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  • NSOM
  • Previously, IR-NSOM was realized by applying a solid immersion lens with a refractive index of n, which shortens wavelength (λ) to (λ/n), compared to FTIR-based IR microscopy. (wikipedia.org)
  • atomic
  • A photoionization microscope is an instrument that uses photoionization, along with quantum properties and principles, in order to measure atomic properties.The principle behind photoionization microscopy is to study the spatial distribution of electrons ejected from an atom in a situation in which the De Broglie wavelength becomes large enough to be observed on a macroscopic scale. (wikipedia.org)
  • Chemical force microscopy (CFM) is a variation of atomic force microscopy (AFM) which has become a versatile tool for characterization of materials surfaces. (wikipedia.org)
  • microscopes
  • Fluorescence illumination and observation is the most rapidly expanding microscopy technique employed today, both in the medical and biological sciences, a fact which has spurred the development of more sophisticated microscopes and numerous fluorescence accessories. (fsu.edu)
  • Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). (wikipedia.org)
  • interference
  • Interference microscopy involving measurements of differences in the path between two beams of light that have been split. (wikipedia.org)
  • With this design (marketed by E. Leitz) 60 mm beam separation was achieved in microscopy but here the new difficulty has arisen of balancing optical thicknesses of two separate microscope slide preparations (sample and dummy) and maintaining this critical balance during longer observations (e.g. time-lapse studies of living cells maintained at 37 °C), otherwise a gradual change in background interference colour occurs over time. (wikipedia.org)
  • The main advantage offered by interference microscopy measurements is the possibility of measuring the projected dry mass of living cells, which was first effectively exploited by Andrew Huxley in studies of striated muscle cell structure and function, leading to the sliding filament model of muscle contraction. (wikipedia.org)
  • Interference microscopy became relatively popular in the 1940-1970 decades but fell into disuse because of the complexity of the instrument and difficulties in both its use and in the interpretation of image data. (wikipedia.org)
  • Interference microscopy for industrial inspection, semiconductor inspection and surface structure analysis is highly developed and in widespread use. (wikipedia.org)
  • microscopic
  • Quantum microscopy is a novel tool that allows microscopic properties of matter and quantum particles to be measured and directly visualized. (wikipedia.org)
  • Nanovid microscopy, from "nanometer video-enhanced microscopy", is a microscopic technique aimed at visualizing colloidal gold particles of 20-40 nm diameter (nanogold, immunogold) as dynamic markers at the light-microscopic level. (wikipedia.org)
  • optical
  • The field of microscopy (optical microscopy) has its roots in the 17th-century Dutch Republic, with some notable representatives such as Zacharias Janssen, Hans Lippershey, Jan Swammerdam, and Antonie van Leeuwenhoek. (wikipedia.org)
  • Optical or light microscopy involves passing visible light transmitted through or reflected from the sample through a single lens or multiple lenses to allow a magnified view of the sample. (wikipedia.org)
  • Interferometric microscopy allows enhancement of resolution of optical microscopy due to interferometric (holographic) registration of several partial images (amplitude and phase) and the numerical combining. (wikipedia.org)
  • It is the second concept to overcome the diffraction barrier in far-field optical microscopy published by Stefan Hell. (wikipedia.org)
  • Since ultrasonic scattering in tissue is weaker than optical scattering, photoacoustic microscopy is capable of achieving high-resolution images at greater depths than conventional microscopy methods. (wikipedia.org)
  • phase
  • In interferometric microscopy, the image of a micro-object is synthesized numerically as a coherent combination of partial images with registered amplitude and phase. (wikipedia.org)
  • As the combined image keeps both amplitude and phase information, the interferometric microscopy can be especially efficient for the phase objects, allowing detection of light variations of index of refraction, which cause the phase shift or the light passing through for a small fraction of a radian. (wikipedia.org)
  • particle
  • Microscopy is often used as an absolute method of particle size analysis since it is the only method in which the individual particles are observed and measured. (springer.com)
  • Kusumi A, Sako Y, Yamamoto M., Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). (wikipedia.org)
  • occurs
  • Photoacoustic microscopy takes advantage of the local temperature rise that occurs as a result of light absorption in tissue. (wikipedia.org)
  • technique
  • The development of microscopy revolutionized biology, gave rise to the field of histology and so remains an essential technique in the life and physical sciences. (wikipedia.org)
  • Intravital microscopy is a technique used to observe biological systems in vivo at high resolution through an attached window preparation. (wikipedia.org)
  • detector
  • In photoionization microscopy experiments, an atom in an electric field is ionized by a laser with sharply defined frequency, the electron is drawn toward a position-sensitive detector, and the current is measured as a function of position. (wikipedia.org)
  • contrast
  • Current research in photoacoustic microscopy takes advantage of both endogenous and exogenous contrast agents to gain functional information about the body, from blood saturation levels to cancer proliferation rate. (wikipedia.org)
  • Endogenous contrast agents, molecules naturally occurring within the body, are useful in photoacoustic microscopy due to the fact that they may be imaged non-invasively. (wikipedia.org)
  • light
  • Visible light photoacoustic microscopy is particularly useful in determining hemoglobin concentration and oxygen saturation due to the difference in absorption profiles of oxyhemoglobin and deoxyhemoglobin. (wikipedia.org)
  • In traditional microscopy, the resolution that can be obtained is limited by the diffraction of light. (wikipedia.org)
  • probe
  • however, as other kinds of the probe microscopy, SCM requires careful preparation of the analyzed surface, which is supposed to be almost flat. (wikipedia.org)
  • detection
  • In addition, photoacoustic microscopy is capable of early melanoma detection due to the high concentration of melanin found in skin cancer cells. (wikipedia.org)
  • molecules
  • Due to the fact that almost all molecules are capable of nonradiative relaxation, photoacoustic microscopy has the potential to image a wide range of endogenous and exogenous agents. (wikipedia.org)
  • images
  • It was not until 1996 that anything resembling the microscopy images proposed by Demakov and colleagues came to fruition. (wikipedia.org)
  • Virtual microscopy is a method of posting microscope images on, and transmitting them over, computer networks. (wikipedia.org)
  • method
  • Geerts H, De Brabander M, Nuydens R, Geuens S, Moeremans M, De Mey J, Hollenbeck P., Nanovid tracking: a new automatic method for the study of mobility in living cells based on colloidal gold and video microscopy, Biophys J. 1987 Nov;52(5):775-82. (wikipedia.org)
  • The capacitance microscopy method developed by Williams et. (wikipedia.org)
  • observation
  • As such, photoionization microscopy relies on the existence of interferences between various trajectories by which the electron moves from the atom to the plane of observation, for example, photoionization microscopy of the hydrogen atom in parallel electric and magnetic fields. (wikipedia.org)
  • tool
  • Expansion Microscopy (ExM) is a sample preparation tool for biological samples that allows investigators to identify small structures by expanding them using a polymer system. (wikipedia.org)
  • image
  • Virtual Microscopy Slide Database Whole slide image analysis blog by Flagship Biosciences Free Virtual Microscope Web Application with automated image analysis Digital Pathology Blog Virtual Microscopy of the Brain Virtual Microscopy a Disruptive Technology? (wikipedia.org)
  • One of the major benefits of photoacoustic microscopy is the simplicity of image reconstruction. (wikipedia.org)
  • surface
  • de Brabander, M. Nuydens, R. Ishihara, A. Holifield, B. Jacobson, K. and Geerts, H., Lateral diffusion and retrograde movements of individual cell surface components on single motile cells observed with Nanovid microscopy, J. Cell Biol. (wikipedia.org)