• Microorganisms have been considered to be a rich source of unique bioactive compounds. (microbialtec.com)
  • Nearly three-quarters of microbial-produced bioactive compounds are from actinomycete bacteria. (microbialtec.com)
  • Health Benefits of Antioxidant Bioactive Compounds in the Fruits and Leaves of Lonicera caerulea L. and Aronia melanocarpa (Michx. (researchgate.net)
  • Elliot fruits are frequently used for their health benefits as they are rich in bioactive compounds. (researchgate.net)
  • Natural bioactive compounds derived from plant species show promising therapeutic properties to combat the emerging resistance in microbial pathogens, which can be exploited as next generation anti-infective drugs. (nature.com)
  • Cathelicidin-BF (BF-30), as a 30-mer polypeptide isolated from Bungarus fasciatus serum with a secondary structure of amphipathic α-helical conformation, exhibits outstanding anti-microbial activities [ 1 , 2 ]. (jcancer.org)
  • The search for targeted drugs for cancer treatment includes consideration of natural products, which may provide new opportunities for antitumor cytotoxicity as single agents or in combination therapy. (cancer.gov)
  • The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
  • RESULTS: The GC-MS analysis confirmed the presence of [6]-Gingerol compound, and its promising cytotoxicity IC50 was found at 81.46 ug/ml in the MTT assay. (bvsalud.org)
  • We confirm that in vitro cytotoxicity test MTT assay is able to predict in vivo toxicity, at least for aflatoxins using the prediction model. (lookformedical.com)
  • Other focuses included the determination of antioxidant activity using DPPH assay and IC 50 (Inhibitory concentration) values were also determined using broth dilution assay. (ijpsr.com)
  • The isolated compounds were then investigated for their antifungal activity using broth dilution assay. (biomedcentral.com)
  • Streptomycetes are the most widely identified group, producing a wide range of biologically active compounds. (microbialtec.com)
  • GC-MS analysis demonstrated the presence of several biologically active compounds such as Clindamycin, Phytol, Octanoic acid, and Stigmasterol which might be the reason for the therapeutic properties of the plant. (biomedcentral.com)
  • Identifying novel antimicrobial agents from plant-based compounds may result in anti-microbial drugs effective against the resistant strains. (biomedcentral.com)
  • Microbial metabolites are among the most important of the cancer chemotherapeutic agents. (microbialtec.com)
  • While all of the isolated compounds showed Cbl-b inhibitory activities, ageliferins ( 4 - 10 ) were the most potent metabolites, with IC50 values that ranged from 18 to 35 μM. (cancer.gov)
  • Ferrer Taps Hypha's Fungal Metabolites Library for Antimicrobial Compound Search. (geomar.de)
  • Hypha's MycoDiverse natural products screening library comprises a collection of low molecular weight fractions from the fermentation of higher temperate and tropical basidiomycetes and higher ascomycetes fungi, generated using a technology that stimulates the production of secondary metabolites and macromolecules. (geomar.de)
  • U.K.-based Hypha is leveraging its technologies and libraries through drug discovery collaborations and agreements, and also through services including the production of DMPK/degradation metabolites, contract fermentation, antibacterial/antifungal/antitumor bioassays, and small molecule purification and identification. (geomar.de)
  • The development of liquid chromatography-mass spectrometry (LC-MS)/mass spectrometry (MS) methods for the simultaneous detection and quantification of a broad spectrum of mycotoxins has facilitated the screening of a larger number of samples for contamination with a wide array of less well-known "emerging" mycotoxins and other metabolites. (mdpi.com)
  • In vitro drug metabolism studies, are a screening mechanism to characterize drug metabolites, elucidate their pathways, to help with further in vivo testing. (td2inc.com)
  • A few years ago, there has been a growing awareness of the medical benefits of secondary metabolites extracted from marine microorganisms by microbial fermentation [ 1 , 2 ]. (springeropen.com)
  • assists in identifying natural products and synthetic compounds that may serve as bioprobes for chemical genetics, proteomics, target validation and potential lead compounds for clinical development. (cancer.gov)
  • This review highlights the important role of Erythrina species as sources of lead compounds or new class of phytotherapeutic agents for fighting against major public health (MDR infections, cancer, diabetes, obesity) in Sub-Saharan Africa. (scialert.net)
  • The compound 5-hydroxy-7,4'-dimethoxyflavone may be partly responsible for the reported antifungal activity of C. zeyheri , and may serve as a potential source of lead compounds that can be developed as antifungal phytomedicines. (biomedcentral.com)
  • It was observed that unifloral honey samples showed "non-peroxide" anti-QS activity, which was not linearly correlated with total and individual phenolic compounds. (blogspot.com)
  • Medicinal properties can be attributed to the low-fat content, with a high proportion of unsaturated fatty acids along with high fibre content, triterpenes, phenolic compounds, sterols, eritadenine, and chitosan. (biomedcentral.com)
  • Since discovering the life-supporting drug, Penicillin, the first β -lactam antibiotic, by Sir Alexander Fleming in the 1920s, lots of progress have been developed in the antibacterial compounds production, process development and characterization of active molecules. (nature.com)
  • Antibacterial activity was evaluated by using disc and agar diffusion methods and the anti-cancer effect of the rose flower extract (RE) was examined using MTT assay in lung cancer cell line. (biomedcentral.com)
  • Previous researches revealed that BF-30 is a phenylalanine- and lysine-rich polypeptide exhibiting broad spectrum and strong antibacterial properties without drug resistance. (jcancer.org)
  • Root part of C. roseus was extracted in appropriate solvent followed by evaluation of antibacterial activity by agar well diffusion assay against a total of eight bacterial stains. (ijpsr.com)
  • The goal of this study was to isolate, identify and characterize compounds from C. zeyheri leaves which are responsible for its antifungal activity. (biomedcentral.com)
  • The combined effect of the most potent compound and an antifungal drug miconazole was investigated using the checkerboard assay. (biomedcentral.com)
  • The mechanism of action of 5-hydroxy-7,4'-dimethoxyflavone as a potent antifungal agent was investigated by determining its inhibitory activity on Candida albicans drug efflux pumps using the ciprofloxacin assay. (biomedcentral.com)
  • Since 1970s, the emergence of antifungal drug resistance has been a cause of concern in the treatment of invasive fungal infections such as candidiasis [ 1 ]. (biomedcentral.com)
  • This situation is serious because microbial infections are the most frequent opportunistic diseases occurring during HIV/aids which affected many people in Africa. (scialert.net)
  • Spanish firm Ferrer Grupo is tapping into Hypha Discovery's MycoDiverse™ library in the search for low molecular weight natural products against microbial infections. (geomar.de)
  • Over the last decade, research on antimicrobials has shifted towards an alternative approach to combat pathogens using anti-infective drugs that selectively interrupt virulence pathways to help prevent or cure bacterial infections. (nature.com)
  • Thus, there is need for better strategies to protect existing drugs and develop new classes of effective drugs to treat resistant infections. (biomedcentral.com)
  • In Africa, Combretum species are well known in traditional medicine and used for various ailments and diseases ranging from heart and worm remedies to wound dressings, treatment of the mentally ill, scorpion stings and snake bites, to fever and microbial infections [ 9 ]. (biomedcentral.com)
  • The anticancer activity of the extract was evaluated by MTT (3-[4, 5-dimethylthiazol-2-y]-2, 5-diphenyl tetrazolium bromide) assay using the A431 human skin adenocarcinoma cell line. (bvsalud.org)
  • Natural products from plants and microbial sources, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drugs discoveries due to unmatched chemical diversity 1 . (nature.com)
  • A total of four pure compounds (A-D) were isolated from C. zeyheri leaf extract. (biomedcentral.com)
  • Medicinal plants have been used extensively as crude material or as pure compounds for treating various disease conditions. (biomedcentral.com)
  • To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)
  • The phytochemical screening detected the presence of saponins, alkaloids, reducing sugars and coumarins. (researchgate.net)
  • Preliminary phytochemical screening of the crude chloroform extract revealed the presence of alkaloids, flavonoids, tannins, saponins, proteins and phenolics. (ijpsr.com)
  • The compound 5-hydroxy-7,4'-dimethoxyflavone also showed inhibition of both the drug efflux pumps (with IC 50 = 51.64 μg/ml) and the antioxidant enzymes (at 5 μM). (biomedcentral.com)
  • The present study aims at the production optimization, purification, and characterization of a potent broad-spectrum antimicrobial compound (AMC) produced by Aspergillus fumigatus nHF-01 (GenBank Ac. (nature.com)
  • Medicinal plant-based drugs have the added advantage of being simple, effective and offering a broad spectrum of activity with greater emphasis on preventive action 2 . (ijpsr.com)
  • Chemical array screening for small molecule inhibitors. (riken.jp)
  • Many microbial-originated antibiotics are available in the market, and more than 120 of the most important medicines in use are obtained from terrestrial microorganisms. (microbialtec.com)
  • There has been great interest in natural products from unexplored microbial sources, especially actinomycetes, marine ecosystems, and microorganisms. (microbialtec.com)
  • Further more, one of the most recent approaches in drug discovery from microorganisms is cocultivation. (microbialtec.com)
  • Thus, the plant may act as a potent antimicrobial drug against resistant strains. (biomedcentral.com)
  • The half-maximal inhibitory concentration (IC50) of Cd and the safe doses of SFN were determined using a methyl thiazolyl tetrazolium (MTT) assay. (lookformedical.com)
  • Screening in vivo may be feasible, if a good starting point for Medicinal Chemistry and a clear strategy for differentiation exist. (docksci.com)
  • 2012). However, many drug discovery efforts rely on screening to identify starting points for Medicinal Chemistry, and screening in vivo in most cases is not commercially or ethically viable. (docksci.com)
  • Further applications are possible in various areas of pharmacology, developments catalyzed by the use of simple enzyme assays for screening before testing in intact animals. (microbialtec.com)
  • As the years' progress, researchers are beginning to see prospects in microbial enzyme production. (frontiersin.org)
  • The testosterone secretion from TM3 cells was measured using the enzyme-linked immunosorbent assay. (lookformedical.com)
  • Description: In a biochemical assay measuring inhibition of peptide substrate phosphorylation by the test compound in the presence ot recombinant human c-FMS. (guidetomalariapharmacology.org)
  • Description: Measuring inhibition of kinase activity in a biochemical assay. (guidetomalariapharmacology.org)
  • In sub-Saharan Africa, Erythrina species are used to treat frequent parasitic and microbial diseases, inflammation, cancer, wounds. (scialert.net)
  • It is caused by numerous diseases that are accompanied by pain and inflammation, as well as by imperfection of the existing drugs aimed to provide treatment of these pathological conditions. (edu.ua)
  • Drugs of natural origin have been classified as (i) original natural products, (ii) products derived or chemically synthesized from natural products or (iii) synthetic products based on natural product structures. (microbialtec.com)
  • 1983. A radio tracer study of air-water exchange of synthetic organic compounds. (cdc.gov)
  • The developed protocol allowed for the preparation of a 12-member compound library (67 % synthetic efficiency). (edu.ua)
  • It includes also the names of 158 compounds isolated from higher plants, marine sponges and algae, fungi and snake venom. (scielo.br)
  • Derivatives of 1H-2,1-benzothiazin-4(3H)-one 2,2-dioxide are promising chemicals to search and develop drugs with the pharmacological properties required. (edu.ua)
  • Furthermore, the in silico studies used [6]-Gingerol and 21 structural analogs collected from the PubChem database to investigate the anticancer potential and drug-likeliness properties. (bvsalud.org)
  • Pingyangmycin is an anticancer drug known as bleomycin A5 (A5), discovered in the Pingyang County of Zhejiang Province of China. (lookformedical.com)
  • Also, there are increasing cases of antibiotics resistance among most causative agents of diseases in human beings, which calls for an alternative drug discovery using natural sources. (frontiersin.org)
  • It is informative in various areas, from learning about mutations to monitoring targeted drug resistance . (frontagelab.com)
  • The long-term effectiveness of most antibiotic treatments is restricted by both pathogen drug resistance and non-target effects on the host's commensal microbial community. (nature.com)
  • Anti-infective drugs that do not perturb survival or viability of bacterial pathogens should be less likely to promote resistance than conventional antibiotics 1 , 2 . (nature.com)
  • Compounds of interest include not only classical "drug-like" organic small molecules, but also peptides, proteins, and other bioactive chemical classes. (cancer.gov)
  • A large number of potential drug targets have been cloned and functionally expressed, and enormous progress has been made in the development, miniaturization and automation of cell based assays on target molecules recombinantly expressed in mammalian cell lines. (docksci.com)
  • CONCLUSION: Thus, the [6]-Gingerol and its structure analogs could be used as lead molecules against skin cancer and future drug development process. (bvsalud.org)
  • This photo-cross-linked microarray format was found to be useful not merely for ligand screening but also to study the structure-activity relationship, that is, the relationship between the structural motif (or pharmacophore) found in small molecules and its binding affinity toward a protein, by taking advantage of the nonselective nature of the photo-cross-linking process. (riken.jp)
  • Under the enhanced circumstances, it enables end-point transduction via HBV drug-resistant mutants-genomic info in order to electrochemical signals along with ultrahigh level of responsiveness, nature, along with signal-to-noise proportion, exhibiting the lowest discovery awareness right down to Two copies/μL. (g418inhibitor.com)
  • With this approach, photo-cross linked microarrays of 2000 natural products and drugs were constructed. (riken.jp)
  • 2000. Results of the L5178Y mouse lymphoma assay and the Balb/3T3 cell in vitro transformation assay for eight phthalate esters. (cdc.gov)
  • From the Georgia Cancer Registry, breast cancer survivors diagnosed during 2000-2009 at six Georgia cancer centers underwent phone interviews about their breast cancer screening behaviors and their FDRs. (cdc.gov)
  • This approach has delivered many clinical candidates but relatively few new drugs. (docksci.com)
  • To observe the effects of Asini Corii Colla, turtle carapace glue, and other drugs on the intestinal flora of nude mice with uterine fibroids model, so as to provide evidence for the clinical application of drugs. (hindawi.com)
  • Patients who are likely to respond and those who do not respond to a drug treatment during a late-stage clinical trial may be distinguished by an assay for specific biomarkers in a process termed patient stratification. (frontagelab.com)
  • The bioassay-guided separation of the extract provided ten active compounds, including three new N-methyladenine-containing diterpenoids, agelasines W-Y ( 1 - 3 ), a new bromopyrrole alkaloid, N(1)-methylisoageliferin ( 4 ), and six known ageliferin derivatives ( 5 - 10 ). (cancer.gov)
  • A cell monolayer grown on a filtered-well is used to evaluate test compound movement between the insert (apical compartment -A) and the plate well (basal compartment -B ). Passive transport through the layer is determine by A-B flux, whereas active transporter will introduce a B-A distribution of compounds between the two compartments. (td2inc.com)
  • The preliminary isolation of C. zeyheri active compounds was carried out using chromatographic techniques which include sephadex gel column chromatography, silica gel column chromatography and thin-layer chromatography (TLC). (biomedcentral.com)
  • Compound B (5-hydroxy-7,4'-dimethoxyflavone) was found to be active against Candida albicans using broth dilution method. (biomedcentral.com)
  • In last decades, biologically active natural compound (R)-goniothalamin and its derivatives received great attention from researchers. (lookformedical.com)
  • Glucans (β- d -glucans), triterpenoids, and ergosterol are key active compounds. (biomedcentral.com)
  • Albumin binding affinity test and in vitro control-released assay were performed to screen these BF-30 derivatives and explore the mechanism of anti-tumour effects. (jcancer.org)
  • It is needless to mention that though herbal plants are difficult to keep in track with the complex components and their mechanism of action, it's important to know that the complex compounds of these herbal plants work in perfect harmony almost like an orchestra. (interesjournals.org)
  • Serological tests can be one step closer to understanding the whole mechanism and its action of the compounds understanding more about therapeutic effect of these herbal plants and even continuing with advancement in modern medicine. (interesjournals.org)
  • Description: Determined in an ELISA assay detecting tyrosine phosphorylation using immobilised recombinant CSF-1R protein. (guidetomalariapharmacology.org)
  • Protein binding assays assesses the degree to which drugs attaches to proteins within the plasma/tissue. (td2inc.com)
  • Protein binding analysis by equilibrium dialysis of plasma across mutiple different speacies (mouse, rat, dog, monkely, human) following exposure to multiple compounds. (td2inc.com)
  • In 1969, Stähelin made the key decision to include a test system for immunosuppression into the general screening program at Sandoz for compounds and extracts. (wikipedia.org)
  • Although advantages and disadvantages herbal plants can be discussed according to several points, we can also mention other advantages such as its performance in the market, natural synergism, herbal shotgun approach, and isolated compounds versus herbal extracts. (interesjournals.org)
  • Viswanathan and colleagues reviewed the recent progress in the culture of hPSCs with emphasis on the importance of the environment surrounding these cells and high content analysis approaches for assay development. (docksci.com)
  • Ethnobotanical and ubiquitous plants provide a rich resource for natural drug research and development 1 . (ijpsr.com)
  • Screening of herbal plants for various therapeutic properties is the hour as it shows promising activity. (biomedcentral.com)
  • Herbal plants are a promising source of drug targets for various acute and chronic diseases. (biomedcentral.com)
  • Promising examples include anti-microbial assays, cell proliferation, platelet aggregation, and insulin release from pancreatic β-cells. (docksci.com)
  • assays indicated possible low cy to to xicity to the extract. (researchgate.net)
  • Therefore, a rationale for treating hypertension would be to administer drugs or natural compounds which selectively inhibit ACE. (scielo.br)
  • Furthermore, it is highly versatile and suitable for applications in the preclinical drug research and in vitro assays. (bvsalud.org)
  • This compound was also found to have synergistic activity on growth of C. albicans when combined with miconazole, completely inhibiting growth after only 4 hrs of incubation. (biomedcentral.com)
  • Finally, solid phase micro-extraction, coupled with gas chromatography- mass spectroscopy was utilized to identify and quantify polyphenolic compounds known to have free radical scavenging activity in the pollen samples. (blogspot.com)
  • Amongst all the marine invertebrates, sponges represent the most described group with potential application in medicinal chemistry, representing a valid starting point for new drug leads due to their chemical defense mechanisms. (microbialtec.com)
  • The search for new groups of anti-inflammatory and analgesic drugs is a topical issue of the current medicinal chemistry. (edu.ua)
  • Target based screening is likely to provide very good drug candidates for monogenic diseases, and the following collection of manuscripts is not meant to discourage the use of target based approaches. (docksci.com)
  • Here, target agnostic approaches using phenotypic assays may offer significant benefit. (docksci.com)
  • The limited throughput of phenotypic assays compared to most target-based assays necessitates smaller libraries that are optimized with regard to biological and chemical diversity. (docksci.com)
  • Wassermann and colleagues discuss strategies for building appropriate, well-annotated compound libraries. (docksci.com)
  • Microbes that synthesized polymeric compounds as polysaccharides may be found in the marine environment [ 8 , 9 ]. (springeropen.com)
  • Without a doubt, herbal plants have very high importance due to the complex compounds that function in synergy. (interesjournals.org)
  • The Molecular Targets Program (MTP) provides the focus and infrastructure that enables CCR investigators to pursue molecularly targeted drug discovery research by promoting an interdisciplinary, collaborative, team-oriented approach to identifying and validating potential cancer-pertinent targets. (cancer.gov)
  • Since the advent of molecular cloning, target based screening has become the norm in pharmaceutical drug discovery. (docksci.com)
  • Most of these drugs interact relatively weakly with a number of targets, and the complete profile of their molecular interactions is not well-known. (docksci.com)
  • The perspective by Dr. Swinney analyzes the phenotypic end points used in the discovery of new molecular entities that have resulted from phenotypic drug discovery efforts. (docksci.com)
  • The average molecular of the compounds is about 200-400 da. (geomar.de)
  • In recent years, attempts have been made to investigate a variety of indigenous drugs especially of plant origin against infectious diseases [ 3 , 4 ]. (ijpsonline.com)
  • Then again, therapy involving a high dose only reduced MMP9 level by 57%, compared to free drug suggesting that EPSS5 is a good inhibitor of the MMP9, as it brought MMP9 back to normal levels via the signaling pathway. (springeropen.com)
  • Our researchers are now extracting bacterial DNA from soil and marine habitats, cloning large fragments into, for example, bacterial artificial chromosomes, expressing in a host bacterium and screening the library for new compounds. (microbialtec.com)
  • Stähelin was also instrumental in the discovery process of several microbial products inhibiting cell proliferation, including cytochalasin B, brefeldin A, verrucarin A, anguinine and chlamydocin, which have played an important role in basic cancer research. (wikipedia.org)
  • In this case, phenotypic screening relies on an appropriate tissueor cell-based assay that can be miniaturized and used in combination with high throughput tools. (docksci.com)
  • After transfection, cell lysates are prepared for incubation with glass slides, where compound from NPDepo are immobilized in duplicate by a photo-crosslinking technique. (riken.jp)
  • A typical workflow for conventional bioprospecting and drug discovery. (microbialtec.com)
  • If you are interested in microbial drug discovery services, please contact us for more details. (microbialtec.com)
  • issues that often frustrate the present target based drug discovery programs. (docksci.com)
  • In order to limit the reliance on non-predictive animal models, screening strategies utilizing human pluripotent stem cells (hPSCs) are increasingly considered as resources for drug discovery. (docksci.com)
  • In the early phases of drug discovery, phenotypic assays can be used to further our knowledge of the disease process and to identify those end points that are most likely to translate to the clinic. (docksci.com)
  • Hypha in addition has drug discovery collaborations ongoing with companies including Domainex and Evotec, and a partnership with European ScreeningPort, which aims to evaluate use of the MycoDiverse natural products library in ESP's in-house epigenetic target assay panel. (geomar.de)
  • It was docked with 22 compounds, including [6]-Gingerol and 21 structural analogs. (bvsalud.org)
  • It has been shown that the method can be used to obtain compounds with the aforementioned structural fragments separated by one or two methylene units. (edu.ua)
  • A series of compounds containing heterocyclic cores and gem -difluorocycloalkyl substituents was obtained under conditions of the parallel synthesis (i.e., simultaneous performance of reaction procedures, treatment of the reaction mixture, and product isolation for a number of similar transformations) using the reductive amination - the "Nitrogen deletion" reaction sequence. (edu.ua)
  • Besides this, it also exhibits anti-microbial properties due to the presence of sparassol. (biomedcentral.com)
  • A number of microbial compounds capable of suppressing the immune response have been discovered. (microbialtec.com)
  • The rationale of these traditional uses in African traditional medicine was established by screening several species for biological activities. (scialert.net)
  • Some aspects of recent research with natural products directed to produce anti-hypertensive drugs are discussed. (scielo.br)