• This study pertains to development of a suitable in vitro model using murine macrophages and Mycobacterium bovis BCG to study proteins expressed during macrophage-myco bacterium interactions. (scirp.org)
  • The results indicate that the present in vitro infection model was able to support the growth of M. bovis BCG in murine macrophages and is an ideal model to determine the pattern of functions of gene expression during the interaction of mycobacterium with macrophages. (scirp.org)
  • 2012). Group B streptococcus GAPDH is released upon cell lysis, associates with bacterial surface, and induces apoptosis in murine macrophages . (up.pt)
  • Immune-stimulated murine macrophages were recently reported to inhibit the growth of Candida albicans (2), Cryptococcus neoformans (9), Histoplasma capsulatum (14, 17), Pneumocystis carinii (11), and Rhizopus spp. (dadospdf.com)
  • We designed a study to evaluate the effect of NO and RNI, generated either chemically or after immunological stimulation of murine macrophages, on the in vitro growth of P. marneffei. (dadospdf.com)
  • SEA and SEB induced dose- and timedependent neutrophil migration into the mouse peritoneal cavity. (ndltd.org)
  • The peritoneal cavity is covered by a serous membrane of mesothelial cells overlying a connective tissue stroma. (dvm360.com)
  • The pressure within the capillaries of the visceral peritoneum is higher than elsewhere, making the peritoneal cavity particularly prone to developing accumulations. (dvm360.com)
  • 1 - 3 Intracranial brain tumors, most commonly medulloblastoma [ Figure 2 ], can metastasize to the peritoneal cavity via ventriculo-peritoneal shunts used to divert excess cerebrospinal fluid. (cytojournal.com)
  • Cytosolic free Ca2+ ([Ca2+]i) homeostasis was investigated in mouse peritoneal macrophages and in the macrophage-like cell line J774. (silverchair.com)
  • P. marneffei conidia were phagocytized by nonstimulated murine J774 macrophages after 2 h of incubation. (dadospdf.com)
  • During the following 24 h, P. marneffei grew as yeast-like cells replicating by fission in the J774 macrophages. (dadospdf.com)
  • The intracellular growth of P. marneffei damaged nonstimulated J774 macrophages, as confirmed by electron microscopy. (dadospdf.com)
  • When J774 cells were stimulated by gamma interferon and lipopolysaccharide, which led to enhanced production of reactive nitrogen intermediates, the percentage of yeast-like cells was significantly reduced and P. marneffei conidia were damaged in the J774 macrophages. (dadospdf.com)
  • Peritoneal macrophages from BALB/c mice were infected with M. bovis BCG and intracellular replication was assessed by { 3 H} thymidine uptake assay which was maximal when macrophage to mycobacterium ratio was 1:10. (scirp.org)
  • The differentially expressed proteins will help in understanding the mycobacterial molecular basis of adaptation to intracellular macrophage environment. (scirp.org)
  • M. tuberculosis is a facultative, intracellular pathogen which resides within the macrophages of the host. (scirp.org)
  • To survive intracellularly it must respond to the intracellular milieu of the macrophages. (scirp.org)
  • Intestinal lamina propria of AIDS patients with concomitant Mycobacterium avium-intracellulare complex (MAC) infection may be packed with PAS-positive granules containing macrophages, but the intracellular bacilli are acid fast. (medscape.com)
  • In the present study we have worked to develop an in vitro model using murine peritoneal macrophages and M. bovis BCG. (scirp.org)
  • We investigated the effect of nitric oxide (NO) and reactive nitrogen intermediates on the in vitro growth of Penicillium marneffei both in a cell-free system and in a novel macrophage culture system. (dadospdf.com)
  • The in vitro model that we developed is the first one to reproduce the transformation of the fungus from a conidial to a yeast-like form, the latter form being found in vivo in infected macrophages. (dadospdf.com)
  • The relationship between monocyte subset and macrophage phenotype is still to be resolved, however tissue macrophages clearly have diverse functions and are key inflammatory mediators [ 23 ]. (biomedcentral.com)
  • This migration was potentiated when the macrophage population was increased by pre-treating the mice with thioglycolate, and was reduced when the peritoneal cavities were washed with sterile saline. (ndltd.org)
  • Malignant mesothelioma is an aggressive neoplasm arising from the surface serosal cells of the pleural, peritoneal, and pericardial cavities. (biomedcentral.com)
  • There were no significant differences in [Ca2+]i between the two macrophage populations whether quin2 or fura-2 were used as Ca2+ indicators. (silverchair.com)
  • It is now recognized that several non-DC myeloid populations express CD11c, including alveolar macrophages and resident (Ly6C low) monocytes and that some macrophage populations express both CD11c and MHC class II [8, 9]. (studybuff.com)
  • We found that reduced kidney function resulted in the expansion of cardiac macrophages, in particular through local proliferation of resident populations. (biomedcentral.com)
  • This review will only include pleural, peritoneal and pericardial fluids. (dvm360.com)
  • Moreover, exogenous supplement of sialic acids can promote plaque progression in aortic arch and aortic sinus being accompanied by the differentiation of macrophages into M1 type in peripheral tissues. (bvsalud.org)
  • SP-B and SP-C work in concert to facilitate rapid adsorption and spreading of DPPC as a monolayer to lower the surface tension at the alveolar air-fluid interface in vivo during expiration, thus preventing atelectasis. (medscape.com)
  • Primary fibroblasts and peritoneal macrophages isolated from Cnn2 knockout mice migrate faster than that of wild type control cells. (wikipedia.org)
  • Deletion of calponin 2 in macrophages also significantly attenuated the development of atherosclerosis lesions in apolipoprotein E knockout (ApoE-/-) mice The expression of calponin 2 is significantly increased in cells cultured on hard versus soft gel substrates that produce high or low traction force and cytoskeleton tension. (wikipedia.org)
  • We assessed cardiac response in two experimental models of CKD and tested macrophage and chemokine implication in monocytopenic CCR2 −/− and anti-CXCL10 treated mice. (biomedcentral.com)
  • The hallmark of the pathologic report was the marked infiltration by foamy macrophages of joints and aortic valves, and prominent deposits of fat within intestinal mucosa and mesenteric lymph nodes, which made Whipple consider this case an obscure disease of fat metabolism and propose the name intestinal lipodystrophy. (medscape.com)
  • You can use an anti-F4/80 to sort murine mature macrophages, associated with anti-BM8 and anti-MOMA-2 it should sort more precisely macrophages from lymphoid tissue. (studybuff.com)
  • He identified periodic acid-Schiff (PAS)-staining granules, most likely representing degenerating bacterial forms, within macrophages isolated from the small bowel as well as other tissue and fluid specimens (eg, pericardium, endocardium, lymph nodes, synovia, lung, brain, meninges) obtained from patients in whom Whipple disease was suspected. (medscape.com)
  • Here, we show that bergenin, a phytochemical isolated from tender leaves of Shorea robusta , activates the MAP kinase and ERK pathways and induces TNF-α, NO and IL-12 production in infected macrophages. (frontiersin.org)
  • The inverse correlation between intramacrophage growth and the amount of nitrite detected in culture supernatants supports the hypothesis that the L-arginine-dependent NO pathway plays an important role in the murine macrophage immune response against P. marneffei. (dadospdf.com)
  • M1 macrophages produce nitric oxide (NO) or reactive oxygen intermediates (ROI) to protect against bacteria and viruses. (studybuff.com)
  • The increased concentration of NO and reactive nitrogen intermediates (RNI) produced by activated macrophages is reported to inhibit the growth of several bacteria, protozoa, fungi, and viruses (4). (dadospdf.com)
  • In this study, we investigated the role of sialic acids in the M1 macrophage polarization and pathogenesis of atherosclerosis. (bvsalud.org)
  • Cortistatin down-regulated the production of inflammatory mediators by endotoxin-activated macrophages. (rupress.org)
  • Macrophages have been shown to be important in all stages of inflammatory and fibrotic kidney disease [ 11 ]. (biomedcentral.com)
  • Furthermore, upon inflammation, both macrophages and pDCs upregulate MHC-II expression. (studybuff.com)
  • It has been well documented that intraphagosomal survival and growth of mycobacteria in macrophage is associated with changes in their gene expression and protein composition upon engulfment [2-4]. (scirp.org)
  • Expression of inducible NO synthase enables macrophages to increase the production of NO from L-arginine and oxygen. (dadospdf.com)
  • BCG, though an attenuated vaccine strain, retains its ability to survive within the macrophages hence expected to mimic some of its behavior with the pathogenic M. tuberculosis inside the macrophages. (scirp.org)
  • Multiplication of the mycobacteria inside the macrophages was ascertained using thymidine uptake assay. (scirp.org)
  • Ca2+]i measurements were performed in both cells in suspension and cells in monolayers loaded with either quin2 or fura-2. (silverchair.com)
  • These cell types can be classified as a) cells that are physiologically under high mechanical tension (e.g., smooth muscle in the wall of hollow organs, epithelial and endothelial cells), b) cells that have high rates of proliferation (e.g., myoblasts), and c) cells that are actively migrating (e.g., fibroblasts and macrophages). (wikipedia.org)
  • Macrophages are one of the most important cells during plaque progression. (bvsalud.org)
  • Enriched monolayer precursor cell cultures from micro-dissected adult mouse dentate gyrus yield functional granule cell-like neurons. (mdc-berlin.de)
  • Macrophages are known to be involved in both chronic kidney disease (CKD) and heart failure, however their role in the development of cardiorenal syndrome is less clear. (biomedcentral.com)
  • We thus sought to investigate the role of macrophages in uremic cardiac disease. (biomedcentral.com)
  • Epithelial ovarian cancer occurs from the malignant transformation of the ovarian surface epithelium and spreads throughout the peritoneal mesothelium [ 2 ]. (biomedcentral.com)
  • These results indicated that the neutrophil recruitment induced by these toxins was dependent on the number of resident macrophages. (ndltd.org)
  • Lung macrophages express CD11c at levels as high as those found on cDCs. (studybuff.com)
  • Seven proteins found to be upregulated during macrophage-mycobacterium interaction were identified by MALDI-TOF. (scirp.org)
  • Resting [Ca2+]i was 110-140 and 85-120 nM for cell suspensions and monolayers, respectively. (silverchair.com)
  • Nitric oxide (NO) has recently been studied as an antimicrobial factor produced by murine and to a lesser extent by human macrophages (16, 20). (dadospdf.com)
  • By incubating macrophages with high concentrations of quin2/AM in the absence of extracellular Ca2+, or by loading EGTA into the cytoplasm, the [Ca2+]i was buffered and clamped to 1-10 nM. (silverchair.com)