A knockout mouse , or knock-out mouse , is a genetically modified mouse ( Mus musculus ) in which researchers have inactivated, or "knocked out", an existing gene by replacing it or disrupting it with an artificial piece of DNA. (iiab.me)
A knockout mouse is a genetically modified mouse that has had one or more of its genes made inoperable through a gene knockout. (e-wit.pl)
genetically modified mouse used in gene research. (e-wit.pl)
Zebrafish2
The large-scale production and analysis of induced genetic mutations in worms, flies, zebrafish and mice have greatly accelerated the understanding of gene function in these organisms. (nature.com)
Because the genomes of the vertebrate model organisms mouse ( Mus musculus ) and zebrafish ( Danio rerio ) have also been sequenced, they offer the opportunity to test the function of every vertebrate gene, and by extension, the function of their human homologs. (nih.gov)
High-throughput1
It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain. (nature.com)
Targeted mutagenesis1
and (iii) the mouse genome supports targeted mutagenesis in specific genes by homologous recombination in embryonic stem (ES) cells, allowing genes to be altered efficiently and precisely. (nature.com)
Strains2
There are several thousand different strains of knockout mice. (iiab.me)
We typically produce CRISPR-mediated knockout mice in the C57BL/6 and FVB background, but we may be able to use other strains per your request. (e-wit.pl)
Rats2
Gene knockout in rats is much harder and has only been possible since 2003. (iiab.me)
Production of knockout rats using ENU mutagenesis and a yeast-based screening assay, Nat. (e-wit.pl)
Genes10
To date, published knockouts exist for only about 10% of mouse genes. (nature.com)
Current technology also permits insertion of 'reporter' genes into the knocked-out gene, which can then be used to determine the temporal and spatial expression pattern of the knocked-out gene in mouse tissues. (nature.com)
Despite these efforts, the total number of knockout mice described in the literature is relatively modest, corresponding to only ∼ 10% of the ∼ 25,000 mouse genes. (nature.com)
The curated Mouse Knockout & Mutation Database lists 2,669 unique genes (C. Rathbone, personal communication), the curated Mouse Genome Database lists 2,847 unique genes, and an analysis at Lexicon Genetics identified 2,492 unique genes (B.Z., unpublished data). (nature.com)
The converging interests of multiple members of the genomics community led to a meeting to discuss the advisability and feasibility of a dedicated project to produce knockout alleles for all mouse genes and place them into the public domain. (nature.com)
it can include regions preceding and following the coding DNA as well as introns between the … Wikipedia knockout mouse ( plural knockout mice ) ( biology , genetics ) Any of several forms of mice that have been genetically engineered by inactivating specific genes Wrong entry knockout mice [biol. (e-wit.pl)
A knockout mouse is a genetically engineered mouse in which one or more genes have been turned off through a targeted mutation. (e-wit.pl)
Conditional gene knockouts in mice are often used to study human diseases because many genes produce similar phenotypes in both species. (e-wit.pl)
More recently, Dr. Burgess has turned his mutagenesis efforts to targeted knockouts using CRISPR/ Cas9 technology, gaining full control over which genes are inactivated. (nih.gov)
Blastocyst2
Embryonic stem cells are isolated from a mouse blastocyst (a very young embryo ) and grown in vitro . (iiab.me)
The procedure used for producing the Blastocyst for production of knockout mice. (e-wit.pl)
Laboratory mouse1
A laboratory mouse in which a gene affecting hair growth has been knocked out (left), is shown next to a normal lab mouse. (iiab.me)
Genome4
Mouse knockout technology provides a powerful means of elucidating gene function in vivo , and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. (nature.com)
Now that the human and mouse genome sequences are known 1 , 2 , 3 , attention has turned to elucidating gene function and identifying gene products that might have therapeutic value. (nature.com)
Appreciation of the power of mouse genetics to inform the study of mammalian physiology and disease, coupled with the advent of the mouse genome sequence and the ease of producing mutated alleles, has catalyzed public and private sector initiatives to produce mouse mutants on a large scale, with the goal of eventually knocking out a substantial portion of the mouse genome 12 , 13 . (nature.com)
Knockout Mouse Project (KOMP) KOMP is a trans-NIH initiative to generate a public resource of mouse embryonic stem cells containing a null mutation in every gene in the mouse genome. (e-wit.pl)
Significantly1
Moreover, mice infused with GM-CSF knockout CAR-T cells have significantly lower serum levels of MCP-1, IL-6, MIG, and MIP-1 than mice … GCLC knockout mice die within a month of birth due to the absence of hepatic GSH synthesis. (e-wit.pl)
Genetic1
They are widely used in knockout experiments, especially those investigating genetic questions that relate to human physiology . (iiab.me)
Knocked-out gene1
White heterozygous mice can subsequently be crossed to produce mice that are homozygous for the knocked out gene. (iiab.me)
Technology1
Aspects of the technology for generating knockout mice, and the mice themselves have been patented in many countries by private companies. (iiab.me)
Sequence2
Usually, the new sequence is also given a marker gene, a gene that normal mice don't have and that confers resistance to a certain toxic agent (e.g., neomycin) or that produces an observable change (e.g. colour or fluorescence). (iiab.me)
In order to create knockout mice, a transgene with the desired sequence is inserted into an … (2003)", https://simple.wikipedia.org/w/index.php?title=Knockout_mouse&oldid=7302502, Creative Commons Attribution/Share-Alike License. (e-wit.pl)
Humans2
Mice are currently the laboratory animal species most closely related to humans for which the knockout technique can easily be applied. (iiab.me)
Consequently, observing the characteristics of knockout mice gives researchers information that can be used to better understand how a similar gene may cause or contribute to disease in humans. (iiab.me)
Cells3
Blastocysts containing cells, that are both wildtype and knockout cells, are injected into the uterus of a foster mother. (iiab.me)
For this example, we will take stem cells from a white mouse. (iiab.me)
however, IL-6 knockout mice receiving wild-type CAR-T cells were not protected from CRS. (e-wit.pl)
Model1
A knockout mouse (left) that is a model for obesity, compared with a normal mouse. (iiab.me)
Specific1
By causing a specific gene to be inactive in the mouse, and observing any differences from normal behaviour or physiology, researchers can infer its probable function. (iiab.me)
Article2
Mouse From Wikipedia, the free encyclopedia Jump to navigation Jump to search This article is about the animal. (e-wit.pl)
La ĉi-suba teksto estas aŭtomata traduko de la artikolo Knockout mouse article en la angla Vikipedio, farita per la sistemo GramTrans on 2018-01-09 03:21:03. (e-wit.pl)
Complete1
Many of the targeted alleles are designed so that they can generate both complete and conditional gene knockout mice. (e-wit.pl)