Monomeric inhibit stable bind domains single domain antibodies. Medical search. Frequent questions

All variants bound the PfRH5 receptor basigin and were recognized by a panel of monoclonal antibodies. (nature.com)
For the last two decades, our understanding of immunology exploded with the advent of technologies that allowed high throughput screening and generation of large molecule biologics such as monoclonal antibodies (mAb) and small molecule compounds. (frontiersin.org)

On the way to activation, disassembly of the SH3-SH2- kinase core opens a new autoinhibitory site on the kinase domain for PHTH domain binding that is ultimately released upon interaction of PHTH with PIP3. (elifesciences.org)
Membrane-induced dimerization activates BTK and we present here a crystal structure of an activation loop swapped BTK kinase domain dimer that likely represents the conformational state leading to trans-autophosphorylation. (elifesciences.org)
Together, these data provide the first structural elucidation of full-length BTK and allow a deeper understanding of allosteric control over the BTK kinase domain during distinct stages of activation. (elifesciences.org)
The TEC and SRC kinases share the SH3-SH2-kinase domain arrangement (the 'Src module') ( Fig. 1a ). (elifesciences.org)
Structures of the autoinhibited SRC family kinases were solved in 1997 revealing, for the first time, the compact arrangement of the SH3 and SH2 domains assembled onto the distal side of the catalytic kinase domain [ 4 ]. (elifesciences.org)
Current therapeutics target EGFR's kinase domain or extracellular region. (biorxiv.org)
We propose that the resulting disruption of native TM interactions disrupts the conformation of the kinase domain, inhibiting EGFR's ability to send migratory cell signals. (biorxiv.org)
However, recent studies have suggested that BRAFi/MEKi and ERK1/2i resistance can arise through activation of a parallel signalling pathway leading to activation of ERK5, an unusual protein kinase that contains both a kinase domain and a transcriptional transactivation domain. (babraham.ac.uk)

A single-domain antibody is a peptide chain of about 110 amino acids long, comprising one variable domain (VH) of a heavy-chain antibody, or of a common IgG. (wikipedia.org)
RAGE binds a wide array of ligands including advanced glycation end products, amyloid β-peptide, S100 family proteins, and high mobility group Box 1 (HMGB1). (elifesciences.org)
The protease not only releases small peptides, such as the amyloid-β peptide, which drives Alzheimer's disease pathogenesis, but also intracellular domains, which can have critical functions in nuclear signaling. (cipsm.de)
Most of the liquid state NMR data, obtained on monomeric Ab, indicate that the peptide by itself is dynamically flexible in solution. (alzforum.org)
Our lab has recently developed a new strategy to regulate RTK activity by designing a peptide that specifically binds to the transmembrane (TM) region of the RTK to allosterically modify kinase activity. (biorxiv.org)

Cartilaginous fishes also have heavy-chain antibodies (IgNAR, 'immunoglobulin new antigen receptor'), from which single-domain antibodies called VNAR fragments can be obtained. (wikipedia.org)
In both cases, the long half-life is due to their molecular size above the renal clearance threshold and their interaction with a membrane-bound receptor named the neonatal Fc receptor (FcRn). (nature.com)
Proteins that do not bind the receptor are directed to lysosomal degradation. (nature.com)
In contrast, the intestinal SALSA was more enriched with the scavenger receptor cysteine-rich domains. (researchgate.net)
MUC16 decreases TRAIL receptor R2 (DR5) expression and inhibits pro-caspase-8 activation at the death-inducing signaling complex (DISC). (biomedcentral.com)
BTK, a TEC-family tyrosine kinase activated by the B-cell antigen receptor, contains a variety of regulatory domains and it is subject to complex regulation by membrane phospholipids, protein ligands, phosphorylation, and dimerization. (elifesciences.org)
A particle according to claim 1 or 2, wherein the heterologous polypeptide has specific binding affinity for a cognate receptor on die surface of an eukaryotic cell, binding to which does not allow infection of the cell by the viral particle. (sumobrain.com)
A particle according to any one of the preceding claims, wherein the heterologous polypeptide sterically hinders binding of d e viral glycoprotein to its cognate receptor on the eukaryotic cell. (sumobrain.com)
A particle acording to claim 11 , wherein the protease cleavage site becomes accessible after the heterologous polypeptide has bound to its cognate receptor on the eukaryotic cell. (sumobrain.com)
Injectable polymer-nanoparticle (PNP) hydrogels are leveraged as a depot technology for the sustained delivery of a nanoparticle antigen (RND-NP) displaying multiple copies of the SARS-CoV-2 receptor-binding domain (RBD) and potent adjuvants including CpG and 3M-052. (uw.edu)
Here, we show that the African Rhinolophus bat clade 3 sarbecovirus PRD-0038 S has a broad angiotensin-converting enzyme 2 (ACE2) usage and that receptor-binding domain (RBD) mutations further expand receptor promiscuity and enable human ACE2 utilization. (uw.edu)
We determine a cryo-EM structure of the PRD-0038 RBD bound to Rhinolophusalcyone ACE2, explaining receptor tropism and highlighting differences with SARS-CoV-1 and SARS-CoV-2. (uw.edu)

A single-domain antibody can be obtained by immunization of dromedaries, camels, llamas, alpacas or sharks with the desired antigen and subsequent isolation of the mRNA coding for the variable region (VNAR and VHH) of heavy-chain antibodies. (wikipedia.org)
Other specific initiatives were conducted such as evaluation of the sublingual route for immunization, investigation of the Principal Immunodominant Domain (PID) of gp41, and direct HIV inhibition with an Ag family which demonstrated in vitro antiviral activities comparable to the antiretroviral drug T20 (Fuzeon). (europa.eu)
Analysis following immunization of rabbits identified quantitative and qualitative differences in terms of the functional IgG antibody response against the P. falciparum parasite. (nature.com)
In this work, a single immunization SARS-CoV-2 subunit vaccine is developed that can rapidly generate potent, broad, and durable humoral immunity. (uw.edu)
Additionally, these single-immunization hydrogel-based vaccines elicit potent and consistent neutralizing responses. (uw.edu)

With a molecular weight of only 12-15 kDa, single-domain antibodies are much smaller than common antibodies (150-160 kDa) which are composed of two heavy protein chains and two light chains, and even smaller than Fab fragments (~50 kDa, one light chain and half a heavy chain) and single-chain variable fragments (~25 kDa, two variable domains, one from a light and one from a heavy chain). (wikipedia.org)
The overall objective of EuroNeut-41 was to design a vaccine that raises antibodies able to prevent HIV cell fusion by blocking the virus fusion protein gp41 in its pre-fusogenic conformation. (europa.eu)
FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)
The Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) has recently emerged as a leading candidate antigen against the blood-stage human malaria parasite. (nature.com)
Here we report the production of full-length PfRH5 protein using a cGMP-compliant platform called ExpreS 2 , based on a Drosophila melanogaster Schneider 2 (S2) stable cell line system. (nature.com)
The antibodies induced by one protein variant were shown to be qualitatively similar to responses induced by other vaccine platforms. (nature.com)
Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). (cipsm.de)
The MUC16CTD anchors the protein at the cell surface and consists of a 229 amino acid extracellular region containing a potential proteolytic cleavage site, a 23 residue transmembrane domain, and a 31 amino acid cytoplasmic tail. (biomedcentral.com)
Rab40c is a SOCS box-containing protein which binds Cullin5 to form a ubiquitin E3 ligase complex (Rab40c/CRL5) to regulate protein ubiquitylation. (life-science-alliance.org)
Mechanistically, we found that Rab40c binds the protein phosphatase 6 (PP6) complex and ubiquitylates one of its subunits, ankyrin repeat domain 28 (ANKRD28), thus leading to its lysosomal degradation. (life-science-alliance.org)
This method entails affinity maturation of a portion of a first or secondary antibody for a target protein. (patentpc.com)
Additionally, we observed crucial TMPK dynamics brought on by ligand-binding and identified key residues such as Phe68 and Tyr101 as the critical points of the protein-ligand interaction. (bvsalud.org)
We have also identified critical amino acids and monosaccharides (Gal and GlcNAc) that contributed significantly to the protein-ligand binding through the per-residue decomposition of binding free energy. (bvsalud.org)
A monoclonal antibody raised against purified flagellar basal apparatuses from the green flagellate Spermatozopsis similis reacted with a protein of 210 kDa (p210) in western blots. (lookformedical.com)
Using a polyclonal antibody (anti-p210) raised against the C-terminal part of p210, it was shown that the protein was highly enriched in the basal apparatuses. (lookformedical.com)
Lipoate protein ligase B primarily recognizes the C8-phosphopantetheine arm of its donor substrate and weakly binds the acyl carrier protein. (du.ac.in)
Polypyrimidine tract binding protein and associated splicing factor (PSF) has diverse functions inside the cell such as transcriptional regulation, DNA repair, acts as nucleic acids binding protein and regulate replication and infectivity of different viruses. (biomedcentral.com)
The protein binding study identified the association of host protein PSF with HIV-1 integrase. (biomedcentral.com)
In the present study, we have identified a new HIV-1 integrase interacting protein, polypyrimidine tract binding protein and associated splicing factor (PSF) through pull down assay and co immunoprecipitation of IN with mammalian cell protein. (biomedcentral.com)

Numerous monoclonal therapeutic and diagnostic monoclonal antibody (MAbs), are used in clinical settings to diagnose and treat human diseases such as cancer and autoimmune disorders. (patentpc.com)

germline segments for the nucleic acids molecule encoding variable light chain of first antibody. (patentpc.com)
One example of this method is to produce a plurality modified antibodies by producing nucleic acids molecules that encode modified forms or light chains of the first antibody. (patentpc.com)

Human single-domain antibodies targeting various tumor antigens including mesothelin, GPC2 and GPC3 were isolated by phage display. (wikipedia.org)

Anti-merozoite vaccine studies have long relied on the standardized in vitro assay of growth inhibition activity (GIA) 12 , whereby purified IgG antibodies are tested against parasites cultured in human red blood cells (RBC) in the absence of other cell types. (nature.com)
The synthesis and applications of the peptides are gaining increasing popularity as a result of the developments in biotechnology and bioengineering areas and for a number of research purposes including cancer diagnosis and treatment, antibiotic drug development, epitope mapping, production of antibodies, and vaccine design. (intechopen.com)
With this information, I hope to design a template for a vaccine that can teach our bodies to make HIV broadly neutralizing antibodies from scratch and protect us against HIV diversity. (wrfseattle.org)
PRD-0038 S vaccination elicits greater titers of antibodies cross-reacting with vaccine-mismatched clade 2 and clade 1a sarbecoviruses compared with SARS-CoV-2 S due to broader antigenic targeting, motivating the inclusion of clade 3 antigens in next-generation vaccines for enhanced resilience to viral evolution. (uw.edu)

They are easily isolated using the same phage panning procedure used for antibodies, allowing them to be cultured in vitro in large concentrations. (wikipedia.org)

Thus, HERA is a novel cellular assay that can be used to predict how FcRn-binding proteins are rescued from intracellular degradation. (nature.com)
The major antibody class found in the blood, IgG, has a remarkable persistence, with a serum half-life of 20-23 days, compared with only hours or a few days for other circulating proteins 5 . (nature.com)
These proteins contain two related domains known as the chromo- and the chromoshadow-domain. (cipsm.de)
The reason why Peter Lansbury and others used the term 'natively unfolded' for a-synuclein and other proteins, especially peptides, is that they do not exist, to a significant degree, in the same kind of stable structure that larger native proteins like myoglobin do. (alzforum.org)

Furthermore, differential binding was observed also to known endogenous ligands C1q, mannose-binding lectin, and secretory IgA. (researchgate.net)
To mediate these processes, the extracellular ligand binding region of EGFR senses environmental cues via interactions with one of its 7 known ligands, of which epidermal growth factor (EGF) is the most well characterized ( 2 , 3 ). (biorxiv.org)

Our findings suggest that it should be possible to block RAGE signaling by inhibiting HS-RAGE interaction. (elifesciences.org)
By inhibiting HS-RAGE interaction genetically and pharmacologically, our work validated an alternative strategy to antagonize RAGE. (elifesciences.org)

Alternatively, single-domain antibodies can be made from common murine, rabbit or human IgG with four chains. (wikipedia.org)
The lack of binding of mIgG to hFcRn explains why murine antibodies are rapidly removed from the circulation in humans, despite long half-life in mice 32 . (nature.com)

Although most research into single-domain antibodies is currently based on heavy chain variable domains, Nanobodies derived from light chains have also been shown to bind specifically to target epitopes. (wikipedia.org)
Camelid Nanobodies have been shown to be just as specific as antibodies, and in some cases they are more robust. (wikipedia.org)

The most promising Ags were submitted to a more in-depth biophysical investigation of their structure and conformation stability, and for their capacity to interact with neutralising antibodies (Abs) and peptides. (europa.eu)

These peptides have similar affinity to antigens as whole antibodies, but are more heat-resistant and stable towards detergents and high concentrations of urea. (wikipedia.org)
If affinity can be retained, the single-domain antibodies can likewise be produced in E. coli, S. cerevisiae or other organisms. (wikipedia.org)
The affinity matured antibodies are more or less binding to a target antibody. (patentpc.com)
This article aims to offer methods for optimizing and enhancing the binding affiniites and selecting antibodies with desired affinity. (patentpc.com)
This document describes methods for affinity maturation of antibodies and fragments of them based on structure/activity relation (SAR). (patentpc.com)
The affinity matured starting antibody has a higher binding specificity and affinity than the target antigen. (patentpc.com)
The molecular mechanics generalized Born surface area (MM/GBSA) calculations yielded the highest binding affinity for LNFPI(ß)/P[6] (-13.93 kcal/mol) due to the formation of numerous hydrogen bonds between VP8* and HBGAs. (bvsalud.org)
A particle according to claim 1 or 2, wherein the heterologous polypeptide has no specific binding affinity for a eukaryotic cell surface component. (sumobrain.com)

This includes antibodies that have the ability to modulate a target's functional activity and/or antibodies that are more bioavailable or stable in specific cellular and tissue environments. (patentpc.com)

Moreover, the kinetic constraints imposed by such rapid erythrocyte invasion mean that extremely high concentrations of functional antibody are required to neutralize the parasite 6 . (nature.com)

The ligand-bound TM configuration promotes asymmetric dimerization of the intracellular juxta-membrane (JM) and kinase domains, which causes autophosphorylation of intracellular tyrosine residues ( 6 , 7 ). (biorxiv.org)
We show here that photosynthesis is stimulated by a novel, intracellular alpha-CA bound to the chloroplast thylakoids. (lookformedical.com)

GRFT can inhibit human immunodeficiency virus (HIV) infection at picomolar concentrations, surpassing the ability of most anti-HIV agents. (mdpi.com)

Furthermore, both the mouse and human receptors bind more strongly to mouse serum albumin (MSA) than to HSA. (nature.com)

This has led to the development of a range of ERK1/2 inhibitors (ERKi) that either inhibit kinase catalytic activity (catERKi) or additionally prevent the activating pT-E-pY dual phosphorylation of ERK1/2 by MEK1/2 (dual-mechanism or dmERKi). (babraham.ac.uk)

Blood-stage vaccines seek to induce antibodies against the merozoite form of the parasite that invades erythrocytes 2 , and could complement pre-erythrocytic immunity afforded by RTS,S/AS01, protect against disease severity and/or reduce transmission by accelerating the control and clearance of blood-stage parasitemia. (nature.com)
My job is to learn from the patients' samples what is required to induce our immune system to make these super-effective antibodies. (wrfseattle.org)

A single-domain antibody (sdAb), also known as a Nanobody, is an antibody fragment consisting of a single monomeric variable antibody domain. (wikipedia.org)

They further validate the idea that this knock-in mouse, which preserves expression and binding of RAGE, may be a valuable model for studying this important molecule in disease pathogenesis. (elifesciences.org)
A method based on atomic force microscopy is used to delineate the properties that determine single-molecule adhesion onto solid substrates in aqueous environment. (cipsm.de)

The method identifies a similar antibody or portion of an antibody that has a lower activity against the target antigen than its corresponding form. (patentpc.com)
Characterization of PRD-0038 S using cryo-EM and monoclonal antibody reactivity reveals its distinct antigenicity relative to SARS-CoV-2 and identifies PRD-0038 cross-neutralizing antibodies for pandemic preparedness. (uw.edu)

The RAGE mutant demonstrated normal ligand-binding but impaired capacity of HS-binding and oligomerization. (elifesciences.org)
The authors report that knock-in mice, with RAGE is mutated at sites responsible for heparan sulfate binding and oligomerization, phenocopy RAGE knockout mice. (elifesciences.org)
Using in vitro and cellular techniques, we present a mVP40 assembly model highlighting two distinct oligomerization interfaces: the (N-terminal domain (NTD) and C-terminal domain (CTD)) in mVP40. (uci.edu)
The AF form of SALSA had a more intact structure and contained peptides from the zona pellucida domain, which is involved in cell differentiation and oligomerization. (researchgate.net)
Ligand binding promotes EGFR oligomerization mediated by the extracellular region. (biorxiv.org)

Linker regions and domains are labeled, residue numbering shows BTK domain boundaries. (elifesciences.org)
The target region is altered by replacing a single amino acids residue. (patentpc.com)

In contrast to common antibodies, two out of six single-domain antibodies survived a temperature of 90 °C (194 °F) without losing their ability to bind antigens in a 1999 study. (wikipedia.org)
Camelid and fish derived sdAbs are able to bind to hidden antigens that are not accessible to whole antibodies, for example to the active sites of enzymes. (wikipedia.org)

Griffithsin's antiviral activity stems from its ability to bind terminal mannoses present in high-mannose oligosaccharides and crosslink these glycans on the surface of the viral envelope glycoproteins. (mdpi.com)

The DEC-205 monomer forms a compact structure comprising two intercalated rings of C-type lectin-like domains, where the N-terminal cysteine-rich and fibronectin domains reside at the central intersection. (uci.edu)

The method also compares the amino acids sequences of the variable heavy and variable light chains of the first antibody to those of the corresponding variable heavy or variable light chains of the related antibody. (patentpc.com)
This is where the target region refers to a portion of the first antibody that has at least one amino acids difference from the same area in the related antibody. (patentpc.com)
This ensures that each modified antibody contains an amino acid replacement to a different amino Acid than the first. (patentpc.com)

Progress in this arena, however, is being made with a new generation of merozoite antigen targets identified in recent years that exhibit relatively low levels of polymorphism and against which functional neutralizing antibodies can be raised by vaccination. (nature.com)

A particle according to any one of the preceding claims, wherein heterologous polypeptide sterically hinders fusion of an enveloped viral particle with an eukaryotic cell to which it is bound. (sumobrain.com)
Molecular docking study supported the argument that PSF hinders the binding of viral DNA with IN. (biomedcentral.com)

These methods allow for the optimization of antibodies that have higher and better activity (e.g. (patentpc.com)
Optimization and Identification of Single mutation in Hemoglobin Variants with 2,2,2 trifluoroethanol modified digestion method and nano-LC coupled MALDI MS/MS. Molecules. (du.ac.in)

Screening techniques like phage display and ribosome display help to identify the clones binding the antigen. (wikipedia.org)

Precisely how the BTK N-terminal domains (the Pleckstrin homology/Tec homology (PHTH) domain and proline-rich regions (PRR) contain linker) contribute to BTK regulation remains unclear. (elifesciences.org)
5 ]. The three domains (SH3, SH2, kinase), the SH2-kinase linker, the activation loop and the active site are labeled. (elifesciences.org)

An alternative approach is to split the dimeric variable domains from common immunoglobulin G (IgG) from humans or mice into monomers. (wikipedia.org)
Humans occasionally produce single domain antibodies by the random creation of a stop codon in the light chain. (wikipedia.org)

Compared to a clinically relevant prime-boost regimen with soluble vaccines formulated with CpG/alum or 3M-052/alum adjuvants, PNP hydrogel vaccines more rapidly generated higher, broader, and more durable antibody responses. (uw.edu)

Specifically, the TM of unliganded (inactive) EGFR dimerizes at the C-terminus (C t ), while the ligand bound form dimerizes N-terminally (N t ), and the two helices are also rotated by 180° between the conformations ( 4 , 5 ). (biorxiv.org)

The potential to inhibit other viruses as well as parasites has also been demonstrated. (mdpi.com)

This means that the related antibody contains a related varible heavy chain, or a comparable variable light chains. (patentpc.com)
Once a target area has been identified, modified antibodies are made that contain a variable heavy and variable light chains, or a portion thereof. (patentpc.com)

This antibody blocks RAGE signaling in vitro and in vivo, recapitulating the phenotype of Ager AHA/AHA mice. (elifesciences.org)

Another design strategy was developed using gammaretroviruses backbone where 2 domains were substituted with HIV epitopes in the N-terminal part of the ectodomain and the membrane proximal external region (MPER) respectively. (europa.eu)

To test this, we generated a monoclonal antibody that targets the HS-binding site of RAGE. (elifesciences.org)

LNnT binds more strongly to P[11] (-12.88 kcal/mol) than LNT (-4.41 kcal/mol), suggesting a single change in the glycan linkage might impact its binding profile significantly. (bvsalud.org)

The single domain antibodies including VNARs can be humanized for clinical applications. (wikipedia.org)

8 h following single oral doses of the compounds was associated with significant losses of radiolabelled proline and 4-hydroxyproline from collagen during this period. (embl.de)
We successfully identified novel compounds that may serve as potential building blocks for constructing contemporary antivirals against MPXV and highlighted the molecular mechanisms underlying their binding with TMPK. (bvsalud.org)

Single gas permeation through SAPO-34 membranes decreased with molecular size for H 2 , CO 2 , N 2 , CH 4 , and n-C 4 H 10 . (mrs.org)

De novo formation of an adherens junction (AJ) between single epithelial cells requires coordinated, spatial actin dynamics, but the mechanisms steering nascent actin polymerization for cell-cell adhesion initiation are not well understood. (rupress.org)

Single-domain antibodies are being researched for multiple pharmaceutical applications, and have potential for use in the treatment of acute coronary syndrome, cancer, Alzheimer's disease, and Covid-19. (wikipedia.org)

Recently, Np95 (also known as UHRF1 or ICBP90) has been found to interact with Dnmt1 and to bind hemimethylated DNA, indicating together with genetic studies a central role in the maintenance of DNA methylation. (cipsm.de)

B cells are therefore particularly dependent on 'quality control' mechanisms to oversee antibody production. (cipsm.de)

Signaling is then transduced across the membrane by altering the configuration of the transmembrane (TM) domain, by dimerization of the TM helical region or a change in the arrangement of the TM helices within such a dimer. (biorxiv.org)

Unlike whole antibodies, they do not show complement system triggered cytotoxicity because they lack an Fc region. (wikipedia.org)

One-half of the domain swapped BTK structure represents the current model for autoinhibited BTK and closely resembles the compact structure of autoinhibited SRC kinases ( Fig. 1b ). (elifesciences.org)
The compact structure of the SH3-SH2-kinase region of BTK is exacted from the domain swapped dimer structure (PDB: 4XI2) solved by Wang et al. (elifesciences.org)
c) Structure of the BTK PHTH domain bound to IP4 (PDB: 1B55) [ 8 ]. (elifesciences.org)
Interestingly, both variants displayed a single dominant structure in the free state. (bvsalud.org)

A particle according to any one of the preceding claims, wherein the protease cleavage site is accessible to the relevant protease (i.e. that which recognises the cleavage site) before the viral particle becomes bound to an eukaryotic cell. (sumobrain.com)
A particle according to any one of claims 1 to 9, wherein the protease cleavage site becomes accessible to die relevant protease only after the viral particle has become bound to an eukaryotic cell. (sumobrain.com)

Those derived from camelid and fish antibodies are less lipophilic and more soluble in water, owing to their complementarity-determining region 3 (CDR3), which forms an extended loop (coloured orange in the ribbon diagram above) covering the lipophilic site that normally binds to a light chain. (wikipedia.org)
A problem with this approach is that the binding region of common IgG consists of two domains (VH and VL), which tend to dimerize or aggregate because of their lipophilicity. (wikipedia.org)
CryoEM reconstructions support conformational heterogeneity in the PHTH-PRR region wherein the globular PHTH domain adopts a range of states arrayed around the autoinhibited SH3-SH2-kinase core. (elifesciences.org)
The monomer is shown for clarity and the TH region bound to Zn 2+ is circled. (elifesciences.org)
A target region within the variable light chain or heavy chain of the first antibody is then identified. (patentpc.com)
Each modified antibody contains at least one variable heavy or variable light-chain in its target region. (patentpc.com)
The DCCM analysis revealed the role of ligand binding in stabilizing TMPK's binding region, as indicated by residual correlation motions. (bvsalud.org)

Induction of binding Abs was measured by ELISA and neutralizing activity by PBMC and TZM-bl assays in all serum and vaginal samples. (europa.eu)
For example, binding can be assessed by immunoassay such as by a radioimmunoassay, enzyme linked immunosorbent assay (ELISA) or electrochemiluminescence assay. (patentpc.com)

Thermal stability assays show that ERKi do not destabilise ERK2 (or ERK1) in vitro, suggesting that ERK2 turnover is a cellular consequence of ERKi binding. (babraham.ac.uk)

We also generated a MUC16 C-terminal domain (MUC16CTD) construct that was stably expressed in MUC16 negative SKOV3 cells. (biomedcentral.com)

The activity to be assessed in the present method can include binding, signal transduction and differentiation, alteration or gene expression, cell proliferation, chemotaxis (cytotoxicity), cancer cell invasion, endothelial proliferation or tube formation. (patentpc.com)

It is important to design antibodies that can be used in therapeutic applications. (patentpc.com)
Multifaceted mutational immunotherapeutic approach to design therapeutic mAbs to combat monkeypox disease via integrated screening algorithms and antibody engineering. (du.ac.in)

MUC16 expression was down-regulated by stably expressing an anti-MUC16 single-chain antibody (scFv) targeted to the endoplasmic reticulum (ER), which prevents cell surface localization of MUC16 in OVCAR3 cells. (biomedcentral.com)
Our data indicated that PET1 inhibits EGFR-mediated cell migration. (biorxiv.org)

Although Sec complexes can form oligomers, it has been suggested that a single copy may serve as an active PCC. (cipsm.de)

Anatomy6

Organisms8

Chemicals and Drugs42

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes46

Information Science4

Health Care1

Monoclonal antibodies2

Kinase domain8

Peptide5

Receptor12

Immunization5

Protein19

Cancer and autoimmune1

Nucleic acids2

Various tumor1

Vaccine4

Cultured in vitro1

Proteins4

Ligands2

Interaction2

Murine2

Nanobodies2

Conformation1

Affinity8

Modulate1

High concentrations1

Intracellular2

Concentrations1

Receptors1

Additionally1

Induce2

Fragment1

Molecule2

Identifies2

Oligomerization5

Residue2

Antigens2

Ability to bind1

Cysteine-rich1

Amino3

Vaccination1

Hinders2

Optimization2

Clones1

Linker2

Humans2

Vaccines1

Specifically1

Parasites1

Light chains2

Mice1

Epitopes1

Targets1

Strongly1

Clinical1

Compounds2

Molecular1

Actin1

Alzheimer's1

Interact1

Mechanisms1

Transmembrane1

Unlike1

Structure4

Viral2

Region7

ELISA2

Cellular1

Terminal1

Differentiation1

Design2

Cell2

Form1