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Electrophoresis, Gel, Two-DimensionalElectrophoresis, Polyacrylamide GelElectrophoresis, Agar GelComputersMolecular WeightGelsProteomicsMolecular Sequence DataAmino Acid SequenceBacterial ProteinsElectrophoresisElectrophoresis, CapillaryElectrophoresis, Gel, Pulsed-FieldVisual FieldsElectrophoresis, MicrochipElectromagnetic FieldsElectrophoresis, DiscElectrophoresis, Starch GelBlood Protein ElectrophoresisDenaturing Gradient Gel ElectrophoresisElectrophoresis, Cellulose AcetateIsoelectric FocusingMagnetic FieldsDNA, BacterialKineticsBase SequenceChromatography, GelAmino AcidsBacterial Typing TechniquesPolymerase Chain ReactionHydrogen-Ion ConcentrationVisual Field TestsIsoelectric PointDNA FingerprintingEscherichia coliChromatography, AffinityImmunoelectrophoresisChromatography, Ion ExchangeSodium Dodecyl SulfateElectrophoresis, PaperProteomeDNASubstrate SpecificitySpecies SpecificityCattleRabbitsChromatography, DEAE-CelluloseProteinsTemperatureSequence Analysis, DNAImmunodiffusionMacromolecular SubstancesCentrifugation, Density GradientTwo-Dimensional Difference Gel ElectrophoresisTrypsinChromatographyChromatography, High Pressure LiquidSerotypingSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationMass SpectrometryUltracentrifugationPeptidesCloning, MolecularCell LinePeptide FragmentsCapillary ActionHot TemperaturePlasmidsMicroscopy, ElectronChemical PrecipitationLiverMutationTime FactorsDeoxyribonucleases, Type II Site-SpecificRNA, Ribosomal, 16SDensitometryPolymorphism, Restriction Fragment LengthSwineGlycoproteinsPhylogenyCarbohydratesIsoenzymesNucleic Acid DenaturationProtein BindingBlood ProteinsCell MembraneGenotypeReproducibility of ResultsMembrane ProteinsMolecular EpidemiologyGenetic VariationChemistrySensitivity and SpecificityImmunoelectrophoresis, Two-DimensionalDisease OutbreaksGenes, BacterialChemical PhenomenaDNA, RibosomalDNA PrimersCell Fractionation
PFGEIsolatesEpidemiologyAgarose gel electrophorMicrobiologyEpidemiologicalStrainsMethodsPolymeraseAntimicrobialCharacterizationAssaysQuantitativeAnalysisPulseNetSalmonellaBacteriaGenomeGeneticsLarge DNA moleculesLaboratoryMethodRestrictionFragmentsApproachesStudySearchDataTechniquePatternIdentifyWeightApplicationsTimeConditions
PFGE28
  • Pulsed-field gel electrophoresis (PFGE) is a method used to separate large segments of DNA using an alternating and cross field. (wikipedia.org)
  • By the late 1980s and early 1990s, PFGE had become a widely used technique for the separation and analysis of large DNA molecules in various fields of research, including molecular biology, genetics, and biotechnology. (wikipedia.org)
  • Pulsed-field gel electrophoresis (PFGE) is performed to subtype isolates of foodborne pathogens such as Salmonella sp. (in.gov)
  • Pulsed-field gel electrophoresis, or PFGE, is a DNA-based typing technique that is highly discriminatory and has been used in epidemiological investigations of S. zooepidemicus outbreaks. (cdc.gov)
  • Altogether 156 P. larvae isolates from honey or brood samples supplied by 114 beekeepers were analysed by Not I pulsed-field gel electrophoresis (PFGE) and biotyping, as well as for tetracycline resistance. (apidologie.org)
  • Pulsed-field gel electrophoresis (PFGE) is a highly discriminative molecular typing technique that is used in epidemiological studies worldwide. (applied-maths.com)
  • PFGE is based upon the variable migration of large DNA restriction fragments in an electrical field of alternating polarity. (applied-maths.com)
  • Also various molecular typing techniques have been used, including pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), and these assays were considered as efficient techniques in MRSA typing but they are time consuming, expensive, and technically complex [ 8 ]. (hindawi.com)
  • Agarose MP, a multi purpose agarose, is developed for analytical and preparative electrophoresis of nucleic acids and separation of high molecular weight DNA via pulse field gel electrophoresis (PFGE).It is also used for preparative electrophoresis and quantitative recovery of biologically active DNA. (sigmaaldrich.com)
  • NEB also offers various conventional double-stranded DNA molecular weight markers with sizes up to 23 kb and markers for pulsed-field gel electrophoresis (PFGE) ranging from 1.5 to 1,018 kb. (neb.com)
  • The Campylobacter isolated from the four lab-confirmed cases are a match by molecular techniques (pulsed-field gel electrophoresis, or PFGE). (foodpoisonjournal.com)
  • Subtyping of outbreak-associated isolates using pulsed-field gel electrophoresis (PFGE) revealed two similar but distinct Asc I PFGE patterns. (biomedcentral.com)
  • PFGE has been adopted by PulseNet as the internationally standardized method for molecular subtyping of L . monocytogenes and has been essential in the detection and investigation of listeriosis outbreaks in Canada and worldwide [ 18 - 20 ]. (biomedcentral.com)
  • Public health investigators used DNA "fingerprints" of Salmonella bacteria obtained through diagnostic testing with pulsed-field gel electrophoresis, or PFGE, to identify cases of illness that may be part of this outbreak. (cdc.gov)
  • Electrophoretic karyotyping is a relatively new experimental technique whereby the chromosomes of lower eukaryotes are physically separated in an agarose gel matrix by pulsed field gel electrophoresis (PFGE), stained with ethidium bromide, and visualized when irradiated with ultraviolet light. (taylorfrancis.com)
  • This deletion is molecularly defined by the identification of a patient specific junction fragment by Southern analysis following pulsed field gel electrophoresis (PFGE). (bmj.com)
  • In December 2009 the Minnesota Department of Health (MDH) uploaded results of molecular testing by Pulsed Field Gel Electrophoresis (PFGE) of an E. coli O157:H7 isolate cultured from a patient stool specimen to PulseNet, a national database of molecular subtyping or "fingerprinting" of foodborne disease causing bacteria. (marlerblog.com)
  • Pulsed Field Gel Electrophoresis (PFGE) was used to assess the genomic features of the bacterial isolates. (biomedcentral.com)
  • Molecular approaches such as PFGE that compare the DNA differences of bacteria have been accepted because of establishing the clonal association in many bacteria including A. baumannii isolates [ 10 ]. (biomedcentral.com)
  • MMWR reported today: In August 2014, PulseNet, the national molecular subtyping network for foodborne disease surveillance, detected a multistate cluster of Salmonella enterica serotype Newport infections with an indistinguishable pulse-field gel electrophoresis (PFGE) pattern (XbaI PFGE pattern JJPX01.0061). (justia.com)
  • In this study, the characteristics of A. otitidis isolates from patients with OME were investigated via Pulsed-Field Gel Electrophoresis (PFGE) typing method. (ac.ir)
  • The molecular characteristic of A. otitidis was investigated by PFGE technique. (ac.ir)
  • Conclusion: The current study, being the first reports on the molecular typing of A. otitidis by PFGE method, shows that A. otitidis is a heterogenic organism in Iranian children who have OME. (ac.ir)
  • The surveillance and study of outbreaks of the FBD through pulsed field gel electrophoresis (PFGE) is a fundamental support for epidemiological research. (bvsalud.org)
  • New methods based on the genotypic properties have been developed and used for the proper classification of bacteria The aim of this research was the isolation of lactic acid bacteria from raw milk and the identification of the lactic acid bacterial isolates by biochemical tests, polymerase chain reaction (PCR)-based methods and pulsed field gel electrophoresis (PFGE). (iyte.edu.tr)
  • Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were used for molecular typing. (hal.science)
  • Although molecular typing identified 126 E. faecalis pulsed-field gel electrophoresis (PFGE) pulsotypes, more than 50% belonged to 2 pulsotypes that were isolated across Australia. (edu.au)
  • Ohio increased the capacity for conducting DNA fingerprinting using pulsed-field gel electrophoresis (PFGE). (cdc.gov)
Isolates10
  • Pulsed-field gel electrophoresis patterns and similarity dendrogram of genomic DNA from Aeromonas hydrophila and A. caviae isolates from diarrheic stool (S) and groundwater (W). The number refers to the isolate number. (cdc.gov)
  • The isolates were analyzed using pulsed-field gel electrophoresis, multilocus sequence typing and sequencing of the szP gene. (cdc.gov)
  • The molecular typing methods showed that human and equine isolates were identical or closely related. (cdc.gov)
  • That the number of chromosomes among field isolates of other fungal pathogens is variable is suggestive that aneuploidy may also play a significant role in karyotype variability. (taylorfrancis.com)
  • The purpose of this study was to identify antibiotic resistance patterns, biofilm formation and the clonal relationship of clinical and environmental isolates of A. baumannii by Pulsed Field Gel Electrophoresis method. (biomedcentral.com)
  • BKC-1-positive isolates were typed using pulsed-field gel electrophoresis and multi-locus sequence typing. (cardiff.ac.uk)
  • In this study, we aimed to investigate the epidemiology and molecular characteristics of S. dysenteriae isolates from cattle farms in Gansu, China. (researchsquare.com)
  • Our analysis includes the antimicrobial resistance profiles, the molecular characterization of the mechanisms of resistance to fluoroquinolones, virulence gene profiles and the molecular characterization of S. dysenteriae isolates by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). (researchsquare.com)
  • The aim of AESOP 2011 was to determine the proportion of enterococcal bacteraemia isolates in Australia that are antimicrobial resistant, with particular emphasis on susceptibility to ampicillin and the glycopeptides, and to characterise the molecular epidemiology of the Enterococcus faecalis and E. faecium isolates. (edu.au)
  • Pulsed-field gel electrophoresis patterns for 11 isolates were not compatible with known USA types or clones. (elsevierpure.com)
Epidemiology2
  • This study confirms that the latest generation of DNA sequencing technologies can be applied during high priority public health events, and laboratories need to prepare for this inevitability and assess how to properly analyze and interpret whole genome sequences in the context of molecular epidemiology. (biomedcentral.com)
  • Objectives: To investigate the molecular epidemiology of Acinetobacter baumannii strains isolated from different hospitals in Lebanon. (hal.science)
Agarose gel electrophor1
Microbiology1
  • Important progress made in the fields of instruments, reagents and techniques have make it easier to adapt to the important changes of the clinical microbiology context e.g. increasing use of microbiology tests, shortage of qualified personnel. (isid.org)
Epidemiological1
  • BIONUMERICS uses industry leading database engines that allow you to store all your epidemiological info and gel images in one database. (applied-maths.com)
Strains5
  • These testing results were provided to the CDC, which compared the molecular fingerprint data from kratom samples to the CDC's PulseNet database of salmonella fingerprint data of the salmonella strains collected from sick people in the United States. (foodsafetynews.com)
  • Molecular determinants enabling the imipenem resistant strains to exhibit co-resistance to aminoglycosides and fluoroquinolones from this geographical region were delineated. (biomedcentral.com)
  • Pulsed field gel electrophoresis was also optimized for the identification of reference strains. (iyte.edu.tr)
  • To better understand the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) colonization or infection in different patient populations, to perform quantitative analysis of MRSA in nasal cultures, and to characterize strains using molecular fingerprinting. (elsevierpure.com)
  • MRSA strains were quantified and characterized by molecular fingerprinting. (elsevierpure.com)
Methods4
  • The Molecular Test Development Laboratory develops and implements methods for detection and subtyping of various gastrointestinal and respiratory pathogens. (in.gov)
  • Methods in molecular biology , pp.265-275, 2015, ⟨10.1007/978-1-4939-2599-5_21⟩ . (hal.science)
  • In Methods in Molecular Biology (pp. 33-41). (ncsu.edu)
  • In Methods in Molecular Biology (pp. 163-174). (ncsu.edu)
Polymerase1
Antimicrobial1
Characterization1
  • This study aimed to investigate the prevalence of fluoroquinolone resistance and associated molecular characterization of S. dysenteriae 1 isolated from calves. (researchsquare.com)
Assays1
  • Spartan Bioscience has unveiled the first assays for its molecular diagnostics device and promised further launches in food and water safety testing. (foodnavigator.com)
Quantitative2
  • The rate of DNA breakage is then measured under an inverted and asymmetric electric field, allowing for quantitative measurement. (wikipedia.org)
  • Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry. (electroforese.com)
Analysis3
  • Molecular analysis of Leptospira spp. (degruyter.com)
  • 2007). Molecular karyotype analysis of Perkinsus atlanticus (Phylum Perkinsozoa) by pulsed field gel electrophoresis . (up.pt)
  • Agarose MP is particularly well suited for pulsed-field gel electrophoresis, because it allows short separation times for large DNA molecules, while maintaining the high resolution required for effective analysis. (sigmaaldrich.com)
PulseNet1
  • They used data from PulseNet , the national subtyping network made up of state and local public health laboratories and federal food regulatory laboratories that performs molecular surveillance of foodborne infections. (cdc.gov)
Salmonella1
  • The illnesses were linked to each other through molecular fingerprinting of the Salmonella I 4,[5],12:b:- bacteria using a laboratory technique called pulsed-field gel electrophoresis. (foodsafetynews.com)
Bacteria1
  • has been identified as the 2nd most consensus (ERIC) sequences which · 60 environmental samples were tak- frequent organism causing ventilator- are common to Gram-negative enteric en throughout the ICU, including associated pneumonia, the 4th most bacteria [11,12]. (who.int)
Genome1
  • This can cause variations in the migration of DNA throughout the gel, making it challenging to distinguish and map the different components of the genome, particularly in mammalian cells. (wikipedia.org)
Genetics2
  • Dr Ried received a Masters Degree in Chemistry (1993) and PhD in Human Molecular Genetics from the University of Heidelberg, Germany (1997). (niim.com.au)
  • Her PhD and early postdoctoral work in human genetics at the Centre of Molecular Genetics, University of Adelaide, culminated in the discovery of various genes, one of which is involved in the development of cancer. (niim.com.au)
Large DNA molecules2
  • Pulsed field gel electrophoresis is a technique used for the separation of large DNA molecules by applying to a gel matrix an electric field that periodically changes direction. (wikipedia.org)
  • The first reference to the use of the term "pulsed-field gel electrophoresis" appears in a 1983 paper by Dr. Richard L. Sweeley and colleagues at the DuPont Company, where they described a method of separating large DNA molecules (over 50 kb) by applying a series of alternating electric fields to a gel matrix. (wikipedia.org)
Laboratory1
  • A Listeria monocytogenes typing assessment has shown the majority of labs were able to produce gels of sufficient quality for inter-laboratory comparisons but the general quality needs improvement. (foodnavigator.com)
Method2
Restriction2
Fragments2
  • OFAGE uses non-uniform electric fields to separate large DNA fragments. (wikipedia.org)
  • DNA fragments are separated during electrophoresis using an electrical field of alternating polarity, after which the fingerprints are visualized and documented. (applied-maths.com)
Approaches2
  • Fluorescent Protein Technology - It took over thirty years, and the advent of recombinant DNA as well as vastly improved molecular biological approaches to see the pioneering work of Osamu Shimomura developed into a useful tool for live-cell imaging by Doug Prasher and Martin Chalfie. (fsu.edu)
  • Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches. (electroforese.com)
Study2
  • According to the literature review, there is no study about the molecular typing of A. otitidis. (ac.ir)
  • Carolina Duarte participated in all the stages of the un hospital pediátrico de tercer nivel en Colombia - 2016 study. (bvsalud.org)
Search1
  • Select up to three search categories and corresponding keywords using the fields to the right. (cdc.gov)
Data1
  • This important feature makes it possible for labs to exchange and compare electrophoresis data obtained with different conditions or setups. (applied-maths.com)
Technique1
  • This is a technique that separates large segments of DNA by periodically flipping two fields at a straight angle. (wikipedia.org)
Pattern1
  • In a uniform magnetic field, components larger than 50kb move through the gel in a zigzag pattern, allowing for more effective separation of DNA molecules. (wikipedia.org)
Identify1
  • Using a combination of next-generation sequencing, bioinformatics, and molecular cloning, CDC can identify the functions of the individual antibodies and what part of the influenza virus they target. (cdc.gov)
Weight1
Applications1
  • AsureQuality and Ubiquitome are to collaborate on trialling mobile molecular testing for applications in food and primary production sectors. (foodnavigator.com)
Time1
  • The movement of DNA molecules is facilitated by shorter time pulses or less force in the reverse direction. (wikipedia.org)
Conditions1