• Analyses of the molecular pathophysiology using the primary cells from the Tmem53 -/- mice and the TMEM53 knock-out cell lines indicates that TMEM53 inhibits BMP signaling in osteoblast lineage cells by blocking cytoplasm-nucleus translocation of BMP2-activated Smad proteins. (nature.com)
  • We show that TMEM53 acts as an inhibitor of BMP-SMAD signaling by preventing SMAD accumulation in the cell nucleus and that its deficiency enhances osteogenic differentiation by overactivating the BMP signaling pathway. (nature.com)
  • This nucleoplasmic lamin A environment tends to be euchromatic, suggesting distinct roles of lamin A in the regulation of gene expression in peripheral and more central regions of the nucleus. (frontiersin.org)
  • LINC complexes interact with lamins in the nucleus and with various cytoplasmic KASH effectors from the surface of nuclei. (ucdavis.edu)
  • The nuclear lamina is a complex network of nuclear lamins and lamin-associated nuclear membrane proteins, which scaffold the nucleus to maintain structural integrity. (bvsalud.org)
  • Epigenetic modifications and the architecture of chromatin in the nucleus, through the formation of specific regions of active as well as inactive chromatin, allow the coordinated regulation of the genes for each cell fate. (bvsalud.org)
  • (D) Nucleoplasmic lamin A interacts with chromatin in the nuclear interior. (frontiersin.org)
  • Impaired spatial and temporal tethering of lamin proteins to the lamina or nucleoplasmic aggregation of lamins are the primary molecular events that can trigger nuclear proteotoxicity by modulating differential protein-protein interactions, sequestering quality control proteins, and initiating a cascade of abnormal post-translational modifications. (bvsalud.org)
  • Fibroblasts extracted from lamin B1 mutant mouse embryos shown early senescence (12). (lavoixdesrroms.org)
  • Radial placement of beads is determined from, here, lamin A ChIP-sequencing data in fibroblasts. (frontiersin.org)
  • Similarly, intercellular transfer of mitochondria-related EVs occurred between A11 cells and α-smooth muscle actin (α-SMA)-positive cancer-associated fibroblasts (CAFs, WA-mFib), macrophages (RAW264.7) and cytotoxic T cells (CTLL-2). (biomedcentral.com)
  • If a cell retains DNA damage, transcription of a gene can be prevented and thus translation into a protein will also be blocked. (wikipedia.org)
  • In this study, we discover a previously unknown type of SBD and identify its causal gene, TMEM53 , which encodes nuclear envelope transmembrane (NET) protein 53 (TMEM53, also known as NET4). (nature.com)
  • We discuss associated epigenetic and genome conformation changes elicited by the lamin A R482W mutation at the gene level. (frontiersin.org)
  • 14) strand proteins in gene-internal of FGFR3 and capable sunshine amyloid or proteotoxic cell increasing( Otsuki, 1999). (evakoch.com)
  • An abnormal congenital condition, associated with defects in the LAMIN TYPE A gene, which is characterized by premature aging in children, where all the changes of cell senescence occur. (lookformedical.com)
  • B) Luciferase reporter gene detection in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with expression in ZR75-1 and MDA-MB-231 cells, which were transfected with ZKSCAN5 shRNA, control shRNA, or ZKSCAN5 shRNA plus shRNA-resistant ZKSCAN5 (ZKSCAN5-R). The representative Western blot further showed the expression of ZKSCAN5. (bms-911543.com)
  • The 1,25D delicate HL60 cells had been compared with 1,25D resistant 40AF cells, and this recognized, for the first time, hematopoietic progenitor kinase one as the most upregulated MAPK gene within the one,25D resistant cells. (chk1inhibitor.com)
  • We initiated this review by investigating gene alterations in vitamin D resistant 40AF cells in contrast with their parental vitamin D delicate HL60 G cells, which might reversible PARP inhibitor explain the acquisition of resistance. (chk1inhibitor.com)
  • The different expression of isoforms H, M and L of synemin in the nervous system raises several questions about the regulation of synemin gene expression during the determination of glial and neuronal cell lineages in the central and the peripheral nervous system (CNS and PNS). (biomedcentral.com)
  • This selectivity suggests that the commitment of CNS precursor cells to form glia or neuron involves the direct regulation of the single synemin gene. (biomedcentral.com)
  • In the present study we examined the relationship between ceramide, ceramide metabolites and expression of the MDR1 gene in human breast cancer cell lines. (sphingolipidclub.com)
  • qPCR Primer Sets for a variety of gene targets and model organisms. (activemotif.com)
  • A) Schematic of the generation of OPTiKD human pluripotent stem cells (hPSCs) and of the resulting genetic circuit which allows tetracycline (TET)-dependent induction of gene knockdown both in hPSCs and hPSC-derived cells. (berterolab.com)
  • The vast majority of circumstances are attributable to a de novo single nucleotide mutation within the LMNA gene that prompts a cryptic splice donor web site, leading to manufacturing of a poisonous type of lamin A with a 50 amino acid inner deletion, termed progerin. (eumorphia.org)
  • However, using ACE, this is rapid (5 days), and provides the opportunity to complement the mutant in parallel, through concomitant insertion of a order ketoconazole cream wild type copy of the gene either under the check of its native promoter, or the strong fdx promoter. (centralparkcarriagesofficial.org)
  • Bold Gene Symbols correspond to current ENSEMBL IDs (GRCh37.p3, release 62), Gene Symbols in regular type may correspond to previous builds. (centralparkcarriagesofficial.org)
  • Open in another screen Fig. 1 SK2-produced S1P regulates hTERT proteins plethora and balance(A) Endogenous hTERT proteins plethora in A549 cells transfected with SK1 or SK2 siRNA. (lavoixdesrroms.org)
  • The cellular phenomena associated with laminopathy invariably show disruption of nucleoskeleton of lamina due to deregulated expression, localization, function, and interaction of mutant lamin proteins. (bvsalud.org)
  • Here, we review nuclear proteostasis and its deregulation in the context of lamin proteins and laminopathies. (bvsalud.org)
  • It has been suggested that synemin could function as a linker between different cytoskeletal components based on the fact that it interacts with several proteins involved in the organization of the costameres, neuromuscular and myotendinous junctions within striated muscle cells. (biomedcentral.com)
  • Our results establish a previously unreported SBD entity (craniotubular dysplasia, Ikegawa type) and contribute to a better understanding of the regulation of BMP signaling and bone formation. (nature.com)
  • Outcomes SK2-produced S1P promotes hTERT balance To examine the feasible assignments of S1P in the legislation of hTERT, we determined whether down-regulation of SK1 or SK2 affected hTERT balance or abundance in individual lung cancers cells. (lavoixdesrroms.org)
  • In addition to its roles in the regulation of inflammatory diseases and the maintenance of local immune cells, CCL5 expressed by cancer cells plays diverse roles in shaping cancer microenvironments toward their own survival. (nature.com)
  • Here we will focus on reviewing work describing the formation and regulation of the ovarian stem cell niche. (biomedcentral.com)
  • We established at mRNA level the expression of 84 genes partici pating inside the MAPK signaling network and cell cycle regulation employing Human MAP kinase RT2 Profile PCR Array. (chk1inhibitor.com)
  • Our study shows a developmental stage specific regulation of synemin isoforms in ES cells and its neural derivatives. (biomedcentral.com)
  • 2011) Mevalonate Cascade Regulation of Airway Mesenchymal Cell Autophagy and Apoptosis: A Dual Role since p53. (centralparkcarriagesofficial.org)
  • D) Cytoplasmic and nuclear ZKSCAN5 protein levels in two types of breast cancer cell lines, ZR75-1 and MDA-MB-231. (bms-911543.com)
  • Tubulin was used as the cytoplasmic control, and lamin A/C was used as the nuclear protein-loading control. (bms-911543.com)
  • Murine Lewis lung carcinoma (LLC) cells shaped smaller sized tumors in mice missing SK2 than in wild-type mice, and knocking down SK2 in LLC cells before implantation into mice suppressed their development. (lavoixdesrroms.org)
  • Interestingly, cells within tumors often exhibit a hierarchy of malignant potential, giving rise to the notion that small populations of cancer stem cells may be responsible for propagating certain cancers [ 1 , 2 ]. (biomedcentral.com)
  • In syngeneic mouse subcutaneous tumors formed by a mixture of A11 and P29 cells, the mitochondria-related EVs released from A11 cells reached distantly positioned P29 cells and CAFs. (biomedcentral.com)
  • We also show that ARF promotes p53 mutant stability in tumors and suppresses p73 mediated p21 expression and senescence. (oncotarget.com)
  • Pharmacologically inhibiting SK2 reduced the development Delphinidin chloride of subcutaneous A549 lung cancers cell-derived xenografts in mice, and appearance of wild-type hTERT, however, not an S1P-binding mutant, restored tumor development. (lavoixdesrroms.org)
  • Therefore, it might be safe to say that host CCL5 plays important roles in controlling cancer development but might create either pro- or anticancer environments according to the given situation, such as the type of cells that produce CCL5 and the type of cancers. (nature.com)
  • Non-small cell lung cancer (NSCLC) is one of the most common malignant cancers. (biomedcentral.com)
  • According to the latest statistics, lung cancer, of which 85% are non-small cell lung cancers(NSCLC), is the most commonly diagnosed cancer and the leading cause of cancer death in both men and women, the incidence and mortality of lung cancer have increased due to environmental pollution and an increasing number of smokers in recent years [ 1 ].The understanding of genetic alterations that drive NSCLC is evolving. (biomedcentral.com)
  • Furthermore, the frequency of predicted pathogenic mtDNA mutations was significantly correlated with distant metastasis in patients with non-small cell lung carcinoma (NSCLC) and colon cancers [ 2 ]. (biomedcentral.com)
  • Nuclear DNA damage can contribute to aging either indirectly (by increasing apoptosis or cellular senescence) or directly (by increasing cell dysfunction). (wikipedia.org)
  • The impact of JPX on Twist1 expression, cell growth, invasion, apoptosis, and in vivo tumor growth were investigated in lung cancer cells by western blotting, rescue experiments, colony formation assay, flow cytometry, and xenograft animal experiment. (biomedcentral.com)
  • CircHERC1 was significantly upregulated in lung cancer tissues and cells, ectopic expression of circHERC1 strikingly facilitated the proliferation, invasion and metastasis, and inhibited the apoptosis of lung cancer cells in vitro and in vivo. (biomedcentral.com)
  • The accumulation of FOXO1 in the cytosol and nuclear exclusion promoted cell proliferation and inhibited apoptosis. (biomedcentral.com)
  • In addition, circHERC1 can promote cell proliferation and inhibit apoptosis by sequestering FOXO1 in the cytoplasm to regulate AKT activity and BIM transcription. (biomedcentral.com)
  • Natural product anticancer agents enhance intracellular levels of ceramide, a sphingolipid that promotes cell apoptosis. (sphingolipidclub.com)
  • In addition, the pathological process of RA is promoted through the synergistic action of the cellular resident in the bone and in joint compartments, such as osteoclasts, chondrocytes and stromal cells ( Komatsu and Takayanagi, 2022 ). (elifesciences.org)
  • Gain- and loss-of-function experiments were implemented to observe the impacts of circHERC1 on the growth, invasion, and metastasis of lung cancer cells in vitro and in vivo. (biomedcentral.com)
  • Our findings indicate that circHERC1 facilitates the invasion and metastasis of NSCLC cells by regulating the miR-142-3p/HMGB1 axis and activating the MAPK/ERK and NF-κB pathways. (biomedcentral.com)
  • Mitochondrial DNA (mtDNA) carrying certain pathogenic mutations or single nucleotide variants (SNVs) enhances the invasion and metastasis of tumor cells, and some of these mutations are homoplasmic in tumor cells and even in tumor tissues. (biomedcentral.com)
  • We and others previously showed that certain mitochondrial DNA (mtDNA) somatic mutations or single nucleotide variants (SNVs) enhanced the invasion and metastasis of mouse and human cell lines, e.g. (biomedcentral.com)
  • To investigate the upstream management of MAPKs on this sys tem, we profiled the signaling networks applying MAPK/cell cycle mRNA arrays. (chk1inhibitor.com)
  • Outcomes MAPK mRNA profiles vary concerning vitamin D sensitive HL60 and vitamin D resistant 40AF cells. (chk1inhibitor.com)
  • Using RT-PCR, Western blotting and immunostaining, we show that synemin M is present at both mRNA and protein levels in undifferentiated ES cells as early as pluripotency factor Oct-3/4 and IF keratin 8. (biomedcentral.com)
  • Four wild-type (drug-sensitive) human breast cancer cell lines (MCF-7, T47D,MDA-MB-231, MDA-MB-435) were used to evaluate the influence of acute and chronic exposure to ceramide and ceramide metabolites on MDR1 mRNA,P-gp, and resistance to chemotherapeutic agents. (sphingolipidclub.com)
  • 72 hours) to C8- ceramide (5 μg/ml culture medium), a cell-permeable analog of ceramide, enhanced MDR1 mRNA levels by 3- and 5-fold in T47D and inMDA-MB-435 cells, respectively, but did not affect MCF-7 cells.D-erythro-sphingosine exposure (5 μg/ml, 72 hr) increased MDR1 mRNA levels in MDA-MB-435 cells by 3.5-fold. (sphingolipidclub.com)
  • Acute exposure ofMCF-7 and MDA-MB-231 cells to C8-glucosylceramide (10 μg/ml culture medium), a cell-permeable analog of glucosylceramide, increased MDR1 mRNA levels by 2- and 4-fold, respectively. (sphingolipidclub.com)
  • Here, we discover a previously unknown type of SBD in four independent families caused by bi-allelic loss-of-function pathogenic variants in TMEM53 , which encodes a nuclear envelope transmembrane protein. (nature.com)
  • Lamins are fundamental structural the different parts of the nuclear lamina, an intermediate filament meshwork that is situated under the internal nuclear membrane, attaching chromatin domains towards the nuclear periphery and localizing some nuclear envelope Delphinidin chloride protein. (lavoixdesrroms.org)
  • A single-stranded DNA-binding protein that is found in EUKARYOTIC CELLS. (lookformedical.com)
  • A ubiquitously expressed telomere-binding protein that is present at TELOMERES throughout the cell cycle. (lookformedical.com)
  • MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
  • DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
  • Our data reveal that the amino acid substitutions and deletions between variants impact the abundance and type of glycans on glycosylation sites of the spike protein. (cdc.gov)
  • In comparison with our previously reported site-specific glycan analysis on the S-D614G and its ancestral protein, glycan types on later variants showed high similarity on the site-specific glycan content to S-D614G. (cdc.gov)
  • Detailed site-specific glycan analysis of a wide variety of SARS-CoV-2 variants provides useful information toward the understanding of the role of protein glycosylation on viral protein structure and function and development of effective vaccines and therapeutics. (cdc.gov)
  • To understand the DNA damage theory of aging it is important to distinguish between DNA damage and mutation, the two major types of errors that occur in DNA. (wikipedia.org)
  • In a population of cells, mutant cells will increase or decrease in frequency according to the effects of the mutation on the ability of the cell to survive and reproduce. (wikipedia.org)
  • Given these properties of DNA damage and mutation, it can be seen that DNA damages are a special problem in non-dividing or slowly dividing cells, where unrepaired damages will tend to accumulate over time. (wikipedia.org)
  • On the other hand, in rapidly dividing cells, unrepaired DNA damages that do not kill the cell by blocking replication will tend to cause replication errors and thus mutation. (wikipedia.org)
  • The hot-spot lamin A R482W mutation causing familial partial lipodystrophy of Dunnigan-type (FPLD2), affects lamin A association with chromatin at the nuclear periphery and in the nuclear interior, and is associated with 3-dimensional (3D) rearrangements of chromatin. (frontiersin.org)
  • In this study, we investigated whether mtDNA with the NADH dehydrogenase subunit 6 ( ND6 ) G13997A pathogenic mutation in highly metastatic cells can be horizontally transferred to low-metastatic cells and stromal cells in the tumor microenvironment. (biomedcentral.com)
  • When MitoTracker Deep Red-labeled high-metastatic Lewis lung carcinoma A11 cells carrying the ND6 G13997A mtDNA mutation were cocultured with CellLight mitochondria-GFP-labeled low-metastatic P29 cells harboring wild-type mtDNA, bidirectional transfer of red- and green-colored vesicles, probably mitochondria-related EVs, was observed in a time-dependent manner. (biomedcentral.com)
  • The existence of niches has long been predicted from mammalian studies, but identifying stem cells in their native environments in vivo has remained a challenge in most vertebrates. (biomedcentral.com)
  • Functionally, JPX promoted lung cancer cell proliferation in vitro and facilitated lung tumor growth in vivo. (biomedcentral.com)
  • Our in vivo analysis also confirmed the expression of synemins H/M in multipotent neural stem cells in the subventricular zone of the adult brain, a neurogenic germinal niche of the mice. (biomedcentral.com)
  • Initial studies showed that two GBM cell lines (U87-MG and U138) transduced with CK2α had enhanced proliferation and anchorage-independent growth. (stanford.edu)
  • Thus, our data claim that S1P binding to hTERT mimicks phosphorylation allosterically, marketing telomerase balance and telomere maintenance therefore, cell proliferation, and tumor development INTRODUCTION Individual telomerase can be an RNA-dependent DNA polymerase which has a catalytic element, hTERT (individual telomerase invert transcriptase), and an interior RNA template, TR (1, 2). (lavoixdesrroms.org)
  • ZKSCAN5-Regulated VEGFC Promotes the Proliferation, Migration, and Tube Formation of HLECs Cancer cell-secreted VEGFC markedly enhanced the proliferation and migration of lymphocyte endothelial cells. (bms-911543.com)
  • Because ZKSCAN5 improved the secretion of VEGFC by breast cancer cells, the effects of the conditioned medium on HLEC proliferation and migration were investigated in ZKSCAN5 knockdown stable cell lines. (bms-911543.com)
  • The ZKSCAN5 knockdown ZR75-1 or MDA-MB-231 cell-conditioned medium decreased HLEC proliferation. (bms-911543.com)
  • The conditioned medium from these cells re-expressing ZKSCAN5 could rescue these effects ( Figures?2A, B ). A similar tendency was also detected in HLEC migration analysis ( Figures?2C, D ). Open in a separate window Figure?2 VEGFC secreted by cancer cells, under the influence of ZKSCAN5, regulates HLEC proliferation, migration, and tube formation. (bms-911543.com)
  • A, B) Cell proliferation and colony formation assays in HLECs cultured in conditioned medium come from ZR75-1 or MDA-MB-231 cells stably infected with lentivirus carrying ZKSCAN5 shRNA or ZKSCAN5 shRNA plus ZKSCAN5-R. The representative Western blot displays the expression of ZKSCAN5. (bms-911543.com)
  • A-type lamins also associate with chromatin in the nuclear interior, away from the peripheral nuclear lamina. (frontiersin.org)
  • Association of A-type lamins with chromatin at the nuclear periphery and in the nuclear interior. (frontiersin.org)
  • The FFPE ChIP assay comes in two modules, the Chromatin Preparation Kit (for which there are two options, depending on sample quality) and the ChIP-IT ® FFPE II Kit (to perform the ChIP-based enrichment), to allow flexibility dependent on type and amount of starting material. (activemotif.com)
  • In estimates made for mice, DNA lesions occur on average 25 to 115 times per minute in each cell, or about 36,000 to 160,000 per cell per day. (wikipedia.org)
  • In addition, intracranial injections of U87-MG cells with reduced GGT7 expression increased tumor growth in mice approximately 2-fold, and decreased mouse survival. (stanford.edu)
  • For tumour estimation, nude mice were inoculated subcutaneously with 1 107 ZR75-1 cells with different constructs on the right side. (bms-911543.com)
  • BALB/c mice were injected with 1 106 MDA-MB-231 cells labelled with luciferase carrying the indicated constructs into the lateral tail vein. (bms-911543.com)
  • We sought to identify PKA phosphorylation sites in PLIN5 and assess their functional relevance in cultured cells and the livers of mice. (uci.edu)
  • Re-expression of PLIN5 S155A in the liver of Plin5 liver-specific null mice reduced lipolysis compared with wild-type PLIN5 re-expression, but was not associated with other changes in hepatic lipid metabolism. (uci.edu)
  • The evidence supporting the claims of the authors is solid, although inclusion of the analysis of cell type-specific GLI1-deficient mice would have strengthened the study. (elifesciences.org)
  • On the behavioral stage, solely restricted and gentle alterations have been reported, with a big a part of analyzed behaviors being unaffected in GAMT KO as in contrast with wild-type mice. (eumorphia.org)
  • The multiple imaging modes afforded by widefield, confocal, and multiphoton fluorescence microscopies permit noninvasive, temporally resolved imaging of fixed and living cells and tissues with a high level of biochemical specificity. (microscopyu.com)
  • Without the self-renewing capacity of stem cells, these tissues quickly cease to function properly, leading to various conditions including infertility, anemia and immunodeficiency. (biomedcentral.com)
  • First proposed by Schofield in 1978 [ 3 ], the niche hypothesis posits that specific locations or microenvironments within tissues prevent the maturation of resident stem cells. (biomedcentral.com)
  • RT-qPCR and western blotting were conducted to detect the expression levels of lncRNA JPX and Twist1 in lung cancer cell lines and tissues. (biomedcentral.com)
  • Interestingly, JPX and Twist1 were coordinately upregulated in lung cancer tissues and cells. (biomedcentral.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • (a) By the end of larval development, approximately 100 primordial germ cells (PGCs) (red) populate the gonad and associate with cap cell precursor (dark green) and escort cell precursor cells (orange). (biomedcentral.com)
  • It remains unclear whether metastasis-enhancing pathogenic mutant mtDNA in tumor cells is intercellularly transferred between tumor cells and stromal cells. (biomedcentral.com)
  • These results suggest that metastasis-enhancing pathogenic mtDNA derived from metastatic tumor cells is transferred to low-metastatic tumor cells and stromal cells via S-EVs in vitro and in the tumor microenvironment, inferring a novel mechanism of enhancement of metastatic potential during tumor progression. (biomedcentral.com)
  • The recombinant AAV (rAAV) genome composed of a foreign expression cassette and AAV inverted terminal repeat (ITR) sequences exists in eukaryotic cells in an episomal form that is responsible for persistent transgene expression (Schnepp et al. (justia.com)
  • In the nervous system, synemin was found to associate with ruffled membranes of reactive astrocytes and to also co-localize with α-actinin, suggesting a role in cell motility [ 15 ]. (biomedcentral.com)
  • CCL5 is a unique chemokine with distinct stage and cell-type specificities for regulating inflammation, but how these specificities are achieved and how CCL5 modulates immune responses is not well understood. (nature.com)
  • Competing occupation for the sinks by different sources leads to distinct states of genomic equilibrium in differentiated cells. (biologists.com)
  • These effectors regulate the cytoskeleton, leading to a variety of cellular outputs including pronuclear migration, nuclear migration through constricted spaces, nuclear anchorage, centrosome attachment to nuclei, meiotic chromosome movements, and DNA damage repair. (ucdavis.edu)
  • Stem cells are essential for tissue homeostasis, particularly in organs that exhibit high rates of cellular turnover such as the skin, intestine and hematopoietic system. (biomedcentral.com)
  • The concept of the cellular niche represents one of the central paradigms in stem cell biology. (biomedcentral.com)
  • Twenty years following Schofield's seminal publication, Xie and Spradling provided compelling experimental evidence that a cellular niche supports the maintenance of germline stem cells (GSCs) in the Drosophila adult ovary [ 4 ]. (biomedcentral.com)
  • On the other hand, intercellular transfer of mitochondria and cellular components via extracellular vesicles (EVs) and tunneling nanotubes (TNTs) has recently attracted intense attention in terms of cell-to-cell communication in the tumor microenvironment. (biomedcentral.com)
  • Cellular studies of NTD and CTD oligomerization interface mutants demonstrate the importance of each interface in matrix assembly. (uci.edu)
  • Deletion of the proximal enhancer decreases CCL5 expression and augments the cytotoxic activity of tissue-resident T and NK cells, which coincides with reduced melanoma metastasis in mouse models. (nature.com)
  • These findings suggest that lncRNA JPX, a mediator of Twist1 signaling, could predispose lung cancer cells to metastasis and may serve as a potential target for targeted therapy. (biomedcentral.com)
  • Here, we focus on the Drosophila ovarian germline stem cell niche and review recent studies that have begun to reveal how intricate crosstalk between various signaling pathways regulates stem cell maintenance, how the extracellular matrix modulates the signaling output of the niche and how epigenetic programming influences cell development and function both inside and outside the niche to ensure proper tissue homeostasis. (biomedcentral.com)
  • Further mechanistic research indicated that circHERC1 acted as a competing endogenous RNA of miR-142-3p to relieve the repressive effect of miR-142-3p on its target HMGB1, activating the MAPK/ERK and NF-κB pathways and promoting cell migration and invasion. (biomedcentral.com)
  • However, multidrug-resistant (MDR) cancer cells may avoid the cytotoxic effects of chemotherapy by glycosylating ceramide. (sphingolipidclub.com)
  • Telomerase expands the ends of chromosomes and protects telomeres from replication-dependent attrition, allowing cancer tumor cells to proliferate indefinitely by conquering the finish replication issue (3C5). (lavoixdesrroms.org)
  • We address recent data showing a rewiring of such interactions in cells from FPLD2 patients, and in adipose progenitor and induced pluripotent stem cell models of FPLD2. (frontiersin.org)
  • These findings represent the first evidence that synemins could potentially be useful markers for distinguishing multipotent ES cells from undifferentiated neural stem cells and more committed progenitor cells. (biomedcentral.com)
  • p 0.01 versus the control shRNA group (A, B). (C, D) Wound healing assays for HLECs cultured in conditioned medium from ZR75-1 or MDA-MB-231 cells, which were Glutaminase-IN-1 stably infected as in (A). The image shown is one of the representative results (C, D). Scale bar: 100 m. (bms-911543.com)
  • Knocking down synemin in ES cells by shRNA lentiviral particles transduction has no influence on expression of Oct4, Nanog and SOX2, but decreased keratin 8 expression. (biomedcentral.com)
  • The OPTiKD method has been successfully used with a variety of shRNA designs (Bertero et al. (berterolab.com)
  • Organisms usually contain large numbers of sphingolipid subspecies (for a pathway based compilation, see www.sphingomap.org) and knowledge about the types and amounts is imperative because they influence membrane structure, interactions with the extracellular matrix and neighboring cells, vesicular traffic and the formation of specialized structures such as phagosomes and autophagosomes, as well as participate in intracellular and extracellular signaling. (sphingolipidclub.com)
  • During embryonic development, stem cells undergo the differentiation process so that they can specialize for different functions within the organism. (bvsalud.org)
  • 24 Distinctive isoforms of p38 MAPK, together with gamma and delta, which are not inhib ited by SB, also contribute towards the differentiation of HL60 and U937 cells. (chk1inhibitor.com)
  • 26 29 Yet, in spite of a lot of reviews for the function of important MAPK cascade kinases and their target genes in hematopoiesis,thirty it is not clear what reg ulates the MAPK cascades in VDD induced cell differentiation. (chk1inhibitor.com)
  • Subsequent scientific studies in quite a few selleckchem VER 155008 AML cell lines uncovered that HPK1 signaling can offer a dual function, each as a regulator of AML cell differentiation/cell cycle and like a mediator of resistance to vitamin D derivatives. (chk1inhibitor.com)
  • Morphological changes during cell differentiation are linked to IF network remodeling. (biomedcentral.com)
  • However, little is known concerning the presence and the role of IFs in embryonic stem (ES) cells and during their differentiation. (biomedcentral.com)
  • We have examined the expression profile of synemin isoforms in mouse pluripotent ES cells and during their neural differentiation induced by retinoic acid. (biomedcentral.com)
  • (A) Lamina-associated domains (LADs), at the nuclear envelope, schematized from analyses of cell populations using genome-wide approaches such as DamID- or ChIP-sequencing. (frontiersin.org)
  • Loss of CK2α decreased two β-catenin-regulated genes that are involved in GBM-initiating cell growth, OCT4 and NANOG. (stanford.edu)
  • A) Luciferase reporter genes were determined in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with Glutaminase-IN-1 different concentrations of reporter and myc-ZKSCAN5. (bms-911543.com)
  • The main ity of genes studied enhanced their expression in 40AF cells, and the 27 genes upregulated more than 2 fold, 10 with statistical significance, are listed in Table one. (chk1inhibitor.com)
  • Invited Perspective in The Journal of Cell Biology . (ucdavis.edu)
  • The complexity of the sphingolipidome is hard to imagine, but one hopes that, when partnered with other systems biology approaches, the causes and consequences of the complexity will explain how these intriguing compounds are involved in almost every aspect of cell behavior and the malfunctions of many diseases. (sphingolipidclub.com)
  • Casein kinase 2a regulates glioblastoma brain tumor-initiating cell growth through the ß-catenin pathway. (stanford.edu)
  • The resistance of 40AF cells to 1,25D may be overcome by enhancing one,25D action by simultane ous addition to 1,25D of carnosic acid, a plant derived antioxi dant, and SB202910, a selective inhibitor of isoforms and B of p38 MAPK,25 the mixture, referred to as DCS. (chk1inhibitor.com)
  • The niche model is consistent with many observations made in mammalian cell transplantation experiments, but difficulties in unequivocally identifying individual stem cells within their native environment prevented further testing of this hypothesis. (biomedcentral.com)
  • Intermediate filaments (IFs) are major components of the mammalian cytoskeleton and expressed in cell-type-specific patterns. (biomedcentral.com)
  • Other studies reported that some cancer cells could repress their own CCL5 expression to hinder migration of anticancer immune cells toward cancer sites 15 , 16 . (nature.com)
  • Here we identify two stage-specific enhancers: the proximal enhancer mediates the constitutive CCL5 expression during the steady state, while the distal enhancer located 1.35 Mb from the promoter induces CCL5 expression in activated cells. (nature.com)
  • Recent studies using neutralizing anti-CCL5 antibodies have suggested that the constitutive expression of CCL5 plays a regulatory role in maintaining tissue-resident memory (Trm) T cells in the human vaginal tract 9 and mouse skin 10 , suggesting that constitutive CCL5 expression in noninflamed conditions, hereafter referred to as homeostatic CCL5 expression, might be important for homeostasis of tissue-resident lymphocytes. (nature.com)
  • Expression of phosphorylation-defective PLIN5 S155A in Plin5 null cells resulted in decreased rates of lipolysis and triglyceride-derived fatty acid oxidation. (uci.edu)
  • Beyond this protective role, Mrr can inflict chromosomal DNA damage that elicits the SOS response in the host cell upon heterologous expression of specific methyltransferases such as M.HhaII, or after exposure to high pressure (HP). (uci.edu)
  • Exposure of cells to either octanoic acid (C8:0), a C8-ceramide hydrolysis product, or oleic acid (C18:1) did not affect MDR1 expression. (sphingolipidclub.com)
  • These data are the first evidence that chronic exposure to ceramide and its metabolites enhances expression of the MDR phenotype in cancer cells. (sphingolipidclub.com)
  • In this study, GLI1 expression in the synovial tissue of RA patients was positively correlated with RA-related scores and was highly expressed in collagen-induced arthritis (CIA) mouse articular macrophage-like cells. (elifesciences.org)
  • To determine the importance of CK2α in GBM stem cell maintenance, we reduced CK2α activity in primary GBM samples and tumor spheres derived from GBM patients. (stanford.edu)
  • Many of the mechanistic insights into how niches regulate stem cell maintenance have been obtained using invertebrate models such as Drosophila . (biomedcentral.com)
  • Therefore, host CCL5 can obviously work as an anticancer molecule to mount relevant immune responses against some cancer cells. (nature.com)
  • B) Immunoprecipitation for FLAG after that Traditional western blotting for hTERT in lysates from wild-type, SK1-lacking or SK2-lacking MEFs expressing vector or FLAG-hTERT in. (lavoixdesrroms.org)
  • No human disease has been associated with wild-type AAV infection and low toxicity is observed in human subjects following muscle transduction by rAAV (Manno et al. (justia.com)
  • Inhibition of CKα using siRNA or small-molecule inhibitors (TBBz, CX-4945) reduced cell growth, decreased tumor size, and increased survival rates in GBM xenograft mouse models. (stanford.edu)
  • We also verified that inhibition of CK2α decreased the activity of a well-known GBM-initiating cell regulator, β-catenin. (stanford.edu)
  • GSCs typically undergo asymmetric self-renewing divisions, producing one daughter stem cell that remains associated with the cap cell niche and a second daughter that is displaced away from the niche and as a result differentiates. (biomedcentral.com)
  • Persistent activation of immune cells leads to the progression of symptoms such as synovitis in RA. (elifesciences.org)
  • Prospectively identifying these cells and determining how they differ from their normal stem cell counterparts will probably provide important insights into the origin and progression of malignancy. (biomedcentral.com)