• Macrophages are directly involved in the fight against invading micro-organisms as well as being antigen-presenting cells which activate lymphocytes. (wikipedia.org)
  • The term chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) has been used to identify patients with a chronically progressive or relapsing symmetric sensorimotor disorder with cytoalbuminologic dissociation and interstitial and perivascular endoneurial infiltration by lymphocytes and macrophages. (medscape.com)
  • The LSM 12.125 antibody reacts with the canine form of the 56 kDa transmembrane glycoprotein, CD4, present on the T-helper/inducer subset of normal canine donor peripheral blood lymphocytes. (bdbiosciences.com)
  • The LSM 8.358 antibody reacts with a CD3-like T-cell receptor-associated cell-surface antigen found on thymocytes and peripheral T lymphocytes. (bdbiosciences.com)
  • Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. (thermofisher.com)
  • Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. (thermofisher.com)
  • Mouse monoclonal to CD57.4AH1 reacts with HNK1 molecule, a 110 kDa carbohydrate antigen associated with myelin-associated glycoprotein. (fabretp.org)
  • Mouse monoclonal to CD31.COB31 monoclonal reacts with human CD31, a 130-140kD glycoprotein, which is also known as platelet endothelial cell adhesion molecule-1 (PECAM-1). (bioinf.org)
  • The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes). (exbio.cz)
  • Conventional monoclonal antibodies(mAbs) raised against CD45 have reactivity against all isoforms, while restricted mAB only reacts to specific isotypes (CD45RA, CD45RB, CD45RO). (biomabinc.com)
  • Mouse monoclonal to CD22.K22 reacts with CD22, a 140 kDa B-cell specific molecule, expressed in the cytoplasm of all B lymphocytes and on the cell surface of only mature B cells. (bioskinrevive.com)
  • Eosinophils and T-lymphocytes in late-phase allergic reactions. (mcgill.ca)
  • Histopathologic analysis revealed an ulcerated lesion with a dense mixed infiltrate of eosinophils, variably sized lymphocytes and epithelioid cells extending into submucosa. (bvsalud.org)
  • The resultant inflammatory mediators that are produced induce migration of lymphocytes, eosinophils, neutrophils, and mast cells to the BMZ. (medscape.com)
  • The Dog T Lymphocyte Cocktail is a three-color reagent cocktail designed to identify dog T lymphocytes by direct immunofluorescence staining with flow cytometric analysis. (bdbiosciences.com)
  • Two color flow cytometric analysis of mononuclear cells stained with mouse monoclonal antibodies specific for the human IGF-I receptor, the human insulin receptor, and monoclonal antibodies directed against specific monocyte and lymphocyte subset cell surface antigens revealed that both IGF-I receptors and insulin receptors were present on nearly all monocytes and B-lymphocytes, but were present on only 2% of T-lymphocytes. (utmb.edu)
  • The Navios tetra system delivers an easy-to-use solution for multi-color flow cytometric analysis of lymphocyte subsets as well as CD4+ and CD8+ T cell subsets ratios. (labcyte.com)
  • A placebo-controlled study of the effect of an Eleutherococcus senticosus extract (Eleukokk) on the immune system was performed with 36 healthy volunteers utilising quantitative multi-parameter flow cytometry with monoclonal antibodies directed against specific surface markers of human lymphocyte subsets. (nih.gov)
  • Monoclonal antibodies directed against tumour necrosis factor-α (anti-TNF) have conventionally been the cornerstone of the treatment of moderate-to-severe inflammatory bowel disease (IBD) [ 1 ]. (biomedcentral.com)
  • CD57 expressed on 7-35% of normal peripheral blood lymphocytes including a subset of naturel killer cells, a subset of CD8+ peripheral blood suppressor / cytotoxic T cells, and on some neural tissues. (fabretp.org)
  • Furthermore, MP increased WBC and HGB counts and reduced the ratio of G0/G1 phase obviously, increased the proportion of S phase and G2/M phase in peripheral blood lymphocytes, increased the counts of CD4+ T cells and the ratio of CD4+/CD8+, and reduced the inhibition rate of splenic lymphocytes. (hindawi.com)
  • The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood. (exbio.cz)
  • Human peripheral blood lymphocytes. (lsbio.com)
  • positive control: peripheral blood lymphocytes (PBL), Jurkat human leukemia T-cell line, HPB human leukemia T-cell line, MOLT-4 human leukemia T-cell line. (exbio.cz)
  • PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone M-A251) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). (biolegend.com)
  • The level of The infants with PEM were enrolled in T-lymphocyte subsets in peripheral blood the study after fulfilling a set of inclusion provide information about the development criteria. (who.int)
  • Gnotobiotic calves were injected intravenously with murine monoclonal antibodies (mAb) directed against the BoCD4, BoCD8 or BoWC1 antigens that define the three major T-lymphocyte subpopulations in cattle. (ox.ac.uk)
  • NK-cells are defined as large granular lymphocytes that do not express T-cell antigen receptors (TCR) or Pan T marker CD3 or surface immunoglobulins (Ig) B cell receptor but that usually express the surface markers CD16 (FcγRIII) and CD56 in humans, and NK1.1/NK1.2 in certain strains of mice. (wikidoc.org)
  • Hünig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G: A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. (exbio.cz)
  • Depletion of either of the other two T-cell subsets that expressed the BoCD8 antigen or the BoWC1 antigen present on the gamma/delta T-cells had no demonstrable effect. (ox.ac.uk)
  • If antibody therapy is continued for the full 5 weeks, T cell tolerance can still be established, suggesting that antibody therapy does not prevent lymphocytes from registering the presence of antigen. (ox.ac.uk)
  • Others components of the infiltrated include lymphocytes, plasma cells, granulocytes and mast cells and occasionally it is misdiagnosed as lymphoma on microscopic examination 4,7,12 . (bvsalud.org)
  • In the present study, the time-related circulatory kinetics of hematological profile and bubaline monocyte subsets have been investigated in vaccinated buffalo calves after challenge infections with BuAHV-1. (bvsalud.org)
  • Whole blood samples were collected at 0, 2, 4, 7, 10, 15, 30, and 63 days post-challenge (PCDs) for the analysis of hematological profiles and the enumeration of monocyte subsets via flow cytometry. (bvsalud.org)
  • In summary, the present study characterized, for the first time, the hematological profile and distribution of blood monocyte subsets in vaccinated and non-vaccinated water buffalo in response to experimental infection with BuAHV-1. (bvsalud.org)
  • Although not experimentally proven, our results support the hypothesis of a linear developmental relationship between monocyte subsets. (bvsalud.org)
  • BMI-CD45Ab13, identifies all three major subsets of leukocyte (Granulocyte, monocyte and lymphocyte) and was selected by Professor Leon Terstappen to participate in CANCER-ID Consortium. (biomabinc.com)
  • Enumeration of CD4+ lymphocytes in HIV-positive participants and age-matched controls was performed on cryopreserved whole blood using the method reported by Fiebig et. (cdc.gov)
  • The AQUIOS CL Flow Cytometer System in combination with AQUIOS Tetra reagents provides a Single Platform Technology (SPT) for lymphocyte subset analysis and enumeration without the need for counting beads. (mybeckman.pl)
  • The CD8α and β chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopuplation of mature T-lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). (bdbiosciences.com)
  • The lymphocytic infiltrate which is sparse and poorly organized is comprised almost entirely of thymus derived lymphocytes (T cells) with a normal proportion of helper/inducer (OKT4+) and suppressor/cytotoxic (OKT8+) cells. (nih.gov)
  • The most salient feature in the verum group was a drastic increase in the absolute number of immunocompetent cells, with an especially pronounced effect on T lymphocytes, predominantly of the helper/inducer type, but also on cytotoxic and natural killer cells. (nih.gov)
  • Natural killer cells (or NK cells ) are a type of cytotoxic lymphocyte that constitute a major component of the Innate immune system . (wikidoc.org)
  • type IV-B results from the direct killing of target cells by cytotoxic T lymphocytes (CTL). (mcgill.ca)
  • Phenotypical analyses with newly developed monoclonal antibodies (mAbs) against porcine NKp46 recently revealed that in blood NKp46 - and NKp46 + cells with NK phenotype exist with comparable cytotoxic properties. (biomedcentral.com)
  • In the clinical management of immune deficiency diseases, accurately counting the absolute cell numbers of leukocyte subsets and measuring the percentage of individual subtypes in blood is critical. (mybeckman.pl)
  • The histochemical demonstration of acid phosphatase (ACP) and adenosine triphosphatase (ATP) has been combined with standard immunofluorescence techniques, using a panel of monoclonal and conventional antibodies, to examine lymphocyte and macrophage subsets and their microanatomical relationships within the subcutaneous rheumatoid nodule (RN). (nih.gov)
  • In contrast, most markers (CD3, CD5, CD13, CD14, CD19, CD45, HLA-DR, and TdT) showed strong correlation between PB and BM samples in AML group.Conclusions:The findings of this study suggests that targeted gating strategy for blast population as well as selection of a suitable panel of monoclonal antibodies may be essential for diagnosis of leukemia resulting in similar immunotyping pattern in PB and BM. (medscimonit.com)
  • These monoclonals were generated by immunizing mice with freshly isolated canine thymocytes. (bdbiosciences.com)
  • CD5, also known as Lyt-1, is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (southernbiotech.com)
  • Functional analyses revealed that splenic NKp46 high NK cells produced much higher levels of Interferon-γ and Tumor Necrosis Factor-α upon stimulation with cytokines or phorbol-12-myristate-13-acetate/Ionomycin compared to the other two subsets. (biomedcentral.com)
  • Therefore porcine splenic NKp46 high NK cells represent a highly activated subset of NK cells and may play a profound role in the immune surveillance of this organ. (biomedcentral.com)
  • With CD45 gating, the relative numbers of beads and lymphocyte subsets are enumerated, and their absolute numbers and percentage values are calculated. (cdc.gov)
  • The lack of B-lymphocytes in the testis is significant, since these are the antibody-producing cells of the immune system. (wikipedia.org)
  • T-lymphocytes (T-cells) are white blood cells which take part in cell-mediated immunity. (wikipedia.org)
  • HIV infection is characterized by a decrease and, eventually, a depletion of CD4+ T-lymphocytes (helper T cells). (cdc.gov)
  • Using immunophenotyping, HIV-positive blood samples and age-matched controls were tested for the proportion of lymphocytes that are T cells, B cells, natural killer (NK) cells, CD4+ T cells (helper T cells), and CD8+ T cells (suppressor/inducer T cells). (cdc.gov)
  • CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. (thermofisher.com)
  • Most of these receptors are not unique to NK cells and can be present in other T cell subsets as well. (wikidoc.org)
  • Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). (thermofisher.com)
  • Western blotting analysis of human CD5 using mouse monoclonal antibodies MEM-32, CRIS1, and L17F12 on laurylmaltoside lysates of Jurkat cells and of K562 cells (negative control) under non-reducing conditions. (exbio.cz)
  • CD25 is also expressed on a subset of non-stimulated CD4 + T cells termed T regulatory cells. (biolegend.com)
  • CD4+ T-cells and other lymphocyte subsets in persons infected with human immunodeficiency virus (HIV). (cdc.gov)
  • Furthermore, cross-linking of NKp46 by NKp46-specific mAbs led to a superior CD107a expression in the NKp46 high NK cells, thus indicating a higher cytolytic capacity of this subset. (biomedcentral.com)
  • In the mouse likewise CD27 and CD11b (Mac-1) are used to dissect NK cells into functionally and developmentally different subsets [ 9 ]. (biomedcentral.com)
  • Despite these hints on important functions of porcine NK cells in vivo, so far no investigations on the existence of functionally differing NK-cell subsets have been reported. (biomedcentral.com)
  • Nevertheless, a recent study from our group with newly developed monoclonal antibodies (mAbs) against the activating receptor NKp46 enabled a more comprehensive insight into the phenotype of porcine NK cells and putative subsets [ 20 ]. (biomedcentral.com)
  • In contrast, NKp46 in the pig was shown to divide porcine CD3 - CD8α + NK cells into NKp46 - and NKp46 + subsets in blood and all organs tested [ 20 ]. (biomedcentral.com)
  • CD3 - CD8α + NKp46 - NK cells show phenotypic and functional properties of NK cells although they produce reduced levels of IFN-γ compared to the NKp46 + subset after in vitro stimulation. (biomedcentral.com)
  • Vedolizumab thereby prevents the interaction of α4β7 integrin with mucosal addressin cell adhesion molecule-1 (MAdCAM-1) on the surface of mucosal endothelial cells, and inhibits the migration of T-lymphocytes into the bowel tissue [ 6 ]. (biomedcentral.com)
  • Because of murine origins of NIH-3T3 cell series Compact disc31-expressing NIH-3T3 cells could possibly be useful as immunogen in creation of diagnostic monoclonal antibodies against individual CD31 without the need for Balaglitazone purification of recombinant protein. (bio-cavagnou.info)
  • Mouse monoclonal anti-human Compact disc31 antibody (Biolegend London UK) was added on cells in last focus of 5 μg/ml. (bio-cavagnou.info)
  • The development of skin sensitization is associated with, and requires, the activation and clonal expansion of allergen responsive T lymphocytes and it is these cells that orchestrate the cutaneous allergic reaction. (cdc.gov)
  • Mantle cell lymphoma (MCL) is a lymphoproliferative disorder derived from a subset of naive pregerminal center cells localized in primary follicles or in the mantle region of secondary follicles. (medscape.com)
  • Our results confirm a potential role of NK cells and the different subsets in the pathogenesis of chronic HCV infection. (who.int)
  • Hepatitis C virus (HCV) infection has impaired cellular immune response and circulating NK cells, NK subsets (in- the ability to establish chronic infection virus persistence [8]. (who.int)
  • Studies sug- terized by flow cytometry using anti between the immune response and the gest that HCV inhibits receptor genes in CD3, CD56 and CD16 monoclonal virus replication rate [5] and play a cru- the activation of NK cells [20], and the antibodies. (who.int)
  • The mechanism was perhaps related to inhibiting lymphocyte apoptosis and promoting the balance of Th1/Th2 cell subsets. (hindawi.com)
  • 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. (exbio.cz)
  • Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (southernbiotech.com)
  • The proliferation and trafficking of T lymphocytes in immune responses are necessary events in determining inflammatory responses. (bioskinrevive.com)
  • This study was designed to investigate the role of the L3T4 + T-lymphocyte subset in disease recurrence in the NOD mouse. (diabetesjournals.org)
  • From these findings we conclude that the disease process in the NOD mouse is L3T4 T-lymphocyte dependent. (diabetesjournals.org)
  • CBA/Ca mice may be made tolerant to minor histoincompatible B10.BR skin grafts by treatment with a short course of non-depleting anti-mouse CD4 and CD8 monoclonal antibodies (mAb), during the transplantation period. (ox.ac.uk)
  • Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD5 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. (exbio.cz)
  • Furthermore transfection of cDNA Balaglitazone coding for a particular proteins in NIH-3T3 cell series continues to be performed for reasons apart from immunization of mice e.g. the signaling functional or potential properties from the molecule.18-21 Here we reported cloning of individual Compact disc31 cDNA and steady expression in NIH-3T3 mouse fibroblast cell line for forthcoming experiments to create monoclonal antibodies against Compact disc31. (bio-cavagnou.info)
  • During the initial phase of the disease, patients demonstrated an increase in the total lymphocyte counts (CD3). (tubitak.gov.tr)
  • The guidelines describe single-platform technology (SPT), a process in which absolute counts of lymphocyte subsets are measured from a single tube by a single instrument. (cdc.gov)
  • T-lymphocyte counts, with special emphasis and stunted children has not changed dra- on the effect of nutritional rehabilitation. (who.int)
  • Immunity to bovine virus diarrhoea virus in calves: the role of different T-cell subpopulations analysed by specific depletion in vivo with monoclonal antibodies. (ox.ac.uk)
  • Defective proximal TCR signaling inhibits CD8 + tumor-infiltrating lymphocyte lytic function. (southernbiotech.com)
  • Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. (biolegend.com)
  • The distribution on lymphocytes for canine is similar to that found for both human and monkey, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers. (bdbiosciences.com)
  • The Cambridge Biotech HIV-1 Western Blot Kit is manufactured by Calypte Corporation from HIV-I propagated in an H9/HTLV-IIIb T-lymphocyte cell line. (cdc.gov)
  • The focus of Dr. Berg's work is on the signaling proteins and pathways regulating T lymphocyte development, differentiation, activation, and migration, with a strong emphasis on T-cell receptor (TCR) signaling. (cuanschutz.edu)
  • All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. (bio-rad-antibodies.com)
  • In the current study it is shown that this NKp46 high phenotype was correlated with an increased expression of CD16 and CD27 compared to the CD8α + NKp46 - and NKp46 + NK-cell subsets in spleen and blood. (biomedcentral.com)
  • A considerable number of phenotypically and functionally different NK-cell subsets have been identified up to date [ 5 ]. (biomedcentral.com)
  • Upcoming potential applications of T cell targeted immunoPET consist of monitoring immune system cell subsets in response to immunotherapy, autoimmunity, and lymphoproliferative disorders, adding general to preclinical immune system cell monitoring. (bioskinrevive.com)
  • Direct cell labeling is suffering from restrictions of radionuclide half-life, probe dilution Kaempferol because of cell department, and potential dangerous effects because of the radiosensitivity of lymphocytes. (bioskinrevive.com)
  • Monoclonal antibodies and T-cell hybridomas : perspectives and technical advances / editors, Günter J. Hämmerling, Ulrich Hämmerling and John F. Kearney. (who.int)
  • A low ratio of CD4+ (helper) any chromosomal or hereditary disorder lymphocytes relative to CD8+ (suppressor) that caused the malnutrition. (who.int)
  • The following product was used in this experiment: CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscience™ from Thermo Fisher Scientific, catalog # 25-0114-82, RRID AB_469590. (thermofisher.com)
  • The following product was used in this experiment: CD279 (PD-1) Monoclonal Antibody (J43), Biotin, eBioscience™ from Thermo Fisher Scientific, catalog # 13-9985-82, RRID AB_467023. (thermofisher.com)
  • All enrolled thymic lymphocytes is widely accepted as infants were breastfed in addition to receiv- an indicator of the depression of thymus- ing some traditional foods, according to dependent immune competence associated their age. (who.int)
  • Thymus, thymic hormones and T lymphocytes / edited by F. Aiuti, H. Wigzell. (who.int)
  • B-lymphocytes take part in the adaptive immune response and produce antibodies. (wikipedia.org)
  • Raman C: CD5, an important regulator of lymphocyte selection and immune tolerance. (exbio.cz)
  • Ocular cicatricial pemphigoid (OCP) is one of the subsets of mucous membrane pemphigoid (MMP), a group of systemic autoimmune diseases characterized by T-lymphocyte dysregulation, the production of circulating autoantibodies directed against a variety of adhesion molecules in the hemidesmosome-epithelial membrane complex, and the production of proinflammatory cytokines and immune system activation markers. (medscape.com)
  • 90% of binding of human lymphocytes to high endothelial venules (HEV) in frozen sections of peripheral, but not mucosal lymphoid tissue. (bdbiosciences.com)
  • Single-platform technology (SPT) is designed to enable de- system and managing the health care of persons infected with terminations of both absolute and percentage lymphocyte sub- human immunodeficiency virus (HIV) ( 1-4 ). (cdc.gov)
  • The molecular definition of these autoantigens facilitates the classification and characterization of subsets of OCP. (medscape.com)
  • Although the mechanism of action of vedolizumab is not fully understood, it is presumed to act primarily by binding to α4β7 integrin, which is predominantly expressed by a subset of gastrointestinal-homing T-lymphocytes. (biomedcentral.com)
  • On the other hand, a general decrease in the proportion of lymphocytes was observed early in the post-infection, both for the VAX-1 and VAX-2 groups, while in the CNT group, the decrease was observed later at +30 and +63 PCDs. (bvsalud.org)
  • A subset of patients with clinical features similar to OCP also has been shown to have autoantibodies against epiligrin, which is identified as laminin 5, a ligand for alpha 6 beta 4 integrin, and autoantibodies to the alpha 6 integrin subunit. (medscape.com)
  • T-lymphocyte subsets were measured 6 months after disease onset by flow cytometry using fluorescein isothiocyanate-labeled specific monoclonal antibodies. (cdc.gov)