• mutations
  • We report a sensitive method to detect point mutations in proteins from complex samples. (uva.nl)
  • Taken together, our data demonstrate that truncated proteins arising from BRCA1 185delAG mutation increase Akt-mediated apoptosis by increasing nuclear maspin expression, suggesting a possible mechanism by which ovarian cancer patients with germline BRCA1 mutations may respond better to chemotherapy. (usf.edu)
  • Mutant Proteins and Protein Evolution (starts 4:42) CU School of Medicine professor David Pollock explains why he has devised a new way to identify and predict both the evolution of proteins and disease causing protein mutations. (howonearthradio.org)
  • However, its major drawback is that detailed structural knowledge of a protein is often unavailable, and, even when available, it can be very difficult to predict the effects of various mutations. (wikipedia.org)
  • peptides
  • However, other methodologies would potentially provide additional information such as studies on the effects of Cry proteins and derived peptides on the indigenous gastrointestinal microbiota and on intestinal epithelial cells of humans. (wur.nl)
  • As there is growing evidence indicating that the more common neurodegenerative diseases, notably Alzheimer's and Parkinson's diseases, are also caused by aggregation of peptides or proteins such as Aβ, tau, and α-synuclein, it is possible that this assay could be adopted for clinical application across a spectrum of neurodegenerative disorders. (sciencemag.org)
  • D, 13C, or 15N) peptides to a complex matrix as concentration standards, SRM can be used to construct a calibration curve that can provide the absolute quantification (i.e., copy number per cell) of the native, light peptide, and by extension, its parent protein. (wikipedia.org)
  • S100
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (wikipedia.org)
  • molecular
  • Two types of DNA damage that frequently befall most cells on an everyday basis can lead to the creation of damaged proteins that may contribute to neurodegeneration, aging and cancer, according to research by scientists at Emory University School of Medicine, published in the October 23 issue of the journal Molecular Cell. (innovations-report.com)
  • Our results indicate that distinct molecular properties exist among the wild-type and dystrophy-related mutant BIGH3 proteins. (arvojournals.org)
  • designed to investigate
  • The study, led by Professor Dr. Adriano Aguzzi of the Institute of Neuropathology at the University of Zurich in Switzerland, was designed to investigate the specific changes in the prion protein that may contribute to chronic wasting disease (CWD). (bio-medicine.org)
  • stabilization
  • The present study was undertaken to identify the mini-chaperone interacting site(s) during the stabilization of the mutant αA-G98R protein. (arvojournals.org)
  • Deletion of the co-chaperone AHA1 (activator of heat shock 90kDa protein ATPase homolog 1) leads to stabilization of CFTR and opens up a perspective for a new therapy. (wikipedia.org)
  • 2002
  • Bryant DA, Vassilieva EV, Frigaard NU, Li H (2002) Selective protein extraction from Chlorobium tepidum chlorosomes using detergents: Evidence that CsmA forms multimers and binds bacteriochlorophyll a . (springer.com)
  • membrane
  • Self-aggregated bacteriochlorophyll pigments are surrounded by a monolayer envelope membrane comprised of glycolipids and Csm proteins. (springer.com)
  • This protein is cytoplasmic but is often anchored to a peroxisomal membrane where it forms a heteromeric complex and plays a role in the import of proteins into peroxisomes and peroxisome biogenesis. (wikipedia.org)
  • TOM70 (translocase of the outer mitochondrial membrane of ~70 kDa) mediates translocation of client proteins through the import pore into the mitochondrial matrix. (wikipedia.org)
  • eukaryotic
  • The unusual amino acid hypusine is formed posttranslationally and is only found in a single cellular protein, eukaryotic translation initiation factor 5A. (wikipedia.org)
  • wild-type
  • High-resolution crystallographic refinement of the mutant lysozyme structure confirms that it is very similar to wild-type lysozyme. (rcsb.org)
  • BRCA1 wild-type IOSE cells were transfected with BRAt protein and showed increased maspin mRNA levels and increased nuclear maspin protein levels as compared to control cells. (usf.edu)
  • 2. The mutant protein according to claim 1, wherein the mutant protein has a modified conformation such that hydrophobicity in the vicinity of a coenzyme-binding site is higher than that of a wild-type protein. (patentsencyclopedia.com)
  • The ratio of monogalactosyldiacylglyceride (MGDG) to rhamnosylgalactosyldiacylglyceride (RGDG) was smaller in chlorosomes from mutants lacking two or three proteins in CsmC/D/H motif family than in chlorosomes from the wild-type, whereas chlorosomes lacking CsmIJ showed relatively less RGDG than MGDG. (springer.com)
  • Thioflavin T (Tht) and Congo red were also used to compare the fibril formation among wild-type and mutant proteins in vitro . (arvojournals.org)
  • substitutions
  • Functional Apolipoprotein A-I mutant proteins, having one or more cysteine substitutions and polynucleotides encoding same, can be used to modulate paraoxonase's arylesterase activity. (energy.gov)
  • partially
  • A water molecule bound in the vicinity of Thr155 partially restores the hydrogen bond network in the mutant structure, but the buried main-chain amide of Asp159 is not near a hydrogen bond acceptor. (rcsb.org)
  • When imidazole buffer is added to cells expressing mutant proteins, the ion selectivity is partially rescued. (wikipedia.org)
  • thereby
  • The results suggest that the CsmC, CsmD, CsmH, and other chlorosome proteins are involved in organizing MGDG and RGDG and thereby affect the size and shape of the chlorosome. (springer.com)
  • Chaperones are proteins that bind to other proteins, thereby stabilizing them in an ATP-dependent manner. (wikipedia.org)
  • transgenic
  • Nevertheless, there is some concern in relation to the food safety of transgenic crops, especially to the heterologously expressed proteins. (wur.nl)
  • demonstrate
  • The researchers went on to demonstrate that the reduction in methylation of normal H3 histones results from interference with activity of a methyltransferase called PRC2 by the mutant histone. (redorbit.com)
  • therapeutic
  • These ApoA-I mutant proteins can be used as therapeutic agents to combat cardiovascular disease, atherosclerosis, acute phase response and other inflammatory related diseases. (energy.gov)
  • nuclear
  • Proliferating cell nuclear antigen (PCNA) is a versatile protein involved in all pathways of DNA metabolism. (uiowa.edu)
  • regulates
  • Rubinsztein has made major contributions to the field of neurodegeneration with his laboratory's discovery that autophagy regulates the levels of intracytoplasmic aggregate-prone proteins that cause many neurodegenerative diseases, including Huntington's, Parkinson's and Alzheimer's disease. (wikipedia.org)
  • stabilizes
  • For example, the co-chaperone CDC37 (cell division cycle 37) stabilizes the cell cycle regulatory proteins CDK4 (cyclin dependent kinase 4) and Cdk6. (wikipedia.org)
  • motif
  • To facilitate protein purification, a (His) 6 -tag motif was inserted after the sequence encoding the signal peptide of BIGH3 in expression plasmids and a strep-tag II motif was inserted before the RGD sequence in the C-terminus. (arvojournals.org)
  • activity
  • The investigators used e. coli cells as a model system to study specific kinds of genetic damages that occur in all non-dividing cells undergoing transcription -- the everyday activity in which cells produce the proteins necessary to carry out bodily processes. (innovations-report.com)
  • Among them, Cry8Ka5 mutant protein showed coleoptericidal activity 3-fold higher (LC50 2.83 μg/mL) than that of the original protein (Cry8Ka1). (wur.nl)
  • 4. The mutant protein according to claim 3, wherein the residual activity is 27% or more after heating. (patentsencyclopedia.com)
  • Those affected with CARASIL display mutant proteins unable to suppress TGF-β activity. (wikipedia.org)
  • cell
  • In some cases this miscoding could cause a cell to manufacture a mutant protein that controls cell division, which could take the cell from a non-growth state to a growth state and contribute to malignant transformation in the case of mammalian cells. (innovations-report.com)
  • Two mutant forms of yeast PCNA, one with an E113G substitution and one with a G178S substitution, support normal cell growth but inhibit TLS. (uiowa.edu)
  • report the development of a cell-based assay that can accurately identify mutant huntingtin protein in the cerebrospinal fluid of HD patients. (sciencemag.org)
  • In cell biology, a biomarker is a molecule that allows the detection and isolation of a particular cell type (for example, the protein Oct-4 is used as a biomarker to identify embryonic stem cells). (wikipedia.org)
  • 1998). "Human PEX1 cloned by functional complementation on a CHO cell mutant is responsible for peroxisome-deficient Zellweger syndrome of complementation group I". Proc. (wikipedia.org)
  • advances
  • In the future, more detailed knowledge of protein structure and function, and advances in high-throughput screening, may greatly expand the abilities of protein engineering. (wikipedia.org)
  • transcription
  • TM occurs when cells with damaged DNA produce bad messages during transcription that lead to the creation of mutant proteins. (innovations-report.com)
  • One type of spontaneous genetic damage occurs in non-dividing cells when cytosine (C), one of the four amino-acid bases (A, T, G, and C) spontaneously changes to uracil (U). This common substitution causes genetic miscoding that can lead to TM and the manufacture of mutant proteins during transcription. (innovations-report.com)
  • amyloid
  • Prion diseases are thought to be a result of a misfolded form of the prion protein that induces formation of amyloid plaques in the brain changes that are also seen in patients with Alzheimer's disease. (bio-medicine.org)
  • It could be that this 'loop' region of the protein can promote the formation of amyloid plaques in the brain," said Sigurdson. (bio-medicine.org)
  • recognition
  • During MMR, PCNA recruits and coordinates proteins involved in the mismatch recognition, excision, and resynthesis steps. (uiowa.edu)
  • It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles. (wikipedia.org)
  • Previously
  • Previously, two mutant forms of PCNA were identified that cause defects in MMR with little if any other defects. (uiowa.edu)
  • functional
  • Frigaard NU, Li H, Milks KJ, Bryant DA (2004) Nine mutants of Chlorobium tepidum each unable to synthesize a different chlorosome protein still assemble functional chlorosomes. (springer.com)
  • genetic
  • Although our study was in e.coli, very similar systems operate to repair genetic damage in human cells, thus this is a very important model for helping understand the mechanisms in non-dividing cells that can cause the manufacture of mutant proteins as a result of genetic damage to cells, says Dr. Doetsch. (innovations-report.com)
  • cells
  • Most of the cells within organisms are no longer replicating, however, and instead spend their time manufacturing proteins. (innovations-report.com)
  • The second part of the hypothetical situation is that once the mutant line has been isolated after a period of time mononucleate cells begin to appears. (biology-online.org)
  • Cells transfected with the BRCA1 185delAG truncation protein (BRAt) showed increased levels of active caspase 3, increased cleavage of caspase 3 substrates, PARP and DFF45, and decreased XIAP and cIAP1 following staurosporine (STS) treatment. (usf.edu)
  • Similarly, maspin protein has been shown to sensitize breast carcinoma cells to STS-induced apoptosis. (usf.edu)
  • We provide the first evidence that BRAt is sufficient to induce maspin protein in IOSE cells. (usf.edu)
  • The transduced mutant αA-G98R protein forms larger aggregates in HLE-B3 cells compared to the αA-Wt. (arvojournals.org)
  • They also found a global reduction in the levels of methylation of normal H3 histones when small amounts of the mutant H3 were added to normal human cells. (redorbit.com)
  • cancer
  • Their findings, published last week in Science Express, follow a recent series of discoveries by several international genome sequencing consortiums that directly links a mutated histone protein to a rare brain stem cancer in children called DIPG. (redorbit.com)
  • identify
  • Computational protein design algorithms seek to identify novel amino acid sequences that are low in energy when folded to the pre-specified target structure. (wikipedia.org)