• specimen
  • Fluorescence emission gathered from the specimen is filtered through a confocal pinhole aperture to reject light that originates from regions removed from the focal plane. (zeiss.com)
  • biology
  • Refinements in design have simplified confocal microscopy to the extent that it has become a standard research tool in cell biology. (microscopyu.com)
  • The development of microscopy revolutionized biology, gave rise to the field of histology and so remains an essential technique in the life and physical sciences. (wikipedia.org)
  • image
  • The purpose of using coefficients is to characterize the degree of overlap between images, usually two channels in a multidimensional microscopy image recorded at different emission wavelengths. (wikipedia.org)
  • subcellular
  • Today, more and more researchers are interested in linking macroscopic properties and behavior - such as memory learning - with microscopy at the single cell and subcellular level, as well as studying molecular events such as gene control and neuronal activity like excitatory and inhibitory signaling. (photonics.com)
  • Methods
  • It often involves a biopsy for subsequent laboratory analysis with various methods that include visual microscopy . (photonics.com)
  • Up to now, most frequently used methods to quantify colocalization calculate the statistical correlation of pixel intensities in two distinct microscopy channels. (wikipedia.org)
  • images
  • Linear lobe deconvolution as well as nonlinear restoration of 4Pi confocal raw data with their point-spread functions (PSF's) leads to almost-identical images, irrespective of whether the 4Pi confocal PSF relies on constructive or destructive interference. (osapublishing.org)
  • Good optical sectioning, often referred to as good depth or z resolution, is popular in modern microscopy as it allows the three-dimensional reconstruction of a sample from images captured at different focal planes. (wikipedia.org)