• optical
  • Confocal microscopy provides the capacity for direct, noninvasive, serial optical sectioning of intact, thick, living specimens with a minimum of sample preparation as well as a marginal improvement in lateral resolution. (wikipedia.org)
  • This interactive tutorial explores optical sectioning with confocal microscopy and compares these sections to the results obtained with widefield fluorescence. (zeiss.com)
  • For an optical cavity consisting of two mirrors, confocal means that they share their foci. (wikipedia.org)
  • Many different techniques for optical sectioning are used and several microscopy techniques are specifically designed to improve the quality of optical sectioning. (wikipedia.org)
  • Good optical sectioning, often referred to as good depth or z resolution, is popular in modern microscopy as it allows the three-dimensional reconstruction of a sample from images captured at different focal planes. (wikipedia.org)
  • In comparison, the lateral resolution can be approximated as: D x = D y = 0.61 λ N A {\displaystyle D_{x}=D_{y}={\frac {0.61\lambda }{\mathrm {NA} }}} Beyond increasing numerical aperture, there are few techniques available to improve optical sectioning in bright-field light microscopy. (wikipedia.org)
  • Confocal microscopy, another optical technique, typically penetrates less deeply into the sample but with higher resolution. (wikipedia.org)
  • The field of microscopy (optical microscopy) has its roots in the 17th-century Dutch Republic, with some notable representatives such as Zacharias Janssen, Hans Lippershey, Jan Swammerdam, and Antonie van Leeuwenhoek. (wikipedia.org)
  • Optical or light microscopy involves passing visible light transmitted through or reflected from the sample through a single lens or multiple lenses to allow a magnified view of the sample. (wikipedia.org)
  • situ
  • Researchers and clinicians have recognized that in situ microscopy, in conjunction with endoscopic procedures, would help diagnose disease earlier. (photonics.com)
  • vivo
  • Confocal microscopy provides high-resolution, high-contrast in vivo images and is a powerful tool for studying the ultrastructure of the cell, its molecular components, and their functions. (hindawi.com)
  • Moreover
  • Moreover, restoration of 4Pi confocal images of the same object with alternate phases is introduced as a powerful test for (nonlinear) image restoration. (osapublishing.org)
  • biology
  • Refinements in design have simplified confocal microscopy to the extent that it has become a standard research tool in cell biology. (microscopyu.com)
  • The development of microscopy revolutionized biology, gave rise to the field of histology and so remains an essential technique in the life and physical sciences. (wikipedia.org)
  • image
  • The purpose of using coefficients is to characterize the degree of overlap between images, usually two channels in a multidimensional microscopy image recorded at different emission wavelengths. (wikipedia.org)
  • subcellular
  • Today, more and more researchers are interested in linking macroscopic properties and behavior - such as memory learning - with microscopy at the single cell and subcellular level, as well as studying molecular events such as gene control and neuronal activity like excitatory and inhibitory signaling. (photonics.com)
  • light
  • 5. The confocal interferometry system of claim 4 , wherein the first imaging system further comprises a refracting surface positioned between the object and the beam splitter to receive light rays from the object. (google.com.au)
  • Confocal microscopy uses a scanning point or points of light to illuminate the sample. (wikipedia.org)
  • Imaging
  • During more than a decade of biological confocal microscopy, the feature that has generally received the most emphasis is its capacity to achieve improved axial resolution when imaging fluorescently labeled preparations. (springer.com)
  • The requirements for accurate color imaging have been further increased by the development of colorimetric methods of quantitative microscopy, such as fluorescence ratio measurements of ion concentrations. (microscopyu.com)
  • The enhanced swept-field confocal scanning technology of the standard Opterra II allows for high-speed imaging at hundreds of frames per second. (bruker.com)
  • 2. The confocal interferometry system of claim 1 further comprising a second imaging system that images the array of pinholes onto the array of detector elements. (google.com.au)
  • images
  • Linear lobe deconvolution as well as nonlinear restoration of 4Pi confocal raw data with their point-spread functions (PSF's) leads to almost-identical images, irrespective of whether the 4Pi confocal PSF relies on constructive or destructive interference. (osapublishing.org)
  • point
  • 4. The confocal interferometry system of claim 3 wherein the reflecting surface is substantially concentric with a point on the object. (google.com.au)
  • Methods
  • It often involves a biopsy for subsequent laboratory analysis with various methods that include visual microscopy . (photonics.com)
  • Up to now, most frequently used methods to quantify colocalization calculate the statistical correlation of pixel intensities in two distinct microscopy channels. (wikipedia.org)