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In Situ HybridizationIn Situ Hybridization, FluorescenceNucleic Acid HybridizationFluorescenceSpectrometry, FluorescenceMicroscopy, FluorescenceRNA, MessengerMolecular Sequence DataFluorescence PolarizationFluorescent DyesDNA ProbesBase SequenceRNA ProbesHybridization, GeneticImmunohistochemistryFluorescence Resonance Energy TransferPolymerase Chain ReactionMicroscopyMicroscopy, ElectronAmino Acid SequenceCloning, MolecularChromosome AberrationsDigoxigeninOligonucleotide ProbesGene ExpressionChromosome MappingKaryotypingDNABlotting, NorthernGene AmplificationMicroscopy, ConfocalMicroscopy, Fluorescence, MultiphotonDNA, ComplementaryComparative Genomic HybridizationTranslocation, GeneticGene Expression Regulation, DevelopmentalChromosome BandingAneuploidySequence Analysis, DNAFluorescence Recovery After PhotobleachingPhylogenyRNA, Ribosomal, 16SReverse Transcriptase Polymerase Chain ReactionTissue DistributionTime FactorsDNA, BacterialCarcinoma in SituMicroscopy, Electron, ScanningStaining and LabelingBlotting, SouthernDNA, RibosomalGreen Fluorescent ProteinsKineticsSensitivity and SpecificityGene DosageDNA PrimersSequence Homology, Amino AcidCells, CulturedOligonucleotide Array Sequence AnalysisDNA, ViralRhodaminesAutoradiographyTranscription, GeneticLuminescent ProteinsPrimed In Situ LabelingChromosomes, Human, Pair 1Cell LineChromosomes, Human, Pair 17Nucleic Acid ProbesMolecular Probe TechniquesSpecies SpecificityParaffin EmbeddingChromosomes, Human, Pair 7RNACarbocyaninesCell NucleusCytogenetic AnalysisRats, Sprague-DawleyGene LibraryGene Expression RegulationPeptide Nucleic AcidsInterphaseLasersMicroscopy, Atomic ForceChromosomes, Human, Pair 11Chromosome DeletionGene Expression ProfilingImage Processing, Computer-AssistedEnergy TransferBrainFluorescent Antibody TechniqueHistocytochemistryFluoresceinsOrgan SpecificityChromosomes, HumanGenes, erbB-2TryptophanMutationChromosomes, Human, Pair 8Chromosomes, Human, Pair 12

AssaysMicroscopyImmunohistochemistryFluorescentFISHChromosomesChromogenicProbesDiagnosticsConfocal microscopeMRNAHistochemistryIncubatorsThermoBriteAdditionallyCellularTumorTissueZeissDetectComparativeProbeTissuesSpecimensElectronLaboratoryStructuresSlidesSignalsDetectionWhole mountImagingLymph nodeMethodsLampSampleApplicationsAnalysisFunctionSeriesImageProcess

Assays5

• An alternative technology, branched DNA assay, can be used for RNA (mRNA, lncRNA, and miRNA ) in situ hybridization assays with single molecule sensitivity without the use of radioactivity. (wikipedia.org)
• Tubule formation and three-dimensional sprouting assays and tissue fluorescence staining were conducted to investigate angiogenesis. (thno.org)
• In vitro permeability assays, trans-endothelial invasion assays, in vivo permeability assays and tissue fluorescence staining were conducted to examine vascular permeability. (thno.org)
• Mechanistically, dual luciferase reporter assays, fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP) and RNA pull-down experiments were performed to confirm the underlying mechanisms of circHERC1. (biomedcentral.com)
• The cost-effectiveness, field-portability and simplicity of this platform will create various opportunities for its use in resource limited settings and point-of-care offices, opening up a myriad of additional applications based on other fluorescence-based diagnostic assays. (chalmers.se)

Microscopy6

• Then, the probe that was labeled with either radio-, fluorescent- or antigen-labeled bases (e.g., digoxigenin) is localized and quantified in the tissue using either autoradiography, fluorescence microscopy, or immunohistochemistry, respectively. (wikipedia.org)
• Microscopy, PCR, and fluorescent in situ indicate that specific histo-blood group antigens play a hybridization provided evidence of norovirus infection. (cdc.gov)
• Each microscope is equipped with high resolution CCD cameras, dedicated workstations with access to networked drives and powerful image analysis software as well as live cell microscopy capabilities. (uvm.edu)
• carry out studies where biological structures or functions are visualised using methods based on fluorescence microscopy. (lu.se)
• understand the limitations and possibilities when using different methods based on light and fluorescence microscopy. (lu.se)
• A theoretical introduction to microscopy with an emphasis on fluorescence microscopy. (lu.se)

Immunohistochemistry3

• Additionally, advanced techniques like immunohistochemistry, fluorescence in situ hybridization (FISH), and molecular profiling can further elucidate the genetic and molecular characteristics of the tumor, guiding treatment decisions and predicting patient prognosis [ 3 ]. (alliedacademies.org)
• EGFR protein expression and EGFR gene copy number were analysed by means of immunohistochemistry and fluorescence in situ hybridisation. (biomedcentral.com)
• In addition, the in situ expression of VGLL3 in the GC tissues was determined by immunohistochemistry (IHC), and the patients were accordingly classified into the high and low expression groups. (researchgate.net)

Fluorescent3

• The key techniques currently in use include in situ hybridization to mRNA with oligonucleotide and RNA probes (both radio-labeled and hapten-labeled), analysis with light and electron microscopes, whole mount in situ hybridization, double detection of RNAs and RNA plus protein, and fluorescent in situ hybridization to detect chromosomal sequences. (wikipedia.org)
• The regulatory mechanism of lncSNHG5 was investigated by RNA sequencing, fluorescent in situ hybridization, cellular fractionation assay, mass spectrometry, RNA pull-down, RNA immunoprecipitation, gene-specific m6A assay, chromatin immunoprecipitation, dual luciferase reporter assay and actinomycin D treatment in CAFs and NFs. (thno.org)
• Fluorescence "in situ" hybridization (FISH) technique enables chromosomes to be tagged with fluorescent dyes to highlight genetic traits. (lu.se)

FISH12

• ICOE AO100F microscope is configured for FISH aka in situ hybridization. (nbicoe.com)
• It comes with DAPI, ET Gold FISH, ET Aqua FISH, and ET Green/Red FISH fluorescence filters. (nbicoe.com)
• To investigate the possibility that a novel candidate gene for XLMR was disrupted at the X chromosome translocation breakpoint, we mapped the breakpoint using fluorescence in situ hybridisation (FISH). (bmj.com)
• DSS offers a complete solution for FISH from reagents, probes, hybridization chambers to FL microscopes with accurate filters and software. (dssimage.com)
• Hybridizer is a hands-free denaturation and hybridization instrument designed for slide-based fluorescence and chromogenic in situ hybridization (FISH and CISH, respectively) on histology and cytology samples. (dssimage.com)
• HyperChrome is the perfect Instrument for denaturation & hybridization procedures during FISH experiments. (dssimage.com)
• What is Fluorescence In Situ Hybridization (FISH), and how does it work? (dssimage.com)
• Marrow samples can also be used for fluorescence in situ hybridization (FISH), flow cytometry, immunophenotyping, karyotype tests and polymerase chain reaction . (lls.org)
• By optimizing a peptide nucleic acid (PNA) based fluorescence in situ hybridization (FISH) assay, we demonstrate the use of the smartphone-based microscope for rapid identification of pathogenic bacteria. (chalmers.se)
• The PNA-based FISH assay, in combination with the smartphone-based fluorescence microscope, allowed us to qualitatively analyze pathogenic bacteria in contaminated powdered infant formula (PIF) at initial concentrations prior to cultivation as low as 10 CFU per 30 g of PIF. (chalmers.se)
• Bioinformatics analyses, the dual-luciferase reporter system, fluorescence in situ hybridization (FISH) and miRNA/mRNA high-throughput sequencing were used to identify circ-EPB41 and its downstream target. (biomedcentral.com)
• Chromogenic in situ hybridization (CISH) was developed to provide an alternative to FISH. (zytomed-systems.com)

Chromosomes2

• In situ hybridization is used to reveal the location of specific nucleic acid sequences on chromosomes or in tissues, a crucial step for understanding the organization, regulation, and function of genes. (wikipedia.org)
• It was long believed that there were 48 chromosomes in total, but no one was sure because it was difficult to obtain good microscope slides. (lu.se)

Chromogenic1

• Instead of direct fluorescence labelling of the probe, various haptens - digoxigenin, biotin, dinitrophenyl - are used which are detected in a multi-stage procedure by means of chromogenic precipitate formation. (zytomed-systems.com)

Probes2

• Subsequent signal amplification is predicated on specific hybridization of adjacent probes (individual oligonucleotides [oligos] that bind side by side on RNA targets). (wikipedia.org)
• We evaluated the use of both a general nucleic acid stain as well as species-specific PNA probes and demonstrated that the mobile platform can detect bacteria with a sensitivity comparable to that of a conventional fluorescence microscope. (chalmers.se)

Diagnostics1

• Diagnostics based on fluorescence imaging of biomolecules is typically performed in well-equipped laboratories and is in general not suitable for remote and resource limited settings. (chalmers.se)

Confocal microscope1

• After maturation, mounted retinas were stained with DAPI and imaged with a Zeiss 700 confocal microscope in order to visualize any alterations present in rod nuclei. (arvojournals.org)

MRNA2

• In situ hybridization is a powerful technique for identifying specific mRNA species within individual cells in tissue sections, providing insights into physiological processes and disease pathogenesis. (wikipedia.org)
• In response to question 2, I fixed and dehydrated the E11.5 embryos for whole mount mRNA in situ hybridization. (biologists.com)

Histochemistry1

• For hybridization histochemistry, sample cells and tissues are usually treated to fix the target transcripts in place and to increase access of the probe. (wikipedia.org)

Incubators1

• We have a Leica M 165FC stereo zoom microscope for isolating blastocysts with a Leica DFC 310 FX CCC digital image capture system, dedicated incubators, stem cell processing hoods and a colony picking hood as well as an oxygenated incubator. (uvm.edu)

ThermoBrite1

• Abbott Molecular ThermoBrite® System offers an easy, safe, system for in-situ hybridization procedures. (dssimage.com)

Additionally2

• Additionally, in an adjacent specialized BSL2 facility, viral vectors are generated, visualized and recorded using an inverted Nikon TS-100-F microscope with bright field and fluorescence capabilities with SPOT camera and SPOT Advanced software. (uvm.edu)
• Additionally, DNA fluorescence in situ hybridization against a major satellite repeat region of the genome was used to quantify the number of chromocenters within each nuclei. (arvojournals.org)

Cellular1

• Histological examination of the biopsy sample under a microscope provides valuable information about the cellular architecture, growth patterns, and differentiation status of the tumor. (alliedacademies.org)

Tumor2

• In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acids strand (i.e., probe) to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ) or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells, and in circulating tumor cells (CTCs). (wikipedia.org)
• RNA ISH (RNA in situ hybridization) is used to measure and localize RNAs (mRNAs, lncRNAs, and miRNAs) within tissue sections, cells, whole mounts, and circulating tumor cells (CTCs). (wikipedia.org)

Tissue6

• However, in situ hybridization requires that many steps be taken with precise optimization for each tissue examined and for each probe used. (wikipedia.org)
• In addition, in-situ hybridization on tissue sections require that tissue slices be very thin, usually 3 µm to 7 µm in thickness. (wikipedia.org)
• Common methods of preparing tissue sections for in-situ hybridization processing include cutting specimens with a cryostat or a Compresstome tissue slicer. (wikipedia.org)
• A pathologist (a doctor who specializes in diagnosing disease) will examine the tissue under a microscope to determine whether melanoma cells are present. (mskcc.org)
• Lymph node biopsy: A small piece of tissue is removed from a lymph node and looked at under a microscope. (mdanderson.org)
• A lab test that uses antibodies to identify and examine specific antigens in a tissue sample under a microscope, including specific cancer biomarkers. (accc-cancer.org)

Zeiss1

• DMI 6000B microscope and an upright Zeiss AxioImager Z2 microscope. (uvm.edu)

Detect1

• The samples are examined under a microscope to detect any chromosome changes (cytogenetic analysis) and other changes in the cells. (lls.org)

Comparative1

• [ 2 ] This technique has evolved throughout the years and is now largely performed by biopsy of the blastocyst trophectoderm cells with analysis using techniques such as next-generation sequencing (NGS) and comparative genomic hybridization (CGH) to test for aneuploidy. (medscape.com)

Probe2

• As a result the two subtelomeric ends became the middle of chromosome 2, which is why we get hybridization of the probe there. (washington.edu)
• and the right shows the whole mount in situ hybridization with Rapgef5 probe Structures highlighted are the heart (yellow arrows), mid brain (pink), ear (red), tip of hind limb (blue). (biologists.com)

Tissues1

• The study of the anatomy of cells and tissues using a microscope. (accc-cancer.org)

Specimens1

• These specimens can be evaluated using a normal light microscope, which is part of the equipment of any pathology department. (zytomed-systems.com)

Electron1

• The fiber surface was characterized by field emission scanning electron microscope equipped with energy dispersive X-ray analysis. (who.int)

Laboratory1

• The laboratory has upright, inverted, and dissecting microscopes with digital imaging capabilities. (uvm.edu)

Structures1

• This approach involves the intramolecular cross-linking of 5′ amine- and 3′ disulfide-modified MO oligonucleotides using appropriately functionalized tethers, generating macrocyclic structures that conformationally resist RNA hybridization. (cdc.gov)

Slides2

• Trajan's White Series 1 90° Advanced Adhesive Ground Edged Microscope Slides have a hydrophilic surface with a contact angle around 30° and strong adherence. (sciquip.co.uk)
• CultureWell glass slides and coverslips for inverted microscopes [similar to Lab Tek multi-well slides]. (ox.ac.uk)

Signals1

• Here we demonstrate the development of a compact, lightweight and cost-effective smartphone-based fluorescence microscope, capable of detecting signals from fluorescently labeled bacteria. (chalmers.se)

Detection1

• Overnight hybridization and detection on the next day. (zytomed-systems.com)

Whole mount1

• Expressions of goosecoid and no tail were detected by whole-mount in situ hybridization from 6 hpf. (bioone.org)

Imaging2

• Live Cell Imaging - The Mattek 3.5cm Petri dish & Multiwell Plates for the inverted microscope www.glassbottomdishes.com .These Petri Dishes and multiwell plates have a coverslip on the base enabling hi-power oil objectives to be used to image the specimen. (ox.ac.uk)
• 2/ 3 · use various types of advanced research microscopes, and imaging methods which are not based on optical microscopes. (lu.se)

Lymph node1

• Immunophenotyping: Cells from a lymph node, blood or bone marrow are examined with a microscope to determine what type of non-Hodgkin lymphoma cells are present. (mdanderson.org)

Methods1

• Theoretical introduction to digital visualisation with an emphasis on fluorescence-based methods and digital image processing. (lu.se)

Lamp1

• Which lamp should I use for my fluorescence microscope? (zytomed-systems.com)

Sample1

• The sample is examined under a microscope for signs of lymphoma. (healthline.com)

Applications1

• With auto fluorescence, they are also ideal for in situ hybridization (ISH) based applications. (sciquip.co.uk)

Analysis1

• Practical project: production, documentation, and analysis of a microscope preparation, with oral and written presentation. (lu.se)

Function1

• The study of the structure, function, and chemistry of cells using a microscope. (accc-cancer.org)

Series1

• Signal amplification is achieved via a series of sequential hybridization steps. (wikipedia.org)

Image1

• The image has been pseudocolored, to look like it does in the microscope. (washington.edu)

Process1

• Mercaptopropyl-functionalized stainless steel wire that was formed by co-condensation of tetramethoxysilane and 3-mercaptopropyltrimethoxysilane via a sol-gel process, which is followed by in situ surface radical chain-transfer polymerization of 1-vinyl-3-octylimidazolium hexafluorophosphate to result in polymeric ionic liquid modified stainless steel wire. (who.int)