Molecule jurkat cells. Medical search. Frequent questions

small-molecule chemical inhibitors of LUBAC, HOIPIN-1 and HOIPIN-8. (moonphase2018.com)
The design and synthesis of such potential inhibitors was guided by SAR studies of peptide based inhibitors such as "Q-VD-O-Ph", as well as small-molecule inhibitors based upon the isatins scaffold. (wright.edu)
Herein, it is shown that by increasing the functionality of such oxindole derived inhibitors from two points of variability (e.g., 1-(2,6-difluorobenzyl)-3-((pyridin-4-yl)methylene)indolin-2-one), to three points of variability by adding an electron-withdrawing group such as a chlorine atom at the C-5 position, the potency of the molecules against apoptosis was approximately increased up to 2-fold. (wright.edu)
Kaiso and prognosis As expected for a transcriptional aspect, the Kaiso Inhibitors,Modulators,Libraries protein is often discovered while in the nucleus of quite a few tumor or non tumor derived mammalian cell lines. (screeninglibraries.com)
Activation of caspases in response to treatment method with Inhibitors,Modulators,Libraries extracts To achieve insights to the potential mechanisms of apoptosis concerned, caspase 3 seven exercise at the same time as pro tein expression of caspase eight and 9 have been measured for that six most potent extracts in HeLa cells just after sixteen h of remedy. (screeninglibraries.com)
Expression of GFP (up to 300-fold over background) and luciferase only occurs in the presence of active NF-κB signaling, enabling screening for genetic and/or small molecule inhibitors and activators of the NF-κB signaling pathway. (systembio.com)

Flow cytometry verified that the apoptotic activity of AMP-WF3 on Jurkat cells was significantly higher than that of HDF and PBMCs. (celljournal.org)
The goals for advances in flow cytometry are clear: measure as many relevant target molecules per cell as quickly as possible. (nature.com)
Fluorescence and isotope tagging are the principal means for measuring antibody binding to cells in flow cytometry. (nature.com)
We used flow cytometry, coimmunoprecipitation, RNA sequencing, mass spectrum, chromatin immunoprecipitation and Gdf15 -/- , OT-I and GFP transgenic mice to demonstrate the effects of GDF15 on Treg cells and related molecular mechanism. (bmj.com)
Moreover, modulation of cytokine production and secretion was examined in the different immune cell types and co-cultured THP-1 and HaCaT keratinocytes by ELISA or flow cytometry. (uni-greifswald.de)

Imidazo[1,2-b]pyrazole-7-carboxamides induce apoptosis in human leukemia cells at nanomolar concentrations. (celljournal.org)
Previously, 3-(2,6-difluorobenzylidene)-5-nitroindolin-2-one has been shown to inhibit apoptosis in human Jurkat T cells at 5 μM activity. (wright.edu)

In the present study, the anticancer activity of pleurocidin-like peptide WF3 isoform X2 (AMP-WF3), from the Poecilia Mexicana fish, against leukemic cell line Jurkat was evaluated, and the cytotoxicity compared with the effects on normal cells, including peripheral blood mononuclear cells (PBMCs) and human dermal fibroblast (HDF) cells. (celljournal.org)
PFT-μ inhibits proliferation of leukemic cell lines and primary blasts Leukemic cell lines and primary cells from AML patients were exposed to different concentrations of PFT-μ (0. (tech-strategy.org)
Hence PFT-μ exerted different impacts in cell apoptosis and cycle in both leukemic cell lineages. (tech-strategy.org)
Thus metabolic Homoharringtonine plasticity in leukemic cell response to environmental factors contributes to chemoresistance and disease recurrence. (bioskinrevive.com)

Jurkat T cells encapsulated with a double layer of PSS-GNR/PAH (PSS-GNR/PAH@Jurkat) showed the highest rate of cell proliferation when compared to 24-h encapsulated cells. (springer.com)
In cell coculture assays, ICOVIR-15K-cBiTE-mediated oncolysis resulted in robust T-cell activation, proliferation, and bystander cell-mediated cytotoxicity. (aacrjournals.org)
Tilapia's T-cells, through dynamic proliferation and apoptosis, have formed a typical primary and memory immune response mechanism, of which the complete activation of the fish T-cells depends on the synergistic effect of the first and second signals, and is co-regulated by Ca2+-NFAT, MAPK/ERK, The NF-kB and mTORC1 pathways. (ecnu.edu.cn)
Next, the selective depletion model suggests that IgM+ B cells can present antigens to T-cells, ensuring the activation and proliferation of T-cells. (ecnu.edu.cn)
Functionally, the fish T-cells feature the cytotoxic function of specifically killing targeted cells, and can assist with the proliferation and antibody secretion of IgM+ B-cells. (ecnu.edu.cn)
whereas the glutamine deprivation or inhibition of the glutaminase GLS1 results in impairment of T-cell proliferation and effector function. (ecnu.edu.cn)
Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
The evaluated functional avidity of these TCRs positively correlated with cell proliferation, cytokine production, and WT1-specific cytotoxicity of the TCR-transduced CD8 + T cells in response to WT1 antigen. (oncotarget.com)

Carcinoembryonic antigen (CEA) inhibits NK killing via interaction with CEA-related cell adhesion molecule 1. (uams.edu)
Adhesion molecule pairs, such as CD2-CD58 and CD11a-CD54, induce a transient adhesion of T cells to APCs, after which the T-cell receptor (TCR) scans the cell surface for antigens presented in the context of human leukocyte antigen (HLA) molecules. (ashpublications.org)

Our results highlight a new role for LFA-1 in the core architecture of the IS that can be exploited to study the spatiotemporal redistribution of surface receptors on SLBs, thereby extending the potential of E6.1 cells and their derivatives for fine-scale imaging studies. (ox.ac.uk)
2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. (systembio.com)
Dietary sugars trigger the production of insulin or insulin-like peptides which are sensed by Insulin receptors at the cell surface and initiate a kinase cascade, leading to the activation of the downstream kinase Akt (PKB), the translocation of GLUT4 glucose transporters to the cell surface, and ultimately glucose uptake. (biomedcentral.com)
Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)

Figure1A) 1 and facilitated BMSC differentiation into osteoblast-like cells (Figure 1B 1 The BMSC-ECM culture model contained key bone marrow components including ECM BMSC osteoblast-like cells and factors released by BMSC and osteoblast-like cells. (bioskinrevive.com)
This chapter aims to explore this theme in more depth, beginning with an outline of the molecular events that dictate the differentiation of T helper (Th) cells at the outset of adaptive immune responses in regional lymph nodes. (biomedcentral.com)
Much of the remainder of the discussion focuses on the different ways in which, in the longer term, the chronic inflammatory process influences maturation, differentiation, and function of effector T cells at sites of inflammation. (biomedcentral.com)
There is now good evidence that there exists a coordinated programme of molecular events initiated at the outset of T-cell differentiation that leads to the generation of CD4 + Th effector cells [ 1 ]. (biomedcentral.com)

Cynaropicrin inhibits the activation of major adhesion molecules CD29 and CD98 involved in inflammation, but not CD43. (wikipedia.org)
Fig. 2: Anti-CD80 TKMG48 enables PD-1 to inhibit T cell activation in the presence of CD80. (nature.com)
Fig. 3: Anti-human CD80 TKMF5 enables human PD-1 to inhibit T cell activation in the presence of human CD80 by dissociating cis duplex of human PD-L1 and CD80. (nature.com)
The enhancement in early signaling E6.1 LFA-1high cells did not affect expression of the early activation marker CD69 but led to an increase in IL-2 expression. (ox.ac.uk)
Hence, it is possible that the nickel-chelating lipids act as ligands to the cell's TCRs, an interaction that needs to be blocked to avoid unwanted cell activation. (ox.ac.uk)
Determination of caspase-3 activation revealed a dose-dependent increase of the cleaved active form of caspase-3 in NALM-6 cells after treatment with 3 4 and 5?μ PFT-μ for 24?h (Physique 2c). (tech-strategy.org)
CD6 attenuates early and late signaling events, setting thresholds for T-cell activation. (ox.ac.uk)
The T lineage glycoprotein CD6 is generally considered to be a costimulator of T-cell activation. (ox.ac.uk)
Calcium responses, and also late indicators of T-cell activation such as IL-2 release, were also diminished in TCR-activated Jurkat cells expressing human CD6, compared with CD6-deficient cells or cells expressing a cytoplasmic deletion mutant of human CD6. (ox.ac.uk)
Thus far, the results indicate that RANKL-mediated EMT in 4T1 and NMuMG cells occurs via activation of the NF-κB p65 subunit. (pdgfreceptor.com)
FADD therefore acts as an adapter molecule, allowing caspase-8 to become recruited to the cytoplasmic region of Fas following receptor activation. (bdbiosciences.com)
Although only two SpeC residues (Tyr(15) and Arg(181)) are critical for activation of virtually all human CD3(+) T cells, a larger cluster of four hot spot residues are required for interaction with Vbeta2.1. (uwo.ca)
In the 2D3, the precise TCR functional avidity of transduced TCRs is easily evaluable by the expression of green fluorescent protein (GFP) reporter gene driven by nuclear factor of activated T cells (NFAT) activation via TCR signaling. (oncotarget.com)
However, it is unknown whether this synapse is fully assembled and leads to T-cell activation by enabling interaction between the T-cell receptor (TCR) and human leukocyte antigen class II (HLA-II). (ashpublications.org)
We established a novel rosetting model by coculturing HLA-II-matched peripheral blood mononuclear cells with HL cell lines and showed IS formation with activation of rosetting T cells. (ashpublications.org)
HLA-II downregulation by class II transactivator knockout did not affect the extent of rosetting, but almost completely abrogated T-cell activation. (ashpublications.org)
Intriguingly, the level of CD58 expression correlated with the extent of rosette formation, and CD58 knockout or CD2 blockade reduced both rosette formation and T-cell activation. (ashpublications.org)
In conclusion, T-cell rosetting in HL is established by formation of the IS, and activation of rosetting T cells critically depends on the interaction of both TCR-HLA-II and CD2-CD58. (ashpublications.org)
In addition, alterations in the protein activation levels were analyzed in plasma-treated Jurkat, THP-1 cells and primary monocytes. (uni-greifswald.de)
CD43, one of the most abundant glycoproteins on the T cell surface, has been implicated in selection and maturation of thymocytes and migration, adhesion, and activation of mature T cells. (mssm.edu)
Overexpression of Cbl in Jurkat cells inhibited the CD43-dependent activation of the mitogen-activated protein kinase (MAPK) pathway and AP-1 transcriptional activity, confirming nevertheless a negative role for Cbl in T cell signaling. (mssm.edu)
However, under normal conditions, PKC activation resulting from CD43 engagement was required to activate the MAPK pathway, suggesting that phosphorylation of Cbl on serine residues by PKC and its association with 14-3-3 molecules may play a role in preventing the Cbl inhibitory effect on the Ras-MAPK pathway. (mssm.edu)
Indeed, there are now compelling data to suggest that chronic cytokine activation may contribute substantially to the phenotype and effector function of synovial T cells. (biomedcentral.com)
I conclude by speculating about how our understanding of T-cell activation in chronic inflammation may influence future therapy, and discuss this in the context of the prevailing view that in a susceptible host, chronic inflammatory disease occurs through a failure of regulatory T cells to downregulate the inflammatory process. (biomedcentral.com)

Our study shows that Jurkat human T cells have a molecule that reacts with both anti-CR2 antibodies and the third component of complement, C3. (nih.gov)
Immunoprecipitation experiments with anti-CR2 antibodies and SDS-PAGE analysis reveal a protein with an apparent molecular mass of 155 kDa which is higher than the one seen in B cells. (nih.gov)
Historically, these cells have proven to be convenient target cells in studies assessing the effector functions mediated by monoclonal antibodies (mAbs). (invivogen.com)
In this study, we tested the hypothesis that tumor-infiltrating T cells could be more effectively activated and redirected by oncolytic adenoviruses that were armed with bispecific T-cell-engager (BiTE) antibodies. (aacrjournals.org)
More recently, these cells have been exploited in imaging studies to identify key players in immunological synapse (IS) assembly in superantigen-specific conjugates and to track the dynamics of signaling molecules on glass surfaces coated with activating anti-CD3 antibodies. (ox.ac.uk)

Degradation of a protein via the ubiquitin pathway proceeds by two discrete and successive steps: (a) covalent attachment of multiple ubiquitin molecules to the protein substrate, and (b) degradation of the targeted protein by the 26S proteasome complex. (justia.com)
The Jurkat E6.1 clone has been extensively used as a powerful tool for the genetic and biochemical dissection of the TCR signaling pathway. (ox.ac.uk)
Use GFP fluorescence for live cell imaging and FACS, or luciferase activity to quantitatively measure activity of the signaling pathway. (systembio.com)
High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
Here we investigated the role of Cbl in the CD43-mediated signaling pathway in human T cells. (mssm.edu)

Target cells in antibody-dependent cellular cytotoxicity (ADCC) or antibody-dependent cellular phagocytosis (ADCP) , either with peripheral blood mononuclear cells, Natural Killer cells, or Jurkat-derived reporter T cells. (invivogen.com)
Immune function abnormalities in peripheral blood mononuclear cell cytokine expression differentiates stages of cutaneous T-cell lymphoma/mycosis fungoides. (uams.edu)
Here, we report that Treg characterization in HIV-infected patients as CD4 + Foxp3 + and CD4 + Foxp3 + CD127 Low/- cells was similar, indicating that both protocols represent a suitable method to determine the frequency of Tregs in peripheral blood mononuclear cells (PBMC) and gut associated lymphoid tissue (GALT). (openvirologyjournal.com)
METHODS: Bleomycin-challenged mice were either treated with AMTC-CM or CM generated from human skin fibroblasts, human peripheral blood mononuclear cells or Jurkat cells, or were left untreated. (unicatt.it)
The hierarchy of T-cell dysfunction from peripheral blood to inflamed joint suggests that these defects are acquired through prolonged exposure to proinflammatory cytokines such as tumour necrosis factor (TNF)-α. (biomedcentral.com)

The process by which a chemical or physical signal is transmitted through a cell as a series of molecular events is called signal transduction. (screeninglibraries.com)
Studies reveal that chronic exposure of T cells to TNF uncouples TCR signal transduction pathways by impairing the assembly and stability of the TCR/CD3 complex at the cell surface. (biomedcentral.com)

Using DNA microarrays and Northern blots to analyze unstimulated cells, we demonstrate that expression of a cluster of genes, including RAG-1 and RAG-2, is repressed by constitutive signals requiring the adapter molecules LAT and SLP-76. (stanfordhealthcare.org)
The adapter molecule Cbl has been shown to be a negative regulator of Ras. (mssm.edu)
Unlike T cell receptor signaling, the interaction of the adapter protein Cbl with Vav and phosphatidylinositol 3-kinase, resulting from CD43-specific signals, is independent of Cbl tyrosine phosphorylation, suggesting an alternative mechanism of interaction. (mssm.edu)

acute myeloid leukemia cell line U937 and acute promyelocytic leukemia cell line NB4 cells were incubated in human BMSC cell line HS-5 derived CM treated with 10 nM of mitoxantrone (Mito) for 6 days and then maintained in drug-free medium Homoharringtonine for 3 months. (bioskinrevive.com)
This dose of drug was wholly lethal to cell in normal medium but a population of leukemia cells incubated in CM survived the treatment and gave rise to multidrug resistant (MR) subpopulation. (bioskinrevive.com)

Some cationic anti-microbial peptides show a wide range of cytotoxic action versus malignant cells, which may lead to developing a novel group of antitumor medications. (celljournal.org)
Despite the existence of a highly immunosuppressive tumor environment, adenovirus-infected cells can nonetheless be efficiently cleared by infiltrating cytotoxic T lymphocytes (CTL) without compromising tumor burden. (aacrjournals.org)
Four different TCRs of HLA-A * 24:02-restricted Wilms' tumor gene 1 (WT1)-specific CD8 + cytotoxic T lymphocytes (CTLs) were transduced into 2D3 cells and the functional avidities of these four TCRs were evaluated. (oncotarget.com)
Adoptive immunotherapy using tumor-associated antigen (TAA)-specific CD8 + cytotoxic T lymphocytes (CTLs) and/or CD4 + helper T (Th) cells can induce the regression of large established tumor in not only mouse models but also cancer patients [ 1 - 3 ]. (oncotarget.com)

This product is a panel of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in a Jurkat T lymphocyte parental cell line background. (sigmaaldrich.com)
Extensive lymphocyte severe infections is a massive loss of lymphocytes, den- apoptosis has also occurred in humans and animals infect- dritic cells, gastrointestial epithelial cells, and other cell ed with several exotic agents, including Bacillus anthracis , types through apoptosis, or programmed cell death. (cdc.gov)
Prevention of lymphocyte apoptosis, same process by which these cell populations are regulat- through either genetic modification of the host or treatment ed during normal health ( 1 , 2 ). (cdc.gov)

Recent findings in our laboratory and those of others, however, have shown that the EBV receptor is also present on T cells. (nih.gov)
2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. (systembio.com)
In addition, more cells signaled on ligand-free SLBs with copper-chelating lipids instead of nickel-chelating lipids and the signaling was found to be predominantly via T-cell receptor (TCR) triggering. (ox.ac.uk)
Measuring calcium mobilization in single cells responding to superantigen, we found that human T cells expressing rat CD6 react significantly less well compared with T cells not expressing the exogenous receptor. (ox.ac.uk)
T cell receptor-independent basal signaling via Erk and Abl kinases suppresses RAG gene expression. (stanfordhealthcare.org)
01:49.1 while the T cell receptor is localized to the center of the contact. (ibiology.org)
02:58.0 So, in terms of signaling mechanisms 03:00.2 or the actual triggering process, 03:02.1 the T cell receptor, 03:06.0 which is, again, at the heart of the immunological synapse, 03:08.1 provides the immunological specificity, 03:10.1 utilizes what's referred to as non-receptor tyrosine kinases, 03:12.0 so it utilizes a couple of. (ibiology.org)
Recombinant HEK293 cell line expressing full length human CD155, also known as poliovirus receptor (PVR) or nectin-like molecule-5 (NECL-5). (tebubio.com)
GDF15 promotes the generation of peripherally derived inducible Treg (iTreg) cells and enhances the suppressive function of natural Treg (nTreg) cells by interacting with a previously unrecognized receptor CD48 on T cells and thus downregulates STUB1, an E3 ligase that mediates forkhead box P3 (FOXP3) protein degradation. (bmj.com)
Adoptive T-cell therapy with T cell receptor (TCR) -engineered T cells is an attractive strategy for cancer treatment and the success in this therapy is dependent on the functional avidity of the transduced TCRs against targeted tumor antigens. (oncotarget.com)
These preclinical and clinical evidences encourage us to develop T-cell adoptive immunotherapy using genetically engineered T cells that are transduced with a T-cell receptor (TCR) gene specific for TAA. (oncotarget.com)

Therefore, cell encapsulation of T lymphocytes might help mitigate this problem and increase the efficiency of immunotherapy in many diseases. (springer.com)
Mouse lungs were analyzed for content of pro-inflammatory and pro-fibrotic molecules, presence of lymphocytes and macrophages and for fibrosis level (through histological semi-quantitative evaluation and quantitative measurement of collagen content). (unicatt.it)
These findings suggest that inter- the apoptotic destruction of lymphocytes and dendritic ventions aimed at reducing the extent of immune cell apop- tosis could improve outcomes for a variety of severe cells could have a particularly adverse effect on disease human infections, including those caused by emerging outcome. (cdc.gov)

Antigen-presenting cells (APCs) in screening bioassays for new molecules, including mAbs, interfering with immune checkpoints . (invivogen.com)
5-7 These molecules are important components of the immunological synapse (IS), which enables antigen-dependent communication between T cells and antigen-presenting cells (APCs). (ashpublications.org)

MISSION ® lentivirus was used to generate the ten (10) monoallelic HLA cell lines, which express individual HLA-A or HLA-B subtypes on the cell surface via a β2M:HLA fusion protein as described in Nature Biotechnology 35, 765-772, (2017) and as demonstrated via FACS analysis. (sigmaaldrich.com)
Recent studies utilizing immunohistochemistry evaluation of ordinary and tumor tissue unveiled that Kaiso protein is predominantly localized inside the cytoplasm in the cell or is totally absent, though. (screeninglibraries.com)
We show here that these lipids by themselves can induce calcium signaling in T cells, also when having protein ligands on the SLB. (ox.ac.uk)
These include members of the Bcl-2 protein family, course, and extent of programmed cell death will aid man- which have both proapoptotic and antiapoptotic activity agement of patients with severe infections. (cdc.gov)

While Jurkat cells showed no plasma-mediated regulation of cytokine expression, THP-1 cells revealed an increased IL-8 secretion after long plasma time duration (360 s). (uni-greifswald.de)

The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 88042803. (sigmaaldrich.com)
The full HLA panel consists of a Beta-2 microglobulin (β2M) knockout cell line and an additional ten (10) monoallelic HLA expression cell lines. (sigmaaldrich.com)
CompoZr ® Zinc Finger Nuclease (ZFN) technology was used to generate the β2M knockout cell line, which does not express endogenous cell surface MHC Class I HLA molecules (HLA-A or HLA-B) as measured by fluorescence-activated cell sorting (FACS). (sigmaaldrich.com)
An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages. (sigmaaldrich.com)
Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification. (sigmaaldrich.com)
To validate our platform, we monitored the rate of response of the T cells to a control set of sgRNAs and compared the performance of the primary T cell screen directly and side-by-side to a Jurkat cell line analysis. (horizondiscovery.com)
RPMI-1640 Medium has been used for maintaining cell line medium and serves as a medium for different cells. (sigmaaldrich.com)
These information are steady with all the results found in the K562 cell line in which expression of the Kaiso is predominantly cytoplasmic. (screeninglibraries.com)
Monitor NF-κB signaling in Jurkat cells Speed your studies with this pre-built 293 TN-based cell line for monitoring NF-κB signaling in real time. (systembio.com)
Using a mutant Jurkat T-cell line, we showed that PLCγ1 associated with LAT when the constitutive association with SLP76 was blocked. (ewha.ac.kr)
Here we describe a basal signal that controls gene expression profiles in the Jurkat T cell line and mouse thymocytes. (stanfordhealthcare.org)
MO3.13, an immortal human-human hybrid cell line that express phenotypic characteristics of primary oligodendrocytes, was created by fusing a 6-thioguanine-resistant mutant of the human rhabdomyosarcoma RD with adult human oligodendrocytes by a lectin-enhanced polyethylene glycol procedure. (tebubio.com)
The Human Cerebral Microvascular Endothelial Cell Line (hCMEC/D3) was prepared from cerebral microvessel endothelial cells (CECs) by transduction with lentiviral vectors carrying the SV40 T antigen and human telomerase reverse transcriptase (hTERT). (tebubio.com)
This cell line shows a spindle-shaped, elongated morphology similar to primary cultures of brain endothelial cells and also exhibits contact inhibition at confluence when cultured on collagen type I or IV. (tebubio.com)
hCMEC/D3 represents a stable, easily grown blood brain barrier (BBB) model cell line. (tebubio.com)
The RARα Reporter (Luc)-HEK293 Cell Line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (NM_000964). (tebubio.com)
Recombinant HEK293 cell line expressing tetracycline-inducible human indoleamine 2,3-dioxygenase (IDO2), Genbank accession number NM_194294. (tebubio.com)
Here, we show a novel platform cell line, named 2D3, which enables the functional avidity of transduced TCRs to be evaluated efficiently and precisely. (oncotarget.com)
These results showed that 2D3 cell line was a novel and stable tool useful for the efficient and precise evaluation of the functional avidity of isolated and transduced TCRs in developing TCR-based immunotherapy. (oncotarget.com)
Here, the CD4+ T helper cell line Jurkat, the monocyte cell line THP-1 as well as the corresponding primary cells were investigated. (uni-greifswald.de)

2000 ). The encapsulation of cells has been developed to avoid immune rejection in cell/tissue transplantation. (springer.com)
NF-κB is a transcription factor that plays a pivotal role in the highly specific pattern of gene expression observed for immune, inflammatory and acute phase response genes, including interleukin 1, interleukin 8, tumor necrosis factor and certain cell adhesion molecules. (justia.com)
By comparison, Jurkat cells have been used only scantily for imaging on supported lipid bilayers (SLBs) incorporating laterally mobile TCR and integrin ligands, which allow to study synaptic rearrangements of surface molecules and the fine architecture of the mature IS, likely due to limitations in the assembly of immune synapses with well-defined architecture. (ox.ac.uk)
Along with life's evolution, the immune system has become increasingly sophisticated compared with 450 million years ago, when fish took the lead in deriving an adaptive immune system based on T- and B-cells. (ecnu.edu.cn)
02:26.1 that these are at least provisionally stable junctions 02:29.0 -- obviously, in the nervous system, 02:30.2 synapses can be stable for years, 02:32.2 in the immune system 02:34.2 the cells are extremely motile in the steady state, 02:36.3 so when they stop for a few hours 02:39.1 that's quite a stable. (ibiology.org)
However, less is known about the plasma-mediated induction of intracellular signaling pathways in human immune cells, which play a leading part in the process of wound recovery and removal of pathogens. (uni-greifswald.de)
Therefore, this thesis examined the cellular effects of a non-thermal atmospheric pressure plasma treatment on human immune cells using the argon plasma jet kinpen 09. (uni-greifswald.de)
Furthermore, this thesis outlined the impact of non-thermal plasma treatment on the gene expression level of immune cells. (uni-greifswald.de)
Differential expression of distinct target genes was further validated by quantitative PCR in the immune cell lines. (uni-greifswald.de)

Riedl S, Leber R, Rinner B, Schaider H, Lohner K, Zweytick D. Human lactoferricin derived di-peptides deploying loop structures induce apoptosis specifically in cancer cells through targeting membranous phosphatidylserine. (celljournal.org)
In this context, in recent years, IREA researchers, in collaboration with the University of San Antonio, Texas, have provided evidence that electromagnetic fields at frequencies used for cell phones are able to induce adaptive response. (cnr.it)
Overexpression of caspase-8 is sufficient to induce apoptosis in certain cell lines (e.g. (bdbiosciences.com)

Here we have explored whether upregulating the low levels of endogenous LFA-1 expression on Jurkat E6.1 cells through transduction with CD11a- and CD18-encoding lentiviruses can improve IS architecture. (ox.ac.uk)
Downregulation of NFAT3 expression in T cells is mediated by lower chromatin accessibility and enhancer activity in its promoter in comparison with aortic smooth muscle cells expressing endogenous NFAT3. (aai.org)

However, the unusual phenotype of synovial T cells, including their profound proliferative hyporesponsiveness to TCR ligation, has challenged the notion that T-cell effector responses are driven by cognate cartilage antigens in inflamed synovial joints. (biomedcentral.com)

The panel can also be used to develop cell-based assays to evaluate candidate cancer immunotherapies and identify tumor-specific neoantigens. (sigmaaldrich.com)
Targeted blockade of the checkpoint molecule programmed cell death 1 (PD-1) can activate tumor-specific T cells to destroy tumors, whereas targeted potentiation of PD-1 is expected to suppress autoreactive T cells and alleviate autoimmune diseases. (nature.com)

Our findings establish a method to potentiate PD-1 function and propose the removal of restraining mechanisms as an efficient strategy to potentiate the function of inhibitory molecules. (nature.com)

Chen, L. & Flies, D. B. Molecular mechanisms of T cell co-stimulation and co-inhibition. (nature.com)
This is important to avoid "false" signaling events in cell studies with SLBs, but also to better understand the molecular mechanisms involved in T-cell signaling. (ox.ac.uk)
Similar MR cells were generated from SupB15 cells treated with doxorubicin Homoharringtonine (SupB15MR-D) (Figure 1E 1 BMSC releases small molecular weight chemoprotective molecules such as fatty acids [11] or cysteine [12]. (bioskinrevive.com)

Around the contrary, in K562 cells, on Kaiso knock down we observed a signifi cant reduce within the Wnt11 expression. (screeninglibraries.com)
The effect of PFT-μ on particular apoptosis was dependant on AnnexinV/7-AAD staining after incubation with different concentrations of KP372-1 IC50 PFT-μ for 48?h in NALM-6 KG-1a TOM-1 End up KP372-1 IC50 being-13 K562 and Jurkat cells. (tech-strategy.org)

Peptide-treated Jurkat cells were associated with increased expression of P21, and P53 genes. (celljournal.org)
cBiTE expression and secretion was detected in supernatants from ICOVIR-15K-cBiTE-infected cells, and the secreted BiTEs bound specifically to both CD3 + and EGFR + cells. (aacrjournals.org)
Promoter methylation regulates SAMHD1 gene expression in human CD4+ T cells. (uams.edu)
Increased expression of CTLA-4 in malignant T-cells from patients with mycosis fungoides -- cutaneous T cell lymphoma. (uams.edu)
We show that, while forced LFA-1 expression did not affect TCR recruitment to the IS, E6.1 LFA-1high cells assembled better structured synapses, with a tighter distribution of signaling-competent TCRs at the center of the IS. (ox.ac.uk)
In human CD4 + T cells, overexpression of NFAT1 and NFAT3 enhanced and suppressed IL-2 expression, respectively. (aai.org)
NFAT3 downregulation in Jurkat cells using RNA interference technology augmented IL-2 expression, whereas a knockdown of NFAT1, NFAT2, and NFAT4 suppressed it. (aai.org)
Introduction of TBX5 into CD4 + T cells enhanced the expression of NFAT3 and suppressed that of IL-2. (aai.org)
Our data suggest that CD6 is a signaling attenuator whose expression alone, i.e. in the absence of ligand engagement, is sufficient to restrain signaling in T cells. (ox.ac.uk)
It became obvious that plasma exposure modulated the expression of several genes in both cell types. (uni-greifswald.de)
Here, elevated gene expression levels of JUN and FOS in Jurkat cells and increased transcription of JUND in THP-1 cells in response to plasma treatment were made visible. (uni-greifswald.de)

PFT-μ decreases intracellular concentrations of AKT and ERK1/2 in NALM-6 cells Following we performed intracellular staining and fluorescence-activated cell sorting analyses of AKT p-AKT ERK1/2 and p-ERK1/2 kinases in NALM-6 cells to judge whether PFT-μ impacts these two main signaling kinases or their phosphorylation position. (tech-strategy.org)

Analysis of Jurkat and Raji mRNA by PCR demonstrated the presence of this novel splice variant in both cell lines. (nih.gov)
Raji lymphoblast-like cells were established from a Burkitt lymphoma patient. (invivogen.com)
Raji-APC cells stably overexpressing an IC molecule (e.g. hPD-L1) are part of InvivoGen's anti-IC assay (Bio-IC™) . (invivogen.com)

Several major species of E2 enzymes have been characterized in mammalian cells, plants, and yeast. (justia.com)
Next to its well-established anti-bacterial properties, plasma can have lethal as well as stimulating effects on mammalian cells. (uni-greifswald.de)

Research shows that it had a pro-apoptotic activity on cancer cell lines U937, Eol-1 and Jurkat T cells. (wikipedia.org)

Our screen was able to identify novel genes that are essential for the survival of primary T cells and genes that modulate the response of the T cells to the small molecule inhibitor treatment. (horizondiscovery.com)
This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)
A unique feature of Hodgkin lymphoma (HL) is the presence of CD4 + T cells that surround, protect, and promote survival of tumor cells. (ashpublications.org)
1,2 Rosetting T cells are extremely important for tumor cell survival. (ashpublications.org)
First, cell survival and apoptosis induction was assessed in response to non-thermal plasma treatment by growth curves and flow cytometric assays. (uni-greifswald.de)
On the basis of these data, it is proposed that T-cell survival and effector responses are driven by antigen-independent, cytokine-dependent mechanisms, and that therapeutic strategies that seek to restore T-cell homeostasis rather than further depress T-cell function should be explored in the future. (biomedcentral.com)

This size is consistent with that of B cell CR2 mRNA. (nih.gov)
Following substantial development, our screening platform uses freshly isolated and stimulated primary T cells which are first transduced with a lentiviral sgRNA library ahead of the introduction of Cas9 mRNA. (horizondiscovery.com)

Interestingly, when encapsulated cells were co-cultured with THP-1 macrophages, co-cultures exhibited TNF-α production enhancement. (springer.com)

Notably, intratumoral injection of this cBiTE-expressing adenovirus increased the persistence and accumulation of tumor-infiltrating T cells in vivo , compared with the parental virus lacking such effects. (aacrjournals.org)

Jurkat T cells transfected with the non-signaling molecule rat CD48 were found to bind to ligand-free SLBs containing ≥2 wt% nickel-chelating lipids upon which calcium signaling was induced. (ox.ac.uk)

To evaluate cytotoxicity of PFT-μ in non-malignant cells we analyzed BMSC samples of four AML patients as well as PB MNC (n=6) and CD34-positive cell samples (n=5) from healthy donors. (tech-strategy.org)

Migration was analyzed by Boyden chamber assays using Falcon cell culture inserts. (pdgfreceptor.com)

With minimal instrumentation (four 96-well plates and a multichannel pipette), cell-specific codes are added to each tagged molecule within cells through sequential rounds of classical split-pool synthesis. (nature.com)
The key steps in the synthesis of such molecules involved electrophilic substitution of the C-5 position of the isatin nucleus (if necessary), N-alkylation, Wolff-Kishner reduction of the C-3 carbonyl group and finally, Knoevenagel condensation. (wright.edu)
Consequently, ROS-modulation has emerged as an anticancer strategy with synthesis of various ROS-inducing or responsive agents that target cancer cells. (degruyter.com)

Single-cell omics provide insight into cellular heterogeneity and function. (nature.com)
Over the years, characterization and functional studies have revealed the complexity of ROS as signaling molecules that regulate various physiological cellular responses or whose levels are altered in various diseases. (degruyter.com)
Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

It inhibits the production of TNF-α from lipopolysaccharide-stimulated murine macrophage RAW264.7 cells. (wikipedia.org)
However, the co-culture of THP-1 macrophage and PSS-GNR/PAH@Jurkat or PSS/PAH@Jurkat did not enhance TNF-α production. (springer.com)

IC50)=50 μM], while normal cells (PBMCs and HDF) were less susceptible. (celljournal.org)
Median IC50 values in PB MNC and CD34-positive cells were 17.6?μ (range 10.4-42.3) and 15.1?μ (range 8.0-20.0) respectively suggesting a higher resistance of normal hematopoietic and stromal cells to PFT-μ as compared with leukemic blasts. (tech-strategy.org)
Cell routine analyses using BrdU/7-AAD staining revealed a lower life expectancy proportion of cells in S stage following 24 KP372-1 IC50 markedly?h incubation with PFT-μ in concentrations of 4 and 5?μ for NALM-6 and 40 and 60?μ for KG-1a (Shape 2a). (tech-strategy.org)

Here, we demonstrate that CD6 significantly reduces early and late T-cell responses upon superantigen stimulation or TCR triggering by Abs. (ox.ac.uk)
The bacterial superantigen streptococcal pyrogenic exotoxin C (SpeC) primarily stimulates human Vbeta2(+) T cells. (uwo.ca)

Therefore, the benzo[d]oxazole derivative can be used as an anticancer supplement agent that shows therapeutic effects in anticancer agent therapy or radiation therapy, and in this regard, the benzo[d]oxazole derivative can overcome tolerance of the cancer cell to anticancer agent therapy or radiation therapy and enhance apoptotic effects on the cancer cell. (justia.com)
In NALM-6 incubation with PFT-μ at 4 5 and 6.5?μ resulted in 34 59 and 80% apoptotic cells above spontaneous apoptosis (11%) respectively. (tech-strategy.org)
In all investigated cell types the pro-proliferative signaling molecules ERK 1/2 and MEK 1/2 as well as the pro-apoptotic signaling proteins p38 MAPK and JNK 1/2 were activated in a plasma treatment time dependent manner. (uni-greifswald.de)
In contrast to Jurkat and primary monocytes, the anti-apoptotic HSP27 was only induced in THP-1 cells in response to plasma exposure. (uni-greifswald.de)

However, when the tilapia is affected by pathogen, the differentially expressed genes of T-cells are mainly concentrated in the metabolic pathways, and large-scale metabolic reorganization occurs. (ecnu.edu.cn)
Further studies have confirmed that the mTORC1 and MAPK/ERK pathways have undertaken TCR signaling, regulated glutamine metabolism through c-Myc, and promoted T-cell immunity in tilapia. (ecnu.edu.cn)

peptide] complex, providing a cognate TCR stimulation on Jurkat-Lucia™ TCR-derived cells. (invivogen.com)
Recent technological advances have accelerated single-cell analyses, but workflows remain expensive and complex. (nature.com)
This technology will enable researchers to greatly accelerate their studies in primary T cells and opens the possibilities for building increasingly complex screening paradigms applicable to human disease. (horizondiscovery.com)

Furthermore, the data indicate that this molecule binds EBV detected by incubation with biotin-conjugated virus and streptavidin phycoerythrin. (nih.gov)
This observation was further strengthened by the actual fact that pre-incubation with skillet caspase inhibitor Z-VAD-FMK (50?μ for 1?h) significantly reduced apoptosis after PFT-μ in NALM-6 whereas KG-1a cells weren't rescued by Z-VAD-FMK (data not shown). (tech-strategy.org)
After incubation for 24 h, the cells invading the lower surface were counted microscopically. (pdgfreceptor.com)

Unlike wild type Jurkat cells, which express multiple HLA subtypes, the Jurkat HLA panel cell lines express individual MHC Class I HLA subtypes. (sigmaaldrich.com)
These monoallelic HLA cell lines can be used to study peptide binding and presentation in the context of individual HLA subtypes. (sigmaaldrich.com)
Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. (sigmaaldrich.com)
PFT-μ induces cell cycle arrest and apoptosis in leukemic cells To further evaluate the impact of PFT-μ on leukemic cells we performed cell cycle and apoptosis analyses with the cell lines NALM-6 and KG-1a. (tech-strategy.org)
On the one hand it could be shown that primary cells are more susceptible to plasma treatment than the respective cell lines. (uni-greifswald.de)
Therefore, DNA microarray analysis was performed with the cell lines Jurkat and THP-1. (uni-greifswald.de)

The characterization of regulatory T cells (Treg) during HIV infection has become of particular interest considering their potential role in the pathogenesis of the acquired immunodeficiency syndrome. (openvirologyjournal.com)
Although several factors, including the stage of infection and the heterogeneity of the HIV populations studied can account for these differences, technical considerations in the phenotypic characterization of these cells cannot be ruled out. (openvirologyjournal.com)

The benzo[d]oxazole derivative, which is a nuclear factor E2-related factor 2 (Nrf2) inhibitor, is capable of inhibiting activity of Nrf2 that induces an antioxidant enzyme to remove reactive oxygen species (ROS) that kills a cancer cell, thereby increasing production of ROS. (justia.com)

We further investigated this hypothesis, the mechanisms involved, the effects on some lung functional parameters and whether AMTC-secreted effector(s) are specific to these cells and not produced by other cell types, extending the time of analysis up to 28 days after treatment. (unicatt.it)
Precisely how effector T cells initiate and promote the inflammatory process in RA, however, remains far from clear. (biomedcentral.com)

In our validation screen, we performed a drug-gene interaction analysis by exposing an edited pool of primary T cells to prolonged treatment with a sub-lethal dose of a specific small molecule inhibitor. (horizondiscovery.com)
To determine the significance of the interaction between SLP76 and PLCγ1 in resting T cells, we examined molecules associated with PLCγ1 in the absence of both SLP76 and, more specifically, the P1 domain of SLP76. (ewha.ac.kr)

Treating cutaneous T-cell lymphoma with highly irregular surfaces with photon irradiation using rice as tissue compensator. (uams.edu)
For example, excessive inhibition by which tissue remodeling takes place during normal of apoptosis is an underlying mechanism of cancer, while growth and development and the physiologic mechanism an inappropriate increase is seen in some neurodegenera- by which labile cell populations such as gastrointestinal tive diseases and other conditions. (cdc.gov)

Similar expanded subpopulations of low ROS expressing and drug resistant cells were Mouse monoclonal to MAPK p44/42 identified in pre-treatment bone marrow samples from ALL patients with slower response to therapy. (bioskinrevive.com)

In contrast, the changes in P21, P53, and BCL-2 genes differed in PBMCs and HDF cells. (celljournal.org)
For cytokine genes, at least three stages are thought to be required for the acquisition of transcriptional competence in T cells: an initiation phase, a commitment phase, and a phase of acute gene transcription (Fig. 1 ). (biomedcentral.com)

Transcription factor 'Signal inducer and activator of transcription 3' (STAT3) is continuously activated in cancer cells. (wikipedia.org)
The binding sites of T-box transcription factor TBX5 and NK-2 transcription factor-related locus 5 Nkx2.5, which were expressed at higher levels in aortic smooth muscle cells than in T cells, were located within the −387 to +97 NFAT3 promoter region, exhibiting the maximum enhancer activity. (aai.org)

EBV infects cells via the CR2 that was previously thought to be expressed only on the surface of B cells and certain epithelial cells. (nih.gov)

Anatomy22

Organisms6

Diseases4

Chemicals and Drugs119

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes43

Information Science3

Inhibitors6

Flow cytometry5

Apoptosis in human2

Leukemic cell4

Proliferation8

Adhesion molecule2

Receptors4

Differentiation4

Activation24

Antibodies5

Pathway5

Peripheral5

Signal transduction2

Adapter3

Leukemia2

Cytotoxic4

Lymphocyte3

Receptor11

Lymphocytes3

Antigen-prese2

Protein4

Cytokine expression1

Line20

Immune9

Induce3

Endogenous2

Antigens1

Tumor-specific2

Inhibitory1

Molecular3

K5622

Expression11

Fluorescence-activated cell1

Raji3

Mammalian cells2

U9371

Survival6

MRNA2

Macrophages1

Parental1

CD481

Cytotoxicity1

Assays1

Synthesis3

Cellular4

Macrophage2

IC503

Superantigen2

Apoptotic4

Pathways2

Complex3

Incubation3

Lines6

Characterization2

Reactive Oxygen1

Effector2

Interaction2

Tissue2

Monoclonal1

Genes2

Transcription2

Epithelial cells1