Mutation conserved sequence. Medical search. Frequent questions

While accumulation rates of nonsynonymous mutations differed between genes and hexapod groups, we found no effect of reproductive mode on the effectiveness of purifying selection acting at nonsynonymous and synonymous sites. (nih.gov)
In the absence of antigenically matched hemagglutinin-based vaccines, DNA vaccination with conserved influenza genes may provide a useful first line of defense against a rapidly spreading pandemic virus. (cdc.gov)
RNA sequencing revealed the upregulation of inflammasome pathway-related genes, keratinocyte stress marker genes, and keratinocyte differentiation marker genes in the Nlrp1b KI mice after UVB irradiation. (frontiersin.org)
Thus mutations in the Pax genes are important causes of human developmental defects. (nih.gov)
Conserved sequences outside of known genes are likely to be gene regulatory elements. (sciencedaily.com)
Because regulatory elements are typically located near the genes they govern, the researchers assigned each conserved element to the closest gene. (sciencedaily.com)
In the first period of evolutionary innovation, in addition to changes affecting developmental genes, the study found a dramatic enrichment in conserved elements near genes for proteins known as "transcription factors," which bind to DNA and regulate whole groups of other genes. (sciencedaily.com)
Only about 10,000 of these genes have been annotated by the sea urchin sequencing team. (blogspot.com)
Pachyonychia congenita is a rare genodermatosis due to mutations in one of four keratin genes. (medscape.com)
Pachyonychia congenita type 1, or the Jadassohn-Lewandowsky type (Mendelian Inheritance in Man (MIM entry 167200), was attributed to mutations in genes encoding keratin 6A (KRT6A) or keratin 16 (KRT16) and constituted the most common form of the disorder. (medscape.com)
The mutations in pachyonychia congenita are found in the genes encoding keratin 6A (KRT6A), keratin 16 (KRT16), keratin 6B (KRT6B), and keratin 17 (KRT17). (medscape.com)
Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)
Whole genome sequencing can also provide insights into the specific genes or mutations that may be linked to antibiotic resistance. (folkhalsomyndigheten.se)
Identification of novel genes by targeted exome sequencing in Retinoblastoma. (cdc.gov)
In addition, long-read sequencing of one representative XDR ST235 isolate identified an integron carrying multiple resistance genes (including bla VIM-2), with differences in gene composition and synteny from the P. aeruginosa class 1 integrons described previously. (who.int)

In evolutionary biology, conserved sequences are identical or similar sequences in nucleic acids (DNA and RNA) or proteins across species (orthologous sequences), or within a genome (paralogous sequences), or between donor and receptor taxa (xenologous sequences). (wikipedia.org)
Conserved proteins undergo fewer amino acid replacements, or are more likely to substitute amino acids with similar biochemical properties. (wikipedia.org)
As with proteins, nucleic acids that are important for the structure and function of non-coding RNA (ncRNA) can also be conserved. (wikipedia.org)
These mutations may also change the distribution of septin-9 isoforms and their interactions with other septin proteins in some of the body's tissues. (medlineplus.gov)
Using a newly developed enzyme-coupled continuous activity assay, we compare differing NAS proteins identified through multiple sequence alignments and phylogenetic analyses. (bvsalud.org)
Aims and objectives: This study aimed to predict and analyse candidate immunogenic epitopes for vaccine development by evaluating the physio-chemical properties, multiple sequence alignment, secondary and tertiary structures, phosphorylation sites, transmembrane domains, and signal peptides, of T. gondii rhoptry proteins ROP7, ROP21, and ROP22 using bioinformatics tools. (bvsalud.org)
Methods: To find immunogenic epitopes of rhoptry proteins, numerous bioinformatics web servers were used containing multiple sequence alignment, physiochemical properties, antigenicity and allergenicity, post-translational modification sites (PTMs), signal peptides, transmembrane domains, secondary and tertiary structures, and screening of predicted epitopes. (bvsalud.org)
This macromolecular assembly comprises more than 20 conserved proteins that form a series of ring-like structures and a needle-shaped protrusion, which allows the transport of proteins into a host cell. (lu.se)

Therefore, to capture the heterogeneity of the HEV quasispecies, including recombination events and SNP that occur with very low frequencies, we developed new sequencing approaches for HEV genotype 3 based on the amplification of the near full-length genome of HEV by long-range PCR (lrPCR) followed by subsequent next-generation sequencing (NGS) and third-generation sequencing. (nature.com)
The Public Health Agency's Next Generation Sequencing (NGS) platform performs massive parallel sequencing of DNA and RNA. (folkhalsomyndigheten.se)

Advances in high-throughput DNA sequencing and protein mass spectrometry has substantially increased the availability of protein sequences and whole genomes for comparison since the early 2000s. (wikipedia.org)
Now researchers combing through the DNA sequences of vertebrate genomes have identified three distinct periods of evolutionary innovation that accompanied this remarkable diversification. (sciencedaily.com)
By comparing the genomes of species whose evolutionary lineages diverged at different times in the past, researchers can see when in evolutionary history a particular conserved sequence first appeared. (sciencedaily.com)
I would be very interested to know if the conclusions of this work would be changed by the ability to determine the sequences of all the viral genomes recovered from a single infected cell . (virology.ws)
To gain in-depth insights into the viral population and to perform detailed characterization of viral genomes, we used a new approach combining long-range PCR with next-generation and third-generation sequencing which allowed near full-length sequencing of HEV-3 genomes. (nature.com)
Furthermore, we developed a targeted ultra-deep sequencing approach to assess the dynamics of clinically relevant mutations in the RdRp-region and to detect insertions in the HVR-domain in the HEV genomes. (nature.com)
The highly repetitive sequence content of these regions poses a major barrier to their molecular analysis, and the PAR and centromeres remain unassembled or incompletely assembled on many of highest quality reference genomes, including mouse. (jax.org)

The extent to which a sequence is conserved can be affected by varying selection pressures, its robustness to mutation, population size and genetic drift. (wikipedia.org)
In coding sequences, the nucleic acid and amino acid sequence may be conserved to different extents, as the degeneracy of the genetic code means that synonymous mutations in a coding sequence do not affect the amino acid sequence of its protein product. (wikipedia.org)
They are suggesting that there's a "rich array" of mysterious sequences that affect genetic diseases. (blogspot.com)
The mutations that produce genetic defects in humans will almost certainly turn out to be in well-understood parts of the gene or its closely associated regulatory sequences. (blogspot.com)
We also demonstrate that self-propagating active genetic elements (CopyCat elements) can efficiently delete and replace the L2-CRM with orthologous sequences from other divergent fly species. (elifesciences.org)
Mutations that disrupt centromere integrity or reduce homology between X- and Y-linked PARs can lead to chromosome segregation errors and constitute important genetic mechanisms for cancer, cellular senescence, and infertility. (jax.org)
Biological information analysis revealed the mutation was pathogenic and preimplantation genetic diagnosis was adopted. (researchsquare.com)
Conclusion Genetic testing prior to pregnancy is recommended in Alport syndrome patients or family members prior to pregnancy to determine the specific location of the gene mutation. (researchsquare.com)
Genetic analysis of patients with leukocyte adhesion deficiency: genomic sequencing reveals otherwise undetectable mutations. (lu.se)
Metagenomic sequencing engables a thorough mapping of the genetic material in the clinical sample, providing a broader understanding of the microbiological profile. (folkhalsomyndigheten.se)
In contrast to metagenomic detection of unknown pathogens which sequences all available genetic material, 16S sequencing specifically targets the small ribosomal subunit. (folkhalsomyndigheten.se)
By sequencing the entire genome, a comprehensive understanding of the genetic composition is obtained. (folkhalsomyndigheten.se)
In epidemiological contact tracing, this technology enables the mapping of infection spread and identification of sources of infection through the comparison of genetic sequences. (folkhalsomyndigheten.se)

Enzolytics has a robust Artificial Intelligence Platform for genomic surveillance and monitoring of viral epidemiology that is used to identify conserved targets for produced Monoclonal Antibodies. (bignewsnetwork.com)
Because the genomic structure of NPC1 was unknown, initial mutation screening was performed on RT-PCR products or partial genomic amplicons. (bmj.com)
8 Mutation screening using RT-PCR products has several drawbacks compared with screening using genomic amplicons. (bmj.com)
Furthermore, using sequence analysis of human genomic DNA, researchers concluded that the amino acids important for catalysis by COX-1 are conserved and are equally important for catalysis by COX-2. (medscape.com)

Whole exome sequencing identifies a novel compound heterozygous GFM1 variant underlying developmental delay, dystonia, polymicrogyria, and severe intellectual disability in a Pakhtun family. (nih.gov)
Background De novo mutations are emerging as an important cause of neurocognitive impairment, and whole exome sequencing of case-parent trios is a powerful way of detecting them. (bmj.com)
Methods The Deciphering Developmental Disorders study is using whole exome sequencing in family trios to investigate children with severe, sporadic, undiagnosed developmental delay. (bmj.com)
Whole-exome data of the COL4A5 gene of the patient's family were analyzed using Sanger sequencing and online software was used to predict the conservation and pathogenicity of mutant amino acids within species evolution. (researchsquare.com)

It is a platform and database for the automated analysis of whole genome sequencing data. (folkhalsomyndigheten.se)

Multiple missense mutations are required to block CIF2 binding in vitro and GSO1/SGN3 function in vivo. (unige.ch)
1981 ). Although more than 400 mutations have been reported in the COL4A5 gene, of which missense mutations account for 30%, there are no hotspot mutations. (researchsquare.com)

Results Protein-altering de novo mutations in PURA were identified in four subjects. (bmj.com)
Two Novel Frame Shift, Recurrent and De Novo Mutations in the ITGB2 (CD18) Gene Causing Leukocyte Adhesion Deficiency in a Highly Inbred North African Population. (lu.se)

Over many generations, nucleic acid sequences in the genome of an evolutionary lineage can gradually change over time due to random mutations and deletions. (wikipedia.org)
Speaking on the role of Monoclonal Antibodies in targeting conserved sequences for Monkeypox, Dr. Suraj Saggar, an Advisory Board Member, said, 'Monkeypox is a DNA virus and is less prone to random mutations during replication versus an RNA virus-like SARS-Cov2. (bignewsnetwork.com)

DDX58/RIG-I, a sensor of cytoplasmic viral RNA and toll-like receptors (TLR) 3, 7, and 8, which bind RNA in endosomes, are conserved in pangolins. (frontiersin.org)
Wuhan strain will continue to arise, yet not all viral variants generated through mutation of the virus are important or have immediate public health relevance. (who.int)
To date, WHO has designated 4 SARS CoV-2 genotypes that meet the working definition of a VOC: i) contain mutations divergent from the Wuhan strain and ii) that the mutations impact one or more of the aspects of viral infection that have public health significance. (who.int)
Patient A had no identified risk factor for HIV infection and was infected with a strain of HIV closely related to that of her dentist as determined by viral DNA sequencing. (cdc.gov)

A few SEPTIN9 gene mutations have been identified in individuals with hereditary neuralgic amyotrophy, a disorder characterized by episodes of severe pain and muscle wasting (amyotrophy) in the shoulders and arms. (medlineplus.gov)
However, the relation between SEPTIN9 gene mutations and immune function is unclear. (medlineplus.gov)
Analysis of GFM1 gene mutations in a family with combined oxidative phosphorylation deficiency 1]. (nih.gov)

We analyse the accumulation of slightly deleterious nonsynonymous and synonymous point mutations in 99 single copy orthologue protein-coding loci shared among the investigated taxa. (nih.gov)
Point mutations cluster into the positively charged end of the molecule around the predicted binding site for phosphatidylinositol lipids. (embl.de)
Overall, these assays and approaches detect specific mutations or features such as insertions, deletions and point mutations in the SARS CoV-2 genome that are characteristic of a particular VOC/VOI using PCR. (who.int)

[ 5 , 6 ] have provided a clearer picture of the disease and reveal a spectrum of overlapping clinical features that can be correlated genotypically to the specific mutations in patients with pachyonychia congenita. (medscape.com)
However, variants with specific mutations that affect the way the virus behaves are now considered either Variants of Interest (VOI) or Variants of Concern (VOC) with respect to public health implications (1). (who.int)

Mutation screening of all coding regions and flanking intron sequences of the RP1L1 gene were performed with DNA sequencing analysis in this case with OMD. (molvis.org)
Our analysis using 386K10 confirmed the exon/intron boundary sequences reported by Morris et al 14 and complements their data by showing the lengths of introns 1 (20 kb) and 6 (3 kb). (bmj.com)
Sets of primers to amplify each of the 25 exons of NPC1 were designed according to the corresponding intron sequences (table 1 ). (bmj.com)

For contact tracing or inquiries related to antibiotic resistance, whole genome sequencing (WGS) of bacterial isolates is offered. (folkhalsomyndigheten.se)

Nucleic acid sequences that cause secondary structure in the mRNA of a coding gene may be selected against, as some structures may negatively affect translation, or conserved where the mRNA also acts as a functional non-coding RNA. (wikipedia.org)
For example, mutations that reduce the mRNA stability may escape the screening. (bmj.com)
For this aim, primers were designed to amplify the enolase gene from the F. hepatica enolase sequence, mRNA was isolated from F. hepatica adult fluke obtained from an infected sheep followed by cDNA was obtained. (bvsalud.org)

Homology search tools may take an individual nucleic acid or protein sequence as input, or use statistical models generated from multiple sequence alignments of known related sequences. (wikipedia.org)

COLLEGE STATION, TX / ACCESSWIRE / June 21, 2022 / Enzolytics, Inc. (OTC PINK:ENZC) (ENZC' or the 'Company') today announced it has identified conserved, immutable sites (epitopes) on the Monkeypox virus. (bignewsnetwork.com)
Tecovirimat is a virostatic agent that targets a major envelope protein conserved across orthopoxviruses (VP37 in monkeypox virus). (cdc.gov)

Figure 1 Homology in domain structures and amino acid sequences between human and mouse NLRP1 and the present knock-in mutation. (frontiersin.org)

The new study identified millions of these regulatory innovations by using computational methods to look for DNA sequences that are still the same in species that have evolved separately over long periods of time. (sciencedaily.com)
A commonly discussed mechanism was variable levels of gene expression, but both Blencowe and Chris Burge,,, found that gene expression is relatively conserved among species. (uncommondescent.com)
The serine at position 1199 is well conserved among the RP1L1 family in other species. (molvis.org)
used the CopyCat tool to delete the regulatory sequence and replace it with sequences from three other species of flies. (elifesciences.org)
Sequence comparison between VDE from different species were made and particular interest was focused on four highly conserved histidines (H121,124,167,173) and their possible involvement in enzymatic activity. (lu.se)
16S sequencing is performed to determine which bacterial species are present in a clinical sample or isolate. (folkhalsomyndigheten.se)

By making deletions in the β loop we defined a 20 amino-acid sequence that is sufficient for clustering. (jneurosci.org)

The mechanisms of heterosubtypic immunity are not completely understood but likely include both T-cell immunity, in particular CD8+ cytotoxic T-lymphocytes (CTL) ( 16 , 17 ) and CD4+ T cells (13) , as well as antibodies to conserved epitopes (18) . (cdc.gov)
The analysis of these conserved sequences revealed that all epitopes identified by the Company are linear, accessible by antibodies, and unaffected by mutations. (bignewsnetwork.com)

Conservation can occur in coding and non-coding nucleic acid sequences. (wikipedia.org)
The nucleic acid sequence of a protein coding gene may also be conserved by other selective pressures. (wikipedia.org)
All nucleic acid in the clinical sample is sequenced and the resulting data is analysed by experienced bioinformaticians. (folkhalsomyndigheten.se)

For example, the promoter of a conserved gene or operon may also be conserved. (wikipedia.org)
In 1987 the sdhCAB operon of B. subtilis was cloned and sequenced. (lu.se)

The associated human phenotype of de novo heterozygous mutations in this gene is variable, but moderate to severe neurodevelopmental delay and learning disability are common to all. (bmj.com)
This was verified as a new mutation not present in the parents, whereas the daughter had a heterozygous mutation. (researchsquare.com)

Non-coding sequences important for gene regulation, such as the binding or recognition sites of ribosomes and transcription factors, may be conserved within a genome. (wikipedia.org)
This was not helpful since it included a huge number of sites and sequences that result from spurious (nonfunctional) binding of transcription factors or accidental transcription of random DNA sequences to make junk RNA [see What did the ENCODE Consortium say in 2012? ]. (blogspot.com)

Many functional sequences are also modular, containing regions which may be subject to independent selection pressures, such as protein domains. (wikipedia.org)
2014) points out that the purpose of the ENCODE project was to discover functional sequences in the human genome. (blogspot.com)

The codon usage bias in some organisms may restrict the types of synonymous mutations in a sequence. (wikipedia.org)

Phenotypic analysis of these ' in locus ' mutations based on both expression of Kni protein and adult wing phenotypes, reveals novel unexpected features of L2-CRM function including evidence for a chromosome pairing-dependent process that promotes transcription. (elifesciences.org)

Indeed, we have shown previously that mutation of a tyrosine phosphorylation site in the long cytoplasmic loop of the β subunit impairs agrin-induced cytoskeletal anchoring and aggregation of mutant AChR in muscle cells ( Borges and Ferns, 2001 ). (jneurosci.org)
The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. (origene.com)
Unique CD18 mutations involving a deletion in the extracellular stalk region and a major truncation of the cytoplasmic domain in a patient with leukocyte adhesion deficiency type 1. (lu.se)

This was something that had not been seen before, which shows the advantage of editing a gene's regulatory sequence at its normal location within the genome. (elifesciences.org)

Population-based Sanger sequencing has been shown to miss minor variants with a frequency below 20% 20 . (nature.com)

The most common mutation results in the replacement of the protein building block (amino acid) arginine with the amino acid tryptophan at position 88 in the septin-9 protein sequence, written as Arg88Trp or R88W. (medlineplus.gov)
The histidine residue is the most common mutation site in Wilson disease, and this histidine residue is essential for the function of the Menkes ATPase, ATP7A. (medscape.com)

They include two different frameshifts, one inframe deletion and one missense mutation. (bmj.com)
A novel 3' splice-site mutation and a novel gross deletion in leukocyte adhesion deficiency (LAD)-1. (lu.se)
Other sources of experimental information include X-ray diffraction, biotinylation, sequence mutation and deletion, solution binding and chemical cross-linking data. (lu.se)
The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)

The ENCODE Project has found that 80 per cent of the human genome sequence is linked to biological function. (blogspot.com)

Using structure-guided sequence analysis we uncover previously uncharacterized CIF peptides conserved among higher plants. (unige.ch)

The sequence contains a conserved tyrosine (Y390) whose phosphorylation is induced by agrin and whose mutation abolished clustering of β loop chimeras and their ability to inhibit agrin-induced clustering of the endogenous AChR. (jneurosci.org)
Mutations in Brutons tyrosine kinase (Btk) within its PH domain cause X-linked agammaglobulinaemia (XLA) in patients. (embl.de)

Intergenerational DNA transmission is shaped by the fundamental processes of chromosome segregation, recombination, and de novo mutation. (jax.org)
The PAR is a region of conserved sequence identity between the X and Y chromosomes over which the meiotic program of pairing, synapsis, and recombination unfolds to ensure correct sex chromosome segregation. (jax.org)
My research aims to understand the causes and consequences of variation in the mechanisms that govern DNA inheritance: chromosome segregation, recombination, and de novo mutation. (jax.org)
ITGB2 mutation combined with deleted ring 21 chromosome in a child with leukocyte adhesion deficiency. (lu.se)

The previously reported pathogenic mutation in NLRP1 in humans, and the missense mutation we inserted into the BALB/c mice in this study. (frontiersin.org)

Clinical, neuroimaging and biochemical findings in patients and patient fibroblasts expressing ten novel GFM1 mutations. (nih.gov)
In our previous study using RT-PCR products, we identified 14 different mutations in 19 alleles from 11 patients, and failed to detect mutations in the remaining three alleles. (bmj.com)
Reversion mutations in patients with leukocyte adhesion deficiency type-1 (LAD-1). (lu.se)
Germline RB1 Mutation in Retinoblastoma Patients: Detection Methods and Implication in Tumor Focality. (cdc.gov)
Follow-up of intraocular retinoblastoma through the quantitative analysis of conserved nuclear DNA sequences in aqueous humor from patients. (cdc.gov)

This mutation was not present in 294 control alleles. (molvis.org)

Vaccines using conserved components of influenza A virus can induce protection against many influenza A strains, including those of divergent subtypes. (cdc.gov)
Our work provides a mechanistic framework for the recognition of sequence-divergent peptide hormones in plants. (unige.ch)

Therefore, Monoclonal Antibodies produced against these targets will expectedly be universal, durable, broadly neutralizing, and unaffected by virus mutation. (bignewsnetwork.com)
As with all of the significant discoveries made by the Company, Enzolytics is preparing patent applications to cover the identified neutralizable, immutable sequences and their use in the creation of anti-Monkeypox Monoclonal Antibodies, vaccines, and diagnostic tests. (bignewsnetwork.com)

A highly conserved sequence is one that has remained relatively unchanged far back up the phylogenetic tree, and hence far back in geological time. (wikipedia.org)
Examples of highly conserved sequences include the RNA components of ribosomes present in all domains of life, the homeobox sequences widespread amongst eukaryotes, and the tmRNA in bacteria. (wikipedia.org)
Highly conserved DNA sequences are thought to have functional value, although the role for many highly conserved non-coding DNA sequences is poorly understood. (wikipedia.org)
Within a sequence, amino acids that are important for folding, structural stability, or that form a binding site may be more highly conserved. (wikipedia.org)
each contains a highly conserved transcription control sequence, the paired box. (nih.gov)
PURA encodes Pur-α, a highly conserved multifunctional protein that has an important role in normal postnatal brain development in animal models. (bmj.com)
The results of this study suggest that some infected cells may produce a highly diverse population, while in others a more conserved sequence is maintained. (virology.ws)
A helix initiation motif and a helix termination motif segment can be found at either end of the alpha-helical rod and are highly conserved in sequence between keratins. (medscape.com)
As with most other keratin disorders, most mutations in pachyonychia congenita occur in these highly conserved helix boundary domains at the end of the rod domain. (medscape.com)
Highly conserved regions are less likely to mutate, therefore there is a high likelihood that the diagnostic test will remain accurate in the presence of variants. (who.int)
The 16S rRNA gene is highly conserved with certain variable regions. (folkhalsomyndigheten.se)

Changes in the SEPTIN9 gene may alter the sequence of amino acids in certain septin-9 isoforms in ways that interfere with their function. (medlineplus.gov)
Of 320 amino acids of AtNAS1, twelve, 287-TRGCMFMPCNCS-298, accounted for the autoinhibitory effect of the C terminus, of which approximately one-third was attributed to N296 within a CNCS motif that is fully conserved in Arabidopsis. (bvsalud.org)

Currently, manufacturers are focusing on and targeting assays to mutations in the S gene. (who.int)

The study of sequence conservation overlaps with the fields of genomics, proteomics, evolutionary biology, phylogenetics, bioinformatics and mathematics. (wikipedia.org)

Isolates from this patient, sequenced by the state laboratory, demonstrated genotypic changes in F13L associated with tecovirimat resistance. (cdc.gov)

Mutations in a related gene, Pax-6, which, like Pax-3, has both a paired box and a paired-type homeobox sequence, cause the Small-eye mutation in mice and aniridia in man. (nih.gov)

A number of tools are available for the detection of mutations that are characteristic of viruses defined as VOI/VOCs. (who.int)
Each variant will have characteristic mutations or a group of mutations that is/are unique to that particular variant. (who.int)

The laboratory procedure is based on amplifying and sequencing four variable regions of the 16S rRNA gene with two amplicons. (folkhalsomyndigheten.se)
The procedure's high sequence depth allows for these amplicons to be analysed separetely so different bacteria from the same sample can be identified. (folkhalsomyndigheten.se)

The receptor of SARS-CoV-2, i.e., angiotensin I converting enzyme 2 (ACE2) is conserved in pangolins ( 18 ) and coronaviruses isolated from pangolins have a receptor binding domain in their spike protein that is uniquely similar to that of SARS-CoV-2 ( 10 , 19 ). (frontiersin.org)
This amino acid change appears to increase the mutation rate of the enzyme. (virology.ws)
Chemical modification of the histidines using DEPC or by site-directed mutations resulted in partial or total inactivation of the enzyme. (lu.se)

Conserved sequences are typically identified by bioinformatics approaches based on sequence alignment. (wikipedia.org)

In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)

For each infected cell, 7-10 plaques were picked and used for massive parallel genome sequencing. (virology.ws)

The objective of this document is to outline the available methods and tools for screening and sequencing patient specimens for the detection of VOC or VOI, with a focus on those that offer a rapid turn-around time so as to support rapid implementation of public health actions for COVID 19 control. (who.int)
CDC has analyzed sequences from more than 4,000 specimens from across the world, and only 13 changes in the F13L protein were found, including the two cases included in this HAN Update. (cdc.gov)

The method uses an internal control sequence (spike) which is amplified and sequenced simultaneously with the sample to facilitate the assessment of the bacterial content and to detect amplification-inhibiting factors in the sample that may lead to a negative result. (folkhalsomyndigheten.se)

Sequencing of the 3′ and 5′ termini of the BUNdelNSs S RNA segment and analysis using a minireplicon system show that the N overexpressing phenotype results from a single nucleotide substitution at position 16 in the 3′ terminus. (microbiologyresearch.org)
We performed pathogenicity analysis on the mutation and selected a suitable assisted reproductive technology solution for the patient. (researchsquare.com)
The result of of metagenomic sequencing is the outcome of a collaborative effort between bioinformaticians and clinical microbiologists, where the clinical interpretation confirms and complements the technical analysis. (folkhalsomyndigheten.se)
Sequence variations between different bacteria provide a unique identity, and the analysis of these sequence enables taxonomic placement. (folkhalsomyndigheten.se)

We are also interested in understanding how oligomerization specificity is encoded in protein sequence and structure, in particular in coiled-coils. (lu.se)

We studied DNA vaccination in mice with plasmids expressing conserved nucleoprotein (NP) and matrix (M) from an H1N1 virus. (cdc.gov)

The Company's Artificial Intelligence platform has also been used to build AI-generated 3D Models of all the conserved targets. (bignewsnetwork.com)
Background Neoantigens, new immunogenic sequences arising from tumor mutations, have been associated with response to immunotherapy and are considered potential targets for vaccination. (unav.edu)

article{939b7e14-4ce7-4517-a0a3-5bfc01c5e6af, abstract = {{The violaxanthin de-epoxidase (VDE) gene from spinach (Spinacia oleracea) was cloned, sequenced (GenBank AJ 250433), and expressed in Escherichia coli. (lu.se)

Metagenomic sequencing is offered in cases where infection is suspected but other methods have failed to identify a pathogen. (folkhalsomyndigheten.se)

This mutation could also be detected in wtBUN populations, and was isolated by plaquing a 'wt' variant carrying the mutation. (microbiologyresearch.org)

Glucose and GLP-1 also synergistically activated NFAT (nuclear factor of activated T-cells)-mediated transcription from a minimal promoter construct containing tandem NFAT consensus sequences. (diabetesjournals.org)

Anatomy11

Organisms12

Diseases23

Chemicals and Drugs74

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Psychiatry and Psychology2

Phenomena and Processes111

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MRNA3

Multiple sequence alignments1

Monkeypox Virus2

Homology1

Species6

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Nucleic acid3

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Heterozygous2

Transcription factors2

Functional sequences2

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Human genome1

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