Mitochondrial cell membrane potentials. Medical search. Frequent questions

Green husk extract generates an antiproliferative effect in HL-60 cells, which is related to an induction of the early stages of apoptosis and a loss of mitochondrial membrane potential. (ejbiotechnology.info)
We offer a range of products that detect apoptotic cells through the measurement of active caspases and loss of mitochondrial membrane potential. (biosciregister.com)
Li HL, Chen DD, Li XH, Zhang HW, Lü JH, Ren XD, Wang CC. JTE-522-induced apoptosis in human gastric adenocarinoma cell line AGS cells by caspase activation accompanying cytochrome C release, membrane translocation of Bax and loss of mitochondrial membrane potential. (wjgnet.com)
Synaptic NMDA receptors have anti-apoptotic activity, whereas stimulation of extrasynaptic NMDA receptors caused loss of mitochondrial membrane potential (an early marker for glutamate-induced neuronal damage) and cell death. (nih.gov)

Further, cell death effect of ST was associated with induction of apoptosis. (biomedcentral.com)
A distinctive feature of the early stages of apoptosis is the disruption of normal mitochondrial function. (stratech.co.uk)
Jurkat cells treated by apoptosis inducing reagent, Staurosporine, were stained with JC-1 MitoMP Detection Kit. (dojindo.com)
The study also showed that when the pancreatic cancer cells were doubly assaulted - pre-treated with the antioxidant, resveratrol, and irradiated - the combination induced a type of cell death called apoptosis, an important goal of cancer therapy. (scienceblog.com)
They evaluated the mitochondria function of the cells treated with resveratrol, and also measured apoptosis (cell death), the level of reactive oxygen species in the cells, and how the cell membranes responded to the antioxidant. (scienceblog.com)
Immunohistochemical analysis revealed that tumor cell proliferation and apoptosis were higher in irradiated groups compared to the control group. (bioone.org)
Melatonin inhibited cell growth and induced apoptosis in 5RP7 cells. (tubitak.gov.tr)
These findings show that melatonin induces cell death and apoptosis in H-ras oncogene transformed 5RP7 cells. (tubitak.gov.tr)
Diminution of p53 by RNA interference induced necrosis instead of apoptosis in A549 cells following terpinen-4-ol treatment, indicating that terpinen-4-ol-elicited apoptosis is p53-dependent. (hindawi.com)
Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC. (hindawi.com)
Recent reports have indicated that terpinen-4-ol exerts its antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in mouse mesothelioma and melanoma cell lines without affecting normal cells [ 14 , 15 ]. (hindawi.com)
Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)
These endpoints can include membrane integrity, mitochondrial function, proliferation, and apoptosis versus necrosis. (nature.com)
Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
Further, AMP-17 treatment resulted in an increase in reactive oxygen species (ROS) levels, depolarization of the mitochondrial membrane potential (MMP), and changes in the cell cycle, leading to the apoptosis and necrosis, which ultimately contributed to the death of C. albicans cells. (researchsquare.com)
Human renal proximal convoluted tubule cell (HK-2) cultures were exposed to CNPs for 0, 12 and 72 h, and production of reactive oxygen species (ROS), mitochondrial membrane potential and apoptosis levels were evaluated. (nih.gov)
Transmission electron microscopy showed that CNPs can enter the cell by phagocytosis, and micrographs revealed signs of apoptosis and autophagy. (nih.gov)
CNPs also up-regulated expression of LC3-B, Beclin-1and p-JNK.CNPs are phagocytosed by HK-2 cells, leading to autophagy, apoptosis and ROS production, in part through activation of JNK signaling pathways. (nih.gov)
Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
Positively charged CTAC could actively target mitochondria by interacting with the negatively charged mitochondria membrane, and then lead to the dysfunction of mitochondria by decreasing mitochondrial potential and intracellular ATP levels, resulting in the necrosis and apoptosis of MCF-7 cells. (rsc.org)
In vitro exposure of human lymphocytes to 900 MHz CW and GSM modulated radiofrequency: studies of proliferation, apoptosis and mitochondrial membrane potential med. (emf-portal.org)
The first hour of exposure took place immediately after cell seeding and treatment with mitogenic or apoptosis inducers, the second and third hours took place after 24 or 48 h of culture . (emf-portal.org)

We offer a wide assortment of fluorescent probes for analyzing aspects of normal mitochondrial activity in live cells, including reactive oxygen species (ROS) production, mitochondrial membrane potential and calcium flux. (stratech.co.uk)
Mechanistically, both peptides caused a significant reduction at G0/G1 phase, in correlation with an augmented expression of the cell cycle inhibitory proteins p53, p27, p21, necrosis, exacerbated generation of reactive oxygen species and diminished mitochondrial membrane potential, all hallmarks of cellular stress. (nature.com)
Triggered the production of reactive oxygen species (ROS), which are substances circulating in the human body that have been implicated in a number of diseases: when ROS is increased, cells burn out and die. (scienceblog.com)
On the one hand, reactive oxygen species (ROS) are mandatory mediators for essential cellular functions including the function of germ cells (oocytes and spermatozoa) and thereby the fertilization process. (hindawi.com)

Learn about the assays used to assess cell proliferation and the cell cycle. (abcam.com)
We showed inhibition of cell proliferation that was time- and dose-dependent in 5RP7 cells for 24 and 48 h. (tubitak.gov.tr)
Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
1 It has become increasingly evident that mitochondria play a key role in the generation, proliferation and metastasis of cancer cells. (rsc.org)

A collapse in mitochondrial membrane and redox potential may induce unwanted loss of cell viability and be a cause of various pathologies. (stratech.co.uk)
Learn about cell viability assays. (abcam.com)
In addition, we designed and screened a panel of gomesin analogues with amino acid modifications that were predicted to influence cell viability. (nature.com)
The application of established cell viability assays such as the commonly used trypan blue staining method to coral cells is not straightforward due to different culture parameters and different cellular features specific to mammalian cells compared to marine invertebrates. (nature.com)
This work presents the approach used to customize dye pairs for membrane integrity-based cell viability assays considering the species- and genotype-specific autofluorescence of scleractinian corals, namely: endogenous fluorescence characterization followed by the selection of dyes that do not overlap with endogenous signals. (nature.com)
Table 1 includes common fluorophores used to assess membrane integrity and distinguish between live and dead cells when conducting viability assays. (nature.com)
they were found to contain fetuin-A. Exposure of HK-2 cells to CNPs induced ROS production, decreased mitochondrial membrane potential and decreased cell viability. (nih.gov)
The R123/Pl assay provides a quantitative measurement of mitochondrial membrane potential (cell motility) and cell membrane viability. (purdue.edu)
The normal cells were not affected when juglone is present at concentrations of 1 μM, while at higher concentrations, there is loss of viability of both cancerous and healthy cells. (ejbiotechnology.info)

Rupture of outer mitochondrial membrane (OMM) and ultimately cell death due to necrosis are the end results. (news-medical.net)

ST also caused the depolarization of mitochondrial membrane potential and increased Bax/Bcl-2 protein ratio. (biomedcentral.com)
This increases release of ACh after nerve impulses and may decrease rates of repolarization and depolarization of muscle cell membranes. (medscape.com)

A drastic rise in permeability of inner mitochondrial membrane (IMM), through consistent opening of a multi-protein channel referred to as the mitochondrial permeability transition pore (MPTP) results in mitochondrial dysfunction. (news-medical.net)
Retrieved on December 11, 2023 from https://www.news-medical.net/whitepaper/20180614/Inhibition-of-Mitochondrial-Permeability-Transition-Pore-Evaluated-by-Calcium-Retention-Capacity-Assay.aspx. (news-medical.net)
AIMS: To review the role played by the mitochondrial permeability transition pore (MPTP) in different physiological and pathological processes. (lu.se)
This being the case, the action would be mediated by the permeability of its membranes, through the formation of the mitochondrial permeability transition pore, and would involve phenomena such as the dissipation of the mitochondrial electrochemical potential and the release of substances from within it. (lu.se)

In MDA-MB-231 cells, ST caused a significant dose-dependent cell growth inhibition by 31- 63% (p ≤ 0.0001) in 48 h and 40-50% (p ≤ 0.0001) in 72 h. (biomedcentral.com)
We show for the first time that proadifen is able to enhance the cytotoxic properties of MTX in cBCRP cells, particularly through the inhibition of BCRP expression and activity. (spandidos-publications.com)
Moreover, we revealed the ability of proadifen to increase the intracellular hypericin content in HT-29 cells most likely via the inhibition of BCRP and MRP1 transporters ( 8 ). (spandidos-publications.com)
Guanidine is thought to act by increasing free intracellular calcium concentrations through inhibition of mitochondrial respiration. (medscape.com)

In multiple species, certain tissue types are prone to acquiring greater loads of mitochondrial genome (mtDNA) mutations relative to others, however the mechanisms that drive these heteroplasmy differences are unknown. (biorxiv.org)
The composition of the mitochondrial DNA (mtDNA) in each cell is in constant flux through processes of mutation, replication, and degradation. (biorxiv.org)
Alternatively, due to the high rate and relaxed replication of mtDNA, mosaicism can arise through clonal expansion and subsequent genetic drift between adjacent cells and tissues over time [ 6 ]. (biorxiv.org)
Indeed, there is continuous replication of mtDNA in all tissues, allowing variations in heteroplasmy to even develop between post-mitotic cells. (biorxiv.org)
3 For instance, mtDNA mutations and mitochondrial dysfunction have been observed in various types of cancer. (rsc.org)
Had they gone further, we believe they'd have found changes in mitochondrial DNA (mtDNA) as well. (earthpulse.net)

This observation prompted us to characterise the cell-autonomous cytotoxic and anti-proliferative profile of gomesin in DFTD cells and in comparison, to non-transformed (healthy) Tasmanian devil fibroblasts (FIBS). (nature.com)
The EasySep™ Human CD56 Positive Selection Kit II is designed to isolate CD56+ cells from fresh or previously frozen peripheral blood mononuclear cells and human skeletal muscle (myoblasts and fibroblasts) cultures by positive selection. (stemcell.com)
Results: LED produced in vitro increases of cell growth of 140-200% in mouse-derived fibroblasts, rat-derived osteoblasts, and rat-derived skeletal muscle cells, and increases in growth of 155-171% of normal human epithelial cells. (researchgate.net)
In vitro studies using infra-/red light have not shown any useful impact on wound healing by activating cells located in deeper skin layers such as fibroblasts or stem cells [2] . (researchgate.net)
CTAB has been shown as a promising compound against head and neck cancer cells with minimal toxicity against normal fibroblasts, as well as a tumoricidal irritant in colorectal cancer surgery. (rsc.org)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

JC-10™, a derivative of JC-1, is potential-dependent probe used to determine ΔΨm by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. (stratech.co.uk)
JC-10™ is compatible with fluorescence microplate readers, cell imagers and flow cytometers. (stratech.co.uk)
As membrane potential decreases, JC-1 becomes monomers, which shows in green fluorescence. (dojindo.com)
The change in ratio of red to green fluorescence is used as a indicator of mitochondrial condition. (dojindo.com)
Red fluorescence indicates normal membrane potential or health mitochondria. (dojindo.com)
Untreated cells showed red fluorescence, while FCCP treated cells showed little red fluorescence. (dojindo.com)
After opening of MPTP, fluorescence of membrane-impermeable CalciumGreen™ dye is enhanced as Ca 2+ leaches into the buffer. (news-medical.net)
Fluorescent indicator dyes (fluorophores) are most commonly used in biochemical and cell-based assays in vertebrate cell culture since fluorescence is more sensitive 3 and fluorescence is utilized in many instruments (microscopy, spectroscopy, flow cytometry). (nature.com)
In this study, we investigated the potential mechanism underlying the anti- C. albicans effects of AMP-17 using flow cytometry, transmission electron microscopy, fluorescent probes, fluorescence microplate reader, and confocal laser microscopy. (researchsquare.com)
The SCSA measures the shift from green (native, ds DNA) to red (denatured, ss DNA) fluorescence in each of 5000 cells per sample and the extent of this denaturation is quantified by alpha t [alpha t, = red/(red+green) fluorescence]. (purdue.edu)
After staining with R123, fluorescence intensity in the sperm midpiece is related to the mitochondrial membrane potential and motility. (purdue.edu)
Red fluorescence from the PI stained nuclear DNA results from compromised cell membranes and is indicative of dead or dying cells. (purdue.edu)
SYBRI4, a membrane permeable DNA stain (green fluorescence) requires an intact cell membrane for optimal fluorescence. (purdue.edu)
PI, a membrane impermeable stain (red fluorescence) stains dead or dying cells with breaks in the cell membranes. (purdue.edu)
Figure 4: Two parameter green (log) versus red (linear) fluorescence isometric displays of 10,000 cells collected from fertile (A) and less fertile (B) bull sperm stained with SYBRI4 and counterstained with Propidium Iodide (PI). (purdue.edu)

When the membrane potential is considered, PCFT generates substantial transmembrane electrochemical-potential gradients at extracellular pH levels relevant to the tumor microenvironment. (aspetjournals.org)

It plays a key role in mitochondrial homeostasis through selective elimination of dysfunctional mitochondria, and is an essential component of mitochondrial calcium homeostasis. (stratech.co.uk)
Mitochondrion is a common membrane-coated organelle that participates in energy conversion, homeostasis of calcium, cell signaling and cell death. (rsc.org)

Resveratrol seems to have a therapeutic gain by making tumor cells more sensitive to radiation and making normal tissue less sensitive. (scienceblog.com)
We injected 4T1 cells into 50 Balb/c mice to generate tumor xenografts. (bioone.org)
Furthermore, treatment of s.c xenografts derived from A549 cells with intratumor injections of terpinen-4-ol significantly inhibited tumor growth compared with the control group. (hindawi.com)
In addition to inhibiting pathogens such as bacteria and fungi, AMPs also have varying degrees of killing effects on viruses, tumor cells, and parasites (Falanga et al. (researchsquare.com)
Moreover, in vivo studies confirm that the CTAC@MSNs-HSA are able to induce cancer cell death and efficiently inhibit tumor growth. (rsc.org)
It has also been shown that metformin and phenformin inhibit tumor growth by disrupting mitochondrial complex. (rsc.org)

Is the Subject Area "Outer membrane proteins" applicable to this article? (plos.org)
A relevant comparison is that the presence of knife-racks, cutting boards and gloves in kitchen (analogous to enzymes like superoxide dismutase and catalase, membrane-embedded proteins with one-electron active redox centers, etc.) does not mean that knife is a dangerous component that must be avoided. (wikipedia.org)
Reduced the function of proteins in the pancreatic cancer cell membranes that are responsible for pumping chemotherapy out of the cell, making the cells chemo-sensitive. (scienceblog.com)
Multidrug resistance caused by the overexpression of ABC transporter proteins in cancer cells remains a major obstacle limiting chemotherapy efficacy. (spandidos-publications.com)
It was previously reported that proadifen (SKF-525A), a well-known cytochrome P450 monooxygenase inhibitor, not only has anti-proliferative potential in some cancer cell lines, but it is also able to inhibit BCRP and MRP1 transporter proteins ( 8 ). (spandidos-publications.com)
This result and other findings suggested that Mgr2p controls the lateral release of inner membrane proteins harboring a stop-transfer signal that follows an N-terminal amino acid signal. (frontiersin.org)
The biogenesis of most proteins in the mitochondrial matrix and the inner membrane relies on the function of the TIM23 complex. (frontiersin.org)
The main issue with trypan blue is its capacity to bind to proteins in the cell suspension, not just that released by dead cells. (nature.com)
Such dependant enzymes include those of the citric acid cycle, the mitochondrial electron transport chain, and several other pathways in the metabolism of carbohydrates, fats, and proteins. (medscape.com)

Mitochondrial condition in the carbonyl cyanide m-chlorophenyl hydrazine (CCCP) treated Parkin-expressing HeLa cells was compared with untreated cells using Mitophagy Detection Kit (MD01, MT02) and JC-1 MitoMP Detection Kit (MT09). (dojindo.com)
Mitophagy was not detected in untreated cells and the membrane potential was normal. (dojindo.com)
However, reduction of membrane potential and mitophagy were observed in treated cells. (dojindo.com)
We found that the conserved PTEN-induced putative kinase (PINK1/PINK-1) and the E3 ubiquitin-protein ligase parkin (PDR-1), which are required for mitochondrial autophagy (mitophagy), underlie stereotyped differences in heteroplasmy of a deleterious mitochondrial genome mutation (ΔmtDNA) between major somatic tissues types in Caenorhabditis elegans . (biorxiv.org)

[ 7 ] FAD is also a coenzyme needed for the functioning of the antioxidant enzyme glutathione reductase in its protection of cells against oxidative stresses, allowing for the measurement of the enzyme's activity in red blood cells to be among the methods for the assessment of riboflavin nutritional status. (medscape.com)

DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)

Several compounds have been shown to inhibit the efflux activity of ABC transporters, thereby increasing intracellular drug accumulation and sensitising cancer cells to therapy. (spandidos-publications.com)
The augmentation of intracellular pH, when cells are in a HEPES buffer, should be taken into consideration in studies that encompass all proton-coupled transporter families. (aspetjournals.org)

Melatonin was not toxic in NIH/3T3 primary mouse embryonic fibroblast cells at low doses for 24 and 48 h. (tubitak.gov.tr)

This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)
This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)

7,8 Chloroquine, an United States Food and Drug Administration-approved inhibitor of autophagy, has been revealed to possess antitumor property for impairing mitochondrial metabolism. (rsc.org)

We focus on developing assays that allow researchers to study cellular functions using cell-permeable reagents. (biosciregister.com)

In pSAE cells, cytotoxicity, enhancement in ROS, and dissipation of mitochondrial membrane potential caused by PC, PC-CNT, and PU-CNT were generally lower in magnitude compared to BEAS-2B cells at treatments examined, and is likely attributable to differences in depositional characteristics between the respective culture media for both respective cell lines. (cdc.gov)

Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)

Lowering of mitochondrial activity and dysfunction are known to be closely related to cancer, aging, and neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. (dojindo.com)

The mitochondrial membrane potential (ΔΨm), generated by the electron transport chain, is a key parameter necessary for healthy mitochondrial functioning. (stratech.co.uk)

Because mitoxantrone (MTX) is a strong BCRP substrate and is often used in the treatment of leukemia, we investigated the effect of 24 h proadifen pre-treatment on the cytotoxicity of MTX in leukemic cell lines that are sensitive to MTX (HL-60) and MTX-resistant ABCG2-overexpressing subclone (cBCRP). (spandidos-publications.com)
After exposure, both cell lines were assessed for cytotoxicity, ROS, and mitochondrial membrane potential. (cdc.gov)

Besides affecting fertilization and the outcome of pregnancy, sperm DNA damage has a negative impact on the health of the offspring as uncorrected sperm DNA damage following zygote formation has the potential to create mutations/epimutations in the offspring [ 7 ]. (hindawi.com)

A dose-dependent effect on mitochondrial membrane potential loss was observed. (ejbiotechnology.info)

The GSK3 Signaling Axis Regulates Adaptive Glutamine Metabolism in Lung Squamous Cell Carcinoma. (uclahealth.org)

Transmission electron microscopy showed that, following AMP-17 treatment, the shape of C. albicans cells became irregular, and vacuoles could be seen in the cytoplasm. (researchsquare.com)

This group includes lysosomal storage disorders, various mitochondrial diseases, other neurometabolic disorders, and several other miscellaneous disorders. (medscape.com)

Both dyes have been successfully for dymanic and in situ quantitative measurements, to screen for inhibitors of the mitochondrial transition pore, to assess the functionality of mitochondria in living cells, and can be used to discrimate between viable and non-viable cell populations. (stratech.co.uk)
Potentiometric dyes for measuring mitochondrial membrane potential. (stratech.co.uk)

This process allows in situ detection of cell or tissue specific events, while maintaining morphology and architecture. (biosciregister.com)

Camptothecin induced mitochondrial membrane potential changes were measured with JC-10™ (Cat No. 22204 ) and JC-1 (Cat No. 22200 ) in Jurkat cells. (stratech.co.uk)
After Jurkat cells were treated with camptothecin (10 µM) for 4 hours, JC-1 and JC-10™ dye loading solutions were added to the wells and incubated for 30 minutes. (stratech.co.uk)

The gating of this protein-selective pore has been extensively studied in patch clamp experiments on isolated mitochondrial membranes. (frontiersin.org)
9 Although the design of nanoparticles with triphenylphosphonium cation (TPP, the mitochondrion-targeting moiety) offers potential for mitochondrion-targeting therapeutics, it is still a challenge to actively deliver therapeutic agents to mitochondria due to the selective permeability of the mitochondrial membrane. (rsc.org)

Quite similarly, the cellular machinery has evolved to harness the reaction potential of DRS. The aesthetic perspective/concern that DRS would wreak havoc in routine physiology is no more relevant because several decades of research has now clearly established that DRS are routinely observed and unavoidable in physiology, and they cannot be just wished away. (wikipedia.org)
In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells. (hindawi.com)
It is important to systematically combine multiple endpoints, such as membrane integrity and cell death mechanism, to gain insight into the potential pathways involved in cellular toxicity. (nature.com)
Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)
Mitochondria play an important role in supplying cellular energy, cell signaling and governing cell death. (rsc.org)
To study the nonthermal effects of radiofrequency fields on human immune cells ( peripheral blood mononuclear cells ) exposed to a Global System for Mobile Communication ( GSM ) signal generated by a commercial cellular phone and by a sinusoidal non- modulated signal ( continuous wave ). (emf-portal.org)

The TIM23 complex is a hub for translocation of preproteins into or across the mitochondrial inner membrane. (frontiersin.org)
Deletion of Mgr2p has been found to delay protein translocation into the matrix and accumulation in the inner membrane. (frontiersin.org)
The core of the TIM23 complex contains a pore that is essential for protein insertion into or translocation across the inner membrane. (frontiersin.org)

Cell-permeable cationic rhodamines, such TMRE and TMRM, are readily sequestered by active mitochondria, and commonly used to label mitochondria in living cells. (stratech.co.uk)

The further development of scleractinian coral cell cultures requires quantitative assessment of survivorship through basic live and dead cell counts. (nature.com)

TMRE (Tetramethylrhodamine ethyl ester, perchlorate) is used to quantify changes in mitochondrial membrane potential in live cells. (biotium.com)

Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. (hindawi.com)
FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)
Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)

Hence, for the prevention of cell death in a variety of disease conditions, the MPTP is a potential target. (news-medical.net)

The use of trypan blue, a colorimetric, cell impermeant stain, to quantify cell death is the most reported method in relation to coral cell cultures despite its limitations. (nature.com)

It inhibits respiration by blocking potassium channels and thus prolonging the nerve terminal action potential. (medscape.com)

The percentages of early/late apoptotic cells to which melatonin (IC50: 380 μM) was administered were increased 6.2-fold in 5RP7 cells compared to controls for 48 h. (tubitak.gov.tr)
CNPs increased the proportion of apoptotic cells, down-regulated Bcl-2 expression and up-regulated Bax expression. (nih.gov)

These findings would form the basis for further studies to understand the mechanisms and assess the potential utility of ST as a cancer chemopreventive agent against breast cancer. (biomedcentral.com)
We sought to identify PKA phosphorylation sites in PLIN5 and assess their functional relevance in cultured cells and the livers of mice. (uci.edu)

Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)
High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)

Since the bead concentration is known, a simple calculation determines the sperm concentration, an important parameter of fertility potential. (purdue.edu)
Co-staining sperm with SYBRI4 and PI readily identifies cells with intact membranes versus membrane compromised cells. (purdue.edu)

ST caused G0/G1 cell cycle arrest which was accompanied by a decrease in CDK4 and cyclin D1, and an increase in p21/Cip1and p27/Kip1 protein levels. (biomedcentral.com)
Depolarized the mitochondrial membranes, which indicates a decrease in the cell's potential to function. (scienceblog.com)
But just as reseveratrol interferes with the cancer cells' energy source, it also may decrease the power available to pump chemotherapy out of the cell. (scienceblog.com)

In conclusion, the evidence supports gomesin as a potential antiproliferative compound against DFTD disease. (nature.com)
This type of compound screening is crucial in the early stages of drug development in order to determine the potential toxic effects of the drug. (enzolifesciences.com)

P. damicornis cells exposed for 24 h to Triton-X100, insulin or titanium dioxide (TiO 2 ) NPs, respectively, at concentrations ranging from 0.5 to 100 µg/mL, revealed a LC50 of 0.46 µg/mL for Triton-X100, 6.21 µg/mL for TiO 2 NPs and 33.9 µg/mL for insulin. (nature.com)

Anatomy47

Organisms16

Diseases1

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes60

Disciplines and Occupations4

Technology, Industry, Agriculture6

Information Science3

Health Care1

Loss of mitochondrial membrane potential4

Apoptosis23

Reactive oxygen4

Proliferation4

Viability9

Necrosis1

Depolarization2

Permeability tran4

Inhibition4

MtDNA6

Fibroblasts6

Fluorescence15

Transmembrane1

Calcium2

Tumor6

Proteins9

Mitophagy4

Measurement1

Ligands1

Intracellular2

Mouse embryonic1

Redox2

Autophagy1

Reagents1

Dissipation1

Induce1

Dysfunction1

Electron trans1

Cytotoxicity2

Mutations1

Dose-dependent1

Metabolism1

Microscopy1

Lysosomal1

Dyes2

Morphology1

Jurkat2

Selective2

Cellular6

Translocation3

Permeable1

Quantitative1

Live cells1

Involvement3

MPTP1

Quantify1

Inhibits1

Apoptotic cells2

Assess2

Metabolic2

Sperm2

Decrease3

Compound2

Concentrations1