Myelogenous leukemia k562 cells. Medical search. Frequent questions

Flow cytometric analysis also demonstrated that GLA caused dose-dependent apoptosis of K562 cells. (omega-research.com)
It led to loss of Δψm, release of cytochrome c, activation of caspases-9, -3 and cleavage of PARP, which suggested that bruceine D induced apoptosis of K562 cells through mitochondrial pathway. (edu.hk)

We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)

GLA treatment could also elevate lipid peroxidation in K562 cells, and antioxidant alpha-tocopherol could reverse the cytotoxicity of GLA. (omega-research.com)
Bruceine D exhibited potent cytotoxicity to K562 cells with IC50 of 6.37 ± 0.39 μM. (edu.hk)
In this study, we found that lapatinib induced cytotoxicity in human hepatoma Huh7, HepG2 and HA22T cells. (oncotarget.com)
Utilization of JNK inhibitor SP600125 or knockdown of JNK by siRNA potentiate the cytotoxicity of C-2 through down-regulation of p62 and LC3II proteins and up-regulation of active-Caspase3 proteins, enhance the cell death effect, facilitating the switch from autophagy to apoptosis. (biomedcentral.com)
It reveals a series of molecular mechanisms about SP600125 potentiate the cytotoxicity and tumor inhibition of C-2 in bladder cancer cells through promoting C-2-induced apoptosis, expecting it provides research basis and theoretical support for new drugs development. (biomedcentral.com)
Our previous study reported that a new series of Jaspine B derivatives were designed and synthesized, among them, compound 7f was discovered as an autophagy inducer is associated with the up-regulation of LC3 and Beclin-1, and showed the best overall cytotoxicity on PC-3 cells [ 3 ]. (biomedcentral.com)
And in that article, another compound 7 g (Fig. 1 a, Fig. 2 ) also has significant cytotoxicity and could induce cell autophagy, due to the efficiency of Jaspine B derivatives was investigated in bladder cancer cells rarely, and the specific autophagy effect of compound 7f in PC3 cells had not been investigated deeply. (biomedcentral.com)
In cisplatin-resistant cell lines, BT potentiated cisplatin-induced cytotoxicity at most drug ratios via enhanced ROS generation and modulation of key regulators of apoptosis. (biomedcentral.com)
Met dramatically enhanced ADM cytotoxicity and accelerated apoptosis of MG-63/ADM cells. (bvsalud.org)
Moreover, the cytotoxicity of VCR-encapsulated MRK-Lip to K-562/ADM cells was higher than that of VCR-encapsulated IgG-Lip and Cont-Lip. (elsevierpure.com)
We evaluated the cytotoxicity of dimethylsphingosine (DMS) against four human leukemia cell lines: two acute (HL60 and a multi-drug resistance MDR-positive derivative HL60-dox) and two blast crisis chronic myelogenous leukemias (JFP1, from a treatment refractory patient and K562), and against blasts isolated from 11 leukemia patients. (elsevierpure.com)

Many factors and components play a role in the cell cycle of K562 cells in terms of growth, cell differentiation, and apoptosis. (wikipedia.org)
The growth of these leukemic cells are controlled by either initiating cell differentiation or apoptosis to occur. (wikipedia.org)
Cell differentiation is induced by the deacetylase activity in these "undifferentiated progenitor cells," which alters the phenotype and morphology of the K562 cells. (wikipedia.org)
This gene targets the cyclin-dependent kinase inhibitor, p21, and causes cell differentiation, cell cycle arrest in G1, and ultimately apoptosis. (wikipedia.org)
Other methods being focused on in the regulation of K562 cells include therapeutic methods like polyphyllin D, which caused differentiation from the progenitor state to occur, and for apoptosis to begin. (wikipedia.org)
Abaza L, Talorete TP, Yamada P, Kurita Y, Zarrouk M, Isoda H (2007) Induction of growth inhibition and differentiation of human leukemia HL-60 cells by a Tunisian gerboui olive leaf extract. (springer.com)
In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. (molcells.org)
The up-regulation of miR-199b-5p during erythroid differentiation was dependent on the binding of GATA-1 and NF-E2 to its gene locus, which activated its transcription and maintained its high expression level in mature erythroid cells. (molcells.org)
ALA induced erythroid differentiation of human myelogenous leukemia K562 and murine erythroleukemia MEL cells, leads to hemoglobinization and cellular typical maturation. (longdom.org)
Hemoglobin synthesis and assembly is largely dependent on coordinated gene expression control, it is well established that the histone deacetylase inhibitor butyric acid activates transcription of heme synthesis an globin mRNA expression, leading to changes in cell morphology and induction of erythroid differentiation of erythroleukemic cell lines. (longdom.org)
We demonstrated previously that miR-10a is significantly decreased in aged hBM-MSCs and restoration of the miR-10a level attenuated cell senescence and increased the differentiation capacity of aged hBM-MSCs by repressing Krüpple-like factor 4 (KLF4). (biomedcentral.com)
Our group found that the proliferation and differentiation ability of aged human bone marrow mesenchymal stem cells (hBM-MSCs) was decreased whereas cell senescence was increased [ 7 ]. (biomedcentral.com)
However, other studies have suggested that miR-10a may have additional effects other than regulating cell senescence and differentiation. (biomedcentral.com)

When the levels of these components are thrown off, they can either no longer inhibit apoptosis of the cancer cells, a role fulfilled by BCR/ABL, or they cause apoptosis to be induced, in the same vein as Bax and cytochrome C. These components are key in the mitochondria, and due to this, it has been supported that apoptosis uses the mitochondrial apoptosis pathway. (wikipedia.org)
Asgharzade S, Sheikhshabani SH, Ghasempour E, Heidari R, Rahmati S, Mohammadi M, Jazaeri A, Amini-Farsani Z (2020) The effect of oleuropein on apoptotic pathway regulators in breast cancer cells. (springer.com)
A signaling pathway in which ATM/ATR Kinases block cell-cycle progression through stabilization of P53 (tumor protein). (eirmedltd.com)
C-2 exhibits cytotoxic effect on bladder cancer cells, and JNK activated by C-2 triggers autophagy and up-regulates SQSTM1/p62 proteins, contributing to activation of Nrf2 pathway. (biomedcentral.com)
C-2 significantly reduced the viabilities of human bladder cancer cells and induced apoptosis associated with the mitochondrial pathway. (biomedcentral.com)
Recently we demonstrated that downregulation or over expression of ALA dehydratase (ALAD) and porphobilinogen deaminase (PBGD), the second and the third enzymes of the pathway, by specific shRNAs caused a marked alteration in PpIX synthesis in K562 erythroleukemic cells. (longdom.org)
Timosaponin A-III reverses multi-drug resistance in human chronic myelogenous leukemia K562/ADM cells via downregulation of MDR1 and MRP1 expression by inhibiting PI3K/Akt signaling pathway. (biossantibodies.com)
It had been recommended that bikunin and tissues aspect pathway inhibitor-2 exerted their biology actions through a cell surface area receptor-mediated procedure (3 4 Furthermore tissue aspect pathway inhibitor-2 elicits pro-apoptotic signaling pathway in the individual fibrosarcoma cell series (8). (ecolowood.com)
The signaling pathways further induced apoptosis of U937 cells through the mitochondrion-mediated death pathway. (ecolowood.com)
Collectively our data elucidate a novel ADAM17/Lyn/Akt signaling pathway in keeping the viability of leukemia cells and suggest a strategy in improving leukemia therapy through suppression of ADAM17 protein expression. (ecolowood.com)
Through lentivirus-mediated upregulation of miR-10a and downregulation of KLF4 in aged hBM-MSCs in vitro, we revealed that miR-10a decreased hypoxia-induced cell apoptosis and increased cell survival of aged hBM-MSCs by repressing the KLF4-BAX/BCL2 pathway. (biomedcentral.com)
They also found that downregulation of miR10a/10b in clonal cells interfered with cell proliferation and enhanced cell apoptosis by activating the NF-κB-dependent p53 pathway [ 13 ]. (biomedcentral.com)

Chronic myeloid leukemia (CML), an acquired malignant myeloproliferative disorder of hematopoietic stem cells, is one of the three most common forms of leukemia. (edu.hk)

Furthermore, activation of autophagy allows to extend cell survival when exposed to different types of stressors such as starvation or cytotoxic drugs. (hindawi.com)
Rab27A is expressed in many human tissues and it regulates the secretion of several types of cellular vesicles, such as melanosomes, insulin granules of pancreatic beta cells, and lytic granules of cytotoxic T lymphocytes. (bdbiosciences.com)

Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. (molcells.org)
Heme synthesis enhances globin formation, not only because it is the precursor of Hb but also by modulating the machinery of gene expression and globin synthesis and coordinating erythroid cell maturation. (longdom.org)

K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. (wikipedia.org)

In fact, many studies have demonstrated the oleuropein and its derivative have proved pharmacological activity against proliferation of cancer cells and several tumor cell lines by different mechanisms such arrest cell cycle and cause apoptosis in cancerous cells, by modulation of miRNA expression and upregulation and downregulation of several genes. (springer.com)
This product inhibits the proliferation of human chronic myelogenous leukemia k562 cells. (eirmedltd.com)
These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. (biossantibodies.com)
Bosutinib is a potent inhibitor of CML cell proliferation in vitro and has demonstrated promising activity in CML patients resistant or intolerant to IM as well as in newly diagnosed patients with chronic phase CML (CML-CP). (mdm2-receptor.com)

MTT assay was used to evaluate cell growth inhibition. (edu.hk)
To validate the ER tension model and assess cell survival pursuing thapsigargin challenge, proteins markers for ER tension and apoptosis aswell as cell success had been evaluated using American blot evaluation and MTT assay. (health-e-nc.org)
b The effect of C-2 in reducing cell viabilities of bladder cancer cells (BIU87, EJ and 5637) measured by MTT assay. (biomedcentral.com)
Potentiation of vincristine-induced growth inhibition by these MFs was also tested in K562/ADM cells by means of WST-1 [2-(4-iodophenyl)-3-(4- nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] assay. (elsevierpure.com)
4 xCELLigence real-time cell analysis 6 Diverse cancer immunotherapy applications 6 How does the xCELLigence real-time cell analysis assay work? (technologynetworks.com)
While many methods have been developed to screen and evaluate the efficacy of immune cell‑mediated killing, there is a need for a more robust in vitro assay to accurately predict the in vivo behavior of therapies. (technologynetworks.com)
The most commonly used method for measuring immune cell‑mediated killing is the release assay, where effector cell‑mediated disruption of the target cell membrane results in leakage of its cytoplasmic contents into the culture medium. (technologynetworks.com)

These results demonstrate that GLA could induce apoptosis in K562 cells. (omega-research.com)
It can inhibit stages in the cancer cell, prevent cell cycle progression, inhibit cell growth and induce apoptosis. (eirmedltd.com)

Multidrug resistance parameters, tissue infiltration parameters, receptors for colony-stimulating factors (CSFr) and cell cycle parameters were analyzed using flow cytometry in 145, 109 initial and 36 relapsed or refractory, acute nonlymphoblastic leukemia (ANLL) patients to find out clinically more reliable functional parameters. (karger.com)

Flow cyt: U87,and HeLa cells. (abcam.com)
ICC: HeLa, and K562 cells. (abcam.com)
It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)

K562 (chronic myelogenous leukemia, ATCC) and A549 (lung carcinoma, ATCC) cell lines had been used as detrimental handles. (bso14.org)

Gamma-linolenic acid induces apoptosis and lipid peroxidation in human chronic myelogenous leukemia K562 cells. (omega-research.com)

Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)

Other established methods, such as flow cytometry, can then provide extra data regarding immune cell phenotype, activation, and function. (technologynetworks.com)

In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)

The change in phenotype induces a decrease in the growth rate and leads the K562 cells to the terminal path of becoming mature erythroids, monocytes, and mature macrophages. (wikipedia.org)

Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. (bdbiosciences.com)
The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27Kip1. (bdbiosciences.com)
Moreover, lapatinib-induced autophagy in hepatoma cells was confirmed by the detection of autophagic LC3-II conversion, the up-regulation of autophagy-related proteins, and the down-regulation of p62 by immunoblotting. (oncotarget.com)
In addition, the shRNA of knocked-down autophagy-related proteins rescued the hepatoma cells from lapatinib-induced growth inhibition. (oncotarget.com)
d Active-casepase 3, PARP and CF-PARP proteins expression were detected by western blotting at a dose- and time- dependent manner in BIU87 cells. (biomedcentral.com)
Because soybean Kunitz-type trypsin inhibitor has been found to induce apoptotic death of human being leukemia Jurkat cells (17) anti-leukemia activity of B. multicinctus protease inhibitor-like proteins is definitely therefore examined. (ecolowood.com)

Chemotherapy is one of the most important treatment options in the treatment of all types of leukemia, but multi-drug resistance often precludes the success of this treatment. (ijpsonline.com)
However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
The results of qRT-PCR and Western blotting demonstrated that the expression level of Yin Yang 1 (YY1) and multi-drug resistance-1 (MDR1) in MG-63/ADM cells were remarkably increased compared with those in MG-63 cells. (bvsalud.org)

K562 cells were the first human immortalised myelogenous leukemia cell line to be established. (wikipedia.org)
K562 cells are part of the NCI-60 cancer cell line panel used by the National Cancer Institute. (wikipedia.org)
Another important group of regulators of the K562 line are Sirtuins, referred to as SIRTS. (wikipedia.org)
The Leukemia-Lymphoma Cell Line Factsbook. (wikipedia.org)
Lysate from the K-562 cell line (Cat. (bdbiosciences.com)
Three studies have shown that monoclonal antibodies to the DENV fusion loop epitope reliably enhanced Zika virus infection (i.e., antibody-dependent enhancement [ADE]) in Fc receptor-bearing K-562 human myelogenous leukemia cells or the U-937 human monocytic cell line ( 1 - 3 ). (cdc.gov)
In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
We examined the effect of MRK-16, a monoclonal antibody against P-gp, modified liposomes (MRK-Lip) on the human myelogenous leukemia K-562 cells and its adriamycin resistance cell line K-562/ADM cells to avoid the side effects and to reverse the multidrug resistance. (elsevierpure.com)
Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases. (ubigene.us)
This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)

In the current study, we tested the apoptotic effect of GLA on human chronic myelogenous leukemia K562 cells. (omega-research.com)
Typical apoptotic nuclei were shown by staining of K562 cells with DNA-binding fluorochrome Hoechst 33342, characterized by chromatin condensation and nuclear fragmentation. (omega-research.com)
For the mode of cell death, we found lapatinib induced a higher percent of dead cells and a lower percent of hypodiploid cells, suggesting non-apoptotic cell death in lapatinib-treated hepatoma cells. (oncotarget.com)
Enhanced apoptosis was correlated with loss of pro-survival factors (XIAP, bcl-2, bcl-xL), expression of pro-apoptotic markers (caspases 3/7, PARP cleavage) and enhanced cell cycle regulators p21 and p27. (biomedcentral.com)

One of the most effective bispecific antibodies in scientific research are bispecific T-cell engagers (BITEs), which contain 2 single string adjustable fragments (scFVs) linked by a brief linker15. (bso14.org)

Cell Culture Individual severe myelogenous leukemia U937 cells and individual chronic myelogenous leukemia K562 cells extracted from ATCC (Manassas VA) had been grown up in RPMI 1640 moderate supplemented with 10% fetal leg serum (Invitrogen) 2 mm l-glutamine penicillin (100 systems/ml)/streptomycin (100 μg/ml) and 1% sodium pyruvate incubated at. (ecolowood.com)
Materials and Strategies Cell lines and lifestyle circumstances The Ph-positive severe B lymphoblastic leukemia (ALL) cell series BV173 (German Assortment of Microoganisms and Cell Civilizations, Braunschweig, Germany) and Burkitts lymphoma cell lines Daudi and Raji (ATCC, Manassas, VA) Zoledronic Acid had been used as Compact disc19-positive goals. (bso14.org)
293T cells (ATCC) had AKAP10 been employed for product packaging retroviral vectors and harvested in DMEM. (bso14.org)

Compare clinical response, in terms of 1-year progression-free survival and rate of molecular complete remission, in patients with Philadelphia chromosome-positive chronic myelogenous leukemia (Ph+ CML) in chronic phase who have achieved a complete cytogenetic remission to single-agent tyrosine kinase inhibitor treated with interferon alfa and sargramostim (GM-CSF) vs tyrosine kinase inhibitor and GM-K562 cell vaccine. (knowcancer.com)
Autophagic cell death was demonstrated by images of punctuated LC3 patterns, a higher percent of acridine orange positive cells, as well as a partial rescue of cell death by autophagy inhibitor 3-methyladenine or chloroquine. (oncotarget.com)
With this study human being leukemia U937 cells were treated with B. multicinctus protease inhibitor like protein-1 (PILP-1). (ecolowood.com)

Among all the studied palladium(II) complexes, the [Pd(TSC3)2] (8) complex exhibited high antitumor activity on the DU145 prostate carcinoma and K562 chronic myelogenous leukemia cells, with low values of the inhibitory concentration (0.01 and 0.02 𝜇M, resp. (edu.pe)
In this scholarly study, we characterize ENG T cells particular for Compact disc19-positive malignancies (Compact disc19-ENG T cells) and present they are turned on and eliminate tumor cells within an antigen reliant manner, have the ability to recruit bystander T cells to tumor cells, and also have antitumor activity in preclinical versions. (bso14.org)

Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells. (biossantibodies.com)

It helps to target multiple signaling pathways in cancer cells without presenting any toxicity to normal cells. (eirmedltd.com)
In vivo study, C-2 suppresses tumor growth in a xenograft mouse model of EJ cells without observed toxicity. (biomedcentral.com)
Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. (thermofisher.com)
Spectacular increase in transfection efficiency and dramatic decrease in cell toxicity. (thermofisher.com)

The leaf extracts and its derivative compounds have shown potent anticancer effects against different cell lines in different organs. (springer.com)

Dano, K. Active outward transport of daunomycin in resistant Ehrlich ascites tumor cells. (nature.com)
eliminating, and redirected bystander T cells to tumor cells. (bso14.org)

Among others Oleuropein and its derivative compounds (hydroxytyrosol, tyrosol and others) were studied in a several types of cancer cells. (springer.com)
Compounds (C1-8 and doxorubicin) were placed to 96-well culture plate in triplicate order at doses of 1, 10, 100, 1000 μM and then K562 cells were seeded into the wells at the dose of 105/ml. (ijpsonline.com)

These results suggest that the interaction between liposomes and multidrug resistance cells was increased by the modification of liposomes with MRK-16. (elsevierpure.com)

3.3 Aftereffect of ER strain and ALDH2 on myocardial histology, ER strain and cell survival To measure the impact 287714-41-4 manufacture of ALDH2 transgene on myocardial histology subsequent ER strain induction, cardiomyocyte cross-sectional area and interstitial fibrosis had been examined. (health-e-nc.org)

Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
Aging is one of the key factors that regulate the function of human bone marrow mesenchymal stem cells (hBM-MSCs) and related changes in microRNA (miRNA) expression. (biomedcentral.com)
Previous studies proved that bone marrow (BM) stem cells play an important role in improving heart function and delaying cardiac remodeling [ 1 , 2 ] after ischemic damage through reducing fibrosis and increasing angiogenesis. (biomedcentral.com)

Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
Arm II: Patients receive GM-K562 cell vaccine intradermally once every 3 weeks for a minimum of 6 months. (knowcancer.com)
13 ] found that miR-10a and miR-10b were significantly increased in CD34 + marrow cells from 28 patients with myelodysplastic syndrome compared with healthy donors. (biomedcentral.com)

The uptake of vincristine (VCR) by K-562/ADM cells was lower than that by K-562 cells. (elsevierpure.com)
MFs increased the uptake of [ 3 H]vincristine into MDR cells and exhibited MDR-reversing effects. (elsevierpure.com)

Myers lab K562 growth 5 was used in control libraries SL30827 (fragmentation date 04-08-13) and SL12793 (fragmentation date 03/23/12). (encodeproject.org)
The suppression of the NF-kB transcription factor activation is involved in inhibiting tumor cell growth by inducing apoptosis. (eirmedltd.com)
In conclusion, lapatinib induced autophagic cell death and the growth of human hepatoma cells. (oncotarget.com)
Moreover, the capacity to continue cell growth at the moment of virus infection may be important as the applied MOI was 0.01 which means 99% of the cells will not be infected during the first virus replication cycle and can potentially grow further. (tyrosinekinases.com)

Biedler, J. L. & Riehm, H. Cellular resistance to actinomycin D in Chinese hamster cells in vitro: cross-resistance, radioautographic, and cytogenetic studies. (nature.com)

Using cocultures of hCMEC/D3 wildtype cells and cells transduced with MDR1-EGFP or monocultures of hCMEC/D3-MDR1-EGFP cells, we found release of both Pgp-enriched exosomes and microvesicles but analysis of the exosomal marker protein Rab7 indicated that doxorubicin increased particularly the release of exosomes. (bvsalud.org)

Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-g, TNF-a and IL-10, while in vivo ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model. (rndsystems.com)
Here, the role of a new autophagy inducer Jaspine B derivative C-2 in suppressing bladder cancer cells was researched in vitro and in vivo. (biomedcentral.com)
The hypoxia-induced apoptosis, cell survival, and cell paracrine function of aged hBM-MSCs were investigated in vitro. (biomedcentral.com)

The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes and erythrocytes. (wikipedia.org)
There are many different cellular components involved in the cycle of apoptosis such as BCR/ABL, Bcl-2, Bax protein, and cytochrome C. The tumor suppressor gene p53 is also important in the cell cycle regulation of K562 cells. (wikipedia.org)
Cells grow and divide as a part of the body's natural process, when the Tumor Suppressing Gene (TSG) gets damaged, (TSG is a gene that regulates a cell during cell division and replication. (eirmedltd.com)
In addition, the BCR-ABL fusion gene product, a constitutively activated tyrosine kinase which is crucial for the development of chronic myeloid leukemia (CML), is highly sensitive to bosutinib. (mdm2-receptor.com)
At the same time, we already provide customers with high quality gene-editing tools for cell or animal research worldwide. (ubigene.us)

HL60-dox and JFP1 were the most sensitive, indicating DMS efficacy against human leukemia MDR. (elsevierpure.com)

18,21 Early in 2010, a team of Taiwanese researchers discovered that GLA regulates the inflammatory "master molecule" nuclear factor-kappaB or NF-kB, preventing it from switching on genes for inflammatory cytokines in cell nuclei. (lifeextension.com)
Cancer immunotherapies can be divided into four major categories: - Cytokines/immunomodulation agents - Monoclonal antibodies - Cell‑based therapies - Oncolytic viruses Though monoclonal antibodies currently represent the largest class of commercialized cancer immunotherapies, cell‑based therapies are rapidly making headway. (technologynetworks.com)

Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)

Low doses of BT and cisplatin enhanced efficiency of cisplatin treatment in all the ovarian cancer cell lines tested. (biomedcentral.com)

To establish adriamycin (ADM)-resistant MG-63 (MG-63/ADM) cells, the dosage of ADM was progressively increased. (bvsalud.org)

These play a role in cellular stress, metabolism, and autophagy, by interacting with deacetylases activity in the cell. (wikipedia.org)
Autophagy is an intracellular degradation system that ensures a dynamic recycling of a variety of building blocks required for self-renewal, homeostasis, and cell survival under stress. (hindawi.com)
Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)
While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)
Therefore, compound 7 g was selected and given chemical name of C-2 to further research autophagy mechanism and its effect on bladder cancer cells and to evaluate its antitumor activities in this study. (biomedcentral.com)
C-2 induced autophagy of bladder cancer cells. (biomedcentral.com)
a The expression levels of C-2-induced autophagy related protein Beclin-1, LC3, p62, ATG3, ATG5 and ATG7 were detected by western blotting in BIU87 and EJ cells at the indicated concentrations or treated with 4 μM of C-2 at the indicated time points. (biomedcentral.com)
b The expression levels of C-2-induced autophagy related protein Beclin-1, p62 and LC3 were detected by western blotting in EJ cells at the indicated concentrations. (biomedcentral.com)

The problem with K562 cells, and many other cancer cell types, is an overabundance of Aurora kinases. (wikipedia.org)
Inhibiting these is an important regulation mechanism of cancer, because it prevents cells from progressing into mitosis. (wikipedia.org)
The ability to induce these changes in K562 cell cycle and cell cycle regulation provides targets for cancer drugs. (wikipedia.org)
Cancer cells frequently display intrinsic or acquired resistance to chemically diverse anticancer drugs, limiting therapeutic success. (nature.com)
In cancer biology, controlling leukemia remains a big challenge. (ijpsonline.com)
It also inhibits migration and invasion of human cervical cancer cells. (eirmedltd.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The underlying mechanisms and anticancer effect of C-2 in bladder cancer cells were investigated by MTT, western blotting, immunoprecipitation and immunofluorescence assays. (biomedcentral.com)
Antitumor effects of the silencing of programmed cell death ligand 1 in colorectal cancer via immunoregulation. (biossantibodies.com)
This class of personalized therapies involves collecting immune cells from an individual, engineering them to recognize and kill cancer cells, before culturing, and reintroducing them into the same individual. (technologynetworks.com)

In the brain, the efflux transporter P-glycoprotein (Pgp) is predominantly located on the luminal membrane of microvascular endothelial cells (BMECs) that form the blood-brain barrier. (bvsalud.org)

Anatomy13

Organisms4

Diseases31

Chemicals and Drugs31

Analytical, Diagnostic and Therapeutic Techniques and Equipment13

Phenomena and Processes17

Disciplines and Occupations1

Information Science2

Health Care2

Apoptosis of K562 cells2

Acute myeloid1

Cytotoxicity11

Differentiation13

Pathway12

Hematopoietic Stem1

Cytotoxic2

Erythroid cell2

Erythroleukemia1

Proliferation4

Assay7

Induce apoptosis2

Refractory1

HeLa5

A5491

Induces apoptosis1

Mitochondrial2

Flow Cytometry1

Morphology1

Phenotype1

Proteins6

Multi-Drug Resis3

Line11

Apoptotic4

Bispecific T-cell engagers1

ATCC3

Inhibitor3

Antitumor activity2

Malignant1

Toxicity4

Potent1

Tumor cells2

Compounds2

Multidrug resistance1

Induction1

Bone3

Patients3

Vincristine2

Growth4

Cytogenetic1

MDR11

Vitro3

Gene5

Human leukemia1

Cytokines2

Monoclonal antibodies1

Ovarian cancer cell1

Adriamycin1

Autophagy8

Cancer10

Membrane1