenic program is controlled via transcription factors referred to as myogenic regulatory factors (MRF). (mcmaster.ca)
Of particular importance has been the identification of two families of transcription factors: the myogenic regulatory factors (MRFs) and the myocyte enhancer factors (MEFs). (biologists.com)
The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)

Dystrophin expression in muscles of Duchenne muscular dystrophy patients after high-density injections of normal myogenic cells. (medecinesciences.org)
Duchenne muscular dystrophy (DMD), the most common and severe form of muscular dystrophy, is caused by mutations in the dystrophin gene, the largest gene identified in the human genome. (biomedcentral.com)
Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)

Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)

Current research scope includes: central vs. local hormonal control of muscle growth and nutrient metabolism, acute nutritional state and its impact on exercise-induced glycemic control, understanding how nutritional aids classically used to improve sport performance can also help treat metabolic syndrome. (csulb.edu)
The size of an MND has a maximum that results from the ratio of sarcoplasm and cell nucleus, which is determined in a muscle cell by the requirements of an optimal metabolism. (zxc.wiki)
Cell Metabolism 2013;17(2):210-24. (scielo.br)
Mapping human microbiome drug metabolism by gut bacteria share metabolic pathways for anti-cancer drug metabolism. (anonymityblaize.com)
Medium pH modulates matrix, mineral, and energy metabolism in cultured chick bones and osteoblast-like cells. (sciepub.com)
BAT may thus play a much more important role in human metabolism than was previously appreciated. (bioseek.eu)

Altered activation of PI3K and SREBP-1c may explain the defective regulation of miR-1 and miR-133a expression in response to insulin in muscle of type 2 diabetic patients. (wikipedia.org)
Recent mechanistic insights into the trophic activities of these cells point to ultimate regulation by nitric oxide, nuclear factor-kB, and indoleamine, among other signaling pathways. (biomedcentral.com)
Yamaguchi DT, Huang JT, Ma D. "Regulation of gap junction intracellular communication by pH in MC3T3-E1 osteoblastic cells. (sciepub.com)

All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)
Understanding the effects of strabismus surgery on muscle cell biological reactions and myofiber remodeling may suggest new approaches for improving surgical outcomes. (arvojournals.org)
There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)

The large number of miRNAs found only in mouse BAT or only in human BAT highlights the differing molecular profile between species that is likely to influence the functional role of BAT across species. (biomedcentral.com)
Functional three-dimensional skeletal muscle constructs (myooids) were engineered from myogenic cells harvested from the muscles of adult and aged rats. (josam.org)
First, to what extent are the satellite cells from aged mammals capable of fusion into functional myotubes, and second, do satellite cells from aged mammals have any form of impaired function when compared with those from young or adult mammals? (josam.org)
Evaluate myogenic ability and functional differences of embryonic, fetal, juvenile and adult MuSCs in fusion, proliferation, engraftment and repopulation assays in mouse models of muscle disease. (ca.gov)
These changes include elongation, polarization, aggregation and fusion, which ultimately give rise to functional muscle. (biologists.com)
Although administration of various myogenic cell types has been done intramuscularly or intravascularly, functional restoration has not been achieved yet in patients with degenerative diseases affecting multiple large muscle. (crick.ac.uk)
Several types of adenosine receptors have been detected in developing and differentiated skeletal muscle cells, but little is known about the functional outcome of AR modulation on muscle AChRs. (units.it)
We reported that LIPUS therapy at intensity of 60mW/cm 2 exhibited the most significant differences in functional recovery of contusion-injured muscle in mice. (ijbs.com)
The comprehensive functional tests and histological analysis in vivo indirectly and directly proved the effectiveness of LIPUS for muscle recovery. (ijbs.com)

With MyoD being a protein that is involved in the very first step of satellite cell recruitment (it stops the proliferation of stems cells and initiates their transformation to muscle cells) this may seem awkward at first. (blogspot.com)
In view of the MyoD promoting effects of trichostatin A , which basically stops cell development in its tracks it can however be explained by the fact that less new satellite cells are needed, because their survival is increased so that the proliferation rate does not actually suffer ( figure 1 , left). (blogspot.com)

Kurt Escobar, PhD, CISSN - The evolutionary aspects of the effects of exercise and nutrition on human health, aging, and performance including applied physiology and the underlying cellular mechanisms. (csulb.edu)
The main interest of her research activity is the nerve-muscle communication and the skeletal muscle physiology, including muscle plasticity and impact of ageing on the neuromuscular system. (units.it)
The results of the proposed research will advance the understanding of important aspects of the neuromuscular physiology such as the modulation of the neuromuscular transmission and the skeletal muscle plasticity. (units.it)
But according to a review published in Frontiers in Physiology, modern lab techniques now allow us to see that nuclei gained during training persist even when muscle cells shrink due to disuse or start to break down. (robert-gorter.info)
It is well documented in the field of exercise physiology that it is far easier to reacquire a certain level of muscle fitness through exercise than it was to achieve it the first place, even if there has been a long intervening period of detraining. (robert-gorter.info)

Skeletal muscle myosin heavy chain (MHC) content was measured and MHC isoforms were separated on SDS-PAGE gels and quantified. (josam.org)
Adult and aged rat myooids had only ~35-60% of the skeletal muscle MHC content of control skeletal muscle from rats, the remaining MHC content consisting of isoforms found in cultured fibroblasts but not in control skeletal muscle. (josam.org)
In addition, myooids expressed only developmental isoforms of skeletal muscle MHC, known to generate less specific force than adult isoforms. (josam.org)
In this project, the group intends to investigate the nature and the role of adenosine and AR signalling pathways on the two isoforms of muscle AChRs: the embryonic (g-AChR) regulating synaptogenesis and muscle development and the adult (e-AChR) with a role in nerve-muscle communication. (units.it)

mutilation in Pax7-produced skeletal muscle mass AZD8330 manufacture cells outcomes in problems of postnatal muscle mass development characterized by decreased muscle mass mass with smaller sized muscle mass materials. (cancer-ecosystem.com)
Conditional Ezh2 mutilation outcomes in postnatal skeletal muscle mass problems and an impoverished South carolina pool. (cancer-ecosystem.com)
Among the many different therapeutic approaches, including exercise, hormone, nutritional therapy, and/or a combination thereof, no one approach has emerged as superior in animal or human studies, and the great variability in the clinical outcomes of patients treated with these approaches has prevented identification of the most effective therapy ( 7 , 8 ). (frontiersin.org)

Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells. (medecinesciences.org)

This strategy allowed pathway screen to define efficient myogenic induction in hESC/iPSCs. (nih.gov)
Cell survival responses to environmental stresses via the Keap1-Nrf2-ARE pathway. (jeoct.com)

There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
The epidemic of obesity, closely associated with increases in diabetes, hypertension, hyperlipidemia, cancer and other disorders, has propelled a major interest in adipose cells and tissues. (bioseek.eu)
Adipose tissues contain two distinct types of fat cells, white and brown. (bioseek.eu)

The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)

Aldehyde dehydrogenase activity identifies a population of human skeletal muscle cells with high myogenic capacities. (medecinesciences.org)

His studies were focused on the identification of different exercise and nutritional intervention strategies to further augment skeletal muscle protein synthesis and muscle satellite cell function in older adults. (maastrichtuniversity.nl)
Here are focal adhesion kinase (FAK) is activated, the set a chain of reactions in the sequence which the synthesis of muscle protein (e.g., contractile proteins such as actin and myosin , cytoskeletal proteins and enzymes of the citric acid cycle stimulate). (zxc.wiki)
The problem with building muscle is that, due to the synthesis of muscle protein, cytoskeletal proteins (supporting proteins) are also synthesized, which are supposed to protect the cell from mechanical overload. (zxc.wiki)

PRDM16 controls a brown fat/skeletal muscle switch. (bioseek.eu)
Finally, Prdm16-deficient brown fat displays an abnormal morphology, reduced thermogenic gene expression and elevated expression of muscle-specific genes. (bioseek.eu)

Here we show that, concurrent with a decrease in chondrogenesis, inhibition of p38 in limb bud cultures dramatically promotes muscle development. (biologists.com)
Increase inflammation promotes apoptosis in the epithelial cells, endothelial cells, and airways, that resulting Emphysema. (jeoct.com)

miR-133 enhances myoblast proliferation by repressing serum response factor (SRF) mIR-133 suppresses SP1 expression In rats, miR-133b is expressed in retinal dopaminergicamacrine cell, and this expression is significantly increased during early stage during retinal degeneration. (wikipedia.org)
Body and muscle mass mass had been significantly decreased in mutilation in skeletal myogenic cells. (cancer-ecosystem.com)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
The cross-sectional thyroarytenoid muscle area was significantly larger in the high-dose group than in the saline and low-dose groups on days 28 and 56. (biomedcentral.com)
Immunohistochemistry indicated that bFGF significantly increased the number of satellite cells in the thyroarytenoid muscle up to day 14 and that of neuromuscular junctions on days 28 and 56. (biomedcentral.com)

A perivascular origin for mesenchymal stem cells in multiple human organs. (medecinesciences.org)
Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. (biomedcentral.com)

Most of the known, partly still popular, theories, such as the hypothesis of increased blood circulation, the muscle hypoxia hypothesis and the ATP deficiency hypothesis (the ATP level in the cell remains constant), have been refuted. (zxc.wiki)
These and other data argue against the current interpretation of the myonuclear domain hypothesis and suggest that once a nucleus has been acquired by a muscle fiber it persists. (frontiersin.org)

Specifically,treatment of primary limb bud cultures with p38 inhibitors increases the expression of myogenic markers and causes a striking increase in formation of myotubes, which were detected using antibodies specific for myosin heavy chain. (biologists.com)

In conclusion, our results suggest that the directional changes in mRNA expression of the MRF in a whole muscle homogenate in response to acute eccentric exercise reflects that observed in isolated muscle SC. (mcmaster.ca)
Evan Schick, PhD, CSCS - Central research interest surrounds the medicinal aspects of exercise and nutrition as well as how skeletal muscle health influences total body health and sport performance. (csulb.edu)
As an assistant Professor at Maastricht University, he is now involved in both research and education focused on the skeletal muscle fiber perfusion in muscle adaptive response to exercise training in young, old and more compromised clinical populations. (maastrichtuniversity.nl)
4mg/kg ~ 300-400mg for an average adult) it exerted significant ameliorative effects on exercise induced muscle damage (Malaguti. (blogspot.com)
The results of the current study indicate that Tr14 seems to limit exercise-induced muscle damage most likely via attenuation of both innate and adaptive immune responses. (hindawi.com)
Eccentric exercise like downhill running is a special exercise mode which is known to cause substantial muscle damage followed by a pronounced inflammatory response. (hindawi.com)
This muscle damage is accompanied by muscle soreness, which regularly occurs 12-48 hours after the eccentric exercise bout and which was termed delayed onset of muscle soreness (DOMS). (hindawi.com)
Exercise training and muscle-lung crosstalk: The emerging roles of Irisin and Semaphorin-3A in pulmonary diseases. (jeoct.com)
Exercise can produce Irisin and Semaphorin-3A by stimulating muscle and nerve cell, which have positive effects on other tissues, including the lungs. (jeoct.com)
Therefore, interventions such as exercise training that mitigate muscle loss may be associated with changes in the circulatory level of irisin and F:M ratio. (biomedcentral.com)
It is often used to improve exercise performance and muscle mass. (medlineplus.gov)
People commonly use creatine for improving exercise performance and increasing muscle mass. (medlineplus.gov)
It seems to work best when used along with exercise to build muscles. (medlineplus.gov)

Dual luciferase assay and Western-blot results demonstrated that SFRP1 was a target of miR-1/206 in porcine iliac endothelial cells. (biomedcentral.com)
1999). such as platelets, macrophages, fibroblasts, epithelial The effect of anabolic steroids appears due to an cells, and endothelial cells. (sagepub.com)

Autologous transplantation of muscle-derived CD133 + stem cells in Duchenne muscle patients. (medecinesciences.org)
Additionally, safe and efficient migration of transplanted cells is critical in cell therapies, both allogeneic and autologous. (crick.ac.uk)
However, a huge number of autologous cells are required to address these practices. (sciepub.com)

The present double-blind, randomized, placebo-controlled clinical trial intended to test whether ingestion of a natural combination medicine (Tr14 tablets) affects serum muscle damage and inflammatory immune response after downhill running. (hindawi.com)
Background Inflammatory myopathies (IM) are a heterogeneous group of disorders characterized by autoimmune inflammatory destruction of skeletal muscles. (biu.ac.il)
But modern cell-type-specific dyes and genetic markers have shown that the dying nuclei other researchers had detected were in fact inflammatory and other cells recruited to atrophic muscle. (robert-gorter.info)

Together, these data suggest that CXCR4 plays an important role in donor cell engraftment. (biomedcentral.com)
Therefore, we sought to translate these results to a clinically relevant canine-to-canine allogeneic transplant model for Duchenne muscular dystrophy (DMD) and determine if CXCR4 is important for donor cell engraftment. (biomedcentral.com)
We show that CXCR4 expressing cells are important for donor muscle cell engraftment, yet FACS sorted CXCR4-positive cells display decreased engraftment efficiency. (biomedcentral.com)
We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)
One of the key reasons for this negative outcome is the limited migration of donor cells, which hinders the overall cell engraftment potential. (crick.ac.uk)

Comparing and contrasting the molecular mechanisms controlling BAT growth and development in mice and humans will increase our understanding or how human BAT is developed and may identify potential therapeutic targets to increase EE. (biomedcentral.com)
MicroRNAs are molecular mechanisms involved in mouse BAT development however, little is known about the miRNA profile in human BAT. (biomedcentral.com)
Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
Nature Reviews Molecular Cell Biology 2012;13(2):127-33. (scielo.br)
Molecular biology of the cell. (kcl.ac.uk)
Additionally, our data in progress suggest that muscle and brain age by similar molecular mechanisms and thus, therapeutic strategies for rejuvenating muscle repair might be applicable to the restoration of neurogenesis in aged brain. (ca.gov)

RhoA expression is negatively regulated by miR-133a in bronchial smooth muscles (BSM)and miR-133a downregulation causes an upregulation of RhoA, resulting in an augmentation of contraction and BSM hyperresponsiveness. (wikipedia.org)
The present study showed that in addition to Irisin, semaphorin-3A induced by muscle contraction, prevents inflammation and lung damage caused by COPD or other lung disease. (jeoct.com)

Androgens are the main physiologic anabolic steroid hormones and normal testosterone levels are necessary for a range of developmental and biological processes, including maintenance of muscle mass. (frontiersin.org)

Immune cells that infiltrate lesions are important for atherosclerosis progression and immunotherapies. (biomedcentral.com)

Sarcopenia, the age-related loss of skeletal muscle mass and function, is becoming more prevalent as the lifespan continues to increase in most populations. (frontiersin.org)

The aims of this study were to establish a mouse BAT-enriched miRNA profile and compare this with miRNAs measured in human BAT. (biomedcentral.com)
We also identified 145 miRNAs expressed in both mouse and human BAT, of which 25 were enriched in mouse BAT. (biomedcentral.com)
For the first time, 69 miRNAs were identified in human BAT but absent in mouse BAT, and 181 miRNAs were expressed in mouse but not in human BAT. (biomedcentral.com)
The present study has identified a small sub-set of miRNAs common to both mouse and human BAT. (biomedcentral.com)
Moreover, miRNAs play a modulatory role in the development and growth of skeletal muscles [ 22 ]. (biomedcentral.com)
Three miRNAs, miRNA-1, −133 and −206, are specifically expressed in muscle and are considered to be myomiRs [ 23 , 24 ]. (biomedcentral.com)
It's been suggested that miRNAs regulate up to 50% of all mRNAs in the human genome. (genengnews.com)

The mRNA expression of these MRF in humans following muscle damage has been predominately measured in whole muscle homogenates. (mcmaster.ca)
Whether the temporal expression of MRF in a whole muscle homogenate reflects SC-specific expression of MRF remains largely unknown. (mcmaster.ca)
Previous studies have derived MRF expression from whole muscle homogenates, but little work has examined whether the mRNA expression of these transcripts reflects the pattern of expression in the actual population of satellite cells (SC). (mcmaster.ca)
We report that MRF expression from an enriched SC population reflects the directional pattern of expression from skeletal muscle biopsy samples following eccentric contractions. (mcmaster.ca)
The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)
Mice develop tumors resembling those seen in humans, including expression of high levels of miR-21. (genengnews.com)
Each cell nucleus has a certain "sphere of influence" which it controls through its gene expression. (zxc.wiki)
Normalizing for skeletal muscle MHC content and isoform expression predicts myooid forces of ~6-18% of control adult skeletal muscles. (josam.org)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)

The homozygous HIV-1 transgenic mouse line Tg26 reproduces the wasting syndrome of AIDS patients, thus constituting a valid animal model to characterize the muscle phenotype induced by HIV infection. (imrpress.com)

We hypothesize that the remaining force deficit is due to cellular and sub-cellular disorganization-Myooids lack the density and organization of sarcomeric arrays seen in skeletal muscle. (josam.org)
Studies targeted at establishing such effects at cellular level and their correlations with in vivo models, will broaden our understanding of the roles played by androgens on skeletal muscle function in elderly. (frontiersin.org)
We have a strong expertise on human cellular models (this includes the immortalization facility that we have initiated) - and xenotransplantation (this includes several immunodeficient mouse models and grafting procedures). (institut-myologie.org)

Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)
Since no myogenic intrinsic defect was observed in satellite cells from the transgenic mice, these results support the notion of an inflammation-mediated, fiber-type-specific inhibition of muscle growth in the presence of the HIV-1 transgene. (imrpress.com)
Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)

The decreased quantity of Pax7+ cells in = 2) 3 m after CTX, probed with myogenin, Pax7, and GAPDH antibodies. (cancer-ecosystem.com)
Wu J, Hunt SD, Xue H, Liu Y, Darabi R . Generation and Validation of PAX7 Reporter Lines from Human iPS Cells Using CRISPR/Cas9 Technology. (uh.edu)
We will identify unique cell surface markers on in vivo derived MuSCs that distinguish PAX7-positive cells from other muscle cells and across cell states. (ca.gov)

Findings The present research exposed that EZH2 affects many elements of South carolina biology, including self-renewal, expansion, and cell identification. (cancer-ecosystem.com)
Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development. (biomedcentral.com)

In 2010 beginning of 2011, we have approached the identification and characterization of the proteins that are produced by hESCs and have the rejuvenating and pro-regenerative activity on adult muscle. (ca.gov)

These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)

Anatomy16

Organisms3

Diseases2

Chemicals and Drugs14

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes14

Anthropology, Education, Sociology and Social Phenomena1

Information Science3

Differentiation23

Regeneration31

Myogenesis7

Atrophy13

Myoblasts12

Precursors7

Progenitors9

Vitro10

Progenitor cells5

Fibers10

Stem Cell12

Tissue26

Validated myogenic potential1

Underlying mechanisms2

Pluripotent2

Hypertrophy9

Myf55

Muscular dystrophies2

Lineage3

Myofibers7

Regulatory3

Dystrophin3

Primary skeletal1

Metabolism6

Regulation3

Myofiber3

Functional9

MyoD2

Physiology5

Isoforms4

Outcomes3

Precursor cells1

Pathway2

Adipose4

Postnatal1

Identifies1

Protein3

Prdm162

Promotes2

Significantly5

Mesenchymal2

Hypothesis2

Myotubes1

Exercise13

Endothelial cells2

Autologous3

Inflammatory3

Donor5

Molecular6

Contraction2

Developmental1

Progression1

Populations1

MiRNAs7

Expression9

Phenotype1

Cellular3

Mice3

PAX73

Affects2

Adult muscle1

Physiological1