Myoblasts. Medical search. Frequent questions

Moreover, YK-11 has anabolic activity in vitro in C2C12 myoblasts and shows greater potency than dihydrotestosterone (DHT) in this regard. (theeagleelite.com)
Neither siRNA-mediated Stac3 knockdown nor plasmid-mediated Stac3 overexpression affected the proliferation of C2C12 myoblasts. (vt.edu)
Stac3 knockdown promoted the differentiation of C2C12 myoblasts into myotubes as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA and protein expression of myogenic markers including myogenin and myosin heavy chain. (vt.edu)
Pretreatment of murine myoblast (C2C12) cells with octyl-D-carnosine or carnosine enhanced HIF-1α protein expression, VEGF mRNA levels and VEGF release under hypoxic conditions. (frontiersin.org)
cell separations reported here relied on the different cell types (C2C12 myoblasts, myotubes and MRC-5 fibroblasts) exhibiting different the effective permittivity values. (technuc.com)
On the basis of the effective DEP-based cell separations reported right here, the same conclusion might be reached regarding splendour between stages of C2C12 myoblast cell difference. (technuc.com)
Cell civilizations (1) C2C12 mouse myoblast cell range: Cells had been plated at a thickness of 3000 cells/cm2 and expanded in high blood sugar Dulbecco's customized Eagle moderate (DMEM) development moderate (General motors) supplemented with 10% fetal bovine serum (FBS) (HyClone), 2?millimeter of 4-HQN IC50 L-glutamine, 100 products/ml of penicillin and 100?beliefs. (technuc.com)
t) Superimposed forwards spread plots of land … The forwards scatter plots of land attained for C2C12 myoblasts (n? (technuc.com)
In this study, we investigated whether or not melatonin inhibits apoptotic and autophagic cell death in C2C12 murine myoblast cells. (elsevierpure.com)
Together, these results suggest that melatonin protects against apoptotic and autophagic cell death through the common pathway resulted in the increment of Bcl-2 expression and the reduction of Bax expression in C2C12 murine myoblast cells. (elsevierpure.com)
The IGF-1 gene was cloned into the lentiviral shuttle plasmid pCDH-cGFP and the recombinant lentiviral vector was transducted into myoblast C2C12 cell line. (cellmolbiol.org)
The results of our study suggests that constructed recombinant lentiviral vector can potentially be used for regulating the expression of IGF-1 in myoblast C2C12 cells. (cellmolbiol.org)

Compared to wild-type myoblasts, myoblasts from Stac3 knockout mouse embryos showed accelerated differentiation into myotubes in culture as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA expression of myogenic markers. (vt.edu)
Moreover, although myoblasts from patients affected with FSHD fully differentiated into multinucleated myotubes, they fused to form either thin and branched myotubes with aligned nuclei or large myotubes with random nuclei distribution. (fshfriends.org)
Myoblast fusion into functionally-distinct myotubes to form in vitro skeletal muscle constructs under differentiation serum-free conditions still remains a challenge. (elsevierpure.com)
a mouse myoblast cell line) into robust myotubes was found only on GlcNAc6 micropatterns, whereas the myoblasts on control, non-patterned GlcNAc6 substrates or GlcNAc6-free patterns exhibited an undifferentiated form. (elsevierpure.com)
Myoblasts were isolated from the muscle biopsies and differentiated to myotubes ex vivo. (lu.se)
TNC RNA was quantified in the biopsies, myotubes and myoblasts using RNA sequencing. (lu.se)
TNC RNA expression is higher in myoblasts and myotubes compared to skeletal muscle tissue. (lu.se)

The purpose of this study is to investigate the molecular effects of an in vitro SKM myoblast aging protocol in combination with a β-2 adrenergic receptor agonist treatment on the expression of genes related to mitochondrial health. (wku.edu)
An in-vitro model of human bone marrow mesenchymal stem cells (hBM-MSCs) myogenic commitment by synergic effect of a differentiation media coupled with human primary skeletal myoblasts (hSkMs) co-culture was developed adopting both conventional static co-seeding and perfused culture systems. (unisa.it)
This study is aimed at determining the effects of C. vulgaris on promoting muscle regeneration by evaluating myoblast regenerative capacity in vitro. (iium.edu.my)
However, the insufficient stemness of bovine myoblasts cultivated in vitro declined the ability of cell expansion and myogenic differentiation, which limited the production of cultured meat . (bvsalud.org)
Therefore, in this study, we introduced proanthocyanidins (PC, natural polyphenolic compounds) and dialdehyde chitosan (DAC, natural polysaccharides ) to explore the effects of proliferation and differentiation of bovine myoblasts in vitro . (bvsalud.org)

Primary mouse myoblast purification, characterization, and transplantation for cell-mediated gene therapy. (rupress.org)
The transplantation of cultured myoblasts into mature skeletal muscle is the basis for a new therapeutic approach to muscle and non-muscle diseases: myoblast-mediated gene therapy. (rupress.org)
The success of myoblast transplantation for correction of intrinsic muscle defects depends on the fusion of implanted cells with host myofibers. (rupress.org)
The fate of the pure myoblast populations after transplantation was monitored by labeling the cells with the marker enzyme beta-galactosidase (beta-gal) using retroviral mediated gene transfer. (rupress.org)
Within five days of transplantation into muscle of mature mice, primary myoblasts had fused with host muscle cells to form hybrid myofibers. (rupress.org)
Even without immune suppression, the hybrid fibers persisted with continued beta-gal expression up to six months after myoblast transplantation in syngeneic hosts. (rupress.org)
The properties of these cells after transplantation--the stability of resulting hybrid myofibers without immune suppression, the persistence of transgene expression, and the lack of tumorigenicity--suggest that studies of cell-mediated gene therapy using primary myoblasts can now be broadly applied to mouse models of human muscle and non-muscle diseases. (rupress.org)
Myoblasts are muscle tissue derived mesenchymal control cell progenitors that have got great potential for make use of in regenerative medication, for cardiomyogenesis grafts and intracardiac cell transplantation especially. (technuc.com)
Scholars@Duke publication: Regenerating functional myocardium: improved performance after skeletal myoblast transplantation. (duke.edu)
The ability to regenerate functioning muscle after autologous myoblast transplantation could have a important effect on patients after acute myocardial infarction. (duke.edu)

Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. (zen-bio.com)
To examine the immunobiology of primary myoblasts, we compared transplanted cells in syngeneic and allogeneic hosts. (rupress.org)
However, other cells such as myoblasts are being currently studied and showing high interest. (ehu.eus)
Scientists identified miRNA that were up-regulated in satellite cell-derived myoblasts treated with stromal derived factor-1 (SDF-1) and/or down-regulated in cells in which the expression of CXCR4 or CXCR7, that is, SDF-1 receptors, was silenced. (stemcellsciencenews.com)
Human skeletal myoblast cells were cultured and underwent serial passaging into young and senescent phases and were then treated with C. vulgaris, followed by the induction of differentiation. (iium.edu.my)
The results obtained showed that senescent myoblasts exhibited an enlarged and flattened morphology, with increased SA-β-gal expression, reduced myogenic differentiation, decreased expression of myogenin, and an increased percentage of cells in the G0/G1 phase. (iium.edu.my)
These results indicate that differentiated chick muscle cells provide some factor that induces L6 myoblasts to synthesize rat myosin light chains. (elsevierpure.com)
The expression of sarcomeric muscle-specific contractile protein genes in BC3H1 cells: BC3H1 cells resemble skeletal myoblasts that are defective for commitment to terminal differentiation. (silverchair.com)
Myogenic regeneration occurs through a chain of events beginning with the output of satellite cells from quiescent state, formation of competent myoblasts and later fusion and differentiation into myofibres. (gla.ac.uk)
PLF was located in satellite cells and/ or myoblasts, which increased in number with continued task performance, supporting our hypothesis that PLF plays a role in muscle repair or regeneration. (cdc.gov)
Periostin, on the other hand, was not present in satellite cells and/ or myoblasts. (cdc.gov)
With higher expression in undifferentiated myoblast cells than muscle tissue, it is likely that TNC plays a role in muscle tissue remodelling in humans. (lu.se)

Both enriched and clonal populations of primary myoblasts were characterized in assays of cell proliferation and differentiation. (rupress.org)
In this study we determined the potential role of Stac3 in myoblast proliferation and differentiation, two important steps of muscle development. (vt.edu)
The ability of C. vulgaris to promote myoblast differentiation was analysed through cellular morphology, real-time monitoring, cell proliferation, senescence-associated β-galactosidase (SA-β-gal) expression, myogenic differentiation, myogenin expression, and cell cycle profiling. (iium.edu.my)
We show that zinc promotes myoblast proliferation, differentiation and maturation of myofibres. (gla.ac.uk)
Effects of proanthocyanidins and dialdehyde chitosan on the proliferation and differentiation of bovine myoblast for cultured meat production. (bvsalud.org)
It is concluded that both PC and DAC promote the proliferation and differentiation of bovine myoblasts , contributing to the development of cultured meat production systems. (bvsalud.org)

however, dfdl3 myoblasts did not achieve terminal differentiation. (bham.ac.uk)

Wright, WE 1981, ' Synthesis of rat myosin light chains in heterokaryons formed between undifferentiated rat myoblasts and chick skeletal myocytes ', Journal of Cell Biology , vol. 91, no. 1, pp. 11-16. (elsevierpure.com)

To assess tumorigenicity, primary myoblasts and myoblasts from the C2 myogenic cell line were transplanted into immunodeficient mice. (rupress.org)
BMPs are known to inhibit myogenic differentiation and in a previous study we found an increased expression of a BMP family member BMP4 in DMD myoblasts. (nih.gov)
Rationale: Skeletal myoblasts (SMs) with inherent myogenic properties are better candidates for reprogramming to pluripotency. (elsevierpure.com)

FORM appears to stabilize mitochondrial biogenesis-related genes in AGED myoblasts, indicating improved overall mitochondrial health. (wku.edu)
Depletion of RBFOX2 adversely affects mitochondrial health in myoblasts, correlating with disrupted APA of mitochondrial gene Slc25a4. (utmb.edu)
In sum, our results unveil a role for RBFOX2 in fine-tuning expression of mitochondrial and contractile genes via APA in myoblasts relevant to heart diseases. (utmb.edu)

Myoblast city (Mbc) is the Drosophila melanogaster ortholog of the mammalian protein Dock180. (wikipedia.org)
Myoblast fusion in Drosophila. (stowers.org)

CYTOO has developed a first-in-class myotube model by fully maturing human primary myoblasts. (cytoo.com)
Our findings indicated that GlcNAc6-mediated integrin interactions are responsible for guiding myoblast fusion forward along with myotube formation. (elsevierpure.com)

The Myoblast city locus was identified by deletion mapping, using this technique researchers were able to isolate the location of the gene on the right arm of the third chromosome. (wikipedia.org)

Additionally, mutant myoblasts show increased autophagy when cultured in the absence of nutrients, as well as defective cell fusion and increased apoptosis. (escholarship.org)
Herein, we report that our microtopographical carbohydrate substrates composed of bioactive hexa-N-acetyl-D-glucosamine (GlcNAc6) modulated the efficiency of myoblast fusion without requiring horse serum or any differentiation medium during cell culture. (elsevierpure.com)

The aim of the current study was therefore to investigate whether inhibition of BMP signaling could be beneficial for myoblast differentiation and muscle regeneration processes in a DMD context. (nih.gov)
In conclusion, C. vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes myoblast differentiation, indicating its potential to promote muscle regeneration. (iium.edu.my)

Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. (zen-bio.com)

myofibers formed from prematurely differentiated myoblasts are dysfunctional. (vt.edu)

Lysosomal membrane proteins Lamp1 and Lamp2 show increased molecular weights in patients' myoblasts due to differential N-glycosylation. (escholarship.org)

To understand the pathological mechanisms underlying the myopathy in IBMPFD, we have studied the cellular consequences of VCP mutations in human primary myoblasts. (escholarship.org)

Occipital somites give rise to myoblasts, which form the intrinsic tongue musculature. (medscape.com)

Control heterokaryons formed between undifferentiated rat myoblasts and chick fibroblasts also failed to synthesize myosin light chains. (elsevierpure.com)

Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. (zen-bio.com)

It was established that Pax7 localises in the cytoplasm of dystrophindeficient myoblasts and high expression is retained during differentiation. (bham.ac.uk)
However, the main reason was the viral-induced inhibition of the expression of a major gap junction component, Cx43 (Connexin 43), in the transformed myoblasts. (canada.ca)

In the present study, the protective effects of EGCG with zinc were assessed on cultures of rat cardiac myoblasts exposed to hypoxia/reoxygenation (H/R) injury. (spandidos-publications.com)

We propose that Kir2.1 channels are already present at the membrane of proliferating, undifferentiated human myoblasts but in a silent state, and that Kir2.1 tyrosine 242 dephosphorylation triggers differentiation. (unige.ch)

ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes. (zen-bio.com)
To overcome these difficulties, we developed novel culture conditions that permit the purification of mouse myoblasts from primary cultures. (rupress.org)
Primary myoblasts were dependent on added bFGF for growth and retained the ability to differentiate even after 30 population doublings. (rupress.org)
The ease of isolation, growth, and transfection of primary mouse myoblasts under the conditions described here expand the opportunities to study muscle cell growth and differentiation using myoblasts from normal as well as mutant strains of mice. (rupress.org)
However, dorsomorphin repressed both BMP and TGFβ signaling and was found to be toxic to primary myoblast cell cultures. (nih.gov)

To attempt to prevent this progression, we transplanted skeletal myoblasts into cryoinfarcted myocardium of the same rabbits (autologous transfer), monitored cardiac function in vivo for two to six weeks and examined serial sections of the hearts by light and electron microscopy. (duke.edu)

The round nucleoli of chick embryo myoblasts, when grown in a culture medium devoid of arginine, unravel in several days into 5-20 µ long, beaded strands termed nucleolar necklaces (NN). (silverchair.com)
95-99% pure populations of heterokaryons formed between undifferentiated rat myoblasts and differentiated chick myocytes were obtained. (elsevierpure.com)

Finally, minidystrophintransfection of both types of myoblasts was utilised to examine the effect, especially in dystrophin-deficient myoblasts. (bham.ac.uk)

In conclusion, this study shows that myoblasts derived from both clinically unaffected and affected muscles of patients with FSHD are more susceptible to oxidative stress than control myoblasts. (fshfriends.org)

The earliest detectable event leading to human myoblast differentiation is an upregulation of Kir2.1 channel activity, which causes a negative shift (hyperpolarization) of the resting potential of myoblasts. (unige.ch)

Our results revealed that patients' myoblasts accumulate large vacuoles. (escholarship.org)

Therefore, these abnormalities could be responsible for the muscle weakness observed in patients with FSHD and provide an important marker for FSHD myoblasts. (fshfriends.org)

Myoblast differentiation is essential to skeletal muscle formation and repair. (unige.ch)

Invited review: myoblast transfer: a possible therapy for inherited myopathies? (nih.gov)

Anatomy19

Organisms4

Diseases7

Chemicals and Drugs32

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes24

Disciplines and Occupations1

Information Science3

C2C1212

Myotubes7

Vitro5

Transplantation10

Cells12

Proliferation6

Terminal differentiation1

Skeletal myocytes1

Myogenic3

Mitochondrial3

Drosophila2

Myotube2

Gene1

Fusion2

Regeneration2

Phenotype1

Myofibers1

Molecular1

Cellular1

Intrinsic1

Myosin1

Differentiation Medium1

Expression2

Zinc1

Membrane1

Primary5

Progression1

Chick2

Examine1

Conclusion1

Resting potential1

Results1

Patients1

Formation1

Review1