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OligonucleotidesOligonucleotides, AntisenseBase SequenceOligodeoxyribonucleotidesOligoribonucleotidesMolecular Sequence DataPhosphorothioate OligonucleotidesOligodeoxyribonucleotides, AntisenseDNANucleic Acid ConformationOligonucleotide ProbesThionucleotidesNucleic Acid DenaturationOligoribonucleotides, AntisenseNucleic Acid HybridizationRNA, MessengerRibonuclease HOrganophosphorus CompoundsRNADNA, Single-StrandedPolymerase Chain ReactionNucleic AcidsCloning, MolecularAmino Acid SequenceDNA, AntisenseRibonuclease T1GuanineBinding SitesIndicators and ReagentsDNA-Binding ProteinsBase PairingIntercalating AgentsTranscription, GeneticDNA PrimersKineticsRibonucleasesBase CompositionCell LineTransfectionPsoralensDNA ProbesGenes, SyntheticPhosphoric AcidsEscherichia coliFluorescent DyesPeptide Nucleic AcidsPolydeoxyribonucleotidesPromoter Regions, GeneticBase Pair MismatchTumor Cells, CulturedNucleic Acid HeteroduplexesAptamers, NucleotideCells, CulturedMolecular StructureRNA, ViralTemperatureMorpholinosPolyribonucleotidesSubstrate SpecificityThermodynamicsPoly TPlasmidsGene TargetingThymineGene Expression RegulationHeLa CellsTranscription FactorsFicusinGene ExpressionZebrafishDrug StabilityG-QuadruplexesStructure-Activity RelationshipMutationCytosineCell DivisionOligonucleotide Array Sequence AnalysisGuanosineImmobilized Nucleic AcidsRNA, AntisenseChromatography, High Pressure LiquidPhosphodiesterase IProtein BindingCross-Linking ReagentsSequence Homology, Nucleic AcidPolynucleotidesNucleosidesCircular DichroismTemplates, GeneticDNA, ViralPurinesGlassMagnetic Resonance SpectroscopyExonucleasesSequence Analysis, DNADNA, ComplementaryElectrophoresis, Polyacrylamide GelDown-RegulationZebrafish ProteinsXanthopterin
Antisense oligonucleotidesSynthesis2022BiologySingle-strandedChimericPhosphorothioateBiosciencesHybridizationInternational JournalSyntheticTherapy2017MRNAAssayStrandsHairpinDouble-strandedSequence2023Nucleic acidCharacterizationSequencesASOsAntigensCellularTherapeuticsBeacon containsVitroFrontiersProteinGenesDiagnosticsDetectionClinicalCentreResearchAbstractBrainMethodologiesGeneticSimulationsDrug resistanceDynamicsBiotechnologyAnalysisBackboneDevelopmentsReportDiseases
Antisense oligonucleotides3
  • An efficient and scalable synthesis of new oligonucleotide monomers was developed for replacement of the phosphodiester backbone of RNA by a sulfonamide-containing backbone to enable construction of sulfonamide antisense oligonucleotides (SaASOs). (burleylabs.co.uk)
  • A promising therapy for a range of brain diseases involves antisense oligonucleotides (ASOs)-;specialized molecules that can modulate RNA and alter protein production-;directly injected into the cerebrospinal fluid, in the space around the brain and spinal cord. (news-medical.net)
  • Antisense oligonucleotides (ASOs), defined as short synthetic oligonucleotides with single-stranded sequences complementary to certain mRNA sites, have been under drug development for approximately 30 years, with the first FDA-approved drug, Fomivirsen, being approved in 1998 ( Stein and Castanotto, 2017 ). (aspetjournals.org)
Synthesis7
  • Automated synthesizers allow the synthesis of oligonucleotides up to 160 to 200 bases. (news-medical.net)
  • Oligonucleotide Synthesis Market Report 2022: The RNA Bas. (pharmiweb.com)
  • DUBLIN--(BUSINESS WIRE)--The "Oligonucleotide Synthesis Markets - Forecasts by Oligo Length, Application, and Product - With Executive and Consultant Guides and including Customized Forecasting and Analysis" report has been added to ResearchAndMarkets.com's offering. (pharmiweb.com)
  • 2.1 What is Oligonucleotide Synthesis? (pharmiweb.com)
  • The oligonucleotide synthesis market is expected to register a CAGR of 12.3% over the forecast period. (mordorintelligence.com)
  • COVID-19 has impacted the growth of the oligonucleotide synthesis market. (mordorintelligence.com)
  • Moreover, various companies are investing in oligonucleotide synthesis, which is expected to increase market growth over the forecast period. (mordorintelligence.com)
20222
  • In a study published in PLOS One in March 2022, it was observed that the oligonucleotides successfully targetted 5'-polyUs in the coronavirus genome. (mordorintelligence.com)
  • Frontiers in molecular biosciences 2022 0 0. (cdc.gov)
Biology6
  • and proteins from the same stretch of DNA, plays an important role in molecular biology. (news-medical.net)
  • 1 Department of Dermatology and Cutaneous Biology, Department of Biochemistry and Molecular Pharmacology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University and Jefferson Medical College, Philadelphia, PA 19107, USA. (nih.gov)
  • pp. 395-417 (Methods in Molecular Biology). (johnshopkins.edu)
  • Advances in molecular biology techniques illuminate the genetic basis underlying all types of MD: defects in the genetic code for dystrophin, a 427-kd skeletal muscle protein (Dp427). (medscape.com)
  • The molecular interactions of genes and gene products underlie fundamental questions of biology. (lu.se)
  • The advent of the nucleic acid labeling technique, coupled with the development of genomics, has significantly increased the use of molecular biology enzymes, kits, and reagents for the detection of pathogenic events at the genomic level. (technavio.com)
Single-stranded2
  • Surprisingly, these short single-stranded oligonucleotides (ODN) showed a similar gene correction frequency to chimeric RNA-DNA oligonucleotide, measured using the same system. (nih.gov)
  • The beacon contains a single-stranded oligonucleotide linker that forms a stem-loop structure (hairpin) in which the sequence is an antisense oligonucleotide (AS-ON) complementary to a target mRNA. (utoronto.ca)
Chimeric3
  • Antibody-oligonucleotide conjugates or AOCs belong to a class of chimeric molecules combining in their structure two important families of biomolecules: monoclonal antibodies and oligonucleotides. (wikipedia.org)
  • These results show that a relatively short ODN can make a sequence-specific change in the target sequence in mammalian cells, at a similar frequency as the chimeric RNA-DNA oligonucleotide. (nih.gov)
  • Unmodified RNA, RNA with modifications, chimeric oligonucleotides with mixed DNA and RNA bases, 2'-OMe-RNA, 2'-F-RNA, and other antisense RNAs are available at your specific request. (genscript.com)
Phosphorothioate2
  • This oligonucleotide base pairs with the actual antisense oligonucleotide (gapmer bearing phosphorothioate linkages and 2'-deoxy-2'-fluoro-beta-D-arabinonucleic acid modifications and a terminal fluorophor) aiming for an increased RNaseH activity. (wikipedia.org)
  • Given that phosphorothioate modifica-tion is the most common method of enhancing oligonucleotide stability in vivo, we expect MRM-based assays constructed by a similar methodology to be applicable across a variety of therapeutic oligonucleotides. (shimadzu.com)
Biosciences1
  • The Molecular Inversion Probe assay (Parallele Biosciences, South San Francisco, Calif, USA) [ 6 ] uses probes that can be circularized and contain two sequences complementary to regions adjacent to SNPs in the DNA target in the first step of the genotyping reaction. (hindawi.com)
Hybridization2
  • The current study describes the implementation of a hybridization liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform for the direct quantitation of antisense oligonucleotide (ASO) ƒ u . (aspetjournals.org)
  • Molecular architectures for electrocatalytic amplification of oligonucleotide hybridization. (mpg.de)
International Journal1
  • International Journal of Molecular Sciences , 23(2), article no. 760. (open.ac.uk)
Synthetic7
  • Molecular shuttles are the basis of some of the most advanced synthetic molecular machines. (nature.com)
  • Our findings could open avenues for the real-time characterization of synthetic devices at the single molecule level, and provide crucial information for designing molecular machinery able to operate under physiological conditions. (nature.com)
  • Here, we exploit the properties of optical tweezers to quantify the real-time operation of individual synthetic molecular shuttles. (nature.com)
  • Synthetic Oligonucleotides are poised for explosive growth as gene technology moves into mainstream healthcare, food production and, even, Data Storage! (pharmiweb.com)
  • The market for small snippets of DNA, called Oligonucleotides, is growing into a market for Synthetic DNA where organisms, like algae, are "custom designed" to produce food and medicine while consuming few resources. (pharmiweb.com)
  • The Medicines Manufacturing Innovation Centre launched a collaboration with AstraZeneca, Exactmer, Novartis, and UK Research and Innovation to develop a scalable, sustainable, and more cost-effective medicines manufacturing process for oligonucleotides: short strands of synthetic DNA or RNA. (mordorintelligence.com)
  • Therapeutic oligonucleotides are synthetic oligonucleotides composed of 10-30 nucleic acid bases. (shimadzu.com)
Therapy3
  • Patient-Customized Oligonucleotide Therapy for a Rare Genetic Disease. (childrenshospital.org)
  • Molecular Therapy - Methods and Clinical Development. (lu.se)
  • Molecular therapy : the journal of the American Society of Gene Therapy 2023 0 0. (cdc.gov)
20171
MRNA2
  • We report a new class of photodynamic molecular beacon (PMB) with tumor specific mRNA-triggered control of singlet oxygen (1O2) production. (utoronto.ca)
  • mRNA was extracted using TRizol, Affymetrix oligonucleotide 230A genechip analysis performed and analyzed statistically (3-4/group). (cdc.gov)
Assay2
  • The GoldenGate assay (Illumina, San Diego, Calif, USA) [ 7 , 8 ] is based on a similar principle but includes two oligonucleotides flanking the SNPs for the genotyping reactions. (hindawi.com)
  • This article describes using a liquid chromatograph/mass spectrometer to determine molecular mass and construct a quantitative assay for a therapeutic oligonucleotide and its analogs. (shimadzu.com)
Strands1
  • Anti-fouling characteristics of surface-confined oligonucleotide strands bioconjugated on streptavidin platforms in the presence of nanomaterials. (mpg.de)
Hairpin2
  • Molecular beacons are hairpin shaped fluorescent oligonucleotide probes that can report the presence of a completely complimentary DNA target. (usda.gov)
  • When the molecular beacon is not annealed to a complementary target the hairpin structure places the fluorophore and the quencher in close proximity preventing fluorescence. (usda.gov)
Double-stranded2
  • We show that coupling of a molecular shuttle with double-stranded DNA (dsDNA) enables its single-molecule manipulation under aqueous conditions. (nature.com)
  • To overcome the issue, specifically designed PCR platform, STexS ( S NP t yping with ex cellent s pecificity) using double stranded oligonucleotides was implemented as a means to emphasize the amplification of mutated SNP templates by decreasing unwanted amplification of 3'mismatched DNA copies. (researchsquare.com)
Sequence3
  • Click molecule labels to explore molecular sequence information. (nih.gov)
  • Deoxyoligonucleotides showed a higher frequency of gene correction than ribo-oligonucleotides of the identical sequence. (nih.gov)
  • Using synchronized cells undergoing recombination that is initiated at a specific site on a chromosome by an inducible endonuclease, we use physical monitoring techniques (Southern blots, PCR analysis) to follow the sequence of molecular events that occur in real time. (brandeis.edu)
20231
  • A new research paper was published in Oncotarget's Volume 14 on May 26, 2023, entitled, "Deconstructing the role of MALAT1 in MAPK-signaling in melanoma: insights from antisense oligonucleotide treatment. (news-medical.net)
Nucleic acid1
  • Nucleic acid labeling is widely used by end-users for the detection of molecular infectious diseases. (technavio.com)
Characterization1
  • Molecular characterization and gene expression of lhcb5 gene encoding CP26 in the light-harvesting complex II of Chlamydomonas reinhardtii. (bio.net)
Sequences1
ASOs1
  • Owing to their physical properties, such as relatively high molecular weight, linear structure, and nonspecific binding, ASOs present unique challenges in ƒ u determination through traditional techniques. (aspetjournals.org)
Antigens2
  • Cellular Components of the Immune System The immune system consists of cellular components and molecular components that work together to destroy antigens. (msdmanuals.com)
  • and molecular components that work together to destroy antigens (Ags). (msdmanuals.com)
Cellular1
Therapeutics1
  • Combination of exceptional targeting capabilities of monoclonal antibodies with numerous functional modalities of oligonucleotides has been fruitful for a variety of applications with AOC including imaging, detection and targeted therapeutics. (wikipedia.org)
Beacon contains1
  • The 5' end of a molecular beacon contains a fluorescent dye and the 3' end a fluorescence-quenching moiety. (usda.gov)
Vitro1
  • The in vitro gene correction induced by ODN in nuclear extracts was not dependent on the length or polarity of the oligonucleotide. (nih.gov)
Frontiers1
  • Frontiers in Molecular Neuroscience. (lu.se)
Protein3
  • Protein Expression and Purification, molecular modelling, and molecular dynamics simulation. (umassmed.edu)
  • Protein Expression and Purification, Computational Modeling, Molecular Dynamic Simulations, X-ray Crystallography, Enzyme Activity and Enzyme Inhibition Assays, Structure Aided Drug Design. (umassmed.edu)
  • The molecular defect occurs in the mitochondrial trifunctional protein (MTP). (medscape.com)
Genes1
  • Resistance to TB drugs in M. tuberculosis is caused by mutations in the corresponding drug resistance genes (e.g., katG, inhA, rpoB, pncA, embB, rrs, gyrA, gyrB), and detection of these mutations can be a molecular indicator of drug resistance. (johnshopkins.edu)
Diagnostics4
  • The increased demand for COVID-19 diagnostics has, in turn, increased the global demand for oligonucleotide raw materials that form the basis of molecular diagnostic products. (mordorintelligence.com)
  • For instance, in September 2021, SpeeDx invested in local oligonucleotide manufacturing to reduce supply chain risk and develop a more vital sovereign capacity for critical diagnostics. (mordorintelligence.com)
  • Other significant factors include the increasing government investments and R&D expenditure in pharmaceutical and biotechnology companies and the use of synthesized oligonucleotides in molecular diagnostics and clinical applications. (mordorintelligence.com)
  • Furthermore, the increasing adoption of oligonucleotides in molecular diagnostics and clinical applications contributes to market growth. (mordorintelligence.com)
Detection1
  • The method provides substantial improvements, including minimal matrix effects and high specificity when compared with previously used oligonucleotide ƒ u detection methods such as ligand binding assays or liquid scintillation. (aspetjournals.org)
Clinical1
  • Clinical, immunological and molecular characteristics of 37 Iranian patients with X-linked agammaglobulinemia. (lu.se)
Centre1
Research7
  • Many of these molecular devices are currently the subject of intense research interest due to their many potential applications in diverse fields from biomedicine to nanorobotics and their kinetics and thermodynamics in bulk are well understood 3 . (nature.com)
  • Intellegens, a company applying machine learning (ML) to accelerate innovation in R&D, today announced that it has secured a £1.6m grant from Innovate UK, part of UK Research and Innovation (UKRI), to apply ML in the emerging field of oligonucleotide therapies. (news-medical.net)
  • 1 Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037. (nih.gov)
  • Articles published in Journal of Molecular Pharmaceutics & Organic Process Research have been cited by esteemed scholars and scientists all around the world. (omicsonline.org)
  • Journal of Molecular Pharmaceutics & Organic Process Research has got h-index 14, which means every article in Journal of Molecular Pharmaceutics & Organic Process Research has got 14 average citations. (omicsonline.org)
  • Following are the list of articles that have cited the articles published in Journal of Molecular Pharmaceutics & Organic Process Research . (omicsonline.org)
  • Such research studies have impacted the demand and adoption for oligonucleotides in drug development, affecting the market growth during a pandemic. (mordorintelligence.com)
Abstract1
  • Abstract The 90 kD heat shock proteins (Hsp90) are molecular chaperones that are responsible for the folding of select proteins, many of which are directly associated with cancer progression. (sbir.gov)
Brain2
Methodologies2
  • Utilizing computational methodologies, including molecular modeling and conventional molecular dynamics simulations to understand the relationship between the three-dimensional structures, dynamics and the biological functions of DNA-binding enzymes and proteins central to Type I Diabetes. (umassmed.edu)
  • A fusion of cutting-edge methodologies, intertwining molecular dynamics simulations and the power of machine learning. (umassmed.edu)
Genetic3
  • Electrophysiology-Guided Genetic Characterisation Maximises Molecular Diagnosis in an Irish Paediatric Inherited Retinal Degeneration Population. (cdc.gov)
  • Molecular genetic epidemiology : a laboratory perspective / Ian N.M. Day, ed. (who.int)
  • Over the past few decades, the diverse molecular genetic causes of SCID have been identified with progress from studies of the immune system. (medscape.com)
Simulations1
  • molecular simulations. (lu.se)
Drug resistance2
  • In this chapter, we describe the utility of a microarray-based approach exploiting short overlapping oligonucleotides (sliding-frame array) to rapidly detect drug resistance-associated mutations (substitutions, deletions, and insertions) in the pncA gene responsible for resistance ofM. (johnshopkins.edu)
  • To understand and combat the HIV-1 Protease drug resistance at the molecular level to design potent inhibitors. (umassmed.edu)
Dynamics1
  • Here, we use optical tweezers to measure the mechanics and dynamics of individual molecular shuttles in aqueous conditions. (nature.com)
Biotechnology1
  • Additionally, the rising company and government initiatives to boost the development of oligonucleotide-based drug products and the adoption of oligonucleotide technology in the pharmaceutical and biotechnology industry are also expected to increase market growth. (mordorintelligence.com)
Analysis3
  • This investigation provides a detailed histological and molecular analysis of thrombus organization in the swine aneurysm model. (thejns.org)
  • The compounds used in the analysis were the therapeu-tic oligonucleotide mipomersen and three mipomersen analogs. (shimadzu.com)
  • Studies on HCV genotypes in people of HCV-specific antibodies and the latter with haemophilia have identified types 1, was used for molecular analysis. (who.int)
Backbone1
  • A straightforward uptake, like for most small-molecule drugs, is hindered by the polyanionic backbone and the molecular size of ONs. (wikipedia.org)
Developments1
  • Such developments are expected to increase the adoption of oligonucleotides in drug development which is anticipated to fuel the market growth over the forecast period. (mordorintelligence.com)
Report2
  • The Report Covers Global Oligonucleotide Market Outlook & Size. (mordorintelligence.com)
  • Herein, we report on a specific oligonucleotide-based RNA inhibitor targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). (mdpi.com)
Diseases1
  • It is the prototype of the primary immunodeficiency diseases and is caused by numerous molecular defects that lead to severe compromise in the number and function of T cells, B cells, and occasionally natural killer (NK) cells. (medscape.com)