Monolayer cells cultured. Medical search. Frequent questions

It was noted that as opposed to the flattened morphology typically seen in 2D culture, cells grown on the electrospun fibers exhibited a more histotypic rounded 3-dimensional morphology generally observed in vivo. (wikipedia.org)
Not only is the clearing process reversible, making 3D cultures available for further analysis, but the reagent does not alter cell morphology. (corning.com)
The cells with this epitheliumlike morphology expressed marker proteins of taste cells: gustducin and NCAM. (bioone.org)
Adhesion of MSCs to DLT molded in silicone and particularly in collagen initiates polygonal morphology and monolayer formation and enhances not only transcription of CEC typical genes such as ZO-1, Na/K-ATPase, PITX2, and COL-8 but also expression of the corresponding proteins. (lww.com)

VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
Finally, EPB41L4A-AS2 inhibited breast cancer cell proliferation, migration and invasion and induced cell apoptosis in vitro. (cancerindex.org)
Insulin did not alter the inhibitory effect of hydrocortisone on GH3 cell proliferation. (jci.org)
In addition, miR‑205 overexpression reduced and miR‑205 depletion enhanced PC cell proliferation and migration in vitro. (spandidos-publications.com)
Notably, overexpression of RUNX2 partially reversed the inhibitory effect of miR‑205 on PC cell proliferation and migration in vitro. (spandidos-publications.com)
In addition, the results revealed that miR-205-induced downregulation of RUNX2 was associated with the inhibition of PC cell proliferation and migration. (spandidos-publications.com)
We also showed that dose-dependent induction of proliferation was connected with changes in the expression of MKI67, CCND1 and CCNE1 genes in well- and poorly differentiated cancer cells. (spandidos-publications.com)
Our study revealed that Ang II influences EC cells in terms of cancer-related processes, and is responsible for increased proliferation, reduction in apoptosis, increased mobility and modulation of adhesion potential. (spandidos-publications.com)
Therefore, various biologically active peptides such as angiotensin, affecting cell proliferation, have become a new area of study in endometrial cancer research. (spandidos-publications.com)
Recently, a number of studies have shown that Ang II plays an important role in proliferation, invasiveness and migration of tumour cells, alteration of expression of cancer-related genes, as well as in physiological tissue remodelling. (spandidos-publications.com)
Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)
Biomarkers, cell viability and proliferation were assessed. (ecco-ibd.eu)
Low-level laser therapy has been investigated as a possible stimulus for enhancement of proliferation and differentiation of various cell types, but few reports relate undifferentiated mouse pulp cells (OD-21) response to irradiation in in vitro models. (bvsalud.org)
Irradiation time of group I influenced positively the proliferation and viability of OD-21 cells in late cell culture period. (bvsalud.org)
Proliferation-linked shifts in eligibility of T-cell progenitors, population dynamics, DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)

Upon irradiation of ultraviolet (UV) light, OTS molecules were decomposed and became permissive to the adhesion of PC12 cells via adsorption of an extracellular matrix protein, collagen. (elsevierpure.com)
In tendons, gap junctions modulate collagen production, are involved in strain-induced cell death, and are involved in the response to mechanical stimulation. (ox.ac.uk)
Using FRAP, we showed that human tenocytes form functional gap junctions in monolayer and three-dimensional (3-D) collagen I culture. (ox.ac.uk)
TBD cells embedded in collagen gel formed three-dimensional clusters, which had an internal cavity equipped with a tight junction-like structure, a microvilluslike structure, and a laminin-positive layer surrounding the cluster. (bioone.org)
TBD cells formed a monolayer on collagen gel when they were co-cultured with TMD cells. (bioone.org)
TBD, 20A, and TMD cell lines were maintained in a triple cell co-culture, in which TBD cells were pre-seeded as aggregates or in suspension on the collagen gel containing TMD cells, and 20A cells were laid over the TBD cells. (bioone.org)
Master molds with inverted DLT were produced by 2-photon lithography (2-PL). To measure the mechanotransductive potential of DLT, mesenchymal stem cells were cultivated on silicone or collagen imprints with DLT. (lww.com)
Induced EndoMT cells exhibited up-regulation of mesenchymal markers, including collagen type I and α-smooth muscle actin, and a reduction in endothelial cell and junctional proteins, including von Willebrand factor, CD31, occludin, and vascular endothelial-cadherin. (surrey.ac.uk)
Sensitivity of cells exposed to mTHPC-PDT (0-10 µg ml -1 ) was determined in a novel 3-dimensional collagen gel culture system. (open.ac.uk)

Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)

Cultured human gallbladder myofibroblasts (HGBMF) are used as a feeder layer, providing factors (yet uncharacterized) that support the growth and differentiation of the dog PDEC. (pancreapedia.org)
There is a growing body of evidence that multipotent mesenchymal stromal cells' (MSCs') remarkable therapeutic potential is attributed not only to their differentiation and regenerative capacity, but also to the paracrine effect, underlying their immunomodulatory properties. (biomedcentral.com)
Its effect and effectiveness appear to be highly connected with the differentiation status of the cancerous cells, as Ang II appears to play a crucial role in the early and late stages of malignant transformation. (spandidos-publications.com)
Ongoing and future applications include 100-port valves and systems for optimizing the differentiation of induced pluripotent stem cells, a 10,000 channel yeast MicroChemostat, and a MicroDialysis imager, all with direct injection into a mass spectrometer. (selectbiosciences.com)
should not be cultured from sites where they are part of the normal flora because differentiation of pathogens from normal flora may be impossible. (msdmanuals.com)

Electron microscopy showed that the cells in primary culture had a fine structure identical to liver parenchymal cells in vivo, including the observation of desmosomes and bile canaliculi in intercellular space. (nih.gov)
Unlike 2D environments (e.g. a Petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. (wikipedia.org)
Standard 2D, or monolayer, cell cultures are inadequate representations of this environment, which often makes them unreliable predictors of in vivo drug efficacy and toxicity. (wikipedia.org)
These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo , suggesting an autocrine function for IL-8 secreted in the lumen. (biomedcentral.com)
Furthermore, as part of the Reduce, Refine, Replace approach to in vivo research (described here in the Journal of the American Association for Laboratory Animal Science ), 3D cell culture offers a highly valuable in vitro alternative. (corning.com)
2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
We determined the presence of EndoMT in the pulmonary vasculature in vivo and the functional effects on pulmonary artery endothelial cells (PAECs) undergoing EndoMT in vitro. (surrey.ac.uk)
This simple pellet-based 3D culture system allows for cell aggregation and spheroid formation, facilitating cell-cell and cell-extracellular matrix interactions that better mimic the in vivo cellular environment compared with 2D monolayer cultures. (lu.se)

In a recent study potentiality of human CD34+ stem cells explored by generating in vitro agarose gel 3D model to understand the bone ossification process. (wikipedia.org)

TRI Reagent ® performs well with large or small amounts of tissue or cells and works with many samples including human, plant, yeast, bacterial and viral samples. (sigmaaldrich.com)
The whole process of RNA extraction starting with fresh tissue or cells can be completed in less than one hour. (sigmaaldrich.com)
One of the first recorded approaches for their development was at the beginning of the 20th century, with the efforts of Alexis Carrel to develop methods for prolonged in vitro tissue cultures. (wikipedia.org)
This work focused on the importance of the extracellular matrix and the ability of cultures in artificial 3D matrices to produce physiologically relevant multicellular structures, such as acinar structures in healthy and cancerous breast tissue models. (wikipedia.org)
This early use of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types including Human Foreskin Fibroblasts (HFF), transformed Human Carcinoma (HEp-2), and Mink Lung Epithelium (MLE) would adhere to and proliferate upon the fibers. (wikipedia.org)
In living tissue, cells exist in 3D microenvironments with intricate cell-cell and cell-matrix interactions and complex transport dynamics for nutrients and cells. (wikipedia.org)
These matrices help the cells to be able to move within their spheroid similar to the way cells would move in living tissue. (wikipedia.org)
An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
[6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
However, it is not known if CXCR1 is expressed on the apical or basolateral poles in polarized Caco-2 BBE cells or human intestinal tissue or whether the direction of TLR signalling influences the direction of IL-8 secretion. (biomedcentral.com)
Additionally, we determined the localization of the CXCR1 receptor in Caco-2 BBE cells and human intestinal tissue samples and investigated the role of IL-8 autocrine signalling using transcriptomics. (biomedcentral.com)
Rossi Bilodeau suggests using a tissue clearing reagent, such as the Corning® 3D Clear Tissue Clearing Reagent, to replace this approach for 3D cultures. (corning.com)
Tumor tissue from a rare non-infantile 8-year-old female DIG patient was dissociated and digested using collagenase to establish live cultures. (frontiersin.org)
Cells cultured as 3D were embedded as tissue blocks. (frontiersin.org)
Using immunohistochemistry, the 3D cultures were shown to express markers as in the original DIG tumor tissue indicating that the spheroid cultures were able to maintain the heterogeneity found in the original tumor. (frontiersin.org)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)
In vitro tissue engineering of smooth muscle sheets with peristalsis using a murine induced pluripotent stem cell line. (otago.ac.nz)
Tissue from the upper or lower respiratory tract, sputum samples, and samples obtained by nasopharyngeal washing, bronchoalveolar lavage, and biopsy may be submitted for viral culture. (medscape.com)
Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
Bone marrow stromal cells (BMSC) are multipotent stem cells and are considered good candidates for cell restoration in injured brain tissue. (sld.cu)
Using cryopreserved Mouse Intestinal Organoids enables establishment of intestinal organoid cultures without the need to isolate intestinal crypts from primary tissue, eliminating the need for access to fresh mouse tissue and making it easy to standardize experimental starting materials. (stemcell.com)
Viral titers in ferret nasal wash specimens and nasal turbinate tissue correlated positively with peak titer in Calu-3 cells, whereas additional phenotypic and molecular determinants of influenza virus virulence and transmissibility in ferrets varied in their association with in vitro viral titer measurements. (cdc.gov)

Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)

These three-dimensional cultures are usually grown in bioreactors, small capsules in which the cells can grow into spheroids, or 3D cell colonies. (wikipedia.org)
Moreover, cells grown in 3D exhibit different gene expression than those grown in 2D. (wikipedia.org)
Here we investigated IL-8 secretion in response to TLR signalling using intestinal Caco-2 BBE cells grown in the Transwell system. (biomedcentral.com)
Cells were grown in monolayer culture and exposed to exogenously added insulin for up to 8 d. (jci.org)
Rarely, clinically insignificant asymptomatic shedding of the virus may be grown in culture. (medscape.com)

In the interdisciplinary research of biology and aerospace, the 3D printed-scaffolds are also being used for protecting cells from the effect of gravity during the launching. (wikipedia.org)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers significant implications for the study of cartilage biology and the discovery of potential therapeutic interventions for cartilage-related and associated disorders. (lu.se)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
All isolated poha compounds (aerial parts and fruits) were evaluated for their anti-inflammatory activity with lipopolysaccharide (LPS)-activated murine macrophage RAW 264.7 cells, and tumor necrosis factor alpha (TNF-α)-activated nuclear factor-kappa B (NF-κB) with transfected human embryonic kidney cells 293. (nih.gov)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)

We have used patient-derived, typically resistant, glioma stem-like cells (GSCs) in combination with the previously validated preclinical Inovitro™ TTFields system together with a number of therapeutic DDRi. (nature.com)
Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)
Enrichment of cancer stem cells was observed in neurospheres by FACS using CD133 antibody and RT-PCR. (frontiersin.org)
A spontaneous cell line-like cell culture with high percentage of stem cells has been established from a DIG tumor for the first time. (frontiersin.org)
However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. (lww.com)
Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
Repligut human stem-cell-derived monolayer platforms were used to assess GB004 effects under unstimulated or TNFa stimulation conditions. (ecco-ibd.eu)
Bone marrow stromal cells (BMSC) are adult stem cells with characteristics that make them an attractive candidate in cellular therapy for neurological diseases (1). (sld.cu)
Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers. (lu.se)
Stem Cells. (bvsalud.org)
In the Dentistry field, stem cells derived situated in red and near-infrared region of electromagnetic from dental pulp have been isolated and investigated as spectrum. (bvsalud.org)
Immortalized murine pulp cells (OD-21)6 are considered as the major action mechanism of those lasers.11 undifferentiated mesenchymal stem cells derived from fetal Although this mechanism has not been fully clarified, the most molar papillae7. (bvsalud.org)

In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)

We show that certain viral titer measurements following infection of a human bronchial epithelial cell line are positively correlated with viral titers in specimens collected from virus-inoculated ferrets and employ mathematical modeling to identify commonalities between viral infection progression between both models. (cdc.gov)
Fully active virus laboratories that maintain the appropriate cell cultures weekly and annually test 150 stool specimens of any origin for any enteric viruses are deemed to meet this criterion. (who.int)

3D cell cultures can also be performed on microfluidic devices such as the Organoplate® to generate perfusable 3D tissues, and hanging drop devices to generate 3D spheroids. (wikipedia.org)
One critical aspect of optical imaging is the 3D localization of molecular composites in tissues or cells, typically by using specific antibodies with fluorescent reporters. (nature.com)
Organ-on-chips are miniaturized devices that arrange living cells to simulate functional subunits of tissues and organs. (selectbiosciences.com)

this will not only show penetration but also indicate cell viability and spheroid structure. (corning.com)
Barrier integrity was assessed by Transepithelial Endothelial Electric Resistance (TEER), HIF-1a target genes were assessed in cell lysates, and tight junction formation and epithelial monolayer viability were investigated by immunofluorescence staining. (ecco-ibd.eu)
Cell growth and viability were assessed after 7 and 10 days. (bvsalud.org)
001). Cell viability in all the groups was over 80%, except in group IV at day 7. (bvsalud.org)

Transcriptome analyses revealed that Caco-2 BBE cells respond to stimulation with IL-8 supporting the hypothesis that IL-8 induces G protein-coupled receptor signalling. (biomedcentral.com)
Stimulation of polarized Caco-2 BBE cells with Pam2CSK4 and Pam3CSK4, agonists for TLR2/6 and TLR2/1 from the apical or basolateral sides induced secretion of IL-8 predominantly into the apical compartment regardless of the location of the stimulus, although basolateral stimulation induced smaller amounts of IL-8 compared to apical stimulation. (biomedcentral.com)
In the BioMAP human co-culture assay, cells were treated with GB004 one hour prior to stimulation and incubated for 24-168 hours. (ecco-ibd.eu)
GB004 demonstrated activity in 11 of 12 systems in the BioMAP human co-culture assay, which covers a range of disease-relevant immune and non-immune mechanisms under stimulation conditions. (ecco-ibd.eu)

In 1995, an Editorial in Gastroenterology stated: "… because of differences in relative abundance and ease of study, ductal cells have received less scrutiny than acinar cells… One way to overcome the problems inherent with studies of acutely isolated duct cells is to culture them in vitro… However, whereas monolayers of duct cells in culture have been produced, functional studies have not yet been successful" (7). (pancreapedia.org)
Functional assessment of gap junctions in monolayer and three-dimensional cultures of human tendon cells using fluorescence recovery after photobleaching. (ox.ac.uk)
Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
Clinical and cell-based studies as well as investigations on mice have demonstrated pivotal roles for each SRC in numerous physiological and pathophysiological contexts, underscoring their functional pleiotropy. (frontiersin.org)

The aim of the present study was to investigate gap junction-mediated intercellular communication in healthy human tendon-derived cells using fluorescence recovery after photobleaching (FRAP). (ox.ac.uk)
In both monolayer and 3-D cultures, intercellular communication in isolated cells was significantly decreased when compared with cells forming many cell-to-cell contacts. (ox.ac.uk)

Spheroids and organoids are replacing standard cell culture in many traditional cell-based assays. (corning.com)
However, to maximize the cost savings and time efficiency, scientists must be aware of the factors that influence whether or not cell imaging returns high quality data, for both multiwell plate assays and for creating a digital model from z-stack layers. (corning.com)
Biological assays and gene expression analysis were performed on three cell lines: ISH, MFE-296 and MFE-280. (spandidos-publications.com)
Biological assays were performed on well-[Ishikawa (ISH)] moderately (MFE-296) and poorly (MFE-280) differentiated human adenocarcinoma cancer cell lines, representing the G1, G2 and G3 stage of EC. (spandidos-publications.com)
Here we investigated gene expression and secretion of soluble factors elicited by GB004 treatment on immune cells and epithelial cells, human monolayer assays and immune co-culture studies to further explore its protective mechanism. (ecco-ibd.eu)

Adult rat liver parenchymal cells were isolated by collagenase perfusion and cultured as a primary monolayer in HI/WO/BA, a serum free, completely defined, synthetic culture medium. (nih.gov)

The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
3D cell culture, including the increased use of spheroids and organoids, presents unique cell imaging problems, especially when creating a digital model is necessary for experimental analysis. (corning.com)
Rossi Bilodeau notes that when dealing with organoids, there's an additional step to clear the culture matrix they're embedded in. (corning.com)
Cryopreserved Mouse Intestinal Organoids provide a convenient way to establish or standardize intestinal organoid cultures in your laboratory. (stemcell.com)
Each vial contains 200 mouse intestinal organoids derived from the small intestine of C57BL/6 mice that were cultured in IntestiCult™ Organoid Growth Medium (Mouse) and cryopreserved in CryoStor® CS10. (stemcell.com)

Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
Zhang et al ( 9 ) revealed that expression of miR-205 was significantly decreased in radioresistant subpopulations of breast cancer cells and loss of miR-205 expression was associated with poor distant relapse-free survival in patients with breast cancer. (spandidos-publications.com)
By contrast, miR-205 expression has been identified to be significantly increased in several human cancer types, including ovarian cancer, endometrial cancer and laryngeal squamous cell carcinoma, in which it was identified to function as an oncoprotein ( 10 , 11 , 13 ). (spandidos-publications.com)
In the human Repligut intestinal epithelium assay, GB004 significantly reduced cell death and improved barrier integrity in response to pro-inflammatory cytokines. (ecco-ibd.eu)

Since the establishment of the first cell line by Gey et al. (intechopen.com)

Additionally, poha isolates compounds were evaluated for their growth inhibitory effects with U251MG glioblastoma and MDA-MB-231 breast cancer cells that harbor aberrantly-active signal transducer and activation of transcription 3 (STAT3), compared to normal NIH-3T3 mouse fibroblasts. (nih.gov)
Cultured eukaryote cells such as human foreskin fibroblasts or vero cell cultures are used to inoculate the specimen. (medscape.com)

Early studies in the 80's, led by Mina Bissell from the Lawrence Berkeley National Laboratory, highlighted the importance of 3D techniques for creating accurate in vitro culturing models. (wikipedia.org)

Promotes monolayer adherent cell culture. (thomassci.com)

In this chapter, we will a) describe how these cells were originally isolated, b) detail methods for their culture, and c) summarize the published secretory studies that used these cells as a model. (pancreapedia.org)
In general, the platforms can be classified in two types of 3D culturing methods: scaffold techniques and scaffold-free techniques. (wikipedia.org)
Methods: Explants of peritoneum were cultured for 1 h with ME (n = 6). (uthscsa.edu)
Methods: Fluorescence microscopy confirmed that mTHPC was incorporated into all three cell types. (open.ac.uk)
Methods: We developed a streamlined protocol for generating chondrocytes from BMSCs in a 3D culture system using an "in-tube" culture approach. (lu.se)

These studies have led to several findings, for example that certain cytokines are generated by beta cells themselves, sometimes under stress, and also that there are probably key factors that render beta cells susceptible to immune attacks. (selectbiosciences.com)

The compound induced cell cycle arrest at low concentrations and apoptosis at higher concentrations. (nih.gov)
The protective role of GB004 on barrier integrity was further supported by immunostaining experiments demonstrating that GB004 treatment protected epithelial cells from TNFa-induced cell apoptosis, improving both epithelial cell number and tight junction protein ZO-1 staining. (ecco-ibd.eu)

CLSM of sections of the explants demonstrated an intact monolayer of cytokeratin positive cells below the sites of ME implantation. (uthscsa.edu)

Specifically, 4β-hydroxywithanolide E (4HWE) inhibited the growth of colon cancer monolayer and spheroid cultures. (nih.gov)

Approximately 300 spheroids are usually cultured per bioreactor. (wikipedia.org)
3D spheroids have been cultured in a lab setting for up to 302 days while still maintaining healthy, non-cancerous growth. (wikipedia.org)
Hypothesize cell location and thus activity within your spheroids to predict ahead where to focus on for results,' she suggests. (corning.com)

The effects of dexamethasone on multiple metabolic functions of adult rat hepatocytes in monolayer culture were studied. (nih.gov)
Accepted: 24 October 2012 cell yield data, our models require that adult DN1 cells divide multiple times before becoming competent to progress into DN2 stage. (lu.se)

SARS-CoV in 1,048 cases investigated for SARS in the were investigated for SARS coronavirus (SARS-CoV) first 5 weeks after the first-generation diagnostic tests through RNA tests, serologic response, and viral culture. (cdc.gov)

Antigenic relations of HFRS-related viruses: PRNT were performed on Vero E6 cell monolayers with rat antisera and cell culture-adapted viruses. (cdc.gov)

We also found that SRC-3 depletion in cultured HESCs results in a significant attenuation in the induction of a wide-range of established biomarkers of decidualization, despite exposure of these cells to a deciduogenic stimulus and normal progesterone receptor expression. (frontiersin.org)

Viral pneumonia can be diagnosed by isolation and identification of the pathogen through viral culture. (medscape.com)
For HSV isolation, either tube or shell vial culture may be used. (medscape.com)

We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)

6), by treating a normal human gallbladder with 0.5% trypsin / 0.2% EDTA for 1 hour to dissociate myofibroblasts (as well as mucosal epithelial cells). (pancreapedia.org)
The MEM was replaced every 2 days until HGBMF were confluent (usually after 1 week), at which time the cells were detached from the plates with 0.25% trypsin / 0.1% EDTA, plated onto 35 mm six-well plates at a density of 10 5 cells per well, and cultured in MEM. (pancreapedia.org)

Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)

To this end, three cell lines were employed: taste bud-derived cell lines (TBD cell lines), a lingual epithelial cell-derived cell line (20A cell line), and a mesenchymal cell-derived cell line (TMD cell line). (bioone.org)
We compiled data from ferrets inoculated with an extensive panel of over 50 human and zoonotic IAV (inclusive of swine-origin and high- and low-pathogenicity avian influenza viruses associated with human infection) under a consistent protocol, with all viruses concurrently tested in a human bronchial epithelial cell line (Calu-3). (cdc.gov)

Advances in cell culture consumables mean that spheroid and organoid culture is now highly amenable to high content screening . (corning.com)

Albumin synthesis and secretion by the cells was measured by a quantitative electroimmunoassay. (nih.gov)
Pretreatment of cell cultures eliminated the effects of the particulates on interferon synthesis. (cdc.gov)

Basal GH secretion was inhibited by insulin (0.7 nM) after a 48-h lag period by approximately 50% (P less than 0.01, vs. untreated control cells). (jci.org)

Further studies are needed that characterize the mechanisms of ME adhesion to, and migration through, mesothelial cells. (uthscsa.edu)

These tests have sensitivity better than that of cultures. (medscape.com)
Ideally cell line sensitivity should be known for all frozen stocks and evaluated whenever fresh cells are resuscitated or received in the laboratory. (who.int)

Our aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth of these tumors. (frontiersin.org)
The idea that cellular survival and growth could be maintained outside the body was recognized as possible almost hundred years ago when the German zoologist Wilhelm Roux described a successful experiment where he cultured chick neural crest in warm saline for a few days [ 1 ]. (intechopen.com)
Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
We show here that, like SRC-2, SRC-3 is expressed in the epithelial and stromal cellular compartments of the human endometrium during the proliferative and secretory phase of the menstrual cycle as well as in cultured HESCs. (frontiersin.org)
These molecular findings are supported at the cellular level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell when endogenous SRC-3 levels are markedly reduced. (frontiersin.org)

Stock cultures of HGBMF can be maintained in 60 mm culture plates (or T75 flasks). (pancreapedia.org)
Perform cell culture expansion and experience 500cm 2 total growth area within the footprint of a standard flask using Thermo Scientific™ Nunc™ TripleFlask™ Treated Cell Culture Flasks. (thomassci.com)

Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
Intranuclear inclusions often exist in cells infected with DNA viruses. (medscape.com)
Cytoplasmic inclusions usually are present in cells infected with RNA viruses. (medscape.com)

Since that Editorial, we have been able to culture and propagate non-transformed and well-differentiated pancreatic duct epithelial cells (PDEC), obtained from the main pancreatic duct of a dog, and have used them as model for pancreatic ductal secretion. (pancreapedia.org)
There is evidence that 2D or 3D culturing conditions have an impact on the cells' secretome, and here we aimed to compare the secretion of cytokines and growth factors in human MSCs from different sources cultured in 2D and 3D conditions and assess their effect on human macrophages polarization in vitro. (biomedcentral.com)
Intestinal epithelial cells (IECs) play a key role in the inflammatory response to colonizing or invading microorganisms via the secretion of interleukin-8 (IL-8). (biomedcentral.com)
Insulin suppresses growth hormone secretion by rat pituitary cells. (jci.org)
The effects of insulin on basal and hydrocortisone-induced growth hormone (GH) secretion were studied in rat pituitary tumor cells (GH3). (jci.org)
Maximal suppression of basal GH secretion was achieved by 0.7 nM insulin, and these effects were prevented by simultaneous exposure of the cells to guinea pig anti-insulin serum (1:2,000). (jci.org)
The results show that insulin exerts a direct, specific inhibitory effect on basal and hydrocortisone-induced GH secretion by GH3 cells unrelated to glucose utilization by the cells. (jci.org)

Fluorescently labeled tenocytes following photobleaching rapidly reacquired the fluorescent dye from neighboring cells, while HeLa cells, which do not communicate by gap junctions, remained bleached. (ox.ac.uk)
1996. Appearance of micronuclei in generation of hela cells irradiated at low doses. (cdc.gov)

Multipotent mesenchymal stromal cells (MSCs) are an extensively researched yet still understudied tool for treating a wide range of pathological conditions, one particular area of research interest being their immunomodulatory properties. (biomedcentral.com)

Shell vial cultures are normally finalized within 2-3 days. (medscape.com)

Differences in growth properties of endometrial cancer in three dimensional (3D) culture and 2D cell monolayer. (otago.ac.nz)
After Ang II treatment, poorly differentiated endometrial cancer cell line acquired a mesenchymal phenotype, which was characterized by induced expression of EMT-related genes (VIM, CD44, SNAI1, ZEB1 and ZEB2). (spandidos-publications.com)
The aim of the present study was to evaluate the influence of Ang II on human endometrial cancer cells. (spandidos-publications.com)

There have been many solutions attempted that have been successfully implemented in cell cultures but are incompatible in clinical applications due to high levels of cytotoxicity or poor bioavailability. (electropages.com)

MSCs are reported to contribute both to the inflammation initiation and to its resolution via complex cross talk with immune cells, most importantly macrophages [ 1 ]. (biomedcentral.com)
Mechanistically, the importance and meaning of these observations needs to be addressed in a suitable and easily manipulable in vitro system consisting of human islets and immune cells. (selectbiosciences.com)

Background: The effect of photodynamic therapy (PDT) on neural cells is important when tumours are within or adjacent to the nervous system. (open.ac.uk)

These experiences have placed me at an intersection between preclinical laboratory efforts to understand relationships between immune effector cells in the central nervous system and synaptic repair during neuroinflammation, and the many steps necessary to bring small molecule technologies into existence as potential drugs. (rochester.edu)
The criterion standard for HSV laboratory testing is the cell culture. (medscape.com)

A negative HSV cell culture result does not rule out HSV infection, particularly if the specimen is from cerebrospinal fluid (CSF) or nonvesicular lesions. (medscape.com)

Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus. (biomedcentral.com)

Conclusion: Neurons in culture can survive mTHPC-PDT under conditions sufficient to kill tumour cells and other nervous system cells. (open.ac.uk)

The third dimension of cell growth provides more contact space for mechanical inputs and for cell adhesion, which is necessary for integrin ligation, cell contraction and even intracellular signalling. (wikipedia.org)
TripleFlask receives the Nunclon™ Delta treatment, a proprietary cell culture surface treatment that offers maximum adhesion for a broad range of cell types. (thomassci.com)

Anatomy20

Organisms3

Chemicals and Drugs32

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes27

Disciplines and Occupations2

Technology, Industry, Agriculture4

Health Care2

Morphology4

Proliferation15

Collagen9

Adherent culture1

Differentiation5

Vivo8

Generating in vitro1

Tissue32

Differentiate1

Grown5

Biology3

Embryonic3

Stem12

Endothelial cell cultures2

Specimens2

Tissues3

Viability4

Stimulation4

Functional4

Intercellular2

Assays5

Collagenase1

Organoids5

Significantly4

Establishment1

Fibroblasts2

Vitro cultur1

Promotes1

Methods5

Beta-Cells1

Apoptosis2

Explants1

Spheroid cultures1

Spheroids3

Adult2

Diagnostic1

Vero1

Depletion1

Isolation2

Murine1

Trypsin2

Viral culture1

Epithelial cell2

Organoid Culture1

Synthesis2

Approximately1

Migration1

Sensitivity2

Cellular5

Flasks2

Viruses3

Secretion7

HeLa Cells2

Stromal cells1

Vial1

Endometrial3

Successfully1

Immune cells2

Neural1

Laboratory2

Specimen1

Intestinal1

Tumour1

Adhesion2