Morphology assays lymphocytes cell proliferation. Medical search. Frequent questions

For example, the authors have found that AM from young children are less suppressive for T-lymphocyte proliferation using in vitro functional assays 2 , but the developmental changes of relevant immune receptors could not be identified because of the limited numbers of cells recovered. (ersjournals.com)
When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)
This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
We also evaluated the impact of proinflammatory molecules TNFα and IFNγ on IL32 expression and dedifferentiation in melanoma cell lines in vitro. (biomedcentral.com)
The virus infects the antibody producing B lymphocytes in vitro conditions. (scienceofhealthy.com)
The immunization of cells in vitro by this EBV virus is specifically termed as transformation. (scienceofhealthy.com)
10-20% of the cells of this cell line undergo lysis in vitro conditions at regular intervals, releasing these EBV viruses in culture media. (scienceofhealthy.com)
Furthermore, we demonstrated that these automatically isolated cells bear proliferation and differentiation capacities comparable to manually isolated cells in vitro. (biomedcentral.com)
Previous in vitro studies have shown that the venom of the spider Phoneutria nigriventer (PnV) is a potential source of antineoplastic components with activity in glioblastoma (GB) cell lines. (nature.com)
A recent in vitro screening of the Phoneutria nigriventer spider venom (PnV) antitumor effects by our group has shown that the venom significantly affected glioblastoma cell lines. (nature.com)
Proteins encoded by the cloned ORF plasmids are expressed using a cell free in vitro transcription/ translation ("IVTT") system. (antigendiscovery.com)
ADI uses the same high-throughput cloning and cell-free in vitro transcription and translation methodology employed in the manufacture of protein microarrays (described here ). (antigendiscovery.com)
In vitro , UC-MSCs were capable of inhibiting proliferation of FLSs from RA patients, via IL-10, IDO and TGF-β1. (biomedcentral.com)
In conclusion, human UC-MSCs suppressed the various inflammatory effects of FLSs and T cells of RA in vitro , and attenuated the development of CIA in vivo , strongly suggesting that UC-MSCs might be a therapeutic strategy in RA. (biomedcentral.com)
This is discomforting, because genotoxic effects of the second generation standard Global System for Mobile Communication have been reported after exposure of human cells in vitro. (arasysperfector.com)
UMTS exposure may cause genetic alterations in some but not in all human cells in vitro. (arasysperfector.com)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)

Impairment in thymocyte numbers, development, proliferation, and apoptosis were observed for up to two weeks. (en-journal.org)
https://doi.org/ 10.3390/app122211314 the cell number and stimulated apoptosis. (deepdyve.com)
Many factors and components play a role in the cell cycle of K562 cells in terms of growth, cell differentiation, and apoptosis. (wikipedia.org)
The growth of these leukemic cells are controlled by either initiating cell differentiation or apoptosis to occur. (wikipedia.org)
These changes can also drive the leukemic cells to a state of stress, which allows for increased sensitivity of the cells to drugs that initiate apoptosis. (wikipedia.org)
Apoptosis is an important mechanism in regulating K562 cells and can be induced by the changes in the metabolic state of the cells. (wikipedia.org)
There are many different cellular components involved in the cycle of apoptosis such as BCR/ABL, Bcl-2, Bax protein, and cytochrome C. The tumor suppressor gene p53 is also important in the cell cycle regulation of K562 cells. (wikipedia.org)
This gene targets the cyclin-dependent kinase inhibitor, p21, and causes cell differentiation, cell cycle arrest in G1, and ultimately apoptosis. (wikipedia.org)
When the levels of these components are thrown off, they can either no longer inhibit apoptosis of the cancer cells, a role fulfilled by BCR/ABL, or they cause apoptosis to be induced, in the same vein as Bax and cytochrome C. These components are key in the mitochondria, and due to this, it has been supported that apoptosis uses the mitochondrial apoptosis pathway. (wikipedia.org)
Other methods being focused on in the regulation of K562 cells include therapeutic methods like polyphyllin D, which caused differentiation from the progenitor state to occur, and for apoptosis to begin. (wikipedia.org)
ATZ also induces morphological changes similar to apoptosis and initiates mitochondria-dependent cell death in several experimental models. (frontiersin.org)
J) Annexin V and (K) Ki-67 staining of CD33+ myeloid cells show apoptosis and proliferation, respectively. (eprf.ca)
Apoptosis was checked by acridine orange/ethidium bromine staining assay and by execution of Western blotting analysis. (ijpsonline.com)
Acridine orange/ethidium bromine staining assay revealed that piperine induced apoptosis in MCF-7 cells which was further investigated by Western blotting. (ijpsonline.com)
This revealed increased expression of B-cell lymphoma 2 associated X and reduced expression of B-cell lymphoma 2, indicating apoptosis induction by piperine. (ijpsonline.com)
It induced apoptosis, suppressed cell migration and invasion, blocked cell cycle and phosphatidylinositol 3-kinase/ protein kinase B signaling pathway. (ijpsonline.com)
Conclusion】 The PERP 1 gene had the function of promoting granulosa cell apoptosis,and regulating its expression was of great significance for follicular development. (chvm.net)
The mitochondrial mediated apoptosis and inflammatory markers were evaluated using immunoblotting assay. (sagepub.com)
MNU 47 mg/kg, i.v). Tadalafil was ad- apoptosis and also simultaneously affect cancer cell ministered from 7th to 110th day at the dose specified growth and adhesion, mitochondrial energy homeostasis, above. (sagepub.com)
In an ex vivo model KNK437 was used as an inhibition model assay of HSP70, showed dose-dependent inhibition of cell growth and burst formation unit erythroid (BFU-E) in PV and ET, increased apoptosis in the erythroid lineage, and decreased pJAK2 signaling, as well as a specific siRNA for HSP70. (biomedcentral.com)
Overexpression of HSP70 can provide a selective survival advantage to tumor cells in part due to its ability to inhibit multiple pathways of cell death, including both intrinsic and extrinsic apoptosis. (biomedcentral.com)
Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)

Mesenchymal stem cells (MSCs) are promising candidates for cell-based therapies. (biomedcentral.com)
mesenchymal stem cells are most commonly used because they are easy to obtain and present no ethical problems. (springer.com)
The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
Mouse anti Human CD44 antibody, clone F10-44-2 ( MCA89 ) used for the identification of mesenchymal stem cells in mixed bone marrow cell populations by flow cytometry. (bio-rad-antibodies.com)
Mesenchymal stem cells (MSCs) have been widely used in regenerative medicine and clinical therapy due to their capabilities of proliferation, differentiation, and immune regulation. (techscience.com)
Bone marrow mesenchymal stem cells (MSCs) could be effective in the treatment of several autoimmune diseases. (biomedcentral.com)
Mesenchymal stem cells (MSCs) are cells of stromal origin that can exert profound immunosuppression by modulating T and B cell proliferation and differentiation, dendritic cell maturation and NK activity. (biomedcentral.com)
Bone marrow mesenchymal stem cells (BMSCs) have potential therapeutic benefits for the treatment of endometrial diseases and injury. (biologists.com)

In children, the description of developmental changes in immune receptor expression on alveolar macrophages (AM) has been limited by the small number of cells recovered by bronchoalveolar lavage (BAL). (ersjournals.com)
It is concluded that laser scanning cytometry is suited to the study of immune receptor expression from small numbers of paediatric alveolar macrophages, when CD68 is used for cell identification. (ersjournals.com)
The change in phenotype induces a decrease in the growth rate and leads the K562 cells to the terminal path of becoming mature erythroids, monocytes, and mature macrophages. (wikipedia.org)
Dendritic cells (DCs), 4 monocytes/macrophages, and B cells have been identified as major professional APCs ( 1 , 2 ). (aai.org)
Interestingly, when encapsulated cells were co-cultured with THP-1 macrophages, co-cultures exhibited TNF-α production enhancement. (springer.com)
Interleukin 32 (IL32) is produced by T cells, NK cells and monocytes/macrophages, but also by a subset of melanoma cells. (biomedcentral.com)
Histone adsorption on the surface of carbon particles significantly stimulates their ingestion by rat peritoneal macrophages, hamster kidney fibroblasts, and mouse L-cells [ 861 ], and the adsorption of polycyclic aromatic hydrocarbons (PAHs) and their nonpolar metabolites on the surface of carbon particles ingested by rat alveolar macrophages has been studied [ 862 ]. (nanomedicine.com)
These features may be responsible for the recruitment and activation of T cells, macrophages and dendritic cells, characterizing the stromal component of this lymphoma, and may point towards innate immunity and a tumor tolerogenic immune response in T-cell/histiocyte-rich large B-cell lymphoma. (haematologica.org)
PnV (100 µg/Kg) was administrated every 48 h (i.p.) for 14 days and several endpoints were evaluated: tumor growth and metabolism (by microPET/CT, using 18 F-FDG), tumor weight and volume, histopathology, blood analysis, percentage and profile of macrophages, neutrophils and NK cells isolated from the spleen (by flow cytometry) and the presence of macrophages (Iba-1 positive) within/surrounding the tumor. (nature.com)
Immunotherapy includes systemic administration of cytokines, adaptive transfer of activated T cells, NK cells, dendritic cells and macrophages. (healthcaremarts.com)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

We show that lower Differential Effects of Resveratrol on concentrations of RSV (1, 5, 10 M) had no effect on proliferation, viability or cell cycle progression in HECa10 and ARPE-19 Cells. (deepdyve.com)
In ARPE-19 cells, lower concentrations of RSV increased the rate of proliferation, while higher concentrations had no effect on proliferation and viability. (deepdyve.com)
The entire manufacturing process, the examination of cell number and viability of the final CP as well as the depletion of non-target cells were part of the validation process, which was performed in compliance with EU guidelines for GMP. (biomedcentral.com)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was executed for determination of effects of piperine on breast cancer cell viability. (ijpsonline.com)

The bone marrow- and adipose tissue-derived MSCs were cultured under human platelet lysate conditions and their biological characteristics evaluated for cell therapy (morphology, immunophenotype, colony-forming unit-fibroblast efficiency, proliferation capacity, potential for mesodermal differentiation, secreted proteins, and immunomodulatory effects). (biomedcentral.com)
Each clinicopathologic subtype can be further classified with respect to morphology, immunophenotype, and antigen markers. (medscape.com)
Acute myeloid leukemia has a number of subtypes and precursor neoplasms that are distinguished from each other by morphology, immunophenotype, cytochemistry, and genetic abnormalities (see also The 2016 World Health Organization [WHO] Classification of myeloid neoplasms ) all of which have important implications for prognosis and treatment. (msdmanuals.com)

Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. (mdpi.com)
In the tumor microenvironment, IL32 expression is highly correlated with genes related to T cell infiltration, and also positively correlates with high AXL/low MITF dedifferentiated gene signature. (biomedcentral.com)
Histopathological analysis revealed that liver injury, characterized by collagen deposition and inflammatory cell infiltration, occurred as early as 24 h of infection. (biomedcentral.com)

PBMCs include lymphocytes, monocytes, and DCs. (frontiersin.org)
K562 cells can spontaneously develop characteristics similar to early-stage erythrocytes, granulocytes and monocytes and are easily killed by natural killer cells as they lack the MHC complex required to inhibit NK activity. (wikipedia.org)
The venom increased monocytes, neutrophils and NK cells, and this effect was the opposite of that observed in the animals treated with MTX. (nature.com)

The peripheral blood mononuclear cells (PBMCs) have a round nucleus ( Delves, 2016 ). (frontiersin.org)

The animal tissue and serum samples were evaluated for the presence of antioxidant parameters and the cellular morphology was studied using carminic staining, haematoxylin staining and scanning electron microscopy followed by immunoblotting analysis. (sagepub.com)

The offset of these cellular components from their balance point causes morphological changes, which result in the K562 cells being arrested in the G2/M phase of the cell cycle. (wikipedia.org)
This arrest leads to "shrinkage, blebbing, nuclear fragmentation, chromatin condensing" and other morphological changes that cause the cell to program death at this point. (wikipedia.org)

The first report in 2007, on SAA-stimulated human coronary artery endothelial cells (HCAEC), exhibited a substantial and significantly higher induction of released IL-6 protein and mRNA levels as compared to HUVEC [ 17 ], as well as increased responsiveness to IL-1 β [ 18 ]. (hindawi.com)
G and H) Representative immunoblots show increased pSTAT5 and total STAT5 (G), and pERK (H) protein expression levels in NS/JMML CD33+ myeloid cells. (eprf.ca)
This study aimed to explore the effects of Acacia honey (AH) on corneal keratocytes morphology, proliferative capacity, cell cycle, gene and protein analyses. (blogspot.com)
Corneal keratocytes cultured in media supplemented with 0.025% AH showed an increase in proliferative capacity while retaining their morphology, gene and protein expressions with normal cell cycle. (blogspot.com)
Western blotting assay was implemented to check the levels of phosphatidylinositol 3-kinase and protein kinase B. Results revealed that piperine induced dose reliant cytotoxicity in MCF-7 human breast cancer cells. (ijpsonline.com)
Finally, Western blotting assay predicted constant expression of phosphatidylinositol 3-kinase and protein kinase B and reduced expression of phosphorylated phosphatidylinositol 3-kinase. (ijpsonline.com)
The sheathed vessels of the spleen contained proliferations of phagocytic cells containing intracytoplasmic protein. (susanclubb.com)
Workflows have been developed for the expansion of pathogen-specific CD4 or CD8 T cells expressing CD137, which can then be used in screens against pathogen protein libraries to identify those that specifically activate T cells (e.g. proliferation or cytokine secretion). (antigendiscovery.com)
Scaffolds' structure, cells morphology, and protein presence in the membranes were examined with a scanning electron microscope. (pan.pl)
Obtained results showed higher cell activity and protein content for PES scaffolds than for PLLA. (pan.pl)

Dendritic cells (DCs), which are the most important antigen-presenting cells (APCs) that send signals to the T cells, mainly participate in the pathogenesis of many diseases with immunoregulatory mechanisms, such as AR. (frontiersin.org)
Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)
ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)
Neither the B-lymphocyte antigen CD19 nor the T-cell surface glycoprotein CD3 gamma chain (hybridization with a dual probe showed an absence of the fusion (Physique S3A). (eprf.ca)
The infection of cells with this virus results in expression of EBV nuclear antigen and this immortalize the infected cells. (scienceofhealthy.com)
Antigen Discovery Inc., in collaboration with David Koelle's laboratory Laboratory at the University of Washington in Seattle, has developed a high-throughput screening methodology for identification of antigen-specific CD4 and CD8 T cell specificities in cellular samples. (antigendiscovery.com)
Expanded T cells are screened in stimulations with pathogen proteins in the presence of autologous PBMC as antigen-presenting cells (APC). (antigendiscovery.com)
A read-out assay such as 3 H-thymidine incorporation or cytokine secretion is used to identify the targets of antigen-specific responses. (antigendiscovery.com)

Cell differentiation is induced by the deacetylase activity in these "undifferentiated progenitor cells," which alters the phenotype and morphology of the K562 cells. (wikipedia.org)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
BC comprises of variant subtypes and those subtypes arise from basal progenitor or luminal progenitor cells undergoing different genetic mutations[ 2 ]. (ijpsonline.com)

Both Academic Editors: Joanna ARPE-19 and HECa10 cell lines were affected to different degrees in the secretion of proangiogenic Kapusta-Duch, Teresa Leszczynska ´ cytokines: reducing VEGF and enhancing bFGF secretion. (deepdyve.com)
The regeneration potential of these cells is mainly based on their direct contribution to neovascularization and on their secretion of various paracrine factors activating pro-angiogenic mechanisms [ 13 - 16 ]. (biomedcentral.com)
The results demonstrated that trolox can resist aging, promote proliferation, and enhance PGE-2 secretion of MSCs without affecting their surface marker expression. (techscience.com)
BMSCs interact with uterus parenchymal cells by direct contact or indirect secretion of growth factors to promote functional recovery. (biologists.com)
The PES membrane had better mechanical properties (e.g. ripping), greater chondrocytes proliferation, and thus a better secretion of proteins which build up the cartilage structure. (pan.pl)

The objective of this study is to explore the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles of monocyte-derived dendritic cells (DCs) obtained from peripheral blood mononuclear cells (PBMCs). (frontiersin.org)

retinal pigment epithelial cell line). (deepdyve.com)
The corneal epithelium is composed of stratified squamous epithelial cells on the outer surface of the eye, which acts as a protective barrier and is critical for clear and stable vision. (wjgnet.com)
The severity of the phenotypes correlates with the expression levels of the hEGFR KI allele, which is not efficiently expressed in epithelial and bone cells, but is expressed at similar and even higher levels as the endogenous Egfr in brain and heart. (silverchair.com)
The nanotube bundles are similar to the potential of nanotubes to induce genetic damage size of microtubules that form the mitotic spindle in normal lung cells, cultured primary and immor- and may be incorporated into the mitotic spindle talized human airway epithelial cells were apparatus. (cdc.gov)

Laser scanning cytometry (LSC) generates quantitative information on immune receptor expression from cells cytocentrifuged onto a microscope slide. (ersjournals.com)
In contrast, the quantitative assessment of the immune receptor expression of human adult AM is less problematic, since large volumes of saline are instilled during BAL and the numbers of AM recovered and morphology are suitable for FC analysis 5 . (ersjournals.com)
Using FC, fluorescent antibodies have attached to immune receptors on cells which are then identified as AM by their characteristic autofluorescence, light scatter properties ( e.g. forward scatter) or a combination of both 6 . (ersjournals.com)
To obtain immune receptor data from the cell surface and cytoplasm after nuclear capture, a "contour" is generated at a fixed distance from the nuclear perimeter. (ersjournals.com)
However, the phenotypes of T lymphocytes and their functions in peripheral immune organs and the brain have not been well analyzed in the acute and chronic phases of stroke. (en-journal.org)
Here, we investigated pathological phenotypic alterations in the systemic immune response, especially changes in T lymphocytes, from one day to six months after ischemic stroke in mice. (en-journal.org)
This study provides fundamental information regarding the T lymphocyte alterations from the brain to the peripheral immune organs following stroke. (en-journal.org)
The process of cell death in the brain following a stroke leads to the activation of local immune cells, causing inflammation of the brain tissue. (en-journal.org)
Peripheral immune cells are involved in systemic inflammation. (en-journal.org)
As described below, these diverse situations range from the complications of diabetes and cellular perturbation in amyloidoses to immune and inflammatory responses and tumor cell behavior. (jci.org)
In fish, a variety of specific immune responses such as acute allograft rejection indicate that T cells are involved in these immune activities in a similar manner to those of mammals ( 3 ). (aai.org)
2000 ). The encapsulation of cells has been developed to avoid immune rejection in cell/tissue transplantation. (springer.com)
Human T lymphocytes play an important role in the immune system and have been used in immunotherapy (Juan et al. (springer.com)
Conclusions The gene expression profile of T-cell/histiocyte-rich large B-cell lymphoma, in comparison with that of nodular lymphocyte-predominant Hodgkin's lymphoma, shows features suggestive of a distinct tolerogenic host immune response that may play a key role in the aggressive behavior of this lymphoma, and that may serve as a potential target for future therapy. (haematologica.org)
There are a variety of assays available to test immune function in the dog. (dvm360.com)
Since in classic chemotherapies it is observed a decrease in immune response, methotrexate (MTX) was used only to compare the PnV effects on innate immune cells with a highly immunosuppressive antineoplastic drug. (nature.com)
Analysis of this network shows that it recapitulates known features of the human immune system and can be used uncover novel multi-step immune pathways, examine species-specific differences in immune processes, and predict the response of immune cells to stimuli. (stanford.edu)

Sixty percent of cases express 1 or more T-cell antigens (CD3 + , CD43, or CD45RO). (medscape.com)
The antigens discovered can be used in downstream applications such as single-cell phenotyping with multiparameter flow cytometry and epitope mapping. (antigendiscovery.com)

The number of mature T cells in the spleen and blood decreased and showed reduced interferon-γ production. (en-journal.org)
One cell suspensions of thymocytes, LN cells, spleen cells, PBL, and little intestine intraepithelial lymphocytes (iIEL) had been prepared as defined (26, 27). (tech-strategy.org)

The technique is to produce hybridoma cells that are resultant of fusion of two types of cells that produces monoclonal antibodies. (scienceofhealthy.com)
The significance of using these specific cell types is the production of specific high affinity antibodies in large amounts. (scienceofhealthy.com)
B cells have the ability to produce specific high affinity antibodies but they have finite lifespan that limits the amount of antibody molecules produced by them. (scienceofhealthy.com)
This problem is resolved by myeloma or the cancerous cells that have infinite life span so that they can divide indefinitely, producing high amount of antibodies by the hybridism cell technique. (scienceofhealthy.com)
This cell line has role in the production of humanized monoclonal antibodies. (scienceofhealthy.com)
The result is production of lymohoblastoid cell line that produces monoclonal humanized antibodies and the cells also have infinite life span. (scienceofhealthy.com)
The viral infection activates the B cells for continuous production of monoclonal antibodies. (scienceofhealthy.com)

Design and Methods We collected 33 cases of T-cell/histiocyte-rich large B-cell lymphoma and 56 cases of nodular lymphocyte-predominant Hodgkin's lymphoma and performed microarray gene expression profiling on ten cases of each lymphoma, to obtain a better understanding of the lymphoma host response. (haematologica.org)
Gene expression profiling of lymphomas clearly illustrated that apart from the characteristics of the tumor cells, the microenvironment of the tumor also defines the profile of the lymphoma, and, more importantly, plays a role in predicting the prognosis. (haematologica.org)
The effect of antioxidant trolox on biological characteristics of MSCS, including aging, proliferation, and gene expression, was examined. (techscience.com)

I) and expression levels in CD33+ myeloid cells. (eprf.ca)
decade, Tadalafil used to inhibit the myeloid derived Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, (A Central University), Vidya Vihar, Raebareli road, Lucknow, UP suppressor cells (MDSCs) and restore the T cells in can- 226 025, India cer patients as compared to the normal peoples [4]. (sagepub.com)
In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers of immature blood cells and replacement of normal marrow by malignant cells. (msdmanuals.com)

Dynamic changes in T lymphocytes between the acute and chronic phases may play different roles in pathogenesis and recovery. (en-journal.org)
In recent years, a growing number of studies have shown that stem cells can effectively treat acute liver failure. (springer.com)

To determine the effects of medications on SAA-stimulated human coronary artery endothelial cells (HCAEC). (hindawi.com)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)

MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
2007) (Figures S1H, S2A and Supplemental Experimental Procedure), hiPSC lines were differentiated into hematopoietic progenitor lineages, showing common morphology for the various cell types (Physique S1H). (eprf.ca)
Used together, these scholarly research showed that Compact disc3 and Compact disc3 play an important, yet overlapping partially, role in the introduction of both and T cell CH5424802 biological activity lineages. (tech-strategy.org)

The aggressive T-cell/histiocyte-rich large B-cell lymphoma and the indolent nodular lymphocyte-predominant Hodgkin's lymphoma are both characterized by a paucity of tumor cells embedded in an overwhelming background. (haematologica.org)
The tumor cells of both lymphomas share several characteristics, while the cellular composition of their microenvironment is clearly different. (haematologica.org)
8 , 10 - 12 Recently, genome-wide analysis of isolated tumor cells from NLPHL and THRLBCL revealed further similarities between the tumors cells of the two lymphomas. (haematologica.org)
Apoptotic or necrotic tumor cells discharge DNA fragments into the circulating blood system. (biomedcentral.com)

Under human platelet lysate-supplemented culture conditions, bone marrow- and adipose tissue-derived MSCs exhibited similar fibroblast-like morphology and expression patterns of surface markers. (biomedcentral.com)
Adipose tissue-derived MSCs had greater proliferative potential than bone marrow-derived MSCs, while no significantly difference in colony efficiency were observed between the two types of cells. (biomedcentral.com)
However, bone marrow-derived MSCs possessed higher capacity toward osteogenic and chondrogenic differentiation compared with adipose tissue-derived MSCs, while similar adipogenic differentiation potential wase observed between the two types of cells. (biomedcentral.com)
There were some differences between bone marrow- and adipose tissue-derived MSCs for several secreted proteins, such as cytokine (interferon-γ), growth factors (basic fibroblast growth factor, hepatocyte growth factor, and insulin-like growth factor-1), and chemokine (stem cell-derived factor-1). (biomedcentral.com)
Over the past decade, MSCs which are isolated from the umbilical cord, umbilical cord blood, bone marrow (BM), adipose tissue (AT) and many other adult tissues have been explored as potential therapeutic strategies for a number of diseases [ 2 ], but AT and BM are the most widely used sources of MSC, especially in autologous cell-based therapies due to ease of harvest and potential autologous application [ 3 ]. (biomedcentral.com)
Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
The effects of UC-MSCs on the responses of fibroblast-like synoviocytes (FLSs) and T cells in RA patients were explored. (biomedcentral.com)
On the other hand, UC-MSCs induced hyporesponsiveness of T cells mediated by PGE2, TGF-β1 and NO and UC-MSCs could promote the expansion of CD4 + Foxp3 + regulatory T cells from RA patients. (biomedcentral.com)

Despite its importance, the evolutionary process of the T cell activation system via APCs remains poorly understood because of the lack of an effective model organism in lower vertebrates that could allow one to manipulate leukocytes and to use suitable markers for the identification of APCs. (aai.org)
T cells have been recently identified by using key molecular markers such as CD4 and CD8 ( 4 , 5 ). (aai.org)
2 , 3 , 9 Indeed, the atypical B cells of NLPHL and THRLBCL share many characteristics, including expression of pan-B-cell markers, germinal center B-cell origin and common chromosomal imbalances. (haematologica.org)

RSV signi﫿cantly exhibits anti-in﫿ammatory and anti-angiogenic activities and reg- ulates many cellular signaling pathways associated with cell metabolism, proliferation, differentiation, migration, and invasiveness of normal and cancer cells [7]. (deepdyve.com)
These play a role in cellular stress, metabolism, and autophagy, by interacting with deacetylases activity in the cell. (wikipedia.org)

Results Generation of hiPSC lines and clinical manifestations in NS/JMML-derived hematopoietic cells Using somatic cell reprogramming (Takahashi et al. (eprf.ca)
Open in a separate window Physique 1 Clinical Features of NS/JMML hiPSC-Derived Hematopoietic Cells(A) Hematopoietic growth factor dose-response curves to GM-CSF. (eprf.ca)
13 Despite the similarities of their malignant cells, an important difference between the two lymphomas lies in their clinical presentation and prognosis. (haematologica.org)
Recently, several clinical trials showed an increased left ventricular ejection fraction (LVEF) and an improved regional perfusion following intramyocardial application of cluster of differentiation (CD) 133 + stem cells [ 5 - 9 ]. (biomedcentral.com)
Yet, CD133 + stem cells for clinical use are purified using manual or semi-automatic devices based on the conventional magnetic cell sorting (MACS®) technique. (biomedcentral.com)
The Working Formulation, originally proposed in 1982, classified and grouped lymphomas by morphology and clinical behavior (ie, low, intermediate, or high grade) with 10 subgroups labeled A to J.{Ref 1} In 1994, the Revised European-American Lymphoma (REAL) classification attempted to apply immunophenotypic and genetic features in identifying distinct clinicopathologic NHL entities. (medscape.com)

By quantitative reverse transcriptase polymerase chain reaction we verified that these 20 selected cases were representative of the entire population of T-cell/histiocyte-rich large B-cell and nodular lymphocyte-predominant Hodgkin's lymphomas. (haematologica.org)
1 Initial studies demonstrated that a particular subgroup of T-cell-rich B-cell lymphomas mirror nodular lymphocyte-predominant Hodgkin's lymphoma (NLPHL) and are characterized by a T-cell and histiocyte-rich stroma. (haematologica.org)

Sleeping Elegance is far more susceptible to more than expression inhibition than piggyBac and Tol2, the cargo capacity of Sleeping Attractiveness is constrained, and contrary to Tol2 and piggyBac that Inhibitors,Modulators,Libraries are lively in all mamma lian cell kinds tested, Sleeping Elegance display cell form dependent exercise. (p450inhibitors.com)

K562 cells were the first human immortalised myelogenous leukemia cell line to be established. (wikipedia.org)
Currently, human T cell therapy is of considerable scientific interest. (springer.com)
Here, we propose an innovative technique of single-cell encapsulation of human T cells using polyelectrolytes combined with gold nanorods. (springer.com)
We have demonstrated encapsulation of human Jurkat T cells with poly(sodium 4-styrenesulfonate) (PSS)-coated gold nanorods (PSS-GNRs). (springer.com)
The transplantation of cells or tissues has been applied for treating various human diseases (Bhatia et al. (springer.com)
We analyzed RNA sequencing data from 53 in-house established human melanoma cell lines and 479 melanoma tumors from The Cancer Genome Atlas dataset. (biomedcentral.com)
In order to study the transcriptional regulation of IL32 in these cell lines, we cloned up to 10.5 kb of the 5′ upstream region of the human IL32 gene into a luciferase reporter vector. (biomedcentral.com)
A significant proportion of established human melanoma cell lines express IL32, with its expression being highly correlated with a dedifferentiation genetic signature (high AXL/low MITF). (biomedcentral.com)
However, the role of IL32 in human melanoma cells is less well understood. (biomedcentral.com)
The human melanoma cell lines were established from patient biopsies. (biomedcentral.com)
The myeloma and the B cells of human body fuse to form hybridomas. (scienceofhealthy.com)
Moreover, the human myeloma cells such as Hela cell line poses a problem of its own antibody production in hybridoma culture. (scienceofhealthy.com)
The b lymphocytes of human blood contain receptors for this EBV. (scienceofhealthy.com)
The B lymphocytes of human blood develop lyphoblastoid cell line using EBV virus. (scienceofhealthy.com)
According to the European Medicines Agency (EMA) BM-derived CD133 + stem cells intended for the regeneration of human ischemic heart tissue are classified as an advanced therapy medicinal product (ATMP) [ 17 ]. (biomedcentral.com)
Human GB (NG97, the most responsive line in the previous study) cells were inoculated (s.c.) on the back of RAG −/− mice. (nature.com)
Staining of human peripheral blood lymphocytes with Mouse anti Human CD44:FITC ( MCA89F ). (bio-rad-antibodies.com)
Human cultured fibroblasts of three different donors and three different short-term human lymphocyte cultures were exposed to 1,950 MHz UMTS below the specific absorption rate (SAR) safety limit of 2 W/kg. (arasysperfector.com)
This paper shows cartilage characteristics within its most frequent problems and treatment strategies, including a promising method that combines scaffolds and human cells. (pan.pl)
Effects of sinusoidal magnetic field observed on cell proliferation, ion concentration, and osmolarity in two human cancer cell lines med. (emf-portal.org)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
For several of the human tu- and various assays demonstrated an sues. (who.int)

Myeloproliferative neoplasms (MPNs) BCR-ABL negative are clonal, stem cell diseases. (biomedcentral.com)

Non IL32-expressing differentiated melanoma cell lines exposed to TNFα or IFNγ can be induced to express the three predominant isoforms (α, β, γ) of IL32. (biomedcentral.com)
Melanoma cell lines were treated with 1000 IU/mL TNFα and 100 IU/mL IFNγ (PeproTech US, Rocky Hill, NJ) for the indicated time points. (biomedcentral.com)

Nontoxicity of the developed scaffolds toward T lymphocyte cells was proved. (pan.pl)

Fig.11 and ?and44 and em B /em , T cells were absent in the thymus and periphery of Compact disc3? (tech-strategy.org)
Moreover, small numbers of TCR-+ T cells were detected in the periphery of CD3? (tech-strategy.org)

This results in differing cytotoxic responses that are influenced by the nature of target cells, assay types and concentrations of ATZ. (frontiersin.org)

These functions are necessary in cells in order to divide and regenerate tissues, and play a maintenance role in homeostatic functions. (wikipedia.org)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
While addition of either soybean oil (SO) or fish oil (FO) to PN partially reverses these defects, the effects of the oil composition (FO/SO ratio) on morphology and gut-associated lymphoid tissues (GALT) have yet to be elucidated. (bvsalud.org)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

The transfected cells had been subjected to a chromosomal transposition assay to deter mine their transposition action. (p450inhibitors.com)
Nevertheless, every one of these studies have been based on information sets obtained by retrieving chromosomal targeting sequences from a mixed population of transposon targeted cells or using a PCR based method. (p450inhibitors.com)

The Leukemia-Lymphoma Cell Line Factsbook. (wikipedia.org)
This ulcerated nodule of localized primary cutaneous CD30+ anaplastic large cell lymphoma is relatively nonspecific in appearance. (medscape.com)
Results We observed that the microenvironment in nodular lymphocyte-predominant Hodgkin's lymphoma is molecularly very similar to a lymph node characterized by follicular hyperplasia, while the microenvironment in T-cell/histiocyte-rich large B-cell lymphoma is clearly different. (haematologica.org)
The T-cell/histiocyte-rich large B-cell lymphoma signature is hallmarked by up-regulation of CCL8, interferon-γ, indoleamine 2,3 dioxygenase, VSIG4 and Toll-like receptors. (haematologica.org)
4 In the World Health Organization (WHO) classification of 2001, T-cell/histiocyte-rich large B-cell lymphoma (THRLBCL) is defined by the presence of a limited number of scattered large B cells in a background rich in T cells, with or without histiocytes. (haematologica.org)
6 However, using a stricter definition requiring the presence of a prominent histiocytic component as a major feature and almost complete absence of small B lymphocytes, the unique character of this lymphoma has been demonstrated. (haematologica.org)

Increased numbers of CD4 - CD8 - CD3 + double-negative T cells were observed in the mouse brain during the early stages of stroke, whereas interleukin (IL)-10 + Foxp3 + regulatory T lymphocytes increased from two weeks during the chronic phase. (en-journal.org)
The recovery of T lymphocyte numbers and increases in IL-10 + Foxp3 + regulatory T lymphocytes may be important for long-term neurological outcomes. (en-journal.org)

Rheumatoid arthritis (RA) is a T-cell-mediated systemic autoimmune disease, characterized by synovium inflammation and articular destruction. (biomedcentral.com)
The ability of FLSs to stimulate both inflammation and tissue damage suggests that this cell type may be another critical target for the treatment of inflammatory arthritis [ 11 ]. (biomedcentral.com)

Cell migration and invasion was monitored by transwell chambers assay. (ijpsonline.com)
Cell migration as well as cell invasive ability of MCF-7 cells was reduced to minimum by the application of piperine in dose dependent-manner. (ijpsonline.com)
Proteomic-based GO analysis demonstrated that biological processes such as cell deformation, proliferation, migration and wound healing occurred in the liver during the early infection. (biomedcentral.com)

The first type is specific antibody generating B lymphocyte cells and others the immortal myeloma or cancerous cell. (scienceofhealthy.com)

For hereditary disorders associated with cancer such as NS/JMML, AM 114 hiPSCs derived from non-cancerous cells permit investigation of the role of AM 114 the inherited mutations mutations. (eprf.ca)
To monitor morphology of cancerous breast cells, phase contrast microscopy was performed after piperine exposure. (ijpsonline.com)

To date, where AM numbers are limited or if AM morphology is required, image analysis (IA) systems such as confocal microscopy have been used 8 . (ersjournals.com)

CD133 + stem cells were purified from patient-derived sternal bone marrow using the recently developed automatic CliniMACS Prodigy® BM-133 System (Prodigy). (biomedcentral.com)

The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found 16 significant regulated pathways in imDCs and 10 significant regulated pathways in mDCs, including the phagosome, cell adhesion signaling pathway, and inflammatory mediator regulation of TRP channels pathway. (frontiersin.org)

When CB is administered along with the allergen to mice, there is a significantly augmented response in the draining popliteal lymph node including increases in weight, cell numbers, cell proliferation, and local lymph node response duration [ 867 ]. (nanomedicine.com)

K562 cells are part of the NCI-60 cancer cell line panel used by the National Cancer Institute. (wikipedia.org)
The problem with K562 cells, and many other cancer cell types, is an overabundance of Aurora kinases. (wikipedia.org)
Inhibiting these is an important regulation mechanism of cancer, because it prevents cells from progressing into mitosis. (wikipedia.org)
The ability to induce these changes in K562 cell cycle and cell cycle regulation provides targets for cancer drugs. (wikipedia.org)
Clonogenic assay was implemented for checking the effect of piperine on clonogenic potency of breast cancer cells. (ijpsonline.com)
Further, application of piperine on cancer cell colonies remarkably reduced the number of cell colonies to minimum. (ijpsonline.com)
Flow cytometric analysis evidenced that piperine arrested the cell cycle at growth 2/mitosis phase, hence suppressed the breast cancer progression. (ijpsonline.com)
In conclusion, the current investigation regarding anticancer effects of piperine against breast cancer revealed remarkable suppression of cancer cells. (ijpsonline.com)
Tadalafil is a known phosphodiesterase-5 Distinct metastasis and resistance development by self- (PDE-5) inhibitor, which have anti-cancer effects in vari- renewing nascent cancer cells in response with the ous types of cancer [2]. (sagepub.com)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

The optimal dose of AH in the basal medium (FD) and medium containing serum (FDS) for keratocytes proliferation was identified using MTT assay. (blogspot.com)

280 independent research projects studying β-cell physiology, β-cell differentiation, immunology, T1D biomarkers, technology development, T1D pathology, and diabetes etiology ( https://www.jdrfnpod.org/publications/current-npod-projects/ , accessed October 21, 2021). (nature.com)

Treatment includes induction chemotherapy to achieve remission and postremission chemotherapy (with or without stem cell transplantation) to avoid relapse. (msdmanuals.com)
Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)

These data suggest a key role for HSP70 in proliferation and survival of the erythroid lineage in PV, and may represent a potential therapeutic target in MPN, especially in PV. (biomedcentral.com)
The WHO modification of the REAL classification of NHL is based on morphology and cell lineage. (medscape.com)

Fugu B7-H1/DC inhibited T cell proliferation concomitant with increasing levels of both IL-10 and IFN-γ expression, whereas both B7-H3 and B7-H4 promoted T cell growth following IL-2 induction and the suppression of IL-10. (aai.org)

Within the B-cell and T-cell categories, two subdivisions are recognized: precursor neoplasms, which correspond to the earliest stages of differentiation, and more mature differentiated neoplasms. (medscape.com)

These APCs capture and process Ags and then present the processed antigenic peptides upon MHC molecules to the T cells. (aai.org)

Subsequently, the APCs activate T cells with costimulatory molecules upon the APCs ( 1 ). (aai.org)

These data provide promising results, demonstrating the potential use of encapsulated PSS-GNR/PAH@Jurkat to provide a more inert T cell population for immunotherapy application and other biomedical applications. (springer.com)
these results confirm that this cell is a target of PnV components. (nature.com)
Results: On the grounds of hemodynamic recordings and morphology, n-methyl n-nitrosourea treated group showed distorted changes along with distorted morphological parameters. (sagepub.com)
Correspondingly, transcriptomic results showed significant enrichment of cell cycle pathway on day 3 and 7. (biomedcentral.com)
These results show that LMP1 , mour viruses classified by IARC in EBV-specific T-cell response. (who.int)

Anatomy44

Organisms17

Diseases10

Chemicals and Drugs88

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Phenomena and Processes58

Disciplines and Occupations1

Information Science3

Vitro17

Functional assays1

Apoptosis22

Mesenchymal stem8

Macrophages11

Viability4

Immunophenotype3

Infiltration3

Monocytes3

20161

Cellular morphology1

Morphological changes2

Protein10

Antigen8

Progenitor cells4

Secretion5

Dendritic cells1

Epithelial4

Immune17

Antigens2

Spleen2

Antibodies7

Gene expression3

Myeloid3

Acute2

Endothelial cells2

Lineages3

Tumor cells4

MSCs8

Markers3

Metabolism2

Clinical6

Lymphomas2

Inhibitors1

Human22

Clonal1

Melanoma2

Scaffolds1

Periphery2

Cytotoxic1

Tissues4

Chromosomal2

Lymphoma6

Foxp32

Inflammation2

Migration3

Antibody1

Cancerous2

Microscopy1

Bone1

Pathways1

Lymph1

Cancer11

Serum1

Immunology1

Stem cell2

Lineage2

Induction1

Neoplasms1

Antigenic1

Activate T cells1

Results5