• specimens
  • We apply methods for determining the CTF parameters in low signal to noise images of tilted specimens, for monitoring defocus changes using observed magnification changes, and for correcting the CTF prior to reconstruction. (nih.gov)
  • The approval was based on a multi-center study of 1,992 cases in which whole-slide imaging was shown to be non-inferior to microscopy across a wide range of surgical pathology specimens, sample types and stains But unlike digital radiology where the elimination of film made return on investment (ROI) clear, the ROI on digital pathology equipment is less obvious. (wikipedia.org)
  • In pathology the scanning is done from preserved and processed specimens, for retrospective studies even from slides stored in a biobank. (wikipedia.org)
  • quantitative
  • In comparison to the strict mechanical and enzymatic DNA extraction methods, this novel hybrid method provided the best overall combined performance when considering quantitative (using 16S rRNA qPCR) and qualitative (using microbiomics) estimates of the total bacterial communities when processing poultry feces and litter samples. (jove.com)
  • spatial
  • This is in part because of its excellent spatial resolution, which allows researchers to identify brain areas associated with specific cognitive processes. (jove.com)
  • This is achieved by using a unique image-based computer modeling system, Virtual Cell, to integrate experimental data on the rates and spatial distributions of the key molecular components of the process. (nih.gov)
  • The spatial variation in this information (the "image") may be viewed by projecting the magnified electron image onto a fluorescent viewing screen coated with a phosphor or scintillator material such as zinc sulfide. (wikipedia.org)
  • measurements
  • Even when some sort of qualitative tools are available, such as a measuring reticle in the microscope, it has still required a human to determine and record those measurements. (wikipedia.org)
  • Beginning in the late 1800s with the availability of photographic plates, it became possible to capture microscope images permanently on film or paper, making measurements easier to acquire by simply using a scaled ruler on the hard copy image. (wikipedia.org)
  • While this significantly speeded up the acquisition of particle measurements, it was still a tedious, labor-intensive process, which not only made it difficult to measure statistically significant particle populations, but also still introduced some degree of human error to the process. (wikipedia.org)
  • As faster computing resources became available at lowered costs, the task of making measurements from microscope images of particles could now be performed automatically by machine without human intervention, making it possible to measure significantly larger numbers of particles in much less time. (wikipedia.org)
  • These two images can be a nuisance when they overlap, since they can severely affect the accuracy of mass thickness measurements. (wikipedia.org)
  • detection
  • The CTF is not routinely corrected in cryo-electron tomography because of difficulties including CTF detection, due to the low signal to noise ratio, and CTF correction, since images are characterised by a spatially variant CTF. (nih.gov)
  • acquisition
  • Static image acquisition is the most common form. (wikipedia.org)
  • In static image acquisition only one field of view image is captured at a time. (wikipedia.org)
  • In an effort to foster increase in its use as a research imaging method, and to ensure proper and accurate data collection, the present article will outline - in the framework of a paradigm using semantic categorization to examine the effects of antipsychotics and schizotypy on the N400 - the procedure and key aspects associated with ERP data acquisition. (jove.com)
  • maturation
  • Based on this evidence, we have proposed that PC-I is transported across the Golgi stacks by the cisternal maturation process. (nih.gov)
  • system
  • The first computer-assisted neuron reconstruction system, now known as Neurolucida, was developed by Dr. Edmund Glaser and Dr. Hendrik Van der Loos in the 1960s. (wikipedia.org)
  • The outputs of this system are the labels and the statistical data of all adipose cells in the image. (frontiersin.org)
  • Neuroscientists are developing new imaging techniques and generating large volumes of data in an effort to understand the complex structure of the nervous system. (utah.edu)
  • practice
  • Digital pathology is enabled in part by virtual microscopy, which is the practice of converting glass slides into digital slides that can be viewed, managed, shared and analyzed on a computer monitor. (wikipedia.org)
  • reconstruction
  • Neuron tracing, or Neuron reconstruction is a technique used in neuroscience to determine the path of neural axons and dendrites (many times also called neuronal processes). (wikipedia.org)
  • There are normally two processing steps: generation and proof editing of a reconstruction. (wikipedia.org)
  • Crowdsourcing is an alternative way to effectively collect collaborative manual reconstruction results for such image data sets. (wikipedia.org)
  • data
  • Transformation into a stereotaxic standard space is a prerequisite for group studies and requires thorough data processing to preserve directional inter-dependencies. (jove.com)
  • fundamental
  • The field of Radiology has undergone the digital transformation almost 15 years ago, not because radiology is more advanced, but there are fundamental differences between digital images in radiology and digital pathology: The image source in radiology is the (alive) patient, and today in most cases the image is even primarily captured in digital format. (wikipedia.org)
  • digital
  • Finally, beginning roughly in the late 1970s, CCD digital sensors for capturing images and computers which could process those images, began to revolutionize the process by using digital imaging. (wikipedia.org)
  • This type of set-up is often referred to as a digital microscope, although many systems using that name are used only for displaying an image on a monitor. (wikipedia.org)
  • Modern electron microscopes produce electron micrographs using specialized digital cameras and frame grabbers to capture the image. (wikipedia.org)
  • The image detected by the digital camera may be displayed on a monitor or computer. (wikipedia.org)
  • Digital pathology is an image-based information environment which is enabled by computer technology that allows for the management of information generated from a digital slide. (wikipedia.org)
  • With the advent of Whole-Slide Imaging, the field of digital pathology has exploded and is currently regarded as one of the most promising avenues of diagnostic medicine in order to achieve even better, faster and cheaper diagnosis, prognosis and prediction of cancer and other important diseases. (wikipedia.org)
  • Digital slides are accessible for viewing via a computer monitor and viewing software either locally or remotely via the Internet. (wikipedia.org)
  • sample
  • The sample is prepared on a microscope slide which is placed on the microscope stage. (wikipedia.org)
  • Once the sample has been focused on, then an image can be acquired and displayed on the monitor. (wikipedia.org)
  • If the user wishes to image other portions of the same sample on the slide, they can use the X-Y positioning hardware (typically composed of two linear stages on the microscope to move to a different area of the slide. (wikipedia.org)
  • Once the enzymatic extraction portion of the hybrid method was initiated, the remaining purification process was automated using a robotic workstation to increase sample throughput and decrease sample processing error. (jove.com)
  • contrast
  • The resolution of this technique is limited by the contrast transfer function (CTF) of the microscope. (nih.gov)
  • Non-invasive magnetic resonance imaging (MRI) can provide three-dimensional images of the infant brain in less than 20 minutes, with unprecedented anatomical details and contrast of brain anatomy, cortical and subcortical structures and brain connectivity. (utah.edu)
  • volumes
  • This allows the user to mark (or segment) structures and regions of interest in 3D image volumes using automatic, semi-automatic, and manual tools. (wikipedia.org)
  • overlap
  • Care must be taken to insure that two images do not overlap so as not to count and measure the same particles more than once. (wikipedia.org)
  • method
  • Our method performs dilation using Riemannian distance maps that are derived from the local structure tensor field in the image. (diva-portal.org)
  • Within the field of cognitive neuroscience, functional magnetic resonance imaging (fMRI) is a popular method of visualizing brain function. (jove.com)
  • To better understand cognitive processes, it is thus advantageous to utilize event-related potential (ERP) recording as a method of gathering information about the brain. (jove.com)
  • continuous
  • Modern microscope automation permits the collection of vast amounts of continuous anatomical imagery in both two and three dimensions. (utah.edu)
  • capture
  • However, in the quest to localize brain functions, it is relevant to note that many cognitive, sensory, and motor processes have temporal distinctions that are imperative to capture, an aspect that is left unfulfilled by fMRI's suboptimal temporal resolution. (jove.com)
  • application
  • Examples of computer-assisted developments of the technique are found in the application of "DRIMAPS" from the laboratory of Graham Dunn and other recent developments of the methodology are described by Mahlmann et al. (wikipedia.org)
  • however
  • However, with the typical procedure of AB experiments, it is not possible to examine how the processing of non-target items in RSVP may be affected by attention. (jove.com)