• liver
  • The conversion of cyclophosphamide to its alkylating metabolite(s) by microsomes obtained from male rat liver was characterized. (aacrjournals.org)
  • Markedly inhibited 7-dehydrocholesterol-delta 7-reductase activity in liver microsomes from Smith-Lemli-Opitz homozygotes. (jci.org)
  • Using isotope incorporation assays, we measured the transformation of the precursors, [3 alpha- 3H]lathosterol and [1,2-3H]7-dehydrocholesterol into cholesterol by liver microsomes from seven controls and four Smith-Lemli-Opitz homozygous subjects. (jci.org)
  • The introduction of the double bond in lathosterol at C-5 to form 7-dehydrocholesterol that is catalyzed by lathosterol-5-dehydrogenase was equally rapid in controls and homozygotes liver microsomes (120 +/- 8 vs 100 +/- 7 pmol/mg protein per min, P = NS). (jci.org)
  • Thus, lathosterol 5-dehydrogenase is equally active which indicates that homozygotes liver microsomes are viable. (jci.org)
  • Incorporation of 14 C into the phospholipid components of the liver microsomes isolated by a conventional method was determined quantitatively using β-scintillation measurement after preceding TLC separation. (springer.com)
  • This effect correlated strongly with an inhibition of MFO as shown by a decrease in the O-demethylation of p-nitroanisol in prepared liver microsomes. (springer.com)
  • An in vitro propofol hydroxylase assay was developed and then used to compare enzyme activities in liver microsomes from male greyhound, beagle, and mixed-breed dogs (five each). (aspetjournals.org)
  • Hyperimmune spleen DNA was hybridized with radiolabeled RNA from spleen and myeloma microsomes in the presence of unlabeled liver RNA. (jimmunol.org)
  • CYP2C9 is involved in the formation of hydroxygliclazide in human liver microsomes and in a panel of recombinant human P450s in vitro. (wikipedia.org)
  • Retinol esterification by rat liver microsomes. (wikipedia.org)
  • hepatic
  • Aggregates were formed when clear supernatants from hepatic microsomes that had been treated with steapsin were desalted and concentrated. (sciencemag.org)
  • In Smith-Lemli-Opitz homozygotes, the transformation of 7-dehydrocholesterol to cholesterol by hepatic microsomes was blocked although 7-dehydrocholesterol was produced abundantly from lathosterol. (jci.org)
  • This was subsequently used to compare propofol hydroxylase activities in hepatic microsomes obtained from greyhound, beagle, and mixed-breed dogs. (aspetjournals.org)
  • microsomal
  • Final pellets (Sf21 microsomes) were resuspended at approximately 5 mg/ml in 250 mM sucrose, 30 mM histidine (pH 7.4) and stored in small aliquots at -40 °C. The average yield per 600 ml of infection was 40 mg of microsomal protein. (bio.net)
  • In contrast to our previous report showing lower level of BaP phenols versus BaP diols/diones in the whole-cell, this report demonstrated that the sum of microsomal BaP phenolic metabolites were ~60% greater than that of the BaP diols/diones after exposure of microsomes to BaP. (sigmaaldrich.com)
  • MeSH A11.284.835.540 The use of microsomes to measure clearance The performance of in vitro microsomal clearance studies including analysis of the data. (wikipedia.org)
  • nmol
  • Estimates of apparent K m and V max for propofol hydroxylation by microsomes from a male beagle dog were 7.3 μM and 3.8 nmol/mg/min, respectively. (aspetjournals.org)
  • At a substrate concentration of 20 μM, propofol hydroxylase activity was significantly lower ( p = .032) in greyhound microsomes (1.7 ± 0.4 nmol/mg/min) compared with beagle microsomes (5.1 ± 1.3 nmol/mg/min) but was not statistically different ( p = .42) compared with mixed-breed microsomes (3.1 ± 1.2 nmol/mg/min). (aspetjournals.org)
  • mice
  • Microsomes from livers of female rats and mice metabolized cyclophosphamide with a V max of 0.66 and 9.69 µmoles/g/hr, respectively, and a K m of 0.67 and 0.68 m m , respectively. (aacrjournals.org)
  • Microsomes prepared from the mouse myeloma MOPC 104E and from spleens of mice hyperimmunized with Salmonella typhi were used as a source of RNA rich in immunoglobulin mRNA. (jimmunol.org)
  • Tissues
  • The catalytic efficiencies of microsomes from known susceptible tissues/subcompartments are 10 and 250 fold higher than in rat airway and monkey alveolar subcompartments, respectively. (osti.gov)
  • metabolites
  • Taken together, upregulation of phase II XMEs and CYP1 proteins, but not EH1 in the ER might be the mechanism by which overexpression of catalase reduces the levels of all the BaP metabolites, and enhances the ratio of BaP phenolic metabolites versus diol/diones in endothelial microsomes. (sigmaaldrich.com)
  • Cells
  • To examine the involvement of ER in catalase-induced BaP detoxification, we compared the level and distribution of XMEs, and the profile of BaP intermediates in the microsomes of wild-type and catalase transgenic endothelial cells. (sigmaaldrich.com)
  • microsomes are not present in healthy, living cells. (wikipedia.org)
  • cell
  • Low salt protocol: For preparation of Sf21 cell microsomes, the washed cell pellets were resuspended in 25 ml of medium containing 10 mM NaHCO3 and 0.2 mM CaCl2, supplemented with the protease inhibitors aprotinin (1 µg/ml), leupeptin (0.2 µg/ml), pepstatin A (1 µg/ml), and Pefabloc SC (0.1 mM). (bio.net)