Multipotent cell. Medical search. Frequent questions

This rubric exemplifies the utility of this cross-platform resource for deconvoluting the complexity of tran- scriptional programs controlling stem/progenitor cell fate in hematopoiesis. (lu.se)
These data support a luminal multilineage progenitor cell model for prostate tissue and establish a robust, scalable system for mechanistic studies. (bvsalud.org)
Areas of research focus are beta cell replacement therapies for the treatment of diabetes, the identification of lung progenitor cell populations involved in lung repair, and in the diagnosis of pancreatic cancer. (ohsu.edu)
In a study of one affected family, phacomatosis pigmentokeratotica was found to be caused by a postzygotic HRAS mutation in a multipotent progenitor cell. (medscape.com)

Remarkably, in a fashion reminiscent of quiescent stem cells, the slow replicative phenotype of undifferentiated FReP cells reverses after differentiation induction, with differentiating FReP cells proliferating faster and expressing less p15(Ink4B) and p21(WAF1/Cip1) than differentiating iPS cells. (ca.gov)
However, the direct differentiation of MCSs into endothelial cells is still matter of debate. (unipi.it)
Identifying the transcription factor interactions that are responsible for cell-specific gene expression programs is key to understanding the regulation of cell behaviors, such as self-renewal, proliferation, differentiation, and death. (ox.ac.uk)
The multilineage E-4031 dihydrochloride differentiation potential of clonally derived cells within a MSC population have been shown to be variable [5, 20-25], further demonstrating the heterogeneous nature of the defined MSC population. (buyresearchchemicalss.net)
Understanding key regulatory pathways and molecules either involved in maintaining MSC in their undifferentiated state or during the process of differentiation allows for a better handle on expanding and culturing these cells in large scale for therapeutic applications. (buyresearchchemicalss.net)
These microRNAs are expressed at high levels in several human embryonic stem cell (ESC) and human embryonal carcinoma cell (EC) lines [49, 50] and decrease upon differentiation into embryoid body (EB) for two weeks in culture [50] supporting their association with the stem cell state. (buyresearchchemicalss.net)
However, the underlying mechanisms related to paracrine effect or the differentiation of transplanted cells into neurogenic or angiogenetic cells are still not well understood. (biomedcentral.com)
Through fluorescence-activated cell sorting, microarray analysis, in vitro differentiation assays, mixed lymphocyte reaction, and a model of ischemic kidney injury, this study sought to identify and characterize multipotent organ stem/progenitor cells in the adult kidney. (tau.ac.il)
The generation of inducible stem cell lines with the Lent-ON-Plus LVs did not require selection or cloning, and transgene regulation was maintained after long-term cultured and upon differentiation toward different lineages. (nih.gov)
Adipose-derived MSCs, in particular, pose an attractive option for cell-based therapy due to their relatively decreased morbidity during isolation and potential for expansion and differentiation [ 12 ]. (hindawi.com)
Multipotential differentiation of MSCs into adipogenic, osteogenic, and chondrogenic cell lineages. (hindawi.com)
2016). Natural killer cell-based adoptive immunotherapy eradicates and drives differentiation of chemoresistant bladder cancer stem-like cells. (springer.com)
Stem cells are defined by their capacity for self-renewal and multilineage differentiation, making them uniquely situated to treat a broad spectrum of human diseases. (articlecity.com)
Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
Our research focuses on characterising the stem and progenitor cells that build the hypothalamus and characterising the molecular networks that direct hypothalamic morphogenesis, growth and differentiation. (sheffield.ac.uk)
The topics covered include nuclear reprogramming, regulation of stem cell self-renewal and differentiation, the stem cell niche, and signaling and gene regulation in stem cells. (cshlpress.com)
We isolated a new population of stem cells from human orofacial tissue gingiva, a tissue source easily accessible from the oral cavity, namely GMSC, which exhibited clonogenicity, self-renewal, and multipotent differentiation capacities. (ca.gov)
Human orofacial bone-derived mesenchymal stem cells (OMSCs) showed distinct differentiation traits from mesenchymal stem cells (MSCs) derived from long bones, mouse OMSCs have not been isolated due to technical difficulties, which in turn precludes using mouse models to study orofacial diseases. (ca.gov)
Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
We also found an unexpectedly dynamic pattern of transcript isoform regulation, suggesting a critical regulatory role during HSC differentiation, and a cell cycle/DNA repair signature associated with multipotency in MPP2 cells. (lu.se)
Objective: To establish cultures of cells from the pulp of permanent teeth by the explant method assessing parameters usually presented by stem cells, such as the expression of certain markers and the differentiation ability into osteogenic, adipogenic, and chondrogenic lineages. (bvsalud.org)
In addition, cells were assessed for multi-differentiation potential. (bvsalud.org)
Similarly, the cells showed differentiation ability into all three lineages of cells tested. (bvsalud.org)
The isolated cells may be considered stem cells, based on the current criteria for their characterization, such as plastic adherence, expression of certain markers, and the absence of others, as well as multi-differentiation potential, which showed to be promising for the application in tissue regeneration. (bvsalud.org)
that regulate the differentiation and activation of these cells. (cdc.gov)

The generation or isolation of safer and readily available stem cell sources will significantly aid clinical applications. (ca.gov)
Feline adult adipose tissue-derived multipotent stromal cell isolation" by C. Fargason, N. Zhang et al. (lsu.edu)
Isolation, expansion, and characterization of natural killer cells and their precursors as a tool to study cancer immunosurveillance. (springer.com)
This project focuses on the compelling need to generate cell-surface marker specific reagents useful for identification, study, and isolation of pulmonary stem/progenitor cells. (ohsu.edu)
Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
Isolation of multipotent adult stem cells from the dermis of mammalian skin. (bvsalud.org)
Conclusion: Our results suggest that the explant method - or cell proliferation method - is suitable for the isolation and culture of stem cells from dental pulp of permanent teeth. (bvsalud.org)
fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

Mesenchymal stromal cells (MSCs) represent a bone marrow (BM) population, classically defined by five functional properties: extensive proliferation, ability to differentiate into osteoblasts, chondrocytes, adipocytes, and stromal cells-supporting hematopoiesis. (unife.it)
Our findings reveal ErbB activation as a strong inductive signal for stem-cell proliferation. (nature.com)
Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
NK cell proliferation, phenotypic expression and cytotoxic activity were evaluated. (springer.com)
Following early observations that MSCs inhibit T-cell proliferation, 9 MSCs were found to interact with the majority of innate and adaptive immune cells. (bmj.com)
Materials and method: The pulp tissue, obtained from wisdom teeth, was placed in a 6-well plate containing proper culture medium, and stored at 37 °C and 5% CO2 for cell proliferation and plastic adherence. (bvsalud.org)
We have already acquired proof-of-principle showing that reprogramming towards astrocytic fate causes morphological changes, reduces proliferation and bestow astrocytic function in human GBM cell lines. (lu.se)
Hypoxia inducible factor-2α importance for migration, proliferation, and self-renewal of trunk neural crest cells. (lu.se)

Like virally-induced pluripotent stem (iPS) cells, FMOD reprogrammed (FReP) cells express pluripotency markers, form embryoid bodies (EBs), and differentiate into ectoderm, mesoderm, and endoderm derivatives in vitro. (ca.gov)
and the ability to differentiate in osteoblasts, chondrocytes, adipocytes, and stromal cells supporting hematopoiesis. (unife.it)
Indeed, MPCs are Nestin-positive CD31-positive cells exhibiting angiogenic potential that differentiate in MSC upon proper stimuli. (unipi.it)
We used the paradigmatic GATA-PU.1 axis to explore, at the systems level, dynamic relationships between transcription factor (TF) binding and global gene expression programs as multipotent cells differentiate. (lu.se)
Objective Human multipotent mesenchymal stromal cells (MSC) have the potential to differentiate into multiple cell types, although little is known about factors that control their fate. (buyresearchchemicalss.net)
Nevertheless, the possibility of obtaining MSC from an autologous NFATC1 source and their ability to differentiate into a variety of connective tissue types makes them ideal candidates for cell therapy. (buyresearchchemicalss.net)
MVSCs expressed markers such as Sox 17 and Sox10 and could differentiate into neural cells in vitro. (biomedcentral.com)
In vivo investigation has shown that MVSCs can be activated by vascular injuries, become proliferative, and differentiate into smooth muscle cells (SMCs). (biomedcentral.com)
In multicellular organisms, stem cells are undifferentiated or partially differentiated cells that can differentiate into various types of cells and proliferate indefinitely to produce more of the same stem cell. (wikipedia.org)
In vivo, they eventually differentiate into all of the body's cell types (making them pluripotent). (wikipedia.org)
They exist to replenish rapidly lost cell types and are multipotent or unipotent, meaning they only differentiate into a few cell types or one type of cell. (wikipedia.org)
they are vastly outnumbered by the progenitor cells and terminally differentiated cells that they differentiate into. (wikipedia.org)
Since 1998 however, it has been possible to culture and differentiate human embryonic stem cells (in stem-cell lines). (wikipedia.org)
Osteoprogenitor MSCs not only differentiate into bone, but they also exert modulatory effects on immune cells via a variety of mechanisms. (hindawi.com)
Multipotent mesenchymal stromal cells (MSCs), also known as mesenchymal stem cells, have the capacity to differentiate into a variety of cell types (Figure 1 ), including adipocytes, chondrocytes, and osteocytes [ 9 , 10 ]. (hindawi.com)
They are able to differentiate into any cell of an organism and have the ability of self-renewal. (articlecity.com)
Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)
By culturing these multipotent cells with proteins and minerals, Dr. Xu has already gotten the hair follicle stem cells to differentiate into skin, nerve, muscle, bone, cartilage, and fat tissue. (loe.org)
Neural stem cells can also give rise to neural progenitor cells, which proliferate rapidly during their short lives and then 'differentiate' into neurons or glia. (elifesciences.org)
Since neurosphere-forming cells can self-renew and differentiate into neurons and glia, the ability of cells to form neurospheres has generally been taken as evidence that they are stem cells. (elifesciences.org)

and [3] therapeutic innovation - FMOD reprogrammed (FReP) multipotent cells can be easily differentiated into skeletal myocytes and osteoblasts in vitro and in vivo, respectively, and will have significant clinical value in regenerative medicine to establish skeletal muscle and bone by autologous cells. (ca.gov)
These cells could be differentiated toward mesodermal lineage in vitro up to passage 20. (uzh.ch)
HBCs thus possess a conserved adhesion receptor expression profile similar to non-neural stem cells, preferential self-replication in an in vitro environment mimicking their in vivo niche, and contain subpopulations of cells that can produce multiple differentiated neuronal and glial progeny from within and beyond the olfactory system in vitro. (syr.edu)
These MVSCs can be differentiated into neural cells and mesenchymal cells in vitro. (biomedcentral.com)
However, when they are isolated and cultured in vitro, they can be kept in the stem-cell stage and are known as embryonic stem cells (ESCs). (wikipedia.org)
In vitro, they are plastic adherent and slowly proliferating and result in inhibition of alloreactive CD8 + T cells, indicative of an immuneprivileged behavior. (tau.ac.il)
Cell adhesion assays were carried out with a panel of endothelial cells from arteries, veins and the microvasculature in vitro . (mst.edu)
In this paper, we review the current literature on both in vitro and in vivo studies on the role of the immune system in fracture repair, the use of MSCs in the enhancement of fracture healing, and interactions between MSCs and immune cells. (hindawi.com)
The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expansion of NK cells. (springer.com)
In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
As a third major line of research, we explore, in collaboration with associate professor Henrik Ahlénius group if GBM cells can be reprogrammed to neuron like cells in vitro and in vivo. (lu.se)
In vitro maintenance of multipotent neural crest stem cells as crestospheres. (lu.se)
This paper gives an Islamic perspective on some of these advances, including abortion, in vitro fertilization, genetic engineering, cloning and stem cell research. (who.int)

OBJECTIVE: This study was undertaken to isolate and characterize multipotent mesenchymal stem cells from term human placenta (placenta-derived mesenchymal stem cells, PD-MSCs). (uzh.ch)
Furthermore, the CD146(+) MSCs secrete a complex combination of growth factors (GFs) controlling hematopoietic stem cells (HSCs) function, while providing a >2-log increase in the long-term culture (LTC) colony output in 8-week LTC over conventional assays. (unife.it)
Much of the work conducted on adult stem cells has focused on mesenchymal stem cells (MSCs) found within the bone marrow stroma. (livingroom.health)
PLA cells expressed multiple CD marker antigens similar to those observed on MSCs. (livingroom.health)
Finally, PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression. (livingroom.health)
Are MSCs angiogenic cells? (unipi.it)
Recent investigations have made considerable progress in the understanding of tissue regeneration driven by mesenchymal stromal cells (MSCs). (unipi.it)
According to this scenario, we hypothesize that the presence of the recently described Mesodermal Progenitor Cells (MPCs) within the MSCs cultures is responsible for their variable angiogenic potential. (unipi.it)
Cell-based therapy utilizing mesenchymal stromal cells (MSCs) is an attractive option for augmenting the fracture repair process. (hindawi.com)
The normal process of fracture repair begins with an immediate inflammatory response as the innate immune system (macrophages, monocytes, neutrophils, and NK cells) responds with a variety of cytokines that recruit and activate several cell types, including osteoprogenitor mesenchymal stem cells (MSCs), to the site of injury [ 1 , 2 ]. (hindawi.com)
Coupled with reports that allogeneic MSCs have immunoprivileged status and immunomodulatory properties, there has been considerable interest in exploring the use of these cells as a therapeutic option for bone repair. (hindawi.com)
MSCs are able to evade the host cell immune system due to their low expression of major histocompatibility complex (MHC) class I molecules and complete lack MHC class II molecules and other costimulatory molecules (CD40, CD40L, CD80, and CD86) required for immune cell stimulation [ 15 - 17 ]. (hindawi.com)
MSCs were obtained from the bone marrow (BM) cells remaining in the bag and filter used in the transplantation of hematopoietic stem cells. (springer.com)
NK expansion and activation were stimulated by culture with artificial antigen-presenting cells (aAPCs) and IL-2, in the presence or absence of BM-MSCs. (springer.com)
Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
1 Unique MSC-specific markers have not yet been identified, and MSCs constitute a heterogeneous cell population, including both multipotent (stem) cells and progenitor cells and might even contain pluripotent cell fractions. (bmj.com)
2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)
Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
Overall, the results presented in this study show a common mechanism of endocytosis for MSC EVs across different cell types and the feasibility to generate functionally enhanced EVs by modifications to parental MSCs. (frontiersin.org)

MSC = multipotent mesenchymal stromal cell. (hindawi.com)
Figure 1 Illustration demonstrating a hypothetical summary of the potential effect of aryl-hydrocarbon receptor activation on multipotent mesenchymal stromal cell function. (wjgnet.com)

These adult stem cells have demonstrated promising therapeutic benefits with less concerns about ethics, regulation, and tumor formation, as compared to embryonic stem cells. (biomedcentral.com)
Adult stem cells are found in a few select locations in the body, known as niches, such as those in the bone marrow or gonads. (wikipedia.org)
Li, L. & Clevers, H. Coexistence of quiescent and active adult stem cells in mammals. (nature.com)
Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
Studies of embryonic stem cells and adult stem cells are covered, along with research shedding light on the roles of these cells in regeneration and cancer. (cshlpress.com)
Scientists have isolated adult stem cells in human hair follicles. (loe.org)
HACK: In their search for regenerative cell therapies that might some day cure Parkinson's, Alzheimer's, and other diseases, scientists have studied adult stem cells found in the hair follicles of mice. (loe.org)
The key functions of adult stem cells are to maintain and repair the specific tissues where they reside (e.g. skin or blood). (bvsalud.org)

Within the neural stem cell niche of the mouse olfactory epithelium, we identified a combination of extracellular matrix (ECM) receptors that regulate adhesion and mitosis in non-neural stem cells [intercellular adhesion molecule-1 (ICAM-1), β 1 , β 4 , and α-1, -3, and -6 integrins] and on horizontal basal cells (HBCs), candidate olfactory neuro-epithelial progenitors. (syr.edu)
Polyclonal T-cell reconstitution of X-SCID recipients after in utero transplantation of lymphoid-primed multipotent progenitors. (ox.ac.uk)
Herein, we demonstrate in a congenic setting that recently identified lymphoid-primed multipotent progenitors are superior to hematopoietic stem cells in providing rapid lymphoid reconstitution after IUHCT of X-SCID recipients, and sustain in the long-term B cells, polyclonal T cells, as well as short-lived B-cell progenitors and thymic T-cell precursors. (ox.ac.uk)

Mesodermal lineage induction of PLA cells and clones resulted in the expression of multiple lineage-specific genes and proteins. (livingroom.health)
They are the earliest type of cell in a cell lineage. (wikipedia.org)
This population of small cells includes a CD45-negative fraction that lacks hematopoietic stem cell and lineage markers and resides in the renal interstitial space. (tau.ac.il)
In tissue recombination models, only basal cells reconstitute a complete prostate gland, yet murine lineage-tracing experiments show that luminal cells generate basal cells . (bvsalud.org)

More recently, a unique set of microRNA have been shown to be associated with embryonic stem cells [43-48]. (buyresearchchemicalss.net)
Somatic cell nuclear transfer is a cloning method that can be used to create a cloned embryo for the use of its embryonic stem cells in stem cell therapy. (wikipedia.org)
By 1998, human embryonic stem cells were first isolated by American biologist James Thomson, which made it possible to have new transplantation methods or various cell types for testing new treatments. (wikipedia.org)
Embryonic stem cells, which must be extracted from embryos three to five days old (known as blastocysts, which contain only about 150 cells at this point of development). (articlecity.com)
They have many of the positive characteristics of embryonic stem cells while sourcing material is far less challenging, since postpartum tissue can be used. (articlecity.com)
These multipotent stem cells contain certain proteins - called markers - that have previously been found only in embryonic stem cells. (loe.org)
Although stem cells from human hair follicles are much harder to come by, and less versatile than embryonic stem cells, they're less controversial in today's political and religious debates over the ethics of stem cell research. (loe.org)

To deliver on that promise, our laboratory is collaborating with the laboratories of Markus Grompe, M.D., OHSU, and Gordon Keller, Ph.D., University Health Network, Toronto, Canada, in the development and characterization of monoclonal antibodies directed against cell surface molecules expressed by pancreatic cell subsets as well as pancreatic progenitor cells derived from pluripotent stem cells. (ohsu.edu)

However, obtaining a safe, reliable and sufficient source of stem cell for such treatment is a major limitation and challenge in tissue engineering. (ca.gov)
Pluripotent and/or multipotent stem cell-based therapeutics are a vital component of tissue engineering and regenerative medicine. (ca.gov)
Cord blood-derived hematopoietic stem cells have been used in the clinic for more than 25years, and right now another source of neonatal stem cells, cord tissue, comes into spotlight.A part of umbilical cord, called Wharton's jelly, is an abundant source of mesenchymal stemcells which hold promise for regenerative medicine. (ginekologiaipoloznictwo.com)
To confirm whether adipose tissue contains stem cells, the PLA population and multiple clonal isolates were analyzed using several molecular and biochemical approaches. (livingroom.health)
Stem cell therapy is a promising approach to promote neural tissue regeneration. (biomedcentral.com)
Tissue engineering and cell therapy approaches aim to take advantage of the repopulating ability and plasticity of multipotent stem cells to regenerate lost or diseased tissue. (tau.ac.il)
By examining the process of stem cells turning into cells in bones, heart muscle, nerves, and other organs and tissue, researchers can better understand how diseases and conditions develop. (globenewswire.com)
Stem cells have the potential to be grown to become new tissue for use in transplant and regenerative medicine. (globenewswire.com)
New areas of study include the effectiveness of using human stem cells that have been programmed into tissue-specific cells to test new drugs. (globenewswire.com)
Cell and tissue banking applications will account for the leading share of the market by 2020. (globenewswire.com)
Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)
With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)
Current research is focused on growing a wide range of new tissue from stem cells, including muscle, blood, brain, and cartilage cells. (articlecity.com)
Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
Our limited understanding of the complex cellular composition, anatomy, and function of this tissue has slowed the establishment of effective tissue regenerative (repair) and/or cell replacement (transplantation) therapies. (ohsu.edu)
Our laboratory, in collaboration with the laboratory of Jeffrey Whitsett, M.D., at the Cincinnati Children's Hospital, is generating and characterizing a panel of monoclonal antibodies targeting cell-surface markers on different subsets of pulmonary cells involved in the process of pulmonary tissue regeneration. (ohsu.edu)
He calls it "multipotent," because it can transform into many kinds of tissue. (loe.org)
Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)
Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
The applicant is responsible for a brain tumor tissue biobank which provides the basis for the studies on human GBM tumor cells and stroma. (lu.se)
Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. (medscape.com)
Normal proliferative tissues contain multipotent stem cells (SCs) that drive tissue turnover. (medscape.com)
By contrast, transit amplifying cells and differentiated cells are incapable of self-renewal and tissue regeneration. (medscape.com)
Tissue-specific stem cells (also known as Somatic Stem Cells) that appear during fetal development and remain in the body throughout life. (bvsalud.org)

Flow cytometry and immunocytochemistry confirmed the mesenchymal stem cell phenotype. (uzh.ch)
In addition to mesodermal capacity, PLA cells and clones differentiated into putative neurogenic cells, exhibiting a neuronal-like morphology and expressing several proteins consistent with the neuronal phenotype. (livingroom.health)
Finally, when injected directly into the renal parenchyma, shortly after ischemic/reperfusion injury, renal Sca-1 + Lin - cells, derived from ROSA26 reporter mice, adopt a tubular phenotype and potentially could contribute to kidney repair. (tau.ac.il)
These data define a unique phenotype for adult kidney-derived cells, which have potential as stem cells and may contribute to the regeneration of injured kidneys. (tau.ac.il)

Notably, FReP cells regenerate muscle and bone tissues but do not generate teratomas in vivo. (ca.gov)
Whereas both A and B types of glucose-6-phosphate dehydrogenase were found in nonhematopoietic tissues, only a single-enzyme type was found in the granulocytes, red cells, and platelets from each patient. (ashpublications.org)
Cell-based therapy using systemic infusion of GMSC in experimental colitis significantly ameliorated both clinical and histopathological severity of the colonic inflammation, restored the injured gastrointestinal mucosal tissues, reversed diarrhea and weight loss, and suppressed the overall disease activity in mice. (ca.gov)
Because bone marrow-derived stromal cells (BMSCs) are able to generate many cell types, they are envisioned as source of regenerative cells to repair numerous tissues, including bone, cartilage, and ligaments. (upc.edu)
Over the last years, dentistry has been exploring the potential application of stem cells from different origins in the regeneration of oral tissues that were lost or damaged by disease or trauma 1 . (bvsalud.org)
According to the cancer stem cell (CSC) concept, the cellular hierarchy in normal tissues is preserved in a number of hematological and solid malignancies. (medscape.com)

To understand the etiology of osteopetrosis, it is very essential to understand the bone-remodeling cycle and the cell biology of osteoclasts. (medscape.com)
In 1999, Baron clearly and concisely reviewed the cell biology of the bone remodeling. (medscape.com)
International Journal of Biochemistry & Cell Biology, 40 , 2335-2340. (springer.com)
Natural killer cell biology: An update and future directions. (springer.com)
Based on presentations by world-renowned investigators at the 73rd annual Cold Spring Harbor Symposium on Quantitative Biology, this volume reviews the latest advances in research on the control and regulation of stem cells. (cshlpress.com)

As of 2016[update], the only established medical therapy using stem cells is hematopoietic stem cell transplantation, first performed in 1958 by French oncologist Georges Mathé. (wikipedia.org)
Although successful in utero hematopoietic cell transplantation (IUHCT) of X-linked severe combined immune deficiency (X-SCID) with enriched stem and progenitor cells was achieved more than a decade ago, it remains applied only in rare cases. (ox.ac.uk)
For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
Treatment is often supportive, but Janus kinase 2 (JAK2) inhibitors, such as ruxolitinib , fedratinib , or pacritnib, may decrease symptoms and stem cell transplantation may be curative. (msdmanuals.com)

abstract = "Stem cells of adult regenerative organs share a common goal but few established conserved mechanisms. (syr.edu)

In 2003 he became Director of the OHSU Hematopoietic Cell Processing Laboratory, and in 2004 he became a member of the Oregon Stem Cell Center, where he is Director of the Center's monoclonal antibody and flow cytometry core facilities and conducts independent research. (ohsu.edu)
We prospectively identified, and isolated by flow cytometry, adult mouse lateral ventricle subventricular zone (SVZ) NICs as Glast mid EGFR high PlexinB2 high CD24 −/low O4/PSA-NCAM −/low Ter119/CD45 − (GEPCOT) cells. (elifesciences.org)
have used a technique called flow cytometry to identify and isolate neural stem cells and neurosphere-forming cells directly from the subventricular zone. (elifesciences.org)
Cells were tested for the expression of surface markers and for ALDH1 enzyme activity, by flow cytometry. (bvsalud.org)
Results: The isolated cells showed high expression of CD44 (98.8%), CD73 (100%), and CD90 (97.2%), and moderate expression of STRO-1 (18.4%) and ALDH1 (16.2%), by flow cytometry. (bvsalud.org)

In primary myelofibrosis, nucleated red blood cells (normoblasts) and myelocytes are released into the circulation (leukoerythroblastosis) when there is extramedullary hematopoiesis (ie, non-marrow organs have taken over blood cell production because of the fibrosed marrow). (msdmanuals.com)

The self renewal capacity of MSC, thought to be limited, is known to be enhanced under specific culture conditions such as serum concentration, cell seeding density, and inclusion of growth factors such as FGF-2 [9-11]. (buyresearchchemicalss.net)
MVSCs have a self-renewal ability and express markers of neural crest stem cells (NCSCs) such as Sox10 and Sox17. (biomedcentral.com)
In that same year, Siminovitch was a lead investigator for studies that found colony-forming cells were capable of self-renewal, which is a key defining property of stem cells that Till and McCulloch had theorized. (wikipedia.org)
SCs are not only capable of maintaining themselves (self-renewal) but also give rise to a proliferative 'transit amplifying' compartment and ultimately to postmitotic differentiated cells with specialized functions. (medscape.com)

Induced pluripotent stem cells (iPSCs) hold great potential for the modelling of these diseases or as potential therapeutic agents. (nature.com)
These were termed induced pluripotent stem cells (iPSCs). (wikipedia.org)
By using appropriate promoter combinations and shielding the LVs with the Is2 insulator, we have constructed the Lent-On-Plus Tet-On system that achieved efficient transgene regulation in human multipotent and pluripotent stem cells. (nih.gov)
To our knowledge, Lent-On-Plus is the first all-in-one vector system that tightly regulates transgene expression in bulk populations of human pluripotent stem cells and its progeny. (nih.gov)
Induced pluripotent stem cells, which harness the power of genetic reprogramming - basically, the altering of a cell's DNA - to change the course of cellular development. (articlecity.com)

Preliminary studies have recently identified a putative stem cell population within the adipose stromal compartment. (livingroom.health)
Several types of stem cells have been explored for peripheral nerve regeneration (PNR), including umbilical cord blood stem cells, bone marrow-derived stem cells, adipose-derived stem cells, and neural stem cells [ 5 ]. (biomedcentral.com)

Reprogramming of human fibroblasts into multipotent cells with a single ECM proteoglycan, fibromodulin. (ca.gov)
We report here a technique using a single molecule, recombinant human fibromodulin protein (FMOD), to reprogram human fibroblasts into multipotent cells. (ca.gov)
They include endothelial cells, fibroblasts, and keratinocytes. (frontiersin.org)
These primary myelofibrosis progeny cells stimulate bone marrow fibroblasts (which are not part of the neoplastic transformation) to secrete excessive collagen. (msdmanuals.com)

The proposed tolerance of the fetal immune system to foreign human leukocyte antigen early in gestation, a main rationale behind IUHCT, has recently been challenged by evidence for a considerable immune barrier against in utero transplanted allogeneic bone marrow cells. (ox.ac.uk)
We further provide evidence for IUHCT of hematopoietic stem cells giving superior B- and T-cell reconstitution in fetal X-SCID recipients compared with neonatal and adolescent recipients. (ox.ac.uk)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
Células madre de un tejido específico (también denominadas células madre somáticas) que aparecen durante el desarrollo fetal y permanecen en el cuerpo durante toda la vida. (bvsalud.org)

They discovered the blood-forming stem cell, the hematopoietic stem cell (HSC), through their pioneering work in mice. (wikipedia.org)
McCulloch and Till began a series of experiments in which bone marrow cells were injected into irradiated mice. (wikipedia.org)
They observed lumps in the spleens of the mice that were linearly proportional to the number of bone marrow cells injected. (wikipedia.org)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. (frontiersin.org)
On the other hand, the "exosome vesicle " is an extracellular particle released from the endosomal compartment of most eukaryotic cells. (frontiersin.org)
The exosome vesicles are a type of extracellular vesicles (EV), which are defined as lipid-bilayer spheroid structures, without replicating capacity, that are released from cells, including both prokaryotes and eukaryotes. (frontiersin.org)

In the present study, we aim the generation of Tet-On all-in-one lentiviral vectors (LVs) that tightly regulate transgene expression in human stem cells using the original TetR repressor. (nih.gov)

First neurological patients currently under-go stem cell treatment as experimental therapy after individual bioethical committee approval. (ginekologiaipoloznictwo.com)
Stem Cell Educator therapy slowly passes lymphocytes separated from a patient's blood over immobilized cord blood stem cells (CBSC) from healthy donors. (klb.lt)
This therapy reversed autoimmunity and promoted regeneration of islet ß cells, wich resulted decreasion of the median daily dose of of insuli n. (klb.lt)
The first therapy using stem cells was a bone marrow transplant performed by French oncologist Georges Mathé in 1958 on five workers at the Vinča Nuclear Institute in Yugoslavia who had been affected by a criticality accident. (wikipedia.org)
Technological advancements in cell therapy and an increased emphasis on the development of personalized medicines are further contributing to market growth. (globenewswire.com)
A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
Understanding of molecular mechanisms in natural killer cell therapy. (springer.com)
2014). Human NK cells: From surface receptors to the therapy of leukemias and solid tumors. (springer.com)
However, the supply of donor-derived islets will never meet the needs of the large number of patients who could potentially benefit from this cell-based therapy. (ohsu.edu)
However, the standardization of the manufacturing process of MSC-based cell therapy medicinal products in compliance with the requirements of the local authorities is obligatory and will allow us to obtain the necessary permits for product administration according to its intended use. (mdpi.com)
Mesenchymal stromal cells: a novel therapy for the treatment of chronic obstructive pulmonary disease? (bmj.com)
Mora MV, Ibán MAR, Heredia JD, Laakso RB, Cuéllar R, Arranz MG. Stem cell therapy in the management of shoulder rotator cuff disorders. (wjgnet.com)

The disorder has some clinical and laboratory features that resemble those seen in the clonal multipotent stem cell disorders chronic myelogenous leukemia, polycythemia vera, and agnogenic myeloid metaplasia. (ashpublications.org)
These data indicate that the disorders in these three patients are clonal and involve multipotent stem cells. (ashpublications.org)
Overview of Myeloproliferative Neoplasms Myeloproliferative neoplasms are clonal proliferations of bone marrow stem cells, which can manifest as an increased number of platelets, red blood cells (RBCs), or white blood cells (WBCs). (msdmanuals.com)

http://dx.doi.org/10.1016/j.jaci.2014.02.015 epithelial cells and airway smooth muscle cells. (cdc.gov)

The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)

Most of the conflicting data might arise from the presence of multiple subtypes of cells with heterogeneous morpho functional features within the MSC cultures. (unipi.it)
These various culture methods may be a major source of the heterogeneous nature of the MSC cell population. (buyresearchchemicalss.net)

The principle challenge today is to produce enough regenerative cells through a simple, consistent process that bypasses ethical and immunological barriers and avoids genome alteration for in vivo skeletal muscle and bone construction without causing tumor formation. (ca.gov)
Since FMOD reprogramming clearly involves neither oncogenes nor genome integrative approaches and FReP cells do not generate teratoma in vivo, FMOD reprogramming fulfills the requirements of the FDA to produce safe multipotent cells without known undesirable side effects. (ca.gov)
Here we report that hiPSC can, in a highly autonomous manner, recapitulate spatiotemporally each of the main steps of retinal development observed in vivo and form three-dimensional retinal cups that contain all major retinal cell types arranged in their proper layers. (nature.com)
In vivo therapeutic effects, including functional recovery and cell fate, were investigated. (biomedcentral.com)
In Vivo experiments were performed to determine single cell interactions in the chick embryo chorioallantoic membrane (CAM). (mst.edu)
In vivo, hMSC rolled on and adhered to arterioles in the chick embryo CAM, whereas control melanoma cells embolized. (mst.edu)
Shaping of natural killer cell antitumor activity by ex vivo cultivation. (springer.com)
GMSC can function as an immunomodulatory and anti-inflammatory component of the immune system in vivo and is a promising cell source for cell-based treatment in experimental inflammatory diseases. (ca.gov)
Our study suggests that both OMSCs and host cells contribute to bone formation in vivo. (ca.gov)
Although MSC EVs show great promise for regenerative medicine applications, insights into their uptake mechanisms by different target cells and the ability to control MSC EV properties for defined function in vivo have remained elusive knowledge gaps. (frontiersin.org)
Pre-GEPCOT cells could not form neurospheres but expressed the stem cell markers Slc1a3-CreER T , GFAP-CreER T2 , Sox2 CreERT2 , and Gli1 CreERT2 and were long-lived in vivo. (elifesciences.org)

Identification of multipotent luminal progenitor cells in human prostate organoid cultures. (bvsalud.org)
The identification of undifferentiated pre-GEPCOT and GEPCOT cells will make it possible to directly study the properties of these cells inside the mouse brain, and to isolate live cells to test how they function. (elifesciences.org)

and 3) facilitate the purification of cell culture expanded cell populations for cell-based therapies. (ohsu.edu)
In this study, we present integrated quantitative proteome, transcriptome, and methylome analyses of hematopoietic stem cells (HSCs) and four multipotent progenitor (MPP) populations. (lu.se)
[ 2 ] In addition, some tumor types do not appear to adhere to the CSC concept at all [ 3 , 4 ] or may have multiple independent tumor-initiating cell populations. (medscape.com)

The potential application of stem cells in regenerative medicine is increasingly promising especially in defects that are difficult to be self -regenerate. (ca.gov)
The demand for stem cell manufacturing has also been rising as stem cells are being used to generate healthy cells and replace diseased cells or in the development of regenerative medicine. (globenewswire.com)
Researchers continue to advance knowledge on stem cells and their applications in transplant and regenerative medicine. (globenewswire.com)
Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)

Therefore, FMOD reprogramming provides an alternative strategy for engineering patient-specific multipotent cells for basic research and therapeutic application. (ca.gov)
Overall, single protein, FMOD-based, cell reprograming bypasses the risks of mutation, gene instability, and malignancy associated with genetically-modified iPS cells, and provides an alternative strategy for engineering patient-specific multipotent cells for basic research and therapeutic application. (ca.gov)
Understanding these regulatory mechanisms may help to design novel processes for production of specific therapeutic cells for transplant. (buyresearchchemicalss.net)
The functional motor recovery and cell fate in the regenerated nerve were investigated to understand the therapeutic benefit. (biomedcentral.com)
Progressing a cell-based therapeutic from pre-clinical stage production through later clinical stages and into commercial production requires scale-up, which is one of the chief drivers for the stem cell manufacturing market growth. (globenewswire.com)

Both these cell types are being modulated by effector T-cells (Teff). (biomedcentral.com)

Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. (hindawi.com)

Kim and Park, 2017 ) and make HMSCs attractive cells for regenerative therapies. (frontiersin.org)

In addition, these cells are enriched for β1-integrin, are cytokeratin negative, and show minimal expression of surface markers that typically are found on bone marrow-derived mesenchymal stem cells. (tau.ac.il)
term pre-GEPCOT cells (based on an acronym of the markers used to isolate the cells), were long-lived and quiescent, but they lacked the ability to form colonies in culture. (elifesciences.org)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

We postulated that MVSCs may benefit PNR by differentiating into neural and/or vascular cell types in the regenerated nerve. (biomedcentral.com)
Neurosphere formation is commonly used as a surrogate for neural stem cell (NSC) function but the relationship between neurosphere-initiating cells (NICs) and NSCs remains unclear. (elifesciences.org)
Neurons that arise in the adult nervous system originate from neural stem cells and neural progenitor cells. (elifesciences.org)
Neural stem cells have long lives, much of which they spend in a quiescent state. (elifesciences.org)
Consequently, neural stem and progenitor cells have usually been studied retrospectively, based on their ability to form colonies in laboratory cell cultures. (elifesciences.org)
A region of the brain called the subventricular zone contains both neural stem cells and neural progenitor cells, and is one of only two regions of the brain where neural stem cells are found in adult mammals. (elifesciences.org)
However, the exact relationship between neural stem cells and neurosphere-forming cells has been uncertain. (elifesciences.org)
The neural stem cells, which Mich, Signer et al. (elifesciences.org)
Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres. (lu.se)
Enhanced expression of MycN/CIP2A drives neural crest toward a neural stem cell-like fate: Implications for priming of neuroblastoma. (lu.se)

2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)

An understanding of the architecture and behavior of transcriptional networks should lend insight into how the huge number of potential gene expression programs is constrained and facilitates efforts to direct or redirect cell fate. (ox.ac.uk)

Recently, the importance of epigenetic regulation via microRNA in controlling stem cell activities has been recognized [31-37]. (buyresearchchemicalss.net)

Background: There have been conflicting observations regarding the receptors utilized by human multipotent mesenchymal bone marrow stromal cells (hMSC) to adhere to endothelial cells (EC). (mst.edu)

However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. (medscape.com)
The CSC is defined as a cancer cell with self-renewing capacity that can regenerate the original tumor in all its differentiated heterogeneity. (medscape.com)

This allows for a neuroprotection of neurons and other cell types. (biomedcentral.com)

Anatomy19

Organisms2

Chemicals and Drugs10

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Phenomena and Processes14

Progenitor cell4

Differentiation25

Isolation8

Proliferation10

Differentiate20

Vitro14

MSCs19

Mesenchymal stromal cell2

Adult stem8

Progenitors3

Lineage4

Embryonic stem7

Characterization1

Tissue24

Phenotype4

Tissues6

Biology5

Transplantation4

Abstract1

Flow Cytometry5

Hematopoiesis1

Self-renewal4

Pluripotent Stem5

Adipose2

Fibroblasts4

Fetal4

Mice4

Extracellular3

Vectors1

Therapy12

Clonal3

Epithelial1

Immunomodulatory properties1

Heterogeneous2

Vivo11

Identification2

Populations3

Regenerative medicine4

Therapeutic5

Modulated by effector T-cel1

Recurrence1

20171

Markers4

Neural10

Humans1

Gene expression programs1

Regulation1

Endothelial1

Tumor3

Neurons1