Morphology clone cells. Medical search. Frequent questions

Functional annotation of the high confidence genes in QRl.hwwg-1AS and QRl.hwwg-5AL revealed 53 putative candidate genes encoding proteins for stress response and cell morphology. (usda.gov)
Among these genes, the rice orthologs of TraesCS1A03G0023500 and TraesCS1A03G0027900 in the QRl.hwwg-1AS interval and TraesCS5A03G0631600, TraesCS5A03G0631700 and TraesCS5A03G0657300 in the QRl.hwwg-5AL interval have been cloned previously and demonstrated to regulate RL through modulating cell morphology in rice. (usda.gov)
Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. (sigmaaldrich.com)
Genes were cloned by PCR and analysed phylogenetically. (washington.edu)
The cloned genes encode R2R3 MYB proteins closely related to Antirrhinum AmMYBML2 and Petunia hybrida PhMYB1. (washington.edu)
Compared to the large airway cell line BCi-NS1.1, differentiated of hSABCi-NS1.1 cells on ALI were enriched with small airway epithelial genes, including surfactant protein genes, LTF and small airway development relevant transcription factors NKX2-1, GATA6, SOX9, HOPX, ID2 and ETV5. (biomedcentral.com)
Here, we attempted to create genetically engineered hepatoma cells with enhanced liver functions by overexpression of liver-enriched transcription factors (LETFs), which are associated with the transcription of liver-specific genes and hepatic differentiation. (elsevierpure.com)
Mouse hepatoma Hepa1-6 cells were transduced with retroviral vectors, in which inducible expression cassettes for the LETF genes were introduced. (elsevierpure.com)
Upon overexpression of the LETF genes, cell proliferation ceased and the cells exhibited an epithelial morphology, indicating hepatic maturation of hepatoma cells. (elsevierpure.com)
Mesodermal lineage induction of PLA cells and clones resulted in the expression of multiple lineage-specific genes and proteins. (livingroom.health)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

In vitro bioassay for dioxinlike activity based on alterations in epithelial cell proliferation and morphology. (cdc.gov)
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been shown to induce changes in morphology and proliferation characteristics of a nonkeratinizing derivative (XBF) of a keratinizing epithelial cell line (XB), cloned from a mouse teratoma, when cocultured with irradiated feeder cells. (cdc.gov)
IM combined with IR can synergistically inhibit the proliferation, apoptosis, clonogenesis, invasion, migration and cell cycle of GIST cells. (researchsquare.com)
The molecular mechanism underlying its development is complex, but its carcinogenesis has been proposed to occur with cell proliferation and resistance to apoptosis, driven by the signaling activity of abundant EGFR and receptor tyrosine-protein kinase erbB-2. (bvsalud.org)
These results supported the proposed cytotoxic effects of CUDC-101, in addition to its inhibitory effects on cell division and proliferation in EGFR-overexpressing bladder cancer cells. (bvsalud.org)
CCK-8, transwell invasion, wound healing and clonogenic survival assays were used to examine the proliferation, invasion, migration and radiosensitivity of ESCC cells in vitro. (bvsalud.org)
Mechanistically, EBF2 uniformly activates Pparγ-expression in pancreatic cancer cells and limits cell proliferation, whereas oncogenic drivers are expressed differentially in the progenitor cell subsets. (amegroups.org)
The complex PDAC pathobiology is established predominantly through KRAS mutations and the associated cellular signaling that contributes to cell proliferation and dedifferentiation ( 5 , 6 ). (amegroups.org)
An accompanying proliferation of erythroid cells and megakaryocytes usually is present. (medscape.com)
Using a multi-task learning approach to identify the biological tasks of each archetype, we identify metabolism and proliferation, cellular defense response, and DNA repair signaling as consistent cell states found across patients. (edu.au)
Our analysis demonstrates a shift in favor of the metabolism and proliferation archetype versus cellular defense response archetype in cancer cells that received multiple lines of treatment. (edu.au)
Загальні відомості про плазмоклітинні захворювання Plasma cell disorders are a diverse group of disorders of unknown etiology characterized by Disproportionate proliferation of a single clone of B cells Presence of a structurally and electrophoretically. (msdmanuals.com)
Mesenchymal cells were isolated from periodontal ligament of impacted third molars (n=2) and cultured on titanium disks (polished and nitrided) and on a plastic surface as a positive control of cell proliferation. (bvsalud.org)
Cell proliferation was analyzed and growth curves were constructed for the different groups by determining the number of cells adhered to the different surfaces at 24, 48 and 72 h after plating. (bvsalud.org)
We concluded that plasma nitriding produced surfaces that permitted the proliferation of human periodontal ligament mesenchymal cells. (bvsalud.org)
Various biological events associated with bone healing on implant surfaces can be investigated separately using appropriate cell cultures, such as cell adhesion, proliferation and differentiation, as well as the production and mineralization of extracellular matrix. (bvsalud.org)
In this respect, this study evaluated the proliferation of mesenchymal cells derived from the periodontal ligament of human third molars on polished and plasma-nitrided titanium surfaces. (bvsalud.org)
ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

Serotypes are identified by reaction with serotype-specific monoclonal (clones from a single cell) antibodies or by biological characteristics such as host range, pathogenicity (severity of disease), growth rate, and plaque morphology (the physical appearance of laboratory grown viral cultures). (poultryhub.org)
The BR-XJ160 virus raised in BHK-21 cells was indistinguishable from the XJ-160 virus in its biological properties, including its plaque morphology, growth kinetics and suckling mouse neurovirulence. (biomedcentral.com)
Strain L9 produced plaques in the HRT-18 clones, but the ease of plaque formation and plaque morphology was host cell dependent. (lsu.edu)
Host cell-dependent plaque formation was demonstrated by wild-type BCV strains, and plaque morphology was strain dependent. (lsu.edu)

Here we report the generation of clonal NP cell lines from nondegenerate human IVD tissue and present a detailed characterization of NP cellular subpopulations. (biomedcentral.com)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)

All cells grew at 33 degrees C, but did not at nonpermissive temperature (39 degrees C). High expression level of large T-antigen in the nuclei was observed at 33 degrees C, whereas the expression level was gradually decreased in a time-dependent manner at 39 degrees C. These results suggest that the temperature-sensitive growth characteristics arise as a result of a function of the tsSV40 large T-antigen. (wikigenes.org)
Detailed understanding of the ontogenetic, cellular and molecular characteristics of functional intervertebral disc (IVD) cell populations is pivotal to the successful development of cell replacement therapies and IVD regeneration. (biomedcentral.com)
Successful development of cell replacement therapies and IVD regeneration is crucially dependent on an in-depth understanding of cellular and molecular characteristics of the functional IVD. (biomedcentral.com)
Deletion of HP1β, but not HP1α, in ESCs provokes a loss of the morphological and proliferative characteristics of embryonic pluripotent cells, reduces expression of pluripotency factors and causes aberrant differentiation. (biomedcentral.com)
Finally, PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression. (livingroom.health)
Surface treatment permits the modification of the dental implants characteristics, such as chemical composition, morphology, topography and rugosity 5 . (bvsalud.org)

NS0 cells are a model cell line derived from the nonsecreting murine myeloma used in biomedical research and commercially in the production of therapeutic proteins. (wikipedia.org)
One of these components, the Mdm1p protein, is a cytoskeletal element that exhibits structural similarities to the intermediate filament proteins of animal cells ( McConnell and Yaffe, 1992 , 1993 ). (rupress.org)
These proteins control the dynamics of the actin cytoskeleton and thus represent key regulatory molecules that are active during cell migration ( 12 ). (spandidos-publications.com)
These porous microstructures allow cell infiltration into the matrix and have fewer heterogeneous cellular proteins, thereby preventing complications, such as inflammatory reactions, fibrosis, calcification, and graft shrinkage. (biomedcentral.com)
In infected cells, the virion structural proteins are translated from a subgenomic mRNA (26S RNA) and produced by transcription of genome-length complementary (minus) strand from a highly active subgenomic promoter [ 5 ]. (biomedcentral.com)
In addition to mesodermal capacity, PLA cells and clones differentiated into putative neurogenic cells, exhibiting a neuronal-like morphology and expressing several proteins consistent with the neuronal phenotype. (livingroom.health)

Our cell lines yield novel insights into NP biology and provide promising new tools for studies of IVD development, cell function and disease. (biomedcentral.com)
To facilitate the study of the biology of the human SAE in health and disease, we immortalized and characterized a normal human SAE basal cell line. (biomedcentral.com)
The immortalized hSABCi-NS1.1 cell line has diverse differentiation capacities and retains SAE features, which will be useful for understanding the biology of SAE, the pathogenesis of SAE-related diseases, and testing new pharmacologic agents. (biomedcentral.com)
In this context, if it is possible to immortalize normal human SAE BC that retain the capacity to differentiate to ciliated, secretory, and other differentiated cell types in vitro on air-liquid interface (ALI) culture, it would be very useful to the investigation of SAE biology in health and disease, and the assessment of pharmacologic agents targeted to modify dysregulated BC biology relevant to the pathogenesis of human lung disease. (biomedcentral.com)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
In addition, cluster of differentiation 24 (CD24)-negative NP responder clones formed spheroid structures in various culture systems, suggesting the preservation of a more immature phenotype compared to CD24-positive nonresponder clones. (biomedcentral.com)
Differential cell surface marker expression and divergent responses to differentiation conditions suggest that the NP subtypes may correspond to distinct maturation stages and represent distinct NP cell subpopulations. (biomedcentral.com)
Additionally, lingering expression or re-expression of viral transgenes in iPSC-derived cells can interfere with their differentiation potential. (addgene.org)
The basal cells (BC) are the stem/progenitor cells of the SAE, responsible for the differentiation into intermediate cells and ciliated, club and mucous cells. (biomedcentral.com)
The resulting cell line (hSABCi-NS1.1) was characterized by RNAseq, TaqMan PCR, protein immunofluorescence, differentiation capacity on an air-liquid interface (ALI) culture, transepithelial electrical resistance (TEER), airway region-associated features and response to genetic modification with SPDEF. (biomedcentral.com)
Here we show that cell differentiation suppresses tumor formation. (amegroups.org)
Cells were transfected with the early B-cell factor-2 (EBF2) to activate progenitor cell differentiation and gauged the tumorigenic potentials of the derived cell subsets. (amegroups.org)
Here we show that the brown adipose differentiation gene, EBF2, activates differentiation-associated signals in the cancer progenitor cells in culture and CSC transplantation models. (amegroups.org)
This study reveals a previously unrecognized function of EBF2 in regulating PDAC progenitor cell differentiation and tumor suppression. (amegroups.org)
Thus, exogenous AhR induced catecholaminergic differentiation in N2a-Rα cells. (biomedcentral.com)
The excessive activation of AhR resulted in neural differentiation of Neuro2a cells. (biomedcentral.com)
Activated AhR may disrupt the strictly regulated brain formation with irregular differentiation occurring rather than cell death. (biomedcentral.com)
However, in differentiated cells, loss of HP1β has the opposite effect, perturbing maintenance of the differentiation state and facilitating reprogramming to an induced pluripotent state. (biomedcentral.com)
We demonstrate an unexpected duality in the role of HP1β: it is essential in ESCs for maintaining pluripotency, while it is required for proper differentiation in differentiated cells. (biomedcentral.com)
This unique duality makes them an attractive system for potential regenerative medicine and cell therapies, but also for differentiation studies in vitro and for modeling diseases. (biomedcentral.com)

Targeted clone R-Olig2 (like the other clones) retained pluripotency, typical hESC morphology, and a normal parental karyotype 46,XY. (ca.gov)
Embryonic stem cells (ESCs), derived from the blastocyst-stage embryo, are capable of generating all cell types of the mammalian body (pluripotency) and of maintaining the capacity for indefinite self-renewal without compromising their genomic integrity. (biomedcentral.com)
cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)

Gastric mucosal cells and epithelial tissues isolated from the stomach of the transgenic rats were cultured at permissive temperature (33 degrees C), and proliferative cells were cloned by colony formation. (wikigenes.org)
To evaluate the proliferative capacity of mesenchymal cells derived from human periodontal ligament on polished and plasma-treated titanium surfaces. (bvsalud.org)
Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
We developed a novel 4-color mIHC assay based on tyramide signal amplification that allowed us to reliably interrogate immunologic checkpoints, including programmed death-ligand 1 (PD-L1), cytotoxic T cells (CD8 + T) and regulatory T cells (Foxp3), in formalin-fixed, paraffin-embedded tissues of various human gastric diseases. (biomedcentral.com)
We found that PD-L1 was expressed in gastric ulcer and in tumor cells (TCs), as well as in tumor-infiltrating immune cells (TIICs), but not in normal gastric mucosa or other gastric intraepithelial neoplastic tissues. (biomedcentral.com)
Furthermore, we found no significant reduction in CD8 + T cells, whereas the ratio of CD8 + T:Foxp3 cells and CD8 + T:PD-L1 cells was suppressed in tumor tissues and elevated in adjacent normal tissues. (biomedcentral.com)
In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)
Tissues with complex three-dimensional morphology, significant depth, or rapid movement are challenging to selectively target by optical techniques. (cdc.gov)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

Both exhibited cuboidal morphology and junctional complexes, but did not contain lamellar bodies or express surfactant protein A, B or C mRNAs. (ersjournals.com)
Furthermore, E1A DNA and protein persisted in epithelial cells in the lungs of these animals 9 . (ersjournals.com)
Moreover, a D614G mutation in the spike protein did not appear to affect SARS-CoV-2 cell tropism. (cdc.gov)
Mdm12p is the third mitochondrial outer membrane protein required for normal mitochondrial morphology and distribution to be identified in S. cerevisiae and the first such mitochondrial component that is conserved between two different species. (rupress.org)
Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
When cultured on ALI, hSABCi-NS1.1 cells consistently formed tight junctions and differentiated into ciliated, club (SCGB1A1 + ), mucous (MUC5AC + , MUC5B + ), neuroendocrine (CHGA + ), ionocyte (FOXI1 + ) and surfactant protein positive cells (SFTPA + , SFTPB + , SFTPD + ), observations confirmed by RNAseq and TaqMan PCR. (biomedcentral.com)
One such immune checkpoint is programmed cell death protein 1 (PD-1). (biomedcentral.com)
Host cell-dependent differences in the protein profile of L9 were detected. (lsu.edu)
Cleavage of the 185 Kd protein, an increase in the 100 Kd band, and conversion of the 31-34 Kd cluster to a 34 Kd band occurred when BCV-L9 was propagated in D2BFS cells with trypsin. (lsu.edu)

Despite improvements in the CRISPR molecular toolbox, identifying and purifying properly edited clones remains slow, laborious, and low-yield. (nature.com)
How cells compete is poorly understood, but it is generally accepted that molecular exchange between cells signals elimination of unfit cells. (nature.com)
Here we show that scrib KD cells are out-competed by wild-type cells through mechanical insults rather than molecular exchange. (nature.com)
Although studies have identified unique transcription factors in the selection of normal pancreatic lineages, the cells of origin and molecular mechanisms arbitrating PDAC growth are poorly defined. (amegroups.org)
To confirm whether adipose tissue contains stem cells, the PLA population and multiple clonal isolates were analyzed using several molecular and biochemical approaches. (livingroom.health)
During the early 1990s, breakthroughs in cloning and sequencing of Norwalk virus and Southampton virus ( 15--18 ) led to the development of sensitive molecular assays (e.g., reverse transcription-polymerase chain reaction [RT-PCR]), nucleotide hybridization probes, and enzyme-linked immunosorbent assays (ELISA) that used baculovirus-expressed viral antigens ( 19--33 ). (cdc.gov)

Cancer stem cells (CSCs) and poorly differentiated progenitor cells in pancreatic ductal adenocarcinoma (PDAC) exacerbate hierarchical tissue organization contributing to cell hyperproliferation and therapy resistance. (amegroups.org)
The attached embryos robustly generated outgrowths comprising Oct4-positive cells indicating the presence of Sera progenitor cells (Supplementary info Data S1 Number 1C). (researchhunt.com)

Here, we establish a method to enable clonal isolation, selection, and expansion of properly edited cells, using OptoElectroPositioning technology for single-cell manipulation on a nanofluidic device. (nature.com)
Each colony is split and sequentially exported for on-target sequencing and further off-chip clonal expansion of the validated clones. (nature.com)
In this study, we aimed to investigate whether phenotypically distinct clonal cell lines representing different subpopulations in the human NP could be generated using immortalization strategies. (biomedcentral.com)
Acute lymphocytic leukemia (ALL) is a malignant clonal disorder of the bone marrow lymphopoietic precursor cells. (medscape.com)
Acute lymphoblastic leukemia (ALL) is a malignant (clonal) disease of the bone marrow in which early lymphoid precursors proliferate and replace the normal hematopoietic cells of the marrow. (medscape.com)

Gene amplification is typically performed using GS-transfected NS0 cells to select for producing cell lines. (wikipedia.org)
Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)
Cell engineering through gene editing is fundamentally a two-step bioprocess. (nature.com)
We show that MDCK cells silenced for the polarity gene scribble ( scrib KD ) are hypersensitive to compaction, that interaction with wild-type cells causes their compaction and that crowding is sufficient for scrib KD cell elimination. (nature.com)
It has recently been reported that Madin-Darby canine kidney (MDCK) epithelial cells silenced for the polarity gene scribble ( scrib KD cells) are eliminated in the presence of wild-type MDCK cells 23 , while they are viable on their own 23 . (nature.com)
Saccharomyces cerevisiae cells lacking the MDM12 gene product display temperature-sensitive growth and possess abnormally large, round mitochondria that are defective for inheritance by daughter buds. (rupress.org)
Retrovirus has a cloning capacity of ~8 kb, so either a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming factors. (addgene.org)
In this study, a gene encoding for an rpoS -like sigma factor, rpoX , has been cloned and characterized. (hindawi.com)
Test chemical did not induce mutation in mammalian cell line in the presence and absence of metabolic activation and hence it is not likely to classify as a gene mutant in vitro. (europa.eu)
11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)

Differences were evident in growth kinetics and plating efficiency of each clone. (lsu.edu)

Moreover, even when high-purity single-cell sorting can be achieved, viability after sorting is often low to moderate, especially for cell types that are particularly sensitive to hydrodynamic stress or low-density culture conditions (e.g., primary cells or pluripotent stem cell lines). (nature.com)
Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
The field of induced pluripotent stem cells (iPSCs) has been around for 10 years. (addgene.org)
Editor Embryonic stem cells (ESCs) are pluripotent cells established from early stage embryos that maintain the capability to differentiate into three-germ level cells. (researchhunt.com)
Pluripotent embryonic stem cells (ESCs) have the unique ability to differentiate into every cell type and to self-renew. (biomedcentral.com)
In pluripotent and differentiated cells HP1β is differentially localized and differentially associated with chromatin. (biomedcentral.com)
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? (hpscreg.eu)
Does consent explicitly allow the derivation of pluripotent stem cells? (hpscreg.eu)
Does consent expressly prevent the derivation of pluripotent stem cells? (hpscreg.eu)

We find that scrib KD cells are hypersensitive to compaction and that this is due to elevation of baseline p53 levels, which is both necessary and sufficient to induce hypersensitivity to crowding and confer a mechanical loser status. (nature.com)
The test chemical did not induce chromosome aberrations in the mammalian cell line in the presence and absence of S9 metabolic activation system and hence it is not mutagenic in the chromosome aberration study performed. (europa.eu)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)

Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)
Reports from several laboratories suggest that a phenomenon termed epithelial-mesenchymal transformation, in which epithelial cells transform into fibroblasts capable of producing connective tissue matrix, is an important feature of tissue remodelling 10 , 11 . (ersjournals.com)
The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)
Here the authors use innovative approaches to combine spatial profiling with single cell transcriptomics to define tumor-proximal populations of fibroblasts that predict clinical outcome. (elifesciences.org)
This may be due, in part, to a type of cell called fibroblasts. (elifesciences.org)
Not only do these wrap pancreatic tumours in a dense, protective layer, they also foster complex relationships with the cancerous cells: some fibroblasts may fuel tumour growth, while other may help to contain its spread. (elifesciences.org)
Being able to specifically eliminate pro-cancer fibroblasts requires a better understanding of the factors that shape the role of these cells, and how to identify them. (elifesciences.org)
RhoA has been implicated in the formation of stress fibers and cell adhesion in fibroblasts. (spandidos-publications.com)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
Currently, the efficiency for nuclear transfer is between 0-10%, i.e., 0-10 live births after transfer of 100 cloned embryos. (biomedcentral.com)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

abstract = "Hepatoma cells, which are derived from liver carcinoma, are able to proliferate infinitely under culture conditions. (elsevierpure.com)
Abstract Starting from mature vegetable compost, four bac- the utilization of these bacteria as cell factories for upgrading terial strains were selected using a lignin-rich medium. (lu.se)

Although we repeated all results under the same or slightly different conditions, some of our results were further confirmed using multiple assay methods on divergent SARS-CoV-2 strains and in cell lines from different sources. (cdc.gov)
In the beginning preimplantation blastocysts (embryonic day time 4.5) from an outbred strain of CD:VRL1 rats (also called Sprague-Dawley (SD)) and inbred strains of Fischer 344 and Brown-Norway (BN) rats were individually seeded onto mitomycin C-treated mouse embryonic fibroblast cells in N2B27 medium supplemented with 2i-LIF (Supplementary info Data S1 Number 1A and ?and1B).1B). (researchhunt.com)

A failure of maturation of the neoplastic cell clone exists. (medscape.com)
In leukemias, a clone of malignant cells may arise at any stage of maturation, that is, in the lymphoid, myeloid, or pluripotential stage. (medscape.com)

16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)

This assay system using XBF cells cocultured with irradiated 3T3 fibroblast feeder cells was examined as a possible in vitro screening assay for dioxinlike activity by testing benzene extracts of soot from a fire involving a PCB-containing transformer. (cdc.gov)
To investigate the mechanisms of scrib KD -mediated cell competition, we first asked whether it is mediated by soluble factors, as in other cases of in vitro cell competition 6 , 24 . (nature.com)
To address this issue, the expression levels of Snail, RhoA and fibronectin, as well as MMP‑2, were reduced in the breast tumor cell lines MDA‑MB‑231 and MDA‑MB‑435S, and their biological responses were studied in vitro and in vivo. (spandidos-publications.com)
Cells are collected from donor (a) and cultured in vitro (b). (biomedcentral.com)
Recombinant plasmid pBR-XJ160 is an infectious full-length cDNA clone of XJ-160 virus, from which rescued virus BR-XJ160 can be obtained by transcription in vitro and transfection. (biomedcentral.com)
In vitro models have been proposed in an attempt to better understand the early stages of osseointegration and how cells interact with surfaces modified by different methods. (bvsalud.org)

The generation of the embryonic CNS is a lineage-based process in which neural progenitors, called neuroblasts (NBs), give rise to largely invariant lineages of neural/glial cells. (biologists.com)
Cell lineage analysis techniques have been used to analyse most of the embryonic NB lineages at the histological level. (biologists.com)
A targeted neuroglial reporter line generated by homologous recombination in human embryonic stem cells. (ca.gov)
In this study, we targeted Olig2, a basic helix-loop-helix transcription factor that plays an important role in motoneuron and oligodendrocyte development, in human embryonic stem cell (hESC) line BG01 by homologous recombination. (ca.gov)
One important insight came from the discovery that the increased motility and invasiveness of cancer cells is reminiscent of the epithelial-mesenchymal transition (EMT) that occurs during embryonic development ( 2 ). (spandidos-publications.com)
Amount 1 (A) System from the derivation of embryonic stem cells. (researchhunt.com)

Selection of edited cell clones currently relies on limiting dilution or fluorescence-activated cell sorting (FACS)-based single-cell sorting to isolate single cells. (nature.com)
Most importantly, GFP expression recapitulated endogenous Olig2 expression when R-Olig2 was induced by sonic hedgehog and retinoic acid, and GFP-positive cells could be purified by fluorescence-activated cell sorting. (ca.gov)

Human rectal tumor-18 (HRT-18) cell clones 3F3, 3E3, D2, and 4B3 exhibited differences in cellular morphology in Giemsa-stained cultures and developing monolayers. (lsu.edu)

Random integration also creates heterogeneous iPSC cell lines, which can complicate comparisons made between lines. (addgene.org)
Finally, retroviruses are prone to insertional mutagenesis and random integration creates variation between iPSC clones. (addgene.org)
Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)

Isolating and propagating viruses from clinical specimens in cell cultures or embryonated chicken eggs is widely used to identify multiple viruses and produce vaccines, mostly under Biosafety Level 2 containment. (cdc.gov)
Growth rate (doubling time) profiles showed that scrib KD cells in pure cultures divide, albeit at a reduced rate, to reach a steadily maintained number ( Supplementary Fig. 1d ), whereas under competing conditions, their numbers collapse following initial growth ( Fig. 1a ). (nature.com)
Interestingly, we found that the growth rate of scrib KD cells is not affected by conditioned medium from competing cultures ( Fig. 1b and Supplementary Fig. 1c ). (nature.com)
Early passage NP monolayer cell cultures were initially characterized using a recently established NP marker set. (biomedcentral.com)
Cell cultures have been extensively used in implantology to evaluate the effect of the substrate on the behavior of osteoblasts during osseointegration. (bvsalud.org)
Within this context, cell cultures offer unique insights into the process and phenomenon of osseointegration 1 . (bvsalud.org)

Has the cell line karyotype been analysed? (ebisc.org)

The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA. (sigmaaldrich.com)
Retroviral vectors , such as pMXs or pMSCV, infect dividing cells, and have a higher efficiency of generating iPSCs compared to other delivery methods. (addgene.org)
Lentiviral vectors infect dividing and nondividing cells and have a larger cloning capacity (8-10 kb) than other retroviral vectors. (addgene.org)

Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
Hs 578T are epithelial cells isolated from breast tissue derived from a 74-year-old, White, female breast cancer patient. (atcc.org)
Marek's Disease (MD) results in enlarged nerves and in tumour formation in nerve, organ, muscle and epithelial (cells that line the internal and external surfaces of the body) tissue. (poultryhub.org)
In this process, epithelial cells acquire fibroblast-like properties such as the functional loss of E-cadherin. (spandidos-publications.com)

Preliminary studies have recently identified a putative stem cell population within the adipose stromal compartment. (livingroom.health)

1. Proteases regulate cells by cleaving proteinase-activated receptors (PARs). (nih.gov)
Thrombin and trypsin cleave PAR-1 and PAR-2 on neurons and astrocytes of the brain to regulate morphology, growth and survival. (nih.gov)
We hypothesized that thrombin and mast cell tryptase, which are generated and released during trauma and inflammation, regulate enteric neurons by cleaving PAR-1 and PAR-2. (nih.gov)

These two cell types differed, however, in their NF-κB and AP-1 binding after lipopolysaccharide stimulation, possibly due to differences in the expression of the subunits that comprise these transcriptional complexes. (ersjournals.com)
When genome editing induces a phenotypic alteration that is detectable by fluorescence (i.e., cell-surface expression of a target that can be non-lethally assessed with fluorescently labeled antibody), FACS provides a method of enriching edited cells 9 , narrowing the number of clones to propagate and analyze. (nature.com)
Furthermore, we show that Notch signalling positively regulates glial cells missing ( gcm ) expression in the context of SPG development. (biologists.com)
Spatial expression by in situ hybridization overlaps areas of conical cells. (washington.edu)
NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
Lentivirus-mediated expression of SPDEF in hSABCi-NS1.1 cells induced secretory cell metaplasia, accompanied with characteristic COPD-associated SAE secretory cell changes, including up-regulation of MSMB, CEACAM5 and down-regulation of LTF. (biomedcentral.com)
We confirmed expression of PAR-1 and PAR-2 in the myenteric plexus by RT-PCR using primers based on sequences of cloned guinea-pig receptors. (nih.gov)
The activation of exogenous AhR in N2a-Rα cells was confirmed using RNAi, with si-AhR suppressing the expression of exogenous AhR. (biomedcentral.com)
Morphologically, N2a-Rα cells bore spontaneous neurites exhibiting axon-like properties with the localization of NF-H. In addition, cdc42 expression was increased in comparison to the control cell line. (biomedcentral.com)
Cell clones with inducible expression of high liver functions were established. (elsevierpure.com)
Bovine Enteropathogenic Coronavirus: The Effect of the Host Cell and Trypsin Modification on the Virus Structure, Cytopathic Expression, and Infectivity. (lsu.edu)
Cytopathic expression (CPE) including cytoplasmic vacuolization and cell fusion occurred in BCV-L9-infected clones 3F3, D2, and 3E3. (lsu.edu)

Progress has been made to enhance the efficiency of HDR-based editing 8 , however, a technology to identify cells with desired mono- or bi-allelic edits is urgently needed to realize the full potential of CRISPR. (nature.com)
Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)
Although the efficiency of nuclear transfer has been dramatically improved from the initial success rate of one live clone born from 277 embryo transfers [ 1 ], none of the aforementioned efforts abolished the common problems associated with nuclear transfer. (biomedcentral.com)

The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and wait for cells to de-differentiate. (addgene.org)
Here, we demonstrated the feasibility of using autologous urine-derived stem cells (USCs) seeded on small intestinal submucosa (SIS) to repair a urethral defect in a rabbit model. (biomedcentral.com)
The last mentioned had been similar to look at NVP-AUY922 to mouse epiblast stem cells (Amount 1B). (researchhunt.com)
Much of the work conducted on adult stem cells has focused on mesenchymal stem cells (MSCs) found within the bone marrow stroma. (livingroom.health)
The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)

We show that C. difficile reversibly differentiates into rough and smooth colony morphologies and that bacteria derived from the isolates display discrete motility behaviors. (nih.gov)
We identified an atypical phase-variable signal transduction system consisting of a histidine kinase and two response regulators, named herein colony morphology regulators RST (CmrRST), which mediates the switch in colony morphology and motility behaviors. (nih.gov)
Microscopic analysis of cell and colony structure indicates that CmrRST promotes the formation of elongated bacteria arranged in bundled chains, which may contribute to bacterial migration on surfaces. (nih.gov)
Trisomy 8+ cells showed a significant positive correlation with apoptotic CD34+ cells and capacity for colony formation. (who.int)

Several therapeutic antibody products are produced using the NS0 cell line including daclizumab and eculizumab. (wikipedia.org)
These will provide the ability to evaluate and compare data from a number of different cell lines which will facilitate greater insight into the cause of the ASDs and will be extremely useful for uncovering new therapeutic and diagnostic targets. (topsan.org)
In the present study, T24 bladder cancer cell lines with EGFR-overexpression were constructed, before the multi-target inhibitor CUDC-101 was used to investigate its potential as a targeted therapeutic agent for bladder cancer using chemosensitivity methods. (bvsalud.org)

a The high TET2 VAF levels (75%-90%) in panel C suggest that 1 copy of TET2 was probably deleted in the founding clone of this acute myeloid leukemia sample. (jamanetwork.com)

These findings suggest that the response to anti-PD-1 hinges on pre-existing anti-tumor immune response and that anti-PD-1 acts to free the CD8 T cells from inhibition to exert their anti-tumor activities [ 3 ]. (biomedcentral.com)
An inhibition of the normal development of hematopoietic cell elements occurs. (medscape.com)

Interestingly after dissociation into solitary cells and seeding onto new feeder cells the domed cells hardly ever formed smooth clones whereas the smooth cells created both domed and smooth clones. (researchhunt.com)

These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. (biomedcentral.com)
Developmental defects, including abnormalities in cloned fetuses and placentas, in addition to high rates of pregnancy loss and neonatal death have been encountered by every research team studying somatic cloning. (biomedcentral.com)
Dendrite pruning requires the developmentally regulated degradation of the cell adhesion molecule Neuroglian , and loss of Rab11 causes defects in the developmental degradation of Neuroglian and another target, the ion channel Ppk26 . (sdbonline.org)

We characterized a Thalictrum ortholog of the Antirrhinum majus transcription factor MIXTA-like2, responsible for conical cells, from three species with distinct floral morphologies, representing two pollination syndromes. (washington.edu)

Conical cells are present in sepals or stamens of insect-pollinated species, and in stigmas. (washington.edu)
However, somatic cloning has been inefficient in all species in which live clones have been produced. (biomedcentral.com)
Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie, this review will be focused on somatic cell cloning of cattle. (biomedcentral.com)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)

The malignant cells of acute lymphoblastic leukemia (ALL) are lymphoid precursor cells (ie, lymphoblasts) that are arrested in an early stage of development. (medscape.com)

Autologous USCs were obtained and characterized, and their capacity to differentiate into urothelial cells (UCs) and smooth muscle cells (SMCs) was tested. (biomedcentral.com)

Briefly, after electroporation of primary T cells with CXCR4 -targeting Cas9 ribonucleoproteins, single T cells are isolated on a chip and expanded into colonies. (nature.com)
The clones produced colonies in soft agar, demonstrating anchorage independence. (lsu.edu)
To distinguish and collect two morphologically unique cell populations for practical study in our work we suspended the dome-shaped colonies by softly pipetting up and down under a stereomicroscope. (researchhunt.com)

While archetypes are not consistently associated with specific whole-genome driver mutations, they are closely associated with subclonal populations at the single-cell level, indicating that subclones within a tumor often specialize in unique biological tasks. (edu.au)
We envisioned that enzyme-activatable cMOs could overcome these limitations, as the triggering enzymes could be expressed in individual cell populations using cis regulatory elements. (cdc.gov)

These cells were transiently transfected to express mtRFP (red) targeted to mitochondria to visualize their morphology. (nyu.edu)

Bovine coronavirus strain L9 (BCV-L9) and 5 wild-type isolates replicated in HRT-18 cells, inducing cell fusion. (lsu.edu)

Thus, in addition to molecules, cells use mechanical means to compete. (nature.com)
Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)

Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? (hpscreg.eu)

Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? (hpscreg.eu)
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? (hpscreg.eu)

We conducted this study to determine whether cell lines and eggs commonly used to isolate and propagate influenza viruses, poliovirus, and other human viruses can support productive replication of SARS-CoV-2. (cdc.gov)

The present data provide evidence that Snail promotes tumor cell motility and angiogenesis which is mainly mediated through the regulation of RhoA activity. (spandidos-publications.com)
Tumor cell motility is the hallmark of invasion and an essential step in metastasis. (spandidos-publications.com)
We studied tumor cell signaling in the cell subsets to understand the tumor-inhibitory potentials of EBF2. (amegroups.org)

Using this method, single-clone editing efficiencies, including the rate of mono- and bi-allelic indels or precise nucleotide replacements, can be assessed within 10 days from Cas9 ribonucleoprotein introduction in cells. (nature.com)

N2a-Rα cells expressed tyrosine hydroxylase (TH) mRNA as a functional marker of catecholaminergic neurotransmitter production. (biomedcentral.com)
PLA cells expressed multiple CD marker antigens similar to those observed on MSCs. (livingroom.health)

Clone 10 cells express a low level of GFP-Bax, which allows monitoring early MAC formation, corresponding to Bax translocation. (nyu.edu)

We isolated CSCs from low passage PDAC cell lines derived from human tumors and commercially available cell lines. (amegroups.org)
Periodontal ligament mesenchymal cells were obtained from two healthy human teeth (impacted third molars). (bvsalud.org)

Anatomy42

Organisms22

Diseases4

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment54

Phenomena and Processes77

Disciplines and Occupations6

Anthropology, Education, Sociology and Social Phenomena4

Information Science6

Health Care4

Genes14

Proliferation18

Plaque morphology4

Characterization2

Characteristics6

Proteins6

Biology5

Differentiation16

Pluripotency3

Proliferative3

Tissues7

Protein9

Molecular6

Progenitor cells2

Clonal5

Gene10

Growth kinetics1

Pluripotent9

Induce3

Fibroblasts10

Embryos3

Abstract2

Strains2

Maturation2

Transgenic1

Vitro6

Embryonic6

Fluorescence-activated cell2

Cellular1

IPSC3

Cultures6

Karyotype1

Vectors3

Epithelial cells4

Putative1

Regulate3

Expression12

Efficiency3

Stem5

Colony4

Therapeutic3

Acute myeloid1

Inhibition2

Feeder cells1

Defects3

Distinct1

Species4

Malignant cells1

Differentiate1

Colonies3

Populations2

Mitochondria1

Isolates1

Molecules2

Microbiological1

Embryo2

Propagate1

Tumor cell3

Single-clone1

Marker2

Formation1

Human2