Morphology cell line. Medical search. Frequent questions

Cell cultures containing undifferentiated stem cells were isolated from the primary tumor which have a euploid karyotype. (wikipedia.org)
Targeted clone R-Olig2 (like the other clones) retained pluripotency, typical hESC morphology, and a normal parental karyotype 46,XY. (ca.gov)
cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
The primary function of the red cell is to carry oxygen to tissue cells and to carry carbon dioxide away. (ivis.org)
The fluidity of normal red cells allow them to traverse tortuous capillary beds leading to close approximation of red cells with tissue cells. (ivis.org)
Metal nanoparticles able to bind diverse molecules to their surface was due to their relative surface area and size, making them ideal transport vehicles capable of penetrating cell and tissue barriers[ 2 ]. (ijpsonline.com)
Golgi staining is employed to study the morphology and connectivity of brain tissue from our animal models. (edu.au)
Reports from several laboratories suggest that a phenomenon termed epithelial-mesenchymal transformation, in which epithelial cells transform into fibroblasts capable of producing connective tissue matrix, is an important feature of tissue remodelling 10 , 11 . (ersjournals.com)
Sarcoma tissue samples and cell lines are subjected to a multiplatform genome-wide characterization of expression of protein-coding genes and microRNAs, DNA copy number changes, activating mutations, gene rearrangements and methylation profiling. (mskcc.org)
Hs 578T are epithelial cells isolated from breast tissue derived from a 74-year-old, White, female breast cancer patient. (atcc.org)
Instead, PICM-19H cells looked exclusively like hepatocytes, the main cell type that forms liver tissue. (usda.gov)
Migration or invasion of tumor cells into normal tissue is a principal cause of mortality and is thought to be a multi-factorial process, consisting of cell interaction with ECM and adjacent cells, as well as accompanying biochemical processes supportive of active cell movement. (bmj.com)

In this paper we report an altered cellular morphology in three ataxia-telangiectasia fibroblast lines, but not in a number of control fibroblast lines. (nih.gov)
MG-63, a cell that has fibroblast morphology isolated from the bone of a White, 14-year-old male patient with osteosarcoma. (atcc.org)

NRG1α was detected in serous cells within normal submucosal glands and in epithelial cells lining honeycombing areas in IPF, and was not detected in other patients. (bmj.com)
Conclusion Expression of SOX2 and MUC5B and lack of SPDEF in atypically differentiated cells of bronchiolised distal airspaces are consistent with abnormal programming of airway epithelial cells in IPF. (bmj.com)
Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)
Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)
Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
Furthermore, E1A DNA and protein persisted in epithelial cells in the lungs of these animals 9 . (ersjournals.com)
Here, we show that NSAIDs exacerbate CDI by disrupting colonic epithelial cells (CECs) and sensitizing cells to C. difficile toxin-mediated damage independent of their canonical role of inhibiting cyclooxygenase (COX) enzymes. (bvsalud.org)
The nanotube bundles are similar to the potential of nanotubes to induce genetic damage size of microtubules that form the mitotic spindle in normal lung cells, cultured primary and immor- and may be incorporated into the mitotic spindle talized human airway epithelial cells were apparatus. (cdc.gov)

Indeed, exposing aggregated P19 cells to dimethyl sulfoxide (DMSO) induces differentiation into cardiac and skeletal muscle. (wikipedia.org)
By aggregating the cells into an embryonic body, EC cells can also process differentiation. (wikipedia.org)
In P19 cells, addition of non-toxic concentrations of drugs to aggregated embryoid body cells can induce differentiation of P19 cells into specific cell lines depending on the added drug. (wikipedia.org)
Because the concentration of retinoic acid or DMSO is nontoxic to the cells, the drug-specific differentiation is due to induction of cells not selection. (wikipedia.org)
Mutants of P19 cells have been generated to investigate the mechanism of drug-specific differentiation. (wikipedia.org)
Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. (sigmaaldrich.com)
Early differentiation of newborn granule cells. (jneurosci.org)
The authors sought to identify mechanisms driving the differentiation of mucus cells during the bronchiolisation process. (bmj.com)
Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1α and NRG1β. (bmj.com)
In this work, pathways governing mucus cell differentiation were investigated in lung tissues from patients with IPF and other chronic pulmonary disorders. (bmj.com)
Neuregulin1α, which drives mucus cell differentiation in vitro, was expressed in normal airway submucosal glands and in lungs from patients with IPF. (bmj.com)
To identify potential therapeutic targets, our lab screens the genes and microRNAs in these signatures for involvement in proliferation, differentiation, and survival of sarcoma cell lines. (mskcc.org)
The rare cases that have neuroendocrine morphology are thought to arise from the neuroendocrine stem cells that are normally present in the prostate or from aberrant differentiation programs during cell transformation. (medscape.com)
The effect of exposure of different stem cells types to extremely low frequency magnetic fields on neuronal differentiation in relation to gene expression should be investigated. (emf-portal.org)
Additionally, tests were conducted with Egr1-knockdown and Egr1- overexpressing human bone marrow - mesenchymal stem cells as Egr1 was supposed to play a crucial role in magnetic field mediated neuronal differentiation . (emf-portal.org)
No differentiation and self-organization into multi-cellular bile ductules, as observed in the parental PICM-19 cell line, occurred within the PICM-19H cell monolayer even after six months of maintenance culture. (usda.gov)
While a few studies have attempted reprogramming of glioblastoma (GBM) cells toward a neuronal fate, this approach has not yet been used to induce differentiation into other lineages and in vivo data on reduction in tumorigenicity are limited. (lu.se)
Most importantly, forced differentiation substantially reduces tumorigenicity of GBM cells in an in vivo xenotransplantation model. (lu.se)
Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)

Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)

MA treatment was partially protective by decreasing Parkin expression, mtDNA and cytochrome c release and increasing cell viability. (cdc.gov)
Treatment of pancreatic and ovarian with different concentrations of silver nanoparticles inhibited the cell viability with half maximal inhibitory concentration values of 25.4 μg/ml and 23.9 μg/ml. (ijpsonline.com)
The objective of this study was to examine the influence of component of solid lipid nanoparticles on the viability of breast cancer cells using the MTT assay. (scialert.net)
H3.3 mutant cells exhibited significant sensitivity to NOTCH pathway inhibition and knockdown, resulting in decreased cell viability under treatment. (nature.com)

Despite the recent knowledge of the molecular mechanisms of corticosteroid drugs in lymphoid leukemia, information about the nanostructured surface morphology of the process of cell death is limited. (academicjournals.org)

In contrast, overexpression of USP19 increased cell invasiveness both in vitro and in vivo, further validating our findings. (nature.com)
An in vitro study was conducted to determine the effect of solid lipid nanoparticle on the human breast cancer cell lines (MCF-7 and MDA-MB231). (scialert.net)
Finally RhoE siRNA in the glioma cell line U251 inhibited cell invasion in vitro. (bmj.com)
Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

After six to nine days of treatment, the relative neuronal population declines, likely because of faster proliferation of non-neuronal cells. (wikipedia.org)
Human BeWo cell line has been used to study the effects of Δ-9-tetrahydrocannabinol (Δ9-THC) on the proliferation and gene transcription in the BeWo trophoblast cell line. (sigmaaldrich.com)
Leonotis nepetifolia mediated silver nanoparticles effectively reduced the proliferation of pancreatic and ovarian cancer cell lines than that of synthetic nanoparticles. (ijpsonline.com)
Recent studies have revealed that AXL contributes to leukaemic phenotypes through activation of oncogenic signalling pathways that lead to increased cell migration and proliferation. (cancerindex.org)
Therefore, this study proves that Rh4 inhibits cancer cell proliferation by activating the ROS/p53 signaling pathway and activating autophagy to induce ferroptosis, which provides necessary scientific evidence of the great anticancer potential of Rh4. (hindawi.com)
For the purposes of this article, the term "DCIS involving a papilloma" is used to describe an IDP involved by a cytologically malignant cell population, regardless of the size of the proliferation. (medscape.com)

Due to the pluripotency of P19 cells, those new derived cell lines can be ectoderm, mesoderm and endoderm-like cells. (wikipedia.org)

Cell motility is a complex process that requires post-translational regulation of a wide variety of proteins. (nature.com)
Proteins are essential for every process within a cell as well as cell-cell communication, including neurotransmission. (edu.au)
Human cell lines and primary cell culture (e.g. hippocampal cells from mutant mice) are a primary focus in our laboratory to study the role and interactions of disease-related proteins. (edu.au)
Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)

Studies have shown that a certain concentration of RA can induce P19 cells to differentiate into neuronal cells, including neurons and glial cells, whereas 0.5% - 1% DMSO led P19 cells to differentiate into cardiac or skeletal muscle cells. (wikipedia.org)
Treatment of undifferentiated P19 cells with retinoic acid can specifically induce them into neuronal cells. (wikipedia.org)
The test item did not induce a significant level of chromosome aberrations in Chinese hamster V79 cells in the performed experiments with and without metabolic activation. (europa.eu)

These stem cells were named embryonal carcinoma P19 cells. (wikipedia.org)
A targeted neuroglial reporter line generated by homologous recombination in human embryonic stem cells. (ca.gov)
We evaluated the protein expression level of RhoE in gliomas tissues, cell lines and brain tumor initiating stem cells (BTSCs). (bmj.com)
Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)

After each passage, PICM-19H cells grew to a nearly confluent monolayer of cells of hepatocyte morphology, i.e., cuboidal cells with centrally located nuclei joined by biliary canaliculi. (usda.gov)

Both exhibited cuboidal morphology and junctional complexes, but did not contain lamellar bodies or express surfactant protein A, B or C mRNAs. (ersjournals.com)
Ultrastructural analysis of the PICM-19B monolayers showed the roughly cuboidal cells displayed basal-apical polarization and were joined by tight junction-like complexes. (usda.gov)

Additionally, a number of ataxia-telangiectasia lymphoblastoid lines have been shown to have an unusual regulation of the cellular actin levels. (nih.gov)

The first human, trophoblastic endocrine cell type to be maintained in continuous culture. (sigmaaldrich.com)
The miR-217 inhibitor and mimics were transfected into human podocyte cells to investigate the pathological mechanism of miR-217 in this disease. (hindawi.com)
To identify novel molecular targets within the ubiquitination pathway that positively regulate migration we conducted a loss-of-function genetic screen using an epithelial cell line derived from human triple-negative breast cancer (MDAMB231) infected with a pooled shRNA interference library. (nature.com)
Therefore, we tested a hypothesis that organic dust extract (ODE) exposure induces mitochondrial stress using a human monocytic cell line (THP1). (cdc.gov)
A cytotoxicity study was conducted on two human cancer cell lines, pancreatic and ovarian, using sulforhodamine B assay. (ijpsonline.com)
Protein expression and function of organic anion transporters in short-term and long-term cultures of Huh7 human hepatoma cells. (cancerindex.org)
Human-derived hepatic cell lines are a valuable alternative to primary hepatocytes for drug metabolism, transport and toxicity studies. (cancerindex.org)
This is a hypotriploid human cell line. (atcc.org)
In this study, we targeted Olig2, a basic helix-loop-helix transcription factor that plays an important role in motoneuron and oligodendrocyte development, in human embryonic stem cell (hESC) line BG01 by homologous recombination. (ca.gov)
A novel human anti-AXL monoclonal antibody attenuates tumour cell migration. (cancerindex.org)
Here we report successful gene editing of H3.3K27M and G34R point mutations in human normal brain and pediatric glioma cells, generating pairs of otherwise isogenic cell lines that allowed us to define epigenetic and transcriptomic changes contributing to gliomagenesis in the native genomic context. (nature.com)
To study the transformative effects of oncohistone mutations in human pediatric gliomas in an otherwise isogenic context, we used CRISPR-Cas9 gene editing to revert H3.3K27M mutations in glioma cells back to wild type (WT) while in parallel introducing H3.3K27M and G34R point mutations into human astrocytes and H3.3WT glioma cells (Fig. 1a ). (nature.com)
A previous study with human breast cancer MCF-7 cells has shown that PFHxS is a weak agonist of estrogen receptor α (ERα) and a potential weak antagonist of the androgen receptor (AR) [10]. (researchsquare.com)
To this end, we overexpressed key transcriptional regulators of astroglial development in human GBM and GBM stem cell lines. (lu.se)
BACKGROUND: In utero exposure to human immunodeficiency virus (HIV) and antiretroviral (ART) is associated with adverse birth outcomes, which are often attributed to alterations in placental morphology. (bvsalud.org)
Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
As human osteosarcoma cells are a source of additional growth they did not affect osteoinduction. (bvsalud.org)
Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)

It has also been used to study placental transfer of highly purified non-dioxin-like polychlorinated biphenyls in BeWo cells. (sigmaaldrich.com)
This study used structural equation models (SEMs) to examine the impact of HIV and ART exposure on fetal growth outcomes and whether these associations are mediated by placental morphology in urban-dwelling Black South African women. (bvsalud.org)

The Mouse Lymphoma Assay was considered to be valid and to reflect the real potential of the test material to cause mutations in the cultured mouse cells used in this study. (europa.eu)
The cytotoxicity assay was done on HDFA, HeLa and HEK cell lines, haemolysis assay showed better haemocompatibility than CTAB. (ias.ac.in)

In order to identify novel molecular targets of tumor cell migration and invasion we performed a genetic screen with an shRNA library against ubiquitination pathway-related genes. (nature.com)
Results from RNA sequencing revealed that AEG‑1 modulated neuronal morphology and development by regulating the expression of numerous genes, such as ubiquitin C, C‑X‑C motif chemokine ligand 1, MMP9, Notch1, neuropilin 1 and ephrin type‑A receptor 4. (spandidos-publications.com)
Genes carry the fundamental information to build and maintain cells, including neurons. (edu.au)
Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
More recently, increased expression of NOTCH pathway genes was implicated in some DIPGs and NOTCH path inhibition contributed to reduced tumor growth in culture 14 , but the specificity of these treatments for H3.3 mutant cells remains unclear. (nature.com)
In K27M lines, the decrease of H3K27me3 was especially pronounced at super-enhancers of specific gene clusters, including the NOTCH pathway genes. (nature.com)

These cells were characterised by PCR, immunocytochemistry, electron microscopy, and Western and Northern blot analyses. (ersjournals.com)

Polarization is a fundamental cellular property that plays a vital role in various biological processes in multi-cellular as well as single-cell organisms. (elifesciences.org)
Transmission electron micrographs showed significant differences in cellular and organelle morphology upon ODE exposure. (cdc.gov)
Cell lines are fundamental to gain detailed information about disease-related pathways on a cellular level. (edu.au)
This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. (cancerindex.org)
The data indicated that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. (usda.gov)
These data indicated that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. (usda.gov)
Direct cellular reprogramming has recently gained attention of cancer researchers for the possibility to convert undifferentiated cancer cells into more differentiated, postmitotic cell types. (lu.se)
Higher-grade lesions are more cellular, with atypical cells and abnormal mitoses. (medscape.com)

Any gene of a cell can undergo changes in the cells lifetime, i.e. it can become mutated. (edu.au)
During mitosis, cells undergo symmetrical cell division, while oocyte meiotic maturation undergoes two consecutive, asymmetric divisions that generate a totipotent haploid oocyte and two small polar bodies not involved in DNA replication. (bioone.org)
Treated cells undergo a remarkable shift in structure, acquiring an. (lu.se)
Treated cells undergo a remarkable shift in structure, acquiring an astrocyte-like morphology with star-shaped bodies and radial branched processes. (lu.se)

However, researchers utilize these cells to study the development of cancer cells in order to find more specific treatments. (wikipedia.org)
Thus, this reconstruction system provides an experimental paradigm to study features of the assembly process and structure of Par-dependent cell-autonomous polarity. (elifesciences.org)
The study included assessments of cell growth rate, their morphological features, and their liver detoxification functions. (usda.gov)
The study included assessments of growth rate and cell density in culture, morphological features, and hepatocyte detoxification functions, i.e., inducible P450 activity, ammonia clearance, and urea production. (usda.gov)

P19 cells can be maintained in exponential growth because of a stable chromosomal composition. (wikipedia.org)
In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
Keep flask in a vertical position until the cells reach the exponential phase of growth. (sigmaaldrich.com)
To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. (sigmaaldrich.com)
Glioma lines that were edited to correct the pathogenic H3F3A mutation exhibited less invasiveness and growth in xenografted mice than their mutated counterparts. (nature.com)

The molecular biology underlying renal cell carcinoma (RCC) development and progression has been a key milestone in the management of this type of tumor. (cancerindex.org)

Using doses between 1 μM to 3 μM of RA can generate neurons as the most abundant cell type. (wikipedia.org)
Consequently, a particular type of a curved-line bending compliant mechanism is developed with the ability to reduce some of the deformation constraints, specifically the need to fold in an elastic-kinetic structure. (researchgate.net)
RNA Sanger sequencing analysis was performed in AEG‑1‑deficient HT22 cells and wild‑type HT22 cells without knockout (KO). (spandidos-publications.com)
Ferroptosis is a novel type of regulated cell death caused by iron-dependent lipid peroxidation [ 26 ]. (hindawi.com)

An angiosarcoma (AS) is an uncommon malignant neoplasm characterized by rapidly proliferating, extensively infiltrating anaplastic cells derived from blood vessels and lining irregular blood-filled spaces. (medscape.com)
In its more aggressive form, irregular sheets of anaplastic cells may have only poorly defined vascular channels and may be difficult to differentiate from anaplastic melanomas and carcinomas. (medscape.com)

The new cells are young and morphologically distinct (large, polychromatophilic - see morphology section). (ivis.org)
Morphologically, PICM-19B cells resembled PICM-19H cells except that they did not have the special cell-to-cell unions found between PICM-19H cells that are characteristic of liver cells. (usda.gov)

there is a general lack of eggplant experimental populations, such as recombinant inbred lines (RILs), advanced backcrosses (ABs), introgression lines (ILs) or multiparent advanced generation intercross (MAGIC) populations. (frontiersin.org)

Cyclospora and Cryptosporidium are examples of coccidian parasites that infect the epithelial (lining) cells of the intestinal tract. (cdc.gov)

miR-193b-3p could also be a predictive biomarker in chronic kidney disease undergoing radical nephrectomy for renal cell carcinoma [ 20 ]. (hindawi.com)
Clear cell renal cell carcinoma (ccRCC) is the seventh most frequently diagnosed tumor in adults in Europe and represents approximately 2.5% of cancer deaths. (cancerindex.org)

The modal chromosome number was 66 occurring in 44% of cells. (atcc.org)

Reciprocal editing of cells generally produced reciprocal effects on tumorgenicity in xenograft assays. (nature.com)

After 10 days of exposure, astroglial cells can be detected using glial fibrillary acidic protein (GFAP), which is a specific marker of glial cells. (wikipedia.org)

Cancer cells in humans may result in the patient's death if the aggressive cancer cell grows and metastasizes. (wikipedia.org)

Red blood cells (RBC) are produced in the bone marrow. (ivis.org)
Effete red cells are phagocytized and metabolized by the macrophages of spleen, bone marrow, and liver. (ivis.org)

AgNPs were toxic to the cells, which can lead to damage of deoxyribonucleic acid, generation of reactive oxygen species, mitochondrial damage and apoptotic induction[ 7 , 8 ]. (ijpsonline.com)

Moreover, signaling pathways related to neurogenesis and myogenesis were also investigated by studying gene expression or generating mutants of P19 cells. (wikipedia.org)
The red cell also has metabolic pathways (hexose monophosphate shunt and methemoglobin reductase) which protect hemoglobin from oxidation. (ivis.org)

P19 cells is an embryonic carcinoma cell line derived from an embryo-derived teratocarcinoma in mice. (wikipedia.org)
Also, it is the most characterized embryonic carcinoma (EC) cell line that can be induced into cardiac muscle cells and neuronal cells by different specific treatments. (wikipedia.org)
Because embryonal carcinoma can differentiate into cells of all three germ layers, P19 cells can also differentiate into those ectoderm, mesoderm and endoderm-like cells. (wikipedia.org)
When embryonal carcinoma cells are cultured at high density, they start to differentiate. (wikipedia.org)

We identified the deubiquitinase USP19 and demonstrated that its silencing reduces the migratory and invasive potential of highly invasive breast cancer cell lines. (nature.com)
The cytotoxicity of the components of the SLN formulation was low: SLN with 1% oleyl alcohol displayed no significant cytotoxicity effect on breast cancer cells. (scialert.net)
Use these cells in breast cancer research. (atcc.org)

Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)

By assessing the mitochondria respiration we found evidence proposing IU1 inhibits complex I. Our results suggests that IU1 activates AMPK in certain cell lines with the possible underlying mechanism being the inhibition of complex I of the respiratory chain. (helsinki.fi)
The PICM-19H cells contained numerous mitochondria, Golgi apparatus, smooth and rough endoplasmic reticulum, vesicular bodies and occasional lipid vacuoles. (usda.gov)
Our results demonstrate that NSAIDs exacerbate CDI by synergizing with C. difficile toxins to damage host cell mitochondria. (bvsalud.org)

These cells also stain positive for α-napthhyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). (sigmaaldrich.com)

Morphological parameters such as height, cell. (academicjournals.org)

Mucus cells lining abnormal airways from patients with IPF expressed MUC5B and SOX2 but lacked SAM pointed domain containing ETS transcription factor (SPDEF) and forkhead box A3(FOXA3). (bmj.com)

Other than into neurons and astrocytes, P19 cells can also differentiate to oligodendrocytes, which can be detected using the specific markers, myelin-associated glycoprotein and 2',3'-Cyclic-nucleotide 3'-phosphodiesterase. (wikipedia.org)
H , Graph of the mean ± SEM voltage-dependent sodium current amplitude of eGFP-positive neurons increased rapidly toward those of preexisting cells. (jneurosci.org)

Alteration or exacerbation of malignant tumor cell migration and dissemination is the principal cause of death due to solid tumors 5 . (nature.com)

Because of such association between Par-dependent polarization and other processes, the Par-complex exhibits different behavioral characteristics in an individual context, making it difficult to determine general features of the dynamic process taking place during cell polarization by the Par-complex. (elifesciences.org)
To understand the general and dynamic characteristics of the cell polarization process induced by the Par-complex, we attempted to reconstruct Par-complex-dependent cell polarization system in a cell-autonomous manner using non-polar cells. (elifesciences.org)

In AST, AEG-1 causes glutamatergic excitotoxicity by downregulating the activity of the excitatory amino acid transporter 2 (EAAT2) promoter, leading to neuronal cell death in glioma-induced neurodegenerative disease ( 10 ). (spandidos-publications.com)
To identify major effectors, we used CRISPR-Cas9 to introduce H3.3K27M and G34R mutations into previously H3.3-wildtype brain cells, while in parallel reverting the mutations in glioma cells back to wildtype. (nature.com)
More recently, CRISPR-mediated knockout of the mutant H3F3A allele in two H3.3K27M glioma lines restored more normal histone mark deposition and reduced tumorigenesis 9 . (nature.com)

Tumor cell dissemination in cancer patients is associated with a significant reduction in their survival and quality of life. (nature.com)
Currently, nanobiotechnology is the growing science in the field of medicine employing silver nanoparticles to deliver drugs to target cancer cells and proved to be potential anticancer agents. (ijpsonline.com)
AEG-1 has been shown to localize to the cell membrane, cytoplasm, endoplasmic reticulum, nucleolus and nucleus in cancer cells ( 2 ). (spandidos-publications.com)
Prostate cancer develops when cell division and cell death rates cease being equal. (medscape.com)
The critical challenge in cancer treatment is how to effectively kill cancer cells without affecting healthy cells. (hindawi.com)
Rho family members are known to regulate malignant transformation motility and invasion of cancer cells, but the clinicopathological significance of Rnd3 remains unclear. (bmj.com)
We take advantage of the similarity between neural developmental processes and cancer hierarchy to mitigate, if not completely abolish, the malignant nature of tumor cells and pave the way for new intervention strategies. (lu.se)

More importantly, we demonstrated that USP19 catalytic activity is important for the control of tumor cell migration and invasion, and that its molecular mechanism of action involves LRP6, a Wnt co-receptor. (nature.com)
These data are then used to identify both genetically distinct subtypes of sarcoma and molecular signatures associated with tumor morphology, grade, recurrence, and survival. (mskcc.org)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)

The cell line is pluripotent and can differentiate into cell types of all three germ layers. (wikipedia.org)

Consistent with previous reports on rodents, early GFP-expressing cells appeared biased to a neuronal fate, whereas late GFP-expressing cells appeared biased to an oligodendrocytic fate. (ca.gov)

Also, exposing P19 cells to retinoic acid (RA) can differentiate them into neuronal cells. (wikipedia.org)
The difference between these subtype cell lines is the ability to differentiate into neuronal cells or muscle cells in response to treatment with retinoic acid or DMSO, respectively. (wikipedia.org)
Most importantly, GFP expression recapitulated endogenous Olig2 expression when R-Olig2 was induced by sonic hedgehog and retinoic acid, and GFP-positive cells could be purified by fluorescence-activated cell sorting. (ca.gov)

Radionuclide-tagged red blood cell scanning is helpful in the differential diagnosis with other multifocal vascular processes of bone and multiple myeloma or Langerhans cell histiocytosis. (medscape.com)

The ubiquitination pathway plays a fundamental role in the maintenance of protein homeostasis both in normal and stressed conditions and its dysregulation has been associated with malignant transformation and invasive potential of tumor cells, thus highlighting its value as a potential therapeutic target. (nature.com)
These will provide the ability to evaluate and compare data from a number of different cell lines which will facilitate greater insight into the cause of the ASDs and will be extremely useful for uncovering new therapeutic and diagnostic targets. (topsan.org)

Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)

Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
Add thawed cells to a conical based centrifuge tube, e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. (sigmaaldrich.com)
The PICM-19B cell line was derived from cells that spontaneously appeared in the parental PICM-19 cell culture. (usda.gov)

This cell line is a suitable transfection host. (atcc.org)

Anatomy38

Organisms13

Diseases31

Chemicals and Drugs68

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Phenomena and Processes66

Disciplines and Occupations1

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Tissue17

Fibroblast2

Epithelial Cells9

Differentiation19

Adherent2

Viability4

Surface morphology1

Vitro5

Proliferation6

Pluripotency1

Proteins5

Induce3

Stem cells4

Monolayer1

Cuboidal2

Lymphoblastoid1

Human18

Placental2

Assay2

Genes6

Electron microscopy1

Cellular8

Undergo4

Study4

Growth5

Biology1

Type4

Anaplastic cells2

Morphologically2

Recombinant1

Intestinal1

Renal cell carc2

Modal1

Xenograft1

Glial cells1

Humans1

Bone marrow2

Induction1

Pathways2

Carcinoma4

Breast3

Cultures3

Mitochondria3

Erythrocytes1

Morphological1

Abnormal1

Neurons2

Malignant tumor1

Characteristics2

Glioma3

Cancer7

Molecular3

Pluripotent1

Neuronal fate1

Retinoic acid3

Multiple myeloma1

Therapeutic2

IPSC1

Culture5

Transfection1