• Aptamer developers benefit from our extensive experience in aptamer characterization and our derived broad portfolio of suitable biophysical assays. (2bind.com)
  • Wang began working in the pharmaceutical industry in 2015, when he joined Janssen to develop biochemical and biophysical assays, which are fundamental tools in early-stage drug discovery. (acs.org)
  • Development of an Analytical Assay for Electrochemical Detection and Quantification of Protein-Bound 3-Nitrotyrosine in Biological. (aidstar-one.com)
  • Typically, site-directed and random mutagenesis are used to elucidate biological processes, determine protein structure, and develop screening assays, research reagents, and protein drugs. (codondevices.com)
  • In addition, we gained new insights into the structure and function of this protein through molecular dynamics simulations. (bvsalud.org)
  • We identified new conformational states of the protein and determined that residues Phe367, Tyr368, and Gln354 within the binding pocket serve as stabilizing residues for novel ligand interactions. (bvsalud.org)
  • Importantly, the KMET-READ project will also develop a yeast-3-hybrid method for the identification of new reading domains, which will allow to discover binding partners for just recently characterized new protein methylation marks. (europa.eu)
  • It has some strange multiple mutations in the spike protein, namely, N501Y and A570D in the receptor-binding domain (RBD) and the N terminal domain, respectively. (genscript.com)
  • SARS-CoV-2 Spike protein (S1, A570D, His Tag) can bind with human ACE2 (Cat. (genscript.com)
  • The interaction of sGAG with α2-macroglobulin receptor-associated protein (LRPAP1), exportin-1 (XPO1), and serine protease HTRA1 (HTRA1) was confirmed in reverse assays. (degruyter.com)
  • To test this hypothesis, we carried out ChIP assays treating the MCF-7/CD44 cells with formaldehyde (a protein-DNA crosslinker) as well as with DSG (a protein-protein crosslinker). (emerging-researchers.org)
  • We have concluded that the crosslinking process in the ChIP assay might be affecting the availability of the CD44-ICD epitope since in Proximity Ligation Assays (PLA) in which no crosslinkers are used, we were able to detect the hypothesized CD44-ICD/Runx2 protein-protein interaction in the same cell line using the same anti-CD44-ICD antibody. (emerging-researchers.org)
  • Analyze up to 10 000s of small molecule compounds or molecular fragments for binding to a protein target. (2bind.com)
  • Rapid and sensitive biomarkers and bioassays, both in vitro (e.g. protein binding assays, enzyme induction assays) and in vivo (e.g. zebrafish embryo assay) methods to measure the exposure, bioavailability, and effects of chemicals with endocrine disrupting potency are available. (vu.nl)
  • Binding affinity is validated by ELISA with recombinant soluble protein antigen. (genemedi.net)
  • Protein digestion coupled to liquid chromatography and tandem mass spectrometry (LC-MS/MS) detection enables multiplexed quantification of proteins in complex biological matrices. (cdc.gov)
  • However, although Michael acceptors are reactive, the Michael addition is reversible and it can be assumed that also other parts of the molecules will moderate the binding to and the release from any given nucleophilic site in a protein, and thereby moderate a specific biological activity. (lu.se)
  • Goethe-Universita¨t, DE-60439 tein (I-LBP) and intestinal fatty acid-binding protein (I-FABP). (lu.se)
  • The H5N1 Inventory is an inclusive compilation of amino acid changes and/or motifs identified within each viral protein that affect one or more biological properties, provided in broad categories. (cdc.gov)
  • The proposal will apply an interdisciplinary approach in an international environment to maximize its chances of success: the biological role of reading domains will be evaluated with molecular biology (histone and chromatin pulldowns, ChIP-seq, confocal microscopy), biochemistry (Peptide arrays, mass spectrometry, methyltransferase activity assays) and biophysics techniques (fluorescence anisotropy, circular dichroism spectroscopy) as well as crystallography (solving the structure of reading domains). (europa.eu)
  • With the advancement of next-generation sequencing and mass spectrometry, there is a growing need for the ability to merge biological features in order to study a system as a whole. (rsc.org)
  • Rather, mass photometry, crosslinking mass spectrometry, single molecule TIRF microscopy and biochemical assays identify inter-holoenzyme phosphorylation (IHP) as the mechanism for spreading phosphorylation. (elifesciences.org)
  • Support from the Swedish National Infrastructure for Biological Mass Spectrometry (BioMS) and the SciLifeLab, Integrated Structural Biology (ISB) platform, is gratefully acknowledged. (lu.se)
  • To characterize the enzymatic activity of SARS-CoV-2 M pro and to carry out high-throughput screening of inhibitors, we developed a fluorescence resonance energy transfer assay. (nature.com)
  • Both enantiomers of the esterified form of the compound, as well as its corresponding carboxylic acids, were found active in fluorescence polarization (FP) assay, nuclear magnetic resonance (NMR) and microscale thermophoresis (MST) assay, with K i and K D values around 1 μm. (rcsb.org)
  • This course is on advanced fluorescence microscopy imaging and spectroscopy techniques for quantitative characterization of molecular transport and interactions in cells. (ki.se)
  • 3. Describe the theoretical background behind specialized fluorescence based methodologies for studying molecular interactions in live cells. (ki.se)
  • 4. Specify instrumental requirements and design a fluorescence imaging assay for a biological problem of interest. (ki.se)
  • 5. Apply a specific labeling strategy and perform a fluorescence imaging assay. (ki.se)
  • Fluorescence microscopy and associated techniques are indispensable research tools for investigating molecular mechanisms of biological processes. (ki.se)
  • Through a combination of structure-based virtual and high-throughput screening, we assayed more than 10,000 compounds-including approved drugs, drug candidates in clinical trials and other pharmacologically active compounds-as inhibitors of M pro . (nature.com)
  • One of these compounds (ebselen) also exhibited promising antiviral activity in cell-based assays. (nature.com)
  • Co-crystallization studies show that these compounds bind similarly to FA, but deeper within the PA pocket. (bvsalud.org)
  • Regarding the biological results of the chromone-based compounds, similar structure-activity correlations could be drawn from the binding assay and the whole cell activity testing on M. tuberculosis. (uni-wuerzburg.de)
  • Molecular imprinting is a technology that facilitates the production of artificial receptors toward compounds of interest. (mdpi.com)
  • These tests screen libraries of compounds to find any that affect the activity of biological targets, such as enzymes, that may be involved in a disease. (acs.org)
  • A traditional assay would look for compounds that bind to an activated kinase. (acs.org)
  • In silico molecular modeling methods, such as docking can aid in the drug discovery process by ascertaining the binding affinities of existing and hypothetical compounds towards Pf TrxR and the human isoform of this enzyme. (biomedcentral.com)
  • The IC50 values in the two cell lines were found to depend on the overall structure of the assayed compounds, although less in this subset of compounds compared to a previous investigation. (lu.se)
  • Engineer therapeutic proteins to improve properties such as stability, potency, binding affinity, and immunogenicity. (codondevices.com)
  • Notably, we discovered NSC620333, a compound that exhibits a strong binding affinity to nsp14 with a dissociation constant of 427 ± 84 nM. (bvsalud.org)
  • These three variants share the N501Y substitution in the receptor-binding domain (RBD) of spike glycoprotein (S), which increases the binding affinity of S with the virus's cellular receptor, angiotensin-converting enzyme 2 (ACE2) 2 (see Fig. 1 ). (nature.com)
  • These cutting-edge biophysical tools are revolutionizing our understanding of molecular-level processes because they reveal an entire binding profile between unlabeled reactants and dissect it into kinetic components from which an affinity can be deduced. (intechopen.com)
  • Leveraging the sophisticated capabilities of GM's TaurusTM and LIBRATM platforms, GMab synthesizes antibodies characterized by high binding affinity, exceptional physicochemical stability, and optimal developability profiles. (genemedi.net)
  • IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. (rndsystems.com)
  • Lysine methylation marks manifest their biological effect via so-called 'readers' (or reading domains) which recognize and bind the methylation mark and directly alter the chromatin structure or act as a scaffold for other proteins, which induce biological responses. (europa.eu)
  • By identifying many sGAG-specific interacting proteins, our data provide a resource for upcoming studies aimed at molecular mechanisms and understanding of sGAG cellular effects. (degruyter.com)
  • Glycosaminoglycans (GAGs) are multifunctional polysaccharides of the extracellular matrix (ECM) responsible for ECM hydration and binding of cations and proteins due to their negative charge. (degruyter.com)
  • The biological system is complex with many regulatory features such as DNA, mRNA, proteins, metabolites, and epigenetic features such as DNA methylation and histone post-translational modifications (PTMs). (rsc.org)
  • In AIM2, we will identify bona fide RG4-binding proteins using a novel approach based on proteomic/biochemical analysis of interactions between the 7-deazaguanine RNA derivatives and proposed binding factors. (grantome.com)
  • Most of the circulating testosterone is bound to carrier proteins (SHBG = sex hormone-binding globulin). (cdc.gov)
  • Some authors propose that serum thyroid hormone abnormalities are due to inhibition of thyroid hormone binding to proteins, thus preventing tests from appropriately reflecting free hormone levels. (medscape.com)
  • However, the reproducibility of enzymatic digestion of proteins to produce proteotypic target peptides is a major limiting factor of assay precision. (cdc.gov)
  • Hydration of Lipid-binding Proteins by ten anti-parallel b-strands. (lu.se)
  • These luciferase expressing cells can be used as a target cancer cell for in vitro killing assay by CD20 CAR-T cells. (atcc.org)
  • Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. (rndsystems.com)
  • Using a multidisciplinary approach, we examined the mechanism of binding of compound 5a to the nsp16 cryptic pocket and developed 5a derivatives that inhibited nsp16 activity and murine hepatitis virus replication in rat lung epithelial cells but proved cytotoxic to cell lines canonically used to examine SARS-CoV-2 infection. (bvsalud.org)
  • Watch our on-demand webinar to learn how immortalized primary cells can solve the problem of limited biological relevancy in cell-based assays. (atcc.org)
  • ATCC (American Type Culture Collection) is a nonprofit, global biological resource center and standards organization and the leading developer and supplier of authenticated cells lines and microorganisms. (atcc.org)
  • The assay is based on immature rat granulosa cells producing plasminogen activator in response to hFSH. (ucl.ac.uk)
  • Systems toxicology, therefore, aims at understanding and exploring the way that different biological components are orchestrated as an ensemble in cells, tissues, and organisms. (brill.com)
  • Determined by a cell proliferation assay using Balb/c 3T3 cells. (peprotech.com)
  • We thank Professor Ramanujan Hegde (MRC Laboratory of Molecular Biology, Cambridge, UK) for the kind gift of anti‐TRAPα antibody and Professor Peter Arvan (University of Michigan Medical School, Ann Arbor, MI, USA) for the kind gift of INS‐1 832/13 cells. (lu.se)
  • This binding inhibitor can be present both in the serum and in body tissues and might inhibit uptake of thyroid hormones by cells or prevent binding to nuclear T3 receptors, thus inhibiting the action of the hormone. (medscape.com)
  • As a follow-up of a previous investigation in which semisynthetic damsin derivatives were shown to possess up to 10 times higher cytoxicity in JIMT-1 breast cancer cells compared to normal breast epithelial MCF-10A cells, a range of new derivatives were prepared and assayed toward the same cells. (lu.se)
  • Selected antibody fragments were characterized using biophysical and biological assays. (open.ac.uk)
  • We characterized several antibody fragments, which exert their inhibitory effect of VEGFR-2 independent of ligand binding. (open.ac.uk)
  • Endogenous testosterone released from the sample by ANS (8-anilino-1-naphthalene sulfonic acid) and norgestrel competes with the added testosterone derivative labeled with ruthenium complex for the binding sites on the biotinylated antibody. (cdc.gov)
  • The binding sites of the labeled antibody become occupied partly by the sample analyte (depending on its concentration) and partly by the ruthenium-labeled hapten to form the respective immunocomplexes. (cdc.gov)
  • We tested serum and CSF samples with standardized antibody assays for detection of Borrelia spp. (cdc.gov)
  • Develop and validate your molecular diagnostic assays faster with ready-to-use quantitative genomic and synthetic molecular standards and heat-inactivated preparations. (atcc.org)
  • No.: Z03484) in functional ELISA assay. (genscript.com)
  • End-point analyses, like ELISA, are less informative and rely upon labeling the reactants and adsorbing one of them, which can spoil their native activity, give high non-specific background signals, and limit the scope of an assay. (intechopen.com)
  • Support the development and validation of your assays with orthopoxviruses and nucleic acids from ATCC. (atcc.org)
  • Consecutive pathway and cluster analysis led to the identification of biological processes, namely processes involving binding and processing of nucleic acids, LRP1-dependent endocytosis, and exosome formation. (degruyter.com)
  • 2) By Method: Cellular Assay, Biochemical Assay, In Silico. (thebusinessresearchcompany.com)
  • Here we will establish biochemical, biophysical and cellular platforms to systematically dissect the biological importance of RNA G4s in cellular physiology. (grantome.com)
  • G4 structural and functional studies have largely focused on DNA G4s, and the number of biological functions assigned to these motifs has grown rapidly since the discovery of their involvement in telomere biology. (grantome.com)
  • In AIM 3, we will use functional assays to determine the biological significance of RG4s in mRNA stability, localization and translation. (grantome.com)
  • probes, molecular reporters and fluorescent indicators for structural or functional imaging or microscopy/nanoscopy. (nih.gov)
  • In recent years, it has been shown that P. falciparum ( Pf ) possesses a functional low molecular weight thiol thioredoxin (Trx) system [ 4 ]. (biomedcentral.com)
  • ab a Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 4301 West Markham Street (slot 516), Little Rock, AR 72205-7199, USA. (rsc.org)
  • Through its funding of the MLPCN, NIH will support the costs of assay automation, screening and optimization chemistry. (nih.gov)
  • Analytical techniques in clinical chemistry for the estimation of polypeptide hormone concentrations in biological fluids are changing from limited reagent, radioimmunoassay methods to excess reagent immunoradiometric methods, with a consequential improvement in sensitivity, precision and speed. (ucl.ac.uk)
  • I want to get the latest chemistry news from C&EN in my inbox every week. (acs.org)
  • Here, we present the structure of the core Sec61/TRAP complex bound to a mammalian ribosome by cryogenic electron microscopy (cryo-EM). (lu.se)
  • Building and utilizing genetic constructs is the core of molecular biology, and is key to important efforts to develop vaccines and other medical advances. (codondevices.com)
  • The term systems toxicology is derived from systems biology and could be defined as the study of biological systems, using omics technologies, with a focus on the mechanisms underlying complex biological processes, their interactions and perturbations in response to a toxin combined with mathematical data integration and modeling. (brill.com)
  • In Methods in Molecular Biology (pp. 33-41). (ncsu.edu)
  • In Methods in Molecular Biology (pp. 163-174). (ncsu.edu)
  • PLOS Biology provides an Open Access platform to showcase your best research and commentary across all areas of biological science. (plos.org)
  • Characterize aptamer target interactions with respect to their thermodynamics parameters including enthalpy (ΔH), free enthalpy of binding (ΔG), entropy (ΔS), and stoichiometry using ITC. (2bind.com)
  • Exclude unwanted side-interactions in these biological matrices. (2bind.com)
  • Biostatistics areas of interest include development of advanced statistical techniques and methodologies for design of biological experiments, collection and analysis of the data from those experiments and interpretation of, and inference from, the results. (nih.gov)
  • It holds significance in myriad domains of biological drug discovery, encompassing cellular cultivation, innovative assay methodologies, strategic animal model systematization, in-depth pharmacokinetic & pharmacodynamic (PK/PD) modeling, and intricate mechanism of action (MOA) investigations. (genemedi.net)
  • With the recent advances in human-cell cultivation techniques, allowing in vivo -like in vitro long-term functionality, there is a shift in focus towards the mechanistic details of the adverse effects "over time" aimed at a better understanding of the dynamics of biological processes. (brill.com)
  • This lactone ring is a Michael acceptor that is known to affect biological processes such as cell proliferation, death/apoptosis, and cell migration, by interfering with nucleophilic sites in cell signalling pathways. (lu.se)
  • In the last part, information about cell-penetrating peptides that can be used as molecular carries is mentioned with providing classification and cellular uptake mechanism of them. (intechopen.com)
  • This work will elucidate how RG4-mediated functions contribute to cellular mRNA homeostasis, and will identify physiologically significant RG4-binding partners, which in turn may reveal molecular targets and pathways with therapeutic potential. (grantome.com)
  • He was the very first person I recruited in a group of 40, and he was just amazing," says Kay Ahn, who was Janssen's global head of molecular and cellular pharmacology at the time. (acs.org)
  • The work will be of broad interest to molecular and cellular neuroscientists as well as biochemists. (elifesciences.org)
  • Patients 1 and 4 experienced their first febrile episode in Africa and were empirically treated with antimalarial drugs without biological confirmation of Plasmodium infection. (cdc.gov)
  • To address this, concentrations of testosterone, sex hormone binding globulin, androstanediol glucuronide (a metabolite of dihydrotestosterone) and estradiol were measured in stored serum specimens from men examined in the morning sample of the first phase of NHANES III (1988-1991). (cdc.gov)
  • In these circumstances, modestly sensitive estradiol assays suffice. (medscape.com)
  • However, numerous other clinical applications warrant the use of estradiol assays with extra sensitivity, simultaneous estrone testing, or both. (medscape.com)
  • This FOA promotes discovery and development of new chemical probes as research tools for use by scientists in both the public and private sector to advance the understanding of biological functions and disease mechanisms. (nih.gov)
  • These findings may have significant implications for the strength, specificity and kinetics of lipid binding. (lu.se)
  • These methods have been used to ascertain whether our monoclonal antibodies to hFSH are selective in binding predominantly to the biologically active isoforms of FSH. (ucl.ac.uk)
  • Some of the common methods available for determining cyanide in biological media are reported in Table 7-1. (cdc.gov)
  • or use data science methods and tools to deduce information about biological systems. (nih.gov)
  • Of interest are development of computational and mathematical algorithms and tools, modeling techniques and approaches for understanding the complexity of biological systems, and utilization of big datasets and data science methods for model construction. (nih.gov)
  • molecular methods and serodiagnostic tests for Lyme disease might be helpful in its detection. (cdc.gov)
  • Recently, molecular methods have been shown to be more reliable for Borrelia spp. (cdc.gov)
  • Molecular complexes of topo I and topo II inhibitors from camptothecin and flavonoid families with model DNA oligomers. (science24.com)
  • however, the simulations are unable to reproduce covalent inhibitors that bind irreversibly at the active site. (biomedcentral.com)
  • For SARS-CoV-2 molecular diagnostics manufacturers moving from EUA to 510(k) premarket submission to FDA, ATCC provides a variety of clinically relevant materials for evaluating limit of detection, inclusivity, and cross-reactivity. (atcc.org)
  • The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. (rndsystems.com)
  • Combining bioassay screening with the cheminformatics strategy of LC-MS/MS molecular networking as a discovery tool expedited the targeted isolation of a natural product possessing both a novel chemical structure and a desired biological activity. (henryford.com)
  • These isohormones exhibit differences in carbohydrate composition, receptor binding activity, plasma half life and in-vitro and in-vivo bioactivity. (ucl.ac.uk)
  • Here, we demonstrate the biological activity and stereoselectivity of the most active compound from this series. (rcsb.org)
  • A comparison with other disulfide reductases including glutathione reductases reveals the most common inhibitor binding sites are at the active site and at the crystallographic 2-fold axis in the large cavity at the dimer interface. (biomedcentral.com)
  • The proposed studies may elucidate molecular events underlying normal and pathological aspects of cell physiology, and identify events contributing to the tumorigenic or neurodegenerative changes. (grantome.com)
  • Our data confirm that VOC, particularly those such as B.1.351 with substitutions at residues 484 and 417, escape neutralization by antibodies directed to the ACE2-binding Class 1 and the adjacent Class 2 epitopes but are susceptible to neutralization by the generally less potent antibodies directed to Class 3 and 4 epitopes on the flanks of the RBD. (nature.com)
  • The molecularly imprinted polymers act as artificial antibodies, artificial receptors, or artificial enzymes with the added benefit over their biological counterparts of being highly durable. (mdpi.com)
  • The same combination of monoclonal antibodies has been used to develop a more sensitive time resolved immunofluorometric assay for hFSH. (ucl.ac.uk)
  • Our current knowledge of hFSH microheterogeneity and the assays available indicates the urgent need to select antibodies with specific binding to the biological active forms of the hormone. (ucl.ac.uk)
  • The biofunction of antibodies are validated with in-vitro assay (IC50 or EC50 TBD). (genemedi.net)
  • GMab, developed by GeneMedi, constitutes an advanced library of recombinant monoclonal antibodies, each meticulously designed to target specific molecular entities. (genemedi.net)
  • I-FABP) and bovine heart fatty acid-binding pro- tissues and in intracellular as well as extracellular tein (H-FABP). (lu.se)
  • In this review we summarize the current state of the art in detailing the three main categories of quadruplex-binding macrocycles described so far (telomestatin-like polyheteroarenes, porphyrins and derivatives, polyammonium cyclophanes), and in addressing both synthetic issues and biological aspects. (hindawi.com)
  • We highlight key features of the method that enhance assay accuracy and precision. (cdc.gov)
  • Both forms of IL-1 bind to the same receptors, designated type I and type II. (rndsystems.com)
  • VEGF is presented as an antiparallel homodimer, and its binding to VEGFR-2 brings two receptors in close proximity. (open.ac.uk)
  • This initiative is one of the integrated components of the NIH Molecular Libraries Roadmap initiative that offers biomedical researchers access to large-scale automated high throughput screening (HTS) centers in the MLPCN, diverse compound libraries in the Small Molecule Repository (MLSMR) and information on biological activities of small molecules in the PubChem BioAssay public database. (nih.gov)
  • 17-19 Among these, the simulations by molecular dynamics (MD) simulations. (lu.se)
  • Our aim was to further investigate allosteric inhibition using binders targeting extracellular subdomains 4-7 of VEGFR-2 as an alternative to existing anti-angiogenic therapies, which rely on neutralizing VEGF or blocking of the ligand-binding site on the receptor. (open.ac.uk)
  • Typically, they harness an optical phenomenon to detect the binding of a solution molecule (analyte) to its immobilized partner (ligand) on a sensor and record the output as a "sensorgram", which tracks the binding signal in arbitrary units proportional to mass sensed at the surface of the biosensor as a function of time. (intechopen.com)
  • Frankfurt am Main, Germany about half of the crystallographically visible water molecules appear to be expelled by the ligand, we find that ligand binding actually increases the number of water molecules within the cavity. (lu.se)
  • Aptamers are state-of-the-art macromolecular drugs with clinical applications, as well as synthetic binding and sensory tools for industrial diagnostics and basic research. (2bind.com)
  • drugs that bind their targets tightly can be administered at lower levels than those that bind weakly and thus are less likely to exhibit undesirable side-effects and are more cost-effective. (intechopen.com)
  • Benefit from our fast and robust aptamer assays for selecting a pair of aptamers against the same target for the development of aptamer-based sandwich diagnostic tools. (2bind.com)
  • Chapter Six focuses on the latest advances in the development of the telomerase assays and the authors further discuss the application potentiality of the assays for clinical samples. (novapublishers.com)
  • Immunoassay precision is defined as the reproducibility between wells within an assay (intra-assay) and between assays (inter-assay). (stratech.co.uk)
  • Since the most of the products possessed a very low solubility in the aqueous buffer medium used in biological assays, polar groups (nitro, succinamidyl and trimethyl-amino substituent in position 6 of the chromone ring or hydroxyl group on the benzene ring in the amide part have been inserted to the molecule. (uni-wuerzburg.de)
  • I-LBP, ileal lipid-binding to the aqueous solubility of the fatty acid. (lu.se)
  • An advanced toxicology laboratory is available for highly specialized toxicological work such as genomics, metabolomics and molecular biological studies (e.g. qPCR). (vu.nl)
  • This is because FSH is not an homogeneous molecular species. (ucl.ac.uk)
  • The heavy chain is responsible for both receptor some species of the genus Clostridium, in particular, Clostridium binding via its C-terminal (CT) binding domain [4,5] (HC) and botulinum, C. butyricum, C. baratii, and C. argentinense. (cdc.gov)
  • Chapter Two discusses the G-quadruplexes and i-motifs as scaffolds for molecular engineering of DNA aptamers. (novapublishers.com)
  • Our results show that small structural changes produce ligands with similar binding affinities ( K d ) for TNF , but significantly different potencies in a L929 cell-based assay . (rsc.org)
  • Optimization of the rebinding solvent indicated that ethanol-water at a molar ratio of 6:4 (v/v) was the best solvent system as it enhanced the rebinding performance of the imprinted polymers toward both tocopherol and tocopherol acetate with a binding capacity of approximately 2 mg/g of polymer. (mdpi.com)
  • The inherent complexity of biological systems is a challenge that is expected to be overcome by computational modeling of biological systems. (brill.com)
  • Integrating LC-MS/MS molecular networking and bioassay-guided fractionation enabled the targeted isolation of a new and bioactive cyclic octapeptide, samoamide A (1), from a sample of cf. (henryford.com)
  • Lipoproteins are complex molecular assemblies that are key participants in the intricate cascade of extracellular lipid metabolism with important consequences in the formation of atherosclerotic lesions and the development of cardiovascular disease. (cdc.gov)
  • The H5N1 Inventory supports a molecular-based approach for surveillance and should be used to identify genetic mutations that determine viral phenotypic characteristics of importance. (cdc.gov)
  • A fractionated version of heparin, known as low molecular weight heparin, is also available. (wikipedia.org)
  • The androgen testosterone (17 -hydroxyandrostenone) has a molecular weight of 288 daltons. (cdc.gov)
  • In order to address these challenges effectively, there is an urgent need to understand the biological consequences of the mutations found in these lineages, and the consequential impact on their susceptibility to current control measures, particularly vaccines. (nature.com)