• Hepatic Microsomes
  • To verify the direct inhibitive effect of CO on CYP2E1 activity, rat hepatic microsomes were isolated by ultracentrifuge and incubated with ruthenium-containing CO releasing molecular (tricarbonyldichlororuthenium dimmer, CORM-2) which has been confirmed to possess the effects similar to CO administration as a gas. (thefreedictionary.com)
  • Aggregates were formed when clear supernatants from hepatic microsomes that had been treated with steapsin were desalted and concentrated. (sciencemag.org)
  • Garry Duthie and Philip Morrice, "Antioxidant Capacity of Flavonoids in Hepatic Microsomes Is not Reflected by Antioxidant Effects In Vivo ," Oxidative Medicine and Cellular Longevity , vol. 2012, Article ID 165127, 6 pages, 2012. (hindawi.com)
  • In Smith-Lemli-Opitz homozygotes, the transformation of 7-dehydrocholesterol to cholesterol by hepatic microsomes was blocked although 7-dehydrocholesterol was produced abundantly from lathosterol. (jci.org)
  • This was subsequently used to compare propofol hydroxylase activities in hepatic microsomes obtained from greyhound, beagle, and mixed-breed dogs. (aspetjournals.org)
  • microsomal
  • Final pellets (Sf21 microsomes) were resuspended at approximately 5 mg/ml in 250 mM sucrose, 30 mM histidine (pH 7.4) and stored in small aliquots at -40 °C. The average yield per 600 ml of infection was 40 mg of microsomal protein. (bio.net)
  • MeSH A11.284.835.540 The use of microsomes to measure clearance The performance of in vitro microsomal clearance studies including analysis of the data. (wikipedia.org)
  • In contrast to our previous report showing lower level of BaP phenols versus BaP diols/diones in the whole-cell, this report demonstrated that the sum of microsomal BaP phenolic metabolites were ~60% greater than that of the BaP diols/diones after exposure of microsomes to BaP. (sigmaaldrich.com)
  • metabolites
  • Taken together, upregulation of phase II XMEs and CYP1 proteins, but not EH1 in the ER might be the mechanism by which overexpression of catalase reduces the levels of all the BaP metabolites, and enhances the ratio of BaP phenolic metabolites versus diol/diones in endothelial microsomes. (sigmaaldrich.com)
  • Analysis of metabolites produced by tracheal epithelial microsomes with high-pressure liquid chromatography showed that diols, quinones, and phenols of BP were the major metabolic products formed during 30-min incubations. (aacrjournals.org)
  • Tissues
  • The catalytic efficiencies of microsomes from known susceptible tissues/subcompartments are 10 and 250 fold higher than in rat airway and monkey alveolar subcompartments, respectively. (osti.gov)
  • insect
  • The insect cell microsomes are clumpy when isolated either in solutions containing high salt (0.6 M KCl, 250 mM sucrose, 3 mM MgCl2) or low salt (10 mM NaHCO3, 0.1 mM CaCl2). (bio.net)
  • Are there solutes to add (pyrophosphate, NaI, etc) that would allow easy and complete resuspension of insect cell microsomes? (bio.net)
  • Cells
  • microsomes are not present in healthy, living cells. (wikipedia.org)
  • To examine the involvement of ER in catalase-induced BaP detoxification, we compared the level and distribution of XMEs, and the profile of BaP intermediates in the microsomes of wild-type and catalase transgenic endothelial cells. (sigmaaldrich.com)