• Gene
  • At the molecular level, this involves application of a novel strategy for probing the MyoD regulatory network (forced dimer polyproteins), and further development and application of in vivo footprinting to help understand how protein:DNA complex formation is regulated and related to gene activity in the cell. (searlescholars.net)
  • MyoD family inhibitor is a protein that in humans is encoded by the MDFI gene. (wikipedia.org)
  • This is caused, in part, by an accelerated induction of MyoD, myocyte enhancer-binding factor 2 (MEF2), p21Cip1, and p27Kip1, a faster down-regulation of Id1, and an increase in MEF2-dependent gene transcription. (rupress.org)
  • Additionally, 9E11G cells expressed transcripts for MHox , a muscle homeobox gene expressed in smooth, cardiac, and skeletal muscles, but not the skeletal muscle-specific regulatory factors, MyoD and myogenin. (ahajournals.org)
  • Although MyoD marks myoblast commitment, muscle development is not dramatically ablated in mouse mutants lacking the MyoD gene. (wikipedia.org)
  • MyoD is known to have binding interactions with hundreds of muscular gene promoters and to permit myoblast proliferation. (wikipedia.org)
  • MyoD has also been shown to function cooperatively with the tumor suppressor gene, Retinoblastoma (pRb) to cause cell cycle arrest in the terminally differentiated myoblasts. (wikipedia.org)
  • Myogenin (myogenic factor 4), also known as MYOG, is a gene. (wikipedia.org)
  • These include, but are not limited to: CACGTT sequence 20 bp upstream of the mouse Period2 (PER2) gene and regulates its expression CAGCTT sequence found within the MyoD core enhancer CACCTCGTGAC sequence in the proximal promoter region of human and rat APOE, which is a protein component of lipoproteins. (wikipedia.org)
  • Perhaps the work for which Weintraub is best known was his laboratory's discovery and characterization of "myoD", the first master regulatory gene. (wikipedia.org)
  • mef2c
  • The underlying molecular mechanism lies in the ability of IFRD1 to cooperate with MyoD at inducing the transcriptional activity of MEF2C. (wikipedia.org)
  • protein
  • The researchers expressed the complementary DNA (cDNA) of the murine MyoD protein in a different cell lines (fibroblast and adipoblast) and found MyoD converted them to myogenic cells. (wikipedia.org)
  • Later studies by the same group of investigators at FHCRC further characterized the structural and functional characteristics of myoD and its nuclear-localized protein product, which were found to be present in organisms as diverse as nematode worms, frogs, mice, and humans. (wikipedia.org)
  • myoblasts
  • In response to muscle injury or exercise, these quiescent MSCs become activated, as indicated by the expression of MyoD, reenter the cell cycle, and actively proliferate to form myoblasts. (rupress.org)
  • inhibitor
  • The activity of MyoD and related family members is subject to regulation by changes in phosphorylation and expression of an inhibitor of DNA binding, Id. (ahajournals.org)
  • In one model of Setdb1 action on MyoD, Setdb1 represses an inhibitor of MyoD. (wikipedia.org)
  • This unidentified inhibitor likely acts competitively against MyoD during typical cellular proliferation. (wikipedia.org)
  • functional
  • MyoD was cloned by an ingenious functional assay for muscle formation reported in Cell in 1987 by Davis, Weintraub, and Lassar. (wikipedia.org)
  • While this demonstrates that MYF-6 might not be essential for the formation of myofibers, it is thought that myogenin compensates for the absence of functional MYF-6. (wikipedia.org)
  • quiescent
  • In contrast, MyoD is not expressed in quiescent MSCs. (rupress.org)
  • MyoD is expressed at extremely low and essentially undetectable levels in quiescent satellite cells, but expression of MyoD is activated in response to exercise or muscle tissue damage. (wikipedia.org)
  • transcriptional
  • More recently it has been shown that IFRD1 potentiates muscle regeneration by a second mechanism that potentiates MyoD, i.e., by repressing the transcriptional activity of NF-κB, which is known to inhibit MyoD mRNA accumulation. (wikipedia.org)
  • expression
  • Once the "master controller" MyoD has become active, SETDB1 is required to maintain MyoD expression within the cell. (wikipedia.org)
  • PAX3 mediates the transcription of c-Met and is responsible for the activation of MyoD expression-one of the functions of MyoD is to promote the regenerative ability of satellite cells (described below). (wikipedia.org)
  • known
  • MyoD has since been an active area of research as still relatively little is known concerning many aspects of its function. (wikipedia.org)
  • positive
  • Therefore, IFRD1 appears to act as a positive cofactor of MyoD. (wikipedia.org)
  • Immunostaining for myogenin and for MyoD can be used to determine ARMS from other rhabdomyosarcoma tumors and immunostaining for AP2β and p-cadherin can distinguish fusion positive ARMS from fusion negative. (wikipedia.org)
  • function
  • Given their extraordinary power to drive or even redirect a cell fate decision, one experimental goal is to understand how MyoD family regulators function. (searlescholars.net)