Monolayer cell transformation neoplastic. Medical search. Frequent questions

The development of carcinoma requires a complex surrounding microenvironment, which is composed of extracellular matrix (ECM), soluble growth factors, stromal cells and blood vessels [ 1 ]. (oncotarget.com)
Interaction between stromal cells and acute myeloid leukemia (AML) cells in bone marrow (BM) is known to contribute importantly to chemoresistance and disease recurrence. (biomed.news)
Here, we demonstrate that in a niche-like co-culture system, AML cells took up functional mitochondria from bone marrow stromal cells (BMSCs) and inhibition of such mitochondrial transfer by metformin, the most commonly prescribed drug for type 2 diabetes mellitus, significantly enhanced the chemosensitivity of AML cells co-cultured with BMSCs. (biomed.news)
Altogether, this study identifies a potential application of metformin in sensitizing AML cells to chemotherapy and unveils a novel mechanism by which metformin executes such effect via blocking the mitochondrial transfer from stromal cells to AML cells. (biomed.news)
Benassi MS, Picci P, Krenacs T (2015) Prognostic impact of reduced connexin43 expression and gap junction coupling of neoplastic stromal cells in giant cell tumor of bone. (edu.pl)

3T3-L1 cells can be utilized to study a number of cellular and molecular mechanisms related to insulin-resistance, obesity, and diabetes in vitro. (wikipedia.org)
Iron oxide nanoparticle -induced neoplastic-like cell transformation in vitro is reduced with a protective amorphous silica coating. (cdc.gov)
Finally, EPB41L4A-AS2 inhibited breast cancer cell proliferation, migration and invasion and induced cell apoptosis in vitro. (cancerindex.org)
The primary cell cultures, obtained from four human pleural mesotheliomas, were expanded in vitro in a low serum proliferation-permissive medium and the expression of different markers as well as the tumorigenicity in immunodeficient mice was evaluated. (biomedcentral.com)
These cell cultures may provide a valuable in vitro and in vivo model to investigate mesothelioma biology. (biomedcentral.com)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
To this end, in vitro models with cell lines established from human HNSCC have been used, and several chemotherapy sensitivity and resistance assays (CSRA) are currently available. (biomedcentral.com)
For in vitro testing of chemosensitivity, ten established HNSCC cell lines were used. (biomedcentral.com)
Viability assays and cell cycle analyses were used to test in vitro sensitivity to different standard and novel treatments. (por-journal.com)
This study demonstrates in vitro that certain cell types from pleomorphic adenoma are able to clone and survive over a long term and develop subcutaneous tumors in nude mice. (biomedcentral.com)
We showed that functional blockade of CD200 on lymphoma cells or primary CLL cells, both of which express CD200 at high levels, augmented cytotoxic killing of these cells by effector CD8+ T cells in vitro. (ndltd.org)

However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)
Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)
Our results indicated that insulin induces cell proliferation in a dose-dependent fashion in the LNCaP, C4-2, and P69 cell lines. (scienceopen.com)
We also showed that dose-dependent induction of proliferation was connected with changes in the expression of MKI67, CCND1 and CCNE1 genes in well- and poorly differentiated cancer cells. (spandidos-publications.com)
Our study revealed that Ang II influences EC cells in terms of cancer-related processes, and is responsible for increased proliferation, reduction in apoptosis, increased mobility and modulation of adhesion potential. (spandidos-publications.com)
Therefore, various biologically active peptides such as angiotensin, affecting cell proliferation, have become a new area of study in endometrial cancer research. (spandidos-publications.com)
Recently, a number of studies have shown that Ang II plays an important role in proliferation, invasiveness and migration of tumour cells, alteration of expression of cancer-related genes, as well as in physiological tissue remodelling. (spandidos-publications.com)
VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
The time effects of acidic pH e stress were determined with respect to tumor cell proliferation, phenotypic regulation, autophagic control, metabolic plasticity, mitochondrial network dynamics, and metastatic potentials. (biomedcentral.com)
Like other solid tumors, PDAC adopts fermentative glycolysis as an additional metabolic mechanism to compensate for the impaired oxidative phosphorylation to support cell survival and proliferation [ 5 , 6 ]. (biomedcentral.com)
In cocultures, GTPCH expressing fibroblasts stimulated breast cancer cell proliferation and motility, cancer cell Tie2 phosphorylation and consequent downstream pathway activation. (oncotarget.com)
GTPCH interacted with Ang-1 in stromal fibroblasts and enhanced Ang-1 expression and function, which in turn phosphorylated tumor Tie2 and induced cell proliferation. (oncotarget.com)
We have shown that classic Ras proteins promote proliferation and survival, but not migration, in malignant peripheral nerve sheath tumor (MPNST) cells. (biomedcentral.com)
We assessed the expression and activation of these proteins in MPNST cells and inhibited them to determine the effect this had on proliferation, migration, invasion, survival and the phosphoproteome. (biomedcentral.com)
We have previously shown that three related Ras proteins (the classic Ras proteins) are highly activated in malignant peripheral nerve sheath tumor (MPNST) cells with neurofibromin loss and that they drive cancer cell proliferation and survival by activating multiple cellular signaling pathways. (biomedcentral.com)
Cell proliferation was decided using MTT assay, while apoptosis was analyzed with circulation cytometry using Annexin V-fluorescein isothiocyanate/propidium iodide assay. (forgetmenotinitiative.org)
or gene silencing significantly inhibited cell proliferation and induced apoptosis in RPMI 8226 cells ( (forgetmenotinitiative.org)

Abrogation of TGF beta signaling in mammary carcinomas recruits Gr-1+CD11b+ myeloid cells that promote metastasis. (academicinfluence.com)
All UM-SCC-lines (University of Michigan Squamous Cell Carcinomas) were kindly provided by Dr. T. Carey, University of Michigan, Ann Arbor, USA. (biomedcentral.com)
In our previous study, we proposed a concept of focal carcinomas in pleomorphic adenoma which is an advanced stage of accumulated atypical cells with P53 over-expressions as an initial stage or a latent form of apparent carcinomas secondarily arising in pleomorphic adenoma [ 3 ]. (biomedcentral.com)
The malignant tumours consisted of 18 adenoid cystic adenomas, 10 mucoepidermoid carcinomas, 5 acinic cell carcinomas, 5 carcinoma in pleomorphic adenoma as well as of 12 other malignancies belonging to 7 different tumour entities. (researchgate.net)
Abnormal DNA content has been related to aggressive behavior in Mucoepidermoid Carcinomas (MEC), Adenoid Cystic Carcinomas (AdCC), Acinic Cell Carcinomas (ACC) and oncocytomas (Onc) [8]. (researchgate.net)
various biopsies from different european malignant lymphomas, two biopsies from nasopharyngeal carcinomas, and material from non-neoplastic lymph nodes were assayed for the presence of epstein-barr virus (ebv) dna by nucleic acid hybridization. (liverpool.ac.uk)

Inhibition of cell growth by seven chemotherapeutic agents (cisplatin, carboplatin, 5- fluorouracil, methotrexate, bleomycin, vincristin, and paclitaxel) was measured using metabolic MTT-uptake assay and correlated to clinically-achievable plasma concentrations. (biomedcentral.com)
PF338 tumor cells were resistant to MEK- and TGF-β signaling-inhibition but sensitive to PIK3CA- and PARP-inhibition and first-line chemotherapeutics. (por-journal.com)
Strikingly, histone deacetylase (HDAC) inhibition markedly reduced cell viability by inducing a dose-dependent G0/1 arrest and led to mesenchymal-epithelial transition as evidenced by morphological change and increased E-cadherin and β-catenin expression. (por-journal.com)
Our data suggest that HDAC inhibition is effective in a novel UCS cell line by interfering with both viability and differentiation. (por-journal.com)
Curcumin reduces fascin expression through JAK/STAT3 pathway inhibition, which interferes with the cellular interactions essential for the metastasis and recurrence of ovarian cancer cells. (biomedcentral.com)
The inhibition of NAMPT is usually shown to induce cell death and reduce osteoclastogenesis in MM [10,11]. (forgetmenotinitiative.org)

METHODS: Whole-cell patch-clamp experiments were performed in human embryonic kidney (HEK 293) cells and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) together with immunofluorescent confocal and high resolution microscopy, auto-ubiquitinylation assays, and co-immunoprecipitation experiments to test the functional interactions between hERG and RNF207. (bvsalud.org)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)
Biological assays and gene expression analysis were performed on three cell lines: ISH, MFE-296 and MFE-280. (spandidos-publications.com)
Biological assays were performed on well-[Ishikawa (ISH)] moderately (MFE-296) and poorly (MFE-280) differentiated human adenocarcinoma cancer cell lines, representing the G1, G2 and G3 stage of EC. (spandidos-publications.com)
MTS assays showed that cell viability was different at various concentration of curcumin, and as concentration increased, cell viability tended to decrease. (biomedcentral.com)
Based on this method, we performed cytotoxicity assays on 42 AsPCs grown both as monolayers and spheroids. (biomedcentral.com)

Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)
Here, we analyze the effect of HHV-8 infection on HIV replication in monocyte-macrophage and endothelial cells, as potential targets of coinfection. (ashpublications.org)
Primary or transformed monocytic and endothelial cells were infected with a cell-free HHV-8 inoculum and subsequently infected with lymphotropic or monocytotropic strains of HIV. (ashpublications.org)
The results suggest that HHV-8 might be a cofactor for HIV progression and that HHV-8-infected endothelial cells might play a relevant role in transendothelial HIV spread. (ashpublications.org)
Baringer SL , Lukacher AS , Palsa K , Kim H , Lippmann ES , Spiegelman VS , Simpson IA , Connor JR , Amyloid-β exposed astrocytes induce iron transport from endothelial cells at the blood-brain barrier by altering the ratio of apo- and holo-transferrin Journal of neurochemistry. (vanderbilt.edu)

The establishment, characterization, and tumorigenicity of a new epithelial cell line (PC-3) from a human prostatic adenocarcinoma metastatic to bone is reported. (scienceopen.com)
Single - cell RNA-sequencing analysis revealed that CREB3L1 expression gradually increased during the neoplastic progression of a thyroid follicular epithelial cell to an ATC cell, accompanied by the activation of the extracellular matrix (ECM) signaling. (biomedcentral.com)
Childress KO , Cencer CS , Tyska MJ , Lacy DB , Nectin-3 and shed forms of CSPG4 can serve as epithelial cell receptors for TcdB mBio. (vanderbilt.edu)

Conclusions Bcl2 , Myc and Ccnd1 or Bcl2 , Myc and CCND3 synergistically transformed mouse primary B cells into aggressive malignant cells. (haematologica.org)
When malignant cells metastasize to distant sites in the body, morbidity and mortality increase significantly (3) . (aacrjournals.org)
At the cellular level, malignant cells have overcome restraints on cell growth and migration that result from physical linkages and signals conveyed by cell-cell contacts (5) . (aacrjournals.org)
Malignant cells often have increased interactions with surrounding ECM 3 proteins, which provide linkages and signals that promote several aspects of metastasis (6) . (aacrjournals.org)
Finally, IR isoforms form heterodimers, IR-A/IR-B, and hybrid IR/IGF-IR receptors (HR-A and HR-B). The functional characteristics of such hybrid receptors and their role in physiology, in diabetes, and in malignant cells are not yet fully understood. (scienceopen.com)
As malignant cells have a tendency to round up in body fluids these non-epithelial neoplasms can therefore mimic reactive mesothelial cells and metastatic adenocarcinoma. (cytojournal.com)

The cultured cells show anchorage-independent growth in both monolayers and in soft agar suspension and produce subcutaneous tumors in nude mice. (scienceopen.com)
clonal transformation of human leukocytes by epstein-barr virus in soft agar. (liverpool.ac.uk)

3 - 7 The synergistic effect of BCL2 and MYC has also been implicated in the histological and clinical transformation of indolent follicular lymphoma into a more aggressive lymphoma. (haematologica.org)
The inflammatory AML should be distinguished from other tumors with inflammatory background such as inflammatory myofibroblastic tumor and follicular dendritic cell tumor and deserves wider recognition for its occurrence as a primary hepatic tumor. (biomedcentral.com)
Of these, inflammatory or pelioid pattern usually presents as a focal finding within the tumor, but very rarely, they become the predominant pattern [ 4 ], creating great diagnostic confusion with other tumors such as inflammatory myofibroblastic tumor (IMT), follicular dendritc cell (FDC) tumor and other hepatic mesenchymal neoplasms. (biomedcentral.com)

Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)
These results demonstrate a functional role for PMS2 to protect against PCa progression by enhancing apoptosis of PCa cells. (oncotarget.com)

Importantly, UCS is the prototype tumor for epithelial-mesenchymal transition (EMT), a reversible biological process that associates with tumor progression and metastasis and in which epithelial cells transform into more invasive mesenchymal cells by losing their epithelial properties [ 10 - 12 ]. (por-journal.com)
After CREB3L1 knockdown, the presence of CAFs inhibited the growth of ATC spheroids and the metastasis of ATC cells. (biomedcentral.com)

These cells were originally used to study the properties of transformed cells and the mechanisms of neoplastic transformation. (wikipedia.org)
In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and the underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). (cdc.gov)
The aims of this study were to investigate whether insulin can directly induce mitogenic activities in PCa-derived cell lines and to examine the mechanisms responsible for these actions. (scienceopen.com)
This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)
Hence, a better understanding of the impact of interstitial acidity and how tumor cells coordinate various adaptation mechanisms to cope with the acidified microenvironment is critical to improving therapeutic efficacy against solid cancers especially lethal PDAC. (biomedcentral.com)

The 3T3 process began with cultures having 3 days to propagate on a plate (the first "3"), and then a transfer (the "T") of 300,000 cells (second "3") to a new plate to restart the process. (wikipedia.org)
Our work was aimed to establish from fresh human pleural mesothelioma samples cell cultures maintaining tumorigenic properties. (biomedcentral.com)
The established mesothelioma cell cultures are able to engraft, after pseudo orthotopic intraperitoneal transplantation, in immunodeficient mouse and maintain this ability to after serial transplantation. (biomedcentral.com)
Our cell cultures were strongly positive for CD46, CD47, CD56 and CD63 and were also strongly positive for some markers never described before in mesothelioma cell lines, including CD55, CD90 and CD99. (biomedcentral.com)
We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
Three-dimensional cultures were performed using several oral cancer cell lines. (medsci.org)
We have developed a 3D (spheroid) functional assay to assess the sensitivity of two PARPis (niraparib and olaparib) in ascites-derived primary cell cultures (AsPCs) from HGSOC patients. (biomedcentral.com)

In 1971, Green and Kehinde established a subline of 3T3 cells, called 3T3-L1, which demonstrated a greater propensity for differentiation into adipocytes and a higher affinity for lipid deposition. (wikipedia.org)
The process of adipogenesis, and the start of differentiation can be induced in 3T3-L1 cells through the addition of compounds such as a synthetic glucocorticoid, a phosphodiesterase inhibitor, and insulin. (wikipedia.org)
Since 3T3-L1 cells are an immortalized cell line and require a hormonal differentiation, there has been much debate on their comparability to in vivo studies and primary cell lines. (wikipedia.org)
Studies suggest that differentiation efficiency can rely on factors such as culture dish material, culture dish provider, culture dish type, cell confluence at the time of differentiation. (wikipedia.org)
Its effect and effectiveness appear to be highly connected with the differentiation status of the cancerous cells, as Ang II appears to play a crucial role in the early and late stages of malignant transformation. (spandidos-publications.com)
SM-AP1-3 cells were immunopositive for keratin only, indicating their duct-epithelial or squamous cell differentiation, while SM-AP4/5 cells were positive for both keratin and S-100 protein, indicating their myoepithelial cell differentiation. (biomedcentral.com)
We subsequently discuss how β-TCP can regulate osteogenic processes to aid bone repair/healing, namely osteogenic differentiation of mesenchymal stem cells, formation of blood vessels, release of angiogenic growth factors, and blood clot formation. (frontiersin.org)
Differentiation and integration of grafted cells in the host tissue were validated with immunofluorescence staining using human-specific antibodies. (biomedcentral.com)

Here we report increased levels of the EphA2 (ECK) protein tyrosine kinase in clinical specimens and cell models of breast cancer. (aacrjournals.org)
In the 1980s, new devices were results of monolayer cytology specimens were reviewed developed for enhancing the collection of exfoliated cells by quality control pathologists, only negative and low- from the cervix. (finder-articles.com)
The Sumner-Lab uses analytical methods to detect tens of thousands of signals for molecules that are present in biological specimens (such as urine, serum, plasma, feces, sweat, tissues, and cells). (uncnri.org)

Basal cell culture medias for 3T3-L1 cells tends to contain a version of Dubelcco's Modified Eagle's Medium (DMEM), fetal bovine serum (FBS) , and an antibiotic at specific levels dependent upon protocol. (wikipedia.org)
3T3-L1 cells are commonly utilized as an effective model for white adipocytes because of their similar fibroblast-like morphology, lipid accumulation, gene expression, and basal energy exchanges and transformations. (wikipedia.org)
The group of benign tumours included 164 pleomorphic adenomas, 51 Warthin's tumours, 7 basal cell adenomas, 2 lipomas as well as 5 other different tumours. (researchgate.net)
In breast tissue, the luminal epithelial cells express CK 8/18, CK 7 and CK 19, while basal/myoepithelial cells express CK 5/6, CK 14 and CK 17. (ijpmonline.org)

Aside from its usages, this cell line is widely developed and can be purchased for continuous propagation for numerous research studies. (wikipedia.org)
A method for AsPCs preparation was established based on a matrix (agarose), allowing for easy isolation and successive propagation of monolayer and 3D AsPCs. (biomedcentral.com)
Cell culture is the most popular method of virus propagation because of its high sensitivity. (ijpmonline.org)

Transplanted cells in nude mice formed subcutaneous tumors, which had histological features of squamous cell carcinoma with apparent keratinizing tendencies. (biomedcentral.com)
The histological features of squamous cell carcinoma from the transplanted cell systems in nude mice might suggest a secondary onset of malignancy from a pre-existing benign adenoma. (biomedcentral.com)
In three cases which initially were taken for pleomorphic adenomas by routine histological examination, aneuploid cell populations exposed by DNA flow cytometric analysis gave rise to a closer inspection of the suspect lesions. (researchgate.net)

Establishment and characterization of a human prostatic carcinoma cell line (PC-3). (scienceopen.com)

Systemic treatment of head and neck squamous cell carcinoma (HNSCC) includes a variety of antineoplastic drugs. (biomedcentral.com)
Other studies in oral cancer [23,24] reported that there is no relationship between the histopathological grade of oral epithelial dysplasia or oral squamous cell carcinoma and the ploidy status. (researchgate.net)

Although there have been many reports regarding the anticancer properties of curcumin, its inhibitory effects on migration and invasion of ovarian cancer cells, particularly in the context of fascin expression, have not been reported. (biomedcentral.com)
The attachment assay, migration assay and invasion assay of the ovarian cancer cells exhibited a statistically significant decrease. (biomedcentral.com)

Metastatic cells have acquired the abilities to break away from the primary tumor, translocate to distant sites in the body, and colonize a foreign microenvironment (4) . (aacrjournals.org)
While most tumors metastatic to the serous membranes are of epithelial origin, cytologists should be aware that non-epithelial neoplasms can also cause malignant effusions including sarcomas, melanomas, germ cell tumors, and, more rarely, brain tumors. (cytojournal.com)
Whereas immunohistochemistry of the metastatic tumor showed a predominantly sarcomatous vimentin positive tumor that has lost E-cadherin expression, PF338 cells demonstrated biphasic growth and carried mutations in KRAS , PIK3CA , PTEN and ARID1A . (por-journal.com)
Hapach LA , Wang W , Schwager SC , Pokhriyal D , Fabiano ED , Reinhart-King CA , Phenotypically sorted highly and weakly migratory triple negative breast cancer cells exhibit migratory and metastatic commensalism Breast cancer research : BCR. (vanderbilt.edu)

RESULTS: We demonstrated that chemotherapy-stimulated CAFs promoted tumorigenic and stem cell-like properties of NSCLC cells, which contribute to their chemoresistance. (cdc.gov)
We also show that EphA2 overexpression is sufficient to confer malignant transformation and tumorigenic potential on nontransformed (MCF-10A) mammary epithelial cells. (aacrjournals.org)
However all the cell lines established so far were grown in medium containing at least 10% serum, and it has been shown that primary cell lines cultured under these conditions lose their ability to differentiate, acquire gene expression profiles that differ from that of tissue specific stem cells or the primary tumor they derive from, and in some cases are neither clonogenic nor tumorigenic. (biomedcentral.com)

TGF-beta and immune cells: an important regulatory axis in the tumor microenvironment and progression. (academicinfluence.com)
A novel UCS cell line (PF338) was established from the malignant pleural effusion of a 59-year-old patient at time of disease progression. (por-journal.com)
In a primary tumor mass, the ECM is strictly modulated in a tumor - permissive way, which in turn facilitates tumor progression and influences cancer cell invasion. (biomedcentral.com)
The cells were analyzed by circulation cytometry for monitor cell cycle progression. (irjs.info)

Our results offer potential target pathways and genes for further development of the secretory power in mammalian cell factories. (bvsalud.org)
Materials and Methods Mammalian cell tradition and harvest HeLa, HEK 293T and A549 cells [13, 14] were cultured in Dulbeccos Modified Eagles Medium (pH7.4) with 10% (v/v) Fetal Bovine Serum at 37C. (irjs.info)
Here, we employed a genetically encoded high-affinity H2 O2 sensor, HyPer7, in mammalian tissue culture cells to investigate different modes of mitochondrial H2 O2 release. (biomed.news)

Design and Methods Growth in culture, colony formation and oncogenicity in vivo were assessed in mouse primary B cells exogenously expressing various combinations of Bcl2 , Myc and Ccnd1 . (haematologica.org)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
Cancer research has been conducted using cultured cells as part of drug discovery testing, but conventional two-dimensional culture methods are unable to reflect the complex tumor microenvironment. (medsci.org)
Methods: Fixed number of Vero and Hep2 cells were preserved at -85ºC deep freezer in separate vials and were revived at one month interval to check the growth pattern. (ijpmonline.org)
Our results offer a even more unique understanding of the jobs of and genes in the molecular pathogenesis of MM. MATERIALS AND METHODS Cell collection The myeloma cell collection RPMI 8226 was purchased from the American Type Culture Collection (ATCC, USA). (forgetmenotinitiative.org)

The fascin was quantified using cell viability test and Western blot. (biomedcentral.com)
The main challenge for large bone defect repair and regeneration remains the inadequate recruitment of mesenchymal stem cells (MSCs), reduced vascularization, and decreased growth factors stimulation within the scaffold construct to support cell viability and tissue growth. (frontiersin.org)
Abstract The candida Ipi3p is required for DNA replication and cell viability in Ipi1-3p have been found to be required for cell viability and for processing of the ITS2 sequences from 35S pre-rRNA in pre-60S ribosomal particles [8, 9]. (irjs.info)

Pancreatic ductal adenocarcinoma (PDAC), often simply described as pancreatic cancer, is the most prevalent neoplastic disease of the pancreas [ 1 ]. (biomedcentral.com)
Bechyne I, Szpak K, Madeja Z, Czyz J (2011) Functional heterogeneity of non-small lung adenocarcinoma cell sub-populations. (edu.pl)

Finally, the presence of RNF207G603fs with hERGT613M results in significantly prolonged action potential durations and reduced hERG current in human-induced pluripotent stem cell-derived cardiomyocytes. (bvsalud.org)
CONCLUSION: Our results show that PDL-1-positive CAFs modulate stem cell-like properties of NSCLC cells by secreting elevated HGF, thereby promoting chemoresistance. (cdc.gov)
Results Primary B cells exogenously expressing Bcl2 , Myc and Ccnd1 showed factor-independent growth ability, enhanced colony-forming capability and aggressive oncogenicity, unlike the cases observed with the expression of any combination of only two of the genes. (haematologica.org)
These results demonstrate that CREB3L1 maintains the CAF - like property of ATC cells by activating the ECM signaling, which remodels the tumor stromal microenvironment and drives the malignancy of ATC. (biomedcentral.com)
B) qRT-PCR results display the mRNA level of hIPI3 fluctuated during the cell cycle and the highest level was between M phase and early G1 phase. (irjs.info)
Previous results indicate that the HHV-8 viral transactivator ORF50 interacts synergistically with Tat protein in the transactivation of human immunodeficiency virus (HIV) long terminal repeat (LTR), leading to increased cell susceptibility to HIV infection. (ashpublications.org)
The results show that HHV-8 coinfection markedly increases HIV replication in both cell types. (ashpublications.org)
Dynamic adaptation of cytosolic thioredoxin reductase levels during metabolic changes results in improved H2 O2 handling and explains previously observed differences between cell types. (biomed.news)

Cells cultured as 3D were embedded as tissue blocks. (frontiersin.org)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)
H-E staining showed tumor tissue-like structures specific to each cell line. (medsci.org)
In UCS, several studies identified characteristic EMT-related expression signatures including active TGF-β signaling in tissue and cell lines [ 7 , 11 , 13 , 14 ]. (por-journal.com)
We first asked whether a cell-autonomous mechanism could contribute to exhaled biomarkers, so we began by searching for thematic differences in metabolic gene expression between EAC and relevant control tissue in archived transcriptomic datasets. (nature.com)
In this review, we introduce the broad application of β-TCP in tissue engineering and discuss the different approaches that β-TCP scaffolds are customized, including physical modification (e.g., pore size, porosity and roughness) and the incorporation of metal ions, other materials (e.g., bioactive glass) and stem cells (e.g., mesenchymal stem cells). (frontiersin.org)
Angiomyolipoma (AML) is a rare mesenchymal neoplasm of the tumor, composed of a varying heterogeneous mixture of three tissue components: blood vessels, smooth muscle and adipose cells. (biomedcentral.com)
Histologically similar to those in the kidney, hepatic AML consists of a mixture of myoid cells, adipose tissue and thick-walled vessels. (biomedcentral.com)
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
This may be due to late diagnosis of these tumors in our patients as most cases diagnosed at advanced stage and the sections were taken from deep tumor tissue to increase the number of tumor cells in our samples. (researchgate.net)
Cell Tissue Res 360: 685-699. (edu.pl)

It was reported that a human being protein comprising five WD40 repeat domains can stabilize ORC binding to chromatin by interacting with ORC and modulating the chromatin association of ORC in human being cells [12], suggesting that WD40 repeat proteins play important roles in human being DNA replication. (irjs.info)
Proteins in cells with or without DN R-Ras expression were differentially labeled with SILAC and mass spectrometry was used to identify phosphoproteins and determine their relative quantities in the presence and absence of DN R-Ras. (biomedcentral.com)
Both proteins were activated in neurofibromin-null MPNST cells. (biomedcentral.com)
Here, we examined the expression, activation and action of R-Ras proteins in MPNST cells that have lost neurofibromin. (biomedcentral.com)
Willet AH , Chen JS , Ren L , Gould KL , Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins Molecular biology of the cell. (vanderbilt.edu)

3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. (wikipedia.org)
The 3T3-L1 cell line is a sub clone that was initially developed from a mouse embryo, from a clonal expansion of Swiss 3T3 cells. (wikipedia.org)
Single-cell genetic analysis of clonal dynamics in colorectal adenomas indicates CDX2 gain as a predictor of recurrence. (cancerindex.org)

Western blotting was used for protein expression analysis of the collected cell masses (spheroids), and H-E staining was used for structural observation. (medsci.org)
Spheroids made from oral cancer cell lines appeared to have tumor-like characteristics that may reflect their clinical significance. (medsci.org)

The identification of new mesothelioma markers may be useful for diagnosis and/or prognosis of this neoplasia as well as for isolation of mesothelioma tumor initiating cells. (biomedcentral.com)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)

To identify tyrosine kinases that control tumor cell adhesion, we developed novel technologies to generate monoclonal antibodies against tyrosine kinases in cancer cells (8) . (aacrjournals.org)
Each cell line formed spheroidal cell aggregates and showed enhancement of cell adhesion molecules over time. (medsci.org)
Cisplatin showed concentration-dependent antitumor effects due to loss of cell adhesion and spheroid disruption in each cell line, while cetuximab exhibited antitumor effects that correlated with EGFR expression in each cell line. (medsci.org)

It is still poorly understood how and which benign pleomorphic adenoma cells transform into its malignant form, carcinoma ex pleomorphic adenoma. (biomedcentral.com)
We have established five cell systems from a benign pleomorphic adenoma of the parotid gland of a 61-year-old woman. (biomedcentral.com)
We established and characterized five cell systems (designated as SM-AP1 to SM-AP5) from a benign pleomorphic adenoma of the parotid gland. (biomedcentral.com)
In addition, they had ductal arrangements or plasmacytoid appearances of tumor cells and myxoid or hyaline stromata, indicating some characteristics of pleomorphic adenoma. (biomedcentral.com)
Examination of consecutive slides actually revealed small assemblies of carcinoma cells that required a final diagnosis as non-invasive carcinoma in pleomorphic adenoma. (researchgate.net)

The response to PARPis treatment in monolayer AsPCs, was significantly higher, compared to 3D AsPCs, as 88% and 52% of the monolayer AsPCs displayed sensitivity to niraparib and olaparib respectively, while 66% of the 3D AsPCs were sensitive to niraparib and 38% to olaparib, the latter being more consistent with previous estimates of HRD (40%-60%) in EOC. (biomedcentral.com)

3T3-L1 cells of the adipocyte morphology increase the synthesis and accumulation of triglycerides and acquire the signet ring appearance of adipose cells. (wikipedia.org)

Due to the loss of CREB3L1, ATC cells were unable to activate alpha - smooth muscle actin (α - SMA) - positive cancer - associated fibroblasts (CAFs). (biomedcentral.com)
In coimplantation xenografts, GTPCH in fibroblasts enhanced tumor growth, upregulating Ang-1 and alpha-smooth muscle actin mainly in fibroblast-like cells. (oncotarget.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

These cells will be identified as 3T3 only after they are taken through 20 to 30 passages and have established at a stable growth rate. (wikipedia.org)
During this period, cells can undergo hyperplastic growth until they are differentiated. (wikipedia.org)
Our aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth of these tumors. (frontiersin.org)
Interestingly, stimulation of EphA2 reverses the malignant growth and invasiveness of EphA2-transformed cells. (aacrjournals.org)
Recent studies have identified overexpression of HER2 or epidermal growth factor receptor in some tumors and used this knowledge to develop successful approaches for therapeutic targeting of cancer cells (2) . (aacrjournals.org)
Growth inhibitor from BSC-1 cells closely related to platelet type beta transforming growth factor. (academicinfluence.com)
Transforming growth factor production by chemically transformed cells. (academicinfluence.com)
All drugs inhibited cell growth in a concentration-dependent manner with an IC50 comparable to that achievable in vivo . (biomedcentral.com)
In prostate, the functional role of this gene has never been reported and in this study, our aim was to investigate the effect of PMS2 on growth properties of prostate cancer (PCa) cells. (oncotarget.com)
High-affinity zinc uptake system protein mutant ( znuA mutant) showed reduced growth in zinc chelated medium, and failed to replicate in HeLa cells and mouse bone marrow-derived macrophages. (go.jp)
Transformation of znuA mutant with a shuttle vector pBBR1MCS-4 containing znuA gene restored the growth in zinc chelated medium and intracellular replication in HeLa cells and macrophages to a level comparable to that of wild-type strain. (go.jp)
According to the predominant component, growth pattern, cell type, and other features, the tumors were subcategorized into trabecular, pelioid and inflammatory variants. (biomedcentral.com)
GTPCH/Ang-1 interaction in stromal fibroblasts and activation of Tie2 on breast tumor cells could play an important role in supporting breast cancer growth. (oncotarget.com)
In this category of experimentation, besides the use of locally delivered trophic factors (such as brain-derived neurotrophic factor (BDNF)-, glial cell line-derived neurotrophic factor (GDNF)-, and fibroblast growth factor (FGF)-peptides or growth factors-gene-encoding vectors) [ 8 ], regionally grafted fetal or embryonic stem cell-derived neuronal precursors are frequently used [ 9 - 18 ]. (biomedcentral.com)

Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)
Enrichment of cancer stem cells was observed in neurospheres by FACS using CD133 antibody and RT-PCR. (frontiersin.org)

The objective of this study was to identify additional cooperative gene(s) associated with BCL2 and MYC or MYC and CCND1 in human B-cell lymphomagenesis. (haematologica.org)
Previous studies have shown PMS2 to be deficient in DU145 cells and this lack of expression was confirmed by Western blotting whereas normal prostatic PWR-1E and RWPE-1 cells expressed this gene. (oncotarget.com)
In UCS, TP53 has been identified as the most frequently mutated gene, followed by mutations in the PI3K pathway, KRAS , cell cycle regulators including FBXW7 and chromatin remodeling and histone genes including ARID1A [ 7 - 9 ]. (por-journal.com)
They were characterized by immunofluorescence, classical cytogenetics, p53 gene mutational analysis, fluorescence in-situ hybridization, and histopathological and immunohistochemical examinations of their xenografts, to demonstrate their potency of secondary transformation. (biomedcentral.com)
Our study exhibited that and play pivotal functions in the molecular pathogenesis of MM. This is usually the first statement describing the possible functions of in myelomagenesis and its potential role as a SU 11654 therapeutic target in MM. gene promotes tumorigenesis through constant NAD resynthesis to provide adequate energy for rapidly proliferating malignancy cells [9]. (forgetmenotinitiative.org)
Besides CHS, mutation in gene is usually one of the important factors that trigger hemophagocytic lymphohistiocytosis, a insufficiency in resistant program function, and life-threatening disease characterized by uncontrolled macrophage and T-cell account activation [14]. (forgetmenotinitiative.org)
No.: SR300809, USA) were used to silence the respective gene in RPMI 8226 cells. (forgetmenotinitiative.org)

CREB3L1 knockdown dramatically attenuated invasion of ATC cells, whereas overexpression of CREB3L1 facilitated the invasion of papillary thyroid carcinoma (PTC) cells. (biomedcentral.com)

The elevated glucose consumption-coupled with increased excretion of lactate and protons from glycolytic cells to the poorly perfused extracellular spaces-leads to an acidification of the tumor microenvironment [ 7 , 8 ]. (biomedcentral.com)
Although still not fully understood, an acidic microenvironment with an inverted pH gradient of extracellular pH (pH e ) lower than intracellular pH (pH i ) appears to impose selective pressures whereby tumor cells must adapt or die [ 10 , 11 ]. (biomedcentral.com)
Therefore, disruption of a crosstalk between AML cells and BM microenvironment may offer a promising therapeutic strategy for AML treatment. (biomed.news)

Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
Cellular stress responses are also regulated through the activity of unpaired connexons (hemichannels) and via the intracellular interference of connexins with the cell cycle and pro-apoptotic machinery. (edu.pl)
Young KM , Reinhart-King CA , Cellular mechanosignaling for sensing and transducing matrix rigidity Current opinion in cell biology. (vanderbilt.edu)

Silencing PDL-1 expression suppressed CAFs' ability to promote stem cell-like properties and invasiveness of lung cancer cells, favoring chemoresistance. (cdc.gov)
A spontaneous cell line-like cell culture with high percentage of stem cells has been established from a DIG tumor for the first time. (frontiersin.org)
Intraspinal grafting of human neural stem cells represents a promising approach to promote recovery of function after spinal trauma. (biomedcentral.com)
Here we characterized the effect of intraspinal grafting of clinical grade human fetal spinal cord-derived neural stem cells (HSSC) on the recovery of neurological function in a rat model of acute lumbar (L3) compression injury. (biomedcentral.com)

In vivo experiments were conducted using a co-implantation xenograft mouse model with live cell imaging and immunohistochemistry. (cdc.gov)
HHV-8 infection induces also HIV reactivation in chronically infected cell lines and in peripheral blood mononuclear cells (PBMCs) from patients with asymptomatic HIV, suggesting the possibility that similar interactions might take place also in vivo. (ashpublications.org)
In addition, metformin potentiated the antitumor efficacy of cytarabine (Ara-C) in vivo in an NCG immunodeficient mouse xenograft model by inhibiting the mitochondrial transfer and OXPHOS activity in the engrafted human AML cells. (biomed.news)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

establishment of ebna-expressing cell lines by infection of epstein-barr virus (ebv)-genome-negative human lymphoma cells with different ebv strains. (liverpool.ac.uk)

However, most previous studies have only reported the fate of cancerous cells upon cursory exposure to acidic pH e conditions. (biomedcentral.com)

Impacts of organomodified nanoclays and their incinerated byproducts on bronchial cell monolayer integrity. (cdc.gov)
DNA mismatch repair (MMR) enzymes act as proofreading complexes that maintains genomic integrity and MMR-deficient cells show an increased mutation rate. (oncotarget.com)

letters to the editor: bone marrow transplantation and leukemic transformation of transplanted cells]. (liverpool.ac.uk)

Transcriptomic and functional analyses indicate significantly higher metabolism and oxidative phosphorylation in EPO producers compared with parental and GFP cells. (bvsalud.org)
Moreover, niraparib displayed a significantly stronger cytotoxic effect in both in 3D and monolayer AsPCs, which was confirmed by consecutive analyses of the HR pathway activity (γH2AX foci formation) in PARPis-sensitive and resistant AsPCs. (biomedcentral.com)

3T3-L1 cells have a fibroblast-like morphology, but, under appropriate conditions, the cells differentiate into an adipocyte-like phenotype, providing an exemplar model for white adipocytes. (wikipedia.org)
The cells were cultured in a serum-containing medium and demonstrated a fibroblast-like morphology. (wikipedia.org)
However, the clinical significance of GTPCH expression in breast cancer is still elusive and how GTPCH regulates stromal fibroblast and tumor cell communication remains unknown. (oncotarget.com)

CREB3L1 knockdown significantly decreased the expression of collagen subtypes in ATC cells and the fibrillar collagen in xenografts. (biomedcentral.com)

These receptors seem to enhance cell responsiveness to IGFs. (scienceopen.com)

In conclusion, insulin exhibits direct mitogenic activities in PCa cells, which are mediated exclusively through the INSR. (scienceopen.com)

Our previous studies revealed that the levels of protein tyrosine phosphorylation regulate a balance between cell-cell and cell-ECM adhesions in epithelial cells (7) . (aacrjournals.org)
The chemo-sensitizing effect of metformin was acted through reducing the mitochondrial transfer and mitochondrial oxidative phosphorylation (OXPHOS) in the recipient AML cells. (biomed.news)
Oxidative phosphorylation (OXPHOS) takes place in mitochondria and is the process whereby cells use carbon fuels and oxygen to generate ATP. (biomed.news)

In the early 1980s, it was discovered that these cells could be induced to differentiate into adipocytes in response to hormonal stimulation. (wikipedia.org)

To this end, we used several PCa-derived cell lines, representing early and advanced stages of the disease. (scienceopen.com)
In this regard, tumor cell lines development has been an important tool to study the biological properties of many tumors. (biomedcentral.com)
By real time PCR we found that our cell lines expressed high mRNA levels of typical mesothelioma markers as mesothelin (MSLN) and calretinin (CALB2), and of BMI-1, a stemness marker, and DKK1, a potent Wingless [WNT] inhibitor. (biomedcentral.com)
Ten human cell lines were obtained from HNSCC, including one with experimentally-induced cisplatin resistance. (biomedcentral.com)
Chemosensitivity of HNSCC cell lines can be determined using the MTT-uptake assay. (biomedcentral.com)
For this purpose, ten HNSCC cell lines were tested for chemosensitivity against seven cytostatic drugs commonly used in the clinic, namely CIS, CPT, 5-FU, methotrexate (MTX), bleomycin (BLM), vincristine (VCR), and paclitaxel (PTX). (biomedcentral.com)
cells of two ebna (epstein-barr virus nuclear antigen)-negative human lymphoma cell lines, bjab and ramos, were infected with two strains of epstein-barr virus (ebv). (liverpool.ac.uk)
However, the need of high cost liquid nitrogen for storage of cell lines is one of the main limiting factor for its widespread use in developing countries. (ijpmonline.org)
The present study was therefore carried out to standardize the preservation of continuous cell lines at deep freezer (-85ºC) for 6 months. (ijpmonline.org)

Although pathologists in their daily services of surgical pathology had recognized such singular atypical cells in pleomorphic adenomas, these atypical cells were not always regarded as evidence or the source for malignant transformation [ 4 - 6 ]. (biomedcentral.com)

Since the establishment of the first cell line by Gey et al. (intechopen.com)

For the functional screening, Bcl2 - and Myc- expressing primary B cells were infected with a retroviral cDNA library. (haematologica.org)

Formerly OXPHOS was thought to be reduced in tumours and that glycolysis was the critical pathway for generation of ATP but it is now clear that OXPHOS, at least in many tumour types, plays a critical role in delivering the bioenergetic and macromolecular anabolic requirements of cancer cells. (biomed.news)

Bacterial internalization into HeLa cells and macrophages and co-localization with either late endosomes or lysosomes of znuA mutant were not different from those of wild-type strain. (go.jp)
A) HeLa cells were arrested in the G1/S transition with Mimosine (T1), released into S phase and harvested every 2 hrs for 8 hrs (T2CT5). (irjs.info)
C) HeLa cells were caught the Rabbit polyclonal to Aquaporin2 G1/S transition with Mimosine (T1), released and harvested every 3 hrs for 6 hrs (T2 and T3). (irjs.info)

Anatomy32

Organisms24

Diseases6

Chemicals and Drugs106

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes61

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science3

Health Care2

Stromal cells5

Vitro12

Proliferation17

Carcinomas6

Inhibition6

Assays6

Endothelial cells6

Epithelial cell3

Malignant cells6

Soft agar2

Follicular3

Apoptosis2

Metastasis2

Mechanisms5

Cultures7

Differentiation8

Specimens3

Basal4

Propagation3

Histological3

Characterization1

Squamous cell carc2

Migration2

Metastatic4

Tumorigenic3

Progression4

Mammalian3

METHODS5

Viability3

Adenocarcinoma2

Results8

Tissue11

Proteins5

Clonal3

Spheroids2

Isolation2

Adhesion3

Pleomorphic adenoma5

Sensitivity1

Adipose cells1

Alpha - smooth mus2

Tissues2

Growth14

Cancer stem2

Gene7

Invasion1

Microenvironment3

Cellular3

Stem4

Vivo4

Lymphoma cells1

Cancerous cells1

Integrity2

Bone1

Analyses2

Fibroblast3

Significantly1

Receptors1

CONCLUSION1

Phosphorylation3

Stimulation1

Lines9

Adenomas1

Establishment1

Functional1

Pathway1

HeLa3