• This newly developed approach represents a significant advance in molecular biology, opening the door to less invasive and more precise analyses of pathogenic microorganisms present in human host cells. (pasteur.fr)
  • It has always been necessary for researchers to visualise pathogenic microorganisms in their host cell's environment, in order to define the host-pathogen interactions contributing to viral infections. (pasteur.fr)
  • The high resistance of pathogenic microorganisms to different, and even the most modern antibiotics is becoming an increasingly serious problem for clinical medicine that could be decided using nanoparticles of various metals, including AuNPs. (encyclopedia.pub)
  • The occurrence of these pathogenic microorganisms was evaluated to investigate a possible health risk in the communities. (ac.ir)
  • The complexity of food matrices, the wide variety of microorganisms therein, and the varying growth and replication traits of target pathogenic microorganisms that, when present, are often in much smaller numbers than the nonpathogenic microorganisms, pose significant challenges for pathogen detection. (docksci.com)
  • A monoclonal antibody called rituximab can bind to the B lymphocyte's Clusters of Differentiation (CD) 20 antigen and eliminate B lymphocytes from the bloodstream. (ijpsonline.com)
  • A monoclonal antibody (Mab) designated as GHR106 was generated against the extracellular domain (N1-29 synthetic peptide) of human gonadotropin releasing hormone (GnRH) receptor. (annexpublishers.com)
  • Development of a monoclonal antibody immunoassay for the detection of gasoline and diesel fuel in the environment. (cdc.gov)
  • For this study, water samples were collected using a cartridge filter, eluted by washing the filter using a detergentbased medium, concentrated by centrifugation, clarified by a percoll-sucrose density gradient, stained by an indirect fluorescent antibody, and examined by epifluorescence microscopy. (ac.ir)
  • dc.title: Applications of the indirect fluorescent antibody test in malaria dc.contributor.author: SIVANESARAJAH, Y dc.description.abstract: Various methods have been employed in the epidemiological assessment of malaria. (who.int)
  • The indirect fluorescent antibody test (IFA) was the serological test employed in this laboratory. (who.int)
  • in most applications the substance in question serves as antigen, both in antibody production and in measurement of antibody by the test substance. (theodora.com)
  • Frequently used methods are based on, for example, antigen-antibody systems, nucleic acid hybridization techniques, and protein-ligand systems. (justia.com)
  • Identification of such tumor-associated cell surface antigen polypeptides, i.e. tumor associated antigens (TAA), allows specific targeting of cancer cells for destruction via antibody-based therapies. (justia.com)
  • These techniques were based on the Halogen Immunoassay (HIA), an immunostaining technique that detects antigens associated with individual airborne particles >1 microm, with human serum immunoglobulin E (IgE). (cdc.gov)
  • The Covid19 rapid test for healthcare professionals is a lateral flow chromatographic immunoassay that allows the qualitative detection of IgG and IgM antibodies against 2019-nCoV. (reallaboratory.com)
  • Immunofluorescence (IFA) is a traditional laboratory technique that utilizes fluorescent dyes to identify the presence of antibodies bound to specific antigens. (leolabs.in)
  • In various embodiments, the present invention provides fluorescent dyes that are linked to another species through an adaptor moiety. (justia.com)
  • The present invention relates generally to the synthesis of fluorescent compounds that are analogues of cyanine dyes. (justia.com)
  • Full-spectrum cytometry also allows for closely related dyes, that would not work together in traditional fluorescent cytometry, to be used in panels on these spectral cytometers. (cheekyscientist.com)
  • Thirty-five samples were assayed for the presence of enteroviruses by cell culture and 35 samples were assayed by IFA technique for the detection of cysts and oocysts. (ac.ir)
  • In-situ fluorescent immunomagnetic multiplex detection of foodborne pathogens in very low numbers. (docksci.com)
  • An exemplary method involves detection of single molecules of fluorescent labels released from a nucleic acid during synthesis of an oligonucleotide. (justia.com)
  • The collaboration has progressed to producing prototype detection elements for electrochemical and fluorescent detection. (markettactic.com)
  • The addition of new fluorescent and electrochemical detection pathways expands the scope of the AMIP technology into a larger array of device formats. (markettactic.com)
  • Specifically, the integration of the technology into a variety of sensor arrays and detection mechanisms including electrochemical and fluorescent-based sensors. (markettactic.com)
  • The cysteine engineered antibodies may be conjugated with chemotherapeutic drugs, toxins, affinity ligands such as biotin, and detection labels such as fluorophores. (justia.com)
  • Hence, this is that the detection of COVID-19 IgM antibodies tends to indicate a recent exposure to the COVID-19 virus. (reallaboratory.com)
  • Then, while the detection of COVID-19 IgG antibodies indicate a later stage of infection. (reallaboratory.com)
  • The manufacturing of monoclonal antibodies (mAbs), an important. (tudelft.nl)
  • 21:183-87) and monoclonal antibodies (mAbs) as well as drug-linking and drug-releasing properties (Lambert, J. (2005) Curr. (justia.com)
  • Of particular value are methods for measuring small quantities of nucleic acids, peptides, saccharides, pharmaceuticals, metabolites, microorganisms and other materials of diagnostic value. (justia.com)
  • When antibodies, small molecules, or nucleic acids bind to the surface they affect the current flow, which is recorded as a change in impedence in the chip that carries the sample. (genengnews.com)
  • The invention relates generally to antibodies engineered with reactive cysteine residues and more specifically to antibodies with therapeutic or diagnostic applications. (justia.com)
  • Discussing the diversity of microorganisms in an environment and microbe-microbe interactions among the populations, he pointed out the specialized functions of various microorganisms in the processes of matter circulation, and the succession of different functional groups in the community in which they work. (wikipedia.org)
  • However, the years after Mexico showed that the interest of the Committee was not restricted to the environmental problems, but extended to the central themes of microbial ecology, i.e. cycling of elements in soil and sediments, interactions between microorganisms and plants, animals, and among themselves, the effect of environmental factors on the growth and activities of microorganisms. (wikipedia.org)
  • This new imaging technique could become a key tool in the study of numerous microbial complexes and their interactions with host cells at the molecular level. (pasteur.fr)
  • In recent years, new serological techniques have suppemented the measurement of spleen rate and parasite rate. (who.int)
  • SYN: group i.. humoral i. i. associated with circulating antibodies, in contradistinction to cellular i.. infection i. the paradoxical immune status in which resistance to reinfection coincides with the persistence of the original infection. (theodora.com)
  • Whipple's disease (WD) is a rare chronic systemic infection with a wide range of clinical symptoms, routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS) diastase staining and immunohistological analysis with specific antibodies. (frontiersin.org)
  • To deal with such a complex challenge, the oral mucosal immune system must tolerate commensal microorganisms but prevent invasion of pathogens. (huji.ac.il)
  • Serology in practice typically refers to the diagnostic identification of antibodies in the serum. (eseinhealth.com)
  • Standard troponin I and hs‑TnI are determined according to the principle of immunometric technique (sandwich) on microwell technology of Vitros 5600 device with serum samples of patients received by the lab. (howest.be)
  • We have developed innovative immunodetection techniques to determine the extent to which larger fungal fragments, including hyphae and fractured conidia, function as aeroallergen sources. (cdc.gov)
  • Assays using monoclonal antibodies enable the measurement of individual antigens but interpretation can be confounded by cross-reactivity between fungal species. (cdc.gov)
  • The recent development of species-specific monoclonal antibodies, used in combination with a fluorescent-confocal HIA technique should, for the first time, enable the speciation of morphologically indiscernible fungal fragments. (cdc.gov)
  • Optical microscopy, combined with fluorescent labels (such as GFP proteins and antibodies coupled with synthetic fluorophores), allows to showcase the specific structures of cells, including proteins. (pasteur.fr)
  • This non-invasive technique allows to observe proteins without destroying or altering their biological functions. (pasteur.fr)
  • With modern genetic engineering tools, it is now possible to modify microorganisms so that their production of industrially useful proteins-such as those used in biopharmaceuticals-is enhanced. (titech.ac.jp)
  • Consequently, it is essential to resort to more precise imaging techniques, in order to better understand and define the internal structure of such viruses. (pasteur.fr)
  • Previous attempts to develop flow-through devices to continuously capture viruses in solution using conventional technology such as immobilized antibodies, have run into technical challenges that have not been solved. (markettactic.com)
  • Diagnostic and therapeutic uses for cysteine engineered antibody drug compounds and compositions are disclosed. (justia.com)
  • The invention also relates to methods of using antibodies and antibody-drug conjugate compounds for in vitro, in situ, and in vivo diagnosis or treatment of mammalian cells, or associated pathological conditions. (justia.com)
  • Introduction to Diagnostic Microbiology for the Laboratory Sciences, Second Edition provides a concise study of clinically significant microorganisms for the medical laboratory student and laboratory practitioner. (digibookee.com)
  • The new methods and techniques were proved to be successful for the analysis of microbial community in various fields, soil and water microbiology, food preservation and fermentation, and medical microbiology. (wikipedia.org)
  • Thus, there is wide interest in non-radioactive labels, particularly in labels that are observable by spectrophotometric, spin resonance, and luminescence techniques, and reactive materials, such as enzymes that produce such molecules. (justia.com)
  • Cysteine engineered anti-MUC16 antibodies are engineered by replacing one or more amino acids of a parent anti-MUC16 antibody with non cross-linked, reactive cysteine amino acids. (justia.com)
  • PALM imaging relies on the acquisition of thousands of low-resolution images, each of which showing only a few fluorescent molecules. (pasteur.fr)
  • Drug moieties used in antibody drug conjugates include bacterial protein toxins such as diphtheria toxin, plant protein toxins such as ricin, small molecules such as auristatins, geldanamycin (Mandler et al (2000) J. of the Nat. (justia.com)
  • The removal of AF helps to improve the ability to detect fluorescent signals. (cheekyscientist.com)
  • The binding of the FlAsH fluorophore to the peptide generates a fluorescent signal, thereby enabling the visualization of the protein. (pasteur.fr)
  • The Q-bodies are designed to become fluorescent upon binding to a target protein. (titech.ac.jp)
  • To see them move requires a technique like stop-motion animation: start the action, freeze the protein, capture an image, repeat. (universityofcalifornia.edu)
  • Some cytotoxic drugs tend to be inactive or less active when conjugated to large antibodies or protein receptor ligands. (justia.com)
  • Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). (cipsm.de)
  • Q-bodies are artificial antibodies which become fluorescent on binding to their target. (titech.ac.jp)
  • or it may be passive, being acquired from transfer of antibodies from another person or from an animal, either naturally, as from mother to fetus, or by intentional inoculation (artificial passive i.), and, with respect to the particular antibodies transferred, it is specific. (theodora.com)
  • It's often used in conjunction with artificial insemination (AI), a type of assisted reproductive technique. (androwash.in)
  • This test can detect the new coronavirus using the real-time RT-PCR probe method, through the reaction of the specific primer and the fluorescent probe in the sample. (reallaboratory.com)
  • Several currently available nanoprobes such as fluorescent nanoprobes, upconversion nanoparticle probes, supermagnetic iron oxide nanoprobes, and polymer- and liposome based nanoprobes are discussed. (annexpublishers.com)
  • Then, we add DNA polymerase to the soup, along with DNA building blocks, fluorescent probes and coronavirus-specific primers. (sltrib.com)
  • BrdU is a thymidine analog that gets incorporated into DNA by cells undergoing replication, enabling DNA immunocapturing using BrdU antibodies 20 . (nature.com)
  • In this article, we'll cover three main techniques: sperm washing, swim up, and density gradient centrifugation. (androwash.in)
  • To avoid this, more gentle techniques such as double density gradient centrifugation and swim-up procedures have been developed and are now widely used in clinical practice. (androwash.in)
  • B cells are therefore particularly dependent on 'quality control' mechanisms to oversee antibody production. (cipsm.de)
  • Also, differential staining methods and fluorescent antibody techniques were introduced for in-situ observation of microorganisms. (wikipedia.org)
  • Various new techniques, ranging from the determination of growth and activity of microorganisms in the environments to their isolation and characterization, were assembled in "Modern Methods in the Study of Microbial Ecology" edited by Thomas Rosswall in 1973). (wikipedia.org)
  • To facilitate probing oral mucosal immunity, we describe here methods allowing immunological analysis of murine oral tissues using flow cytometry and immunofluorescence techniques. (huji.ac.il)
  • First, we developed methods for synthesis of ultrasound-responsive, docetaxel-loaded nanodroplets (Doc-nd) by a solid dispersion technique. (annexpublishers.com)
  • Methods of design, preparation, screening, and selection of the cysteine engineered anti-MUC16 antibodies are provided. (justia.com)
  • The flexibility of the Company's AMIP technology platform has the potential to overcome these technical challenges due to the robust properties of AMIPs derived from the completely synthetic components and manufacturing techniques developed. (markettactic.com)
  • These maps, which organize cells by their genomes, will help researchers and medical professionals to better understand how and why cellular structures lead to organ functions.While researchers have long been interested in cellular maps, the ability to determine the locations of cells and their genomic blueprints has been limited by available molecular biology techniques. (weizmann-usa.org)
  • Transmembrane or otherwise tumor-associated polypeptides specifically expressed on the surface of cancer cells as compared to normal, non-cancerous cell(s) have been identified as cellular targets for cancer diagnosis and therapy with antibodies. (justia.com)
  • The IFA test was shown to be useful to study the antibody levlels of blood donors from different areas in Sri Lanka, to observe the production and persistence of malarial antibodies in man and rhesus monkeys and for epidemiological assessment of malaria in Sri Lanka. (who.int)
  • B cells use unconventional strategies for the production of a seemingly unlimited number of antibodies from a very limited amount of DNA. (cipsm.de)
  • For this reason, it is one of the techniques most used in laboratories around the world . (reallaboratory.com)
  • This study shows the challenges in making an etiologic diagnosis of CAP in pediatric population because of the poor concordance between tests and the high percentage of multiple microorganisms in healthy children. (bvsalud.org)
  • A good sperm preparation technique can help to ensure that the sample has a high percentage of viable, motile sperm, as well as considering other factors such as sperm capacitation and apoptotic state. (androwash.in)
  • When it comes to processing sperm samples, it's important to use techniques that are gentle and able to recover a high percentage of functional sperm. (androwash.in)
  • Overall, the goal of sperm processing is to obtain a sample with a high concentration of functional sperm that are capable of fertilising an egg cell, and gentle techniques are key to achieving this goal. (androwash.in)
  • It was found that when parasitaemia was high antibody titre was low and as parasitaemia declined antibody titre rose sharply. (who.int)
  • Researchers from the Institut Pasteur and CNRS have set up a new optical microscopy approach that combines two recent imaging techniques in order to visualize molecular assemblies without affecting their biological functions, at a resolution 10 times better than that of traditional microscopes. (pasteur.fr)
  • A study coordinated by Dr Christophe Zimmer(1) (Institut Pasteur/CNRS), in collaboration with Dr Nathalie Arhel(2) within the lab headed by Pr Pierre Charneau(3) (Institut Pasteur/CNRS), shows that the association of two recent imaging techniques helps obtain unique images of molecular assemblies of HIV-1 capsids, with a resolution around 10 times better than that of traditional microscopes. (pasteur.fr)
  • Pipermint, thyme, rosemary, sage and basil species which have major characteristic specialities of Lamiaceae family, have antimicrobial activities on pathogene microorganisms. (annexpublishers.com)
  • In the 20th century, particularly after the Second World War, we have seen extensive developments in the method of isolation, counting and differentiation of microorganisms in environments. (wikipedia.org)
  • Unfortunately, current screening techniques rely on multiple chemical treatments and are either too slow or too complicated. (titech.ac.jp)
  • The important role of microorganisms in natural environments was recognized by leading microbiologists in the late nineteenth century, in particular M. Beijerinck and S. Winogradsky. (wikipedia.org)
  • However, at the era of Winogradsky and Beiuerinck, the only means of discriminating and identifying microorganisms in-situ was the microscopic observation, and the quantitative analysis of the role played by each microorganism in the community was, in most cases, not attained. (wikipedia.org)
  • A similar study was conducted to find persistence of malarial antibodies in rhesus monkeys. (who.int)
  • The newly developed approach combines super-resolution PALM imaging and fluorescent FlAsH labeling. (pasteur.fr)
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
  • Thorpe, (1985) "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review," in Monoclonal Antibodies '84: Biological And Clinical Applications, A. Pinchera et al (ed.s), pp. 475-506). (justia.com)
  • The use of antibody-drug conjugates (ADC), i.e. immunoconjugates, for the local delivery of cytotoxic or cytostatic agents, i.e. drugs to kill or inhibit tumor cells in the treatment of cancer (Lambert, J. (2005) Curr. (justia.com)
  • The Company's technology allows for novel product configuration not possible with traditional testing techniques. (markettactic.com)
  • Probiotic microorganisms have valuable effects on human body and inhibition of probiotics causes many diseases. (annexpublishers.com)