• enzyme
  • In this work we study the expression of RNase R in the human pathogen Streptococcus pneumoniae and analyse the interplay of this enzyme with the main components of the trans -translation machinery (SmpB and tmRNA/SsrA). (biomedcentral.com)
  • Frederic Middlebrook Richards (August 19, 1925 - January 11, 2009), commonly referred to as Fred Richards, was an American biochemist and biophysicist known for solving the pioneering crystal structure of the ribonuclease S enzyme in 1967 and for defining the concept of solvent-accessible surface. (wikipedia.org)
  • In 1954 he went to the Carlsberg Laboratory in Copenhagen to do postdoctoral research with Kaj Linderstrøm-Lang, where he started his classic work on the ribonuclease enzyme. (wikipedia.org)
  • RNase L is an enzyme that in humans is encoded by the RNASEL gene. (wikipedia.org)
  • Ribonuclease E (EC 3.1.26.12, endoribonuclease E, RNase E, Rne protein) is an enzyme. (wikipedia.org)
  • This enzyme catalyses the following chemical reaction Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions RNase E is a bacterial ribonuclease that participates in the processing of ribosomal RNA (9S to 5S rRNA) and the chemical degradation of bulk cellular RNA. (wikipedia.org)
  • RNase MRP also demonstrated cleavage ability of the 5′-UTR of CLB2 mRNA that allows for rapid 5′-to-3′ degradation by XRN1, an exoribonuclease enzyme. (wikipedia.org)
  • 2012, it was made targeted mutagenesis of the mouse Rnaseh2b gene to gain insight into the in vivo role of the mammalian RNase H2 enzyme, because the ablation of Rnaseh2b in mice leads to early embryonic lethality. (wikipedia.org)
  • 2. RNase H2 is a genome surveillance enzyme required for ribonucleotide removal: They found that ribonucleotide accumulation in genomic DNA of RNaseH2null mice implicates the RNaseH2 complex in the maintenance of genome integrity. (wikipedia.org)
  • cleave
  • RNase MRP has been shown to cleave an internal transcribed spacer, specifically ITS1 at the specific site A3 of the rRNA precursor, leading, after additional trimming, to the formation of the mature 5′-end of 5.8S rRNA. (wikipedia.org)
  • Instead, RDE-1 with mutations in the conserved DDH motif exhibit reduced passenger (sense) strand turnover, suggesting that RNase H activity serves to cleave the passenger strand, leaving the guide (antisense) strand accessible for base-pairing to target mRNA. (wikipedia.org)
  • inactivation
  • The present invention relates to attenuated pestiviruses, in particular to attenuated BVDV, wherein at least one mutation is in the coding sequence for glycoprotein Erns and at least another mutation in the coding sequence for Npro which preferably leads to combined inactivation of the RNase activity residing in glycoprotein Erns in addition to the inactivation of the (hypothesized) immune-modulating activity residing in Npro. (allindianpatents.com)
  • Recent data that has been gathered using several temperature-sensitive RNase MRP mutants that showed that inactivation of RNase MRP leading to severe reduction of the abundance of all early intermediates in the typical rRNA processing pathway. (wikipedia.org)
  • sequences
  • Sequences of the CR-I, CR-V, and CR-IV genes in domain 1 of the P4 helical region are conserved, with the consensus sequence in CR-IV being AGNNNNA for RNAse P and AGNNA for RNase MRP. (wikipedia.org)
  • It is generally noted that overall variation in the expression of affected genes and the number of repeat sequences of the RNA have little to do with the pathogenic consequences, but rather it is the gain of organizational and catalytic functions of the mutant RNA, which is similar to that of mutant proteins, that causes the toxicity. (wikipedia.org)
  • In the study, the gapmers tested contained specific modified nucleic acids which target Ribonuclease and prompted H-mediated cleaving of intended RNA sequences. (wikipedia.org)
  • gene
  • On the other hand, our results revealed a strong accumulation of the smpB transcript in the absence of RNase R at 15°C. Transcriptional analysis of the S. pneumoniae rnr gene demonstrated that it is co-transcribed with the flanking genes, secG and smpB . (biomedcentral.com)
  • Responsible for this disease is a mutation in the RNase MRP RNA gene (RMRP), a non-coding RNA gene. (wikipedia.org)
  • Metaphyseal dysplasia without hypotrichosis (MDWH), anauxetic dysplasia (AD), kyphomelic dysplasia (KD), Omenn syndrome (OS) are diseases associated with mutated and (or) dysfunctional RNAse MRP activity, hence, the RMRP gene. (wikipedia.org)
  • The first type is when an insertion, duplication, or triplication occurs at the promoter of the RNAse MRP gene between the TATA box and the transcription initiation site. (wikipedia.org)
  • Patients with CHH have been identified to have over 70 different mutations in the RNA transcript made by RNAse MRP, whereas around 30 distinct mutations have been identified in the promoter region of the RNAse MRP gene. (wikipedia.org)
  • The rde-1 gene locus was first characterized in C. elegans mutants resistant to RNAi, and is a member of a highly conserved Piwi gene family that includes plant, Drosophila, and vertebrate homologs. (wikipedia.org)
  • rRNA
  • However, the transcription of the rRNA precursor is not affected, thus suggesting that RNase MRP plays a key role in the processing of rRNA beyond the cleavage of the A3 site in ITS1. (wikipedia.org)
  • Additionally
  • Additionally recombination with an albino mutant led to the production of heterokaryotic conidial heads with mixed conidial colours. (wikipedia.org)
  • single-stranded
  • Further, target silencing can be fully restored in DDH motif mutants by loading single-stranded siRNA, suggesting that a downstream component in the RNAi pathway is responsible for Slicer activity. (wikipedia.org)
  • fragments
  • The RNA fragments produced by RNAse L have double stranded regions, as well as specific markers, that allow them to be identified by the RNAse L and MDA5. (wikipedia.org)
  • mutations
  • Human ribonuclease H2 is a heterotrimeric complex composed of three subunits, mutations in any of which are among the genetic causes of a rare disease known as Aicardi-Goutières syndrome. (wikipedia.org)
  • Mutations in the RNA component of RNase MRP cause cartilage-hair hypoplasia, a pleiotropic human disease. (wikipedia.org)
  • Two categories of mutations involving RNAse MRP have been identified in patients with CHH. (wikipedia.org)
  • The second category consists of mutations that are in the transcribed RNA made by the RNAse MRP. (wikipedia.org)
  • activity
  • Although an RNase H domain was tentatively identified in the retron ORF, it was later found that the RNase H activity required for msDNA synthesis is actually supplied by the host. (wikipedia.org)
  • In a cell with RNAse L, MDA5 activity may be further enhanced. (wikipedia.org)
  • Some studies have suggested that high levels of RNAse L may actually inhibit IFN-β production, but a clear linkage still exists between RNAse L activity and IFN-β production. (wikipedia.org)
  • Unlike in other Argonautes characterized in Drosophila and humans, the catalytic RNase H motif in RDE-1 has not been shown to exhibit Slicer activity of the target transcript. (wikipedia.org)
  • molecules
  • Cold-shock treatment is a condition which thermodynamically favours the formation of highly structured RNA molecules, and this fact probably leads to the marked increase of RNase R under this stress situation. (biomedcentral.com)
  • 2-5 A molecules then bind to RNase L, promoting its activation by dimerization. (wikipedia.org)
  • In its activated form RNase L cleaves all RNA molecules in the cell leading to autophagy and apoptosis. (wikipedia.org)
  • Upon the addition of 2-5A molecules, RNase L becomes active, cleaving the oligoribonucleotides and interfering in the FRET signal. (wikipedia.org)
  • inactive
  • This dimerization and activation of RNase L can be recognized using Fluorescence Resonance Energy Transfer (FRET), as oligoribonucleotides containing a quencher and a fluorophore on opposite sites are added to a solution with inactive RNase L. The FRET signal is then recorded as the quencher and the fluorophore are very close to each other. (wikipedia.org)
  • Interactions
  • The data presented here shed light on the interactions between RNase R, trans -translation and cold-shock response in an important human pathogen. (biomedcentral.com)
  • amino
  • Within Malus , the S kb-RNase from M. × domestica and S t-RNase from M. transitoria show 100% identity of the HV region at the deduced amino acid level, suggesting that these S -RNases diverged more recently than the other Malus S -RNases. (springer.com)
  • consists
  • The structure of RNase H commonly consists of a 5-stranded β-sheet surrounded by a distribution of α-helices. (wikipedia.org)
  • known
  • There are currently no established treatments, but, one study, focused on myotonic dystrophy type 1, suggests that the use of antisense oligonucleotides (ASOs) known as gapmers can aid in decreasing the mutant RNA repeat transcripts. (wikipedia.org)
  • present
  • RNase L is present in very minute quantities during the normal cell cycle. (wikipedia.org)
  • The fact that there is not often a mutation in the promoter region in both alleles shows the lethality of not having this RNA present that is transcribed by RNAse MRP. (wikipedia.org)