Epithelial cellsGenesMonolayerKinaseBiologyColonicProliferationColon CancerVitroApoptosisMRNASilico2022GeneMechanismMuscle proteinsTight junctionCellularMembraneTransport proteinsExpressionSecretionTransmembrane2002PeptidesMechanismsSupernatantIsolatesPermeabilityDisruptionInteractionsUptakePathwaysScaffold proteinCalcium ionsColorectal cancer cellsSignaling moleculesStructuralProcessesMammalianTissuesOccludinInhibitMarkerAdenocarcinomaEndothelial cellsNeuronal cell deathBarrierAntiviralIntracellularBindsBiolInvasionJunctionsDifferentially expressedAntibodiesSynthesisExtractsHeat shock pTranscription factorsInfection
Epithelial cells6
- It was first identified in epithelial cells as a 65 kDa integral plasma-membrane protein localized at the tight junctions. (wikipedia.org)
- In CaCo-2 colonic epithelial cells, the expression of this gene increases with cell differentiation. (aacrjournals.org)
- The mucous lining of the LARYNX, consisting of various types of epithelial cells ranging from stratified squamous EPITHELIUM in the upper larynx to ciliated columnar epithelium in the rest of the larynx, mucous GOBLET CELLS, and glands containing both mucous and serous cells. (lookformedical.com)
- Jiang R, Lonnerdal B. Transcriptomic profiling of intestinal epithelial cells in response to human, bovine and commercial bovine lactoferrins. (ucdavis.edu)
- Methods: Primary mouse enteric neurons (EN) were exposed to a conditioned medium from ACE2-expressing Caco-2 colonic epithelial cells infected with SARS-CoV-2 or treated with tunicamycin (ER stress inducer). (emory.edu)
- Tight junctions (TJs) located at the apical lateral region of adjacent intestinal epithelial cells are largely responsible for regulating the intestinal mucosal barrier. (ecco-ibd.eu)
Genes7
- Several genes isolated from resistant enterococci ( agg, gelE, ace, cylLLS, esp, cpd, fsrB ) encode virulence factors such as the production of gelatinase and hemolysin, adherence to caco-2 and hep-2 cells, and capacity for biofilm formation. (medscape.com)
- Real-time reverse transcription PCR showed that the sputum was positive for the upstream regions of the E protein ( upE ) and orf1a genes ( 6 ). (cdc.gov)
- Its anti-inflammatory activity is mediated by the ability to enter inside the nucleus of host cells, thus inhibiting the synthesis of proinflammatory cytokine genes. (frontiersin.org)
- Then, a protein-protein interaction (PPI) network of these DEGs was established and ten hub genes using a high degree of connectivity have been screen out. (trpv1inhibitor.com)
- Furthermore, Gene Ontology (GO) involving the mGluR1 web biological processes (BPs), molecular functions (MFs), and cellular components (CCs) of these DEGs and their Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways had been also analyzed. (trpv1inhibitor.com)
- Cadherin genes encode a superfamily of calcium-dependent adhesion-related type-1 transmembrane proteins. (biomedcentral.com)
- Whole exome sequencing of patients who experienced severe influenza reveals several genes, including scaffold protein AHNAK, with predicted loss-of-function variants that are also identified in our proteomic analyses. (cdc.gov)
Monolayer4
- Nonheme eiron bioavailability was assessed by Caco-2 cell monolayer 59Fe uptake using an extrinsic labeling method. (usda.gov)
- We added throat swab and sputum samples from the patient to a monolayer of Caco-2, Huh 7, and Vero E6 cells, which we incubated at 37°C in 5% CO 2 . (cdc.gov)
- Here, we show that the IL-1β-induced increase in Caco-2 monolayer TJ permeability was mediated by activation of extracellular signal-regulated kinases 1/2 (ERK1/2) signalling pathway and that inhibition of ERK1/2 activity inhibits the IL-1β-induced increase in Caco-2 TJ permeability. (psu.edu)
- The TJ protein occludin and ZO-1 were disrupted in anoxia-injured monolayer. (ecco-ibd.eu)
Kinase3
- These findings provide new molecular basis for further understanding the mechanisms involved in ATP functions, as a signal transducer and activator of MAP kinase cascades, in Caco-2 cells derived from human colon adenocarcinoma. (conicet.gov.ar)
- The activated Elk-1 translocated to the nucleus and binds to the cis-binding motif on myosin light chain kinase (MLCK) promoter region, triggering MLCK gene activation, MLCK mRNA transcription and MLCK protein synthesis and MLCK catalysed opening of the intestinal epithelial TJ barrier. (psu.edu)
- The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. (thermofisher.com)
Biology2
- The focus of my research is the environmental and molecular biology of allergens and the allergic reaction, including Epidemic Thunderstorm Asthma (ETSA) events, and identify novel inhibitors for allergy prevention and treatment. (edu.au)
- Part of Current molecular biology reports , p. 141-147, 2015. (uu.se)
Colonic3
- Accordingly, immunohistochemistry with specific antibodies shows that HMGCS2 is expressed mainly in differentiated cells of human colonic epithelium. (aacrjournals.org)
- PCDH20 mRNA and protein expression is significantly downregulated in the colonic epithelium of Crohn's disease patients and mice with induced colitis compared with controls. (biomedcentral.com)
- By administering a selective ATF6 activator, the impairment of intestinal barrier integrity and dysregulation of CHOP/β-catenin/p-p120-catenin pathway was reversed in Pcdh20 -ablated mice with colitis and PCDH20 -deficient colonic cell lines. (biomedcentral.com)
Proliferation7
- Studies involving high levels of expression of occludin in cancer cells have shown that occludin mitigates several important cancer proliferation properties. (wikipedia.org)
- 8000 Bahía Blanca, Argentina In the present work, we examined the role of ATP in the activation of the MAP kinases (MAPKs) ERK1/2, JNK1/2 and p38 and their involvement in the modulation of transcription factors and proliferation of human colon cancer Caco-2 cells. (conicet.gov.ar)
- We demonstrate, by confocal microscopy, that stimulation of Caco-2 cells with ATP results in the translocation of active MAPKs to the nucleous where they induce the expression of c-Fos and Jun family proteins, the phosphorylation of ATF-1, ATF-2 and JunD transcription factors and stimulate Caco-2 cell proliferation. (conicet.gov.ar)
- RPS3 knockdown led to a significant decrease in the proliferation, survival, migration and invasion and an increase in the apoptosis of Caco-2 cells. (edu.lb)
- Thus, these cultured cells,referred to as P3E6, reproduced, at least in part, the proliferation and stratification pattern existing in the normal esophagus. (silverchair.com)
- This led to a decrease in the overall number of cells but it did not affect cell proliferation. (silverchair.com)
- Intestinal-specific deletion of Pcdh20 damages epithelial cell proliferation and differentiation and causes morphological changes, including shortened crypts and microvilli. (biomedcentral.com)
Colon Cancer2
- In this study, we aim at determining the expression levels of RPS3 in a colon cancer cell line Caco-2 compared to a normal colon mucosa cell line NCM-460 and study the effects of targeting this protein by siRNA on cellular behavior. (edu.lb)
- These findings might present RPS3 as a selective molecular marker in colon cancer and an attractive target for colon cancer therapy. (edu.lb)
Vitro7
- It is a highly biocompatible hydrogel whose physical properties can potentially be tailored to direct 3D cell growth and differentiation both in vitro and in vivo 10 , 11 , 12 . (nature.com)
- This study aimed to characterize bio-compounds from peanut skin (Arachis hypogaea) and their bioactivity (antioxidant activity, inhibition of lipase, and carbohydrase enzymes) and to evaluate their anti-proliferative properties in colorectal cancer cells (HCT116) upon in vitro digestion. (bvsalud.org)
- Wada Y, Lönnerdal B. Effects of Industrial Heating Processes of Milk-Based Enteral Formulas on Site-Specific Protein Modifications and Their Relationship to in Vitro and in Vivo Protein Digestibility. (ucdavis.edu)
- Suppressing mTOR activity, as well as levels and activity of the downstream translation regulators, eIF4E and eIF4A, delayed breast cancer progression, onset of associated pulmonary metastasis in vivo and breast cancer cell invasion and migration in vitro. (edu.lb)
- This paper confirms that both peptides inhibit in vitro the HMGCoAR functionality and it reports the characterisation of the molecular mechanism through which they modulate the cholesterol metabolism in HepG2 cells. (unimi.it)
- For this purpose, we performed in vitro assays, proving that Lf exerts an antiviral activity against SARS-COV-2 through direct attachment to both SARS-CoV-2 and cell surface components. (frontiersin.org)
- Previous studies have shown that IL-1β causes an increase in intestinal epithelial tight junction (TJ) permeability both in in vivo animal and in vitro cell culture model systems. (psu.edu)
Apoptosis2
- In standard cells, loss or disruption of occludin and other tight junction proteins leads to initiation of apoptosis through extrinsic pathways. (wikipedia.org)
- Knocking down of CEP55 could arrest the cell cycle progression and induce apoptosis in Fn-infected Caco-2 cells. (trpv1inhibitor.com)
MRNA2
- In rats, hepatic ketogenic capacity increases rapidly during postnatal development ( 4 ) or fasting ( 5 ) when the mRNA, protein, and activity of HMGCS2 increase in liver ( 6 - 8 ). (aacrjournals.org)
- Administration of dibutyryl-cyclic AMP to adult rats increases mRNA, protein levels, and enzymatic activity of HMGCS2 in the liver ( 6, 9 ). (aacrjournals.org)
Silico2
- Towards in silico CLIP-seq : predicting protein-RNA interaction via sequence-to-signal learning. (ki.si)
- Interestingly, the in silico results strongly supported the hypothesis of a direct recognition between Lf and the spike S glycoprotein, which can thus hinder viral entry into the cells. (frontiersin.org)
20221
- 2022 Apr 4;2(4):1007-1017. (leibniz-fmp.de)
Gene11
- Recent work, using molecular modeling, supported by biochemical and live-cell experiments in human cells demonstrated that occludin is a NADH oxidase that influences critical aspects of cell metabolism like glucose uptake, ATP production and gene expression. (wikipedia.org)
- Based on these findings, we propose that ketogenesis is an undesirable metabolic characteristic of the proliferating cell, which is down-regulated through c-Myc-mediated repression of the key metabolic gene HMGCS2 . (aacrjournals.org)
- We report the investigation of this case and genetic characterization of the virus on the basis of variation and phylogenetic analyses of the spike (S) gene and the full-length genome from patient specimens and isolates from Caco-2 cells. (cdc.gov)
- Molecular phylogenetic tree and coding region variants for the spike (S) glycoprotein gene of MERS-CoV isolates from South Korea, September 2018, and reference sequences. (cdc.gov)
- Furthermore, external acid environment triggered expression of the GFP reporter gene fused downstream of the cdx 2 intestinal homeogene regulatory sequences in P3E6 transfected cells. (silverchair.com)
- These findings demonstrate that chronic exposure of esophageal keratinocytes to acid pH induces transcription of cdx 2, an intestinal specific homeobox gene known to play a critical role in the differentiation and maintenance of intestinal epithelial functions. (silverchair.com)
- This coenzyme is essential in energy generation and some other processes including formation of disulfide bonds in proteins, regulation of gene expression and detoxification of reactive oxygen species (ROS) (Groneberg et al. (ijbiotech.com)
- This was accompanied by reductions in hepatocyte sirtuin 3 (SIRT3) deacetylase activity, gene expression and protein levels as well as in superoxide dismutase enzyme activity. (emory.edu)
- Not too long ago, the high-throughput gene microarray evaluation of Fn-infected and non-infected Caco-2 cells permits us to discover the global molecular adjustments from transcriptome alterations to somatic mutations, too as epigenetic adjustments (De et al. (trpv1inhibitor.com)
- Gene ontology annotation revealed a number of critical signalling pathways that were differentially expressed in cells treated with LA, including biological processes involved in establishment of cell polarity, molecular functions that regulate junctional structures, and cellular components associated with epithelial repair (cell leading edge, ruffle and apical junctional complex). (ecco-ibd.eu)
- The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
Mechanism4
- Here, we used a chromatin immunoprecipitation assay to study the molecular mechanism responsible for this expression pattern. (aacrjournals.org)
- The mechanism of SARS-CoV-2-induced diarrhea remains unclear. (emory.edu)
- The exact biological mechanism(s) by which the BBB limits drug exposure is not clear, but in addition to epithelial tight junctions, enhanced efflux of chemotherapeutic agents out of the CNS space by endothelial cell membrane transporters in brain blood vessels plays a primary role ( 2 ). (jcancer.org)
- The results of the present study provide novel insights into the molecular mechanism of action of LA on the protection of TJ integrity in anoxic injury, an IBD microenvironment and the potential for a more broad use in important digestive diseases such as IBD. (ecco-ibd.eu)
Muscle proteins2
- The objective of this study was to isolate and characterize beef muscle proteins that enhance nonheme iron availability. (usda.gov)
- Low molecular weight, calcium binding muscle proteins. (lookformedical.com)
Tight junction5
- The nine domains are as follows: N-terminus domain (66 aa) transmembrane domain 1 (23 aa) extracellular loop 1 (46 aa) transmembrane domain 2 (25 aa) intracellular loop (10 aa) transmembrane domain 3 (25 aa) extracellular domain 2 (48 aa) transmembrane domain 4 (22 aa) C-terminus domain (257 aa) The C-terminus domain has been shown experimentally to be required for correct assembly of tight junction barrier function. (wikipedia.org)
- Occludin is an important protein in tight junction function. (wikipedia.org)
- Indeed, MDCK cells lacking occludin and its homolog tricellulin exhibit less complex tight junction strand network and impaired barrier function. (wikipedia.org)
- In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. (thermofisher.com)
- Studies indicate that L-Glutamine supplementation bolsters the expression of tight junction proteins, thus improving gut barrier function and reducing the risk of 'leaky gut' (Li & Neu, 2004). (yourdayly.com)
Cellular4
- The presence of occludin decreased cellular invasiveness and motility, enhanced cellular sensitivity to apoptogenic factors and lowered tumorigenesis and metastasis of the cancer cells. (wikipedia.org)
- The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
- These studies provide novel insight into the cellular and molecular processes that mediate the IL-1β-induced increase in intestinal epithelial TJ permeability. (psu.edu)
- Journal of Cellular and Molecular Medicine , 15 (4), 970-982. (psu.edu)
Membrane7
- Membrane enriched extracts (40 µg lysate) of Caco-2 (Lane 1), Caco-2 treated with IFN Gamma (50 ng/mL for 24hrs) (Lane 2), A-431 (Lane 3), HEL 92.1.7 (Lane 4) and Jurkat (Lane 5). (thermofisher.com)
- ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. (thermofisher.com)
- Modelling the role of membrane mechanics in cell adhesion on titanium oxide nanotubes. (ki.si)
- Disruption of plant plasma membrane by Nep1-like proteins in pathogen/plant interactions. (ki.si)
- The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane. (leibniz-fmp.de)
- Ubiquinone or coenzyme Q (CoQ) is a lipid-soluble molecule of the electron transport chain located in the mitochondrial inner membrane of human cells as well as the cytoplasmic membrane of bacteria. (ijbiotech.com)
- Poor correlation between Caco-2 cells and artificial membrane (PAMPA) assays Note axis scales Balimane et al. (slideshare.net)
Transport proteins1
- They can act as transport proteins, regulator proteins, or activator proteins. (lookformedical.com)
Expression10
- Furthermore, manipulation of occludin content in human cells is capable of influencing the expression of glucose transporters, and the activation of transcription factors like NFkB, and histone deacetylases like sirtuins, which proved capable of diminishing HIV replication rates in infected human macrophages under laboratory conditions. (wikipedia.org)
- Furthermore, studies in which mice were deprived of occludin expression showed morphological stability in several epithelial tissues but also found chronic inflammation and hyperplasia in the gastric epithelium, calcification in the brain, testicular atrophy, loss of cytoplasmic granules in straited duct cells of salivary gland, and thinning of the compact bone. (wikipedia.org)
- HMGCS2 protein expression is down-regulated preferentially in moderately and poorly differentiated carcinomas. (aacrjournals.org)
- RT-PCR studies and PCR product sequencing supported the expression of P2Y2 and P2Y4 receptors in this cell line. (conicet.gov.ar)
- Moreover, ATAD2 silencing suppressed epithelial-mesenchymal transition (EMT), and reduced the expression and enzymatic activity of matrix metalloproteinases (MMPs) in Caco-2 and SW-480 cells. (elis.sk)
- Expression of the endogenous CDX2 protein, detected by western blot and immunocytochemical analysis, correlated with promoter activation. (silverchair.com)
- Vasoactive intestinal peptides (VIP) expression and secretion by EN were assessed by RT-PCR and ELISA, respectively. (emory.edu)
- Results: SARS-CoV-2 infection led to increased expression of BiP/GRP78, a marker and key regulator for ER stress in Caco-2 cells. (emory.edu)
- Our data showed that the expression of centrosomal protein of 55 kDa (CEP55) is substantially greater in Fn-infected Caco-2 cells. (trpv1inhibitor.com)
- The expression of CEP55 was positively correlated using the Fn amount in Fn-infected CRC sufferers, and these individuals with 5-HT Receptor Antagonist Species higher CEP55 expression had an of course poorer differentiation, worse metastasis and decreased cumulative survival price.Identification of DEGsGEO2R was utilized to determine the DEGs amongst Fn-infected and Fn-non-infected Caco-2 samples. (trpv1inhibitor.com)
Secretion2
- The modulation of PCSK9 intracellular processing was also evaluated: only P5 was able to decrease the PCSK9 and HNF1-alpha protein levels leading to a decrease of mature PCSK9 secretion by HepG2 cells. (unimi.it)
- Clearance of small intestinal crypts involves goblet cell mucus secretion by intracellular granule rupture and enterocyte ion transport. (gu.se)
Transmembrane3
- These 5 domains are separated by the 4 transmembrane domains of the protein. (wikipedia.org)
- Some animal proteins, for example, those in the LAT family (TC# 2.A.3.8) including ASUR4 (gbY12716) and SPRM1 (gbL25068) associate with a type 1 transmembrane glycoprotein that is essential for insertion or activity of the permease and forms a disulfide bridge with it. (tcdb.org)
- The structure contains 12 transmembrane helices, with the first 10 consisting of an inverted structural repeat of 5 transmembrane helices like LeuT (TC #2.A.22.4.2). (tcdb.org)
20021
- However, in certain pathological conditions the epithelium undergoes changes that transform cells into different phenotypes ( Tosh and Slack,2002 ). (silverchair.com)
Peptides4
- These factors are short peptides with molecular weights of 1,000 to 7,000 that contain the amino acid histidine. (usda.gov)
- P5 (LILPKHSDAD) and P7 (LTFPGSAED) are two peptides from lupin protein that are absorbed in Caco-2 cells. (unimi.it)
- Experimental Strategies Peptides Synthesis from the control peptide (Desk 1 was completed by solid-phase peptide synthesis utilizing a peptide synthesizer (Tribute Proteins Systems Inc. Tucson AZ) at Louisiana Condition College or university (LSU) peptide synthesis service. (bio-biz-navi.com)
- The purity of the merchandise was examined by HPLC electrospray mass spectrometry (ESI-MS) and high res mass spectrometry (HR-MS). Evaluation from the peptides for purity by HPLC indicated >90% genuine peptides and right molecular ion. (bio-biz-navi.com)
Mechanisms1
- In this study we investigated the mechanisms involved in the induction of oxidative stress and impairment of mitochondrial quality control and mitophagy in hepatocytes by the saturated fatty acid palmitate and Western diet feeding in mice and if their harmful effects could be reversed by the neurotrophic factor glial cell derived neurotrophic factor (GDNF). (emory.edu)
Supernatant3
- Following centrifugation, the digest supernatant was sequentially ultrafiltered using 10K and 1K molecular weight cut-off membranes. (usda.gov)
- We observed a cytopathic effect on the third day after inoculation in Caco-2 cells and confirmed viral replication of MERS-CoV in the supernatant of the infected cells by real-time reverse transcription PCR. (cdc.gov)
- We extracted viral RNA from the specimens and the supernatant of the Caco-2 cells using the Viral RNA Mini Kit (QIAGEN, http://www.qiagen.com ). (cdc.gov)
Isolates2
- We evaluated genetic variation in Middle East respiratory syndrome coronavirus (MERS-CoV) imported to South Korea in 2018 using specimens from a patient and isolates from infected Caco-2 cells. (cdc.gov)
- [ 2 ] According to National Nosocomial Infections Surveillance (NNIS) data from January 2003 through December 2003, more than 28% of enterococcal isolates in ICUs of the more than 300 participating hospitals were vancomycin-resistant. (medscape.com)
Permeability1
- Poor relationship between Caco-2 permeability and log Ko/w r2 = 0.097 THESE THEORIES OF DRUG UPTAKE WERE BIOPHYSICAL, 'LIPID-ONLY' THEORIES Corti et al. (slideshare.net)
Disruption1
- Alternatively, treatment with 10 mM LA prevented disruption of TJ proteins during anoxic injury. (ecco-ibd.eu)
Interactions3
- Oligo-pyrrolinone-pyrrolidines (generic structure 1) have the potential to interfere with protein-protein interactions (PPIs), but to reduce this to practice it is necessary to be able to synthesize these structures with a variety of different side chains corresponding to genetically encoded proteins. (tamu.edu)
- We hypothesized that enterocyte-enteric neuron interactions were important in SARS-CoV-2-induced diarrhea. (emory.edu)
- Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. (cdc.gov)
Uptake3
- Through the inhibition of HMGCoAR activity, P5 and P7 are able to improve the LDLR protein levels leading to a better ability of HepG2 cells to uptake extracellular LDL molecules with a final hypocholesterolaemic effects. (unimi.it)
- Polyphenols and phenolic acids from strawberry and apple may decrease glucose uptake by blocking transport through human intestinal cells, according to a new study. (nutraingredients.com)
- "The results obtained demonstrate that polyphenols, phenolic acids and tannin-rich extracts from strawberry and apple were able to influence glucose uptake into the cells and transport … by inhibiting activities of the glucose transporters," said the authors. (nutraingredients.com)
Pathways2
- In addition, western blot analysis revealed that ATP-induced phosphorylation of MAPKs in Caco-2 cells was dependent on calcium influx and intracellular calcium release, Src-family tyrosine kinases, and partially dependent on the cAMP/PKA and PKC pathways. (conicet.gov.ar)
- However, the signalling pathways and the molecular processes that mediate the IL-1β modulation of intestinal epithelial TJ barrier remain unclear. (psu.edu)
Scaffold protein1
- It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. (thermofisher.com)
Calcium ions2
- Proteins to which calcium ions are bound. (lookformedical.com)
- At higher concentrations, barium ions also displace calcium ions from existing junctions [2]. (blogspot.com)
Colorectal cancer cells2
Signaling molecules1
- The C-terminus also interacts with several cytoplasmic proteins of the junctional plaque and interacts with signaling molecules responsible for cell survival. (wikipedia.org)
Structural2
- One of the main challenges with bioprinting cell laden constructs is the identification of an appropriate bioink, as the material not only needs to have the necessary structural and mechanical properties, but should also protect the cells from damage during printing and ultimately provide them with an appropriate environment to direct or control their phenotype and function. (nature.com)
- Structural basis for the unique molecular properties of broad-range phospholipase C from Listeria monocytogenes. (ki.si)
Processes2
- L-Glutamine is the most abundant amino acid in our bloodstream, powering numerous biological processes such as protein synthesis, kidney acid-base balance, and cell energy (Sido et al. (yourdayly.com)
- The complex processes that direct cell-specific differentiation are not completely understood, but once the differentiation program is activated cells usually maintain their specific phenotype. (silverchair.com)
Mammalian1
- They are members of the rBAT family of mammalian proteins (TC #8.A.9). (tcdb.org)
Tissues4
- Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues. (nature.com)
- A calcium-binding protein that mediates calcium HOMEOSTASIS in KIDNEYS, BRAIN, and other tissues. (lookformedical.com)
- Calcium-binding proteins that are found in DISTAL KIDNEY TUBULES, INTESTINES, BRAIN, and other tissues where they bind, buffer and transport cytoplasmic calcium. (lookformedical.com)
- The research, published in Molecular Nutrition & Food Research , suggests that some polyphenols, phenolic acids and tannins extracted from apples and strawberries may block the transport of glucose across intestinal tissues, potentially blunting post-meal glucose spikes. (nutraingredients.com)
Occludin2
- However, mounting evidence demonstrated that occludin is not only present in epithelial/endothelial cells, but is also expressed in large quantities in cells that do not have tight junctions but have very active metabolism: pericytes, neurons and astrocytes, oligodendrocytes, dendritic cells, monocytes/macrophages lymphocytes, and myocardium. (wikipedia.org)
- The C-terminus of occludin is important in receiving and transmitting cell survival signals. (wikipedia.org)
Inhibit1
- But…narcotics inhibit luciferase, a soluble protein, with the same potency with which they anaesthetise animals, over 5 logs! (slideshare.net)
Marker1
- Hence, ATAD2 could be considered as a novel molecular marker of metastatic colorectal cancer, and it may provide new insights for clinical diagnosis and treatment of colorectal cancer. (elis.sk)
Adenocarcinoma1
- digestive tract adenocarcinoma cell range (Caco-2) as well as the T-leukemia Jurkat cell range had been from the American Type Tradition Collection (Rockville MD USA). (bio-biz-navi.com)
Endothelial cells1
- The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. (thermofisher.com)
Neuronal cell death1
Barrier3
- Serving as a primary energy source for the gut lining cells, L-Glutamine is indispensable for maintaining a healthy gut barrier. (yourdayly.com)
- Destruction of intestinal barrier integrity is one of the first phases of CD pathogenesis [ 2 ], while its repair is the final, yet most important, phase of mucosal healing in CD treatment [ 3 ]. (biomedcentral.com)
- The apical junctional complex comprises tight junctions, adhesion junctions, and desmosomes, which seal adjacent cells, regulate paracellular transportation, and maintain intestinal barrier function, acting as a dynamic mechanical barrier [ 5 ]. (biomedcentral.com)
Antiviral1
- The well-known antiviral activity of Lf has been demonstrated against DNA, RNA, and enveloped and naked viruses and, therefore, Lf could be efficient in counteracting also SARS-CoV-2 infection. (frontiersin.org)
Intracellular1
- Spectrofluorimetric measurements showed that cell stimulation with ATP induced transient elevations in intracellular calcium concentration. (conicet.gov.ar)
Binds2
- Our data demonstrates this peptide binds to Compact disc58-bearing Caco-2 cells aswell as to Compact disc48 (homologous to Compact disc58 in human beings) from rodents. (bio-biz-navi.com)
- This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Biol1
- Nat Cell Biol. (leibniz-fmp.de)
Invasion1
- In this study, we established ATAD2 knockdown in colorectal cancer cell lines by RNA interference and found that silencing of ATAD2 inhibited the migration and invasion ability of Caco-2 and SW-480 cells. (elis.sk)
Junctions2
- ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
- ZO-1 may be involved in signal transduction at cell-cell junctions. (thermofisher.com)
Differentially expressed1
- A calbindin protein that is differentially expressed in distinct populations of NEURONS throughout the vertebrate and invertebrate NERVOUS SYSTEM, and modulates intrinsic neuronal excitability and influences LONG-TERM POTENTIATION. (lookformedical.com)
Antibodies1
- Antibodies to this protein can be produced by patients with myasthenia gravis. (thermofisher.com)
Synthesis1
- therefore, cell-wall synthesis is still possible. (medscape.com)
Extracts2
- Tissue extracts of Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)
- Peanut skin was digested in two sequential phases, and the final content, named phase-1 (P1) and phase-2 (P2) extracts, was evaluated. (bvsalud.org)
Heat shock p1
- Infected cells secreted the DAMP protein, heat shock protein 70 (HSP70), into the culture media, as revealed by proteomic and Western analyses. (emory.edu)
Transcription factors1
- Multiple muscle wasting-related transcription factors are acetylated in dexamethasone-treated muscle cells. (per-olofhasselgren.com)
Infection4
- This activity varied according to concentration (100/500 μg/ml), multiplicity of infection (0.1/0.01), and cell type (Vero E6/Caco-2 cells). (frontiersin.org)
- Understanding host proteins essential for IAV infection can identify targets for alternative host-directed therapies (HDTs). (cdc.gov)
- Of our identified host factors, 54 significantly alter IAV infection upon siRNA knockdown, and two factors, AHNAK and coatomer subunit COPB1, are also essential for productive infection by SARS-CoV-2. (cdc.gov)
- This study provides a comprehensive network model of IAV infection in human cells, identifying functional host targets for pan-viral HDT. (cdc.gov)