Mitochondrial dna probes. Medical search. Frequent questions

The mtDNA control region is an area of the mitochondrial genome which is non-coding DNA. (wikipedia.org)
By using 2 segments of mitochondrial DNA (mtDNA) hypervariable segment I (HVS-I) target, we determined the ancestry of the humans from whom the bones were derived ( 4 ). (cdc.gov)
In multiple species, certain tissue types are prone to acquiring greater loads of mitochondrial genome (mtDNA) mutations relative to others, however the mechanisms that drive these heteroplasmy differences are unknown. (biorxiv.org)
The composition of the mitochondrial DNA (mtDNA) in each cell is in constant flux through processes of mutation, replication, and degradation. (biorxiv.org)
Variants within the high copy number mitochondrial genome (mtDNA) can disrupt organelle function and lead to severe multisystem disease. (jci.org)
The wide range of manifestations observed in patients with mitochondrial disease results from varying fractions of abnormal mtDNA molecules in different cells and tissues, a phenomenon termed heteroplasmy. (jci.org)
Here, we identify nonrandom distribution of a pathogenic mtDNA variant across a complex tissue using single-cell RNA-Seq, mitochondrial single-cell ATAC sequencing, and multimodal single-cell sequencing. (jci.org)
Mitochondrial DNA (mtDNA) is a small circular double-stranded DNA molecule that contains information that is essential for efficient energy production. (umu.se)
Long an important and useful tool in forensic genetic investigations, mitochondrial DNA (mtDNA) typing continues to mature. (ojp.gov)
Although its precise biological function remains unclear, its proximity to mitochondrial DNA (mtDNA) makes it an excellent candidate to participate in mtDNA replication, metabolism and maintenance. (mdpi.com)
Lewis uses quantitative imaging, genetics and systems biology approaches to study mitochondrial DNA in order to understand how mtDNA copy number is regulated within and across cell cycles, and how the disruption of these pathways impinges on mtDNA integrity, cellular metabolism and ultimately human health. (berkeley.edu)
Not only did curcumin treatment increase mitochondrial DNA (mtDNA) copy number in hepatocytes, but it also increased levels of transcriptional factors that regulate mitochondrial biogenesis, including peroxisome proliferator-activated receptor-γ coactivator 1α (PGC1α), nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (Tfam). (spandidos-publications.com)
Hepatocellular lipid accumulation is thought to simultaneously stimulate mitochondrial fatty acid oxidation and the production of ROS, thereby promoting lipid peroxidation and damage of mitochondrial DNA (mtDNA) ( 3 ). (spandidos-publications.com)

D-loop Hypervariable region Structure of the Mitochondrial Genome DNA Learning Center, Cold Spring Harbor Laboratory Stoneking M, Hedgecock D, Higuchi RG, Vigilant L, Erlich HA (February 1991). (wikipedia.org)
We found that the conserved PTEN-induced putative kinase (PINK1/PINK-1) and the E3 ubiquitin-protein ligase parkin (PDR-1), which are required for mitochondrial autophagy (mitophagy), underlie stereotyped differences in heteroplasmy of a deleterious mitochondrial genome mutation (ΔmtDNA) between major somatic tissues types in Caenorhabditis elegans . (biorxiv.org)
Research in the last few years has demonstrated both that data from the entire molecule will have practical benefits in forensic DNA casework, and that massively parallel sequencing (MPS) methods will make full mitochondrial genome (mtGenome) sequencing of forensic specimens feasible and cost-effective. (ojp.gov)
DNA microarrays can be used in genome-wide association studies (GWAS) to compare patient and control populations to identify DNA variants that can contribute to disease risk. (msdmanuals.com)
Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)
DNA from a patient is compared to a reference genome using many oligonucleotide probes. (msdmanuals.com)

There is also growing evidence that FFA-mediated oxidative stress contributes significantly to mitochondrial dysfunction in the liver. (spandidos-publications.com)
New findings suggest that oxidative stress damages mitochondrial DNA, and they link this damage to a disease state. (vanderbilt.edu)

Inside mitochondria resides a unique subset of the cell's DNA that must be organized and condensed correctly to sustain normal function. (news-line.com)
They found that when TFAM levels are reduced, mitochondrial DNA is expelled from the mitochondria into the cell's interior. (news-line.com)
Remarkably, low levels of DNMT3A and TET2 expression in blood cells leads to reduced TFAM expression, which in turn leads to abnormal mitochondria DNA packaging, instigating inflammation due to released mitochondrial DNA. (news-line.com)
The HVR1 and HVR2 regions are tested at most testing companies, and at Family Tree DNA, the rest of the mitochondria, called the coding region, is tested as well with the full mitochondrial sequence test . (dna-explained.com)
Ever since we revealed this pathway, there has been an explosion of interest in mitochondria being involved in inflammation and many reports linking mitochondrial DNA release to other clinical contexts. (businessremark.com)
Mitochondrial Membrane Potential Mitochondrial membrane potential () was evaluated with tetraethyl benzimidazolyl carbocyanine iodide cationic dye, which exhibited potential\reliant deposition in mitochondria, producing a fluorescence emission change from 525?nm (green) to 590?nm (crimson). (cancer-pictures.org)

Mitochondrial function or the ability to generate energy through OXPHOS (oxidative phosphorylation) is vital for cell homeostasis and its dysfunction has been linked to the pathogenesis of nearly all chronic diseases ( Pieczenik and Neustadt, 2007 ). (frontiersin.org)
Relevant to the studies presented in this manuscript, mitochondrial dysfunction is a key contributor to the perpetuation of metabolic inflammation caused by nutrient excess. (frontiersin.org)
Besides OXPHOS, mitochondrial dysfunction is also present in the examination of mitochondrial morphology, the balance of mitochondrial network formation, and fission and fusion. (frontiersin.org)
Insulin resistance occurs in almost all patients with non-alcoholic fatty liver disease (NAFLD), and mitochondrial dysfunction likely plays a pivotal role in the progression of fatty liver into non-alcoholic steatohepatitis (NASH). (spandidos-publications.com)
Together, these data suggest that curcumin protects hepatocytes from HFFA-induced lipoapoptosis and mitochondrial dysfunction, which partially occurs through the regulation of mitochondrial biogenesis. (spandidos-publications.com)
Therefore, designing therapies that prevent mitochondrial dysfunction stands to be one of the most important strategies in for treating of NAFLD and its complications. (spandidos-publications.com)
Many studies implicate mitochondrial dysfunction as a key contributor to cell loss in Parkinson disease (PD). (nature.com)

The new "spider‐specific" probes were used to sequence UCE loci in 51 specimens. (researchgate.net)
DNA probes, complementary to primarily to rRNA and select mitochondrial sequences host sequence, can be used to degrade host RNA using RNAse H treatment. (usda.gov)
We used 40 ± 5 nm gold nanoparticles (GNPs) as colorimetric sensor to visually detect swine-specific conserved sequence and nucleotide mismatch in PCR-amplified and non-amplified mitochondrial DNA mixtures to authenticate species. (upm.edu.my)
Since 1993 when the function of a non-coding DNA sequence was first discovered in the model organism Caenorhabditis elegans, many microRNAs have been implicated in having a central role in numerous physiological and pathological cellular processes. (edu.au)
Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA from two parasitic worms - Trichinella spiralis (partial sequence of 18S rRNA gene) and Taenia saginata (partial sequence of mitochondrial COX1 gene) were designed. (muni.cz)
Therefore, we investigated the 17 Yfiler Y-STRs in 1,730-1,764 DNA-confirmed father-son pairs per locus and found 84 sequence-confirmed mutations among the 29,792 meiotic transfers covered. (springer.com)
Different types of probes may investigate a broad range of sizes of DNA sequence. (msdmanuals.com)
The transporter was identified by probing a mammalian-expressed sequence tag database with 2 fungal mitochondrial ornithine carrier protein sequences. (medscape.com)
PCR (polymerase chain reaction) technique has achieved increased importance for post-mortem DNA analysis in forensic cases 8 because of the millions of copies amplified from one specific sequence of DNA. (bvsalud.org)

Mitochondrial DNA with multiple different deletions shows clonal expansion, which leads to mitochondrial myopathy with ragged-red fibers and muscle weakness. (nih.gov)
Missing or reducing this binding activity leads to mitochondrial DNA release and an overactive mitochondrial inflammation response, and we believe this may exacerbate plaque buildup in atherosclerosis. (news-line.com)

Heteroplasmy and mosaicism are therefore important determinants of mitochondrial disease pathophysiology. (biorxiv.org)
We profiled the transcriptome, chromatin accessibility state, and heteroplasmy in cells from the eyes of a patient with mitochondrial encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) and from healthy control donors. (jci.org)

The tagged DNA binds to its complementary DNA segment and can be detected by measuring the amount and type of fluorescence. (msdmanuals.com)
Ornithine incorporation was restored in fibroblasts derived from patients with hyperornithinemia-hyperammonemia-homocitrullinuria syndrome by transforming the fibroblasts with transporter complementary DNA (cDNA). (medscape.com)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (bvsalud.org)

Long-term screening for primary mitochondrial DNA variants associated with Leber Hereditary Optic Neuropathy: incidence, penetrance and clinical features. (cdc.gov)

We studied several members of a Swedish family with autosomal dominant progressive external ophthalmoplegia and multiple mitochondrial DNA deletions by polymerase chain reaction analysis of single muscle fibers and by in situ hybridization, combined with enzyme histochemical analysis. (nih.gov)
Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)
The nuclear and mitochondrial analyses together suggest that the common ancestor of the wisent and CladeX mitochondrial lineages originated from asymmetrical hybridization (or sustained introgression) between male steppe bison and female aurochs. (slashgear.com)
We described the karyotypes of Dendropsophus seniculus (Cope, 1868), D. soaresi (Caramaschi & Jim, 1983) and D. novaisi (Bokermann, 1968) based on Giemsa staining, C-banding, silver impregnation and in situ hybridization with telomeric probes. (pensoft.net)
Hybridization-based target enrichment panel with our intrinsic probe design, rebalancing, and molecular barcode technologies to efficiently analyze not only hard-to-capture areas but also challenging samples, such as damaged DNA or RNA. (celemics.com)
Hybridization of a 27-nucleotide probe to swine mitochondrial DNA identified them in pork-venison, pork-shad and venison-shad binary admixtures, eliminating the need of PCR amplification. (upm.edu.my)

We found that the genes DNMT3A and TET2, in addition to their normal job of altering chemical tags to regulate DNA, directly activate expression of a gene involved in mitochondrial inflammatory pathways, which hints at a new molecular target for atherosclerosis therapeutics," says Gerald Shadel, co-senior author, Salk professor and director of the San Diego Nathan Shock Center of Excellence in the Basic Biology of Aging. (news-line.com)
Previous analyses of dopaminergic (DAergic) neurons from patients with Lewy-body pathology revealed a deficiency in nuclear-encoded genes for mitochondrial respiration, many of which are targets for the transcription factor estrogen-related receptor gamma ( Esrrg /ERRγ). (nature.com)
While ERRγ deletion did not fully recapitulate the transcriptional alterations observed in postmortem tissue, it caused reductions in genes involved in synaptic and mitochondrial function and autophagy. (nature.com)
Further evidence suggests that during PD progression, transcriptional programs for the expression of genes involved in mitochondrial structure and function are disrupted 11 . (nature.com)
Laser-captured microdissected (LCM) DAergic neurons from postmortem tissue of patients with Lewy-pathology revealed a deficiency in expression for nuclear-encoded genes involved in mitochondrial respiration and function 12 . (nature.com)
in fact, these genes are targets of the transcriptional coactivator and master regulator of transcription of mitochondrial genes peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) 12 . (nature.com)
Direct regulators of nuclear-encoded mitochondrial genes are members of the estrogen-related receptor (ERR) family, encoded by Esrra , Esrrb and Esrrg . (nature.com)
Los ARNs antisentido sintéticos se utilizan para afectar el funcionamiento de genes específicos en la investigación o con fines terapéuticos. (bvsalud.org)

In addition, it improved mitochondrial biogenesis by peroxisome proliferatorCactivated receptor\ coactivator 1/nuclear respiratory aspect\1/mitochondrial transcription aspect A signaling governed by adiponectin/AMP\turned on proteins kinase. (cancer-pictures.org)

We discuss amplicon-based DNA barcoding and metabarcoding for the analysis of community diversity and molecular gut content analysis for assessing predator-prey relationships. (springer.com)
I lead a research group that focuses on mitochondrial DNA and the molecular mechanisms that mediate the signaling of its instability. (umu.se)
A thorough understanding of the molecular mechanisms that mediate these signals relates to various hereditary mitochondrial diseases, but also to neurodegenerative disease and various types of cancers. (umu.se)
After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. (bvsalud.org)
Daniel Topgaard Molecular motion probed by nuclear spin relaxation and diffusion experiments. (lu.se)

I am binding genomic DNA from _E.coli_, Yeast, and _Methanococcus maripaludis_ (Archaea) to the membrane using baking at 80¡C for 2 hours. (bio.net)
The membrane was stripped of probe using a NaOH treatment and neutralized with TE buffer (pH 7.5). (bio.net)
The same membrane was then hybridized with the Bacterial-specific probe. (bio.net)
Exposure of the membrane between hybridizations showed most of the universal probe stripped, so that probably wasn't the problem. (bio.net)
In addition, curcumin contributed to cell survival, as indicated by the restoration of the mitochondrial membrane potential (MMP) and the inhibition of the mitochondrial biogenesis induced by HFFA. (spandidos-publications.com)
price, PF-04691502 IC50 avoided mitochondrial membrane depolarization, and alleviated mitochondrial bloating in diabetic rabbits. (cancer-pictures.org)
In the present review, we outline commonly used methods that are based on the assessment of mitochondrial transmembrane potential, activation of caspases, DNA fragmentation, and plasma membrane alterations. (edu.au)
An inner mitochondrial membrane transporter directs ornithine to the transcarbamoylase enzyme to keep intramatrix ornithine levels low. (medscape.com)
This transport of ornithine across the inner mitochondrial membrane is essential to the urea cycle. (medscape.com)

The mitochondrial isolation techniques were finished within 1?hour following the rabbits were euthanized. (cancer-pictures.org)
Currently, the calibration of MAGPIX system is in process together with arranging procedure for preparing the sample - from election of the most suitable input material to DNA isolation. (muni.cz)

new data about glycolytic increase and mitochondrial rearrangements suggest pathways leading to cell transformation. (unimib.it)

Shadel's team previously investigated the effects of mitochondrial DNA stress by removing TFAM, a gene that helps ensure mitochondrial DNA is packaged correctly. (news-line.com)
Collectively, our data indicate that MSC therapy rescues impaired mitochondrial morphology, enhances host metabolic capacity, and induces widespread host gene shifting. (frontiersin.org)
We demonstrate that deletion of ERRγ from DAergic neurons in adult mice was sufficient to cause a levodopa-responsive PD-like phenotype with reductions in mitochondrial gene expression and number, that partial deficiency of ERRγ hastens synuclein-mediated toxicity, and that ERRγ overexpression reduces inclusion load and delays synuclein-mediated cell loss. (nature.com)
A small amount of DNA can be amplified using the polymerase chain reaction (PCR) process, which can produce millions of copies of a gene or gene segments. (msdmanuals.com)
Overview of Genetics A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)
Gene probes can be used to locate specific segments of normal or mutated DNA. (msdmanuals.com)
Gene probes can detect a number of disorders before and after birth. (msdmanuals.com)
Safety of Intravitreal Gene Therapy for Treatment of Subjects with Leber Hereditary Optic Neuropathy due to Mutations in the Mitochondrial ND4 Gene: The REVEAL Study. (cdc.gov)

We compared the performance of stool microscopy with the highly specific real-time polymerase chain reaction (PCR) we recently described for the detection and quantification of parasite-specific DNA. (cdc.gov)
The survival of grafted cardiomyocytes was confirmed by observing the fluorescent cell tracer Vybrant™ CM-DiI or expression of the enhanced green fluorescent protein (eGFP) in transplanted cells, or survival was demonstrated by polymerase chain reaction (PCR)-based detection of human mitochondrial DNA. (biomedcentral.com)

Conclusions Dipeptidyl peptidase\4 inhibitors can prevent atrial fibrillation by reversing electrophysiological abnormalities, enhancing mitochondrial function, and marketing mitochondrial biogenesis. (cancer-pictures.org)
The dichlorodihydrofluorescein diacetate fluorescence was normalized towards the fold from the control group.21 American Blot Analysis American blotting was performed to measure the expression of proteins on mitochondrial biogenesis in 3 groups. (cancer-pictures.org)

2022. Probing the mechanisms of two exonuclease domain mutators of DNA polymerase ε. (nih.gov)
2022. Ribonucleotide incorporation by eukaryotic B-family DNA replicases and its consequences. (nih.gov)

The problem was we couldn't work out how DNMT3A and TET2 were involved because the proteins they code do seemingly opposite things regarding DNA regulation," says Christopher Glass, co-senior author and professor at the UC San Diego School of Medicine. (news-line.com)
Mitochondrial proteins articles was assayed utilizing a BSA proteins assay reagent package (Thermo Scientific). (cancer-pictures.org)
Within a 3\mL shut thermostatic Ly6a and magnetically stirred cup chamber, respiration moderate (mannitol 225?mmol/L, sucrose 70?mmol/L, EDTANa2 1?mmol/L, KH2PO4 20?mmol/L, K2HPO4 20?mmol/L, and BSA 1?mg/mL, PH 7.4) was saturated with ambient air to attain a focus of 258?mol/L. After an equilibration period, 300?g of mitochondrial proteins was put into the reaction program. (cancer-pictures.org)
Therefore, lack of was detectable with the reduction in the crimson to green fluorescence emission proportion.19 The tests had been conducted at 25C in 2?mL of respiration moderate with 300?g of mitochondrial proteins, and tetraethyl benzimidazolyl carbocyanine iodide dye equilibration was allowed for 10?a few minutes. (cancer-pictures.org)
Mitochondrial proteins 300?g was put into a quartz cuvette containing 3?mL of phosphate buffer (KCl 130?mmol/L, MgCl2 43?mmol/L, NaH2PO4 20?mmol/L, blood sugar 30?mmol/L, malate 2?mmol/L, and PH 7.4) and 2?L of 2.5?mmol/L dichlorodihydrofluorescein diacetate, that was dissolved in 1.25?mmol/L methanol and kept at night in 0C. (cancer-pictures.org)
As with other mitochondrial carrier family proteins, the ornithine carrier is composed of 300 amino acids that constitute 3 repeated motifs of approximately 100 amino acids each. (medscape.com)

The probes themselves are domain specific for Universal, Eucarya, Bacteria, and Archaeal sequences in the 16S rDNA. (bio.net)
As expected, the E.coli showed a linear pattern of binding, the Yeast showed some low binding (presumably due to the mitochondrial DNA), however the spotted archaeal sequences produced some strange results. (bio.net)
This approach will have diminishing returns because of the cost of adding additional sequences to the probe mix, but this approach will likely provide an increase in sensitivity for viral sequences. (usda.gov)
Not only animals from the same order (fish, amphibians, reptiles, mammals, etc.) must be taken in account during primer/probe design, but all known DNA sequences must be checked for nonspecific binding of the primers and probe. (sylphium.com)

MSC co-culture with skin fibroblasts from mitochondrial disease patients rescued aberrant mitochondrial morphology from a fission state to a more fused appearance indicating an effect of MSC co-culture on host cell mitochondrial network formation. (frontiersin.org)

In the present study, we examine the specific impacts of MSCs on mitochondrial morphology and function in host tissues. (frontiersin.org)
At present, little is known about the impact of MSC therapy on mitochondrial function and morphology. (frontiersin.org)
DNA damage was evaluated by Comet assay, and mitochondrial morphology was analyzed in confocal microscopy. (unimib.it)

Celemics provides comprehensive solution for DNA methylation sequencing with proprietary probes specifically designed for methyl-seq, enabling accurate detection of methylation sites. (celemics.com)

For this purpose, we adopted high sensitive multiplex oligonucleotide ligation- PCR assay - MOL-PCR, enabling direct and simultaneous detection of multiple nucleic acid signatures from complex mixtures potentially containing the DNA originating from number of different parasitic organisms and xMAP technology representing the novel platform based on magnetic microspheres. (muni.cz)

It's very exciting to see our discovery on TFAM depletion causing mitochondrial DNA stress and inflammation now has direct relevance for a disease like atherosclerosis," says Shadel, who holds the Audrey Geisel Chair in Biomedical Science. (news-line.com)

Title: Versatile Association Behavior in Mixtures of Oppositely Charged Amphiphiles: From DNA-Like Assembly of Supramolecular Helices to Coacervation in Chiral Surfactant Systems. (lu.se)

The LOD (for genomic DNA) of the assay was 6 µg ml − 1 swine DNA in mixed meat samples. (upm.edu.my)

Our results indicate that a nuclear factor predisposes to the development of somatic multiple mitochondrial DNA deletions. (nih.gov)

An imaging probe developed at Vanderbilt detects retinal inflammation early and may allow therapeutic intervention to prevent blindness. (vanderbilt.edu)

Sporadic progressive external ophthalmoplegia and Kearns-Sayre syndrome are usually associated with single large-scale mitochondrial DNA deletions in muscle. (nih.gov)
In progressive external ophthalmoplegia with autosomal dominant inheritance, multiple mitochondrial DNA deletions have been reported. (nih.gov)
Muscle fiber segments with deficiency of cytochrome c oxidase, which is partially encoded by mitochondrial DNA, had accumulated mitochondrial DNA with deletions and showed reduced levels of wild-type mitochondrial DNA. (nih.gov)
Young, clinically affected individuals of this family without limb muscle symptoms did not show mitochondrial DNA deletions or cytochrome c oxidase-deficient muscle fibers. (nih.gov)

Mitochondrial respiratory system function was initiated with a 15\L combination of 0.8?mol/L malic acidity and 1?mol/L glutamic acidity, as well as the alteration from the fluorescence emission was detected. (cancer-pictures.org)

In vivo experiments confirmed mitochondrial abundance and mitochondrial oxygen consumption rates were elevated in host tissues following MSC treatment. (frontiersin.org)

Scientists from the Glass and Shadel labs worked together to better understand why DNMT3A and TET2 mutations led to inflammation responses similar to those observed during mitochondrial DNA stress. (news-line.com)
Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)

Optimization of a genotyping screening based on hydrolysis probes to detect the main mutations related to Leber hereditary optic neuropathy (LHON). (cdc.gov)

A total of 5 enzymes in 2 subcellular compartments (mitochondrial matrix and cytosol) convert ammonia into urea, which is excreted by the kidney (see image below). (medscape.com)

Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. (bvsalud.org)

Mitochondrial Respiration Mitochondrial respiratory function was assessed polarographically at 25C PF-04691502 IC50 utilizing a Clark\type air electrode (Oroboros Equipment). (cancer-pictures.org)
Upon stabilization from the mitochondrial air intake, a 15\L combination of 0.8?mol/L malic acidity and 1?mol/L glutamic acidity was put into initiate the condition 2 respiration. (cancer-pictures.org)

Analyses of the 'halos' showed that the probe did not bind in a linear manner. (bio.net)
In recent years, large-scale community analyses have benefitted tremendously from advances in DNA barcoding technology. (springer.com)
High-throughput sequencing (HTS), particularly DNA metabarcoding, enables community-wide analyses of diversity and interactions at unprecedented scales and at a fraction of the cost that was previously possible. (springer.com)
In the last two decades, DNA barcoding, the sequencing of short species-specific amplicons, has considerably simplified community analyses (Hebert et al. (springer.com)

Mitochondrial Reactive Air Species Production Regarding to a report by Bo et?al,20 mitochondrial reactive oxygen species (ROS) generation PF-04691502 IC50 was assessed using clean mitochondrial suspensions using the dichlorodihydrofluorescein diacetate probe. (cancer-pictures.org)

Benjamin Safdi , assistant professor of physics, who investigates the microscopic nature of dark matter using novel laboratory experiments and astrophysical probes. (berkeley.edu)

2003 ). DNA barcodes can provide information on genetic variation within and between species, rapidly assign taxonomic status across divergent lineages (Hebert and Gregory 2005 ), and identify the prey composition of predators' gut contents (Agustí et al. (springer.com)

He and I agree that it might be more telling to search stored samples for mitochondrial DNA that could reveal the species of kidney cells used. (aidsorigins.com)
Variety of cytometric methods designed to identify apoptotic cells, detect particular events of apoptosis and probe mechanisms associated with this mode of cell death have been developed during the past two decades. (edu.au)

Highest possible sensitivity (1 DNA copy per reaction). (sylphium.com)

Teeth have in their composition the most resistant tissue in the human body - dental enamel - which provides high resistance against adverse conditions that can degrade DNA in the whole dental structure 14 . (bvsalud.org)

While we have a tip calculator at Family Tree DNA for Y-line DNA to predict how long ago 2 Y-line matches shared a most recent common ancestor , we don't have anything similar for mitochondrial DNA , partly because of the great variation in the mutation rates for the various regions of mitochondrial DNA. (dna-explained.com)
As a postdoc with Michael Wigler (2004) and independent Cold Spring Harbor Laboratory Fellow (2004-2007), Dr. Hall used microarray technologies and mouse strain genealogies to conduct the first systematic study of DNA copy number variation hotspots. (yale.edu)

Problems with mitochondrial DNA can therefore lead to a cellular energy defect and mitochondrial disease. (umu.se)
In this syndrome, a defect in the transport of ornithine into the mitochondrial matrix significantly inhibits the urea cycle, thereby impeding nitrogen disposal. (medscape.com)

In forensics, mutation rates are needed when STRs are applied to paternity testing, and Y-STRs are especially powerful in deficiency cases of disputed paternity involving male offspring where the alleged father is not available for DNA analysis but is replaced by any of his male paternal relatives. (springer.com)
Of these, studying DNA-confirmed father-son pairs is the most reliable approach but only if the number of father-son pairs investigated is large enough to reveal reliable mutation rate estimates. (springer.com)

aDNA hybridizations with MTC probes were conducted as described ( 4 ). (cdc.gov)

Anatomy13

Organisms10

Diseases15

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes65

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena3

Humanities1

Information Science4

Health Care3

Geographicals5

MtDNA13

Genome6

Oxidative stress2

Mitochondria6

Dysfunction7

Sequence9

Leads to mitochondrial2

Heteroplasmy2

Complementary3

Variants1

Hybridization6

Genes8

Peroxisome1

Molecular5

Membrane9

Isolation2

Pathways1

Gene8

Polymerase Chain Re2

Biogenesis2

20222

Proteins6

Sequences4

Fibroblasts1

Morphology3

Methylation1

Detection1

TFAM1

Mixtures1

Assay1

Somatic1

Retinal1

Deletions4

Fluorescence1

Tissues1

Inflammation2

Hereditary1

Compartments1

Genomic1

Respiration2

Analyses4

Reactive1

Experiments1

Genetic1

Cells2

Reaction1

Tissue1

Variation2

Defect2

Mutation2

Hybridizations1