• Aberrant PAX3 and PAX7 expression. (wikipedia.org)
  • 2007 ). During this process, cells from the anterior area of the somite that specific the transcription factors Pax3 and Pax7 change into positioned exterior to the slow muscle within the exterior cell layer (ECL), which has traits of the amniote dermomyotome ( Hollway et al. (wijvallenaf.nl)
  • Finally we found that in an mouse model of fracture healing where muscle mass progenitor cells were lineage-traced Nkx3.2 and Sox9 are significantly upregulated while Pax3 is significantly downregulated in the muscle mass progenitor cells that give rise to chondrocytes during fracture restoration. (healthyguide.info)
  • Therefore our and analyses suggest that the balance of Pax3 Nkx3.2 and Sox9 may act as a molecular Talniflumate switch during the chondrogenic differentiation of muscle mass progenitor cells which may be important for fracture healing. (healthyguide.info)
  • We found that in the descendents of muscle mass progenitors that contributed to cartilage formation Nkx3.2 and Sox9 were strongly induced while Pax3 manifestation was strongly repressed. (healthyguide.info)
  • Facial and somatic muscle development is controlled by different transcription factors: PITX2 for facial muscles, and PAX3 and PAX7 for somatic muscles, suggesting the possibility that the FSHD disease gene, DUX4, is regulated and functions differently in facial and somatic muscles and will be responsive to different therapeutics. (fshfriends.org)
  • We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)
  • 30] The additional approach used a transgenic reporter mouse for Pax3 a paralog of Pax7 which allowed for the immediate isolation of Pax3+ (GFP+) muscle satellite television cells by movement cytometry. (biotech-angels.com)
  • Skeletal muscle regeneration is regulated by coordinated activation of multiple signaling pathways. (elifesciences.org)
  • However, the role of individual arms of the UPR in skeletal muscle regeneration remain less understood. (elifesciences.org)
  • Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
  • Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
  • Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
  • The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
  • However, the mechanisms of muscle regeneration are not yet fully understood. (elifesciences.org)
  • Therefore, medications that can prevent the progression of muscle atrophy or promote muscle regeneration are in high demand. (biomedcentral.com)
  • Considering that reinnervation of the TA muscle can occur even after UVFP through these anastomoses, it would be ideal if drugs that promote nerve regeneration could be used to treat UVFP. (biomedcentral.com)
  • Satellite cells (SCs), which are precursors of skeletal muscles, such as the TA muscle, play a key role in muscle homeostasis and regeneration. (biomedcentral.com)
  • SCs are normally in a quiescent state in adult muscles, and start proliferating during muscle regeneration in response to denervation. (biomedcentral.com)
  • Using genetic tools to conditionally abrogate C/EBPβ expression in Pax7 + cells, we examined the role of C/EBPβ in self-renewal of satellite cells during muscle regeneration. (biomedcentral.com)
  • After a single muscle injury, C/EBPβ-deficient satellite cells fail to self-renew resulting in a reduction of satellite cells available for future rounds of regeneration. (biomedcentral.com)
  • After a second round of injury, muscle regeneration is impaired in C/EBPβ conditional knockout mice compared to wild-type control mice. (biomedcentral.com)
  • These findings demonstrate that C/EBPβ is a novel regulator of satellite cell self-renewal during muscle regeneration acting at least in part through Notch2. (biomedcentral.com)
  • Further, C/EBPβ-deficient SCs are unable to self-renew after muscle injury leading to a reduction in the SC pool such that it cannot efficiently support muscle regeneration after a second round of injury. (biomedcentral.com)
  • Muscle satellite cells make up a stem cell populace that is capable of differentiating into myocytes and contributing to muscle mass regeneration upon injury. (healthyguide.info)
  • Dumont's research program is divided into 4 axes: 1) characterizing the intrinsic mechanisms regulating the cell fate decision of muscle stem cells during myogenesis, 2) characterizing the interactions between muscle stem cells and their microenvironment (e.g., macrophages, fibro-adipogenic progenitors, vascular cells, fibrosis, hyperoxia) in muscle regeneration and diseases, 3) characterizing the impact of genetic myopathies (e.g. (chusj.org)
  • Loss of either Dll4 or Mib from the myofiber reduces SC numbers and impairs muscle regeneration. (sciety.org)
  • Satellite cells and skeletal muscle regeneration . (inrs.ca)
  • In recent research, scientists unlocked ways to boost muscle regeneration in the experimental animal by activating the precursors of muscle cells. (sciencetrend.ca)
  • While many believe muscle regeneration is controlled by the body, it can be enhanced through proper nutrition and supplementation. (sciencetrend.ca)
  • In recent research, scientists managed to activate the precursors of muscle cells, which contributes to aiding muscular regeneration and rebuilding damaged tissue. (sciencetrend.ca)
  • Salk Institute researchers are finding methods to speed up methods to accelerate the regeneration of muscle tissue, utilizing a combination of molecular compounds that are generally utilized in the stem-cell analysis. (sciencetrend.ca)
  • 2021), the investigators confirmed that utilizing these compounds increased the regeneration of muscle cells in mice by activating the precursors of muscle cells, known as myogenic progenitors. (sciencetrend.ca)
  • The analysis offers perception into the underlying mechanisms associated with muscle regeneration and growth and will help athletes and aging adults regenerate tissue more effectively. (sciencetrend.ca)
  • 2004). However, in this research, Salk Institute researchers uncover particular elements that are in a position to speed up muscle regeneration and reveal the mechanism by which this occurred. (sciencetrend.ca)
  • Within the myofiber-specific model, they discovered that adding the Yamanaka factors accelerated muscle regeneration in mice by decreasing the levels of a protein referred to as Wnt4 in the area of interest, which activated the satellite cells. (sciencetrend.ca)
  • In contrast, Yamanaka factors didn't activate satellite cells within the satellite-cell-specific model and didn't enhance muscle regeneration, suggesting that Wnt4 performs an essential role in muscle regeneration. (sciencetrend.ca)
  • An absolute requirement for Pax7-positive satellite cells in acute injury-induced skeletal muscle regeneration. (sciencetrend.ca)
  • In vivo partial reprogramming of myofibers promotes muscle regeneration by remodeling the stem cell niche. (sciencetrend.ca)
  • 1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
  • 6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
  • Defective muscle regeneration can severely affect re-innervation by motor axons, and neuromuscular junctions (NMJs) development, ultimately leading to skeletal muscle atrophy. (biomed.news)
  • In particular, in skeletal muscle, specific semaphorin molecules are involved in the restoration and remodeling of the nerve-muscle connections, thus emphasizing their plausible role to ensure the success of muscle regeneration. (biomed.news)
  • This review article aims to discuss the impact of aging on skeletal muscle regeneration and NMJs remodeling and will highlight the most recent insights about the role of semaphorins in this context. (biomed.news)
  • Conditions involving muscles wasting such as for example muscular dystrophies cachexia and sarcopenia would reap the benefits of strategies URMC-099 that promote skeletal muscles regeneration. (biotech-angels.com)
  • Their fate is regulated by the expression of various transcription factors, including paired box 7 (Pax7) and myogenic differentiation (MyoD) [ 6 ]. (biomedcentral.com)
  • Develop transcription factor over-expression in combination with improved directed differentiation strategies to generate MuSCs from hPSCs with genetic and functional signatures of the in vivo MuSCs. (ca.gov)
  • The balance between differentiation and self-renewal is critical to maintain muscle tissue homeostasis, and alterations in this equilibrium can lead to chronic muscle degeneration. (biomedcentral.com)
  • The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)
  • Persistent expression of C/EBPβ upregulates Pax7, inhibits MyoD, and blocks myogenic differentiation. (biomedcentral.com)
  • CCAAT/enhancer binding proteins (C/EBPs) are a family of transcription factors that regulate cellular growth and differentiation, metabolism, and inflammation [ 18 ]. (biomedcentral.com)
  • For example, these manuscripts establish that elevated Pax7 levels drive quiescence and low Pax7 levels correlate with differentiation. (sciety.org)
  • Constitutive expression of Yap maintains Pax7 + and MyoD + satellite cells and satellite cell-derived myoblasts, promotes proliferation but prevents differentiation. (kcl.ac.uk)
  • Recently, we have developed a human skeletal muscle organoid differentiation system, which recapitulates myogenesis (Mavrommatis et al. (ruhr-uni-bochum.de)
  • The Wnt signaling pathway is involved in the control of cell proliferation and differentiation during skeletal muscle development. (biomedcentral.com)
  • Objective: The aim of this study was to reveal the role and regulatory mechanism of miR-188-5p in the proliferation and differentiation of goat muscle satellite cells. (koreascience.kr)
  • Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. (koreascience.kr)
  • Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. (koreascience.kr)
  • Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. (koreascience.kr)
  • Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. (koreascience.kr)
  • SFK inhibition also induced muscle differentiation in RMS cells by affecting the NOTCH3 receptor-p38 mitogen-activated protein kinase (MAPK) axis, which regulates the balance between proliferation and differentiation. (oncotarget.com)
  • Paired box protein Pax-7 is a protein that in humans is encoded by the PAX7 gene. (wikipedia.org)
  • Muscle stem cell from young mouse stained with the transcription factor Pax7 and in contact with the muscle fibre through the extracellular matrix protein M-Cadherin. (pasteur.fr)
  • This matrix protein is decreased in muscle stem cells in old mice thereby destabilising contact with the fibre. (pasteur.fr)
  • Feeding with amino acids can stimulate protein synthesis in all tissues, especially in skeletal muscle as reviewed by Davis et al. (nature.com)
  • Consequently, the primary destiny of ingested amino acids is incorporation into muscle protein to replete the reserves of amino acids misplaced in the fasting state. (wijvallenaf.nl)
  • Protein from soy, particularly, has been shown to improve weight-loss success, upkeep of muscle mass, and most weight-loss success in the long run," says McDaniel. (wijvallenaf.nl)
  • The basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor family and is a key protein in regenerative medicine. (biomedcentral.com)
  • PKU19F_G18_19 - Muscle blind-like Protein 2. (gao-lab.org)
  • We demonstrate enrichment of RUNT family transcription-factor-binding motifs in human epidermal CD8+CD103+CD49a+ TRM cells, paralleled by high RUNX2 and RUNX3 protein expression. (scilifelab.se)
  • The data establishing a continuum of SCs and the relationship to Pax7 protein levels can largely be eliminated and referenced by the two former manuscripts. (sciety.org)
  • however, priority needs to be given to resistance exercise training, and secondarily to adequate protein intake should be part of this dietary regime to mitigate losing muscle mass. (biomed.news)
  • Upon activation satellite cells rapidly initiate MyoD expression which leads to the activation of myogenin and terminally differentiated structural muscle mass genes such as myosin heavy chain (MHC) [1] [3]. (healthyguide.info)
  • All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)
  • In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)
  • More proliferation was observed in muscle tissue of LBW-GLN than LBW-ALA piglets at 5 dpn, but there was no clear effect of supplementation on related gene expression. (nature.com)
  • The use of a muscle creatine kinase germline knockout of the gene encoding Ire1/Ern1 shifts the focus from traditional studies of tissue repair toward the regenerating myofibers. (elifesciences.org)
  • As a sequence-specific DNA-binding transcription factor, any direct functional role played by Pax7 during development is mediated through target gene selection. (edu.au)
  • To assess the capacity of Pax7 to regulate transcription from these loci, we have cloned alternate transcripts of Pax7 (differing significantly in their DNA binding domain) into expression vectors and transfected cultured cells with these constructs, then analyzed target gene expression levels using RT-PCR. (edu.au)
  • We will perform single cell RNA sequencing (scRNA-seq) of embryonic, fetal, juvenile and adult muscle stem cells (MuSCs) and identify unique gene signatures and cell states across human myogenesis. (ca.gov)
  • Gene-free induction protocols for facial and somatic myogenesis have been developed, providing a unique opportunity to investigate and compare the regulation and disease functions of DUX4 in these two different muscle groups, both of which are affected FSHD disease. (fshfriends.org)
  • The abundance of Dll4 captured by the QSC correlates with levels of the SC identity gene, Pax7. (sciety.org)
  • Various growth factors, cytokines, and prostaglandins upregulate HGF gene expression, including basic fibroblast growth factor, oncostatin M, hypoxia-inducible factor 1α and nuclear factor-κB (NF-κB) ( 9 ). (spandidos-publications.com)
  • By contrast, transforming growth factor (TGF)-β1 was demonstrated to markedly downregulate HGF gene expression ( 10 , 11 ). (spandidos-publications.com)
  • The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development. (biomedcentral.com)
  • Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development. (biomedcentral.com)
  • Pax7 is a transcription factor that plays a role in myogenesis through regulation of muscle precursor cells proliferation. (wikipedia.org)
  • Clearance of defective muscle stem cells by senolytics restores myogenesis in myotonic dystrophy type 1 (2022). (chusj.org)
  • Senolytics target defective muscle stem cells and restore myogenesis in myotonic dystrophy type 1. (chusj.org)
  • MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. (biomedcentral.com)
  • Sonic Hedgehog (Shh) signaling helps pattern the vertebrate neural tube, in part by regulating the dorsal/ventral expression of a number of homeodomain containing transcription factors. (zfin.org)
  • Myofiber-specific ablation of IRE1α dampens Notch signaling and canonical NF-κB pathway in skeletal muscle of adult mice. (elifesciences.org)
  • As increased neuromuscular junctions are expected to maintain myofiber volume, bFGF administration may prevent thyroarytenoid muscle atrophy in the mid to long term. (biomedcentral.com)
  • The identification of heterogenous distribution of factors across the myofiber is an important contribution for dissecting how muscle stem cell diversity in a tissue is achieved. (sciety.org)
  • Understanding the effects of strabismus surgery on muscle cell biological reactions and myofiber remodeling may suggest new approaches for improving surgical outcomes. (arvojournals.org)
  • There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)
  • 1 Adult Stem Cells 1.1 Satellite Cells Studies in the last decade have clearly proven that the regenerative ability of adult skeletal muscle is due to the satellite cell a quiescent stem cell population of muscle precursors located between the basal lamina and sarcolemma of each myofiber. (biotech-angels.com)
  • 30 31 One group took the approach of transplanting sole muscle fibers which demonstrated that every myofiber including 7 or fewer satellite television cells could create over 100 new myofibers in engrafted muscles. (biotech-angels.com)
  • Pax-7 plays a role in neural crest development and gastrulation, and it is an important factor in the expression of neural crest markers such as Slug, Sox9, Sox10 and HNK-1. (wikipedia.org)
  • Expression of muscle growth related genes was quantified with qPCR. (nature.com)
  • One significant issue with the manuscript is the "discovery" of an SC continuum related to the relative levels of Pax7 expression. (sciety.org)
  • In the original manuscript we acknowledged that SCs exist on a continuum-a gradual transition from one state to another, based on scRNA-seq studies and the present data (Dll4, Pax7 and Ddx6 expression). (sciety.org)
  • The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)
  • The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)
  • First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). (koreascience.kr)
  • The control of MuSC state depends on the expression levels of myogenic transcription factors. (biomed.news)
  • Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)
  • 31] Cells isolated from mature skeletal muscles displayed homogenous expression of Pax7 and contributed to both dietary fiber repair also to the muscle satellite television cell URMC-099 compartment subsequent their transplantation into dystrophic mice. (biotech-angels.com)
  • The delayed growth of LBW piglets is mainly due to a slower development of skeletal muscle as the myogenic activity is usually impaired in these animals during the fetal and early postnatal period 3 , 4 . (nature.com)
  • Evaluate myogenic ability and functional differences of embryonic, fetal, juvenile and adult MuSCs in fusion, proliferation, engraftment and repopulation assays in mouse models of muscle disease. (ca.gov)
  • Thus, we have sought to identify genes targeted by Pax7 during embryonic development using an unbiased chromatin immunoprecipitation (ChIP) cloning assay to isolate cis-regulatory regions bound by Pax7 in vivo. (edu.au)
  • This analysis gives us greater insight into the direct functional role played by Pax7 during embryonic development. (edu.au)
  • Somatic cells can be reprogrammed to a pluripotent embryonic stem cell-like state (induced pluripotent stem (iPS) cells) by combinations of the transcription factors OCT4, SOX2, NANOG, LIN28, KLF4 and cMYC (Takahashi et al. (ruhr-uni-bochum.de)
  • One such alternative cell fate is the adipogenic fate as Pax7(+) satellite cells isolated from solitary myofibers used adipogenic fate in addition ARHGAP1 to muscle mass fate significance of these factors we used a mouse fracture healing model inside a genetically altered reporter mouse where muscle mass progenitors were lineage-traced. (healthyguide.info)
  • 31] Down the road Sacco and colleagues proven that intra-muscular transplantation of an individual luciferase-expressing muscle stem cell isolated from Myf5 reporter mice led to intensive proliferation and contribution to muscle materials. (biotech-angels.com)
  • Furthermore these authors demonstrated that Pax7(+)luciferase(+) mononuclear cells could possibly be readily re-isolated offering proof for the self-renewal of the cell human population. (biotech-angels.com)
  • We previously reported that local injection of single, high-dose bFGF into the paralyzed vocal fold 1 month after RLN transection compensated for an atrophied TA muscle by inducing activation of SC proliferation and increasing the number of myoblasts in the RLN paralysis model of rats [ 12 ]. (biomedcentral.com)
  • Mitotically quiescent in mature muscle, they can be activated to proliferate and generate myoblasts to supply further myonuclei to hypertrophying or regenerating muscle fibres, or self-renew to maintain the resident stem cell pool. (kcl.ac.uk)
  • Finally, we confirm in myoblasts that Yap binds to Tead transcription factors and co-activates MCAT elements which are enriched in the proximal promoters of Yap-responsive genes. (kcl.ac.uk)
  • 3 20 21 Major factors underlining this poor outcome included low ability of myoblasts to migrate beyond the injection site[22 23 and poor survival of injected cells. (biotech-angels.com)
  • 34] Upon damage satellite television cells become activated providing rise to proliferating myoblasts which in turn fuse to existing muscle tissue fibers or even to additional myoblasts to URMC-099 create new myofibers to correct muscle tissue harm. (biotech-angels.com)
  • However, PAX7 is not required for spermatogenesis, as mice without PAX7+ spermatogonia show no deficits in fertility. (wikipedia.org)
  • Additionally, mice lacking PAX7 had delayed recovery of spermatogenesis following exposure to busulfan when compared to control mice. (wikipedia.org)
  • By examining the transcriptome and epigenome of individual muscle stem cells simultaneously, we found that DNA transcribes* more chaotically in older muscle stem cells compared to those in younger mice. (pasteur.fr)
  • In the present study, we demonstrate that IRE1α (also known as ERN1) and its downstream target, XBP1, are activated in skeletal muscle of mice upon injury. (elifesciences.org)
  • Glutamine is regarded as the most abundant amino acid in the body, is mainly synthesized in skeletal muscle 10 , and muscle tissue is the most important site for Gln storage 11 . (nature.com)
  • Finally, we show that template DNA cosegregates with Numb in label-retaining cells that express the self-renewal marker Pax7. (nih.gov)
  • Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population. (biomedcentral.com)
  • conclude that Dll4 and Mib present on myofibers maintain a continuum of SC fates providing SCs capable of regenerating muscle and repopulatin the SC niche. (sciety.org)
  • 2011). Satellite cells are located in a distinct segment between a layer of connective tissue (basal lamina) and muscle fibers (myofibers). (sciencetrend.ca)
  • The addition of BrdU-positive myonuclei increased from 1 per 1000 myofibers in cross sections of control muscles to 2 to 3 per 100 myofibers in the resected muscles. (arvojournals.org)
  • Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
  • Skeletal muscle is a complex tissue composed of thousands of individual multinucleated myofibers. (arvojournals.org)
  • It is considered postmitotic, in that the myonuclei residing within these myofibers do not divide in adult muscle. (arvojournals.org)
  • 3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)
  • Strikingly, some of these cells display selective template-DNA strand segregation during mitosis in the muscle fibre in vivo, as well as in culture independent of their niche, indicating that genomic DNA strands are nonequivalent. (nih.gov)
  • We show that Pax7 directly occupies sites within genes encoding transcription factors Gbx1 and Eya4, the neurogenic cytokine receptor ciliary neurotrophic factor receptor, the neuronal potassium channel Kcnk2, and the signal transduction kinase Camk1d in vivo and regulates the transcriptional state of these genes in cultured cells. (edu.au)
  • We will identify unique cell surface markers on in vivo derived MuSCs that distinguish PAX7-positive cells from other muscle cells and across cell states. (ca.gov)
  • Studies targeted at establishing such effects at cellular level and their correlations with in vivo models, will broaden our understanding of the roles played by androgens on skeletal muscle function in elderly. (frontiersin.org)
  • Transcription is the process by which genes carried by DNA are encoded in another form (RNA), this RNA is then translated into proteins. (pasteur.fr)
  • Yamanaka factors are a mix of proteins (referred to as transcription factors) that control how DNA is copied for translation into different proteins. (sciencetrend.ca)
  • PKU19F_G03_04 - Functional analysis of the transcription factor Pax7 in mouse muscle stem cells. (gao-lab.org)
  • PKU19F_G12_13 - Functional analysis of the transcription factor Pax7 in mouse muscle stem cells. (gao-lab.org)
  • 10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)
  • The muscle fiber is a multinucleated syncytium that serves as a niche to QSCs, raising the possibility that the muscle fiber regulates the diversity of states across the QSC pool. (sciety.org)
  • Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
  • Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
  • Skeletal muscle is a target tissue for anabolic steroids. (frontiersin.org)
  • The 'adipose tissue expandability model' identifies the limited capacity and dysfunctionality of WAT, preventing its expansion and accommodation of surplus of energy, as key determinants for the onset and progression of obesity-associated metabolopathologies as a result of ectopic deposition of toxic lipid species in metabolic organs (i.e. muscle or liver [also known as lipotoxic insult]) [ 7 ]. (springer.com)
  • For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)
  • Primary MuSCs isolated from individual skeletal muscles (gastrocnemius and masseter) are lysed, and their RNA is reverse-transcribed into cDNA and copied into double-stranded DNA. (biomed.news)
  • Satellite cells assure postnatal skeletal muscle growth and repair. (nih.gov)
  • PAX7 functions as a marker for a rare subset of spermatogonial stem cells, specifically a sub set of Asingle spermatogonia. (wikipedia.org)
  • Unlike PAX7+ muscle satellite cells, PAX7+ spermatogonia rapidly proliferate and are not quiescent. (wikipedia.org)
  • For this reason, the team of Shahragim Tajbakhsh from Institut Pasteur, in collaboration with the team of Wolf Reik at the Babraham (UK) tried to decipher the relationships between epigenetic regulation of DNA and ageing muscle stem cells. (pasteur.fr)
  • Muscle samples were collected and myogenic cells were isolated and cultivated. (nature.com)
  • Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)
  • 2007 ). The ECL is a supply of both primary dermal endothelial cells and proliferative MPCs that migrate via the somite to kind a second wave of lateral quick muscle fibres in the late embryo and larval levels ( Hollway et al. (wijvallenaf.nl)
  • 2007 ). The ECL may also provide a population of resident quiescent MPCs equivalent to mammalian satellite cells, which, as soon as activated, provide a supply of proliferative cells required for grownup muscle progress ( Hollway et al. (wijvallenaf.nl)
  • 2007 ). In some species the ECL disappears throughout the early larval levels, by which time a resident population of Pax7-expressing cells is present throughout the myotome ( Marschallinger et al. (wijvallenaf.nl)
  • Immunohistochemistry indicated that bFGF significantly increased the number of satellite cells in the thyroarytenoid muscle up to day 14 and that of neuromuscular junctions on days 28 and 56. (biomedcentral.com)
  • We determined the developmental and functional status of both skeletal muscle progenitor cells as well as adult human satellite cells. (ca.gov)
  • We are developing a target product profile for human satellite cells derived from human pluripotent stem cells which will be used to use muscle stem cells in personalized medicine for patients with devastating muscle wasting or degenerative diseases. (ca.gov)
  • We will molecularly and functionally define muscle stem cells in human muscle in development, juvenile and adult and develop strategies to generate the most regenerative muscle stem cells from hPSCs. (ca.gov)
  • A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. (ca.gov)
  • Intro Satellite cells are the cells specific stem cells in the adult skeletal muscle mass. (healthyguide.info)
  • These cells lay beneath the basement membrane of the muscle mass fiber and are usually mitotically quiescent [1]. (healthyguide.info)
  • Upon injury or when challenged with a variety of mechanical or biochemical stimuli satellite cells re-enter the cell cycle and give rise to Talniflumate differentiated myocytes which form new muscle mass materials or fuse with existing materials and contribute to muscle mass growth and restoration [1]. (healthyguide.info)
  • In the beginning satellite cells were considered to Talniflumate be unipotent stem cells with the ability of generating a unique specialized phenotype the skeletal muscle mass cells. (healthyguide.info)
  • Duchenne muscular dystrophy, Myotonic Dystrophy type 1) and acquired myopathies (e.g., prematurity) on muscle stem cell function, and 4) investigating new therapeutic avenues targeting defective muscle stem cells in various muscular dystrophies. (chusj.org)
  • Nicolas Dumont obtained his PhD at the Université Laval where he studied the regulatory network between inflammatory cells and skeletal muscles. (chusj.org)
  • Targeting muscle stem cells with bioactive lipids for the treatment of muscular dystrophies. (chusj.org)
  • Hepatocyte growth factor (HGF) is produced by stromal and mesenchymal cells, and it stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis in various organs via tyrosine phosphorylation of its cognate receptor, Met. (spandidos-publications.com)
  • HGF was cloned as a growth factor for hepatocytes ( 1 , 2 ), is identical to scatter factor (SF) and was originally discovered as a fibroblast-derived cell motility factor for epithelial cells ( 3 ). (spandidos-publications.com)
  • Satellite cells are the resident stem cells of skeletal muscle. (kcl.ac.uk)
  • We are currently focusing to model neural (Frontotemporal Dementia) and skeletal muscle (Duchenne, LGMD) diseases with human iPS cells and CRISPR/Cas9 genome editing. (ruhr-uni-bochum.de)
  • Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)
  • Another stimulus that activates satellite cells is stretching of the muscle. (arvojournals.org)
  • 7 8 9 In fact, passive stretching alone can activate limb muscle satellite cells. (arvojournals.org)
  • Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. (koreascience.kr)
  • In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. (koreascience.kr)
  • We demonstrate that transgenic AAVR overexpression greatly increases the efficiency of transduction of many diverse cell types, including muscle stem cells, which are normally refractory to AAV transduction. (nature.com)
  • This highly malignant tumor has traditionally been considered to arise from committed skeletal muscle precursor cells that fail to differentiate [ 1 ], although some RMSs develop in organs lacking a skeletal muscle component [ 2 , 3 ]. (oncotarget.com)
  • A single, early local administration of high-dose bFGF prevented atrophic changes in the thyroarytenoid muscles by activating satellite cell proliferation and reforming neuromuscular junctions. (biomedcentral.com)
  • Pax7 plays critical roles in development of brain, spinal cord, neural crest, and skeletal muscle. (edu.au)
  • Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)
  • This work could provide a cell therapy for muscle disorders including muscular dystrophies or sarcopenia. (ca.gov)
  • A large majority of California residents are affected or will be affected with a muscle disease in their lifetime including severe muscular dystrophies, aging related muscle loss called sarcopenia or muscle weakness. (ca.gov)
  • Sarcopenia, the age-related loss of skeletal muscle mass and function, is becoming more prevalent as the lifespan continues to increase in most populations. (frontiersin.org)
  • Testosterone concentrations decline as age increase, suggesting that low plasma testosterone levels can cause or accelerate muscle- and age-related diseases, as sarcopenia. (frontiersin.org)
  • Currently, there is increasing interest on the anabolic properties of testosterone for therapeutic use in muscle diseases including sarcopenia. (frontiersin.org)
  • This age-related decline in skeletal muscle mass and strength generation, the primary function of skeletal muscle mass, is known as sarcopenia ( 2 , 3 ). (frontiersin.org)
  • Indeed, most of the intrinsic as well as extrinsic (systemic) muscle changes that occur with age are believed to be involved in the development of sarcopenia ( 5 , 6 ). (frontiersin.org)
  • The term sarcopenia was first proposed in 1989 by Irwin Rosenberg to describe a multifactorial syndrome that occurs with age and results in loss of skeletal muscle mass and function ( 3 , 4 ). (frontiersin.org)
  • However, during aging, extensive injury, or pathological conditions, the complete regenerative program is severely affected, with a progressive loss of muscle mass and function, a condition known as sarcopenia. (biomed.news)
  • Research on regenerative medicine using basic fibroblast growth factor (bFGF) has recently advanced in the field of laryngology. (biomedcentral.com)
  • fibroblast growth factor re. (gsea-msigdb.org)
  • The cross-sectional thyroarytenoid muscle area was significantly larger in the high-dose group than in the saline and low-dose groups on days 28 and 56. (biomedcentral.com)
  • We previously reported that local administration of bFGF 1 month after recurrent laryngeal nerve (RLN) paralysis compensated for atrophy of the thyroarytenoid muscle. (biomedcentral.com)
  • These treatments provide only static adjustment to the larynx, and their effects may be limited by the remaining hoarseness due to progressive atrophy of the paralyzed thyroarytenoid (TA) muscle. (biomedcentral.com)
  • Below we review the literature on some of the most studied stem cell populations that have been ascribed with muscle regenerative potential pointing out their advantages as well as caveats. (biotech-angels.com)
  • The quiescent muscle stem cell (QSC) pool is heterogeneous and generally characterized by the presence and levels of intrinsic myogenic transcription factors. (sciety.org)
  • The objective of this study was to elucidate the effects of early bFGF administration on the thyroarytenoid muscle after RLN transection and to investigate the underlying mechanisms. (biomedcentral.com)
  • However, the pathophysiological mechanisms underlying this muscle syndrome and its relationship with plasma level of androgens are not completely understood. (frontiersin.org)
  • Our findings are expected to lay the groundwork for detailed cell and molecular investigations of facial muscle-specific mechanisms of FSHD disease. (fshfriends.org)
  • This study elucidated effects of glutamine (Gln) supplementation on the cellular muscle development of LBW and normal birth weight (NBW) piglets. (nature.com)
  • We observed a temporarily increased intramuscular availability of free Gln that could have influenced cellular processes in skeletal muscle. (nature.com)
  • Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)