IsotypeImmunogenReactivitySpecificityMAbsProteinsConjugateMurine monoclonal antibodiesDatasheetAntigen-specificChimericELISAImmunoglobulinAntigensAscitesConcentrationHumanRecombinant proteinBispecificEndogenousSpeciesDetectsProteinCatalogRecognizeEpitopesConjugationAssaysLocalizationVitroConjugatesInhibitoryTypeTherapeuticallyKarlanInhibitionPrimaryDetectionBiologicalClinicalEpitopeCancerDilutionActinFluoresceinSpecific
Isotype2
- Monoclonal Anti-MUC1 (mouse IgG1 isotype) is derived from the hybridoma 1D1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with semi-purified MUC1 from pleural effusion of a breast cancer patient. (sigmaaldrich.com)
- Search, find, compare suppliers for Mouse IgG3 Isotype Control products. (linscottsdirectory.com)
Immunogen2
- Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. (biocheminfo.org)
- The immunogen used for antibody production was purified actin protein from rabbit skeletal muscle. (cytoskeleton.com)
Reactivity3
- Aydintug K, Inzana TJ, Letonja T, Davis WC, Corbeil LB. Cross-reactivity of monoclonal antibodies to Escherichia coli J5 with heterologous gram-negative bacteria and extracted lipopolysaccharides. (linscottsdirectory.com)
- The antibody has been shown to recognize α-skeletal, α-cardiac, α-smooth muscle, β-cytoplasmic, γ-smooth muscle and γ- cytoplasmic actin isotypes (Fig. 1) and has broad species cross-reactivity. (cytoskeleton.com)
- This mouse monoclonal antibody to human glutathione S-transferase O1-1 is protein A & A-G affinity purified and shows no cross reactivity to other cytosolic GSTs. (oxfordbiomed.com)
Specificity6
- A panel of 11 murine monoclonal antibodies directed against dengue type 2 was evaluated for antigen specificity by dot immunobinding assay and Western blot analysis and for in vitro and in vivo biological activities. (ajtmh.org)
- Specificity These antibodies recognize gentamicin. (qedbio.com)
- Specificity These antibodies specifically recognize human serum alpha-1-antichymotrypsin. (qedbio.com)
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. (biosensis.com)
- In this study we have profiled a panel of mouse antihuman CD40 monoclonal antibodies (MoAbs), regarding their CD40 binding affinity and epitope-specificity relative to the CD40L binding in relation to their cellular activating potential. (lu.se)
- T1", "T2" and "T3") and form a visible line as the antibody-antigen-antibody gold particle complex with high degree of sensitivity and specificity. (who.int)
MAbs5
- The rationale of this study was to determine the myelotoxicity in nude mice of the α-emitter 211 At conjugated to monoclonal antibodies (mAbs) and to compare the effect with an electron emitter, 99m Tc, and external irradiation from a 60 Co source, for estimation of the relative biological effectiveness (RBE). (snmjournals.org)
- Coupled to monoclonal antibodies (mAbs), or fragments of antibodies, α-particle emitters provide a promising tool in treating different kinds of disseminated cancers. (snmjournals.org)
- Monoclonal antibodies (MAbs) that recognize TDI bound proteins would be valuable tools/reagents, both in exposure monitoring and in TDI-induced asthma research. (cdc.gov)
- In this work, we evaluated a panel of 16 anti-BoNT/A monoclonal antibodies (mAbs) for their ability to inhibit the in vitro activity of BoNT/A1, /A2, and /A3 complex as well as the recombinant LC of A1. (cdc.gov)
- The need for high-affinity, SARS-CoV-2-specific monoclonal antibodies (mAbs) is critical in the face of the global COVID-19 pandemic, as such reagents can have important diagnostic, research, and therapeutic applications. (cdc.gov)
Proteins8
- FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. (jacksonimmuno.com)
- Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. (vwr.com)
- A survey of various species by immunoblot analysis with several antibodies revealed little if any epitope relatedness among microsomal proteins from rats, mice, rabbits, hamsters, squirrel monkeys, guinea pigs, or humans. (nih.gov)
- FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. (anobase.org)
- Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten. (anobase.org)
- This antibody specifically targets the (His)6 tag of proteins and allows their detection by ELISA, Western Blot and Immuno-precipitation. (moleculardepot.com)
- Monoclonal antibodies against toluene diisocyanate haptenated proteins from vapor-exposed mice. (cdc.gov)
- These antibodies may be useful tools for the isolation of endogenous diisocyanate-modified proteins after natural or experimental exposures and for characterization of the toxicity of specific dNCOs. (cdc.gov)
Conjugate1
- Mouse monoclonal antibodies specific to Zaire Ebola virus GP, NP and VP40 - colloid gold conjugate reacts with the Zaire Ebola virus in the specimen. (who.int)
Murine monoclonal antibodies2
- Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
- Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)
Datasheet1
- If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
Antigen-specific3
- Several antibodies were antigen-specific, others reacted with additional members of the 2C subfamily, and other monoclonal antibodies recognized cytochromes P450 from the 2E, 2B, 2A, and 1A subfamilies. (nih.gov)
- Much of the work behind production of monoclonal antibodies is rooted in the production of hybridomas, which involves identifying antigen-specific plasma/plasmablast cells that produce antibodies specific to an antigen of interest and fusing these cells with myeloma cells. (wikipedia.org)
- Immunophenotyping relies on detecting specific antigenic determinants on the surface of WBCs by antigen-specific monoclonal antibodies that have been labeled with a fluorescent dye or fluorochrome, such as phycoerythrin (PE) or fluorescein isothiocyanate (FITC). (cdc.gov)
Chimeric2
- The Committee was informed of progress in research into chimeric chimpanzee/human monoclonal antibodies. (who.int)
- If the mouse gene for the whole variable region is spliced next to the human constant region, the product is termed "chimeric. (msdmanuals.com)
ELISA3
- Application Instructions These antibodies have been qualified for use in ELISA to detect and gentamicin. (qedbio.com)
- The antibodies secreted by the different clones are then assayed for their ability to bind to the antigen (with a test such as ELISA or antigen microarray assay) or immuno-dot blot. (wikipedia.org)
- A participant was considered seropositive if they tested positive for SARS-CoV-2 immunoglobulin G (IgG) antibodies on the Abbott Architect SARS-CoV-2 IgG ELISA and the EUROIMMUN SARS-CoV-2 ELISA in a sequential testing algorithm. (cdc.gov)
Immunoglobulin2
- Then the DNA for some or all of the variable portion of the mouse antibody is merged with DNA for human immunoglobulin. (msdmanuals.com)
- Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)
Antigens3
- In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. (roche.com)
- Rickettsia monoclonals have been produced against both protein and LPS (lipopolysaccharide) antigens of several species over the years. (fullerlabs.com)
- In 1975, Georges Köhler and César Milstein succeeded in making fusions of myeloma cell lines with B cells to create hybridomas that could produce antibodies, specific to known antigens and that were immortalized. (wikipedia.org)
Ascites1
- The IgG fraction was purified from mouse ascites by chromatographic procedures not involving affinity purification. (jacksonimmuno.com)
Concentration2
- For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. (origene.com)
- Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. (biosensis.com)
Human9
- Immunohistochemistry analysis in human placenta and kidney tissues using AMAb91497 antibody. (atlasantibodies.com)
- IGF-BP53 antibody LS-C7524 is an unconjugated mouse monoclonal antibody to human IGF-BP53 (IGFBP2). (lsbio.com)
- This antibody is recommended for detection of actin in a broad spectrum of species such as human, mouse, rat, and bovine. (cytoskeleton.com)
- This antibody was raised against full length recombinant human SNAI1 protein (snail homolog 1 (Drosophila). (thermofisher.com)
- We believe that this analysis of several mouse anti-CD40 antibodies can be used to develop strategies for producing new human anti-CD40 antibodies that can more effectively induce or block B-cell proliferation. (lu.se)
- 324 In 1973, Jerrold Schwaber described the production of monoclonal antibodies using human-mouse hybrid cells. (wikipedia.org)
- The University of Massachusetts Medical School's MassBiologics has developed a human monoclonal antibody designed to be used as pre-exposure prophylaxis (PrEP) for Lyme disease. (cdc.gov)
- Immunohisto- have been used for positive selection of NSCs from em- chemistry on human embryonic central nervous system bryonic mice (Nagato et al. (lu.se)
- To minimize the problems due to use of pure mouse antibody, researchers have used recombinant DNA techniques to create monoclonal antibodies that are part human and part mouse. (msdmanuals.com)
Recombinant protein1
- Western blot analysis of BCMA: Lane 1: MW marker, Lane 2: Mouse BCMA Recombinant Protein. (enzolifesciences.com)
Bispecific1
- Bispecific monoclonal antibodies can also be engineered, by increasing the therapeutic targets of one monoclonal antibody to two epitopes. (wikipedia.org)
Endogenous2
- The antibody detects endogenous Collagen II protein. (biocheminfo.org)
- HDAC3 Mouse Monoclonal Antibody detects endogenous levels of HDAC3 protein. (ebiocell.com)
Species1
- Flavivirus antibodies were detected in 13 species, and one Australasian gannet ( Morus serrator ) from one site was positive for antibodies to Ross River virus. (who.int)
Detects1
- Monoclonal anti SFG LPS detects all spotted fever Rickettsia, while anti typhus group LPS reacts with both R. typhi and R. prowazekii . (fullerlabs.com)
Protein2
- AAN02 is a mouse monoclonal antibody against actin protein. (cytoskeleton.com)
- avenue of research has been the development of protein-based "point-of-care" diagnostic assays for antigen and antibody detection. (who.int)
Catalog2
- The following product was used in this experiment: MURF3 Monoclonal Antibody (OTI7F8) from Thermo Fisher Scientific, catalog # MA5-26633, RRID AB_2724760. (thermofisher.com)
- The following product was used in this experiment: Snail Recombinant Mouse Monoclonal Antibody, AbFlex® from Thermo Fisher Scientific, catalog # 91255, RRID AB_2793821. (thermofisher.com)
Recognize3
- This antibody can recognize all the actins. (caslab.com)
- Because different antibodies recognize different fragments, no standard currently exists for osteocalcin assays. (medscape.com)
- Antibodies that recognize both the large amino-terminal mid-molecule fragment and the intact molecule appear to provide superior clinical information. (medscape.com)
Epitopes3
- The protective monoclonal antibodies were directed against viral determinants that fell into at least three spatially separate families of epitopes on E-glycoprotein, the antigenicities of which were preserved after heat/detergent denaturation. (ajtmh.org)
- A minimum of seven spatially distinct epitopes on cytochrome P450h were defined by the panel of antibodies. (nih.gov)
- In contrast, polyclonal antibodies bind to multiple epitopes and are usually made by several different antibody-secreting plasma cell lineages. (wikipedia.org)
Conjugation1
- To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (origene.com)
Assays1
- Particle-enhanced assays were based on the reaction between a soluble analyte and the corresponding antigen or antibody bound to polystyrene particles. (cdc.gov)
Localization2
- The localization of 111In-labelled MA5 monoclonal antibody, reactive with a breast tumor associated antigen, was studied in 17 patients. (mcmaster.ca)
- Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. (anobase.org)
Vitro1
- This antibody is intended for in vitro diagnostic (IVD) use. (roche.com)
Conjugates2
- IgG fraction Monoclonal Mouse Anti-Digoxin may be used either as direct conjugates, or for more sensitivity, they can be used unconjugated followed by a conjugated anti-mouse IgG (H+L) for signal enhancement . (jacksonimmuno.com)
- This underpinned the concept of monoclonal antibodies and monoclonal drug conjugates. (wikipedia.org)
Inhibitory3
- These inhibitory antibodies gave similar results when benzphetamine N-demethylation was evaluated in the reconstituted system. (nih.gov)
- The inhibitory antibodies were then used to assess the role of cytochrome P450h in microsomal benzphetamine N-demethylation, since this isozyme exhibits high catalytic activity for this substrate. (nih.gov)
- By the 1990s research was making progress in using monoclonal antibodies therapeutically, and in 2018, James P. Allison and Tasuku Honjo received the Nobel Prize in Physiology or Medicine for their discovery of cancer therapy by inhibition of negative immune regulation, using monoclonal antibodies that prevent inhibitory linkages. (wikipedia.org)
Type1
- Recently, the monoclonal antibody teplizumab (Tzield, Sanofi) was approved as a treament for delaying the onset of type 1 diabetes in people with autoantibodies that signify a preclinical stage of the condition. (medscape.com)
Therapeutically1
- Combinations of antibodies fully protected mice challenged with vaccinia virus and were also active therapeutically. (who.int)
Karlan1
- The MSDS of Anti-PCNA for Mouse is available from Karlan upon request. (dnamethsoc.com)
Inhibition2
- Although most of the antibodies were noninhibitory, greater than 70% inhibition of 2 alpha- and 16 alpha-hydroxylation of testosterone was observed with selected antibodies. (nih.gov)
- Only 20-25% inhibition of benzphetamine metabolism was attained in microsomal preparations from adult male rats, and the antibodies did not influence the microsomal catalytic activity of immature males or females or adult females. (nih.gov)
Primary2
- The primary antibody was detected with a 1:500 dilution of goat anti-mouse alexfluor-488 conjugated antibody. (cytoskeleton.com)
- en Ksp-Cadherin (MRQ-33) Mouse Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. (roche.com)
Detection3
- Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. (roche.com)
- Mouse monoclonal clone FL-D6 anti-FITC antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. (anobase.org)
- Sera from each period ( n = 185 and n = 693) were screened in an epitope blocking enzyme immunoassay for flavivirus antibody detection. (who.int)
Biological1
- This study investigates the myelotoxicity and determines a value of the relative biological effectiveness (RBE) of the α-emitter 211 At in nude mice in comparison with an electron emitter, 99m Tc, and external irradiation. (snmjournals.org)
Clinical2
- Monoclonal antibodies are being used on a clinical level for both the diagnosis and therapy of several diseases. (wikipedia.org)
- We have previously reported the effectiveness of antibody-capture to purify and concentrate BoNTs from complex matrices, such as clinical samples. (cdc.gov)
Epitope1
- Monoclonal antibodies can have monovalent affinity, binding only to the same epitope (the part of an antigen that is recognized by the antibody). (wikipedia.org)
Cancer2
- By the 1970s, lymphocytes producing a single antibody were known, in the form of multiple myeloma - a cancer affecting B-cells. (wikipedia.org)
- Colorectal cancer, the third most com- case to be made for a mass screening HRP rabbit/mouse for hMSH6 , with mon cancer in the world [1], is rare programme, preferably for individuals rabbit anti-mouse IgG, dilution 1:400, before the age of 40 years and shows a aged less than 50 years old. (who.int)
Dilution2
- The blot was probed with a 1:1000 dilution of anti-actin antibody. (cytoskeleton.com)
- Immunofluorescence staining using 1:500 dilution of anti-actin antibody in PBS is shown (green). (cytoskeleton.com)
Actin2
- Western blot of purified actin and cell extracts probed with anti-actin antibody. (cytoskeleton.com)
- Immunofluorescence images of mouse Swiss 3T3 cells stained with anti-actin antibody. (cytoskeleton.com)
Fluorescein1
- The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC). (anobase.org)
Specific6
- Immunofluorescence staining of SK-MEL-30 cells using the Anti-CDK2 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. (atlasantibodies.com)
- These abnormal antibodies or paraproteins were used to study the structure of antibodies, but it was not yet possible to produce identical antibodies specific to a given antigen. (wikipedia.org)
- Oocyte-specific, beta1 integrin conditional knockout mice allowed us to obtain mature eggs lacking. (lu.se)
- This study as a link to amplify between the pri- significant age-specific increase in in- was therefore conducted to identify mary antibody and the Envision step. (who.int)
- The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
- In the first season, testing for antibodies to specific alphaviruses was conducted on samples with sufficient sera ( n = 22). (who.int)