• We found that Plk1 suppressed the activity of RSK1 during embryonic mitosis and that Plk1 was inhibited during embryonic diapause and mitotic arrest. (biospraysehatalami.com)
  • Therefore it would be interesting to examine the control mechanism of cell cycle progression in which RSK1 affects Myt1 in meiosis and Plk1 affects Myt1 in mitosis. (biospraysehatalami.com)
  • EB1-null cells also exhibit decreased adhesion during mitosis. (biologists.com)
  • All DMSO-treated mitotic cells (100%, n=18) advanced normally through cell division (Figs. 4A, B). In comparison, the huge most of cells which were uncovered to eupatorin at M phase left mitosis prematurely with defective cytokinesis. (mirnamimics.com)
  • Remarkably, within the same assay we observed that cells which were uncovered to eupatorin before they joined mitosis showed a mitotic delay upon admission to M phase rather than going through a premature mitotic xl880 exit. (mirnamimics.com)
  • All of the cells that enteredM phase in the existence of the flavonoid (n=41 cells supervised) were postponed at mitosis not less than 270 min before abnormal exit, the typical period of mitosis being 489±156 min (Fig. 4C). (mirnamimics.com)
  • The typical period of mitosis in DMSO-treated control cells was 64±28 min (n=23, Fig. 4C). (mirnamimics.com)
  • Eupatorin affects spindle formation, spindle integrity and centrosome separation To know why cells uncovered to eupatorin at G2 were postponed in mitosis we looked into when the flavonoid intervenes using the spindle dynamics, structure and/or MT polymerization. (mirnamimics.com)
  • Cells were blocked in mitosis with monastrol which induces monopolar spindles because of Eg5 inhibition.After that cells were launched either into normal or eupatorin-that contains culture medium both compounded with MG132 to avoid anaphase onset. (mirnamimics.com)
  • The term mitosis refers specifically to the process whereby the nucleus of the parent cell splits into two identical nuclei prior to cell division. (visionlearning.com)
  • This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. (lookformedical.com)
  • Downregulation of PI3K-C2β delays mitosis progression in PC3 and HeLa cells, resulting in reduced ability to form colonies in clonogenic assays in vitro. (biomedcentral.com)
  • In addition, these drugs are known to induce serious side effects due to inhibition of mitosis in other proliferating cells (mainly bone marrow and gut) as well as inhibition of other microtubules-dependent functions, such as neuronal processes [ 18 , 19 ]. (biomedcentral.com)
  • Upon treatment with Plk1 inhibitors, p53 in tumor cells is activated and induces strong apoptosis, whereas tumor cells with inactive p53 arrest in mitosis with DNA damage. (oncotarget.com)
  • Plk1, the most thoroughly characterized member among the mammalian Plks, has multiple important roles in mitosis and cytokinesis, such as centrosome maturation, bipolar spindle formation, kinetochore-microtubule dynamics, activation of the anaphase promoting complex, chromosome segregation and execution of cytokinesis [ 3 , 4 , 13 ]. (oncotarget.com)
  • Jobs for NDE1 and NDEL1 in mitosis have already been borne out by evaluation of non-neuronal cells and (refs 4 5 This observation shows that NDE1 may be involved in several facet of neural progenitor proliferation. (biotech-angels.com)
  • Mitosis could be symmetric leading to self-renewal from the neural progenitor pool or asymmetric resulting in one neural progenitor and the post-mitotic neuron or an intermediate progenitor each which migrate from the ventricle. (biotech-angels.com)
  • It is predicted that MCPH gene mutations may lead to the disease phenotype due to a disturbed mitotic spindle orientation, premature chromosomal condensation, signalling response as a result of damaged DNA, microtubule dynamics, transcriptional control or a few other hidden centrosomal mechanisms that can regulate the number of neurons produced by neuronal precursor cells. (biomedcentral.com)
  • Weighed against its homologs Plk1 (and its own connected signaling pathway) offers attracted much interest because overexpression of Plk1 can be firmly correlated with carcinogenesis (10 11 Furthermore inhibition of Plk1 using RNA disturbance (RNAi) or particular little molecule inhibitors causes development arrest or apoptosis in tumor cells (12-14). (biospraysehatalami.com)
  • Activation of AMPK by low energy supply and phosphorylation of LKB1 results in an inhibition of mTOR , thus decreasing energy-consuming processes, in particular translation and, thus, cell growth. (sdbonline.org)
  • GSK3 is certainly a constitutively energetic enzyme in regular cells and goes through fast inhibition by stimuli [2], [4]. (globaltechbiz.com)
  • RNAi of FACE1 protease results in growth inhibition of human cells expressing lamin A: implications for Hutchinson-Gilford progeria syndrome. (mpg.de)
  • Thus, inactive p53 is not associated with a susceptible cytotoxicity of Polo-like kinase 1 inhibition and could rather foster the induction of polyploidy/aneuploidy in surviving cells. (oncotarget.com)
  • In addition, compared to the mono-treatment, combination of Polo-like kinase 1 inhibition with anti-mitotic or DNA damaging agents boosts more severe mitotic defects, effectually triggers apoptosis and strongly inhibits proliferation of cancer cells with functional p53. (oncotarget.com)
  • Treatment of vector control K562 cells with the autophagy inhibitors 3-methyladenine and bafilomycin A1 recapitulated the growth kinetics, vacuolar morphology and LC3-II accumulation of CSN2 knockdown cells indicating that the cellular phenotype of CSN2 cells arises from autophagy inhibition. (biomedcentral.com)
  • Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. (lookformedical.com)
  • STI571 therapy of serum starved cells for 48h did not induce apoptosis. (aktinhibitor.com)
  • Programmed ageing should not be confused with programmed cell death (apoptosis). (stevechase.org)
  • MCPH is a neurogenic mitotic disorder, though affected patients demonstrate normal neuronal migration, neuronal apoptosis and neural function. (biomedcentral.com)
  • Sirtuins are NAD + -dependent histone deacetylases regulating important metabolic pathways in prokaryotes and eukaryotes and are involved in many biological processes such as cell survival, senescence, proliferation, apoptosis, DNA repair, cell metabolism, and caloric restriction. (biomedcentral.com)
  • This HeLa cell is expressing two fluorescent proteins, including EGFP-CENP-B, a fluorescent protein that marks kinetochores (seen as paired dots oriented parallel to the x-axis and attached to opposit. (cellimagelibrary.org)
  • Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)
  • New monoclonal antibodies recognizing phosphorylated proteins in mitotic cells. (mpg.de)
  • The regulated expression and degradation of proteins are critical to all aspects of cell development and proliferation. (biomedcentral.com)
  • Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)
  • The mammalian sirtuin (SIRT) family, evolutionally conserved proteins belonging to class III histone deacetylases (HDACs), comprises seven members. (biomedcentral.com)
  • A substantial portion of the regulatory interactions in the higher eukaryotic cell are mediated by simple sequence motifs in the regulatory segments of genes and (pre-)mRNAs, and in the intrinsically disordered regions of proteins. (biomedcentral.com)
  • Four human cell lines and primary post-mitotic mouse cortical neurons support prime editing with varying efficiencies. (nature.com)
  • Loss of SCAR in both Dictyostelium and Drosophila leads to a similar mitotic phenotype, with severe mitotic blebbing, indicating conserved functionality. (biologists.com)
  • The addition of the DAD to mammalian cells induces actin filament formation, stabilizes microtubules, and activates SRF mediated transcription. (wikipedia.org)
  • However, in spite of their multipolar appearance, nearly all eupatorin-treated cells had two pericentrin positive centrosomes (83%, 27 cells from 32, Fig. 5A) suggesting that eupatorin induces acentrosomal pole formation. (mirnamimics.com)
  • AZD6244 In relaxation from the cells, cleavage furrow formation was started but cytokinesis was soon aborted leading to fusion of two daughter cells into one polyploid cell (Figs. 4A, B). These results indicate that eupatorin impairs normal SAC signaling and suppresses normal cytokinesis when put on otherwise unperturbed mitotic cells, which fits using the phenotype caused by Aurora B kinase inhibitors. (mirnamimics.com)
  • This phenotype resembles the problem noticed in AZD2281 cells pretreated rich in power of nocodazole/vinblastine as individuals cells were resistant against eupatorin-caused forced mitotic exit even if Aurora B grew to become restricted. (mirnamimics.com)
  • Not surprisingly, nearly all cells given monastrol for 4 h showed monopolar phenotype (93%, 28 cells from 30, Fig. 5B). (mirnamimics.com)
  • We conclude that CSN2 is required for CSN integrity and the stability of individual CSN subunits, and postulate that CSN2 loss results in a phenotype distinct from that of cells lacking CSN5 possibly as a consequence of altered CSN5 activity within a resultant CSN subcomplex. (biomedcentral.com)
  • Collectively, we anticipate that this method will allow high-resolution microscopic analysis of cellular processes involving cell cycle progression, such as checkpoint activation, DNA replication, and cellular division. (biomedcentral.com)
  • To review whether eupatorin intervenes with mitotic progression in unperturbed cells we shot cycling HeLa H2B-GFP cell populations soon after addition Afatinib of DMSO or eupatorin. (mirnamimics.com)
  • Alteration of signalling pathways regulating cell cycle progression is a common feature of cancer cells. (biomedcentral.com)
  • Mitotic spindles are three-dimensional molecular machines. (tu-dresden.de)
  • This approach allows us to reconstruct mitotic spindles in 3D with the resolution of about 2.3 nm. (tu-dresden.de)
  • 2017). My intention is to apply this approach now for a quantitative analysis of mitotic spindle structure in mammalian cells. (tu-dresden.de)
  • Nearly all cells which were uncovered to eupatorin at late G2 showed multipolar spindle structure with several small satellite rods (73%, 22 cells from 30, Fig. 5A) at M phase. (mirnamimics.com)
  • cell cycle arrest. (biospraysehatalami.com)
  • Here we show that encysted embryos (cysts) of the primitive crustacean are ideal for such research because they undergo WNT7A complete cell cycle arrest when they enter diapause (a state of obligate dormancy). (biospraysehatalami.com)
  • however commonly used animal models are limited in that cell cycle arrest must be induced by treatment with drugs. (biospraysehatalami.com)
  • This really is, however, an underestimate from the extent from the delay as numerous cells (n=23) remained as at mitotic arrest in the finish from the filming session. (mirnamimics.com)
  • CSN5 knockdown caused mitotic defects, G2/M arrest and apoptotic cell death. (biomedcentral.com)
  • Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)
  • Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
  • Metaphase Kinetochore Dynamics: In this HeLa cell, which is expressing both a kinetochore marker (EGFP-CENP-B) and a centrosome marker (Venus-centrin), one can visualize the dynamic nature of kinetoch. (cellimagelibrary.org)
  • These results reveal that the severity of (refs 3 4 5 Several of these genes are associated with centrosome and/or mitotic function suggesting that errors in neural progenitor cell proliferation contribute to disease pathology. (biotech-angels.com)
  • GSK3 inhibitors decrease the proliferation of Kinase Assay MCF7 cells had been lysed with Cell Lysis Buffer (Cell Signaling Technology, 9803). (globaltechbiz.com)
  • Plk1 inhibitors target all rapidly dividing cells irrespective of tumor cells or non-transformed normal but proliferating cells. (oncotarget.com)
  • Specific RNAi Mediated Gene Knockdown in Zebrafish Cell Lines. (mpg.de)
  • Mammalian RNAi constructs have been constructed as described. (aktinhibitor.com)
  • Examples of cell-based assays include RNAi [ 3 ], second messenger, cell proliferation, and reporter assays [ 4 ]. (oncotarget.com)
  • Fig. 5: Prime editing of pathogenic mutations, prime editing in primary mouse cortical neurons, and comparison of prime editing and HDR in four human cell lines. (nature.com)
  • Neurons are highly polarized cells with distinct axons and dendrites, and they have a high demand for energy to maintain neural circuits. (en-journal.org)
  • These provide as stem cells in charge of the production of most excitatory cortical neurons most glial cells and adult stem cells20 23 The RGP cells are extremely elongated with their apical and PRT062607 HCL basal processes spanning the entire thickness of the developing neocortex. (biotech-angels.com)
  • In an effort to further understanding of the effects of radiofrequency (RF) radiation on somatic cell mitotic activity and germ cell function, cell suspensions were exposed to 27 megahertz (MHz) or 2,450MHz radiation under isothermal conditions in-vitro. (cdc.gov)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • They are assembled to segregate chromosomes to the forming daughter cells (Movie 1). (tu-dresden.de)
  • To overcome the limitations brought upon by cellular fixation, membrane-permeant DNA fluorophores were designed to stain DNA stoichiometrically in live-cells (supravital staining), enabling both the quantification of DNA content and the ability to track the movement of chromosomes over time. (biomedcentral.com)
  • Similarly, the large ribosomal RNA gene clusters that reside on different chromosomes manage to find each other in almost every cell nucleus to form another easily discernable nuclear entity, the nucleolus [ 27 - 29 ]. (biomedcentral.com)
  • This interaction is important in promoting the capture and stabilization of a subset of microtubules oriented towards the leading edge of migrating cells. (wikipedia.org)
  • 286:517) for displaying the molecular polarity of microtubules (MTs) has been slightly modified and applied to the midbodies of cultured mammalian cells and the phragmoplasts of Haemanthus endosperm. (rupress.org)
  • Anti-mitotic drugs received considerable attention, especially following the early identification of the anti-cancer properties of microtubules-targeting agents, such as vinca alkaloids [ 15 ] and taxanes [ 16 ]. (biomedcentral.com)
  • Formins regulate the actin and microtubule cytoskeleton and are involved in various cellular functions such as cell polarity, cytokinesis, cell migration and SRF transcriptional activity. (wikipedia.org)
  • The class II PI3K isoform PI3K-C2β was downregulated in prostate cancer PC3 cells and cervical cancer HeLa cells using selective siRNAs and the effect on cell growth was determined in the absence or presence of the microtubule-stabilizing agent/anti-cancer drug docetaxel. (biomedcentral.com)
  • RNA interference by osmotic lysis of pinosomes: liposome independent transfection of siRNAs into mammalian cells. (mpg.de)
  • of c Abl signaling to MST kinases more builds the situation that c Abl can be a crucial regulator in neuronal cell death. (aktinhibitor.com)
  • After EGF stimulation for 4 h, Helix Pomatia Lectin (HPL) (green) in HeLa cells expressing constitutively active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) resists redistribution from Golgi (Giantin) (re. (cellimagelibrary.org)
  • Fig. 1: Overview of prime editing and feasibility studies in vitro and in yeast cells. (nature.com)
  • Indeed, Hbp1 accumulates in cells lacking GID/CTLH activity, and Hbp1 physically interacts and is ubiquitinated in vitro by reconstituted GID/CTLH complexes. (elifesciences.org)
  • Cells propagated in vitro in special media conducive to their growth. (lookformedical.com)
  • Compared to control cells, PC3 cells lacking PI3K-C2β form smaller and more compact colonies in vitro and they form tumours more slowly in vivo in the first weeks after cells implant. (biomedcentral.com)
  • The experiments were repeated for three times and quantative density is indicated Lysates of HEK 293T cells transfected with the FLAG MST2 or Y81F expression plasmid were immunoprecipitated with all the anti FLAG antibody and subjected to an in vitro kinase assay employing Histone H2B as substrate in the presence of ATP. (aktinhibitor.com)
  • Because NS is often observed during the mammalian CA test (in vitro), we examined the relationship between NS and CAs. (biomedcentral.com)
  • The cascade continued until several weeks later, millions of cells were dividing - powering the exponential pattern of growth that eventually formed all of the organs and tissues of your body. (visionlearning.com)
  • The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. (lookformedical.com)
  • It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION . (lookformedical.com)
  • Tumour growth in vivo was assessed using a xenograft model of PC3 cells upon PI3K-C2β downregulation and in combination with docetaxel. (biomedcentral.com)
  • Stable and transient PI3K-C2β downregulation potentiates the effect of low concentrations of docetaxel on cancer cell growth. (biomedcentral.com)
  • Furthermore, data indicate that blockade of PI3K-C2β might represent a novel strategy to potentiate the effect of docetaxel on cancer cell growth. (biomedcentral.com)
  • Loss of either CSN subunit dramatically reduced cell growth but resulted in distinct patterns of cell death. (biomedcentral.com)
  • Molecular Biology of the Cell. (wikipedia.org)
  • Trends in Cell Biology. (wikipedia.org)
  • However, the measurement of DNA content by flow cytometry provides little resolution to the biology of individual cells. (biomedcentral.com)
  • Five members of the Plk family have been discovered in humans and these serine/threonine kinases have emerged as key players by performing crucial functions in the cell cycle, DNA damage response and neuron biology [ 2 - 6 ]. (oncotarget.com)
  • The best understood of these involves myosin-II-dependent constriction around the cell equator, but both Dictyostelium and mammalian cells also use a parallel, adhesion-dependent mechanism to generate furrows. (biologists.com)
  • The latter mechanism facilitates a considerable degree of sister chromatid separation in securin-knockout cells that lack APC/C activity. (nature.com)
  • Thus, our results identify an unexpected function of SGO2 in mitotically dividing cells and a mechanism of separase regulation that is independent of securin but still supervised by the SAC. (nature.com)
  • Presented in conjunction with cells expressing a histone 2B-GFP fusion protein (H2B-GFP), we demonstrated how this method enabled chromosomal segregation errors to be tracked in cells as they progressed through cellular division that were later identified as either diploid or polyploid. (biomedcentral.com)
  • It also directly binds to BubR1, a kinetochore-associated kinase implicated in the mitotic checkpoint, the major cell cycle control pathway in which unattached kinetochores prevent anaphase onset. (rupress.org)
  • Thus, CENP-E is required for enhancing recruitment of its binding partner BubR1 to each unattached kinetochore and for stimulating BubR1 kinase activity, implicating it as an essential amplifier of a basal mitotic checkpoint signal. (rupress.org)
  • You can find six phosphorylation sites in RSK1 that are crucial for its activation and its own subsequent part in substrate phosphorylation (21 22 Of the phosphorylation of Ser-380 can be very important to RSK1 activation traveling functions such as for example rules of gene manifestation and protein synthesis and cell cycle regulation as a downstream Nisoxetine hydrochloride kinase in the Mos-MAPK pathway (23). (biospraysehatalami.com)
  • The master kinase LKB1 is a key regulator of several cellular processes, including cell proliferation, cell polarity and cellular metabolism. (sdbonline.org)
  • p53 represses the promoter of Polo-like kinase 1, whereas Polo-like kinase 1 inhibits p53 and its family members p63 and p73 in cancer cells lacking functional p53. (oncotarget.com)
  • In this regard, restoration of p53 in tumor cells with loss or mutation of p53 will reinforce the cytotoxicity of combined Polo-like kinase 1 therapy and provide a proficient strategy for combating relapse and metastasis of cancer. (oncotarget.com)
  • L5178Y is often used in mouse lymphoma L5178Y cell Tk (thymidine kinase) gene mutation assays. (biomedcentral.com)
  • Centromere-associated protein-E (CENP-E) is an essential mitotic kinesin that is required for efficient, stable microtubule capture at kinetochores. (rupress.org)
  • Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). (hmdb.ca)
  • Effects of expressing lamin A mutant protein causing Emery-Dreifuss muscular dystrophy and familial partial lipodystrophy in HeLa cells. (mpg.de)
  • Prognostic significance of tumor cell proliferation rate as determined by the MIB-1 antibody in breast carcinoma: Its relationship with vimentin and p53 protein. (mpg.de)
  • The precise regulations of pre-RC protein levels and assembly are effective ways to prevent reassembly of de novo MCM2-7 onto the replicated origins to re-license and re-replicate the genomic DNA in the subsequent phases of the same cell cycle ( Figure 1) . (intechopen.com)
  • Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. (thermofisher.com)
  • Live-cell fluorescence microscopy (LCFM) is a powerful tool used to investigate cellular dynamics in real time. (biomedcentral.com)
  • This method allows the examination of single-cell dynamics to be correlated with cellular stage and ploidy in a high-throughput fashion. (biomedcentral.com)
  • To look at if eupatorin can perturb spindle dynamics at M phase, we examined the cells' capability to convert the spindle architecture from monopolar to bipolar structure. (mirnamimics.com)
  • Greater than 95% knockdown of the non-catalytic subunit CSN2 and the deneddylating subunit CSN5 of the CSN was achieved in the human myeloid progenitor cell line K562. (biomedcentral.com)
  • Effects of 27 MHz radiation on somatic and germ cells. (cdc.gov)
  • Even within humans and other mortal species, there are cells with the potential for immortality: cancer cells which have lost the ability to die when maintained in a cell culture such as the HeLa cell line,[17] and specific stem cells such as germ cells (producing ova and spermatozoa). (stevechase.org)
  • The ease of motif acquisition simplifies access to basal housekeeping functions, facilitates the co-regulation of multiple biomolecules allowing them to respond in a coordinated manner to changes in the cell state, and supports the integration of multiple signals for combinatorial decision-making. (biomedcentral.com)
  • An animation that takes the viewer inside a mammalian cell where one can see the nucleus and its characteristic envelope connected to the endoplasmic reticulum. (cellimagelibrary.org)
  • In cell division, a cell makes a copy of its DNA and then separates itself into two identical cells - each with its own copy of DNA enveloped inside a nucleus. (visionlearning.com)
  • advanced organisms-including animals, plants, fungi, and protists-whose cells have a nucleus and other membrane-bound organelles. (visionlearning.com)
  • mostly one-celled organisms, bacteria for example, whose cells do not have a nucleus or other membrane-bound organelles. (visionlearning.com)
  • He began a series of live observations under the microscope using dyed samples of animal tissues and found that a particular mass of material inside the nucleus of cells absorbed the dye quite well. (visionlearning.com)
  • The nuclear lamina, which coats the inner nuclear cell membrane, represents a major repressive environment in the nucleus. (biomedcentral.com)
  • In this study, the miRNAs and their target mRNAs in the Argonaute2 (Ago2) complex of nucleus and cytoplasm of gastric cancer cells were characterized using high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP). (biomedcentral.com)
  • The target mRNAs in the Argonaute2 (Ago2) complex of nucleus and cytoplasm of gastric cancer cells were analyzed through Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) analysis. (biomedcentral.com)
  • microRNA-mRNA interactions occur in nucleus and cytoplasm of gastric cancer cells. (biomedcentral.com)
  • A PRT062607 HCL hallmark of RGP cell behaviour is the cell cycle-linked oscillatory movement of the nucleus of RGP cells termed interkinetic nuclear migration (INM)24. (biotech-angels.com)
  • The RGP nucleus then migrates basally during G1 progresses through S-phase in the upper portion of the VZ and then migrates apically during G2 toward the ventricle where PRT062607 HCL the next mitotic division occurs26. (biotech-angels.com)
  • Carcinogens are known to cause swelling of the mammalian cell nucleus. (biomedcentral.com)
  • These results strongly suggest that NS is mainly caused by structural aberrations in the nucleus during interphase of the cell cycle. (biomedcentral.com)
  • Carcinogens cause swelling of the mammalian cell nucleus, a phenomenon first reported by Page [ 1 ]. (biomedcentral.com)
  • A more compact fraction from the cells within the population had bipolar spindle with satellite rods (17%, 5 cells from 30). (mirnamimics.com)
  • Not surprisingly, control cells given MG132 had bipolar spindle with two pericentrinpositive centrosomes (90%, 27 cells from 30, Fig. 5A). (mirnamimics.com)
  • Relaxation from the cells within the population had bipolar spindle (59%, 19 cells from 32) however with several satellite rods. (mirnamimics.com)
  • To understand the role of autophagy in the pathogenesis of glial tumors in vivo, an established Drosophila melanogaster model of glioma was used based on overexpression in larval glial cells of an active human EGFR and of the PI3K homolog Pi3K92E /Dp110. (sdbonline.org)
  • Biochemical reconstitution demonstrates that the mammalian complex possesses inherent E3 ubiquitin ligase activity, using Ube2H as its cognate E2. (elifesciences.org)
  • Our biochemical and cellular analysis thus demonstrates that the GID/CTLH complex prevents cell cycle exit in G1, at least in part by degrading Hbp1. (elifesciences.org)
  • These assays can be either biochemical (cell-free) or cell-based. (oncotarget.com)
  • Cell types included resting human peripheral lymphocytes, LN71 or RT2 glioma cells, Chinese-hamster-ovary cells, HeLa cells, and mouse spermatozoa. (cdc.gov)
  • Together this signifies that exposure Bicalutamide recently G2 cells to eupatorin causesdefects in spindle formation.To review the result of eupatorin on spindle maintenance, we treated MG132 blocked metaphase cells with eupatorin for just two h within the ongoing presence of MG132. (mirnamimics.com)
  • 18] In artificial cloning, adult cells can be rejuvenated to embryonic status and then used to grow a new tissue or animal without ageing. (stevechase.org)
  • In addition studies on HeLa cells using Plk1 siRNA interference and overexpression showed that phosphorylation of RSK1 increased upon interference and decreased after overexpression suggesting that Plk1 inhibits RSK1. (biospraysehatalami.com)
  • gene was first identified during screening of for mutants defective in cell division. (biospraysehatalami.com)
  • NF-kB DNA binding activity is certainly reduced and its own focus on gene items, including MMP-9, survivin, IAP-1, BCL-xL, FLIP Tauroursodeoxycholate and TRAF1, are abrogated in GKS3-null cells [16]. (globaltechbiz.com)
  • MCPH is inherited in an autosomal recessive pattern in which both copies of the gene in each cell have mutations. (biomedcentral.com)
  • Taken together these findings provide insights into the regulation of Plk1 during cell division and diapause cyst formation and the correlation between the activity of Plk1 and RSK1. (biospraysehatalami.com)
  • Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. (lookformedical.com)
  • Clonogenic assays were performed to determine the ability of PC3 and HeLa cells to form colonies upon PI3K-C2β downregulation in the absence or presence of docetaxel. (biomedcentral.com)
  • Metastasis Assays 435s M14 cells were transfected with pcDNA EGFP N1, and pcDNA3.1 Zeo luciferase into pcDNA3.one Zeo, followed by zeocin G418 selection. (aktinhibitor.com)
  • Here we discuss the ATAD5-luciferase assay and expand upon the value of HTS in identifying other potential cancer drugs, focusing on cell-based assays that involve DNA damage or repair pathways. (oncotarget.com)
  • However, because they contain more than one target, cell-based assays may also require more complex secondary screens than HTS assays that use cell-free systems. (oncotarget.com)
  • In this manuscript, we discuss the various cell-based HTS assays that have been developed to aid in the discovery of chemotherapeutic agents, focusing on those that exploit the DNA damage response. (oncotarget.com)
  • we obtained additional information nearly simultaneously through additional microgram-scale sampling during the respective cell assays. (biomedcentral.com)
  • Cell division requires the tight coordination of multiple cytoskeletal pathways. (biologists.com)
  • This continues for 10 minutes after division before the daughter cells revert to normal random motility, indicating that this is a tightly regulated process. (biologists.com)
  • Most of the cells that make up higher organisms, like vertebrate animals and flowering plants, reproduce via a process called cell division. (visionlearning.com)
  • That's right, the hundreds of millions of cells that make up the bone and flesh of your body are products of thousands of generations of cell division that began when you were smaller than the period at the end of this sentence. (visionlearning.com)
  • In particular, he was interested in the process of cell division. (visionlearning.com)
  • All of the processes involved in increasing CELL NUMBER including CELL DIVISION . (lookformedical.com)
  • NuMA in rat testis - Evidence for roles in proliferative activity and meiotic cell division. (mpg.de)
  • Traditionally, reporting DNA content has been accomplished with a high degree of accuracy in large cell populations using flow cytometry, a technique that allows the proportion of cells in each phase of the cell cycle to be calculated in a high-throughput fashion. (biomedcentral.com)
  • Two daughter cells are the result of the mitotic process while four cells result from the meiotic process. (stevechase.org)
  • In travsverse sections of midbodies from HeLa and PtK cells, we find that essentially all the MTs in a given region of the structures have the same direction of hook curvature, and hence the same polarity. (rupress.org)
  • The systematic and reproducible polarities observed favor the concept that MT polarity is an important factor in the formation and/or the function of these two mitotic structures. (rupress.org)
  • The effect was that different parts of a cell would absorb more dye, in effect "highlighting" them, as in Figure 3, to reveal structures and processes that were invisible before. (visionlearning.com)
  • Effects of sinusoidal magnetic field observed on cell proliferation, ion concentration, and osmolarity in two human cancer cell lines med. (emf-portal.org)
  • 1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
  • In addition, computer programs usually predict targets on a genome-wide scale irrespective of mRNA and miRNA that are specific to tissues or cell lines. (biomedcentral.com)
  • Human breast cancer cell lines, MCF7 and MDA MB 231 were cultured at 37 C in Dulbeccos Modified Eagle Medium supplemented with 100 units/ml penicillin, 10 % fetal bovine serum and 100 g/ml streptomycin in a moist incubator with 5% CO2. (ilreceptor.com)
  • Subsequently, GSK3 promotes oncogene-induced change and proliferation in leukemia cell lines. (globaltechbiz.com)
  • Immunocytochemical analysis of cell lines derived from solid tumors. (mpg.de)
  • Although studied in model human cell lines such as HeLa, very little is known about the role of the CSN in haemopoietic cells. (biomedcentral.com)
  • Republican National Committee Normal human cells however die after about 50 cell divisions in laboratory culture (the Hayflick Limit, discovered by Leonard Hayflick in 1961). (stevechase.org)
  • The ability to measure DNA content in single cells by means of LCFM would allow cellular stage and ploidy to be coupled with a variety of imaging directed analyses. (biomedcentral.com)
  • Its quantification serves to assess several cellular parameters including DNA ploidy and a cell's temporal location within the cell cycle [ 1 ]. (biomedcentral.com)
  • To overcome this limitation, approaches incorporating laser scanning cytometry and fluorescence microscopy have been successful in quantifying DNA content in single cells [ 2 , 3 ], providing the capacity to combine this information with additional cellular parameters that can be elucidated by imaging. (biomedcentral.com)
  • Therefore, traditional methods for examining DNA content by microscopy have relied on cellular fixation, which is incompatible with tracking cells over time. (biomedcentral.com)
  • Our findings claim that the book GSK3 inhibitor, Tauroursodeoxycholate CG0009, inhibits breasts cancers cell development through cyclin D1 p53 and depletion activation, and may hence offer a forward thinking healing approach for breasts malignancies resistant to hormone-based therapy. (globaltechbiz.com)
  • Studies were also conducted on liposome permeability, and a mathematical model was used to calculate radiation energy absorption in mammalian cell models. (cdc.gov)
  • Numerous studies have evaluated nuclear swelling (NS), including in cultured mammalian cells (e.g. (biomedcentral.com)