• mutations
  • Taken together, our data demonstrate that truncated proteins arising from BRCA1 185delAG mutation increase Akt-mediated apoptosis by increasing nuclear maspin expression, suggesting a possible mechanism by which ovarian cancer patients with germline BRCA1 mutations may respond better to chemotherapy. (usf.edu)
  • Mutant Proteins and Protein Evolution (starts 4:42) CU School of Medicine professor David Pollock explains why he has devised a new way to identify and predict both the evolution of proteins and disease causing protein mutations. (howonearthradio.org)
  • However, its major drawback is that detailed structural knowledge of a protein is often unavailable, and, even when available, it can be very difficult to predict the effects of various mutations. (wikipedia.org)
  • S100
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (wikipedia.org)
  • aggregation
  • As there is growing evidence indicating that the more common neurodegenerative diseases, notably Alzheimer's and Parkinson's diseases, are also caused by aggregation of peptides or proteins such as Aβ, tau, and α-synuclein, it is possible that this assay could be adopted for clinical application across a spectrum of neurodegenerative disorders. (sciencemag.org)
  • Huntington's disease cerebrospinal fluid seeds aggregation of mutant huntingtin. (sciencemag.org)
  • designed to investigate
  • The study, led by Professor Dr. Adriano Aguzzi of the Institute of Neuropathology at the University of Zurich in Switzerland, was designed to investigate the specific changes in the prion protein that may contribute to chronic wasting disease (CWD). (bio-medicine.org)
  • eukaryotic
  • The unusual amino acid hypusine is formed posttranslationally and is only found in a single cellular protein, eukaryotic translation initiation factor 5A. (wikipedia.org)
  • substitutions
  • Functional Apolipoprotein A-I mutant proteins, having one or more cysteine substitutions and polynucleotides encoding same, can be used to modulate paraoxonase's arylesterase activity. (energy.gov)
  • survival
  • After creating a new mouse model of SBMA, they discovered that skeletal muscle was the site of mutant protein toxicity and that measures which mitigated the protein's influence in muscle suppressed symptoms of SBMA in treated mice, such as weight loss and progressive weakness, and increased survival. (blogspot.com)
  • They are able to stabilize mutant proteins thereby allowing survival and increased proliferation of cancer cells. (wikipedia.org)
  • homolog
  • The M2 protein of influenza B is 109 residue long, homo tetramer and is a functional homolog of influenza A protein. (wikipedia.org)
  • Deletion of the co-chaperone AHA1 (activator of heat shock 90kDa protein ATPase homolog 1) leads to stabilization of CFTR and opens up a perspective for a new therapy. (wikipedia.org)
  • demonstrate
  • The researchers went on to demonstrate that the reduction in methylation of normal H3 histones results from interference with activity of a methyltransferase called PRC2 by the mutant histone. (redorbit.com)
  • target protein
  • These techniques involve alignment of target protein sequences with other related protein sequences. (wikipedia.org)
  • Multiple sequence alignment utilizes data bases such as PREFAB, SABMARK, OXBENCH, IRMBASE, and BALIBASE in order to cross reference target protein sequences with known sequences. (wikipedia.org)
  • nuclear
  • Proliferating cell nuclear antigen (PCNA) is a versatile protein involved in all pathways of DNA metabolism. (uiowa.edu)
  • BRCA1 wild-type IOSE cells were transfected with BRAt protein and showed increased maspin mRNA levels and increased nuclear maspin protein levels as compared to control cells. (usf.edu)
  • Previously
  • Previously, two mutant forms of PCNA were identified that cause defects in MMR with little if any other defects. (uiowa.edu)
  • regulates
  • Rubinsztein has made major contributions to the field of neurodegeneration with his laboratory's discovery that autophagy regulates the levels of intracytoplasmic aggregate-prone proteins that cause many neurodegenerative diseases, including Huntington's, Parkinson's and Alzheimer's disease. (wikipedia.org)
  • cell
  • In some cases this miscoding could cause a cell to manufacture a mutant protein that controls cell division, which could take the cell from a non-growth state to a growth state and contribute to malignant transformation in the case of mammalian cells. (innovations-report.com)
  • Two mutant forms of yeast PCNA, one with an E113G substitution and one with a G178S substitution, support normal cell growth but inhibit TLS. (uiowa.edu)
  • report the development of a cell-based assay that can accurately identify mutant huntingtin protein in the cerebrospinal fluid of HD patients. (sciencemag.org)
  • D, 13C, or 15N) peptides to a complex matrix as concentration standards, SRM can be used to construct a calibration curve that can provide the absolute quantification (i.e., copy number per cell) of the native, light peptide, and by extension, its parent protein. (wikipedia.org)
  • In cell biology, a biomarker is a molecule that allows the detection and isolation of a particular cell type (for example, the protein Oct-4 is used as a biomarker to identify embryonic stem cells). (wikipedia.org)
  • 1998). "Human PEX1 cloned by functional complementation on a CHO cell mutant is responsible for peroxisome-deficient Zellweger syndrome of complementation group I". Proc. (wikipedia.org)
  • For example, the co-chaperone CDC37 (cell division cycle 37) stabilizes the cell cycle regulatory proteins CDK4 (cyclin dependent kinase 4) and Cdk6. (wikipedia.org)
  • sequence
  • The invention also includes modifications and optimizations of the ApoA-I nucleotide sequence for purposes of increasing protein expression and optimization. (energy.gov)
  • A mutant protein includes an amino acid sequence obtained by deletion, replacement, addition, or insertion of at least one amino acid residue of a native-form amino acid sequence of SEQ. (patentsencyclopedia.com)
  • 11. The mutant protein according to claim 2, wherein the mutant protein is one protein selected from(a) a protein having an amino acid sequence of SEQ. (patentsencyclopedia.com)
  • To facilitate protein purification, a (His) 6 -tag motif was inserted after the sequence encoding the signal peptide of BIGH3 in expression plasmids and a strep-tag II motif was inserted before the RGD sequence in the C-terminus. (arvojournals.org)
  • While the sequence-conformation space that needs to be searched is large, the most challenging requirement for computational protein design is a fast, yet accurate, energy function that can distinguish optimal sequences from similar suboptimal ones. (wikipedia.org)
  • advances
  • In the future, more detailed knowledge of protein structure and function, and advances in high-throughput screening, may greatly expand the abilities of protein engineering. (wikipedia.org)
  • transcription
  • The investigators used e. coli cells as a model system to study specific kinds of genetic damages that occur in all non-dividing cells undergoing transcription -- the everyday activity in which cells produce the proteins necessary to carry out bodily processes. (innovations-report.com)
  • TM occurs when cells with damaged DNA produce bad messages during transcription that lead to the creation of mutant proteins. (innovations-report.com)
  • One type of spontaneous genetic damage occurs in non-dividing cells when cytosine (C), one of the four amino-acid bases (A, T, G, and C) spontaneously changes to uracil (U). This common substitution causes genetic miscoding that can lead to TM and the manufacture of mutant proteins during transcription. (innovations-report.com)
  • coli
  • Although our study was in e.coli, very similar systems operate to repair genetic damage in human cells, thus this is a very important model for helping understand the mechanisms in non-dividing cells that can cause the manufacture of mutant proteins as a result of genetic damage to cells, says Dr. Doetsch. (innovations-report.com)
  • Beachy, R. N. 2004-04-01 00:00:00 The assembly of Tobacco etch potyvirus (TEV) coat protein (CP) and truncated mutants in Escherichia coli was studied. (deepdyve.com)
  • However, while the wild type and the Arg154Gln mutant CP were each able to form PVLPs in E. coli , the Asp198Glu and the double mutant Ser152Pro/Arg154Gln mutants did not. (deepdyve.com)
  • amyloid
  • Prion diseases are thought to be a result of a misfolded form of the prion protein that induces formation of amyloid plaques in the brain changes that are also seen in patients with Alzheimer's disease. (bio-medicine.org)
  • It could be that this 'loop' region of the protein can promote the formation of amyloid plaques in the brain," said Sigurdson. (bio-medicine.org)
  • recognition
  • During MMR, PCNA recruits and coordinates proteins involved in the mismatch recognition, excision, and resynthesis steps. (uiowa.edu)
  • It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles. (wikipedia.org)
  • cancer
  • Their findings, published last week in Science Express, follow a recent series of discoveries by several international genome sequencing consortiums that directly links a mutated histone protein to a rare brain stem cancer in children called DIPG. (redorbit.com)
  • identify
  • Computational protein design algorithms seek to identify novel amino acid sequences that are low in energy when folded to the pre-specified target structure. (wikipedia.org)
  • human
  • These changes resulted in a "loop" in the protein structure of the mice that was rigid similar to the structure of the elk prion protein, and unlike the flexible "loop" found in normal mouse or human prion proteins. (bio-medicine.org)
  • They also found a global reduction in the levels of methylation of normal H3 histones when small amounts of the mutant H3 were added to normal human cells. (redorbit.com)
  • cells
  • Most of the cells within organisms are no longer replicating, however, and instead spend their time manufacturing proteins. (innovations-report.com)
  • The second part of the hypothetical situation is that once the mutant line has been isolated after a period of time mononucleate cells begin to appears. (biology-online.org)
  • Cells transfected with the BRCA1 185delAG truncation protein (BRAt) showed increased levels of active caspase 3, increased cleavage of caspase 3 substrates, PARP and DFF45, and decreased XIAP and cIAP1 following staurosporine (STS) treatment. (usf.edu)
  • Similarly, maspin protein has been shown to sensitize breast carcinoma cells to STS-induced apoptosis. (usf.edu)
  • We provide the first evidence that BRAt is sufficient to induce maspin protein in IOSE cells. (usf.edu)
  • model
  • This new mouse model of the disease may be useful in our understanding of how the misfolded protein leads to neurodegeneration and for testing new therapies against prion disease. (bio-medicine.org)
  • We now have a model for the promotion of brain stem cancers through aberrant epigenetic silencing through the inhibition of PRC2 by a mutant histone," says Allis. (redorbit.com)
  • synthase
  • In the first step of hypusine biosynthesis, deoxyhypusine synthase catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. (wikipedia.org)
  • differences
  • The attenuation of Gbp mutant strains was unlikely due to differences in acid-related properties since the mutants were at least as acidogenic and acid-tolerant as the parental strain. (omicsonline.org)
  • however
  • None of the mutants were, however, able to use exogenous ammonia as a substrate. (inria.fr)
  • However, a recent solid state NMR spectroscopy structure shows that the M2 channel has two binding sites for amantadine, one high affinity site is in the N terminal lumen, and a second low affinity site on the C terminal protein surface. (wikipedia.org)
  • Site
  • The mutant forms of PCNA and Ub-PCNA do not stimulate TLS of an abasic site by either classical pol δ or non-classical pol η. (uiowa.edu)