Protein EngineeringTissue EngineeringPoint MutationGenetic EngineeringMutationMutation, MissenseModels, MolecularDNA ShufflingMetabolic EngineeringMutagenesis, Site-DirectedMolecular Sequence DataAmino Acid SequenceDirected Molecular EvolutionProtein ConformationProtein FoldingSubtilisinBiomedical EngineeringEscherichia coliAmino Acid SubstitutionFrameshift MutationMutagenesisInteinsProtein Structure, TertiaryBase SequenceProtein StabilityBinding SitesProtein Structure, SecondaryKineticsGerm-Line MutationProteinsEnzyme StabilityRecombinant ProteinsMutant ProteinsBiotechnologyStructure-Activity RelationshipProtein SplicingSynthetic BiologyCrystallography, X-RayTissue ScaffoldsPedigreePeptide LibraryProtein BindingSubstrate SpecificityBacterial ProteinsPolymerase Chain ReactionRecombinant Fusion ProteinsBiophysical ProcessesSequence AlignmentDNA Mutational AnalysisThermodynamicsSequence Homology, Amino AcidMutation RateDNA PrimersCatalysisBacteriophage M13Phosphoribosylglycinamide FormyltransferaseCloning, MolecularBiocompatible MaterialsPlasmidsCell Surface Display TechniquesProtein DenaturationSequence Analysis, DNAExonsTyrosine-tRNA LigaseHeterozygoteCircular DichroismBioengineeringSequence DeletionGenes, Syntheticalpha-AmylasesEnzymesTemperatureHydroxymethyl and Formyl TransferasesAllelesCell EngineeringSaccharomyces cerevisiaeCysteineDNABacillusPeptidesCell LineCatalytic DomainPolymorphism, Single-Stranded ConformationalChemical EngineeringBiosensing TechniquesTriose-Phosphate IsomeraseGene LibraryDrug DesignComputer SimulationTitrimetryModels, ChemicalGenotypeCodon, NonsenseHomozygoteCrystallizationGeobacillus stearothermophilusDisulfidesMembrane ProteinsNuclear Magnetic Resonance, BiomolecularComputational Biology