• methylation
  • Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm Matthew Rea 1 , Ming Chen 1 , Shan Luan 1 , Drutdaman Bhangu 1 , Max Braud 1 , Wenyan Xiao 1 1 Department of Biology, Saint Louis University Imprinting is a phenomenon in plant and mammal reproduction. (jove.com)
  • Isolating endosperm and determining methylation status of imprinted genes in Arabidopsis can be difficult. (jove.com)
  • repetitive
  • The residual fifth is moderately to highly repetitive, and can be divided into two types, depending on whether the individual repeat units are dispersed singularly (interspersed repetitive DNA) or clustered together (satellite DNA). (bmj.com)
  • There are several families of interspersed repetitive DNA, although the two largest are known as short and long interspersed nuclear elements (SINEs and LINEs, respectively), 1 and it is to the SINE family that the frequently mentioned Alu repeat belongs. (bmj.com)
  • telomerase
  • We previously showed that, in primates, ITSs were inserted during the repair of DNA double-strand breaks and proposed that they could arise from either the capture of telomeric fragments or the action of telomerase. (biomedcentral.com)
  • slippage
  • Interestingly, 5% of the single base pair substitutions might represent double-slippage events that occurred at the junction of immediately adjacent repeats, resulting in a shift in the repeat boundary. (g3journal.org)
  • diseases
  • Although the presence of these subtle mechanisms may be beneficial to a whole population, when they go wrong, as is thought to happen in the case of human trinucleotide repeat associated diseases, such as Huntington's disease, the consequences for the individual can be fatal. (bmj.com)
  • deletions
  • The telomeric repeat insertion occurred either without modification of the sequence at the break site or with processing of the ends produced by the break involving deletions, insertions or target site duplications [ 17 ] (Additional data file 1). (biomedcentral.com)
  • disorders
  • however, they are extremely useful in such fields as forensic DNA profiling and genetic linkage analysis, which can be used to search for genes involved in a wide range of disorders. (bmj.com)
  • Some of these strategies have the potential for future use in gene therapy for trinucleotide repeat disorders. (lidsen.com)
  • humans
  • Nevertheless, even focusing as intended upon microsatellites and their relevance to humans, it should become clear that most of these questions remain ones that will require consideration, even if only in passing. (bmj.com)
  • human
  • However, when used to refer to the number of repeating units of genetic information that are sufficient to encode the blueprint for something as complex as a human being, it never ceases to amaze me that this number is big enough! (bmj.com)
  • genetic
  • Although the transfer of a number of genes from these organelles to the nucleus prevents them from living independently, each still possesses genetic material in the form of double stranded DNA. (wikipedia.org)
  • distinct
  • Although in prokaryotes distinct biological functions have been demonstrated, the role of microsatellites in eukaryotes is less clear. (bmj.com)
  • leads
  • The increase in the number of repeats over time leads to earlier development and increased severity of symptoms in affected individuals in successive generations. (lidsen.com)
  • levels
  • In breast cancer, a dinucleotide CA-repeat within the first intron of the epidermal growth factor receptor ( EGFR ) gene correlates with the gene's transcription levels. (biomedcentral.com)