Molecular embryonic stem cells. Medical search. Frequent questions

NLRP7 thus appears to function in chromatin reprogramming and DNA methylation in the germline or early embryonic development, functions not previously associated with members of the NLRP family. (nih.gov)
Combinatorial binding in human and mouse embryonic stem cells identifies conserved enhancers active in early embryonic development. (mpg.de)
Zaret's lab previously discovered pioneer factors in 2002 and has been working to better understand their function and role in early embryonic development. (newswise.com)
Human embryonic stem cells (hESc) can also be used to analyze the in vitro development from undifferentiated pluripotent cells leading to terminally differentiated cell types, recapitulating the process of early embryonic development [5] . (plos.org)
The findings reveal the widespread impact of both nicotine and non-nicotine components of tobacco smoke on early embryonic development, according to Bernstein. (ucsf.edu)

1 Interdepartmental Program in Translational Biology and Molecular Medicine. (nih.gov)
I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
I cover mainly cell biology and physiology. (nottingham.ac.uk)
We've entered what can be considered the third phase of the human genome project," said Job Dekker, PhD, Howard Hughes Medical Institute Investigator , professor of biochemistry & molecular pharmacology and co-director of the Program in Systems Biology. (umassmed.edu)
Nature Structural & Molecular Biology 23, 116-124 (2016). (uni-muenchen.de)
We now understand that this pioneer factor, FoxA2, grabs the chromosomal proteins, known as histones, and exposes the DNA region," said the study's corresponding author Kenneth S. Zaret, PhD, the Joseph Leidy Professor in the Department of Cell and Developmental Biology and Director of Penn's Institute for Regenerative Medicine (IRM). (newswise.com)
Max Planck Institute of Molecular Physiology, Department of Systemic Cell Biology, 44227 Dortmund, Germany. (bvsalud.org)
Histochemistry and Cell Biology. (lu.se)
Combining cell biology and electrophysiology, his work has the potential to create personalized disease models for future research. (lu.se)
On completion of the course, the students shall be able to · reflect on societal opportunities and consequences arising from developmental biology research and knowledge, and discuss these with individuals in the same education cycle · reflect on current scientific challenges and ethical issues in developmental and stem cell biology research and how this influences society in general. (lu.se)
The course covers basic principles in developmental biology and molecular genetics with a special emphasis on developmental biology model systems in vertebrates and invertebrates. (lu.se)
The whole course is based on five week-long modules on selected subjects related to issues concerning developmental biology and stem cells. (lu.se)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

This has catalyzed research in both early embryogenesis as a model system for mammalian development as well as regenerative medicine as a renewable source of unspecialized cells which can be converted into nearly any cell type required by a patient. (utexas.edu)
Due to their plasticity and potentially unlimited capacity for self-renewal, embryonic stem cell therapies have been proposed for regenerative medicine and tissue replacement after injury or disease. (wikipedia.org)
In addition to their potential in regenerative medicine, embryonic stem cells provide a possible alternative source of tissue/organs which serves as a possible solution to the donor shortage dilemma. (wikipedia.org)
In addition to retinal cells, other stem-cell derivatives are likely to play an important role in the future of regenerative medicine. (news-medical.net)
The researchers of the paper from Advanced Cell Technology, collaborated with scientists from the Institute of Regenerative Medicine at Wake Forest University School of Medicine, Winston-Salem, North Carolina, and the University of Chicago, Chicago, Illinios. (news-medical.net)
Advanced Cell Technology is a leading biotechnology company in the emerging field of regenerative medicine. (news-medical.net)
We evaluated nine possible scenarios of how embryonic stem cells could be set up to interact with collagen scaffolds when used in tissue engineering or regenerative medicine applications. (rsc.org)
Oct. 10, 2023 Generating specific cell lineages from induced pluripotent stem cells and embryonic stem cells is the holy grail of regenerative medicine. (sciencedaily.com)
The course aims to prepare students for work in a field that includes regenerative medicine and stem cells. (lu.se)

This investigation aimed to characterize the biophysical and molecular properties of exosomes harvested from human embryonic stem cells (hESCs) following the guidelines of the International Society for Extracellular Vesicles. (ucalgary.ca)

Computational analysis of genome-wide DNA methylation during the differentiation of human embryonic stem cells along the endodermal lineage. (mpg.de)
FGF inhibition directs BMP4-mediated differentiation of human embryonic stem cells to syncytiotrophoblast. (mpg.de)

However, the possible differences in proliferation and differentiation capabilities among independently-derived human embryonic stem cells (hESCs) are not well known because of insufficient characterization. (biomedcentral.com)
Human embryonic stem cells (hESCs) possess the ability to self-renew in an undifferentiated state in culture while retaining the ability to differentiate into all of the cell types in the human body. (biomedcentral.com)
These unique capabilities make hESCs a renewable source of a wide range of cell types for potential use in research and cell-based drug screening and therapies for many diseases. (biomedcentral.com)
Here we combine chromatin immunoprecipitation with a massively parallel reporter assay to identify functional enhancers in human embryonic stem cells (hESCs) genome-wide in a quantitative unbiased manner. (biorxiv.org)
Toward this goal, we propose to compare the behavior of nerve cell differentiation in multiple lines of hESCs in one laboratory setting. (ca.gov)
We generated GABAergic interneuron precursors from human embryonic stem cells (hESCs) and grafted them in the hippocampi of rats developing chronic SRSs after kainic acid‐induced status epilepticus. (lu.se)
Through the use of human embryonic stem cells (hESCs), we can mimic brain development towards different regions of the human brain, and thereby investigate the effect of novel genes on neural differentiation. (lu.se)

Feb. 21, 2022 Embryonic stem cells and other pluripotent cells divide rapidly and have the capacity to become nearly any cell type in the body. (sciencedaily.com)
International journal of molecular sciences 2022 0 0. (cdc.gov)

The discovery, published in Nature Genetics , helps untangle mysteries of how embryonic stem cells develop into organs. (newswise.com)
Up to July 2004 he had spent almost 20 years as Professor of Life Sciences, Indiana State University, and Adjunct Professor of Medical and Molecular Genetics, Indiana University School of Medicine. (lifenews.com)

This screen is capable of analyzing the expression of 48 genes simultaneously across dozens of different samples, and our gene list covers all three germ layers that arise during normal embryonic development, the trophectoderm, and epigenetic regulators of chromatin status. (utexas.edu)
We are intrigued by how we can utilize the setting of normal embryonic development to understand tumor initiation. (lu.se)

Removing those key amino acid signatures significantly impaired embryonic development, caused deformities in organs-including the brain and the heart-and resulted in death in the mice. (newswise.com)

Pluripotency factors Oct4 and Nanog play a role in transcriptionally regulating the embryonic stem cell cycle. (wikipedia.org)
A data integration approach to mapping OCT4 gene regulatory networks operative in embryonic stem cells and embryonal carcinoma cells. (mpg.de)
In silico identification of a core regulatory network of OCT4 in human embryonic stem cells using an integrated approach. (mpg.de)
Induces upregulation of the pluripotency network genes (Klf4, Klf2, Nanog, Oct4, Myc, Sox2) and epigenetic-associated proteins (Dnmt3a, Mbd3) in mouse ES cells, and inhibits retinoic acid-induced differentiation (Adamo et al. (stemcell.com)
Whilst LiCl exposure did not significantly alter the proportion of cells expressing markers for stem cells/undifferentiated cells (Oct4, SSEA4), neurons (Neurofilament M), astrocytes (GFAP) or cell cycle phase, the drug caused a 1.4-fold increase in total cell number. (plos.org)
In contrast, exposure to VPA resulted in significant upregulation of Oct4, SSEA, Neurofilament M and GFAP with significant decreases in both G2/M phase cells and cell number. (plos.org)
The key players in mouse embryonic stem cells governing pluripotency versus differentiation are Oct4, Sox2 and Nanog. (lu.se)

Embryonic stem cells of the inner cell mass are pluripotent, meaning they are able to differentiate to generate primitive ectoderm, which ultimately differentiates during gastrulation into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm. (wikipedia.org)
These germ layers generate each of the more than 220 cell types in the adult human body. (wikipedia.org)
In the present study, a side-by-side comparison of the ability to maintain an undifferentiated state and to self-renew under standard conditions, the ability to spontaneously differentiate into cell types of three germ layers in embryonic bodies, and directed differentiation under neural differentiation-promoting conditions was made between three NIH registered hESC lines I3, I6 and BG01V. (biomedcentral.com)

Isolating the inner cell mass (embryoblast) using immunosurgery results in destruction of the blastocyst, a process which raises ethical issues, including whether or not embryos at the pre-implantation stage have the same moral considerations as embryos in the post-implantation stage of development. (wikipedia.org)
Rapid cell division allows the cells to quickly grow in number, but not size, which is important for early embryo development. (wikipedia.org)
Here, we model early human neural tube development using human embryonic stem cells cultured in a microfluidic device. (nature.com)
Since that time, these cells have given us a wealth of information of how mammalian embryos, including human babies, develop in the womb and how development continues following birth. (scitizen.com)
Just as importantly, research on these cells has been providing us with what happens when foetal development goes wrong, either because of a genetic defect or one that is imposed, for example, by a chemical or drug. (scitizen.com)
However, purification of DE in mammals has not been achieved, limiting the molecular "definition" of endoderm, and hindering our understanding of DE development and attempts to produce endoderm from sources such as embryonic stem (ES) cells. (uthscsa.edu)
This work should accelerate our understanding of mechanisms regulating DE development in mice and humans, and guide further use of ES cells for tissue replacement. (uthscsa.edu)
Stem Cells and Development , 21 (12), 2273-2287. (uthscsa.edu)
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. (thermofisher.com)
The NIH Molecular Libraries Roadmap Initiative wishes to solicit HTS assay applications from the scientific community from investigators who have the interest and capability to work with the Molecular Libraries Probe Production Centers Network (MLPCN) in support of chemical probe development. (nih.gov)
Notch receptors and Notch ligands were detected in virtually all cells throughout EB development. (karger.com)
Our results indicate that this transactivator is toxic to ES cells and raise the need for the development of other strategies for stable and inducible expression of genes in ES cells. (muni.cz)
The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. (mdpi.com)
In 2011, molecular biologists led by Mitinori Saitou at Kyoto University reported that they had managed to recreate the first stages of sperm development in a dish. (scientificamerican.com)
They coaxed mouse embryonic stem cells to become cells that resembled primordial germ cells (PGCs)-an important stage in the development of both eggs and sperm. (scientificamerican.com)
Now, Xiao-Yang Zhao, a development biologist at the Southern Medical University in Guangzhou, and Qi Zhou, a cloning specialist and stem-cell biologist at the Institute of Zoology in Beijing, along with colleagues from Nanjing Medical University, say that they have trumped Saitou's work by carrying out more of the process in a dish. (scientificamerican.com)
For example, Zhou and Zhao report that, on the basis of a genetic analysis, their artificial PGCs were similar to mouse cells at 12.5 days of development. (scientificamerican.com)
And other stages of germ-cell development occurred unexpectedly quickly: in a real mouse, it takes more than 4 weeks for the PGC to become a spermatid, for example, but the Chinese team reports a 14-day interval between artificial PGC and spermatid. (scientificamerican.com)
Molecular signals begin dictating what organs an embryo's stem cells will give rise to in the body-such as the liver or pancreas-within the first two weeks of development. (newswise.com)
Knowing how regulatory gene proteins work during this early stage can help the field better understand how to control the process of cell development for both clinical research and therapeutic purposes. (newswise.com)
Next, the teams translated those findings into a mouse model, deleting the same sequences in mice to see how those changes would affect embryonic development. (newswise.com)
Scientists have gained insight into how second-hand tobacco smoke damages the earliest stages of human embryonic development. (ucsf.edu)
They also established that at least some of the impact was mediated through several molecular pathways known to play a role in differentiation.In one of the pathways, the toxins dramatically increased the activity of a key gene that keeps embryonic stem cells in an undifferentiated state, suggesting that its disruption might be responsible for much of the delay seen in embryonic development. (ucsf.edu)
First, they extended the analysis of gene activity in umbilical cord stem cells previously examined by their University of Connecticut co-authors, determining that tobacco smoke increased the activity of genes that delay the development of mesoderm - the layer of tissue that gives rise to blood, musculoskeletal and cardiovascular systems - as well endoderm and ectoderm, the two other layers of embryonic cells that give rise to the tissues of the body. (ucsf.edu)
They also highlight the power of human embryonic stem cells as a model of human development. (ucsf.edu)
Transcription factors (nurr1, pitx3 and lmx1a) that promote embryonic mouse or chicken dopaminergic development were employed to determine if they would modulate lineage restriction of these adult human progenitors. (scirp.org)
Molecular Therapy - Methods and Clinical Development. (lu.se)
The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)
In particular, it plays an important role in development of hematopoietic stem cells, early blood cells that have the potential to develop into all types of mature blood cells such as white blood cells, red blood cells, and platelets. (medlineplus.gov)
While t(8;21) is important for leukemia development, a mutation in one or more additional genes is typically needed for the myeloid blasts to develop into cancerous leukemia cells. (medlineplus.gov)
Furthermore, the course covers the most important processes in early embryo development, such as fertilisation, cell division, establishment of position information, polarity and asymmetries, and formation of body axes and gastrulation as preconditions for extremity development, regeneration and formation of the body's most important organs. (lu.se)
Our data shows that the STEM-PD product is safe and highly efficacious in reverting motor deficits in preclinical models of Parkinson's disease" says Agnete Kirkeby, who has led the preclinical development of the product. (lu.se)

Mouse embryonic stem (ES) cells are pluripotent cells, meaning that they can give rise to all tissues in the body. (utexas.edu)
Fig. 3: Single-cell transcriptomics of dorsal and ventral MiSTR tissues. (nature.com)
Cells Tissues Organs (2011) 193 (4): 239-252. (karger.com)
Therapeutic potentials of mesenchymal stem cells (MSCs) depend largely on their ability to secrete cytokines or factors that modulate immune response, enhance cell survival, and induce neovascularization in the target tissues. (hindawi.com)
And because of the significant possibility that ESC-derived cells can run amuck, growing out of control or forming improper tissues throughout the body, Geron has already said they will have to monitor the patients for 15 years to assess the danger. (lifenews.com)
The use of olfactory progenitors as a cell-based therapy for Parkinson's disease (PD) would allow harvest without invasive surgery, provide an autologous cell population, eliminate need for immunosuppression and avoid the ethical concerns associated with embryonic tissues. (scirp.org)
Finally, there is a discussion about the mechanisms behind the self-renewal and differentiation of stem cells and the role of stem cells in renewal of the body's tissues. (lu.se)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)

Pluripotency distinguishes embryonic stem cells from adult stem cells, which are multipotent and can only produce a limited number of cell types. (wikipedia.org)
This pluripotency depends on a molecule -- E-cadherin -- hitherto primarily known for its role in mediating cell-cell adhesion. (sciencedaily.com)
If E-cadherin is absent, the stem cells lose their pluripotency. (sciencedaily.com)
In a first step they showed that the pluripotency of these stem cells is directly associated with the cell-adhesion molecule E-cadherin. (sciencedaily.com)
Thus, we have double proof that E-cadherin is directly associated with stem-cell pluripotency. (sciencedaily.com)
This process accelerates upon acquisition of primed pluripotency and returns to low levels in lineage committed cells. (uni-muenchen.de)

Embryonic stem cells (ESCs), derived from the blastocyst stage of early mammalian embryos, are distinguished by their ability to differentiate into any embryonic cell type and by their ability to self-renew. (wikipedia.org)
When provided with the appropriate signals, ESCs initially form precursor cells that in subsequently differentiate into the desired cell types. (wikipedia.org)
According to a 2002 article in PNAS, "Human embryonic stem cells have the potential to differentiate into various cell types, and, thus, may be useful as a source of cells for transplantation or tissue engineering. (wikipedia.org)
A unique and essential property of embryonic stem cells is the ability to self-renew and differentiate into multiple cell lineages. (biomedcentral.com)
Under the same neural differentiation-promoting conditions, the ability of each cell line to differentiate into neural progenitors varied. (biomedcentral.com)
It is also essential to understand how the inherited variation in the sex, stage, quality and genetic background of embryos, as well as environmental influences such as derivation methods and passage procedures can affect the ability of hES cell lines to self-renew and differentiate. (biomedcentral.com)
Researchers have discovered what enables embryonic stem cells to differentiate into diverse cell types and thus to be pluripotent. (sciencedaily.com)
Researchers of the Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch have discovered what enables embryonic stem cells to differentiate into diverse cell types and thus to be pluripotent. (sciencedaily.com)
Mesenchymal stem cells (MSCs) are multipotent stem/progenitor cells which can differentiate to several mesodermal derivatives and possess an ability to secrete factors involved in neovascularization and immunomodulation [ 1 , 2 ]. (hindawi.com)
GATA-3-deficient embryonic stem cells exhibit an enhanced capacity to differentiate into adipocytes, and defective GATA-2 and GATA-3 expression is associated with obesity. (nih.gov)
Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)

abstract = "Embryonic definitive endoderm (DE) generates the epithelial compartment of vital organs such as liver, pancreas, and intestine. (uthscsa.edu)

We will further characterize molecular changes during directed cell differentiation and identify the cells that exhibit a pattern of DNA modification, namely DNA methylation, similar to primary neural cells in human brain. (ca.gov)
We also test the pattern of DNA methylation in different lines of human stem cells. (ca.gov)
By engineering stem cells carrying different levels of methylation, we aim to find the optimal levels of DNA methylation for efficient nerve cell differentiation. (ca.gov)
Epigenetic plasticity underpins cell potency, but the extent to which active turnover of DNA methylation contributes to such plasticity is not known and the underlying pathways are poorly understood. (uni-muenchen.de)
We aimed to investigate whether the molecular subtypes also display distinct methylation profiles. (lu.se)
Conclusions: We have found that breast cancers of the basal-like, luminal A and luminal B molecular subtypes harbour specific methylation profiles. (lu.se)

The cells are being studied to be used as clinical therapies, models of genetic disorders, and cellular/DNA repair. (wikipedia.org)
This brings as oral dopamine therapies with the advantage that with it continued infective risks for the patients the cells can be targeted to the site where dopamine with DBS which is not so for patients with trans- is most needed, namely the striatum. (lu.se)

Cell Reports, Available online 14 November 2023. (lu.se)

All cell lines differentiated into three embryonic germ lineages in embryoid bodies and into neural cell lineages when cultured in neural differentiation medium. (biomedcentral.com)
We portrayed the Notch system in embryonic stem cell (ESC)-derived embryoid bodies (EBs) differentiating under the standard protocols used to assess yolk sac (YS) hematopoiesis in vitro. (karger.com)

This study describes for the first time, a differentiation system that does not require co-culture with animal cells or products, thus allowing the production of pathogen-free RPE cells suitable for transplantation into patients. (news-medical.net)
Summary of Research Progress: Our research aims to identify the optimal culture conditions and the best hESC lines for the derivation of nerve lineage cells in therapeutic cell transplantation. (ca.gov)
Finally, cell transplantation in a mouse stroke model will be used to study the mechanisms and efficacy of different types of hESC-derived neural cells in neural repair. (ca.gov)
Finally, we also made excellent progress on the procedure of cell transplantation. (ca.gov)
We have found a suitable substrate that can be used to enhance neuronal survival after cell transplantation and we expect to publish a research paper in this new method of cell transplantation. (ca.gov)
Transplantation of cells releasing γ‐aminobutyric acid (GABA) could be used to counteract the imbalance between ex-citation and inhibition within epileptic neuronal networks. (lu.se)
For decades, researchers in Penn's IRM have been pulling back the curtain on this process as they work toward developing new cells for transplantation and tissue repair as part of treatment for common problems like liver or heart disease. (newswise.com)
We monitor our cells after transplantation using standard in vivo assessments as listed above. (lu.se)
The primary outcome of the STEM-PD trial is to assess safety and tolerability of the transplanted product at 1-year post-transplantation, while secondary endpoints will assess survival and function of the transplanted cells by brain imaging, as well as measure effects on Parkinson's symptoms. (lu.se)

To complement the in vivo system of chick embryos, we employ patient omics data, single cell RNA sequencing, human and mouse embryos, human cancer cell cultures, human pluripotent stem cells, chick embryo neural crest-derived in vitro crestosphere cultures, and zebrafish and mouse in vivo models. (lu.se)

Why not use adult stem cells instead? (scitizen.com)
Adult Stem Cells: The New Plastic Surgery? (scitizen.com)
In the future, keratinocytes produced from pluripotent stem cells could be an alternative to adult stem cells in certain reconstructed tissue bio-engineering applications. (cea.fr)
One of the difficulties encountered in this area is that fact that the keratinocytes obtained do not have all the functions of adult stem cells. (cea.fr)
Despite the overwhelming success of adult stem cells for patients, few have heard the good news. (lifenews.com)
Adult Stem Cells have already shown published scientific evidence documenting effective treatment for spinal cord injury. (lifenews.com)
Laura Dominguez is one of the spinal cord injury patients who has benefited from adult stem cells. (lifenews.com)

An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. (thermofisher.com)
E-Cadherin is necessary for maintaining pluripotent stem cells and also for inducing the pluripotent state in the reprogramming of somatic cells," Dr. Besser said. (sciencedaily.com)

Awards will be used to create new technologies, tools, and/or processes that will help to study transient molecular complexes that are an integral part of normal cell physiology or that play a role in disease processes. (nih.gov)

Retinoblastoma proteins that inhibit the transcription factor E2F until the cell is ready to enter S phase are hyperphosphorylated and inactivated in ESCs, leading to continual expression of proliferation genes. (wikipedia.org)
A CEA-Jacob team has just published a paper in which it demonstrates the central role of the transcription factor KLF4 in regulating the proliferation of epidermal stem cells and their ability to regenerate this tissue. (cea.fr)
The study has shown that manipulation of KLF4 expression is also suitable for these cells, as reducing its expression in keratinocytes derived from embryonic stem cells (ESC) improves their proliferation capacity and their ability to reconstruct skin. (cea.fr)
Inhibits proliferation and promotes osteogenic differentiation, while inhibiting adipogenic differentiation, of human amnion-derived mesenchymal stem cells (MSCs) and rabbit bone marrow-derived MSCs (Wu et al. (stemcell.com)
Decreased proliferation and Nanog expression in mouse embryonic stem (ES) cells, with increased erythroid differentiation (Chae et al. (stemcell.com)
CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. (biolegend.com)
For several decades, neovascularization is believed to be accomplished by proliferation of mature endothelial cells residing in the local vessels through the process of angiogenesis [ 6 ]. (hindawi.com)

We studied the secretome profile of gestational tissue-derived MSCs and their effects on functions of endothelial progenitor cells (EPCs), another angiogenic cell type that plays an important role during the neovascularization. (hindawi.com)
However, recent studies have demonstrated that other angiogenic cells called endothelial progenitor cells (EPCs) also play an important role during the formation of new vessels through the process of vasculogenesis [ 7 - 9 ]. (hindawi.com)
Anderson, L. and Caldwell, M.A. (2007) Human neural progenitor cell transplants into the subthalamic nucleus lead to functional recovery in a rat model of Parkinson's disease. (scirp.org)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)

We disproved past misconceptions in the field about the role of Yap1 concerning its function in ES cell self-renewal, showing that like the inner cell mass, Yap1 is dispensable for long-term maintenance in culture. (utexas.edu)
Self-renewal conditions must prevent the cells from clumping and maintain an environment that supports an unspecialized state. (wikipedia.org)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)

ES cells have been an invaluable resource for advancing fundamental understanding of global transcriptional and epigenetic regulations, signaling pathways, and noncoding RNA in mammalian systems. (utexas.edu)
Differentiation is a complex process involving actions of ES cell core factors, lineage specific regulators, epigenetic modifications, and chromatin remodelers. (utexas.edu)
Epigenetic Analysis of Human Embryonic Stem Cells: Basics, Computational Methods, Applications. (mpg.de)
Multivariate statistical analysis of epigenetic regulation with application to the analysis of human embryonic stem cells. (mpg.de)
Using this type of direct conversion approach rather than making them from induced pluripotent stem cells, where they revert into a naïve state, helps to retain the epigenetic age of the patient in the generated interneurons. (lu.se)

This Funding Opportunity Announcement (FOA) solicits Small Business Technology Transfer (STTR) grant applications from small business concerns (SBCs) that propose to develop new technologies, tools, and/or processes for the study of transient molecular complexes. (nih.gov)
We show that the rtTA2s-S2 is useful for transient inducible expression of genes in embryonic stem cells. (muni.cz)
These cancers originate from cells of the sympathetic nervous system (SNS), in turn derived from the transient embryonic neural crest stem cell population. (lu.se)

Dr. Daniel Besser, Prof. Walter Birchmeier and Torben Redmer from the MDC, a member of the Helmholtz Association, used mouse embryonic fibroblasts (MEFs) in their stem cell experiments. (sciencedaily.com)
One way we did this was by converting adult fibroblasts, or skin cells, into interneurons. (lu.se)
Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)

However, the molecular mechanisms of how ES cells are differentiated remain much less understood. (utexas.edu)
Mitochondrial-associated cell death mechanisms are reset to an embryonic-like state in aged donor-derived iPS cells harboring chromosomal aberrations. (mpg.de)
However, until now, they've known little about the underlying molecular mechanisms responsible for these pathologies. (ucsf.edu)

The three PACE experiments executed 45-200 rounds of evolution in 1.5-8 days and yielded T7 RNAP variants with activities on their target promoters or templates that exceeded or matched the activity of the wild-type enzyme transcribing the wild-type T7 promoter both in cells and in vitro," the team states. (genengnews.com)
Parallel studies of a new human SOX17-eGFP ES cell line allowed analysis of endoderm differentiation in vitro, leading to culture modifications that enhanced expression of an endoderm-like signature. (uthscsa.edu)
At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
generated haploid male gametes from mouse embryonic stem cells that can produce viable and fertile offspring, demonstrating functional reproduction of meiosis in vitro. (scientificamerican.com)
This study explores the use of a simple human neurosphere-based in vitro model to characterise the pharmacological and toxicological effects of LiCl and VPA using gene expression changes linked to phenotypic alterations in cells. (plos.org)

Frontiers in Molecular Neuroscience. (lu.se)
Correspondence to: Roger A. Barker, Department of Clini- jections are lost, it does not tell us that this leads to cal Neuroscience and WT-MRC Cambridge Stem Cell Institute, the expression of many of the features of PD. (lu.se)

Transcription factor expression and the rate-limiting enzyme in dopamine synthesis tyrosine hydroxylase (TH) were detected in the transfected cells after 4 month-selection with G418, indicating transfected hONPs were stably restricted towards a dopaminergic lineage. (scirp.org)

Using whole‐cell patch‐clamp recordings, we characterised the maturation of the grafted cells into functional GABAergic inter-neurons in the host brain, and we confirmed the presence of functional inhibitory synaptic connections from grafted cells onto the host neurons. (lu.se)
The mice had new motor neurons, that were generated from embryonic stem cells, grafted onto the injured nerves. (medgadget.com)
The neurons were engineered to depolarize when illuminated via a light-sensitive ion channel that was genetically introduced into the cells. (medgadget.com)
We custom-tailored embryonic stem cells so that motor neurons derived from them can function as part of the muscle pacemaker device. (medgadget.com)
We then built a survival gene into them, which helps the stem-cell motor neurons to stay alive when they are transplanted inside the injured nerve and allows them to grow to connect to muscle. (medgadget.com)
However, with a growing number of novel cell sources (stem cells and reprogrammed cells) it becomes important to develop new methodologies for a more thorough assessment of human neurons generated using these approaches. (lu.se)
We utilise monosynaptic tracing technology, using pseudotyped rabies vectors, in order to visualise the synaptic contacts formed between host neurons and graft human cells. (lu.se)
The cells are called induced neurons (iNs) and offer a shortcut over induced pluripotent stem cells (iPSc) for generating patient and disease-specific neurons. (lu.se)
Current efforts focus on identifying the optimal starting cell population for neural conversion, identification of gene combinations that yield subtype specificity and analysis of function and integration of the reprogrammed neurons. (lu.se)
The use of stem cell-derived dopamine neurons or deep brain stimulation (DBS) represents two alternative approaches to treat Parkinson's Disease. (lu.se)

A biophysical and molecular characterization of human embryonic stem cell-derived exosomes (Master's thesis, University of Calgary, Calgary, Canada). (ucalgary.ca)

The first articles on the isolation of mouse ES cells were published in 1981 1 immediately heralding the possibility of isolating ES cells from other mammals. (scitizen.com)
Here, we describe purification of mouse DE using fluorescence-activated cell sorting (FACS) and mice harboring a transgene encoding enhanced green fluorescent protein (eGFP) inserted into the Sox17 locus, which is expressed in the embryonic endoderm. (uthscsa.edu)
Comparison of patterns of signaling pathway activation in native mouse DE and endoderm-like cells generated from ES cells produced novel culture modifications that generated Sox17-eGFP + progeny whose gene expression resembled DE more closely than achieved with standard methods. (uthscsa.edu)
These studies also produced new FACS methods for purifying DE from nontransgenic mice and mouse ES cell cultures. (uthscsa.edu)
However, we found that it was not possible to establish mouse embryonic stem cell lines stably expressing this transactivator. (muni.cz)
Carstea AC, Pirity MK, Dinnyes A. Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background. (wjgnet.com)
The team first made mouse PGCs, and then added cells taken from the testicular tissue of newborn mice, as well as other biological molecules. (scientificamerican.com)
The MDC researchers found that in contrast to the original cells, the new pluripotent cells derived from mouse connective tissue contained E-cadherin. (sciencedaily.com)
Here we use metabolic labelling with stable isotopes and mass spectrometry to quantitatively address the global turnover of genomic methylcytidine (mdC), hydroxymethylcytidine (hmdC) and formylcytidine (fdC) across mouse pluripotent cell states. (uni-muenchen.de)

Nat Rev Mol Cell Biol 1-18 (2019) doi:10.1038/s41580-019-0159-6. (uni-muenchen.de)

Human embryos reach the blastocyst stage 4-5 days post fertilization, at which time they consist of 50-150 cells. (wikipedia.org)
This new technique may help researchers avoid the controversies that come with the use of human embryos to produce human embryonic stem cells for research purposes. (sciencedaily.com)

Our signature-based screen established several categories of genes based on their comparative functions during the differentiation of ES cells. (utexas.edu)
PACE essentially comprises a system by which E. coli cells continuously flow through a fixed-volume vessel, or lagoon, which contains a replicating population of phage DNA vectors (the selection phage) encoding the gene or genes of interest. (genengnews.com)
The shape it takes has a profound influence on which genes in a cell are turned on or turned off. (umassmed.edu)
It's an intricate process guided by these so-called "pioneer" transcription factors that gain access to the tightly packed DNA inside each cell so other specialized proteins can get in and activate the necessary genes. (newswise.com)
They discovered that both nicotine and non-nicotine components of tobacco smoke increased the activity of genes that hold embryonic stem cells in a pluripotent, or undifferentiated, state. (ucsf.edu)
They determined that sentinel genes in each pathway were over expressed, but one most prominently: Expression of the Nodal gene was 50- to 75-fold higher in nicotine and tobacco smoke-exposed cells, respectively, than in untreated cells. (ucsf.edu)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)

Using immunocytochemistry, flow cytometry, qRT-PCR and MPSS, we found that all three cell lines actively proliferated and expressed similar "stemness" markers including transcription factors POU5F1/Oct3/4 and NANOG, glycolipids SSEA4 and TRA-1-81, and alkaline phosphatase activity. (biomedcentral.com)

Besides use in the aging retina, human embryonic stem cells have now demonstrated the potential to change the face of several age-related degenerative diseases, perhaps one day allowing physicians to prescribe young cells they way they dispense pills today. (news-medical.net)
Wet AMD occurs when abnormal blood vessels form underneath the macula - the section of the retina that allows detailed, central vision - and damage its cells. (medscape.com)
The pioneering treatment involved implanting an engineered patch of retinal pigment from epithelium cells under the retina to replace those that are damaged. (medscape.com)
1] Although this device will not restore vision to patients, it replaces the function of degenerated cells in the retina and may improve a patient's performance of basic activities by improving their ability to perceive images and movement. (medscape.com)

We also begin to produce and characterize a new type of human stem cells, namely induced pluripotent cells that are obtained by converting somatic cells into stem cell through reprogramming. (ca.gov)
The molecule also plays a crucial role in the reprogramming of somatic cells (body cells) into pluripotent stem cells. (sciencedaily.com)
In a second step the researchers investigated what happens when somatic cells that normally neither have E-cadherin nor are pluripotent are reprogrammed into a pluripotent stem cell state. (sciencedaily.com)
In this reprogramming technique, somatic cells are converted into induced pluripotent stem cells (iPSCs). (sciencedaily.com)
If E-cadherin is absent, somatic cells cannot be reprogrammed into viable pluripotent cells. (sciencedaily.com)
Understanding the molecular relationships is essential for using human somatic cells to develop stem cell therapy for diseases such as heart attack, Alzheimer's or Parkinson's disease or diabetes," Dr. Besser said. (sciencedaily.com)

2021. Cancer Cell. (biolegend.com)
2021. Cell Reports. (biolegend.com)
The STEM-PD trial is investigating the safety and tolerability of transplanting STEM-PD cells into the brain of patients with moderate Parkinson's disease (EudraCT Number:2021-001366-38). (lu.se)

Epigenomic analysis of primary human T cells reveals enhancers associated with TH2 memory cell differentiation and asthma susceptibility. (mpg.de)

Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)

Researchers are currently focusing heavily on the therapeutic potential of embryonic stem cells, with clinical use being the goal for many laboratories. (wikipedia.org)

Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. (mdpi.com)

Ethical approval of the trial has already been obtained from the Swedish Ethics Review Authority, and the STEM-PD team, led from Lund University in Sweden, is thereby ready to proceed with the trial. (lu.se)

Comparative molecular portraits of human unfertilized oocytes and primordial germ cells at 10 weeks of gestation. (mpg.de)

Millions of patients with retinal degeneration might conceivably benefit from these cells in the future," said Robert Lanza, Medical Director at ACT, and senior author of the study. (news-medical.net)
Commenting on the trial in a statement, Dr Carmel Toomes, associate professor at the Leeds Institutes of Molecular Medicine, says: "These results give the many patients out there who suffer from AMD and other retinal degenerations real hope that stem cells replacement therapy may be a reality in the near future. (medscape.com)

These cells have been in high demand for use in basic and applied biomedical research. (biomedcentral.com)
The comparison of the unique properties and behavior of each individually derived cell line is critical in identifying the safe and efficacious lines for research and therapeutic use [ 3 , 13 ]. (biomedcentral.com)
The real value of over two decades of ES-cell research currently lies with these advances but there has been relatively little external acclaim or general interest. (scitizen.com)
These debates will, and must, rage on but it interesting that two things in particular seem to be lost to most debates on the issue: (i) the value of research to this point in time, and (ii) the likelihood that the use of stem cells of any type to treat most if not all these diseases remains, for a variety of reasons, a distant possibility. (scitizen.com)
The discoveries of a French research team from the CEA, INSERM and the University of Paris, produced in collaboration with I-Stem, the AFM-Téléthon laboratory, and the University of Évry, opens perspectives for regenerative cutaneous medicine, in particular for the bio-engineering of skin grafts for tissue reconstruction. (cea.fr)
My research continues to focus on stem cells and osteoblasts and in particular 3D cell culture models and microenvironments. (nottingham.ac.uk)
My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
We know second-hand smoke exposure is bad for the developing fetus, causing everything from heart defects to childhood cancer, but we haven't understood why," said senior author Harold S. Bernstein , MD, PhD, who is a UCSF professor of pediatrics and a member of the Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research at UCSF. (ucsf.edu)
The danger has made even some proponents of embryonic stem cell research concerned about the risky nature of Geron s human experiment , instead of making a political point . (lifenews.com)
Receive a free daily email report from LifeNews.com with the latest pro-life news stories on abortion, euthanasia and stem cell research. (lifenews.com)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
Great Iranian Muslim scholars netics, stem cell research, and organ trans- laid huge emphasis on teaching and practis- plantation are some of the medical issues ing ethics. (who.int)
Cell research 2020 6 0. (cdc.gov)
Andreas' research focuses on generating brain cells, specifically interneurons, in the lab, which has significant implications for the study of neurodegenerative and neuropsychiatric disorders. (lu.se)

Next, they exposed human embryonic stem cells in the culture dish either to tobacco smoke or nicotine at concentrations found in fetal blood. (ucsf.edu)

Consistent with previous observations of repressive role of Rbpj, Rbpj serves as a repressor of ES cell core factors in the absence of Notch signaling pathway. (utexas.edu)

In tissue engineering, the use of stem cells are known to be of importance. (wikipedia.org)
Fig. 1: Design of the MiSTR cell culture system and establishment of a WNT signaling gradient in hESC-derived tissue. (nature.com)
Human skin completely renews itself every month thanks to the presence of stem cells in the deepest layer, which generate all the upper layers of this tissue. (cea.fr)
I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
Previous studies demonstrated that MSCs could ameliorate the pathology associated with ischemic heart disease, ischemic stroke, and peripheral vascular disease by releasing several critical factors that increase cell survival, provide an appropriate microenvironment for repairing damaged tissue, and induce neovascularization [ 3 - 5 ]. (hindawi.com)
Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)

3. Centre for the Study of Stem Cells (CECS), Institute for Stem Cell Therapy and Exploration of Monogenic Diseases (I Stem), 91100 Corbeil Essonne, France. (cea.fr)
En 1990, il est recruté par le Salk Institute de San Diego pour lancer un centre de recherche sur le SIDA. (epfl.ch)

The focus has been on GABAergic interneurons and making them from different starting cells. (lu.se)
I've also worked with differentiation from embryonic stem cells into interneurons. (lu.se)

The non-invasive in situ MPM imaging analysis in this study is valuable because it enables longitudinal, 3D analysis of the structural and functional status of thick, opaque scaffolds as they interact with stem cells and are being degraded. (rsc.org)
The results of the paper published in Nature Biomedical Engineering show that reducing the expression of the KLF4 gene during graft preparation promotes rapid expansion of functional stem cells1, without damaging their genomic stability. (cea.fr)
1. A single functional stem cell is able to regenerate the skin throughout a person's life. (cea.fr)
Here, we focus on functional homologies by performing a comparison between the circuitry connecting these players in plants and animals and find striking similarities, suggesting that comparable regulatory logics have been evolved for stem cell regulation in both kingdoms. (lu.se)
This includes long-term survival of transplanted cells in vivo to assess cell fate, maturation and integration, as well as functional behavioural assessment for graft function in vivo. (lu.se)

However, a profound variation in colony morphology, growth rate, BrdU incorporation, and relative abundance of gene expression in undifferentiated and differentiated states of the cell lines was observed. (biomedcentral.com)
Using chromosome conformation capture technologies in conjunction with advanced computational modeling and a range of imaging methods, the center will generate three-dimensional models of the human genome inside fibroblast cells during metaphase and interphase, as well as in embryonic stem cells in undifferentiated and differentiated states. (umassmed.edu)

Single-cell transcriptomics revealed that rostro-caudal organization was already established at 24 h of differentiation, and that the first markers of a neural-specific transcription program emerged in the rostral cells at 48 h. (nature.com)
Transcription factor RUNX1 promotes survival of acute myeloid leukemia cells. (medlineplus.gov)

The effi- risk of infection - DBS requires battery changes cacy of dopaminergic drug treatment provides a com- with a clear physical and somewhat unsightly pelling rationale for the use of dopamine cell thera- reminder of an intervention, as is evident by pies to restore striatal dopamine neurotransmission. (lu.se)

Anatomy51

Organisms11

Diseases5

Chemicals and Drugs71

Analytical, Diagnostic and Therapeutic Techniques and Equipment55

Psychiatry and Psychology1

Phenomena and Processes62

Disciplines and Occupations5

Technology, Industry, Agriculture1

Humanities1

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Early embryonic development5

Biology15

Regenerative medicine9

Harvested from human embryonic stem1

Differentiation of human embryonic stem2

HESCs7

20222

Genetics2

Normal embryonic development2

Impaired embryonic development1

Oct47

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Differentiate11

Abstract1

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20231

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Human pluripotent stem1

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Physiology1

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20213

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Undifferentiated and differentiated2

Transcription2

Dopamine cell1